SitesBLAST
Comparing Ac3H11_4821 FitnessBrowser__acidovorax_3H11:Ac3H11_4821 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
2f2aA Structure of tRNA-dependent amidotransferase gatcab complexed with gln (see paper)
28% identity, 95% coverage: 9:452/467 of query aligns to 8:473/485 of 2f2aA
- active site: K79 (= K80), S154 (= S155), S155 (= S156), S173 (≠ T174), T175 (≠ I176), G176 (= G177), G177 (= G178), S178 (= S179), Q181 (≠ L182)
- binding glutamine: G130 (≠ L131), S154 (= S155), D174 (= D175), T175 (≠ I176), G176 (= G177), S178 (= S179), F206 (≠ P203), Y309 (vs. gap), Y310 (vs. gap), R358 (≠ L333), D425 (≠ I403)
2dqnA Structure of tRNA-dependent amidotransferase gatcab complexed with asn (see paper)
28% identity, 95% coverage: 9:452/467 of query aligns to 8:473/485 of 2dqnA
- active site: K79 (= K80), S154 (= S155), S155 (= S156), S173 (≠ T174), T175 (≠ I176), G176 (= G177), G177 (= G178), S178 (= S179), Q181 (≠ L182)
- binding asparagine: M129 (= M130), G130 (≠ L131), T175 (≠ I176), G176 (= G177), S178 (= S179), Y309 (vs. gap), Y310 (vs. gap), R358 (≠ L333), D425 (≠ I403)
3h0mA Structure of tRNA-dependent amidotransferase gatcab from aquifex aeolicus (see paper)
30% identity, 95% coverage: 11:452/467 of query aligns to 9:466/478 of 3h0mA
- active site: K72 (= K80), S147 (= S155), S148 (= S156), S166 (≠ T174), T168 (≠ I176), G169 (= G177), G170 (= G178), S171 (= S179), Q174 (≠ L182)
- binding glutamine: M122 (= M130), G123 (≠ L131), D167 (= D175), T168 (≠ I176), G169 (= G177), G170 (= G178), S171 (= S179), F199 (≠ P203), Y302 (vs. gap), R351 (vs. gap), D418 (≠ P399)
3h0lA Structure of tRNA-dependent amidotransferase gatcab from aquifex aeolicus (see paper)
30% identity, 95% coverage: 11:452/467 of query aligns to 9:466/478 of 3h0lA
- active site: K72 (= K80), S147 (= S155), S148 (= S156), S166 (≠ T174), T168 (≠ I176), G169 (= G177), G170 (= G178), S171 (= S179), Q174 (≠ L182)
- binding asparagine: G123 (≠ L131), S147 (= S155), G169 (= G177), G170 (= G178), S171 (= S179), Y302 (vs. gap), R351 (vs. gap), D418 (≠ P399)
4yjiA The crystal structure of a bacterial aryl acylamidase belonging to the amidase signature (as) enzymes family (see paper)
31% identity, 96% coverage: 4:452/467 of query aligns to 3:473/490 of 4yjiA
- active site: K79 (= K80), S158 (= S155), S159 (= S156), G179 (≠ I176), G180 (= G177), G181 (= G178), A182 (≠ S179)
- binding n-(4-hydroxyphenyl)acetamide (tylenol): L81 (≠ N82), G132 (= G129), S158 (= S155), G179 (≠ I176), G180 (= G177), A182 (≠ S179)
3a1iA Crystal structure of rhodococcus sp. N-771 amidase complexed with benzamide (see paper)
33% identity, 85% coverage: 69:464/467 of query aligns to 84:506/508 of 3a1iA
- active site: K95 (= K80), S170 (= S155), S171 (= S156), G189 (≠ T174), Q191 (≠ I176), G192 (= G177), G193 (= G178), A194 (≠ S179), I197 (≠ L182)
- binding benzamide: F145 (≠ S132), S146 (= S133), G147 (= G134), Q191 (≠ I176), G192 (= G177), G193 (= G178), A194 (≠ S179), W327 (vs. gap)
5h6sC Crystal structure of hydrazidase s179a mutant complexed with a substrate (see paper)
29% identity, 96% coverage: 7:452/467 of query aligns to 5:444/457 of 5h6sC
- active site: K77 (= K80), S152 (= S155), S153 (= S156), L173 (≠ I176), G174 (= G177), G175 (= G178), S176 (= S179)
- binding 4-oxidanylbenzohydrazide: C126 (≠ G129), R128 (≠ L131), W129 (≠ S132), S152 (= S155), L173 (≠ I176), G174 (= G177), S176 (= S179), W306 (= W313), F338 (≠ I336)
Q7XJJ7 Fatty acid amide hydrolase; AtFAAH; N-acylethanolamine amidohydrolase; EC 3.5.1.99 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
28% identity, 94% coverage: 15:452/467 of query aligns to 137:588/607 of Q7XJJ7
- K205 (= K80) mutation to A: Loss of activity.
- SS 281:282 (= SS 155:156) mutation to AA: Loss of activity.
- GGGS 302:305 (≠ IGGS 176:179) binding substrate
- S305 (= S179) mutation to A: Loss of activity.
- R307 (= R181) mutation to A: Loss of activity.
- S360 (≠ P230) mutation to A: No effect.
6diiH Structure of arabidopsis fatty acid amide hydrolase in complex with methyl linolenyl fluorophosphonate (see paper)
28% identity, 94% coverage: 15:452/467 of query aligns to 137:588/616 of 6diiH
- binding methyl-9Z,12Z,15Z-octadecatrienylphosphonofluoridate: G255 (= G129), T258 (≠ S132), S281 (= S155), G302 (≠ I176), G303 (= G177), S305 (= S179), S472 (≠ W339), I532 (≠ D395), M539 (≠ I403)
Sites not aligning to the query:
8ey9B Structure of arabidopsis fatty acid amide hydrolase mutant s305a in complex with 9-hydroxy-10,12-octadecadienoyl-ethanolamide
27% identity, 94% coverage: 15:452/467 of query aligns to 137:588/605 of 8ey9B