SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ac3H11_4184 Aldehyde dehydrogenase B (EC 1.2.1.22) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P25526 Succinate-semialdehyde dehydrogenase [NADP(+)] GabD; SSDH; Glutarate-semialdehyde dehydrogenase; EC 1.2.1.79; EC 1.2.1.- from Escherichia coli (strain K12) (see paper)
59% identity, 97% coverage: 11:492/498 of query aligns to 3:481/482 of P25526

query
sites
P25526

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WN 156:157 (= WN 162:163) binding
KPAS 180:183 (≠ KPAE 186:189) binding
GS 233:234 (= GS 242:243) binding
L256 (= L265) binding
E386 (= E397) binding

3jz4A Crystal structure of e. Coli NADP dependent enzyme (see paper)
59% identity, 97% coverage: 11:492/498 of query aligns to 2:480/481 of 3jz4A

query
sites
3jz4A

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active site: N156 (= N163), K179 (= K186), E254 (= E264), C288 (= C298), E385 (= E397), E462 (= E474)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I152 (= I159), P154 (= P161), W155 (= W162), N156 (= N163), K179 (= K186), A181 (= A188), S182 (≠ E189), S211 (≠ D218), A212 (≠ S219), G213 (≠ D220), G216 (= G226), F230 (= F240), T231 (= T241), G232 (= G242), S233 (= S243), I236 (≠ V246), E254 (= E264), L255 (= L265), C288 (= C298), K338 (= K348), E385 (= E397), F387 (= F399)

P51649 Succinate-semialdehyde dehydrogenase, mitochondrial; Aldehyde dehydrogenase family 5 member A1; NAD(+)-dependent succinic semialdehyde dehydrogenase; EC 1.2.1.24 from Homo sapiens (Human) (see 5 papers)
57% identity, 96% coverage: 16:494/498 of query aligns to 58:535/535 of P51649

query
sites
P51649

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A

F

F

A

A

-

E

A

A

K

M

V

K

K

K

T

N

A

L

K

R

V

V

G

G

N
|
N

G

G

F

F

E

E

A

E

G

G

V

T

N

T

Q

Q

G

G

P
|
P

L

L

I

I

E

N

E

E

A

K

A

A

L

V

T

E

K

K

V

V

Q

E

R

K

H

Q

V

V

D

N

D

D

A

A

L

V

A

S

K

K

G

G

G

A

Q

T

V
|
V

V

V

A

T

G
|
G

G

G

Q

K

R

R

L

-

T

H

A

Q

L

L

G

G

S

K

G

-

Q

N

F

F

E

E

P

P

T

T

V

L

A

C

N

N

A

V

T

T

A

Q

D

D

M

M

L

L

C

C

A

T

R

H

E

E

T

T

F

F

G

G

P

P

F

L

A

A

P

P

V

V

F

I

K

K

F

F

K

D

T

T

E

E

Q

E

E

E

A

A

I

I

D

A

A

I

A

A

N

N

N

A

T

A

E

D

F

V

G

G

L

L

A

A

S

G

Y

Y

F

F

Y

Y

S

S

R

Q

D

D

V

P

G

A

R

Q

I

I

F

W

R

R

V

V

T

A

E

E

A

Q

L

L

E

E

Y

V

G

G

M

M

V

V

G

G

A

V

N

N

V

E

G

G

I

L

L

I

A
x
S

T

S

E

V

H

E

V

C

P

P

F

F

G

G

V

V

K

K

Q

Q

S

S

G

G

L

L

G

G

R

R

E

E

G

G

S

S

H

K

H

Y

G

G

M

I

D

D

D

E

Y

Y

V

L

E

E

I

L

K

K

Y

Y

L

V

C

C

L

Y

G
|
G

D

G

I

L

C93 (≠ L51) to F: in SSADHD; 3% of activity; dbSNP:rs765561257
G176 (= G134) to R: in SSADHD; <1% of activity; dbSNP:rs72552281
H180 (≠ P138) to Y: 83% of activity; dbSNP:rs2760118
P182 (≠ F140) to L: 48% of activity; dbSNP:rs3765310
R213 (= R171) binding ; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
C223 (= C181) to Y: in SSADHD; 5% of activity; dbSNP:rs72552282
KPAE 228:231 (= KPAE 186:189) binding
T233 (= T191) to M: in SSADHD; 4% of activity; dbSNP:rs1326526453
A237 (= A195) to S: 65% of activity; dbSNP:rs62621664
N255 (= N213) to S: in SSADHD; 17% of activity; dbSNP:rs145087265
G268 (= G226) to E: in SSADHD; <1% of activity; dbSNP:rs375628463
GSTTTG 284:289 (≠ GSTEVG 242:247) binding
R334 (= R292) binding ; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
N335 (= N293) to K: in SSADHD; 1% of activity; dbSNP:rs72552283
C340 (= C298) modified: Disulfide link with 342, In inhibited form
C342 (= C300) modified: Disulfide link with 340, In inhibited form; mutation to A: Loss of regulation by redox state.
N372 (= N329) natural variant: N -> S
P382 (= P339) to L: in SSADHD; 2% of activity
V406 (= V363) to I: in dbSNP:rs143741652
G409 (= G366) to D: in SSADHD; <1% of activity; dbSNP:rs118203984
S498 (≠ A457) binding ; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
G533 (= G492) to R: in SSADHD; <1% of activity; dbSNP:rs72552284

Sites not aligning to the query:

36 G → R: no effect on succinate-semialdehyde dehydrogenase activity; dbSNP:rs4646832

2w8rA The crystal structure of human ssadh in complex with NAD+ (see paper)
57% identity, 96% coverage: 16:494/498 of query aligns to 8:485/485 of 2w8rA

query
sites
2w8rA

L

L

K

R

T

T

D

D

G

S

L

F

I

V

N

G

G

G

Q

R

W

W

V

L

V

P

G

A

S

A

R

T

F

F

D

P

V

V

N

Q

D

D

P

P

A

A

T

S

G

G

L

A

K

A

L

L

A

G

D

M

V

V

A

A

N

D

L

C

G

G

P

V

A

R

D

E

A

A

E

R

A

A

I

V

A

R

A

A

N

Y

A

E

A

A

W

F

G

C

P

R

W

W

K

R

T

E

K

V

T

S

A

A

K

K

E

E

R

R

S

S

I

S

I

L

L

L

R

R

K

K

W

W

F

Y

D

N

L

L

L

M

M

I

A

Q

N

N

Q

K

D

D

L

L

G

A

R

R

I

I

M

I

T

T

A

A

E

E

Q

S

G

G

K

K

P

P

L

L

A

K

E

E

A

A

K

H

G

G

E

E

V

I

A

L

Y

Y

G

S

A

A

S

F

F

F

V

L

E

E

W

W

F

F

A

S

E

E

A

A

K

R

R

R

I

V

N

Y

G

G

E

D

T

I

L

I

P

H

Q

T

F

P

D

A

N

K

N

D

R

R

L

A

M

L

V

V

L

L

K

K

Q

Q

P

P

I

I

G

G

V

V

C

A

A

A

A

V

I
|
I

T
|
T

P
|
P

W
|
W

N
|
N

F

F

P

P

L

S

A

A

M

M

I

I

T

T

R

R

K

K

V

V

A

G

P

A

A

A

L

L

A

A

G

G

C

C

P

T

V

V

I

V

K
|
K

P
|
P

A
|
A

E
|
E

L

D

T

T

P

P

L

F

T

S

A

A

L

L

A

A

A

L

A

A

E

E

L

L

A

A

I

S

R

Q

A

A

G

G

I

I

P

P

A

S

G

G

V

V

F

Y

N

N

I

V

L

I

P

P

A

C

D

S

S

R

D

K

N

N

S
x
A

I
x
K

A

E

I

V

G

G

K

E

V

A

L

I

C

C

A

T

S

D

D

P

V

L

V

V

R

S

H

K

I

I

S

S

F
|
F

T

T

G

G

S
|
S

T

T

E

T

V
x
T

G

G

R

K

I

I

L

L

M

L

A

H

Q

H

S

A

A

A

P

N

T

S

V

V

K

K

K

R

M

V

S

S

L

M

E
|
E

L

L

G

G

N

L

A

A

P

P

F

F

I

I

V

V

F

F

D

D

D

S

A

A

D

N

I

V

D

D

S

Q

A

A

V

V

E

A

G

G

A

A

F

M

A

A

S

S

K

K

Y

F

R

R

N

N

A

T

G

G

Q

Q

T

T

C
x
A

V

V

C

C

T

S

N

N

R

Q

F

F

Y

L

V

V

Q

Q

E

R

G

G

V

I

Y

H

D

D

E

A

F

F

V

V

A

K

K

A

F

F

A

A

-

E

A

A

K

M

V

K

K

K

T

N

A

L

K

R

V

V

G

G

N

N

G

G

F

F

E

E

A

E

G

G

V

T

N

T

Q

Q

G

G

P

P

L

L

I

I

E

N

E

E

A

K

A

A

L

V

T

E

K

K

V

V

Q

E

R

K

H

Q

V

V

D

N

D

D

A

A

L

V

A

S

K

K

G

G

G

A

Q

T

V

V

A

T

G

G

Q

K

R

R

L

-

T

H

A

Q

L

L

G

G

S

K

G

-

Q

N

F

F

E

E

P

P

T

T

V

L

A

C

N

N

A

V

T

T

A

Q

D

D

M

M

L

L

C

C

A

T

R

H

E

E

E
|
E

T

T

F

F

G

G

P

P

F

L

A

A

P

P

V

V

F

I

K

K

F

F

K

D

T

T

E

E

Q

E

E

E

A

A

I

I

D

A

A

I

A

A

N

N

N

A

T

A

E

D

F

V

G

G

L

L

A

A

S

G

Y

Y

F

F

Y

Y

S

S

R

Q

D

D

V

P

G

A

R

Q

I

I

F

W

R

R

V

V

T

A

E

E

A

Q

L

L

E

E

Y

V

G

G

M

M

V

V

G

G

A

V

N

N

V

E

G

G

I

L

L

I

A

S

T

S

E

V

H

E

V

C

P

P

F

F

G

G

V

V

K

K

Q

Q

S

S

G

G

L

L

G

G

R

R

E
|
E

G

G

S

S

H

K

H

Y

G

G

M

I

D

D

D

E

Y

Y

V

L

E

E

I

L

K

K

Y

Y

L

V

C

C

L

Y

G

G

D

G

I

L

active site: N155 (= N163), K178 (= K186), E256 (= E264), A290 (≠ C298), E388 (= E397), E465 (= E474)
binding adenosine-5'-diphosphate: I151 (= I159), T152 (= T160), P153 (= P161), W154 (= W162), K178 (= K186), P179 (= P187), A180 (= A188), E181 (= E189), A214 (≠ S222), K215 (≠ I223), F232 (= F240), S235 (= S243), T238 (≠ V246)

2w8qA The crystal structure of human ssadh in complex with ssa. (see paper)
57% identity, 96% coverage: 16:494/498 of query aligns to 8:485/485 of 2w8qA

query
sites
2w8qA

L

L

K

R

T

T

D

D

G

S

L

F

I

V

N

G

G

G

Q

R

W

W

V

L

V

P

G

A

S

A

R

T

F

F

D

P

V

V

N

Q

D

D

P

P

A

A

T

S

G

G

L

A

K

A

L

L

A

G

D

M

V

V

A

A

N

D

L

C

G

G

P

V

A

R

D

E

A

A

E

R

A

A

I

V

A

R

A

A

N

Y

A

E

A

A

W

F

G

C

P

R

W

W

K

R

T

E

K

V

T

S

A

A

K

K

E

E

R

R

S

S

I

S

I

L

L

L

R

R

K

K

W

W

F

Y

D

N

L

L

L

M

M

I

A

Q

N

N

Q

K

D

D

L

L

G

A

R

R

I

I

M

I

T

T

A

A

E

E

Q

S

G

G

K

K

P

P

L

L

A

K

E

E

A

A

K

H

G

G

E

E

V

I

A

L

Y
|
Y

G

S

A

A

S

F

F

F

V

L

E

E

W

W

F

F

A

S

E

E

A

A

K

R

R

R

I

V

N

Y

G

G

E

D

T

I

L

I

P

H

Q

T

F

P

D

A

N

K

N

D

R

R

L

A

M

L

V

V

L

L

K

K

Q

Q

P

P

I

I

G

G

V

V

C

A

A

A

A

V

I

I

T

T

P

P

W

W

N
|
N

F
|
F

P

P

L

S

A

A

M

M

I

I

T

T

R
|
R

K

K

V

V

A

G

P

A

A

A

L

L

A

A

G

G

C

C

P

T

V

V

I

V

K
|
K

P

P

A

A

E

E

L

D

T

T

P

P

L

F

T

S

A

A

L

L

A

A

A

L

A

A

E

E

L

L

A

A

I

S

R

Q

A

A

G

G

I

I

P

P

A

S

G

G

V

V

F

Y

N

N

I

V

L

I

P

P

A

C

D

S

S

R

D

K

N

N

S

A

I

K

A

E

I

V

G

G

K

E

V

A

L

I

C

C

A

T

S

D

D

P

V

L

V

V

R

S

H

K

I

I

S

S

F

F

T

T

G

G

S

S

T

T

E

T

V

T

G

G

R

K

I

I

L

L

M

L

A

H

Q

H

S

A

A

A

P

N

T

S

V

V

K

K

K

R

M

V

S

S

L

M

E
|
E

L

L

G

G

N

L

A

A

P

P

F

F

I

I

V

V

F

F

D

D

D

S

A

A

D

N

I

V

D

D

S

Q

A

A

V

V

E

A

G

G

A

A

F

M

A

A

S

S

K

K

Y

F

R
|
R

N

N

A

T

G

G

Q

Q

T

T

C
x
A

V
|
V

C

C

T

S

N

N

R

Q

F

F

Y

L

V

V

Q

Q

E

R

G

G

V

I

Y

H

D

D

E

A

F

F

V

V

A

K

K

A

F

F

A

A

-

E

A

A

K

M

V

K

K

K

T

N

A

L

K

R

V

V

G

G

N

N

G

G

F

F

E

E

A

E

G

G

V

T

N

T

Q

Q

G

G

P

P

L

L

I

I

E

N

E

E

A

K

A

A

L

V

T

E

K

K

V

V

Q

E

R

K

H

Q

V

V

D

N

D

D

A

A

L

V

A

S

K

K

G

G

G

A

Q

T

V

V

A

T

G

G

Q

K

R

R

L

-

T

H

A

Q

L

L

G

G

S

K

G

-

Q

N

F

F

E

E

P

P

T

T

V

L

A

C

N

N

A

V

T

T

A

Q

D

D

M

M

L

L

C

C

A

T

R

H

E

E

E
|
E

T

T

F

F

G

G

P

P

F

L

A

A

P

P

V

V

F

I

K

K

F

F

K

D

T

T

E

E

Q

E

E

E

A

A

I

I

D

A

A

I

A

A

N

N

N

A

T

A

E

D

F

V

G

G

L

L

A

A

S

G

Y

Y

F

F

Y

Y

S

S

R

Q

D

D

V

P

G

A

R

Q

I

I

F

W

R

R

V

V

T

A

E

E

A

Q

L

L

E

E

Y

V

G

G

M

M

V

V

G

G

A

V

N

N

V

E

G

G

I

L

L

I

A
x
S

T

S

E

V

H

E

V

C

P

P

F
|
F

G

G

V

V

K

K

Q

Q

S

S

G

G

L

L

G

G

R

R

E
|
E

G

G

S

S

H

K

H

Y

G

G

M

I

D

D

D

E

Y

Y

V

L

E

E

I

L

K

K

Y

Y

L

V

C

C

L

Y

G

G

D

G

I

L

active site: N155 (= N163), K178 (= K186), E256 (= E264), A290 (≠ C298), E388 (= E397), E465 (= E474)
binding succinic acid: Y109 (= Y117), F156 (= F164), R163 (= R171), E256 (= E264), R284 (= R292), A290 (≠ C298), V291 (= V299), S448 (≠ A457), F454 (= F463)

4pz2B Structure of zm aldh2-6 (rf2f) in complex with NAD (see paper)
40% identity, 96% coverage: 10:487/498 of query aligns to 2:482/494 of 4pz2B

query
sites
4pz2B

L

L

L

F

N

D

D

V

P

P

T

E

L

I

L

R

K

F

T

T

D

K

G

L

L

F

I

I

N

N

G

G

Q

S

W

F

V

V

-

D

-

A

V

V

G

S

S

G

S

R

R

T

F

F

D

D

V

T

N

R

D

D

P

P

A

R

T

T

G

G

L

G

K

V

L

I

A

A

D

S

V

V

A

A

N

E

L

A

G

D

P

K

A

E

D

D

A

V

E

D

A

L

A

A

I

V

A

R

A

A

N

R

A

A

W

F

-

D

-

H

G

G

P

E

W

W

K

P

T

R

K

M

T

S

A

G

K

S

E

E

R

R

S

G

I

R

I

I

L

M

R

A

K

R

W

L

F

A

D

D

L

L

L

V

M

E

A

E

N

H

Q

A

D

G

D

E

L

L

G

A

R

A

I

L

M

E

T

S

A

L

E

D

Q

A

G

G

K

K

-

H

P

P

L

A

A

V

E

T

A

R

K

A

G

V

E

D

V

V

A

G

Y

N

G

A

A

A

S

G

F

S

V

L

E

R

W

Y

F

F

A

A

E

G

E

A

A

A

K

D

R

K

I

I

N

H

G

G

E

E

T

T

L

L

P

K

-

M

-

P

-

G

Q

Q

F

F

D

Q

N

G

N

H

R

-

L

-

M

-

V

T

L

L

K

R

Q

E

P

P

I

L

G

G

V

V

C

A

A

G

A

V

I
|
I

T
x
I

P
|
P

W
|
W

N
|
N

F

F

P

P

L

S

A

T

M
|
M

I

F

T

A

R

V

K

K

V

V

A

A

P

P

A

A

L

L

A

A

G

G

C

C

P

A

V

L

V

V

I

V

K
|
K

P
|
P

A
|
A

E
|
E

L

Q

T

T

P

P

L

L

T

S

A

A

L

L

A

Y

A

L

A

A

E

Q

L

L

A

A

I

K

R

Q

A

A

G

G

I

V

P

P

A

D

G

G

V

V

F

I

N

N

I

V

L

V

P

P

A

G

D

F

S
x
G

D
x
P

N

T

S

A

I

-

A

-

I

-

G
|
G

K
x
A

V

A

L

L

C

A

A

S

S

H

D

M

V

D

V

V

R

D

H

M

I

V

S

S

F
|
F

T
|
T

G
|
G

S
|
S

T

T

E

E

V
|
V

G

G

R

R

I
x
L

L
x
I

M

M

A

K

Q

A

S

S

A

A

P

E

T

S

-

N

V

L

K

K

K

P

M

V

S

Y

L

L

E
|
E

L
|
L

G
|
G

G

G

N

K

A

S

P

P

F

L

I

I

V

V

F

F

D

D

A

A

D

D

I

L

D

D

S

M

A

A

V

V

E

E

G

L

A

A

F

V

A

G

S

A

K

S

Y

F

R

F

N

N

A

K

G

G

Q

E

T

A

C
|
C

V

V

C

A

T

A

N

S

R

R

F

V

Y

Y

V

V

Q

Q

E

E

G

R

V

V

Y

Y

D

D

E

R

F

F

V

E

A

E

K

R

F

L

A

A

A

E

K

R

V

M

K

R

T

S

A

W

K

V

V

V

G

G

N

D

G

P

F

F

-

S

E

D

A

P

G

R

V

A

N

D

Q

Q

G

G

P

P

L

Q

I

V

E

D

E

K

A

A

A

Q

L

Y

T

E

K

R

V

V

Q

L

R

S

H

Y

V

I

D

D

D

H

A

G

L

K

A

R

K

E

G

G

G

A

Q

T

V

L

A

T

G

G

Q

R

R

P

L

C

T

A

A

P

L

E

G

G

S

K

G

G

Q

Y

F

Y

F

I

E

E

P

P

T

T

V

V

V

F

A

T

N

N

A

V

T

K

A

E

D

D

M

M

L

I

C

I

A

A

R

K

E

E

E
|
E

T

I

F
|
F

G

G

P

P

F

V

A

M

P

C

V

L

F

M

K

K

F

F

K

K

T

T

E

V

Q

E

E

E

A

A

I

I

D

A

A

R

A

A

N

N

N

D

T

T

E

R

F

Y

G

G

L
|
L

A

G

S

A

Y

G

F

V

Y

V

S

T

R

R

D

D

V

L

G

D

R

V

I

A

F

N

R

R

V

V

T

V

E

R

A

S

L

V

E

R

Y

A

G

G

M

V

V

V

G

W

A

V

N

N

V

C

G

Y

I

F

L

A

A

M

T

G

E

S

H

D

V

C

P

P

F
|
F

G

G

V

R

K

K

Q

M

S

S

G

G

L

F

G

G

R

K

E
x
D

G

E

S

G

H

M

H

H

G

A

M

L

D

D

D

K

Y

Y

V

L

E

A

I

V

K

K

active site: N159 (= N163), K182 (= K186), E258 (= E264), C292 (= C298), E392 (= E397), D469 (≠ E474)
binding nicotinamide-adenine-dinucleotide: I155 (= I159), I156 (≠ T160), P157 (= P161), W158 (= W162), N159 (= N163), M164 (= M168), K182 (= K186), P183 (= P187), A184 (= A188), E185 (= E189), G215 (≠ S219), P216 (≠ D220), G219 (= G226), A220 (≠ K227), F233 (= F240), T234 (= T241), G235 (= G242), S236 (= S243), V239 (= V246), L242 (≠ I249), I243 (≠ L250), E258 (= E264), L259 (= L265), G260 (= G266), C292 (= C298), E392 (= E397), F394 (= F399), L420 (= L425), F458 (= F463)

5x5uA Crystal structure of alpha-ketoglutarate-semialdehyde dehydrogenase (kgsadh) complexed with NAD (see paper)
39% identity, 96% coverage: 15:491/498 of query aligns to 1:474/476 of 5x5uA

query
sites
5x5uA

T

T

L

Y

L

T

K

D

T

T

D

Q

G

L

L

L

I

I

N

D

G

G

Q

E

W

W

V

V

V
x
D

G
x
A

S
x
A

S

S

-
x
G

-

K

R

T

F

I

D

D

V

V

N

V

D

N

P

P

A

A

T

T

G

G

L

K

K

P

L

I

A

G

D

R

V

V

A

A

N

H

L

A

G

G

P

I

A

A

D

D

A

L

E

D

A

R

A

A

I

L

A

A

N

Q

A

S

A

G

W

F

G

E

P

A

W

W

K

R

T

K

K

V

T

P

A

A

K

H

E

E

R

R

S

A

I

A

I

T

L

M

R

R

K

K

W

A

F

A

D

A

L

L

L

V

M

R

A

E

N

R

Q

A

D

D

D

A

L

I

G

A

R

Q

I

L

M

M

T

T

A

Q

E

E

Q

Q

G

G

K

K

P

P

L

L

A

T

E

E

A

A

K

R

G

V

E

E

V

V

A

L

Y

S

G

A

A

A

S

D

F

I

V

I

E

E

W

W

F

F

A

A

E

D

E

E

A

G

K

R

R

R

I

V

N

Y

G

G

E

R

T

I

L

V

P

P

Q

P

F

R

D

N

N

L

N

G

R

A

R

Q

L

Q

M

T

V

V

L

V

K

K

Q

E

P

P

I

V

G

G

V

P

C

V

A

A

I

F

T

T

P
|
P

W
|
W

N
|
N

F

F

P

P

L

V

A

N

M

Q

I

V

T

V

R

R

K

K

V

L

A

S

P

A

A

A

L

L

A

A

A

T

G

G

C

C

P

S

V

F

V

L

I

V

K
|
K

P

A

A

P

E

E

L

E

T

T

P

P

L

A

T

S

A

P

L

A

A

A

A

L

E

R

L

A

A

F

I

V

R

D

A

A

G

G

I

V

P

P

A

A

G

G

V

V

F

I

N

G

I

L

L

V

P

Y

A

G

D

D

S
x
P

D
x
A

N

E

S

-

I

-

A

-

I

I

G
x
S

K

S

V

Y

L

L

C

I

A

P

S

H

D

P

V

V

V

I

R

R

H

K

I

V

S

T

F
|
F

T
|
T

G
|
G

S
|
S

T

T

E

P

V
|
V

G

G

R

K

I

Q

L
|
L

M

A

A

S

Q

L

S

A

A

G

P

L

T

H

V

M

K

K

K

R

M

A

S

T

L

M

E
|
E

L
|
L

G

G

N

H

A

A

P

P

F

V

I

I

V

V

F

A

D

E

D

D

A

A

D

D

I

V

D

A

S

L

A

A

V

V

E

K

G

A

A

A

F

G

A

G

S

A

K

K

Y

F

R

R

N

N

A

A

G

G

Q

Q

T

V

C
|
C

V

I

C

S

T

P

N

T

R

R

F

F

Y

L

V

V

Q

H

E

N

G

S

V

I

Y

R

D

D

E

E

F

F

V

T

A

R

K

A

F

L

A

V

A

K

K

H

V

A

K

E

T

G

A

L

K

K

V

V

G

G

N

N

G

G

F

L

E

E

A

E

G

G

V

T

N

T

Q

L

G

G

P

A

L

L

I

A

E

N

E

P

A
x
R

L

L

T

T

K

A

V

M

Q

A

R

S

H

V

V

I

D

D

D

N

A

A

L

R

A

K

K

V

G

G

G

A

Q

S

V

I

V

E

A

T

G

G

Q

E

R

R

L

I

T

G

A

S

L

-

G

-

S

E

G

G

Q

N

F

F

E

A

P

P

T

T

V

V

V

I

A

A

N

N

A

V

T

P

A

L

D

D

M

A

L

D

C

V

A

F

R

N

E

N

E
|
E

T

P

F
|
F

G

G

P

P

F

V

A

A

P

A

V

I

F

R

K

G

F

F

K

D

T

K

E

L

Q

E

E

E

A

A

I

I

D

A

A

E

A

A

N

N

N

R

T

L

E

P

F

F

G

G

L

L

A

A

S

G

Y

Y

F

A

Y

F

S

T

R

R

D

S

V

F

G

A

R

N

I

V

F

H

R

L

V

L

T

T

E

Q

A

R

L

L

E

E

Y

V

G

G

M

M

V

L

G

W

A

I

N

N

V

Q

G

P

I

A

L

T

A

P

T

W

E

P

H

E

V

M

P

P

F

F

G

G

V

V

K

K

Q

D

S

S

G

G

L

Y

G

G

R

S

E
|
E

G

G

S

G

H

P

H

E

G

A

M

L

D

E

D

P

Y

Y

V

L

E

V

I

T

K

K

Y

S

L

V

C

T

L

V

active site: N151 (= N163), K174 (= K186), E249 (= E264), C283 (= C298), E380 (= E397), E457 (= E474)
binding glycerol: D15 (≠ V29), A16 (≠ G30), A17 (≠ S31), G19 (vs. gap)
binding nicotinamide-adenine-dinucleotide: P149 (= P161), W150 (= W162), N151 (= N163), P207 (≠ S219), A208 (≠ D220), S211 (≠ G226), F225 (= F240), T226 (= T241), G227 (= G242), S228 (= S243), V231 (= V246), L235 (= L250), E249 (= E264), L250 (= L265), C283 (= C298), R329 (≠ A344), R330 (≠ A345), E380 (= E397), F382 (= F399)

5x5tA Crystal structure of alpha-ketoglutarate semialdehyde dehydrogenase (kgsadh) from azospirillum brasilense (see paper)
39% identity, 96% coverage: 15:491/498 of query aligns to 1:474/476 of 5x5tA

query
sites
5x5tA

T

T

L

Y

L

T

K

D

T

T

D

Q

G

L

L

L

I

I

N

D

G

G

Q

E

W

W

V

V

V

D

G

A

S

A

S

S

-

G

-

K

R

T

F

I

D

D

V

V

N

V

D

N

P

P

A

A

T

T

G

G

L

K

K

P

L

I

A

G

D

R

V

V

A

A

N

H

L

A

G

G

P

I

A

A

D

D

A

L

E

D

A

R

A

A

I

L

A

A

N

Q

A

S

A

G

W

F

G

E

P

A

W

W

K

R

T

K

K

V

T

P

A

A

K

H

E

E

R

R

S

A

I

A

I

T

L

M

R

R

K

K

W

A

F

A

D

A

L

L

L

V

M

R

A

E

N

R

Q

A

D

D

D

A

L

I

G

A

R

Q

I

L

M

M

T

T

A

Q

E

E

Q

Q

G

G

K

K

P

P

L

L

A

T

E

E

A

A

K

R

G

V

E

E

V

V

A

L

Y

S

G

A

A

A

S

D

F

I

V

I

E

E

W

W

F

F

A

A

E

D

E

E

A

G

K

R

R

R

I

V

N

Y

G

G

E

R

T

I

L

V

P

P

Q

P

F

R

D

N

N

L

N

G

R

A

R

Q

L

Q

M

T

V

V

L

V

K

K

Q

E

P

P

I

V

G

G

V

P

C

V

A

A

I

F

T

T

P

P

W

W

N
|
N

F

F

P

P

L

V

A

N

M

Q

I

V

T

V

R

R

K

K

V

L

A

S

P

A

A

A

L

L

A

A

A

T

G

G

C

C

P

S

V

F

V

L

I

V

K
|
K

P

A

A

P

E

E

L

E

T

T

P

P

L

A

T

S

A

P

L

A

A

A

A

L

E

R

L

A

A

F

I

V

R

D

A

A

G

G

I

V

P

P

A

A

G

G

V

V

F

I

N

G

I

L

L

V

P

Y

A

G

D

D

S

P

D

A

N

E

S

-

I

-

A

-

I

I

G

S

K

S

V

Y

L

L

C

I

A

P

S

H

D

P

V

V

V

I

R

R

H

K

I

V

S

T

F

F

T

T

G

G

S

S

T

T

E

P

V

V

G

G

R

K

I

Q

L

L

M
x
A

A

S

Q

L

S
x
A

A
x
G

P

L

T

H

V

M

K

K

K

R

M

A

S

T

L

M

E
|
E

L

L

G

G

N

H

A

A

P

P

F

V

I

I

V

V

F

A

D

E

D

D

A

A

D

D

I

V

D

A

S

L

A

A

V

V

E

K

G

A

A

A

F

G

A

G

S

A

K

K

Y

F

R

R

N

N

A

A

G

G

Q

Q

T

V

C
|
C

V

I

C

S

T

P

N

T

R

R

F

F

Y

L

V

V

Q

H

E

N

G

S

V

I

Y

R

D

D

E

E

F

F

V

T

A

R

K

A

F

L

A

V

A

K

K

H

V

A

K

E

T

G

A

L

K

K

V

V

G

G

N

N

G

G

F

L

E

E

A

E

G

G

V

T

N

T

Q

L

G

G

P

A

L

L

I

A

E

N

E

P

A

R

L

L

T

T

K

A

V

M

Q

A

R

S

H

V

V

I

D

D

D

N

A

A

L

R

A

K

K

V

G

G

G

A

Q

S

V

I

V

E

A

T

G

G

Q

E

R

R

L

I

T

G

A

S

L

-

G

-

S

E

G

G

Q

N

F

F

E

A

P

P

T

T

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V

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I

A

A

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N

A

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P

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E

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A

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K

D

T

K

E

L

Q

E

E

E

A

A

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D

A

A

E

A

A

N

N

N

R

T

L

E

P

F

F

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G

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L

A

A

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Y

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N

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Y

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active site: N151 (= N163), K174 (= K186), E249 (= E264), C283 (= C298), E380 (= E397), E457 (= E474)
binding glycerol: A236 (≠ M251), A239 (≠ S254), G240 (≠ A255), Y454 (≠ L471)

4pxlA Structure of zm aldh2-3 (rf2c) in complex with NAD (see paper)
39% identity, 94% coverage: 22:487/498 of query aligns to 9:474/486 of 4pxlA

query
sites
4pxlA

L

F

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D

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G

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A

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-

N

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T

T

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E

E

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L

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F

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D

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A

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I

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x
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W

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N

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Y

P

P

L

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A

T

M

M

I

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T

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F

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K

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K

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A

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F

A

Y

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A

A

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A

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L

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x
G

D
x
P

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T

S

A

I

-

A

-

I

-

G
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G

K
x
A

V

A

L

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A

A

A

S

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M

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V

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E

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L

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G

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F

D

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Y

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A

A

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K

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L

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Y

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T

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F

G

G

P

P

F

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A

M

P

A

V

L

F

M

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K

F

F

K

K

T

T

E

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E

E

E

A

V

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I

D

Q

A

K

A

A

N

N

T

T

E

R

F

Y

G

G

L

L

A

A

S

A

Y

G

F

I

Y

V

S

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K

D

N

V

I

G

D

R

V

I

A

F

N

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T

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E

R

A

S

L

I

E

R

Y

A

G

G

M

A

V

I

G

W

A

I

N

N

V

C

G

Y

I

F

L

A

A

F

T

D

E

P

H

D

V

A

P

P

F

F

G

G

V

Y

K

K

Q

M

S

S

G

G

L

F

G

G

R

K

E
x
D

G

M

S

G

H

M

H

D

G

A

M

L

D

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Y

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K

active site: N154 (= N163), K177 (= K186), E253 (= E264), C287 (= C298), E384 (= E397), D461 (≠ E474)
binding nicotinamide-adenine-dinucleotide: I150 (= I159), V151 (≠ T160), P152 (= P161), W153 (= W162), N154 (= N163), K177 (= K186), P178 (= P187), A179 (= A188), E180 (= E189), G210 (≠ S219), P211 (≠ D220), G214 (= G226), A215 (≠ K227), F228 (= F240), T229 (= T241), G230 (= G242), S231 (= S243), V234 (= V246), E253 (= E264), L254 (= L265), G255 (= G266), C287 (= C298), Q334 (≠ A345), K337 (= K348), E384 (= E397), F386 (= F399)

O14293 Putative aldehyde dehydrogenase-like protein C9E9.09c; EC 1.2.1.- from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
37% identity, 95% coverage: 15:487/498 of query aligns to 18:491/503 of O14293

query
sites
O14293

T

T

L

Y

L

I

K

Q

T

P

D

V

G

G

L

L

-

F

I

I

N

N

G

N

Q

Q

W

H

V

V

-

D

-

S

V

V

G

H

S

G

R

R

F

V

D

K

V

V

N

Y

D

S

P

P

A

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T

G

E

L

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K

L

L

I

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C

D

E

V
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