SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ac3H11_671 FitnessBrowser__acidovorax_3H11:Ac3H11_671 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

5uhsA Structure of a semisweet d57a mutant (see paper)
47% identity, 88% coverage: 3:80/89 of query aligns to 1:78/81 of 5uhsA

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5uhsA

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binding beta-D-glucopyranose: F41 (= F43), W48 (= W50)

B0SR19 Sugar transporter SemiSWEET from Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris) (see paper)
45% identity, 92% coverage: 3:84/89 of query aligns to 1:82/85 of B0SR19

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B0SR19

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W48 (= W50) mutation to A: Impairs glucose transport.
N64 (= N66) mutation to A: Impairs glucose transport.

P0DMV3 Sugar transporter SemiSWEET from Escherichia coli (strain UMEA 3162-1) (see paper)
42% identity, 64% coverage: 21:77/89 of query aligns to 21:77/89 of P0DMV3

query
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P0DMV3

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P21 (= P21) mutation to A: Strongly decreases sucrose transporter activity.
Q22 (= Q22) mutation to A: Slightly decreases sucrose transporter activity.
M39 (= M39) mutation to A: No effect on sucrose transporter activity.
Y40 (= Y40) mutation to A: Decreases sucrose transporter activity.
W50 (= W50) mutation to A: Increases sucrose transporter activity.; mutation to F: No effect on sucrose transporter activity.
Y53 (= Y53) mutation to F: Increases sucrose transporter activity.
R57 (≠ L57) mutation to A: Increases sucrose transporter activity.
N66 (= N66) mutation to A: Impairs sucrose transporter activity.

Sites not aligning to the query:

19 F→A: Decreases sucrose transporter activity.

Query Sequence

>Ac3H11_671 FitnessBrowser__acidovorax_3H11:Ac3H11_671
MQLPELIGYLAATLTTCSFVPQALQTFRTRDVSGISLGMYSVFTAGVALWLVYGLALAAW
PIVVANAITLALAGTILGMKVRYGVRGAG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory