SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ac3H11_3921 FitnessBrowser__acidovorax_3H11:Ac3H11_3921 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 7 hits to proteins with known functional sites (download)

Q29551 Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial; SCOT; 3-oxoacid CoA-transferase 1; Somatic-type succinyl-CoA:3-oxoacid CoA-transferase; SCOT-s; Succinate-coenzyme A transferase; Succinyl-CoA:3-oxoacid CoA transferase; EC 2.8.3.5 from Sus scrofa (Pig) (see 3 papers)
48% identity, 96% coverage: 2:194/201 of query aligns to 310:503/520 of Q29551

query
sites
Q29551

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E344 (= E36) active site, 5-glutamyl coenzyme A thioester intermediate

Sites not aligning to the query:

1:39 modified: transit peptide, Mitochondrion
67 C→A: Decreases KM for acetoacetyl-CoA 3-fold. No effect on KM for acetoacetate, succinyl-CoA and succinate.; C→S: Decreases KM for acetoacetate and acetoacetyl-CoA 2-fold. Increases KM for succinate 2-fold. Decreases KM for succinyl-CoA nearly 4-fold. Strongly reduces catalytic activity.
235 C→S: Similar specific activity to wild-type.

3k6mC Dynamic domains of succinyl-coa:3-ketoacid-coenzyme a transferase from pig heart. (see paper)
48% identity, 96% coverage: 2:194/201 of query aligns to 257:450/466 of 3k6mC

query
sites
3k6mC

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binding glycerol: I270 (= I15), E291 (= E36), A313 (= A58), V377 (≠ A123), A380 (= A126), T382 (vs. gap), C412 (= C157), N414 (≠ K159)

Sites not aligning to the query:

binding glycerol: 25, 52, 76, 99

1o9lA Succinate:coenzyme-a transferase (pig heart)
48% identity, 96% coverage: 2:194/201 of query aligns to 258:451/468 of 1o9lA

query
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1o9lA

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Sites not aligning to the query:

binding 2-(ethylmercuri-thio)-benzoic acid: 87, 94, 95, 96, 131, 132, 145

3oxoE Succinyl-coa:3-ketoacid coa transferase from pig heart covalently bound to coa (see paper)
48% identity, 96% coverage: 2:194/201 of query aligns to 253:446/462 of 3oxoE

query
sites
3oxoE

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binding coenzyme a: I266 (= I15), G267 (= G16), I268 (≠ Q17), E287 (= E36), G343 (= G92), M345 (≠ Y94), N355 (= N104), I358 (≠ T107), M366 (≠ V116), A369 (= A119), K389 (= K138)

8i40A Crystal structure of asct from trypanosoma brucei in complex with coa.
44% identity, 97% coverage: 2:195/201 of query aligns to 261:453/467 of 8i40A

query
sites
8i40A

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binding coenzyme a: I274 (= I15), I276 (≠ Q17), E295 (= E36), G351 (= G92), L353 (≠ Y94), N363 (= N104), G376 (= G118), K401 (= K143)

8i3yD Crystal structure of asct from trypanosoma brucei in complex with succinyl-coa.
44% identity, 97% coverage: 2:195/201 of query aligns to 265:457/471 of 8i3yD

query
sites
8i3yD

A

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Sites not aligning to the query:

binding calcium ion: 201, 229

8i3yA Crystal structure of asct from trypanosoma brucei in complex with succinyl-coa.
44% identity, 97% coverage: 2:195/201 of query aligns to 255:447/459 of 8i3yA

query
sites
8i3yA

A

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N

G

G

A

M

Y

Y

V

V

N

N

L

L

G
|
G

I
|
I

G
|
G

Q

I

P

P

T

T

L

E

V

S

A

S

N

N

H

Y

I

I

P

P

E

A

G

G

R

V

E

N

V

V

I

V

L

L

Q

Q

S

S

E
|
E

N

N

G

G

I

L

L

I

G

G

M

M

G

G

P

P

A

F

P

P

A

T

A

E

G

D

L

K

E

V

D

D

Y

A

D

D

L

W

I

I

N

N

A
|
A

G

G

K
|
K

Q

Q

P

T

V

I

T

S

L

H

L

L

P

A

G

G

G

S

A

A

Y

L

F

F

H

D

H

S

A

A

D

T

S

S

F

F

A

A

M

M

M

I

R

R

G

G

H

H

L

M

D

D

I

L

C

T

V

M

L

L

G

G

A

A

Y

L

Q

E

V

V

S

A

A

A

T

N

G

G

D

D

L

L

A

A

N
|
N

W

F

S

M

T

I

G

-

E

-

P

P

G

G

A

K

-

L

I

V

P

K

A

G

V

P

G
|
G

A
|
A

M

M

D

D

L

L

A

V

I

S

G

C

A

G

K

T

Q

R

T

V

W

V

V

V

M

T

D

T

L

H

L

C

T
x
N

K
|
K

Q

N

G

G

A

D

S

P

K
|
K

I

I

V

V

T

E

Q

R

C

C

T

R

Y

L

P

P

L

V

T

T

G

G

V

K

A

H

C

C

V

V

K

C

R

R

I

I

Y

I

T

T

D

E

L

Y

C

A

T

V

L

F

A

D

C

V

T

V

P

D

T

G

G

R

L

L

Q

V

L

L

I

K

D

E

T

I

V

A

P

E

G

D

L

T

S

T

Q

V

T

D

E

Q

L

V

E

K

Q

K

L

L

V

T

G

G

L

V

binding succinyl-coenzyme a: G267 (= G14), I268 (= I15), G269 (= G16), E289 (= E36), A311 (= A58), K313 (= K60), N357 (= N104), G369 (= G117), G370 (= G118), A371 (= A119), N389 (≠ T137), K390 (= K138), K395 (= K143)

Sites not aligning to the query:

Query Sequence

>Ac3H11_3921 FitnessBrowser__acidovorax_3H11:Ac3H11_3921
VAQDIHDGAYVNLGIGQPTLVANHIPEGREVILQSENGILGMGPAPAAGLEDYDLINAGK
QPVTLLPGGAYFHHADSFAMMRGGHLDICVLGAYQVSATGDLANWSTGEPGAIPAVGGAM
DLAIGAKQTWVMMDLLTKQGASKIVTQCTYPLTGVACVKRIYTDLCTLACTPTGLQLIDT
VPGLSQTELEQLVGLPIRPAA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory