SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ac3H11_615 FitnessBrowser__acidovorax_3H11:Ac3H11_615 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 15 hits to proteins with known functional sites (download)

Q9A9Z1 D-xylonolactone lactonase; Xylono-1,5-lactonase; EC 3.1.1.110 from Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus) (see paper)
39% identity, 92% coverage: 21:312/316 of query aligns to 1:284/289 of Q9A9Z1

query
sites
Q9A9Z1

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E17 (= E37) binding
N145 (= N170) binding
D195 (= D222) binding

7pldB Caulobacter crescentus xylonolactonase with (r)-4-hydroxy-2- pyrrolidone (see paper)
39% identity, 92% coverage: 22:312/316 of query aligns to 2:284/289 of 7pldB

query
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7pldB

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binding (R)-4-hydroxy-2-pyrrolidone: L15 (= L35), E17 (= E37), I31 (= I53), A64 (= A86), G66 (= G88), F67 (= F89), P80 (= P102), R98 (= R122), N100 (= N124), E119 (≠ C143), D138 (= D163), N145 (= N170), K180 (= K208), D195 (= D222), W210 (= W237), W210 (= W237)
binding fe (ii) ion: E17 (= E37), N145 (= N170), D195 (= D222)

7plbB Caulobacter crescentus xylonolactonase with d-xylose (see paper)
39% identity, 92% coverage: 22:312/316 of query aligns to 2:284/289 of 7plbB

query
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7plbB

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binding fe (ii) ion: E17 (= E37), N145 (= N170), D195 (= D222)
binding beta-D-xylopyranose: L15 (= L35), E17 (= E37), E89 (≠ Q113), V90 (≠ P114), D92 (≠ P116), R98 (= R122), N100 (= N124), R109 (= R133), D130 (= D154), N145 (= N170), D174 (≠ T202), D195 (= D222)
binding alpha-D-xylopyranose: A64 (= A86), G66 (= G88), F67 (= F89), P80 (= P102)

4gncA Human smp30/gnl-1,5-ag complex (see paper)
33% identity, 88% coverage: 25:302/316 of query aligns to 5:283/298 of 4gncA

query
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4gncA

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binding 1,5-anhydro-D-glucitol: E17 (= E37), R100 (= R122), N102 (= N124), D203 (= D222), T245 (= T264), N253 (≠ D272), Y254 (≠ L273)
binding calcium ion: E17 (= E37), D103 (= D125), N153 (= N170), D203 (= D222)

Sites not aligning to the query:

binding 1,5-anhydro-D-glucitol: 290, 291

Q15493 Regucalcin; RC; Gluconolactonase; GNL; Senescence marker protein 30; SMP-30; EC 3.1.1.17 from Homo sapiens (Human) (see 2 papers)
33% identity, 88% coverage: 25:302/316 of query aligns to 6:284/299 of Q15493

query
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Q15493

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L

P

G

A

R

K

E

K

L

V

H

C

R

R

W

W

D

D

P

S

A

F

T

T

G

K

A

Q

H

V

Q

Q

R

R

W

V

T

T

F

M

D

D

E

A

E

P

I

V

S

S

A

S

I

V

A

A

E

L

R

R

A

-

H

Q

A

S

P

G

G

G

F

Y

I

V

V

A

T

T

L

I

R

G

R

T

G

K

F

F

A

C

L

A

F

L

D

N

P

-

A

W

T

K

D

E

M

Q

A

S

P

A

R

V

Y

V

L

L

H

A

Q

T

P

V

E

D

P

N

D

D

R

K

A

K

G

N

N

N

R

R

F

F

N
|
N

D

D

G

G

K

K

C

V

D

D

A

P

Q

A

G

G

R

R

F

Y

W

F

A

A

G

G

S

T

M

M

D

A

-

E

-

T

-

A

-

P

F

A

A

V

C

L

E

E

A

R

P

H

T

Q

G

G

A

A

L

L

Y

Y

R

S

Y

L

D

F

S

P

D

D

G

H

S

H

C

V

T

K

R

K

H

Y

D

F

D

D

G

Q

F

V

A

D

V

I

T

S

N
|
N

G

G

P

L

T

D

W

W

S

S

G

L

T

D

G

H

Q

K

G

-

A

-

A

I

M

F

F

Y

N

I

A

D

T

S

I

L

E

S

G

Y

N

S

T

V

Y

D

R

A

Y

F

D

D

S

Y

D

D

L

L

A

Q

T

T

G

G

T

Q

V

I

S

S

N

N

K

R

T

R

L

S

W

V

K

Y

H

K

W

L

L

E

P

K

E

E

D

E

G

Q

L

I

P

P

D
|
D

G

G

M

M

T

C

T

I

D

D

A

A

Q

E

G

G

R

K

L

L

W

W

I

V

A

A

H

C

W

Y

G

N

G

G

W

G

C

R

V

V

T

I

C

R

H

L

D

D

P

P

V

V

T

T

A

G

A

K

E

R

L

L

G

Q

R

T

V

V

R

K

L

L

P

P

V

V

S

D

Q

K

V

T

T

T

T

S

C

C

A

C

F

F

G

G

A

K

D

N

L

Y

R

S

T

E

L

M

F

Y

I

V

S

T

S

C

A

A

R

R

V

D

G

G

L

M

T

D

P

P

E

E

Q

G

L

L

A

L

A

R

E

Q

P

P

L

E

A

A

G

G

A

G

L

I

F

F

A

K

V

I

E18 (= E37) binding ; mutation to A: Reduces enzyme activity by about 90%.
N103 (= N124) mutation to A: Reduces enzyme activity by about 95%.
N154 (= N170) binding ; mutation to A: Reduces enzyme activity by about 95%.
D204 (= D222) binding ; mutation to A: Reduces enzyme activity by over 98%.

3g4hA Crystal structure of human senescence marker protein-30 (zinc bound) (see paper)
33% identity, 88% coverage: 25:302/316 of query aligns to 4:282/297 of 3g4hA

query
sites
3g4hA

V

I

R

E

C

C

V

V

I

L

A

P

L

E

G

N

N

C

A

R

L

C

G

G

E
|
E

G

S

V

P

L

V

W

W

S

E

V

E

R

V

E

S

Q

N

A

S

V

L

Y

L

W

F

V

V

D

D

I

I

L

P

G

A

R

K

E

K

L

V

H

C

R

R

W

W

D

D

P

S

A

F

T

T

G

K

A

Q

H

V

Q

Q

R

R

W

V

T

T

F

M

D

D

E

A

E

P

I

V

S

S

A

S

I

V

A

A

E

L

R

R

A

-

H

Q

A

S

P

G

G

G

F

Y

I

V

V

A

T

T

L

I

R

G

R

T

G

K

F

F

A

C

L

A

F

L

D

N

P

-

A

W

T

K

D

E

M

Q

A

S

P

A

R

V

Y

V

L

L

H

A

Q

T

P

V

E

D

P

N

D

D

R

K

A

K

G

N

N

N

R

R

F

F

N

N

D

D

G

G

K

K

C

V

D

D

A

P

Q

A

G

G

R

R

F

Y

W

F

A

A

G

G

S

T

M

M

D

A

-

E

-

T

-

A

-

P

F

A

A

V

C

L

E

E

A

R

P

H

T

Q

G

G

A

A

L

L

Y

Y

R

S

Y

L

D

F

S

P

D

D

G

H

S

H

C

V

T

K

R

K

H

Y

D

F

D

D

G

Q

F

V

A

D

V

I

T

S

N
|
N

G

G

P

L

T

D

W

W

S

S

G

L

T

D

G

H

Q

K

G

-

A

-

A

I

M

F

F

Y

N

I

A

D

T

S

I

L

E

S

G

Y

N

S

T

V

Y

D

R

A

Y

F

D

D

S

Y

D

D

L

L

A

Q

T

T

G

G

T

Q

V

I

S

S

N

N

K

R

T

R

L

S

W

V

K

Y

H

K

W

L

L

E

P

K

E

E

D

E

G

Q

L

I

P

P

D
|
D

G

G

M

M

T

C

T

I

D

D

A

A

Q

E

G

G

R

K

L

L

W

W

I

V

A

A

H

C

W

Y

G

N

G

G

W

G

C

R

V

V

T

I

C

R

H

L

D

D

P

P

V

V

T

T

A

G

A

K

E

R

L

L

G

Q

R

T

V

V

R

K

L

L

P

P

V

V

S

D

Q

K

V

T

T

T

T

S

C

C

A

C

F

F

G

G

A

K

D

N

L

Y

R

S

T

E

L

M

F

Y

I

V

S

T

S

C

A

A

R

R

V

D

G

G

L

M

T

D

P

P

E

E

Q

G

L

L

A

L

A

R

E

Q

P

P

L

E

A

A

G

G

A

G

L

I

F

F

A

K

V

I

binding zinc ion: E16 (= E37), N152 (= N170), D202 (= D222)

4gnaA Mouse smp30/gnl-xylitol complex (see paper)
32% identity, 88% coverage: 25:302/316 of query aligns to 4:282/297 of 4gnaA

query
sites
4gnaA

V

V

R

E

C

C

V

V

I

L

A

R

L

E

G

N

N

Y

A

R

L

C

G

G

E
|
E

G

S

V

P

L

V

W

W

S

E

V

E

R

A

E

S

Q

Q

A

S

V

L

Y

L

W

F

V

V

D

D

I

I

L

P

G

S

R

K

E

I

L

I

H

C

R

R

W

W

D

D

P

T

A

V

T

S

G

N

A

Q

H

V

Q

Q

R

R

W

V

T

A

F

V

D

D

E

A

E

P

I

V

S

S

A

S

I

V

A

A

E

L

R

R

A

-

H

Q

A

L

P

G

G

G

F

Y

I

V

V

A

T

T

L

I

R

G

R

T

G

K

F

F

A

C

L

A

F

L

D

N

P

-

A

W

T

E

D

N

M

Q

A

S

P

V

R

F

Y

V

L

L

H

A

Q

M

P

V

E

D

P

E

D

D

R

K

A

K

G

N

N

N

R
|
R

F

F

N
|
N

D

D

G

G

K

K

C

V

D

D

A

P

Q

A

G

G

R

R

F

Y

W

F

A

A

G

G

S

T

M

M

D

A

-

E

-
x
E

-

T

-

A

-

P

F

A

A

V

C

L

E

E

A

R

P

H

T

Q

G

G

A

S

L

L

Y

Y

R

S

Y

L

D

F

S

P

D

D

G

H

S

S

C

V

T

K

R

K

H

Y

D

F

D

D

G

Q

F

V

A

D

V

I

T

S

N
|
N

G

G

P

L

T

D

W

W

S

S

G

L

T

D

G

H

Q

K

G

-

A

-

A

I

M

F

F

Y

N

I

A

D

T

S

I

L

E

S

G

Y

N

T

T

V

Y

D

R

A

Y

F

D

D

S

Y

D

D

L

L

A

Q

T

T

G

G

T

Q

V

I

S

S

N

N

K

R

T

R

L

I

W

V

K

Y

H

K

W

M

L

E

P

K

E

D

D

E

G

Q

L

I

P

P

D
|
D

G

G

M

M

T

C

T

I

D

D

A

A

Q

E

G

G

R

K

L

L

W

W

I

V

A

A

H

C

W
x
Y

G

N

G

G

W

G

C

R

V

V

T

I

C

R

H

L

D

D

P

P

V

E

T

T

A

G

A

K

E

R

L

L

G

Q

R

T

V

V

R

K

L

L

P

P

V

V

S

D

Q

K

V

T

T

T

T

S

C

C

A

C

F

F

G

G

A

K

D

D

L

Y

R

S

T

E

L

M

F

Y

I

V

S

T

S

C

A

A

R

R

V

D

G

G

L

L

T

N

P

A

E

E

Q

G

L

L

A

L

A

R

E

Q

P

P

L

D

A

A

G

G

A

N

L

I

F

F

A

K

V

I

binding calcium ion: E16 (= E37), N152 (= N170), D202 (= D222)
binding Xylitol: E16 (= E37), R99 (= R122), N101 (= N124), E119 (vs. gap), N152 (= N170), D202 (= D222), Y217 (≠ W237)

4gn9A Mouse smp30/gnl-glucose complex (see paper)
32% identity, 88% coverage: 25:302/316 of query aligns to 4:282/297 of 4gn9A

query
sites
4gn9A

V

V

R

E

C

C

V

V

I

L

A

R

L

E

G

N

N

Y

A

R

L

C

G

G

E
|
E

G

S

V

P

L

V

W

W

S

E

V

E

R

A

E

S

Q

Q

A

S

V

L

Y

L

W

F

V

V

D

D

I

I

L

P

G

S

R

K

E

I

L

I

H

C

R

R

W

W

D

D

P

T

A

V

T

S

G

N

A

Q

H

V

Q

Q

R

R

W

V

T

A

F

V

D

D

E

A

E

P

I

V

S

S

A

S

I

V

A

A

E

L

R

R

A

-

H

Q

A

L

P

G

G
|
G

F

Y

I

V

V

A

T

T

L

I

R

G

R

T

G

K

F

F

A

C

L

A

F

L

D

N

P

-

A
x
W

T
x
E

D

N

M

Q

A

S

P

V

R

F

Y

V

L

L

H

A

Q

M

P

V

E
x
D

P
x
E

D

D

R

K

A

K

G

N

N

N

R
|
R

F

F

N
|
N

D

D

G

G

K

K

C

V

D

D

A

P

Q

A

G

G

R

R

F

Y

W

F

A

A

G

G

S

T

M

M

D

A

-

E

-
x
E

-

T

-

A

-

P

F

A

A

V

C

L

E

E

A

R

P

H

T

Q

G

G

A

S

L

L

Y
|
Y

R

S

Y

L

D

F

S

P

D

D

G

H

S

S

C

V

T

K

R
x
K

H

Y

D

F

D

D

G

Q

F

V

A

D

V

I

T

S

N
|
N

G

G

P

L

T

D

W

W

S

S

G

L

T

D

G

H

Q

K

G

-

A

-

A

I

M

F

F

Y

N

I

A

D

T
x
S

I

L

E

S

G

Y

N

T

T

V

Y

D

R

A

Y

F

D

D

S

Y

D

D

L

L

A

Q

T

T

G

G

T

Q

V

I

S

S

N

N

K

R

T

R

L

I

W

V

K

Y

H

K

W

M

L

E

P

K

E

D

D

E

G

Q

L

I

P

P

D
|
D

G

G

M

M

T

C

T

I

D

D

A

A

Q

E

G

G

R

K

L

L

W

W

I

V

A

A

H

C

W
x
Y

G

N

G

G

W

G

C

R

V

V

T

I

C

R

H

L

D

D

P

P

V

E

T

T

A

G

A

K

E

R

L

L

G

Q

R

T

V

V

R

K

L

L

P

P

V

V

S

D

Q

K

V

T

T

T

T

S

C

C

A

C

F

F

G

G

A

K

D

D

L

Y

R

S

T

E

L

M

F

Y

I

V

S

T

S

C

A

A

R

R

V

D

G

G

L

L

T

N

P

A

E

E

Q

G

L

L

A

L

A

R

E

Q

P

P

L

D

A

A

G

G

A

N

L

I

F

F

A

K

V

I

binding beta-D-glucopyranose: E16 (= E37), G66 (= G88), W80 (≠ A103), E81 (≠ T104), D92 (≠ E115), E93 (≠ P116), R99 (= R122), N101 (= N124), E119 (vs. gap), Y133 (= Y151), K143 (≠ R161), N152 (= N170), S168 (≠ T188), D202 (= D222), Y217 (≠ W237)
binding calcium ion: E16 (= E37), N152 (= N170), D202 (= D222)

4gn8A Mouse smp30/gnl-1,5-ag complex (see paper)
32% identity, 88% coverage: 25:302/316 of query aligns to 4:282/297 of 4gn8A

query
sites
4gn8A

V

V

R

E

C

C

V

V

I

L

A

R

L

E

G

N

N

Y

A

R

L

C

G

G

E
|
E

G

S

V

P

L

V

W

W

S

E

V

E

R

A

E

S

Q

Q

A

S

V

L

Y

L

W

F

V

V

D

D

I

I

L

P

G

S

R

K

E

I

L

I

H

C

R

R

W

W

D

D

P

T

A

V

T

S

G

N

A

Q

H

V

Q

Q

R

R

W

V

T

A

F

V

D

D

E

A

E

P

I

V

S

S

A

S

I

V

A

A

E

L

R

R

A

-

H
x
Q

A

L

P

G

G

G

F

Y

I

V

V

A

T

T

L

I

R

G

R

T

G

K

F

F

A

C

L

A

F

L

D

N

P

-

A

W

T

E

D

N

M

Q

A

S

P

V

R

F

Y

V

L

L

H

A

Q

M

P

V

E

D

P

E

D

D

R

K

A

K

G

N

N

N

R
|
R

F

F

N
|
N

D

D

G

G

K

K

C

V

D

D

A

P

Q
x
A

G
|
G

R

R

F

Y

W

F

A

A

G

G

S

T

M

M

D

A

-

E

-
x
E

-

T

-

A

-
x
P

F

A

A

V

C

L

E

E

A

R

P

H

T

Q

G

G

A

S

L

L

Y

Y

R

S

Y

L

D

F

S

P

D

D

G

H

S

S

C

V

T

K

R

K

H

Y

D

F

D

D

G

Q

F

V

A

D

V

I

T

S

N
|
N

G

G

P

L

T

D

W

W

S

S

G

L

T

D

G

H

Q

K

G

-

A

-

A

I

M

F

F
x
Y

F

Y

N

I

A

D

T

S

I

L

E

S

G

Y

N

T

T

V

Y

D

R
x
A

Y

F

D

D

S

Y

D
|
D

L

L

A
x
Q

T

T

G

G

T

Q

V

I

S

S

N

N

K

R

T
x
R

L

I

W
x
V

K

Y

H

K

W

M

L

E

P

K

E

D

D

E

G

Q

L

I

P

P

D
|
D

G

G

M

M

T

C

T

I

D

D

A

A

Q

E

G

G

R

K

L

L

W

W

I

V

A

A

H

C

W
x
Y

G

N

G

G

W

G

C

R

V

V

T

I

C

R

H

L

D

D

P
|
P

V

E

T

T

A

G

A

K

E

R

L

L

G

Q

R

T

V

V

R

K

L

L

P

P

V

V

S

D

Q

K

V

T

T

T

T

S

C

C

A

C

F

F

G

G

A

K

D

D

L

Y

R

S

T

E

L

M

F

Y

I

V

S

T

S

C

A

A

R

R

V

D

G

G

L

L

T

N

P

A

E

E

Q

G

L

L

A

L

A

R

E

Q

P

P

L

D

A

A

G

G

A

N

L

I

F

F

A

K

V

I

binding 1,5-anhydro-D-glucitol: Q63 (≠ H85), R99 (= R122), N101 (= N124), A108 (≠ Q131), G109 (= G132), E119 (vs. gap), P122 (vs. gap), N152 (= N170), Y164 (≠ F184), A175 (≠ R195), D179 (= D199), Q181 (≠ A201), R189 (≠ T209), V191 (≠ W211), D202 (= D222), Y217 (≠ W237), P227 (= P247)
binding calcium ion: E16 (= E37), N152 (= N170), D202 (= D222)

Sites not aligning to the query:

binding 1,5-anhydro-D-glucitol: 293, 294

4gn7A Mouse smp30/gnl (see paper)
32% identity, 88% coverage: 25:302/316 of query aligns to 4:282/297 of 4gn7A

query
sites
4gn7A

V

V

R

E

C

C

V

V

I

L

A

R

L

E

G

N

N

Y

A

R

L

C

G

G

E
|
E

G

S

V

P

L

V

W

W

S

E

V

E

R

A

E

S

Q

Q

A

S

V

L

Y

L

W

F

V

V

D

D

I

I

L

P

G

S

R

K

E

I

L

I

H

C

R

R

W

W

D

D

P

T

A

V

T

S

G

N

A

Q

H

V

Q

Q

R

R

W

V

T

A

F

V

D

D

E

A

E

P

I

V

S

S

A

S

I

V

A

A

E

L

R

R

A

-

H

Q

A

L

P

G

G

G

F

Y

I

V

V

A

T

T

L

I

R

G

R

T

G

K

F

F

A

C

L

A

F

L

D

N

P

-

A

W

T

E

D

N

M

Q

A

S

P

V

R

F

Y

V

L

L

H

A

Q

M

P

V

E

D

P

E

D

D

R

K

A

K

G

N

N

N

R

R

F

F

N

N

D

D

G

G

K

K

C

V

D

D

A

P

Q

A

G

G

R

R

F

Y

W

F

A

A

G

G

S

T

M

M

D

A

-

E

-

T

-

A

-

P

F

A

A

V

C

L

E

E

A

R

P

H

T

Q

G

G

A

S

L

L

Y

Y

R

S

Y

L

D

F

S

P

D

D

G

H

S

S

C

V

T

K

R

K

H

Y

D

F

D

D

G

Q

F

V

A

D

V

I

T

S

N
|
N

G

G

P

L

T

D

W

W

S

S

G

L

T

D

G

H

Q

K

G

-

A

-

A

I

M

F

F

Y

N

I

A

D

T

S

I

L

E

S

G

Y

N

T

T

V

Y

D

R

A

Y

F

D

D

S

Y

D

D

L

L

A

Q

T

T

G

G

T

Q

V

I

S

S

N

N

K

R

T

R

L

I

W

V

K

Y

H

K

W

M

L

E

P

K

E

D

D

E

G

Q

L

I

P

P

D
|
D

G

G

M

M

T

C

T

I

D

D

A

A

Q

E

G

G

R

K

L

L

W

W

I

V

A

A

H

C

W

Y

G

N

G

G

W

G

C

R

V

V

T

I

C

R

H

L

D

D

P

P

V

E

T

T

A

G

A

K

E

R

L

L

G

Q

R

T

V

V

R

K

L

L

P

P

V

V

S

D

Q

K

V

T

T

T

T

S

C

C

A

C

F

F

G

G

A

K

D

D

L

Y

R

S

T

E

L

M

F

Y

I

V

S

T

S

C

A

A

R

R

V

D

G

G

L

L

T

N

P

A

E

E

Q

G

L

L

A

L

A

R

E

Q

P

P

L

D

A

A

G

G

A

N

L

I

F

F

A

K

V

I

binding calcium ion: E16 (= E37), N152 (= N170), D202 (= D222)

5gx1A Luciferin-regenerating enzyme collected with serial synchrotron rotational crystallography with accumulated dose of 1.1 mgy (1st measurement) (see paper)
31% identity, 88% coverage: 24:302/316 of query aligns to 3:292/307 of 5gx1A

query
sites
5gx1A

A

V

V

V

R

E

C

K

V

I

I

A

A

E

L

L

G

G

N

K

-

Y

A

T

L

V

G

G

E
|
E

G

G

V

P

L

H

W

W

S

D

V

H

R

E

E

T

Q

Q

A

T

V

L

Y

Y

W

F

V

V

D

D

I

T

L

V

G

E

R

K

E

T

L

F

H

H

R

K

W

Y

D

V

P

P

A

S

T

Q

G

K

A

K

H

Y

Q

T

R

F

W
x
C

T

K

F

V

D

D

E

K

E

L

I

V

S

S

A

F

I

I

A

I

E

P

R

L

A

A

H

G

A

S

P

P

G

G

-

R

F

F

I

V

V

V

T

S

L

L

R

E

R

R

G

E

F

I

A

A

L

I

F
x
L

D

T

-
x
W

P

D

A

G

T

V

D

S

M

A

A

A

P

P

R

T

Y

S

L

I

H

E

-

A

-

I

-

V

Q

N

P

V

E

E

P

P

D

H

R

I

A

K

G

N

N

N

R

R

F

L

N

N

D

D

G

G

K

K

C

A

D

D

A

P

Q

L

G

G

R

N

F

L

W

W

A

T

G

G

S

T

M

M

D

A

F

I

A

D

C

A

E

G

A

L

P

P

-

I

-

G

-

P

-

V

T

T

G

G

A

S

L

L

Y

Y

R

H

Y

L

D

G

S

A

D

D

G

K

S

K

C

V

T

K

R

M

H

H

D

E

D

S

G

N

F

I

A

A

V

I

T

A

N
|
N

G

G

P

L

T

A

W

W

S

S

G

N

T

D

G

L

Q

K

G

-

A

-

A

K

M

M

F

Y

N

I

A

D

T

S

I

G

E

K

G

R

N

R

T

V

Y

D

R

E

Y

Y

D

D

S

Y

D

D

L

A

A

S

T

T

G

L

T

S

V

I

S

S

N

N

K

Q

-

R

-

P

-

L

-

F

T

T

L

F

W

E

K

K

H

H

W

E

L

V

P

P

E

-

D

-

G

G

L

Y

P

P

D
|
D

G

G

M

Q

T

T

T

I

D

D

A

E

Q

E

G

G

R

N

L

L

W

W

I

V

A

A

H

V

W

F

G

Q

G

G

W

Q

-

R

C

I

V

I

T

K

C

I

H

S

D

T

P

Q

V

Q

T

P

A

E

A

V

E

L

L

L

G

D

R

T

V

V

R

K

L

I

P

P

V

D

S

P

Q

Q

V

V

T

T

T

S

C

V

A

A

F

F

G

G

A

P

D

N

L

L

R

D

T

E

L

L

F

Y

I

V

S

T

S

S

A

A

R

G

V

L

G

Q

L

L

T

D

P

D

E

S

Q

S

L

F

A

D

A

K

E

S

P

L

L

V

A

N

G

G

A

H

L

V

F

Y

A

R

V

V

binding mercury (ii) ion: C51 (≠ W71), L81 (≠ F100), W83 (vs. gap), W83 (vs. gap)
binding magnesium ion: E17 (= E37), N159 (= N170), D211 (= D222)

5d9bA Luciferin-regenerating enzyme solved by siras using xfel (refined against native data) (see paper)
31% identity, 88% coverage: 24:302/316 of query aligns to 3:292/307 of 5d9bA

query
sites
5d9bA

A

V

V

V

R

E

C

K

V

I

I

A

A

E

L

L

G

G

N

K

-

Y

A

T

L

V

G

G

E
|
E

G

G

V

P

L

H

W

W

S

D

V

H

R

E

E

T

Q

Q

A

T

V

L

Y

Y

W

F

V

V

D

D

I

T

L

V

G

E

R

K

E

T

L

F

H

H

R

K

W

Y

D

V

P

P

A

S

T

Q

G

K

A

K

H

Y

Q

T

R

F

W

C

T

K

F

V

D

D

E

K

E

L

I

V

S

S

A

F

I

I

A

I

E

P

R

L

A

A

H

G

A

S

P

P

G

G

-

R

F

F

I

V

V

V

T

S

L

L

R

E

R

R

G

E

F

I

A

A

L

I

F

L

D

T

-

W

P

D

A

G

T

V

D

S

M

A

A

A

P

P

R

T

Y

S

L

I

H

E

-

A

-

I

-

V

Q

N

P

V

E

E

P

P

D

H

R

I

A

K

G

N

N

N

R

R

F

L

N

N

D

D

G

G

K

K

C

A

D

D

A

P

Q

L

G

G

R

N

F

L

W

W

A

T

G

G

S

T

M

M

D

A

F

I

A

D

C

A

E

G

A

L

P

P

-

I

-

G

-

P

-

V

T

T

G

G

A

S

L

L

Y

Y

R

H

Y

L

D

G

S

A

D

D

G

K

S

K

C

V

T

K

R

M

H

H

D

E

D

S

G

N

F

I

A

A

V

I

T

A

N
|
N

G

G

P

L

T

A

W

W

S

S

G

N

T

D

G

L

Q

K

G

-

A

-

A

K

M

M

F

Y

N

I

A

D

T

S

I

G

E

K

G

R

N

R

T

V

Y

D

R

E

Y

Y

D

D

S

Y

D

D

L

A

A

S

T

T

G

L

T

S

V

I

S

S

N

N

K

Q

-

R

-

P

-

L

-

F

T

T

L

F

W

E

K

K

H

H

W

E

L

V

P

P

E

-

D

-

G

G

L

Y

P

P

D
|
D

G

G

M

Q

T

T

T

I

D

D

A

E

Q

E

G

G

R

N

L

L

W

W

I

V

A

A

H

V

W

F

G

Q

G

G

W

Q

-

R

C

I

V

I

T

K

C

I

H

S

D

T

P

Q

V

Q

T

P

A

E

A

V

E

L

L

L

G

D

R

T

V

V

R

K

L

I

P

P

V

D

S

P

Q

Q

V

V

T

T

T

S

C

V

A

A

F

F

G

G

A

P

D

N

L

L

R

D

T

E

L

L

F

Y

I

V

S

T

S

S

A

A

R

G

V

L

G

Q

L

L

T

D

P

D

E

S

Q

S

L

F

A

D

A

K

E

S

P

L

L

V

A

N

G

G

A

H

L

V

F

Y

A

R

V

V

binding magnesium ion: E17 (= E37), N159 (= N170), D211 (= D222)

3o4pA Dfpase at 0.85 angstrom resolution (h atoms included) (see paper)
24% identity, 53% coverage: 115:280/316 of query aligns to 108:286/314 of 3o4pA

query
sites
3o4pA

E

K

P

K

D

D

R

S

A

E

G

G

N

R

R

R

F

M

-

Q

-

G

-

C

N
|
N

D

D

G

C

K

A

C

F

D

D

A

Y

Q

E

G

G

R

N

F

L

W

W

-

I

-

T

-

A

-

P

A

A

G

G

S

E

M

V

-

A

-

P

-

A

D

D

F

Y

-

T

-

R

A

S

C

M

E

Q

A

E

P
x
K

T

F

G

G

A

S

L

I

Y

Y

R

C

Y

F

D

T

S

T

D

D

G

G

S

Q

C

M

T

I

R

Q

H

V

D

D

D

T

G
x
A

F
|
F

A

Q

V

F

T

P

N
|
N

G

G

P

I

T

A

W

V

S

R

G

H

T

M

G

N

Q

D

G

G

A

R

-

P

-

Y

A

Q

M

L

F

I

F

V

N

A

A
x
E

T

T

I

P

E

T

G

K

N

K

T

L

Y

W

R

S

Y

Y

D

D

S

I

D

K

L

-

A

G

T

P

G

A

T

K

V

I

S

E

N

N

K
|
K

T

K

L

V

W

W

K

G

H

H

W

-

L

I

P

P

-

G

-

T

E

H

D

E

G

G

L

G

P

A

D
|
D

G

G

M

M

T
x
D

T

F

D

D

A

E

Q

D

G

N

R

N

L

L

W

L

I

V

A

A

H

N

W
|
W

G

G

G

S

W

S

C

H

V

I

T

E

C

V

H

F

D

G

P

P

V

D

T

G

A

G

A

Q

E

P

L

K

G

M

R

R

V

I

R

R

L

C

P

P

V

F

S

E

Q
x
K

V

P

T

S

T

N

C
x
L

A
x
H

F

F

G

-

G

K

A

P

D

Q

L

T

R

K

T

T

L

I

F

F

I

V

S

T

S

E

active site: N120 (= N124), N175 (= N170), D229 (= D222)
binding calcium ion: N120 (= N124), N175 (= N170), D229 (= D222), D232 (≠ T225), L273 (≠ C266), H274 (≠ A267)
binding 1,2-dimethoxyethane: W244 (= W237), K269 (≠ Q262)
binding 2-methoxyethanol: K151 (≠ P146), A170 (≠ G165), F171 (= F166), E194 (≠ A187), K214 (= K208)

Sites not aligning to the query:

active site: 21, 37, 287
binding calcium ion: 21
binding 1,2-dimethoxyethane: 8, 296, 297

Q7SIG4 Diisopropyl-fluorophosphatase; DFPase; EC 3.1.8.2 from Loligo vulgaris (Common European squid) (see 4 papers)
24% identity, 53% coverage: 115:280/316 of query aligns to 108:286/314 of Q7SIG4

query
sites
Q7SIG4

E

K

P

K

D

D

R

S

A

E

G

G

N

R

R

R

F

M

-

Q

-

G

-

C

N
|
N

D
|
D

G

C

K

A

C

F

D

D

A

Y

Q

E

G

G

R

N

F

L

W

W

-

I

-

T

-

A

-

P

A

A

G

G

S

E

M

V

-

A

-

P

-

A

D

D

F
x
Y

-

T

-
x
R

A

S

C
x
M

E

Q

A

E

P

K

T

F

G

G

A

S

L

I

Y

Y

R

C

Y

F

D

T

S

T

D

D

G

G

S

Q

C

M

T

I

R

Q

H

V

D

D

D

T

G

A

F

F

A

Q

V
x
F

T

P

N
|
N

G

G

P

I

T

A

W

V

S

R

G
x
H

T

M

G

N

Q

D

G

G

A

R

-

P

-

Y

A

Q

M

L

F

I

F

V

N

A

A

E

T
|
T

I

P

E

T

G

K

N

K

T

L

Y

W

R

S

Y

Y

D

D

S

I

D

K

L

-

A

G

T

P

G

A

T

K

V

I

S

E

N

N

K

K

T

K

L

V

W

W

K

G

H
|
H

W

-

L

I

P

P

-

G

-

T

E
x
H

D

E

G

G

L

G

P

A

D
|
D

G

G

M

M

T
x
D

T

F

D

D

A

E

Q

D

G
x
N

R

N

L

L

W

L

I

V

A

A

H

N

W
|
W

G

G

G

S

W

S

C
x
H

V

I

T

E

C

V

H

F

D

G

P

P

V

D

T

G

A

G

A

Q

E

P

L

K

G

M

R

R

V

I

R

R

L

C

P

P

V

F

S

E

Q

K

V

P

T
x
S

T
x
N

C
x
L

A
x
H

F

F

G

-

G

K

A

P

D

Q

L

T

R

K

T

T

L

I

F

F

I

V

S

T

S

E

N120 (= N124) mutation to D: 96% decrease in activity. 100% decrease in activity; when associated with N-229.
D121 (= D125) mutation to F: 100% decrease in activity.
Y144 (≠ F141) mutation to S: 8% increase in activity.
R146 (vs. gap) mutation to S: 45% decrease in activity.
M148 (≠ C143) mutation to A: 26% decrease in activity.
F173 (≠ V168) mutation to A: 84% decrease in activity.; mutation to L: 28% decrease in activity.; mutation to S: 68% decrease in activity.; mutation to V: 46% decrease in activity.; mutation to W: 19% decrease in activity.; mutation to Y: 53% decrease in activity.
N175 (= N170) mutation to D: 98% decrease in activity.
H181 (≠ G176) mutation to N: 20% decrease in activity.
T195 (= T188) mutation to A: 60% decrease in activity.; mutation to L: 11% decrease in activity.; mutation to V: 3% decrease in activity.
H219 (= H213) mutation to N: 3% increase in activity.
H224 (≠ E217) mutation to N: 14% increase in activity.
D229 (= D222) mutation to N: 100% decrease in activity. Loss of calcium 1 binding. 100% decrease in activity; when associated with D-120.
D232 (≠ T225) binding ; mutation to S: 3% increase in activity. 19% decrease in activity; when associated with A-271.
N237 (≠ G230) mutation to S: 4% decrease in activity.
W244 (= W237) mutation to F: 44% decrease in activity.; mutation to H: 27% decrease in activity.; mutation to L: 62% decrease in activity.; mutation to Y: No effect on activity.
H248 (≠ C241) mutation to N: 4% increase in activity.
S271 (≠ T264) mutation to A: 30% increase in activity. 19% decrease in activity; when associated with S-232.
N272 (≠ T265) mutation to F: 100% decrease in activity.
L273 (≠ C266) binding
H274 (≠ A267) binding ; mutation to N: 85% decrease in activity.

Sites not aligning to the query:

21 E→Q: 100% decrease in activity. Loss of calcium 1 binding.
37 E→Q: 50% decrease in activity.
77 Q→F: 100% decrease in activity.; Q→W: No effect on activity.; Q→Y: 6% increase in activity.
287 active site, Proton acceptor; H→A: 90% decrease in activity.; H→F: 36% decrease in activity.; H→L: 21% decrease in activity.; H→N: 97% decrease in activity.; H→Q: 54% decrease in activity.; H→W: 44% decrease in activity.; H→Y: 57% decrease in activity.
304 Q→F: 50% decrease in activity.; Q→W: 3% decrease in activity.
314 F→A: 3% increase in activity.

2gvvA Structure of diisopropyl fluorophosphatase (dfpase) in complex with dicyclopentylphosphoroamidate (dcppa) (see paper)
24% identity, 53% coverage: 115:280/316 of query aligns to 106:284/309 of 2gvvA

query
sites
2gvvA

E

K

P

K

D

D

R

S

A

E

G

G

N

R

R

R

F

M

-

Q

-

G

-

C

N
|
N

D

D

G

C

K

A

C

F

D

D

A

Y

Q

E

G

G

R

N

F

L

W

W

-

I

-

T

-

A

-

P

A

A

G

G

S

E

M

V

-

A

-

P

-

A

D

D

F

Y

-

T

-

R

A

S

C

M

E

Q

A

E

P

K

T

F

G

G

A

S

L

I

Y

Y

R

C

Y

F

D

T

S

T

D

D

G

G

S

Q

C

M

T

I

R

Q

H

V

D

D

D

T

G

A

F

F

A

Q

V

F

T

P

N
|
N

G

G

P

I

T

A

W

V

S

R

G

H

T

M

G

N

Q

D

G

G

A

R

-

P

-

Y

A

Q

M

L

F

I

F

V

N

A

A

E

T

T

I

P

E

T

G

K

N

K

T

L

Y

W

R

S

Y

Y

D

D

S

I

D

K

L

-

A

G

T

P

G

A

T

K

V

I

S

E

N

N

K

K

T

K

L

V

W

W

K

G

H

H

W

-

L

I

P

P

-

G

-

T

E

H

D

E

G

G

L

G

P

A

D
|
D

G

G

M

M

T
x
D

T

F

D

D

A

E

Q

D

G

N

R

N

L

L

W

L

I

V

A

A

H

N

W
|
W

G

G

G

S

W

S

C

H

V

I

T

E

C

V

H

F

D

G

P

P

V

D

T

G

A

G

A

Q

E

P

L

K

G

M

R

R

V

I

R

R

L

C

P

P

V

F

S

E

Q

K

V

P

T

S

T

N

C
x
L

A
x
H

F

F

G

-

G

K

A

P

D

Q

L

T

R

K

T

T

L

I

F

F

I

V

S

T

S

E

active site: N118 (= N124), N173 (= N170), D227 (= D222)
binding calcium ion: N118 (= N124), N173 (= N170), D227 (= D222), D230 (≠ T225), L271 (≠ C266), H272 (≠ A267)
binding dicyclopentyl phosphoramidate: N118 (= N124), N173 (= N170), D227 (= D222), W242 (= W237)

Sites not aligning to the query:

active site: 19, 35, 285
binding calcium ion: 19
binding dicyclopentyl phosphoramidate: 19, 34

Query Sequence

>Ac3H11_615 FitnessBrowser__acidovorax_3H11:Ac3H11_615
MRPFLDTPFSPPEGAAFSAPVTGAVRCVIALGNALGEGVLWSVREQAVYWVDILGRELHR
WDPATGAHQRWTFDEEISAIAERAHAPGFIVTLRRGFALFDPATDMAPRYLHQPEPDRAG
NRFNDGKCDAQGRFWAGSMDFACEAPTGALYRYDSDGSCTRHDDGFAVTNGPTWSGTGQG
AAMFFNATIEGNTYRYDSDLATGTVSNKTLWKHWLPEDGLPDGMTTDAQGRLWIAHWGGW
CVTCHDPVTAAELGRVRLPVSQVTTCAFGGADLRTLFISSARVGLTPEQLAAEPLAGALF
AVDTDSLGLPAHPFGG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory