SitesBLAST
Comparing Ac3H11_719 FitnessBrowser__acidovorax_3H11:Ac3H11_719 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
2nwwA Crystal structure of gltph in complex with tboa (see paper)
29% identity, 88% coverage: 15:413/456 of query aligns to 2:407/407 of 2nwwA
6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
31% identity, 89% coverage: 15:420/456 of query aligns to 7:421/425 of 6zgbA
6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
31% identity, 89% coverage: 15:420/456 of query aligns to 6:420/424 of 6zl4A
- binding decyl-beta-d-maltopyranoside: L191 (≠ K198), G195 (≠ I202), R282 (≠ E289)
- binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ S278), S272 (= S279), S273 (= S280), M307 (≠ L313), T310 (= T316), G353 (= G359), A354 (= A360), R394 (= R394), T395 (≠ S395)
Sites not aligning to the query:
5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
31% identity, 89% coverage: 15:420/456 of query aligns to 9:423/427 of 5e9sA
- binding aspartic acid: R274 (≠ S278), S275 (= S279), S276 (= S280), T313 (= T316), G353 (= G356), V354 (= V357), A357 (= A360), G358 (= G361), D394 (≠ S391), R397 (= R394), T398 (≠ S395)
- binding decyl-beta-d-maltopyranoside: L194 (≠ K198), G198 (≠ I202), Y202 (≠ A206)
- binding sodium ion: Y87 (≠ F92), T90 (≠ F95), S91 (= S96), S276 (= S280), G305 (= G308), A306 (≠ Y309), T307 (≠ S310), N309 (= N312), N309 (= N312), M310 (≠ L313), D311 (= D314), S348 (= S351), I349 (≠ K352), G350 (= G353), T351 (≠ A354), N401 (= N398), V402 (≠ F399), D405 (≠ N402)
6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
31% identity, 89% coverage: 15:420/456 of query aligns to 9:423/426 of 6xwnB
6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
28% identity, 89% coverage: 12:416/456 of query aligns to 8:419/419 of 6x15A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: F46 (≠ L48), F46 (≠ L48), P75 (≠ Q77), L91 (≠ F94), F95 (≠ L98), L130 (= L140), I133 (≠ F143), I159 (≠ V169), Y167 (≠ M177), K196 (= K198), G200 (≠ I202), I207 (≠ L209), F210 (= F212), L250 (≠ V252), I262 (≠ C264), M269 (≠ L271), T334 (= T336), V335 (≠ L337), G336 (= G338), T340 (≠ A342), L343 (= L345), M399 (≠ L396)
- binding aspartic acid: S277 (= S279), S278 (= S280), T314 (= T316), G354 (= G356), A358 (= A360), G359 (= G361), D394 (≠ S391), R397 (= R394), T398 (≠ S395)
- binding sodium ion: Y89 (≠ F92), T92 (≠ F95), S93 (= S96), G306 (= G308), T308 (≠ S310), N310 (= N312), N310 (= N312), M311 (≠ L313), D312 (= D314), S349 (= S351), I350 (≠ K352), T352 (≠ A354), N401 (= N398), V402 (≠ F399), D405 (≠ N402)
Sites not aligning to the query:
O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
28% identity, 89% coverage: 12:418/456 of query aligns to 8:421/425 of O59010
- S65 (≠ T67) mutation to V: Strongly decreased chloride conductance.
- R276 (≠ S278) mutation to S: Increased rate of aspartate transport; when associated with R-395.
- RSS 276:278 (≠ SSS 278:280) binding
- M311 (≠ L313) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
- T314 (= T316) binding
- V355 (= V357) binding
- D394 (≠ S391) binding
- M395 (≠ E392) mutation to R: Increased rate of aspartate transport; when associated with S-276.
- R397 (= R394) mutation to A: Strongly decreased affinity for aspartate.
- N401 (= N398) binding
- D405 (≠ N402) mutation to N: Strongly decreased affinity for aspartate.
6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
28% identity, 88% coverage: 12:413/456 of query aligns to 5:413/413 of 6x14A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (= G71), V83 (≠ M89), I157 (≠ L170), Y164 (≠ M177), K193 (= K198), T305 (≠ S310), I306 (≠ F311), I347 (≠ K352)
- binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (≠ L20), M199 (= M204), S275 (= S280), T311 (= T316), G356 (= G361), L384 (= L384), D391 (≠ S391), R394 (= R394)
Sites not aligning to the query:
6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
31% identity, 89% coverage: 15:420/456 of query aligns to 2:412/416 of 6r7rA
- binding d-aspartic acid: R263 (≠ S278), S265 (= S280), M299 (≠ L313), T302 (= T316), T340 (≠ A354), G342 (= G356), V343 (= V357), G347 (= G361), D383 (≠ S391), R386 (= R394), T387 (≠ S395), N390 (= N398)
- binding decyl-beta-d-maltopyranoside: H23 (= H36), V212 (≠ L227), A216 (= A231)
6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
28% identity, 88% coverage: 13:413/456 of query aligns to 1:408/409 of 6bavA
6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
28% identity, 88% coverage: 13:413/456 of query aligns to 1:408/408 of 6bauA
- binding cysteine: S270 (= S280), M303 (≠ L313), T306 (= T316), A345 (= A355), G346 (= G356), V347 (= V357), G351 (= G361), D386 (≠ S391), C389 (≠ R394), T390 (≠ S395), N393 (= N398)
6bmiA Crystal structure of gltph r397c in complex with l-serine (see paper)
26% identity, 88% coverage: 15:413/456 of query aligns to 3:396/396 of 6bmiA
7awmA Structure of the thermostabilized eaat1 cryst mutant in complex with l-asp, three sodium ions and the allosteric inhibitor ucph101 (see paper)
29% identity, 87% coverage: 28:423/456 of query aligns to 34:411/412 of 7awmA
- binding 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile: S88 (≠ T80), G89 (= G82), G92 (≠ F85), A95 (= A88), V96 (≠ M89), Y99 (≠ F92), M163 (≠ V169), F167 (≠ V173), F293 (≠ L303), V297 (≠ T307)
- binding aspartic acid: S268 (= S279), S269 (= S280), T306 (= T316), G346 (= G356), I347 (≠ V357), A350 (= A360), G351 (= G361), D380 (≠ S391), R383 (= R394), T384 (≠ S395)
O35874 Neutral amino acid transporter A; Alanine/serine/cysteine/threonine transporter 1; ASCT-1; Solute carrier family 1 member 4 from Mus musculus (Mouse) (see 2 papers)
27% identity, 96% coverage: 16:452/456 of query aligns to 38:520/532 of O35874
- N201 (≠ E137) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- N206 (≠ G142) modified: carbohydrate, N-linked (GlcNAc...) asparagine
5mjuA Structure of the thermostabilized eaat1 cryst mutant in complex with the competititve inhibitor tfb-tboa and the allosteric inhibitor ucph101 (see paper)
28% identity, 87% coverage: 28:423/456 of query aligns to 26:397/397 of 5mjuA
- binding 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile: L72 (≠ I72), S80 (≠ T80), G81 (= G82), G84 (≠ F85), Y91 (≠ F92), M156 (≠ V169), F160 (≠ V173), F286 (≠ L303), V290 (≠ T307)
- binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I64 (= I64), I148 (= I161), S262 (= S280), S263 (≠ E281), A292 (≠ Y309), T293 (≠ S310), M296 (≠ L313), T299 (= T316), G329 (= G353), A336 (= A360), G337 (= G361), D366 (≠ S391), R369 (= R394), N373 (= N398)
Q10901 Excitatory amino acid transporter; Sodium-dependent glutamate/ aspartate transporter from Caenorhabditis elegans (see paper)
27% identity, 89% coverage: 25:430/456 of query aligns to 26:479/503 of Q10901
- N177 (≠ H136) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- N187 (≠ G142) modified: carbohydrate, N-linked (GlcNAc...) asparagine
8cuaA Human excitatory amino acid transporter 3 (eaat3) with bound potassium in an intermediate outward facing state (see paper)
27% identity, 88% coverage: 24:424/456 of query aligns to 15:415/416 of 8cuaA
8ctcA Human excitatory amino acid transporter 3 (eaat3) with bound glutamate in an intermediate outward facing state (see paper)
28% identity, 85% coverage: 24:411/456 of query aligns to 12:400/406 of 8ctcA
- binding glutamic acid: S268 (= S279), S269 (= S280), M303 (≠ L313), T306 (= T316), G346 (= G356), A350 (= A360), D380 (≠ S391), R383 (= R394)
- binding sodium ion: Y82 (≠ F92), T85 (≠ F95), T86 (≠ S96), S269 (= S280), G298 (= G308), A299 (≠ Y309), T300 (≠ S310), N302 (= N312), N302 (= N312), M303 (≠ L313), D304 (= D314), S341 (= S351), I342 (≠ K352), G343 (= G353), A344 (= A354), N387 (= N398), D391 (≠ N402)
7xr6A Structure of human excitatory amino acid transporter 2 (eaat2) in complex with way-213613 (see paper)
28% identity, 84% coverage: 22:403/456 of query aligns to 17:404/424 of 7xr6A
- binding (2S)-2-azanyl-4-[[4-[2-bromanyl-4,5-bis(fluoranyl)phenoxy]phenyl]amino]-4-oxidanylidene-butanoic acid: S280 (= S279), S281 (= S280), T318 (= T316), G363 (= G361), M367 (≠ L365), V385 (≠ L384), D388 (= D387), R395 (= R394), T396 (≠ S395)
- binding dodecyl beta-D-glucopyranoside: V19 (= V24), I20 (≠ V25), W389 (≠ R388)
- binding cholesterol hemisuccinate: R80 (= R83), R84 (≠ K87), I95 (≠ L98), I252 (= I248)
Sites not aligning to the query:
P43007 Neutral amino acid transporter A; Alanine/serine/cysteine/threonine transporter 1; ASCT-1; Solute carrier family 1 member 4 from Homo sapiens (Human) (see 4 papers)
26% identity, 89% coverage: 49:452/456 of query aligns to 79:520/532 of P43007
- N201 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- N206 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- E256 (≠ T194) to K: in SPATCCM; does not affect localization at the cell surface; decreased uptake of L-serine and L-alanine; Vmax is decreased by at least 50% for both substrates; 3-fold increase of affinity for L-serine; 2-fold increase of affinity for L-alanine; dbSNP:rs201278558
- R457 (≠ E392) to W: in SPATCCM; does not affect localization at the cell surface; loss of uptake of L-serine and L-alanine; dbSNP:rs761533681
Query Sequence
>Ac3H11_719 FitnessBrowser__acidovorax_3H11:Ac3H11_719
MHAISAPTAHPVRLPFYRQLYFQVVLAIVLGVLLGHFEPSYGEALKPLGDAFIKLVKMII
APVIFLTIVTGIAGMSQLSTVGRVFGKAMAYFLFFSTLALVVGLVVANVVQPGAGMNINV
ADLDQSAVKGYVAKSHEMTLTGFALDIIPKTLVSPFVGDNILQVLLVAVLFGVGLAMVGD
AGRPVLNFLDALTTPVFKVVGIVMKAAPLGAFGAMAFTIGKFGLGSLVNLAWLVGSFYIT
SLLFVVVILGFVARLCGFSVFKLCRYLKAELMLVLGTSSSESALPSLMEKMEKAGCSKSV
VGLVVPTGYSFNLDGTNIYMTLAALFIAQATNTELTLGHQVALLLVAMLSSKGAAGVTGA
GFITLAATLAVVPEVPVAGMALILGVDRFMSECRSLTNFIGNAVATVVVSRWENALDHER
LDAALNGHPLPAAVTAAAAPVVPADMPVGAVARTVA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory