SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ac3H11_701 FitnessBrowser__acidovorax_3H11:Ac3H11_701 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1cevA Arginase from bacillus caldovelox, native structure at ph 5.6 (see paper)
44% identity, 82% coverage: 61:362/368 of query aligns to 2:299/299 of 1cevA

query
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1cevA

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active site: H99 (= H156), D122 (= D183), H124 (= H185), D126 (= D187), H139 (= H200), D226 (= D289), D228 (= D291), E271 (= E334)
binding manganese (ii) ion: H99 (= H156), D122 (= D183), D122 (= D183), H124 (= H185), D126 (= D187), D226 (= D289), D226 (= D289), D228 (= D291)

P53608 Arginase; EC 3.5.3.1 from Bacillus caldovelox (see paper)
44% identity, 82% coverage: 61:362/368 of query aligns to 2:299/299 of P53608

query
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P53608

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H99 (= H156) binding
D122 (= D183) binding ; binding
H124 (= H185) binding
HGDVN 124:128 (≠ HADFN 185:189) binding
D126 (= D187) binding
SGN 135:137 (= SGN 196:198) binding
D178 (= D242) binding
D226 (= D289) binding ; binding
D228 (= D291) binding
T240 (= T303) binding
E271 (= E334) binding

5cevA Arginase from bacillus caldevelox, l-lysine complex (see paper)
44% identity, 82% coverage: 61:362/368 of query aligns to 1:298/298 of 5cevA

query
sites
5cevA

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active site: H98 (= H156), D121 (= D183), H123 (= H185), D125 (= D187), H138 (= H200), D225 (= D289), D227 (= D291), E270 (= E334)
binding guanidine: M194 (= M259), H195 (≠ R260), D198 (= D263), R248 (= R312), H251 (≠ Q315), L252 (= L316), E255 (= E319)
binding lysine: H123 (= H185), D125 (= D187), N127 (= N189), S134 (= S196), H138 (= H200), G139 (= G201), D177 (= D242), T239 (= T303), E270 (= E334)
binding manganese (ii) ion: H98 (= H156), D121 (= D183), D121 (= D183), H123 (= H185), D125 (= D187), D225 (= D289), D225 (= D289), D227 (= D291)

4cevA Arginase from bacillus caldevelox, l-ornithine complex (see paper)
44% identity, 82% coverage: 61:362/368 of query aligns to 1:298/298 of 4cevA

query
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4cevA

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N

N

P

P

A

I

L

L

D

D

V

E

R

R

N

N

K

K

T

T

A

A

V

S

L

V

A

A

V

V

D

A

L

L

V

M

E

G

S

S

L

L

F

F

G

G

K

E

S

K

T

L

L

M

active site: H98 (= H156), D121 (= D183), H123 (= H185), D125 (= D187), H138 (= H200), D225 (= D289), D227 (= D291), E270 (= E334)
binding guanidine: M194 (= M259), H195 (≠ R260), D198 (= D263), R248 (= R312), H251 (≠ Q315), L252 (= L316), E255 (= E319)
binding manganese (ii) ion: H98 (= H156), D121 (= D183), D121 (= D183), H123 (= H185), D125 (= D187), D225 (= D289), D225 (= D289), D227 (= D291)
binding L-ornithine: H123 (= H185), D125 (= D187), N127 (= N189), P133 (= P195), S134 (= S196), H138 (= H200), G139 (= G201), D177 (= D242)

3cevA Arginase from bacillus caldevelox, complexed with l-arginine (see paper)
44% identity, 82% coverage: 61:362/368 of query aligns to 1:298/298 of 3cevA

query
sites
3cevA

Q

K

P

P

T

I

S

S

L

I

I

I

G

G

A

V

P

P

T

M

D

D

I

L

G

G

A

Q

G

T

A

R

R

R

G

G

A

V

S

D

M

M

G

G

P

P

E

S

A

A

L

M

R

R

V

Y

A

A

G

G

L

V

Q

I

A

E

A

R

L

L

E

E

L

R

H

L

G

H

L

Y

Q

D

V

I

F

E

D

D

R

L

G

G

N

D

L

I

-

P

-

I

G

G

G

K

P

A

S

E

N

R

P

L

W

H

L

E

P

Q

P

G

V

D

N

S

G

R

Y

L

R

R

H

N

L

L

P

K

E

A

V

V

A

A

Q

E

W

A

N

N

R

E

L

K

V

L

Y

A

D

A

A

A

V

V

Y

D

A

Q

E

V

L

V

Q

Q

L

R

G

G

H

R

L

F

P

P

I

L

L

V

L

L

G

G

G

G

D

D

H
|
H

S

S

L

I

G

A

L

I

G

G

S

T

I

L

S

A

A

G

V

V

A

A

R

K

H

H

C

Y

A

-

E

-

S

-

G

-

K

E

K

R

L

L

R

G

V

V

L

I

W

W

L

Y

D
|
D

A

A

H
|
H

A

G

D
|
D

F

V

N
|
N

T

T

S

A

E

E

L

T

T

S

P
|
P

S
|
S

G

G

N

N

V

I

H
|
H

G
|
G

M

M

P

P

V

L

A

A

C

A

L

S

C

L

G

G

F

F

G

G

P

H

A

P

E

A

L

L

T

T

Q

Q

L

I

A

G

A

-

M

-

P

-

G

G

G

Y

G

S

P

P

A

K

L

I

R

K

P

P

E

E

Q

H

I

V

R

V

Q

L

I

I

G

G

I

V

R

R

S

S

V

L

D
|
D

A

E

G

G

E

E

K

K

R

K

F

F

V

I

H

R

E

E

Q

K

G

G

L

I

E

K

V

I

F

Y

D

T

M
|
M

R
x
H

A

E

I

V

D
|
D

E

R

V

L

G

G

M

M

R

T

Q

R

V

V

M

M

E

E

R

E

A

T

L

I

A

A

G

Y

M

L

D

K

A

E

H

R

T

T

H

D

-

G

L

V

H

H

V

L

S

S

F

L

D
|
D

V

L

D
|
D

F

G

L

L

D

D

P

P

D

S

I

D

A

A

P

P

G

G

V

V

G

G

T
|
T

T

P

V

V

P

I

G

G

G

G

P

L

T

T

Y

Y

R
|
R

E

E

A

S

Q
x
H

L
|
L

C

A

M

M

E
|
E

M

M

I

L

A

A

D

E

S

A

G

Q

R

I

L

I

A

T

S

S

L

A

D

E

I

F

V

V

E
|
E

L

V

N

N

P

P

A

I

L

L

D

D

V

E

R

R

N

N

K

K

T

T

A

A

V

S

L

V

A

A

V

V

D

A

L

L

V

M

E

G

S
|
S

L

L

F

F

G

G

K
x
E

S

K

T
x
L

L

M

active site: H98 (= H156), D121 (= D183), H123 (= H185), D125 (= D187), H138 (= H200), D225 (= D289), D227 (= D291), E270 (= E334)
binding arginine: H98 (= H156), H123 (= H185), D125 (= D187), N127 (= N189), P133 (= P195), S134 (= S196), H138 (= H200), G139 (= G201), D177 (= D242), M194 (= M259), H195 (≠ R260), D198 (= D263), D225 (= D289), T239 (= T303), R248 (= R312), H251 (≠ Q315), L252 (= L316), E255 (= E319), E270 (= E334), S291 (= S355), E295 (≠ K359), L297 (≠ T361)
binding manganese (ii) ion: H98 (= H156), D121 (= D183), D125 (= D187), D225 (= D289)

2cevA Arginase from bacillus caldevelox, native structure at ph 8.5 (see paper)
44% identity, 82% coverage: 61:362/368 of query aligns to 1:298/298 of 2cevA

query
sites
2cevA

Q

K

P

P

T

I

S

S

L

I

I

I

G

G

A

V

P

P

T

M

D

D

I

L

G

G

A

Q

G

T

A

R

R

R

G

G

A

V

S

D

M

M

G

G

P

P

E

S

A

A

L

M

R

R

V

Y

A

A

G

G

L

V

Q

I

A

E

A

R

L

L

E

E

L

R

H

L

G

H

L

Y

Q

D

V

I

F

E

D

D

R

L

G

G

N

D

L

I

-

P

-

I

G

G

G

K

P

A

S

E

N

R

P

L

W

H

L

E

P

Q

P

G

V

D

N

S

G

R

Y

L

R

R

H

N

L

L

P

K

E

A

V

V

A

A

Q

E

W

A

N

N

R

E

L

K

V

L

Y

A

D

A

A

A

V

V

Y

D

A

Q

E

V

L

V

Q

Q

L

R

G

G

H

R

L

F

P

P

I

L

L

V

L

L

G

G

G

G

D

D

H
|
H

S

S

L

I

G

A

L

I

G

G

S

T

I

L

S

A

A

G

V

V

A

A

R

K

H

H

C

Y

A

-

E

-

S

-

G

-

K

E

K

R

L

L

R

G

V

V

L

I

W

W

L

Y

D
|
D

A

A

H
|
H

A

G

D
|
D

F

V

N

N

T

T

S

A

E

E

L

T

T

S

P

P

S

S

G

G

N

N

V

I

H
|
H

G

G

M

M

P

P

V

L

A

A

C

A

L

S

C

L

G

G

F

F

G

G

P

H

A

P

E

A

L

L

T

T

Q

Q

L

I

A

G

A

-

M

-

P

-

G

G

G

Y

G

S

P

P

A

K

L

I

R

K

P

P

E

E

Q

H

I

V

R

V

Q

L

I

I

G

G

I

V

R

R

S

S

V

L

D

D

A

E

G

G

E

E

K

K

R

K

F

F

V

I

H

R

E

E

Q

K

G

G

L

I

E

K

V

I

F

Y

D

T

M
|
M

R
x
H

A

E

I

V

D
|
D

E

R

V

L

G

G

M

M

R

T

Q

R

V

V

M

M

E

E

R

E

A

T

L

I

A

A

G

Y

M

L

D

K

A

E

H

R

T

T

H

D

-

G

L

V

H

H

V

L

S

S

F

L

D
|
D

V

L

D
|
D

F

G

L

L

D

D

P

P

D

S

I

D

A

A

P

P

G

G

V

V

G

G

T

T

T

P

V

V

P

I

G

G

G

G

P

L

T

T

Y

Y

R
|
R

E

E

A

S

Q
x
H

L
|
L

C

A

M

M

E
|
E

M

M

I

L

A

A

D

E

S

A

G

Q

R

I

L

I

A

T

S

S

L

A

D

E

I

F

V

V

E
|
E

L

V

N

N

P

P

A

I

L

L

D

D

V

E

R

R

N

N

K

K

T

T

A

A

V

S

L

V

A

A

V

V

D

A

L

L

V

M

E

G

S

S

L

L

F

F

G

G

K

E

S

K

T
x
L

L

M

active site: H98 (= H156), D121 (= D183), H123 (= H185), D125 (= D187), H138 (= H200), D225 (= D289), D227 (= D291), E270 (= E334)
binding guanidine: M194 (= M259), H195 (≠ R260), D198 (= D263), R248 (= R312), H251 (≠ Q315), L252 (= L316), E255 (= E319), L297 (≠ T361)
binding manganese (ii) ion: H98 (= H156), D121 (= D183), D121 (= D183), H123 (= H185), D125 (= D187), D225 (= D289), D225 (= D289), D227 (= D291)

6dktA Crystal structure of arginase from bacillus subtilis
42% identity, 82% coverage: 61:362/368 of query aligns to 1:283/283 of 6dktA

query
sites
6dktA

Q

K

P

T

T

I

S

S

L

V

I

I

G

G

A

M

P

P

T

M

D

D

I

L

G

G

A

Q

G

A

A

R

R

R

G

G

A

V

S

D

M

M

G

G

P

P

E

S

A

A

L

I

R

R

V

Y

A

A

G

H

L

L

Q

I

A

E

A

R

L

L

E

S

L

D

H

M

G

G

L

Y

Q

T

V

V

F

E

D

D

R

L

G

G

N

D

L

I

G

-

G

-

P

-

S

-

N

-

P

-

W

-

L

-

P

-

P

P

V

I

N

N

G

-

Y

-

R

-

H

N

L

L

P

N

E

S

V

V

A

L

Q

A

W

G

N

N

R

E

L

K

V

L

Y

A

D

Q

A

K

V

V

Y

N

A

K

E

V

L

I

Q

E

L

E

G

K

H

K

L

F

P

P

I

L

L

V

L

L

G

G

G

G

D

D

H
|
H

S

S

L

I

G

A

L

I

G

G

S

T

I

L

S

A

A

G

V

T

A

A

R

K

H

H

C

Y

A

-

E

-

S

-

G

-

K

D

K

N

L

L

R

G

V

V

L

I

W
|
W

L

Y

D
|
D

A

A

H
|
H

A

G

D
|
D

F

L

N

N

T

T

S

L

E

E

L

T

T

S

P

P

S

S

G

G

N

N

V

I

H

H

G

G

M

M

P

P

V

L

A

A

C

V

L

S

C

L

G

G

F

I

G

G

P

H

A

E

E

S

L

L

T

V

Q

N

L

L

A

-

A

-

M

-

P

E

G

G

G

Y

G

A

P

P

A

K

L

I

R

K

P

P

E

E

Q

N

I

V

R

V

Q

I

I

I

G

G

I

A

R

R

S

S

V

L

D

D

A

E

G

G

E

E

K

R

R

K

F

Y

V

I

H

K

E

E

Q

S

G

G

L

M

E

K

V

V

F

Y

D

T

M

M

R

H

A

E

I

I

D

D

E

R

V

L

G

G

M

M

R

T

Q

K

V

V

M

I

E

E

R

E

A

T

L

L

A

D

G

Y

M

L

D

S

A

A

H

C

T

D

H

G

L

V

H

H

V

L

S

S

F

L

D
|
D

V

L

D
|
D

F

G

L

L

D

D

P

P

D

N

I

D

A

A

P

P

G

G

V

V

G

G

T

T

T

P

V

V

P

V

G

G

G

G

P

I

T

S

Y

Y

R

R

E

E

A

S

Q

H

L

L

C

A

M

M

E

E

M

M

I

L

A

Y

D

D

S

A

G

G

R

I

L

I

A

T

S

S

L

A

D

E

I

F

V

V

E

E

L

V

N

N

P

P

A

I

L

L

D

D

V

H

R

K

N

N

K

K

T

T

A

G

V

K

L

T

A

A

V

V

D

E

L

L

V

V

E

E

S

S

L

L

F

L

G

G

K

K

S

K

T

L

L

L

binding manganese (ii) ion: H84 (= H156), W105 (= W181), D107 (= D183), D107 (= D183), H109 (= H185), D111 (= D187), D210 (= D289), D210 (= D289), D212 (= D291)

6nfpC 1.7 angstrom resolution crystal structure of arginase from bacillus subtilis subsp. Subtilis str. 168
42% identity, 82% coverage: 61:362/368 of query aligns to 1:290/290 of 6nfpC

query
sites
6nfpC

Q

K

P

T

T

I

S

S

L

V

I

I

G

G

A

M

P

P

T

M

D
|
D

I
x
L

G
|
G

A

Q

G

A

A
x
R

R

R

G

G

A

V

S

D

M

M

G

G

P

P

E

S

A

A

L

I

R

R

V

Y

A

A

G

H

L

L

Q

I

A

E

A

R

L

L

E

S

L

D

H

M

G

G

L

Y

Q

T

V

V

F

E

D

D

R

L

G

G

N

D

L

I

G

-

G

-

P

-

S

-

N

-

P

-

W

-

L

-

P

-

P

P

V

I

N

N

-

R

-

E

-

D

-

E

G

E

Y

L

R

K

H
x
N

L

L

P

N

E

S

V

V

A

L

Q

A

W

G

N

N

R

E

L

K

V

L

Y

A

D

Q

A

K

V

V

Y

N

A

K

E

V

L

I

Q

E

L

E

G

K

H

K

L

F

P

P

I

L

L

V

L

L

G

G

G

G

D

D

H
|
H

S

S

L

I

G

A

L

I

G

G

S

T

I

L

S

A

A

G

V

T

A

A

R

K

H

H

C

Y

A

-

E

-

S

-

G

-

K

D

K

N

L

L

R

G

V

V

L

I

W

W

L

Y

D
|
D

A

A

H
|
H

A

G

D
|
D

F

L

N

N

T

T

S

L

E

E

L

T

T

S

P

P

S

S

G

G

N

N

V

I

H

H

G

G

M

M

P

P

V

L

A

A

C

V

L

S

C

L

G

G

F

I

G

G

P

H

A

E

E

S

L

L

T

V

Q

N

L

L

A

-

A

-

M

-

P

E

G

G

G

Y

G

A

P

P

A

K

L

I

R

K

P

P

E

E

Q

N

I

V

R

V

Q

I

I

I

G

G

I

A

R

R

S

S

V

L

D

D

A

E

G

G

E

E

K

R

R

K

F

Y

V

I

H

K

E

E

Q

S

G

G

L

M

E

K

V

V

F

Y

D

T

M

M

R

H

A

E

I

I

D

D

E

R

V

L

G

G

M

M

R

T

Q

K

V

V

M

I

E

E

R

E

A

T

L

L

A

D

G

Y

M

L

D

S

A

A

H

C

T

D

H

G

L

V

H

H

V

L

S

S

F

L

D
|
D

V

L

D
|
D

F

G

L

L

D

D

P

P

D

N

I

D

A

A

P

P

G

G

V

V

G

G

T

T

T

P

V

V

P

V

G

G

G

G

P

I

T

S

Y

Y

R

R

E

E

A

S

Q

H

L

L

C

A

M

M

E

E

M

M

I

L

A

Y

D

D

S

A

G

G

R

I

L

I

A

T

S

S

L

A

D

E

I

F

V

V

E

E

L

V

N

N

P

P

A

I

L

L

D

D

V

H

R

K

N

N

K

K

T

T

A

G

V

K

L

T

A

A

V

V

D

E

L

L

V

V

E

E

S

S

L

L

F

L

G

G

K

K

S

K

T

L

L

L

binding glycolic acid: D11 (= D71), L12 (≠ I72), G13 (= G73), R16 (≠ A76), N59 (≠ H124)
binding magnesium ion: H91 (= H156), D114 (= D183), D114 (= D183), H116 (= H185), D118 (= D187), D217 (= D289), D217 (= D289), D219 (= D291)

6nfpA 1.7 angstrom resolution crystal structure of arginase from bacillus subtilis subsp. Subtilis str. 168
41% identity, 82% coverage: 61:362/368 of query aligns to 1:292/292 of 6nfpA

query
sites
6nfpA

Q

K

P

T

T

I

S

S

L

V

I

I

G

G

A

M

P

P

T

M

D

D

I

L

G

G

A

Q

G

A

A

R

R

R

G

G

A

V

S

D

M

M

G

G

P

P

E

S

A

A

L

I

R

R

V

Y

A

A

G

H

L

L

Q

I

A

E

A

R

L

L

E

S

L

D

H

M

G

G

L

Y

Q

T

V

V

F

E

D

D

R

L

G

G

N

D

L

I

G

-

G

-

P

-

S

-

N

-

P

-

W

-

L

-

P

-

P

P

V

I

N

N

-

R

-

E

-

K

-

I

-

D

-

E

G

E

Y

L

R

K

H

N

L

L

P

N

E

S

V

V

A

L

Q

A

W

G

N

N

R

E

L

K

V

L

Y

A

D

Q

A

K

V

V

Y

N

A

K

E

V

L

I

Q

E

L

E

G

K

H

K

L

F

P

P

I

L

L

V

L

L

G

G

G

G

D

D

H
|
H

S

S

L

I

G

A

L

I

G

G

S

T

I

L

S

A

A

G

V

T

A

A

R

K

H

H

C

Y

A

D

E

N

S

L

G

G

K

-

K

-

L

-

R

-

V

V

L

I

W

W

L

Y

D
|
D

A

A

H
|
H

A

G

D
|
D

F

L

N

N

T

T

S

L

E

E

L

T

T

S

P

P

S

S

G

G

N

N

V

I

H

H

G

G

M

M

P

P

V

L

A

A

C

V

L

S

C

L

G

G

F

I

G

G

P

H

A

E

E

S

L

L

T

V

Q

N

L

L

A

-

A

-

M

-

P

E

G

G

G

Y

G

A

P

P

A

K

L

I

R

K

P

P

E

E

Q

N

I

V

R

V

Q

I

I

I

G

G

I

A

R

R

S

S

V

L

D

D

A

E

G

G

E

E

K

R

R

K

F

Y

V

I

H

K

E

E

Q

S

G

G

L

M

E

K

V

V

F

Y

D

T

M

M

R

H

A

E

I

I

D

D

E

R

V

L

G

G

M

M

R

T

Q

K

V

V

M

I

E

E

R

E

A

T

L

L

A

D

G

Y

M

L

D

S

A

A

H

C

T

D

H

G

L

V

H

H

V

L

S

S

F

L

D
|
D

V

L

D
|
D

F

G

L

L

D

D

P

P

D

N

I

D

A

A

P

P

G

G

V

V

G

G

T

T

T

P

V

V

P

V

G

G

G

G

P

I

T

S

Y

Y

R

R

E

E

A

S

Q

H

L

L

C

A

M

M

E

E

M

M

I

L

A

Y

D

D

S

A

G

G

R

I

L

I

A

T

S

S

L

A

D

E

I

F

V

V

E

E

L

V

N

N

P

P

A

I

L

L

D

D

V

H

R

K

N

N

K

K

T

T

A

G

V

K

L

T

A

A

V

V

D

E

L

L

V

V

E

E

S

S

L

L

F

L

G

G

K

K

S

K

T

L

L

L

binding magnesium ion: H93 (= H156), D116 (= D183), D116 (= D183), H118 (= H185), D120 (= D187), D219 (= D289), D219 (= D289), D221 (= D291)

6nbkA Crystal structure of arginase from bacillus cereus
42% identity, 82% coverage: 61:360/368 of query aligns to 1:287/289 of 6nbkA

query
sites
6nbkA

Q

K

P

E

T

I

S

S

L

V

I

I

G

G

A

V

P

P

T

M

D

D

I

L

G

G

A

Q

G

M

A

R

R

R

G

G

A

V

S

D

M

M

G

G

P

P

E

S

A

A

L

I

R

R

V

Y

A

A

G

G

L

V

Q

I

A

E

A

R

L

I

E

E

L

E

H

I

G

G

L

Y

Q

D

V

V

F

K

D

D

R

M

G

G

N

D

L

I

G

C

G

I

P

E

S

E

N

N

P

T

W

K

L

L

P

-

P

-

V

-

N

-

G

-

Y

-

R

R

H

N

L

L

P

T

E

Q

V

V

A

A

Q

T

W

V

N

C

R

N

L

E

V

L

Y

A

D

S

A

K

V

V

Y
x
D

A

H

E

I

L

I

Q
x
E

L

E

G

G

H

R

L

F

P

P

I

L

L

V

L

L

G

G

G

G

D

D

H
|
H

S

S

L

I

G

A

L

I

G

G

S

T

I

L

S

A

A

G

V

V

A

A

R

K

H

H

C

Y

A

-

E

-

S

-

G

-

K

K

K

N

L

L

R

G

V

V

L

I

W

W

L

Y

D
|
D

A

A

H
|
H

A

G

D
|
D

F

L

N

N

T

T

S

E

E

E

L

T

T

S

P

P

S

S

G

G

N

N

V

I

H

H

G

G

M

M

P

S

V

L

A

A

C

A

L

S

C

L

G

G

F

Y

G

G

P

H

A

S

E

S

L

L

T

V

Q

D

L

L

A

Y

A

-

M

-

P

-

G

G

G

A

G

Y

P

P

A

K

L

V

R

K

P

K

E

E

Q

N

I

V

R

V

Q

I

I

I

G

G

I

A

R

R

S

A

V

L

D

D

A

E

G

G

E

E

K

K

R

D

F

F

V

I

H

R

E

N

Q

E

G

G

L

I

E

K

V

V

F

F

D

S

M

M

R

H

A

E

I

I

D

D

E

R

V

M

G

G

M

M

R

T

Q

A

V

V

M

M

E

E

R

E

A

T

L

I

A

A

G

Y

M

L

D

S

A

H

H

T

T

D

H

G

L

V

H

H

V

L

S

S

F

L

D
|
D

V

L

D
|
D

F

G

L

L

D

D

P

P

D

H

I

D

A

A

P

P

G

G

V

V

G

G

T

T

T

P

V

V

P

I

G

G

G

G

P

L

T

S

Y

Y

R

R

E

E

A

S

Q

H

L

L

C

A

M

M

E

E

M

M

I

L

A

A

D

E

S

A

G

D

R

I

L

I

A

T

S

S

L

A

D

E

I

F

V

V

E

E

L

V

N

N

P

T

A

I

L

L

D

D

V

E

R

R

N

N

K

R

T

T

A

A

V

T

L

T

A

A

V

V

D

A

L

L

V

M

E

G

S

S

L

L

F

F

G

G

K

E

S

K

binding manganese (ii) ion: D74 (≠ Y140), E78 (≠ Q144), H90 (= H156), D113 (= D183), D113 (= D183), H115 (= H185), D117 (= D187), D216 (= D289), D216 (= D289), D218 (= D291)

2ef5A Crystal structure of the arginase from thermus thermophilus
45% identity, 81% coverage: 61:359/368 of query aligns to 1:270/273 of 2ef5A

query
sites
2ef5A

Q

E

P

R

T

V

S

A

L

V

I

V

G

G

A

V

P
|
P

T

M

D

D

I

L

G

G

A

V

G

-

A

-

R

-

G

-

A

-

S
x
D

M
|
M

G

G

P

P

E
x
S

A

A

L

L

R

R

V

Y

A

A

G

R

L

L

Q

L

A

E

A

Q

L

L

E

E

L

D

H

L

G

G

L

Y

Q

T

V

V

F

E

D

D

R

L

G

G

N

D

L

V

G

-

G

-

P

-

S

-

N

-

P

-

W

-

L

-

P

-

P

P

V

V

N

S

G

-

Y

L

R

A

H

Y

L

L

P

E

E

E

V

I

A

-

Q

R

W

A

N

A

R

A

L

L

V

V

Y

L

D

K

A

E

V

R

Y

L

A

A

E

A

L

L

Q

P

L

E

G

G

H

V

L

F

P

P

I

I

L

V

L

L

G

G

G

G

D

D

H
|
H

S

S

L

L

G

S

L

M

G

G

S

S

I

V

S

A

A

G

V

A

A

A

R

R

H

-

C

-

A

-

E

-

S

-

G

G

K

R

K

R

L

V

R

G

V

V

L

V

W

W

L

V

D
|
D

A

A

H
|
H

A

A

D
|
D

F

F

N

N

T

T

S

P

E

E

L

-

T

T

P

S

S

S

G

G

N

N

V

V

H
|
H

G

G

M

M

P

P

V

L

A

A

C

V

L

L

C

S

G

G

F

L

G

G

P

H

A

P

E

R

L

L

T

T

Q

E

L

V

A

F

A

R

M

-

P

-

G

-

G

-

G

-

P

-

A

A

L

V

R

D

P

P

E

K

Q

D

I

V

R

V

Q

L

I

V

G

G

I

V

R

R

S

S

V

L

D

D

A

P

G

G

E

E

K

K

R

R

F

L

V

L

H

K

E

E

Q

A

G

G

L

V

E

R

V

V

F

Y

D

T

M

M

R

H

A

E

I

V

D

D

E

R

V

L

G

G

M

V

R

A

Q

R

V

I

M

A

E

E

R

E

A

V

L

L

A

K

G

H

M

L

D

Q

A

G

H

-

T

L

H

P

L

L

H

H

V

V

S

S

F

L

D
|
D

V

A

D
|
D

F

V

L

L

D

D

P

P

D

T

I

L

A

A

P

P

G

G

V

V

G

G

T

T

T

P

V

V

P

P

G

G

G

G

P

L

T

T

Y

Y

R

R

E

E

A

A

Q

H

L

L

C

L

M

M

E

E

M

I

I

L

A

A

D

E

S

S

G

G

R

R

L

V

A

Q

S

S

L

L

D

D

I

L

V

V

E
|
E

L

V

N

N

P

P

A

I

L

L

D

D

V

E

R

R

N

N

K

R

T

T

A

A

V

E

L

M

A

L

V

V

D

G

L

L

V

A

E

L

S

S

L

L

F

L

G

G

K

K

active site: H80 (= H156), D102 (= D183), H104 (= H185), D106 (= D187), H118 (= H200), D200 (= D289), D202 (= D291), E245 (= E334)
binding lysine: P9 (= P69), D15 (≠ S80), M16 (= M81), S19 (≠ E84)
binding manganese (ii) ion: H80 (= H156), D102 (= D183), D102 (= D183), H104 (= H185), D106 (= D187), D200 (= D289), D200 (= D289), D202 (= D291)

6dktE Crystal structure of arginase from bacillus subtilis
39% identity, 82% coverage: 61:362/368 of query aligns to 1:268/268 of 6dktE

query
sites
6dktE

Q

K

P

T

T

I

S

S

L

V

I

I

G

G

A

M

P

P

T

M

D

D

I

L

G

G

A

Q

G

A

A

R

R

R

G

G

A

V

S

D

M

M

G

G

P

P

E

S

A

A

L

I

R

R

V

Y

A

A

G

H

L

L

Q

I

A

E

A

R

L

L

E

S

L

D

H

M

G

G

L

Y

Q

T

V

V

F

E

D

D

R

L

G

G

N

D

L

I

G

-

G

-

P

-

S

-

N

-

P

-

W

-

L

-

P

-

P

P

V

I

N

N

G

-

Y

-

R

-

H

-

L

-

P

-

E

S

V

V

A

L

Q

A

W

G

N

N

R

E

L

K

V

L

Y

A

D

Q

A

K

V

V

Y

N

A

K

E

V

L

I

Q

E

L

E

G

K

H

K

L

F

P

P

I

L

L

V

L

L

G

G

G

G

D

D

H
|
H

S

S

L

I

G

A

L

I

G

G

S

T

I

L

S

A

A

G

V

T

A

A

R

K

H

H

C

Y

A

D

E

N

S

L

G

G

K

-

K

-

L

-

R

-

V

V

L

I

W
|
W

L

Y

D
|
D

A

A

H
|
H

A

G

D
|
D

F

L

N

N

T

-

S

-

E

-

L

-

T

-

P

-

S

-

G

-

N

-

V

-

H

-

G

-

M

M

P

P

V

L

A

A

C

V

L

S

C

L

G

G

F

I

G

G

P

H

A

E

E

S

L

L

T

V

Q

N

L

L

A

-

A

-

M

-

P

E

G

G

G

Y

G

A

P

P

A

K

L

I

R

K

P

P

E

E

Q

N

I

V

R

V

Q

I

I

I

G

G

I

A

R

R

S

S

V

L

D

D

A

E

G

G

E

E

K

R

R

K

F

Y

V

I

H

K

E

E

Q

S

G

G

L

M

E

K

V

V

F

Y

D

T

M

M

R

H

A

E

I

I

D

D

E

R

V

L

G

G

M

M

R

T

Q

K

V

V

M

I

E

E

R

E

A

T

L

L

A

D

G

Y

M

L

D

S

A

A

H

C

T

D

H

G

L

V

H

H

V

L

S

S

F

L

D
|
D

V

L

D
|
D

F

G

L

L

D

D

P

P

D

N

I

D

A

A

P

P

G

G

V

V

G

G

T

T

T

P

V

V

P

V

G

G

G

G

P

I

T

S

Y

Y

R

R

E

E

A

S

Q

H

L

L

C

A

M

M

E

E

M

M

I

L

A

Y

D

D

S

A

G

G

R

I

L

I

A

T

S

S

L

A

D

E

I

F

V

V

E

E

L

V

N

N

P

P

A

I

L

L

D

D

V

H

R

K

N

N

K

K

T

T

A

G

V

K

L

T

A

A

V

V

D

E

L

L

V

V

E

E

S

S

L

L

F

L

G

G

K

K

S

K

T

L

L

L

binding manganese (ii) ion: H81 (= H156), W102 (= W181), D104 (= D183), D104 (= D183), H106 (= H185), D108 (= D187), D195 (= D289), D195 (= D289), D197 (= D291)

P78540 Arginase-2, mitochondrial; Arginase II; Kidney-type arginase; Non-hepatic arginase; Type II arginase; EC 3.5.3.1 from Homo sapiens (Human) (see paper)
38% identity, 82% coverage: 58:360/368 of query aligns to 21:326/354 of P78540

query
sites
P78540

R

K

S

S

A

V

Q

H

P

S

T

V

S

A

L

V

I

I

G

G

A

A

P

P

T

F

D

S

I

Q

G

G

A

Q

G

K

A

R

R

K

G

G

A

V

S

E

M

H

G

G

P

P

E

A

A

A

L

I

R

R

V

E

A

A

G

G

L

L

Q

M

A

K

A

R

L

L

E

S

L

S

H

L

G

G

L

C

Q

H

V

L

F

K

D

D

R

F

G

G

N

D

L

L

G

-

G

-

P

-

S

S

N

F

P

T

W

P

L

V

P

P

P

K

V

D

N

D

G

L

Y

Y

R

N

H

N

L

L

-

I

-

V

-

N

-

P

P

R

E

S

V

V

A

G

Q

L

W

A

N

N

R

Q

L

E

V

L

Y

A

D

E

A

V

V

V

Y

S

A

R

E

A

L

V

Q

S

L

D

G

G

H

Y

L

S

P

C

I

V

L

T

L

L

G

G

G

G

D

D

H
|
H

S

S

L

L

G

A

L

I

G

G

S

T

I

I

S

S

A

G

V

H

A

A

R

R

H

H

C

C

A

P

E

D

S

-

G

-

K

-

K

-

L

L

R

C

V

V

L

V

W

W

L

V

D
|
D

A

A

H
|
H

A
|
A

D
|
D

F
x
I

N
|
N

T

T

S

P

E

L

L

T

T

T

P

S

S
|
S

G
|
G

N
|
N

V

L

H

H

G

G

M

Q

P

P

V

V

A

S

C

F

L

L

C

L

G

R

F

E

G

L

P

Q

A

D

E

K

L

V

T

P

Q

Q

L

L

A

P

A

G

M

F

P

S

G

W

G

I

G

K

P

P

A

C

L

I

R

S

P

S

E

A

Q

S

I

I

R

V

Q

Y

I

I

G

G

I

L

R

R

S

D

V

V

D
|
D

A

P

G

P

E

E

K

H

R

F

F

I

V

L

H

K

E

N

Q

Y

G

D

L

I

E

Q

V

Y

F

F

D

S

M

M

R

R

A

D

I

I

D

D

E

R

V

L

G

G

M

I

R

Q

Q

K

V

V

M

M

E

E

R

R

A

T

L

F

A

D

G

L

M

L

D

I

A

G

H

K

T

R

H

Q

-

R

-

P

L

I

H

H

V

L

S

S

F

F

D
|
D

V

I

D
|
D

F

A

L

F

D

D

P

P

D

T

I

L

A

A

P

P

G

A

V

T

G

G

T

T

T

P

V

V

P

V

G

G

G

G

P

L

T

T

Y

Y

R

R

E

E

A

G

Q

M

L

Y

C

I

M

A

E

E

M

E

I

I

A

H

D

N

S

T

G

G

R

L

L

L

A

S

S

A

L

L

D

D

I

L

V

V

E
|
E

L

V

N

N

P

P

A

Q

L

L

D

A

V

T

R

S

N

E

K

E

-

E

-

A

-

K

-

T

T

T

A

A

V

N

L

L

A

A

V

V

D

D

L

V

V

I

E

A

S

S

L

S

F

F

G

G

K

Q

S

T

H120 (= H156) binding
D143 (= D183) binding ; binding
H145 (= H185) binding
HADIN 145:149 (≠ HADFN 185:189) binding
D147 (= D187) binding
SGN 156:158 (= SGN 196:198) binding
D202 (= D242) binding
D251 (= D289) binding ; binding
D253 (= D291) binding
E296 (= E334) binding

6ss2AAA of arginase-2 in complex with the inhibitory human antigen-binding fragment fab c0021158' (see paper)
38% identity, 81% coverage: 63:360/368 of query aligns to 5:305/320 of 6ss2AAA

query
sites
6ss2AAA

T

V

S

A

L

V

I

I

G

G

A

A

P

P

T

F

D

S

I

Q

G

G

A

Q

G

K

A

R

R

K

G

G

A

V

S

E

M

H

G

G

P

P

E

A

A

A

L

I

R

R

V

E

A

A

G

G

L

L

Q

M

A

K

A

R

L

L

E

S

L

S

H

L

G

G

L

C

Q

H

V

L

F

K

D

D

R

F

G

G

N

D

L

L

G

-

G

-

P

-

S

S

N

F

P

T

W

P

L

V

P

P

P

K

V

D

N

D

G

L

Y

Y

R

N

H

N

L

L

-

I

-

V

-

N

-

P

P

R

E

S

V

V

A

G

Q

L

W

A

N

N

R

Q

L

E

V

L

Y

A

D

E

A

V

V

V

Y

S

A

R

E

A

L

V

Q

S

L

D

G

G

H

Y

L

S

P

C

I

V

L

T

L

L

G

G

G

G

D

D

H
|
H

S

S

L

L

G

A

L

I

G

G

S

T

I

I

S

S

A

G

V

H

A

A

R

R

H

H

C

C

A

P

E

D

S

-

G

-

K

-

K

-

L

L

R

C

V

V

L

V

W

W

L

V

D
|
D

A

A

H
|
H

A

A

D
|
D

F

I

N

N

T

T

S

P

E

L

L

T

T

T

P

S

S

S

G

G

N

N

V

L

H

H

G

G

M

Q

P

P

V

V

A

S

C

F

L

L

C

L

G

R

F

E

G

L

P

Q

A

D

E

K

L

V

T

P

Q

Q

L

L

A

P

A

G

M

F

P

S

G

W

G

I

G

K

P

P

A

C

L

I

R

S

P

S

E

A

Q

S

I

I

R

V

Q

Y

I

I

G

G

I

L

R

R

S

D

V

V

D

D

A

P

G

P

E

E

K

H

R

F

F

I

V

L

H

K

E

N

Q

Y

G

D

L

I

E

Q

V

Y

F

F

D

S

M

M

R

R

A

D

I

I

D

D

E

R

V

L

G

G

M

I

R

Q

Q

K

V

V

M

M

E

E

R

R

A

T

L

F

A

D

G

L

M

L

D

I

A

G

H

K

T

R

H

Q

-

R

-

P

L

I

H

H

V

L

S

S

F

F

D
|
D

V

I

D
|
D

F

A

L

F

D

D

P

P

D

T

I

L

A

A

P

P

G

A

V

T

G

G

T

T

T

P

V

V

P

V

G

G

G

G

P

L

T

T

Y

Y

R

R

E

E

A

G

Q

M

L

Y

C

I

M

A

E

E

M

E

I

I

A

H

D

N

S

T

G

G

R

L

L

L

A

S

S

A

L

L

D

D

I

L

V

V

E

E

L

V

N

N

P

P

A

Q

L

L

D

A

V

T

R

S

N

E

K

E

-

E

-

A

-

K

-

T

T

T

A

A

V

N

L

L

A

A

V

V

D

D

L

V

V

I

E

A

S

S

L

S

F

F

G

G

K

Q

S

T

binding manganese (ii) ion: H99 (= H156), D122 (= D183), D122 (= D183), H124 (= H185), D126 (= D187), D230 (= D289), D230 (= D289), D232 (= D291)

6q39A Complex of arginase 2 with example 49 (see paper)
38% identity, 81% coverage: 63:360/368 of query aligns to 3:303/306 of 6q39A

query
sites
6q39A

T

V

S

A

L

V

I

I

G

G

A

A

P

P

T

F

D

S

I

Q

G

G

A

Q

G

K

A

R

R

K

G

G

A

V

S

E

M

H

G

G

P

P

E

A

A

A

L

I

R

R

V

E

A

A

G

G

L

L

Q

M

A

K

A

R

L

L

E

S

L

S

H

L

G

G

L

C

Q

H

V

L

F

K

D

D

R

F

G

G

N

D

L

L

G

-

G

-

P

-

S

S

N

F

P

T

W

P

L

V

P

P

P

K

V

D

N

D

G

L

Y

Y

R

N

H

N

L

L

-

I

-

V

-

N

-

P

P

R

E

S

V

V

A

G

Q

L

W

A

N

N

R

Q