SitesBLAST

P19920 Carbon monoxide dehydrogenase medium chain; CO dehydrogenase subunit M; CO-DH M; EC 1.2.5.3 from Afipia carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5) (Oligotropha carboxidovorans) (see 2 papers)
33% identity, 99% coverage: 3:264/265 of query aligns to 5:283/288 of P19920

query
sites
P19920

S

S

F

F

E

D

Y

Y

Q

H

R

R

A

P

T

K

D

S

P

I

K

A

A

D

A

A

A

V

A

A

S

L

L

L

T

T

A

K

-

L

D

G

S

E

D

D

A

A

K

R

F

P

L

L

A
|
A

G
|
G

Q
x
H

S
|
S

L

L

L

I

P

P

T

I

M

M

R

K

L

T

R

R

L

L

A

A

Q

T

P

P

S

E

Q

H

L

L

I

V

D

D

V

L

T

R

R

D

I

I

P

G

A

D

L

L

K

V

S

G

I

I

T

R

V

E

D

E

A

G

K

T

T

D

V

V

T

V

I

I

G

G

A

A

A

M

V

T

C

T

H

Q

A

H

D

A

V

L

A

I

D

G

H

S

A

D

E

F

L

L

R

A

V

K

L

L

P

P

G

I

L

I

A

R

D

E

L

T

A

S

A

L

H

L

I

I

G

A

D

D

R

P

Q

Q

V

I

R

R

A

Y

L

M

G

G

T
|
T

I
|
I

G
|
G

S
x
N

L

A

A

A

N

N

N

G

D

D

P

P

A

G

A

N

C

D

Y

M

P

P

A

A

A

L

A

M

M

Q

G

C

L

L

D

G

A

A

-

A

-

Y

-

E

-

L

T

T

I

G

V

P

T

E

D

G

R

A

R

R

R

I

I

V

A

A

S

A

S

R

D

D

F

Y

V

Q

G

G

M

A

Y

Y

E

F

T

T

A

A

L

I

A

E

P

P

D

G

E

E

L

L

I

L

V

T

A

A

V

I

E

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S

I

P

P

V

V

P

P

E

P

R

T

A

G

-

H

-

G

-

Y

A

A

Y

Y

E

E

K

K

F

L

R

K

N

R

P

K

A

I

S

G

H

D

F

Y

A

A

L

T

V

A

G

A

V

A

F

A

V

V

A

V

K

L

F

T

A

M

S

S

G

G

V

G

R

K

-

C

-

V

-

T

-

A

-

S

V

I

A

G

V

L

T

T

G

N

A

V

A

A

S

N

S

T

V

P

F

L

R

W

V

A

P

E

E

E

L

A

E

G

S

K

A

V

L

L

S

V

A

G

N

T

F

A

T

L

P

D

E

K

A

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A

A

R

L

G

D

V

K

T

A

V

V

S

A

A

L

A

A

D

E

L

A

N

I

T

T

-

A

-

P

-

A

-

S

D

D

M

G

H

R

A

G

S

P

A

A

D

E

Y

Y

R

R

A

T

H

K

L

M

I

A

P

G

V

V

L

M

A

L

A

R

R

R

A

A

V

V

T

E

K

R

A

A

N

K

AGGHS 32:36 (≠ AGGQS 29:33) binding
TIGGN 111:115 (≠ TIGGS 108:112) binding

1n5wC Crystal structure of the cu,mo-co dehydrogenase (codh); oxidized form (see paper)
33% identity, 99% coverage: 3:264/265 of query aligns to 5:283/287 of 1n5wC

query
sites
1n5wC

S

S

F

F

E

D

Y

Y

Q

H

R

R

A

P

T

K

D

S

P

I

K

A

A

D

A

A

A

V

A

A

S

L

L

L

T

T

A

K

-

L

D

G

S

E

D

D

A

A

K

R

F

P

L

L

A

A

G

G

Q
x
H

S

S

L

L

L

I

P

P

T

I

M

M

R

K

L

T

R

R

L

L

A

A

Q

T

P

P

S

E

Q

H

L

L

I

V

D

D

V

L

T

R

R

D

I

I

P

G

A

D

L

L

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G

I

I

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R

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E

D

E

A

G

K

T

T

D

V

V

T

V

I

I

G

G

A

A

A

M

V

T

C

T

H

Q

A

H

D

A

V

L

A

I

D

G

H

S

A

D

E

F

L

L

R

A

V

K

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L

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P

G

I

L

I

A

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D

E

L

T

A

S

A

L

H

L

I
|
I

G
x
A

D

D

R

P

Q

Q

V
x
I

R

R

A

Y

L

M

G

G

T

T

I

I

G

G

S
x
N

L

A

A

A

N

N

N

G

D

D

P

P

A

G

A
x
N

C
x
D

Y

M

P

P

A

A

A

L

A

M

M

Q

G

C

L

L

D

G

A

A

-

A

-

Y

-

E

-

L

T

T

I

G

V

P

T

E

D

G

R

A

R

R

R

I

I

V

A

A

S

A

S

R

D

D

F

Y

V

Q

G

G

M

A

Y

Y

E

F

T

T

A

A

L

I

A

E

P

P

D

G

E

E

L

L

I

L

V

T

A

A

V

I

E

R

S

I

P

P

V

V

P

P

E

P

R

T

A

G

-

H

-

G

-

Y

A

A

Y

Y

E

E

K
|
K

F

L

R

K

N

R

P

K

A

I

S
x
G

H
x
D

F
x
Y

A

A

L

T

V

A

G

A

V

A

F

A

V

V

A

V

K

L

F

T

A

M

S

S

G

G

V

G

R

K

-

C

-

V

-

T

-

A

-

S

V

I

A

G

V

L

T

T

G

N

A

V

A

A

S

N

S

T

V

P

F

L

R

W

V

A

P

E

E

E

L

A

E

G

S

K

A

V

L

L

S

V

A

G

N

T

F

A

T

L

P

D

E

K

A

P

A

A

R

L

G

D

V

K

T

A

V

V

S

A

A

L

A

A

D

E

L

A

N

I

T

T

-

A

-

P

-

A

-

S

D

D

M

G

H

R

A

G

S

P

A

A

D

E

Y

Y

R

R

A

T

H

K

L

M

I

A

P

G

V

V

L

M

A

L

A

R

R

R

A

A

V

V

T

E

K

R

A

A

N

K

binding flavin-adenine dinucleotide: H35 (≠ Q32), I101 (= I98), A102 (≠ G99), I106 (≠ V103), N115 (≠ S112), N123 (≠ A120), D124 (≠ C121), K185 (= K175), G191 (≠ S181), D192 (≠ H182), Y193 (≠ F183)

1t3qC Crystal structure of quinoline 2-oxidoreductase from pseudomonas putida 86 (see paper)
33% identity, 69% coverage: 3:186/265 of query aligns to 5:193/285 of 1t3qC

query
sites
1t3qC

S

A

F

F

E

S

Y

Y

Q

R

R

A

A

P

T

A

D

S

P

L

K

Q

A

E

A

V

A

I

A

Q

S

V

L

L

T

A

A

D

D

D

S

P

D

D

A

A

K

R

F
x
I

L
x
I

A
|
A

G
|
G

Q
|
Q

S
|
S

L
|
L

L

L

P

P

T

L

M

L

R

A

L

F

R

R

L

L

A

V

Q

Y

P

P

S

S

Q

C

L

L

I

V

D

D

V
x
L

T

R

R

N

I

V

P

S

A

E

L

L

K

F

S

E

I

I

T

S

V

Q

D

S

A

A

K

G

T

I

V

L

T

S

I

V

G

G

A

A

A

M

V

V

C

T

H
|
H

A

F

D

R

V

N

A

K

D

T

H

D

A

P

E

T

L

V

R

A

R

K

V

C

L

V

P

P

G

I

L

L

A

P

D

K

L

V

A

L

A

A

H

H

I
x
V

G
x
A

D

H

R

Q

Q

A

V
|
V

R

R

A

N

L

R

G
|
G

T
|
T

I

L

G
|
G

S
|
S

L

L

A
|
A

N
x
H

N

A

D

D

P

A

A

G

A
|
A

C
x
E

Y

M

P

P

A

F

A

L

A

M

M

A

G

T

L

L

D

G

A

A

T

T

I

M

V

Y

T

I

D

A

R

S

-

S

-

A

-

G

-

V

R

R

R

S

I

V

A

S

S

A

S

T

D

D

F

F

F

M

V

K

G

G

M

H

Y

Y

E

F

T

T

A

D

L
|
L

A

E

P

A

D

G

E

E

L
x
V

I
x
L

V

V

A

R

V

V

E

E

S

I

P

P

V

I

P

P

E

A

-

L

R

H

A

W

A

E

Y

F

E

D

K

E

F

Y

R

A

N

R

P

R

A

K

S

G

H

D

F
x
Y

A

A

L

L

V

V

binding flavin-adenine dinucleotide: I29 (≠ F27), I30 (≠ L28), A31 (= A29), G32 (= G30), G33 (= G31), Q34 (= Q32), S35 (= S33), L36 (= L34), L53 (≠ V51), H77 (= H75), V100 (≠ I98), A101 (≠ G99), V105 (= V103), G109 (= G107), T110 (= T108), G112 (= G110), G113 (= G111), S114 (= S112), A116 (= A114), H117 (≠ N115), A122 (= A120), E123 (≠ C121), L160 (= L154), V165 (≠ L159), L166 (≠ I160), Y190 (≠ F183)

5g5gB Escherichia coli periplasmic aldehyde oxidase (see paper)
32% identity, 83% coverage: 1:219/265 of query aligns to 1:276/316 of 5g5gB

query
sites
5g5gB

M

M

Y

K

S

A

F

F

E

T

Y

Y

Q

E

R

R

A

V

T

N

D

T

P

P

K

A

A

E

A

A

A

L

S

S

L

A

T

Q

A

R

D

V

S

P

D

G

A

A

K
|
K

F
|
F

L
x
I

A
|
A

G
|
G

Q
x
T

S
x
N

L
|
L

L

L

P

D

T

L

M

M

R

K

L

L

R

E

L

I

A

E

Q

T

P

P

S

T

Q

H

L

L

I

I

D

D

V

V

T

N

R

G

I

L

P

G

A

L

L

D

K

K

S

I

I

E

T

V

V

T

D

D

A

A

K

G

T

G

V

L

T

R

I

I

G

G

A

A

A

L

V

V

C

R

H

N

A

T

D

D

V

L

A

A

D

A

H

H

A

E

E

R

L

V

R

R

V

D

L

Y

P

A

G

V

L

L

A

S

D

R

L

A

A

L

H

A

I
x
G

G
x
A

D

S

R

G

Q

Q

V
x
L

R

R

A

N

L

Q

G
x
A

T
|
T

I

T

G
x
A

G
|
G

S
x
N

L

L

A
x
L

N
x
Q

-

R

-

T

-
x
R

-
x
C

-

P

-

Y

-
x
F

-

Y

-

D

-

T

N

N

D

Q

P

P

A
x
C

-
x
N

-
x
K

-

R

-

L

-

P

-

G

-

S

-

G

-
x
C

-
x
A

-

L

-

E

-

G

-

F

-

S

-

R

-

Q

-
x
H

-

A

-

V

-

G

-

V

-

S

-

E

A

A

C
|
C

-
x
I

-
x
A

-

T

Y

H

P
|
P

-
x
S

-
x
D

-

M

A

A

A

V

A

A

M

M

G

R

-

L

L

L

D

D

A

A

T

V

I

V

V

E

T

T

-

I

-

T

-

P

-

E

-

G

D

K

R

T

R

R

R

S

I

I

A

T

S

L

S

A

D

D

F

F

F

Y

-

H

-

P

-

G

-

K

V

T

G

P

M

H

Y

I

E

E

T

T

A

A

L

L

A

L

P

P

D

G

E

E

L
|
L

I
|
I

V

V

A

A

V

V

E

T

S

L

P

P

V

P

P

P

-

L

-

G

E

G

R

K

A

H

A

I

Y

Y

E

R

K
|
K

F

V

R

R

N

D

P

R

A

A

S

S

H
x
Y

-
x
A

F
|
F

A

A

L

L

V

V

G

S

V

V

-

A

F

I

V

I

A

Q

K

P

F

D

A

G

S

S

G

G

V

-

R

R

V

V

A

A

V

L

T

G

G

G

A

V

A

A

S

H

S

K

V

P

F

W

R

R

V

I

P

E

E

A

L

A

E

D

S

A

A

Q

L

L

S

S

binding flavin-adenine dinucleotide: K26 (= K26), F27 (= F27), I28 (≠ L28), A29 (= A29), G30 (= G30), G31 (= G31), T32 (≠ Q32), N33 (≠ S33), L34 (= L34), G98 (≠ I98), A99 (≠ G99), L103 (≠ V103), A107 (≠ G107), T108 (= T108), A110 (≠ G110), G111 (= G111), N112 (≠ S112), L114 (≠ A114), Q115 (≠ N115), R118 (vs. gap), P162 (= P123), S163 (vs. gap), D164 (vs. gap), L212 (= L159), I213 (= I160), K230 (= K175), Y237 (≠ H182), A238 (vs. gap), F239 (= F183)
binding iron/sulfur cluster: C119 (vs. gap), F122 (vs. gap), C129 (≠ A119), N130 (vs. gap), K131 (vs. gap), C138 (vs. gap), A139 (vs. gap), A140 (vs. gap), H148 (vs. gap), C157 (= C121), I158 (vs. gap), A159 (vs. gap)

P77324 Aldehyde oxidoreductase FAD-binding subunit PaoB; EC 1.2.99.6 from Escherichia coli (strain K12) (see paper)
32% identity, 83% coverage: 1:219/265 of query aligns to 1:276/318 of P77324

query
sites
P77324

M

M

Y

K

S

A

F

F

E

T

Y

Y

Q

E

R

R

A

V

T

N

D

T

P

P

K

A

A

E

A

A

A

L

S

S

L

A

T

Q

A

R

D

V

S

P

D

G

A

A

K
|
K

F
|
F

L
x
I

A
|
A

G
|
G

Q
x
T

S
x
N

L
|
L

L

L

P

D

T

L

M

M

R

K

L

L

R

E

L

I

A

E

Q

T

P

P

S

T

Q

H

L

L

I

I

D

D

V

V

T

N

R

G

I

L

P

G

A

L

L

D

K

K

S

I

I

E

T

V

V

T

D

D

A

A

K

G

T

G

V

L

T

R

I

I

G

G

A

A

A

L

V

V

C

R

H

N

A

T

D

D

V

L

A

A

D

A

H

H

A

E

E

R

L

V

R

R

V

D

L

Y

P

A

G

V

L

L

A

S

D

R

L

A

A

L

H

A

I

G

G

A

D

S

R

G

Q

Q

V

L

R

R

A

N

L

Q

G

A

T
|
T

I

T

G

A

G

G

S

N

L

L

A

L

N

Q

-

R

-

T

-

R

-
x
C

-

P

-

Y

-

F

-

Y

-

D

-

T

N

N

D

Q

P

P

A
x
C

-

N

-

K

-

R

-

L

-

P

-

G

-

S

-

G

-
x
C

-

A

-

L

-

E

-

G

-

F

-

S

-

R

-

Q

-

H

-

A

-

V

-

G

-

V

-

S

-

E

A

A

C
|
C

-

I

-

A

-

T

Y

H

P

P

-

S

-
x
D

-

M

A

A

A

V

A

A

M

M

G

R

-

L

L

L

D

D

A

A

T

V

I

V

V

E

T

T

-

I

-

T

-

P

-

E

-

G

D

K

R

T

R

R

R

S

I

I

A

T

S

L

S

A

D

D

F

F

F

Y

-

H

-

P

-

G

-

K

V

T

G

P

M

H

Y

I

E

E

T

T

A

A

L

L

A

L

P

P

D

G

E

E

L

L

I
|
I

V

V

A

A

V

V

E

T

S

L

P

P

V

P

P

P

-

L

-

G

E

G

R

K

A

H

A

I

Y

Y

E

R

K
|
K

F

V

R

R

N

D

P

R

A

A

S

S

H

Y

-

A

F

F

A

A

L

L

V

V

G

S

V

V

-

A

F

I

V

I

A

Q

K

P

F

D

A

G

S

S

G

G

V

-

R

R

V

V

A

A

V

L

T

G

G

G

A

V

A

A

S

H

S

K

V

P

F

W

R

R

V

I

P

E

E

A

L

A

E

D

S

A

A

Q

L

L

S

S

26:34 (vs. 26:34, 67% identical) binding
T108 (= T108) binding
C119 (vs. gap) binding
C129 (≠ A119) binding
C138 (vs. gap) binding
C157 (= C121) binding
D164 (vs. gap) binding
I213 (= I160) binding
K230 (= K175) binding

5y6qB Crystal structure of an aldehyde oxidase from methylobacillus sp. Ky4400 (see paper)
26% identity, 99% coverage: 1:263/265 of query aligns to 1:326/330 of 5y6qB

query
sites
5y6qB

M

M

Y

N

S

P

F

F

E

H

Y

W

Q

K

R

V

A

A

T

Q

D

S

P

A

K

S

A

E

A

A

S

-

L

L

T

K

A

Q

D

Q

S

S

D

G

A

S

K
x
Q

F
x
I

L
|
L

A
|
A

G
|
G

Q
x
T

S
x
T

L
x
Q

L

L

P

D

T

L

M

M

R

K

L

C

R

G

L

V

A

F

Q

N

P

P

S

P

Q

Q

L

L

I

I

D

G

V
x
I

T

N

R

G

I

L

P

S

A

E

L

L

K

R

S

S

I

L

T

S

V

F

D

D

A

N

K

D

T

K

V

I

T

S

I

A

G

G

A

A

A

L

V

I

C

T

H
x
M

A

S

D

Q

V

L

A

A

D

D

H

H

A

P

E

D

L

C

R

Q

V

H

L

A

P

P

G

A

L

I

A

Y

D

G

L

S

A

L

A

W

H

Q

I
x
A

G
x
A

D

S

R

P

Q

Q

V
x
I

R

R

A

N

L

M

G
x
A

T
|
T

I

L

G

G

G
|
G

S
x
N

L

L

A
x
R

N
x
Q

-
x
R

-

T

-

R

-
x
C

-

A

-

Y

-
x
Y

N

R

D

D

P

P

A

A

-

T

-

F

-

S

A

A

C
|
C

-
x
N

-
x
K

-

R

-

N

-

P

-

G

-

S

-

G

-
x
C

-
x
A

-

R

-

Q

-

G

-

V

-

N

-

S

-

N

-
x
H

-

A

-

I

-

L

-

G

-

A

-

S

-

D

-
x
S

-
x
C

-
x
I

-
x
A

-

V

Y

Y

P

P

A
x
G

-
x
D

-

L

-

A

-

V

A

A

A

L

M

V

G

A

L

F

D

D

A

A

T

V

I

V

V

I

T

V

-

Q

-

N

-

P

-

Q

-

G

D

E

R

T

R

R

I

I

A

A

S

I

S

D

D

D

F

F

V

L

G

L

M

P

Y

G

E

E

T

T

A

P

-

D

-

Q

-

E

-

H

-

D

L

L

A

R

P

D

D

D

E

E

L
x
I

I
|
I

V

V

A

A

V

I

E

E

S

V

P

P

V

Q

P

T

-

A

-

S

-

L

E

Q

R

R

A

S

A

H

Y

Y

E

L

K
|
K

F

V

R

R

N

D

P

R

A

S

S

S

H
x
Y

F
x
E

A
x
F

L

A

V

A

G

A

V

S

F

A

V

A

A

A

K

G

F

F

A

T

-

L

-

E

-

N

-

G

-

V

-

M

S

R

G

Q

V

V

R

H

V

I

A

A

V

L

T

G

G

G

A

V

A

A

S

T

S

K

V

P

F

W

R

R

V

A

P

T

E

R

L

V

E

E

S

Q

A

A

L

L

S

E

A

G

N

Q

F

-

T

-

P

P

E

F

A

N

A

E

R

E

G

T

V

V

T

F

V

Q

S

A

A

A

A

E

D

L

L

I

N

N

T

Q

D

E

M

T

H

Q

A

A

S

L

A

E

D

H

-

N

-

A

Y

Y

R

K

A

V

H

K

L

L

I

A

P

P

V

R

L

V

A

I

A

A

R

R

A

A

V

L

T

M

K

A

A

A

binding flavin-adenine dinucleotide: Q25 (≠ K26), I26 (≠ F27), L27 (= L28), A28 (= A29), G29 (= G30), G30 (= G31), T31 (≠ Q32), T32 (≠ S33), Q33 (≠ L34), I50 (≠ V51), M74 (≠ H75), A97 (≠ I98), A98 (≠ G99), I102 (≠ V103), A106 (≠ G107), T107 (= T108), G110 (= G111), N111 (≠ S112), R113 (≠ A114), Q114 (≠ N115), R115 (vs. gap), G164 (≠ A124), D165 (vs. gap), I213 (≠ L159), I214 (= I160), K232 (= K175), Y239 (≠ H182), E240 (≠ F183), F241 (≠ A184)
binding iron/sulfur cluster: C118 (vs. gap), Y121 (vs. gap), C130 (= C121), N131 (vs. gap), K132 (vs. gap), C139 (vs. gap), A140 (vs. gap), A141 (vs. gap), H149 (vs. gap), S157 (vs. gap), C158 (vs. gap), I159 (vs. gap), A160 (vs. gap)

3hrdG Crystal structure of nicotinate dehydrogenase (see paper)
32% identity, 52% coverage: 27:163/265 of query aligns to 29:172/292 of 3hrdG

query
sites
3hrdG

F
x
I

L
x
I

A
|
A

G
|
G

Q
x
T

S
x
D

L
|
L

L

V

P

I

T

E

M

I

R

N

L

D

R

R

L

W

A

E

Q

K

P

P

S

D

Q

V

L

V

I

I

D

D

V
x
I

T

K

R

K

I

L

P

K

A

E

L

L

K

E

S

Y

I

I

T

R

V

V

D

E

A

E

K

N

T

T

V

I

T

H

I

I

G

G

A

A

A

L

V

S

C

T

H
x
F

A

T

D

Q

V

I

A

E

D

N

H

H

A

P

E

F

L

I

R

R

R

S

V

H

L

V

P

R

G

A

L

L

A

Y

D

K

L

A

A

A

A

S

H

Q

I
x
V

G
|
G

D

S

R

P

Q

Q

V
x
I

R

R

A

N

L

L

G
|
G

T
|
T

I

I

G
|
G

S
x
N

L

L

A
x
S

N
x
T

N

S

D

S

P

V

A

A

A
x
G

C
x
D

Y

G

P

V

A

S

A

A

A

M

M

T

G

T

L

L

D

D

A

A

T

T

I

V

V

V

T

L

D

E

R

S

-

V

-

R

-

G

-

T

R

R

R

Q

I

M

A

K

S

L

S

T

D

D

F

F

V

D

G

G

-

E

-

G

-

F

M

K

Y

R

E
x
R

T

N

A

A

L
|
L

A

E

P

A

D

D

E

E

L
x
I

I
x
M

V

T

A

E

V

V

binding flavin-adenine dinucleotide: I29 (≠ F27), I30 (≠ L28), A31 (= A29), G32 (= G30), G33 (= G31), T34 (≠ Q32), D35 (≠ S33), L36 (= L34), I53 (≠ V51), F77 (≠ H75), V100 (≠ I98), G101 (= G99), I105 (≠ V103), G109 (= G107), T110 (= T108), G112 (= G110), G113 (= G111), N114 (≠ S112), S116 (≠ A114), T117 (≠ N115), G122 (≠ A120), D123 (≠ C121), R160 (≠ E151), L163 (= L154), I168 (≠ L159), M169 (≠ I160)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 187

Q0QLF4 Nicotinate dehydrogenase FAD-subunit; NDH; Nicotinic acid hydroxylase FAD-subunit; NAH; EC 1.17.1.5 from Eubacterium barkeri (Clostridium barkeri) (see paper)
32% identity, 52% coverage: 27:163/265 of query aligns to 29:172/296 of Q0QLF4

query
sites
Q0QLF4

F
x
I

L
x
I

A
|
A

G
|
G

Q
x
T

S
x
D

L
|
L

L

V

P

I

T

E

M

I

R

N

L

D

R

R

L

W

A

E

Q

K

P

P

S

D

Q

V

L

V

I

I

D

D

V

I

T

K

R

K

I

L

P

K

A

E

L

L

K

E

S

Y

I

I

T

R

V

V

D

E

A

E

K

N

T

T

V

I

T

H

I

I

G

G

A

A

A

L

V

S

C

T

H

F

A

T

D

Q

V

I

A

E

D

N

H

H

A

P

E

F

L

I

R

R

R

S

V

H

L

V

P

R

G

A

L

L

A

Y

D

K

L

A

A

A

A

S

H

Q

I

V

G
|
G

D

S

R

P

Q

Q

V

I

R

R

A

N

L

L

G

G

T
|
T

I
|
I

G
|
G

S
x
N

L

L

A

S

N

T

N

S

D

S

P

V

A

A

A

G

C
x
D

Y

G

P

V

A

S

A

A

A

M

M

T

G

T

L

L

D

D

A

A

T

T

I

V

V

V

T

L

D

E

R

S

-

V

-

R

-

G

-

T

R

R

R

Q

I

M

A

K

S

L

S

T

D

D

F

F

V

D

G

G

-

E

-

G

-

F

M

K

Y

R

E
x
R

T

N

A

A

L

L

A

E

P

A

D

D

E

E

L

I

I
x
M

V

T

A

E

V

V

29:36 (vs. 27:34, 50% identical) binding
G101 (= G99) binding
TIGGN 110:114 (≠ TIGGS 108:112) binding
D123 (≠ C121) binding
R160 (≠ E151) binding
M169 (≠ I160) binding

Sites not aligning to the query:

187 binding

1rm6B Structure of 4-hydroxybenzoyl-coa reductase from thauera aromatica (see paper)
29% identity, 75% coverage: 28:227/265 of query aligns to 30:281/323 of 1rm6B

query
sites
1rm6B

L
|
L

A
x
G

G
x
A

G
|
G

Q
x
T

S
x
D

L
|
L

L

L

P

P

T

N

M

L

R

R

L

R

R

G

L

L

A

G

Q

H

P

P

S

A

Q

A

L

L

I

V

D

D

V
x
L

T

T

R

G

I

I

P

D

A

G

L

L

K

A

S

T

I

I

T

S

V

T

D

L

A

A

K

D

-

G

T

S

V

L

T

R

I

I

G

G

A

A

A

G

V

A

C

T

H

L

A

E

D

A

V

I

A

A

D

E

H

H

A

D

E

A

L

I

R

R

R

T

V

T

L

W

P

P

G

A

L

L

A

A

D

Q

L

A

A

A

A

E

H

S

I
x
V

G
x
A

D

G

R

P

Q

T

V
x
H

R

R

A

A

L

A

G
x
A

T
|
T

I

L

G
|
G

S
x
N

L

L

A
x
C

N
x
Q

N

D

D

T

P

R

A
x
C

A

T

-
x
F

-

Y

-

N

-

Q

-

S

-

E

-

W

-

R

-

S

-

G

-

N

-

G

-

Y

-
x
C

-
x
L

-
x
K

-

Y

-

K

-

G

-

D

-

K

-
x
C

-
x
H

-
x
V

-

I

-

V

-

K

-

S

-

D

-

R

C
|
C

Y
|
Y

P
x
A

A

T

-

Y

-
x
H

-
x
G

-
x
D

-

V

-

A

-

P

A

A

A

L

M

M

G

V

L

L

D

D

-

A

-

R

A

A

T

E

I

I

V

V

-

G

-

P

T

A

D

G

R

K

R

R

R

T

I

V

A

P

S

V

S

A

D

Q

F

L

F

F

-

R

-

E

V

S

G

G

M

A

Y

E

E

H

T

L

A

T

L

L

A

E

P

K

D

G

E

E

L
|
L

I
x
L

V

A

A

A

V

I

E

E

S

V

P

P

V

P

P

T

-

G

-

A

E

W

R

S

A

A

Y

Y

E

S

K
|
K

F

V

R

R

-

I

N

R

P

D

A

A

S
x
V

H
x
D

F
|
F

A
x
P

L

L

V

A

G

G

V

V

F

A

V

A

A

A

-

L

-

Q

-

R

-

D

-

G

K

D

F

R

A

I

S

A

G

G

V

L

R

R

V

V

A

A

V

I

T

T

G

G

A

S

A

N

S

S

S

A

V

P

F

L

R

M

V

V

P

P

E

-

L

V

E

D

S

A

A

L

L

L

S

G

A

G

N

N

F

W

T

D

P

D

E

A

A

A

binding flavin-adenine dinucleotide: L30 (= L28), G31 (≠ A29), A32 (≠ G30), G33 (= G31), T34 (≠ Q32), D35 (≠ S33), L36 (= L34), L53 (≠ V51), V101 (≠ I98), A102 (≠ G99), H106 (≠ V103), A110 (≠ G107), T111 (= T108), G113 (= G110), G114 (= G111), N115 (≠ S112), C117 (≠ A114), Q118 (≠ N115), H160 (vs. gap), G161 (vs. gap), D162 (vs. gap), L206 (= L159), L207 (≠ I160), K224 (= K175), V231 (≠ S181), D232 (≠ H182), F233 (= F183), P234 (≠ A184)
binding iron/sulfur cluster: C122 (≠ A119), F124 (vs. gap), C138 (vs. gap), L139 (vs. gap), K140 (vs. gap), C146 (vs. gap), H147 (vs. gap), V148 (vs. gap), C155 (= C121), Y156 (= Y122), A157 (≠ P123)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 29

O33820 4-hydroxybenzoyl-CoA reductase subunit beta; 4-HBCR subunit beta; EC 1.1.7.1 from Thauera aromatica (see paper)
29% identity, 75% coverage: 28:227/265 of query aligns to 30:281/324 of O33820

query
sites
O33820

L
|
L

A
x
G

G
x
A

G
|
G

Q
x
T

S
x
D

L
|
L

L

L

P

P

T

N

M

L

R

R

L

R

R

G

L

L

A

G

Q

H

P

P

S

A

Q

A

L

L

I

V

D

D

V

L

T

T

R

G

I

I

P

D

A

G

L

L

K

A

S

T

I

I

T

S

V

T

D

L

A

A

K

D

-

G

T

S

V

L

T

R

I

I

G

G

A

A

A

G

V

A

C

T

H

L

A

E

D

A

V

I

A

A

D

E

H

H

A

D

E

A

L

I

R

R

R

T

V

T

L

W

P

P

G

A

L

L

A

A

D

Q

L

A

A

A

A

E

H

S

I

V

G

A

D

G

R

P

Q

T

V

H

R

R

A

A

L

A

G

A

T
|
T

I

L

G

G

S
x
N

L

L

A

C

N
x
Q

N

D

D

T

P

R

A

C

A

T

-

F

-

Y

-

N

-

Q

-

S

-

E

-

W

-

R

-

S

-

G

-

N

-

G

-

Y

-

C

-

L

-

K

-

Y

-

K

-

G

-

D

-

K

-

C

-

H

-

V

-

I

-

V

-

K

-

S

-

D

-

R

C

C

Y

Y

P

A

A

T

-

Y

-

H

-

G

-
x
D

-

V

-

A

-

P

A

A

A

L

M

M

G

V

L

L

D

D

-

A

-

R

A

A

T

E

I

I

V

V

-

G

-

P

T

A

D

G

R

K

R

R

R

T

I

V

A

P

S

V

S

A

D

Q

F

L

F

F

-

R

-

E

V

S

G

G

M

A

Y

E

E

H

T

L

A

T

L

L

A

E

P

K

D

G

E

E

L

L

I

L

V

A

A

A

V

I

E

E

S

V

P

P

V

P

P

T

-

G

-

A

E

W

R

S

A

A

Y

Y

E

S

K
|
K

F

V

R

R

-

I

N

R

P

D

A

A

S

V

H

D

F

F

A

P

L

L

V

A

G

G

V

V

F

A

V

A

A

A

-

L

-

Q

-

R

-

D

-

G

K

D

F

R

A

I

S

A

G

G

V

L

R

R

V

V

A

A

V

I

T

T

G

G

A

S

A

N

S

S

S

A

V

P

F

L

R

M

V

V

P

P

E

-

L

V

E

D

S

A

A

L

L

L

S

G

A

G

N

N

F

W

T

D

P

D

E

A

A

A

T111 (= T108) binding
N115 (≠ S112) binding
Q118 (≠ N115) binding
D162 (vs. gap) binding
K224 (= K175) binding

Sites not aligning to the query:

29:36 binding

2w54A Crystal structure of xanthine dehydrogenase from rhodobacter capsulatus in complex with bound inhibitor pterin-6-aldehyde (see paper)
29% identity, 67% coverage: 89:265/265 of query aligns to 249:440/450 of 2w54A

query
sites
2w54A

P

P

G

A

L

L

A

A

D

G

L

L

A

L

A

R

H

R

I
x
F

G
x
A

D

S

R

E

Q

Q

V
|
V

R

R

A

Q

L

V

G
x
A

T
|
T

I

I

G

G

G
|
G

S
x
N

L

I

A
|
A

N
|
N

N

G

D

S

P

P

A

I

A
x
G

C
x
D

Y

G

P

P

A

P

A

A

A

L

M

I

G

A

L

M

D

G

A

A

T

S

I

L

V

T

-

L

-

R

-

G

T

Q

D

E

R

R

I

M

A

P

S

L

S

E

D

D

F

F

V

L

G

E

M

Y

Y

R

E

K

T

Q

A

D

L
x
R

A

R

P

P

D

G

E

E

L
x
F

I
x
V

V

E

A

S

V

V

E

T

S

L

P

P

-

K

-

S

V

A

P

P

E

G

R

L

A

R

A

C

Y

Y

-
x
K

-

L

-

S

E

K

K

R

F

F

R

D

N
x
Q

P

D

A

I

S

S

H

-

F

-

A

A

L

V

V

C

G

G

V

C

F

L

V

N

A

L

K

T

F

L

A

K

S

G

G

S

-

K

-

I

-

E

-

T

V

A

R

R

V

I

A

A

V

F

T

G

G

G

A

M

A

A

S

G

S

-

V

-

F

-

R

-

V

V

P

P

E

K

L

R

E

A

S

A

A

A

L

F

S

E

A

A

N

A

F

L

T

I

P

G

E

Q

A

D

A

F

R

R

G

E

V

D

T

T

V

I

S

A

A

A

-

L

-

P

-

L

-

A

-

Q

D

D

L

F

N

T

-

P

-

L

T

S

D

D

M

M

H

R

A

A

S

S

A

A

D

A

Y

Y

R

R

A

M

H

N

L

A

I

A

P

Q

V

A

L

M

A

A

A

L

R

R

A

Y

V

V

T

R

K

E

A

L

N

S

G

G

binding flavin-adenine dinucleotide: F258 (≠ I98), A259 (≠ G99), V263 (= V103), A267 (≠ G107), T268 (= T108), G271 (= G111), N272 (≠ S112), A274 (= A114), N275 (= N115), G280 (≠ A120), D281 (≠ C121), R318 (≠ L154), F323 (≠ L159), V324 (≠ I160), K340 (vs. gap), Q347 (≠ N178)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 41, 42, 43, 189, 191, 192, 193, 194, 195, 196, 199, 213
binding fe2/s2 (inorganic) cluster: 37, 38, 39, 40, 42, 44, 45, 47, 63, 102, 103, 104, 106, 134, 135, 136
binding {[(5aR,8R,9aR)-2-amino-4-oxo-6,7-di(sulfanyl-kappaS)-3,5,5a,8,9a,10-hexahydro-4H-pyrano[3,2-g]pteridin-8-yl]methyl dihydrogenato(2-) phosphate}(hydroxy)oxo(thioxo)molybdenum: 102, 136

1jroA Crystal structure of xanthine dehydrogenase from rhodobacter capsulatus (see paper)
29% identity, 67% coverage: 89:265/265 of query aligns to 249:440/450 of 1jroA

query
sites
1jroA

P

P

G

A

L

L

A

A

D

G

L

L

A

L

A

R

H

R

I
x
F

G
x
A

D

S

R

E

Q

Q

V
|
V

R

R

A

Q

L

V

G
x
A

T
|
T

I

I

G

G

G
|
G

S
x
N

L

I

A
|
A

N
|
N

N

G

D

S

P

P

A

I

A
x
G

C
x
D

Y

G

P

P

A

P

A

A

A

L

M

I

G

A

L

M

D

G

A

A

T

S

I

L

V

T

-

L

-

R

-

G

T

Q

D

E

R

R

I

M

A

P

S

L

S

E

D

D

F

F

V

L

G

E

M

Y

Y

R

E

K

T

Q

A

D

L
x
R

A

R

P

P

D

G

E

E

L
x
F

I
x
V

V

E

A

S

V

V

E

T

S

L

P

P

-

K

-

S

V

A

P

P

E

G

R

L

A

R

A

C

Y

Y

-
x
K

-

L

-

S

E

K

K

R

F

F

R

D

N
x
Q

P

D

A

I

S

S

H

-

F

-

A

A

L

V

V

C

G

G

V

C

F

L

V

N

A

L

K

T

F

L

A

K

S

G

G

S

-

K

-

I

-

E

-

T

V

A

R

R

V

I

A

A

V

F

T

G

G

G

A

M

A

A

S

G

S

-

V

-

F

-

R

-

V

V

P

P

E

K

L

R

E

A

S

A

A

A

L

F

S

E

A

A

N

A

F

L

T

I

P

G

E

Q

A

D

A

F

R

R

G

E

V

D

T

T

V

I

S

A

A

A

-

L

-

P

-

L

-

A

-

Q

D

D

L

F

N

T

-

P

-

L

T

S

D

D

M

M

H

R

A

A

S

S

A

A

D

A

Y

Y

R

R

A

M

H

N

L

A

I

A

P

Q

V

A

L

M

A

A

A

L

R

R

A

Y

V

V

T

R

K

E

A

L

N

S

G

G

binding flavin-adenine dinucleotide: F258 (≠ I98), A259 (≠ G99), V263 (= V103), A267 (≠ G107), T268 (= T108), G271 (= G111), N272 (≠ S112), A274 (= A114), N275 (= N115), G280 (≠ A120), D281 (≠ C121), R318 (≠ L154), F323 (≠ L159), V324 (≠ I160), K340 (vs. gap), Q347 (≠ N178)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 41, 42, 43, 189, 190, 191, 192, 193, 194, 195, 196, 199, 213
binding fe2/s2 (inorganic) cluster: 37, 38, 39, 40, 42, 43, 44, 45, 47, 63, 102, 103, 104, 106, 134, 136

2ckjA Human milk xanthine oxidoreductase
24% identity, 74% coverage: 4:199/265 of query aligns to 200:407/1264 of 2ckjA

query
sites
2ckjA

F

F

E

E

Y

G

Q

E

R

R

A

V

T

T

D

W

P

I

K

Q

A

A

A

S

A

T

A

L

S

K

L

E

T

L

A

L

D

D

-

L

-

K

-

A

-

Q

-

H

S

P

D

D

A

A

K

K

F
x
L

L
x
V

A
x
V

G
|
G

G
x
N

Q
x
T

S
x
E

L
x
I

L

G

P

I

T

E

M

M

R

K

L

F

R

K

L

N

A

M

Q

L

P

F

S

P

Q

M

L

I

I

V

D

C

V

P

T

A

R

W

I

I

P

P

A

E

L

L

K

N

S

S

I

V

T

E

V

H

D

G

A

P

K

D

T

G

V

I

T

S

I

F

G

G

A

A

V

-

C

C

H

P

A

L

D

S

V

I

A

V

D

E

H

K

-

T

-

L

-

V

-

D

-

A

-

V

-

A

-

K

-

L

-

P

A

A

E

Q

L

K

R

T

R

E

V

V

L

F

P

R

G

G

L

V

A

L

D

E

L

Q

A

L

A

R

H

W

I
x
F

G
x
A

D

G

R

K

Q

Q

V
|
V

R

K

A

S

L

V

G
x
A

T
x
S

I

V

G

G

G
|
G

S
x
N

L

I

A
x
I

N
x
T

N

A

D

S

P

P

A

I

A
x
S

C
x
D

Y

L

P

N

A

P

A

V

A

F

M

M

-

A

-

S

G

G

L

A

D

K

A

L

T

T

I

L

V

V

T

S

-

R

D

G

R

T

R

R

I

T

A

V

S

Q

S

M

D

D

-

H

-

T

F

F

V

P

G

G

M

Y

Y

R

E

K

T

T

A

L

L

L

A

S

P

P

D

E

E

E

L
x
I

I
x
L

V

L

A

S

V

I

E

E

S

I

P

P

V

Y

P

S

E

R

R

E

A

G

A

E

Y

Y

E

F

K

S

F

A

R

F

N
x
K

P

Q

A

A

S

S

H

D

F

-

A

-

L

-

V

-

G

-

V

-

F

-

V

I

A

A

K

K

F

V

A

T

S

S

G

G

V

M

R

R

V

V

binding flavin-adenine dinucleotide: L228 (≠ F27), V229 (≠ L28), V230 (≠ A29), G231 (= G30), N232 (≠ G31), T233 (≠ Q32), E234 (≠ S33), I235 (≠ L34), F308 (≠ I98), A309 (≠ G99), V313 (= V103), A317 (≠ G107), S318 (≠ T108), G321 (= G111), N322 (≠ S112), I324 (≠ A114), T325 (≠ N115), S330 (≠ A120), D331 (≠ C121), I374 (≠ L159), L375 (≠ I160), K393 (≠ N178)

Sites not aligning to the query:

active site: 711, 746, 824, 828, 856, 1204, 1205
binding flavin-adenine dinucleotide: 44
binding fe2/s2 (inorganic) cluster: 39, 40, 41, 42, 44, 46, 47, 49, 71, 110, 111, 112, 114, 146, 147, 148

Q8GUQ8 Xanthine dehydrogenase 1; AtXDH1; EC 1.17.1.4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
25% identity, 57% coverage: 24:175/265 of query aligns to 282:447/1361 of Q8GUQ8

query
sites
Q8GUQ8

D

D

A

A

K

K

F

L

L

L

A

V

G

G

G

N

Q

T

S

E

L

V

L

G

P

I

T

E

M

M

R

R

L

L

R

K

L

R

A

L

Q

Q

P

Y

S

Q

Q

V

L

L

I

I

D

S

V

V

T

A

R

Q

I

V

P

P

A

E

L

L

K

N

S

A

I

L

T

N

V

V

D

N

A

D

K

N

T

G

V

I

T

E

I

V

G

G

A

S

A

A

V

L

C

R

H

L

A

S

D

E

V

L

A

-

D

-

H

-

A

-

E

-

L

L

R

R

R

L

V

F

L

R

P

K

G

I

L

V

A

K

D

E

L

R

A

P

A

A

H

H

-

E

-

T

-

S

-

A

-

C

-

K

-

A

-

F

-

I

-

E

-

Q

-

L

-

K

-
x
W

I

F

G

A

D

G

R

T

Q

Q

V

I

R

R

A

N

L

V

G

A

T

C

I

I

G

G

S

N

L

I

A

C

N

T

N

A

D

S

P

P

A

I

A

S

C

D

Y

L

P

N

A

P

A

L

A

W

M

M

G

A

L

S

D

R

A

A

T

E

I

F

-

R

V

I

T

T

-

N

-

C

-

N

-

G

D

D

R

V

R

R

R

S

I

I

A

P

S

A

S

K

D

D

F

F

V

L

G

G

M
x
Y

Y

R

E

K

T

V

A

D

L

M

A

G

P

S

D

N

E

E

L

I

I

L

V

L

A

S

V

V

E

F

S

L

P

P

V

W

P

T

E

R

R

P

A

L

A

E

Y

Y

E

V

K

K

W364 (vs. gap) mutation to A: Decreases activity 8-fold.
Y421 (≠ M149) mutation to A: Decreases activity 4-fold.

Sites not aligning to the query:

831 E→A: Loss of activity.
909 R→A: Decreases activity 12-fold.
1297 E→A: Decreases activity 40-fold.

P47989 Xanthine dehydrogenase/oxidase; EC 1.17.1.4; EC 1.17.3.2 from Homo sapiens (Human) (see 4 papers)
24% identity, 74% coverage: 4:199/265 of query aligns to 229:440/1333 of P47989

query
sites
P47989

F

F

E

E

Y

G

Q

E