SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ac3H11_4531 FitnessBrowser__acidovorax_3H11:Ac3H11_4531 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 18 hits to proteins with known functional sites (download)

Q88H32 Ornithine cyclodeaminase; OCD; EC 4.3.1.12 from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
65% identity, 95% coverage: 9:345/353 of query aligns to 2:338/350 of Q88H32

query
sites
Q88H32

T

T

L

Y

Y

F

L

I

S

D

A

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G

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K

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R

E

D

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G

G

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I

I

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E

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K

Y

Y

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V

N

N

G

G

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H

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N

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T

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A

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D

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D

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D

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C

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E

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R45 (= R52) binding
K69 (= K76) binding
T84 (= T91) binding
R112 (= R119) binding ; binding
AQ 139:140 (= AQ 146:147) binding
D161 (= D168) binding
T202 (= T208) binding
VGGD 225:228 (= VGGD 231:234) binding
D228 (= D234) binding
K232 (= K238) binding
S293 (= S299) binding
V294 (= V300) binding
K331 (= K336) binding

1x7dA Crystal structure analysis of ornithine cyclodeaminase complexed with NAD and ornithine to 1.6 angstroms (see paper)
65% identity, 95% coverage: 9:345/353 of query aligns to 1:337/340 of 1x7dA

query
sites
1x7dA

T

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A

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D

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A
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D

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K

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L

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D

M

M

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R

active site: E55 (= E63), D227 (= D234)
binding nicotinamide-adenine-dinucleotide: T83 (= T91), V84 (= V92), T108 (= T116), R111 (= R119), T112 (= T120), G135 (= G143), G137 (= G145), A138 (= A146), Q139 (= Q147), Y159 (≠ F167), D160 (= D168), T161 (= T169), D162 (= D170), V200 (= V207), T201 (= T208), A202 (= A209), I209 (= I216), V224 (= V231), G225 (= G232), D227 (= D234), K231 (= K238), S292 (= S299), V293 (= V300), G294 (= G301)
binding L-ornithine: R44 (= R52), V53 (= V61), E55 (= E63), M57 (= M65), K68 (= K76), V70 (= V78), N71 (= N79), G72 (= G80), R111 (= R119), D227 (= D234), V293 (= V300), G294 (= G301)

1u7hA Structure and a proposed mechanism for ornithine cyclodeaminase from pseudomonas putida (see paper)
65% identity, 95% coverage: 9:345/353 of query aligns to 1:337/341 of 1u7hA

query
sites
1u7hA

T

T

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Y

Y

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D

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F

F

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D

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Y

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D

M

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R

active site: E55 (= E63), D227 (= D234)
binding nicotinamide-adenine-dinucleotide: T83 (= T91), V84 (= V92), R111 (= R119), T112 (= T120), G135 (= G143), G137 (= G145), A138 (= A146), Q139 (= Q147), Y159 (≠ F167), D160 (= D168), T161 (= T169), D162 (= D170), V200 (= V207), T201 (= T208), A202 (= A209), I209 (= I216), V224 (= V231), G225 (= G232), D227 (= D234), K231 (= K238), S292 (= S299), V293 (= V300), G294 (= G301)

O28608 Alanine dehydrogenase; AlaDH; EC 1.4.1.1 from Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16) (see paper)
33% identity, 72% coverage: 75:328/353 of query aligns to 64:319/322 of O28608

query
sites
O28608

F

L

K

K

Y

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V

V

N

N

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S

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H

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G

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N

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x
T

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L

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H

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x
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D

C

G

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P

G

G

K
|
K

T

Q

E

E

L

L

H

D

A

V

D

E

V

I

L

L

R

K

Q

K

A

A

Q

K

V

I

F

V

V

V

E

D

Y

D

A

L

P

E

Q

Q

T

A

R

K

I

H

E

G

G

G

D

E

I

I

Q

N

Q

V

L

A

P

V

A

S

D

K

F

G

A

V

V

I

-

G

-

V

-

E

-

D

-

V

-

H

T

A

E

T

L

I

W

G

E

E

V

V

L

I

A

A

G

G

Q

L

H

K

G

D

G

G

R

R

A

E

S

S

D

D

A

E

V

I

T

T

V

I

F

F

D

D

S
|
S

V

T

G

G

F

L

A

A

L

I

E

Q

D

D

F

V

S

A

A

V

L

A

R

K

F

V

L

V

R

Y

D

E

A

N

A

A

A

L

E

S

L

K

G

N

M

V

G

G

Q

S

P

K

I

I

E

K

L

F

R108 (= R119) binding
TQ 135:136 (≠ AQ 146:147) binding
DVR 157:159 (≠ DTD 168:170) binding
GAD 217:219 (≠ GGD 232:234) binding
K223 (= K238) binding
S290 (= S299) binding

1omoA Alanine dehydrogenase dimer w/bound NAD (archaeal) (see paper)
33% identity, 72% coverage: 75:328/353 of query aligns to 64:319/320 of 1omoA

query
sites
1omoA

F

L

K

K

Y

W

V

V

N

N

G

S

H

H

P

P

K

G

N

N

T

P

R

D

W

K

G

G

L

L

P

P

T

T

V

V

M

M

A

A

F

L

G

M

V

I

L

L

A

N

D

S

V

P

A

E

T

T

G

G

A

F

P

P

L

L

L

A

L

V

S

M

E

D

L

A

T

T

L

Y

T

T

A

S

L

L

R
|
R

T
|
T

A

G

A

A

M

A

S

G

A

G

V

I

A

A

R

K

A

Y

L

L

A

A

R

R

P

K

G

N

S

S

R

S

T

V

M

F

A

G

L

F

I

I

G
|
G

N
x
C

G
|
G

A
x
T

Q
|
Q

S

A

E

Y

F

F

Q

Q

A

L

L

E

A

A

F

L

H

R

L

V

L

F

G

D

I

I

D

G

T

E

L

V

R

K

L

A

F
x
Y

D
|
D

T
x
V

D
x
R

P

E

A

K

A

A

T

A

A

K

K

K

L

F

Q

V

A

S

N

Y

L

C

K

E

G

D

T

R

G

G

P

-

R

-

T

I

V

S

A

A

C

S

T

V

S

Q

T

P

A

A

E

E

A

E

V

A

R

S

G

R

A

C

D

D

V

V

V

L

T

V

T

T

V
x
T

T
|
T

A
x
P

D

S

K

R

T

K

N

-

A

-

T

P

I
x
V

L

V

T

K

P

A

D

E

M

W

L

V

A

E

P

E

G

G

M

T

H

H

I

I

N

N

A

A

V
x
I

G
|
G

G

A

D
|
D

C

G

P

P

G

G

K
|
K

T

Q

E

E

L

L

H

D

A

V

D

E

V

I

L

L

R

K

Q

K

A

A

Q

K

V

I

F

V

V

V

E

D

Y

D

A

L

P

E

Q

Q

T

A

R

K

I

H

E

G

G

G

D

E

I

I

Q

N

Q

V

L

A

P

V

A

S

D

K

F

G

A

V

V

I

-

G

-

V

-

E

-

D

-

V

-

H

T

A

E

T

L

I

W

G

E

E

V

V

L

I

A

A

G

G

Q

L

H

K

G

D

G

G

R

R

A

E

S

S

D

D

A

E

V

I

T

T

V

I

F

F

D

D

S
|
S

V
x
T

G
|
G

F

L

A

A

L

I

E

Q

D

D

F

V

S

A

A

V

L

A

R

K

F

V

L

V

R

Y

D

E

A

N

A

A

A

L

E

S

L

K

G

N

M

V

G

G

Q

S

P

K

I

I

E

K

L

F

active site: D219 (= D234)
binding nicotinamide-adenine-dinucleotide: R108 (= R119), T109 (= T120), G132 (= G143), C133 (≠ N144), G134 (= G145), T135 (≠ A146), Q136 (= Q147), Y156 (≠ F167), D157 (= D168), V158 (≠ T169), R159 (≠ D170), T194 (≠ V207), T195 (= T208), P196 (≠ A209), V201 (≠ I216), I216 (≠ V231), G217 (= G232), D219 (= D234), K223 (= K238), S290 (= S299), T291 (≠ V300), G292 (= G301)

Sites not aligning to the query:

active site: 52

5gzlA Cyclodeaminase_pa
35% identity, 90% coverage: 6:323/353 of query aligns to 4:329/357 of 5gzlA

query
sites
5gzlA

H

H

F

M

S

E

T

T

L

W

Y

V

L

L

S

G

A

R

P

R

D

D

V

V

I

A

A

E

L

V

V

V

Q

A

R

A

K

V

G

G

I

R

E

D

P

E

C

L

L

M

R

R

G

R

I

I

A

I

D

D

Y

R

I

L

-

T

-

G

-

L

-

A

-

E

-

I

-

G

-

R

-

G

-

E

-

R

H

H

A

L

D

S

F

P

L

L

R
|
R

W

-

G
|
G

A
x
G

F

L

D
x
E

K

R

S

S

A

E

R

P

V

V

A

P

S

-

H

-

S

-

R

-

D

-

G

G

V
x
I

I

W

E
|
E

L

W

M

M

P

P

I

H

A

R

D

E

-

P

G

G

E

D

T

H

Y

I

A

T

F

L

K

K

Y

T

V

V

N

G

G
x
Y

H

S

P

P

K

A

N

N

-

P

T

A

R

R

W

F

G

G

L

L

P

P

T
|
T

V
x
I

M

L

A

G

F

T

G

V

V

A

L

R

A

Y

D

D

V

D

A

T

T

T

G

G

A

A

P

L

L

T

L

A

L

L

S

M

E

D

L

G

T

V

L

L

T

L

T
|
T

A

A

L

L

R
|
R

T
|
T

A

G

A

A

M

A

S

S

A

A

V

V

A

A

A

S

R

R

A

L

L

L

A

A

R

R

P

P

G

D

S

S

R

H

T

T

M

L

A

G

L

L

I
|
I

G
|
G

N

T

G
|
G

A
|
A

Q
|
Q

S

A

E

V

F

T

Q

Q

A

L

L

H

A

A

F

L

H

S

H

L

L

V

L

L

G

P

I

L

D

Q

T

R

L

A

R

L

L

V

F

W

D
|
D

T
|
T

D
|
D

P

P

A

A

A
x
H

T

R

A

E

K

S

L

F

Q

A

A

R

N

R

L

A

K

A

G

F

T

T

G

G

P

-

R

V

T

S

V

V

A

E

C

I

T

A

S

E

T

P

A

A

E

R

A

I

V

A

R

A

G

E

A

A

D

D

V

V

V

I

T

S

T

T

V
x
A

T
|
T

A
x
S

D
x
V

K

A

T

V

N

G

A

Q

T

G

I

P

L
x
V

T

L

P

P

D

D

M

T

-

G

L

V

A

R

P

E

G

H

M

L

H

H

I

I

N

N

A

A

V
|
V

G
|
G

G
x
A

D
|
D

C

L

P

V

G

G

K
|
K

T

T

E

E

L

L

H

P

A

L

D

G

V

L

L

L

R

E

Q

R

A

A

Q

F

V

V

F

T

V

A

E

D

Y

H

A

P

P

E

Q

Q

T

A

R

L

I
x
R

E
|
E

G

G

D

E

I

C

Q

Q

L

L

P

S

A

A

D

D

F

R

A

L

V

G

T

P

E

Q

L

L

W

A

E

H

V

L

L

C

A

A

G

D

Q

P

H

A

G

A

R

A

A

G

S

R

D

Q

A

D

A

T

V

L

T

S

V

V

F

F

D

D

S
|
S

V
x
T

G
|
G

F

F

A

A

L

F

E

E

D

D

F

A

S

L

A

A

L

M

R

E

F

V

L

F

R

L

D

E

A

A

E

E

L

R

G

D

M

L

G

G

binding lysine: R53 (= R43), G54 (= G45), G55 (≠ A46), E57 (≠ D48), I65 (≠ V61), E67 (= E63), D240 (= D234), R267 (≠ I261), E268 (= E262)
binding nicotinamide-adenine-dinucleotide: Y85 (≠ G80), T97 (= T91), I98 (≠ V92), T122 (= T116), R125 (= R119), T126 (= T120), I148 (= I142), G149 (= G143), G151 (= G145), A152 (= A146), Q153 (= Q147), D174 (= D168), T175 (= T169), D176 (= D170), H179 (≠ A173), A212 (≠ V207), T213 (= T208), S214 (≠ A209), V215 (≠ D210), V222 (≠ L217), V237 (= V231), G238 (= G232), A239 (≠ G233), D240 (= D234), K244 (= K238), S305 (= S299), T306 (≠ V300), G307 (= G301)

5gziA Cyclodeaminase_pa
35% identity, 90% coverage: 6:323/353 of query aligns to 4:329/354 of 5gziA

query
sites
5gziA

H

H

F

M

S

E

T

T

L

W

Y

V

L

L

S

G

A

R

P

R

D

D

V

V

I

A

A

E

L

V

V

V

Q

A

R

A

K

V

G

G

I

R

E

D

P

E

C

L

L

M

R

R

G

R

I

I

A

I

D

D

Y

R

I

L

-

T

-

G

-

L

-

A

-

E

-

I

-

G

-

R

-

G

-

E

-

R

H

H

A

L

D

S

F

P

L

L

R
|
R

W

-

G

G

A

G

F

L

D

E

K

R

S

S

A

E

R

P

V

V

A

P

S

-

H

-

S

-

R

-

D

-

G

G

V

I

I

W

E
|
E

L

W

M
|
M

P

P

I

H

A

R

D

E

-

P

G

G

E

D

T

H

Y

I

A

T

F

L

K
|
K

Y

T

V

V

N

G

G
x
Y

H

S

P

P

K

A

N

N

-

P

T

A

R

R

W

F

G

G

L

L

P

P

T
|
T

V
x
I

M

L

A

G

F

T

G

V

V

A

L

R

A

Y

D

D

V

D

A

T

T

T

G

G

A

A

P

L

L

T

L

A

L

L

S

M

E

D

L

G

T

V

L

L

T

L

T
|
T

A

A

L

L

R
|
R

T
|
T

A

G

A

A

M

A

S

S

A

A

V

V

A

A

A

S

R

R

A

L

L

L

A

A

R

R

P

P

G

D

S

S

R

H

T

T

M

L

A

G

L

L

I
|
I

G
|
G

N

T

G
|
G

A
|
A

Q
|
Q

S

A

E

V

F

T

Q

Q

A

L

L

H

A

A

F

L

H

S

H

L

L

V

L

L

G

P

I

L

D

Q

T

R

L

A

R

L

L

V

F

W

D
|
D

T
|
T

D
|
D

P

P

A

A

A
x
H

T

R

A

E

K

S

L

F

Q

A

A

R

N

R

L

A

K

A

G

F

T

T

G

G

P

-

R

V

T

S

V

V

A

E

C

I

T

A

S

E

T

P

A

A

E

R

A

I

V

A

R

A

G

E

A

A

D

D

V

V

V

I

T

S

T

T

V
x
A

T
|
T

A
x
S

D
x
V

K

A

T

V

N

G

A

Q

T

G

I

P

L
x
V

T

L

P

P

D

D

M

T

-

G

L

V

A

R

P

E

G

H

M

L

H

H

I

I

N

N

A

A

V
|
V

G
|
G

G
x
A

D
|
D

C

L

P

V

G

G

K
|
K

T

T

E

E

L

L

H

P

A

L

D

G

V

L

L

L

R

E

Q

R

A

A

Q

F

V

V

F

T

V

A

E

D

Y

H

A

P

P

E

Q

Q

T

A

R

L

I

R

E

E

G

G

D

E

I

C

Q

Q

L

L

P

S

A

A

D

D

F

R

A

L

V

G

T

P

E

Q

L

L

W

A

E

H

V

L

L

C

A

A

G

D

Q

P

H

A

G

A

R

A

A

G

S

R

D

Q

A

D

A

T

V

L

T

S

V

V

F

F

D

D

S
|
S

V
x
T

G
|
G

F

F

A

A

L

F

E

E

D

D

F

A

S

L

A

A

L

M

R

E

F

V

L

F

R

L

D

E

A

A

E

E

L

R

G

D

M

L

G

G

binding nicotinamide-adenine-dinucleotide: Y85 (≠ G80), T97 (= T91), I98 (≠ V92), T122 (= T116), R125 (= R119), T126 (= T120), I148 (= I142), G149 (= G143), G151 (= G145), A152 (= A146), Q153 (= Q147), D174 (= D168), T175 (= T169), D176 (= D170), H179 (≠ A173), A212 (≠ V207), T213 (= T208), S214 (≠ A209), V215 (≠ D210), V222 (≠ L217), V237 (= V231), G238 (= G232), A239 (≠ G233), D240 (= D234), K244 (= K238), S305 (= S299), T306 (≠ V300), G307 (= G301)
binding (2S)-piperidine-2-carboxylic acid: R53 (= R43), E67 (= E63), M69 (= M65), K81 (= K76), R125 (= R119), A239 (≠ G233), T306 (≠ V300), G307 (= G301)

5yu4A Structural basis for recognition of l-lysine, l-ornithine, and l-2,4- diamino butyric acid by lysine cyclodeaminase (see paper)
35% identity, 90% coverage: 7:323/353 of query aligns to 1:325/344 of 5yu4A

query
sites
5yu4A

F

M

S

E

T

T

L

W

Y

V

L

L

S

G

A

R

P

R

D

D

V

V

I

A

A

E

L

V

V

V

Q

A

R

A

K

V

G

G

I

R

E

D

P

E

C

L

L

M

R

R

G

R

I

I

A

I

D

D

Y

R

I

L

-

T

-

G

-

L

-

A

-

E

-

I

-

G

-

R

-

G

-

E

-

R

H

H

A

L

D

S

F

P

L

L

R
|
R

W

-

G

G

A

G

F

L

D

E

K

R

S

S

A

E

R

P

V

V

A

P

S

-

H

-

S

-

R

-

D

-

G

G

V

I

I

W

E
|
E

L

W

M
|
M

P

P

I

H

A

R

D

E

-

P

G

G

E

D

T

H

Y

I

A

T

F

L

K
|
K

Y

T

V

V

N

G

G
x
Y

H

S

P

P

K

A

N

N

-

P

T

A

R

R

W

F

G

G

L

L

P

P

T
|
T

V
x
I

M

L

A

G

F

T

G

V

V

A

L

R

A

Y

D

D

V

D

A

T

T

T

G

G

A

A

P

L

L

T

L

A

L

L

S

M

E

D

L

G

T

V

L

L

T

L

T
|
T

A

A

L

L

R
|
R

T
|
T

A

G

A

A

M

A

S

S

A

A

V

V

A

A

A

S

R

R

A

L

L

L

A

A

R

R

P

P

G

D

S

S

R

H

T

T

M

L

A

G

L

L

I
|
I

G
|
G

N

T

G
|
G

A
|
A

Q
|
Q

S

A

E

V

F

T

Q

Q

A

L

L

H

A

A

F

L

H

S

H

L

L

V

L

L

G

P

I

L

D

Q

T

R

L

A

R

L

L

V

F

W

D
|
D

T
|
T

D
|
D

P

P

A

A

A
x
H

T

R

A

E

K

S

L

F

Q

A

A

R

N

R

L

A

K

A

G

F

T

T

G

G

P

V

R

-

T

S

V

V

A

E

C

I

T

A

S

E

T

P

A

A

E

R

A

I

V

A

R

A

G

E

A

A

D

D

V

V

V

I

T

S

T

T

V
x
A

T
|
T

A
x
S

D
x
V

K

A

T

V

N

G

A

Q

T

G

I

P

L
x
V

T

L

P

P

D

D

M

T

-

G

L

V

A

R

P

E

G

H

M

L

H

H

I

I

N

N

A

A

V
|
V

G
|
G

G
x
A

D
|
D

C

L

P

V

G

G

K
|
K

T

T

E

E

L

L

H

P

A

L

D

G

V

L

L

L

R

E

Q

R

A

A

Q

F

V

V

F

T

V

A

E

D

Y

H

A

P

P

E

Q

Q

T

A

R

L

I

R

E

E

G

G

D

E

I

C

Q

Q

L

L

P

S

A

A

D

D

F

R

A

L

V

G

T

P

E

Q

L

L

W

A

E

H

V

L

L

C

A

A

G

D

Q

P

H

A

G

A

R

A

A

G

S

R

D

Q

A

D

A

T

V

L

T

S

V

V

F

F

D

D

S
|
S

V
x
T

G
|
G

F

F

A

A

L

F

E

E

D

D

F

A

S

L

A

A

L

M

R

E

F

V

L

F

R

L

D

E

A

A

E

E

L

R

G

D

M

L

G

G

binding 2,4-diaminobutyric acid: R49 (= R43), E63 (= E63), M65 (= M65), K77 (= K76), R121 (= R119), A235 (≠ G233), D236 (= D234), T302 (≠ V300), G303 (= G301)
binding nicotinamide-adenine-dinucleotide: Y81 (≠ G80), T93 (= T91), I94 (≠ V92), T118 (= T116), R121 (= R119), T122 (= T120), I144 (= I142), G145 (= G143), G147 (= G145), A148 (= A146), Q149 (= Q147), D170 (= D168), T171 (= T169), D172 (= D170), H175 (≠ A173), A208 (≠ V207), T209 (= T208), S210 (≠ A209), V211 (≠ D210), V218 (≠ L217), V233 (= V231), G234 (= G232), A235 (≠ G233), D236 (= D234), K240 (= K238), S301 (= S299), T302 (≠ V300), G303 (= G301)

5yu3A Structural basis for recognition of l-lysine, l-ornithine, and l-2,4- diamino butyric acid by lysine cyclodeaminase (see paper)
35% identity, 90% coverage: 7:323/353 of query aligns to 1:325/344 of 5yu3A

query
sites
5yu3A

F

M

S

E

T

T

L

W

Y

V

L

L

S

G

A

R

P

R

D

D

V

V

I

A

A

E

L

V

V

V

Q

A

R

A

K

V

G

G

I

R

E

D

P

E

C

L

L

M

R

R

G

R

I

I

A

I

D

D

Y

R

I

L

-

T

-

G

-

L

-

A

-

E

-

I

-

G

-

R

-

G

-

E

-

R

H

H

A

L

D

S

F

P

L

L

R
|
R

W

-

G

G

A

G

F

L

D

E

K

R

S

S

A

E

R

P

V

V

A

P

S

-

H

-

S

-

R

-

D

-

G

G

V

I

I

W

E
|
E

L

W

M
|
M

P

P

I

H

A

R

D

E

-

P

G

G

E

D

T

H

Y

I

A

T

F

L

K
|
K

Y

T

V
|
V

N

G

G
x
Y

H

S

P

P

K

A

N

N

-

P

T

A

R

R

W

F

G

G

L

L

P

P

T
|
T

V
x
I

M

L

A

G

F

T

G

V

V

A

L

R

A

Y

D

D

V

D

A

T

T

T

G

G

A

A

P

L

L

T

L

A

L

L

S

M

E

D

L

G

T

V

L

L

T

L

T
|
T

A

A

L

L

R
|
R

T
|
T

A

G

A

A

M

A

S

S

A

A

V

V

A

A

A

S

R

R

A

L

L

L

A

A

R

R

P

P

G

D

S

S

R

H

T

T

M

L

A

G

L

L

I
|
I

G
|
G

N

T

G
|
G

A
|
A

Q
|
Q

S

A

E

V

F

T

Q

Q

A

L

L

H

A

A

F

L

H

S

H

L

L

V

L

L

G

P

I

L

D

Q

T

R

L

A

R

L

L

V

F

W

D
|
D

T
|
T

D
|
D

P

P

A

A

A
x
H

T

R

A

E

K

S

L

F

Q

A

A

R

N

R

L

A

K

A

G

F

T

T

G

G

P

V

R

-

T

S

V

V

A

E

C

I

T

A

S

E

T

P

A

A

E

R

A

I

V

A

R

A

G

E

A

A

D

D

V

V

V

I

T

S

T

T

V
x
A

T
|
T

A
x
S

D
x
V

K

A

T

V

N

G

A

Q

T

G

I

P

L
x
V

T

L

P

P

D

D

M

T

-

G

L

V

A

R

P

E

G

H

M

L

H

H

I

I

N

N

A

A

V
|
V

G
|
G

G
x
A

D
|
D

C

L

P

V

G

G

K
|
K

T

T

E

E

L

L

H

P

A

L

D

G

V

L

L

L

R

E

Q

R

A

A

Q

F

V

V

F

T

V

A

E

D

Y

H

A

P

P

E

Q

Q

T

A

R

L

I

R

E

E

G

G

D

E

I

C

Q

Q

L

L

P

S

A

A

D

D

F

R

A

L

V

G

T

P

E

Q

L

L

W

A

E

H

V

L

L

C

A

A

G

D

Q

P

H

A

G

A

R

A

A

G

S

R

D

Q

A

D

A

T

V

L

T

S

V

V

F

F

D

D

S
|
S

V
x
T

G
|
G

F

F

A

A

L

F

E

E

D

D

F

A

S

L

A

A

L

M

R

E

F

V

L

F

R

L

D

E

A

A

E

E

L

R

G

D

M

L

G

G

binding nicotinamide-adenine-dinucleotide: Y81 (≠ G80), T93 (= T91), I94 (≠ V92), T118 (= T116), R121 (= R119), T122 (= T120), I144 (= I142), G145 (= G143), G147 (= G145), A148 (= A146), Q149 (= Q147), D170 (= D168), T171 (= T169), D172 (= D170), H175 (≠ A173), A208 (≠ V207), T209 (= T208), S210 (≠ A209), V211 (≠ D210), V218 (≠ L217), V233 (= V231), G234 (= G232), A235 (≠ G233), D236 (= D234), K240 (= K238), S301 (= S299), T302 (≠ V300), G303 (= G301)
binding proline: R49 (= R43), E63 (= E63), M65 (= M65), K77 (= K76), V79 (= V78), R121 (= R119), A235 (≠ G233), T302 (≠ V300), G303 (= G301)

6t3eB Structure of thermococcus litoralis delta(1)-pyrroline-2-carboxylate reductase in complex with nadh and l-proline (see paper)
32% identity, 78% coverage: 53:328/353 of query aligns to 42:325/325 of 6t3eB

query
sites
6t3eB

V

I

A

E

S

V

H

E

S

K

R

H

D

N

G

G

V

F

I

I

E
x
L

L

Y

M
|
M

P

P

-

S

-

Y

I

L

A

E

D

D

G

S

E

E

T

A

Y

L

A

A

F

V

K
|
K

Y

V

V
|
V

N

S

G
x
L

H

Y

P

P

K

E

N

N

T

T

R

K

W

K

G

G

L

L

P

P

T
x
S

V
|
V

M

L

A

A

F

S

G

I

V

L

L

L

A

N

D

D

V

P

A

K

T

T

G

G

A

A

P

P

L

L

L

A

L

L

S

M

E

E

L

G

T

T

L

F

T

I

T
|
T

A

A

L

M

R
|
R

T
|
T

A

G

A

A

M

A

S

S

A

G

V

V

A

A

A

T

R

K

A

Y

L

L

A

A

R

R

P

K

G

D

S

S

R

K

T

I

M

A

A

G

L

I

I

I

G
|
G

N
x
A

G
|
G

A
x
V

Q
|
Q

S

A

E

R

F

T

Q

Q

A

L

L

W

A

A

F

V

H

C

H

E

L

V

L

R

G

N

I

I

D

E

T

K

L

A

R

L

L

V

F
x
Y

D
|
D

T
x
I

D

N

P

P

A

K

A
x
N

T

A

A

K

K

K

L

F

Q

A

A

E

N

E

L

M

-

S

K

K

G

K

T

L

G

G

P

I

R

E

T

I

V

K

A

T

C

V

T

E

S

S

T

A

A

R

E

E

A

A

V

T

R

E

G

K

A

S

D

D

V

I

V

L

T

-

V

I

T

V

A
|
A

D
x
T

K
x
T

T
x
A

N

R

A

E

T

P

I
x
V

L

V

T

K

P

G

D

G

M

W

L

I

A

R

P

E

G

G

M

T

H

H

I

I

N

N

A

S

V
|
V

G
|
G

G
x
W

D

V

C

G

P

R

G

D

K

A

T

R

E

E

L

L

H

D

A

S

D

E

V

T

L

V

R

R

Q

K

A

S

Q

K

V

L

F

V

V

V

E

D

Y

S

A

K

P

E

Q

G

T

V

R

L

I

N

E

E

-

S

G

G

D

D

I

I

Q

-

Q

I

L

I

P

P

A

M

D

K

F

E

A

G

V

V

T

I

-

D

-

E

-

G

-

H

-

I

-

H

-

A

E

E

L

L

W

A

E

E

V

I

L

V

A

A

G

G

Q

V

H

K

G

K

G

G

R

R

A

E

S

N

D

N

A

R

A

E

V

I

T

T

V

L

F

F

D

K

S
|
S

V
|
V

G
|
G

F

L

A

A

L

I

E

E

D

D

F

A

S

I

A

T

L

A

R

K

F

L

L

A

R

Y

D

E

A

K

A

A

A

L

E

E

L

H

G

G

M

V

G

G

Q

T

P

N

I

V

E

E

L

L

binding 1,4-dihydronicotinamide adenine dinucleotide: L71 (≠ G80), S82 (≠ T91), V83 (= V92), T107 (= T116), R110 (= R119), T111 (= T120), G134 (= G143), A135 (≠ N144), G136 (= G145), V137 (≠ A146), Q138 (= Q147), Y158 (≠ F167), D159 (= D168), I160 (≠ T169), N164 (≠ A173), A199 (= A209), T200 (≠ D210), T201 (≠ K211), A202 (≠ T212), V206 (≠ I216), V221 (= V231), G222 (= G232), W223 (≠ G233), S296 (= S299), V297 (= V300), G298 (= G301)
binding proline: L52 (≠ E63), M54 (= M65), K67 (= K76), V69 (= V78), V83 (= V92), R110 (= R119), W223 (≠ G233), V297 (= V300)

Sites not aligning to the query:

binding proline: 39

4bv9A Crystal structure of the NADPH form of mouse mu-crystallin. (see paper)
33% identity, 66% coverage: 85:316/353 of query aligns to 76:300/303 of 4bv9A

query
sites
4bv9A

T

T

R

F

W

Y

G

E

L

V

P

P

T
x
S

V
x
H

M

Q

A

A

F

S

G

V

V

L

L

L

A

F

D

D

V

P

A

S

T

N

G

G

A

S

P

L

L

L

L

A

L

V

S

M

E

D

L

G

T

N

L

V

T

I

T
|
T

A

A

L

K

R
|
R

T
|
T

A

A

M

V

S

S

A

A

V

I

A

A

A

T

R

K

A

L

L

L

A

K

R

P

P

P

G

G

S

S

R

D

T

V

M

L

A

C

L

I

I

L

G
|
G

N
x
A

G
|
G

A
x
V

Q
|
Q

S

A

E

Y

F

S

Q

H

A

Y

L

E

A

I

F

F

H

T

H

E

L

Q

L

F

G

S

I

F

D

K

T

E

L

V

R

R

L

M

F

W

D
x
N

T
x
R

D
x
T

P

R

A

E

A
x
N

T

A

A

E

K

K

L

F

Q

A

A

S

N

T

L

V

K

Q

G

G

T

D

G

-

P

-

R

-

T

V

V

R

A

V

C

C

T

S

S

S

T

V

A

Q

E

E

A

A

V

V

R

T

G

G

A

A

D

D

V

V

V

I

T

I

T

T

V
|
V

T
|
T

A
x
M

D

-

K

-

T
x
A

N

T

A

E

T

P

I

I

L

L

T

F

P

G

D

E

M

W

L

V

A

K

P

P

G

G

M

A

H

H

I

I

N

N

A

A

V
|
V

G
|
G

G

A

D
x
S

C

R

P

P

G

D

K

W

T

R

E

E

L

L

H

D

A

D

D

E

V

L

L

M

R

R

Q

Q

A

A

Q

V

V

L

F

Y

V

V

E

D

Y

S

A

R

P

E

Q

A

T

A

R

L

I

K

E

E

-

S

G

G

D

D

I

V

Q

L

L

S

P

G

A

A

D

D

F

I

A

-

V

F

T

A

E

E

L

L

W

G

E

E

V

V

L

I

A

S

G

G

Q

A

H

K

G

P

G

A

R

H

A

C

S

E

D

K

A

-

V

T

T

T

V

V

F

F

D

K

S
|
S

V
x
L

G
|
G

F

M

A

A

L

V

E

E

D

D

F

L

S

V

A

A

L

A

R

K

F

L

L

V

R

Y

D

D

A

S

active site: S220 (≠ D234)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: S82 (≠ T91), H83 (≠ V92), T107 (= T116), R110 (= R119), T111 (= T120), G134 (= G143), A135 (≠ N144), G136 (= G145), V137 (≠ A146), Q138 (= Q147), N159 (≠ D168), R160 (≠ T169), T161 (≠ D170), N164 (≠ A173), V195 (= V207), T196 (= T208), M197 (≠ A209), A198 (≠ T212), V217 (= V231), G218 (= G232), S220 (≠ D234), S283 (= S299), L284 (≠ V300), G285 (= G301)
binding pyruvic acid: T107 (= T116), R110 (= R119), L284 (≠ V300), G285 (= G301)

Sites not aligning to the query:

active site: 58
binding pyruvic acid: 45, 60, 73, 75

4bvaA Crystal structure of the NADPH-t3 form of mouse mu-crystallin. (see paper)
33% identity, 62% coverage: 97:316/353 of query aligns to 87:299/303 of 4bvaA

query
sites
4bvaA

V

L

L

L

A

F

D

D

V

P

A

S

T

N

G

G

A

S

P

L

L

L

L

A

L

V

S

M

E

D

L

G

T

N

L

V

T

I

T
|
T

A

A

L

K

R
|
R

T
|
T

A

A

M

V

S

S

A

A

V

I

A

A

A

T

R

K

A

L

L

L

A

K

R

P

P

P

G

G

S

S

R

D

T

V

M

L

A

C

L

I

I

L

G
|
G

N
x
A

G
|
G

A
x
V

Q
|
Q

S

A

E

Y

F

S

Q

H

A

Y

L

E

A

I

F

F

H

T

H

E

L

Q

L

F

G

S

I

F

D

K

T

E

L

V

R

R

L

M

F

W

D
x
N

T
x
R

D
x
T

P

R

A

E

A
x
N

T

A

A

E

K

K

L

F

Q

A

A

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N

T

L

V

K

Q

G

G

T

D

G

-

P

-

R

-

T

V

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R

A

V

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C

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S

S

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A

Q

E

E

A

A

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G

G

A

A

D

D

V

V

V

I

T

I

T

T

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V

T
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T

A
x
M

D

-

K

-

T
x
A

N

T

A

E

T

P

I

I

L

L

T

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P

G

D

E

M

W

L

V

A

K

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P

G

G

M

A

H

H

I

I

N

N

A

A

V
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V

G
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G

G

A

D
x
S

C
x
R

P

P

G

D

K
x
W

T

R

E

E

L

L

H

D

A

D

D

E

V

L

L

M

R

R

Q

Q

A

A

Q

V

V

L

F

Y

V

V

E

D

Y

S

A

R

P

E

Q

A

T

A

R

L

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E
|
E

-

S

G

G

D

D

I

V

Q

L

L

S

P

G

A

A

D

D

F

I

A

-

V

F

T

A

E

E

L

L

W

G

E

E

V

V

L

I

A

S

G

G

Q

A

H

K

G

P

G

A

R

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A

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K

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-

V

T

T

T

V

V

F

F

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K

S
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S

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x
L

G
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G

F

M

A

A

L

V

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E

D

D

F

L

S

V

A

A

L

A

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F

L

L

V

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Y

D

D

A

S

active site: S219 (≠ D234)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T106 (= T116), R109 (= R119), T110 (= T120), G133 (= G143), A134 (≠ N144), G135 (= G145), V136 (≠ A146), Q137 (= Q147), N158 (≠ D168), R159 (≠ T169), T160 (≠ D170), N163 (≠ A173), V194 (= V207), T195 (= T208), M196 (≠ A209), A197 (≠ T212), V216 (= V231), G217 (= G232), S219 (≠ D234), S282 (= S299), L283 (≠ V300), G284 (= G301)
binding 3,5,3'triiodothyronine: S219 (≠ D234), R220 (≠ C235), W223 (≠ K238), E247 (= E262)

Sites not aligning to the query:

active site: 59
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 82
binding 3,5,3'triiodothyronine: 46, 48, 57, 59, 76, 78, 82

Q14894 Ketimine reductase mu-crystallin; NADP-regulated thyroid-hormone-binding protein; EC 1.5.1.25 from Homo sapiens (Human) (see paper)
31% identity, 65% coverage: 89:316/353 of query aligns to 89:309/314 of Q14894

query
sites
Q14894

L

V

P

P

T

S

V

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M

Q

A

A

F

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V

L

L

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G

G

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L

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L

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A

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R

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A

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F

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L

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L

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L

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W

D
x
N

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x
R

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A

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T

A

A

E

K

K

L

F

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-

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R

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V

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C

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A

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E

E

A

A

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V

R

A

G

G

A

A

D

D

V

V

V

I

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I

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T

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T

A

L

D

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F

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G

D

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M

W

L

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P

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H

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I

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A

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Y

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E

Q

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L

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E

E

-

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H

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G

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G

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A

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T

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V

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F

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S

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L

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A

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A

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D

A

S

GAGVQA 143:148 (≠ GNGAQS 143:148) binding
N168 (≠ D168) binding
R169 (≠ T169) binding

2i99A Crystal structure of human mu_crystallin at 2.6 angstrom (see paper)
31% identity, 65% coverage: 89:316/353 of query aligns to 88:308/312 of 2i99A

query
sites
2i99A

L

V

P

P

T
x
S

V
x
H

M

Q

A

A

F

T

G

V

V

L

L

L

A

F

D

E

V

P

A

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N

G

G

A

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L

L

L

L

A

L

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L

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T

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A

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R

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T

A

A

M

V

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S

A

A

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I

A

A

A

T

R

K

A

F

L

L

A

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P

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G

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S

S

R

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T

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M

L

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L

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I

L

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|
G

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x
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G

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Y

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Y

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F

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A

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K

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L

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G

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G

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-

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x
A

N

T

A

E

T

P

I

I

L

L

T

F

P

G

D

E

M

W

L

V

A

K

P

P

G

G

M

A

H

H

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I

N

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A

A

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V

G
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G

G

A