SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS03430 FitnessBrowser__BFirm:BPHYT_RS03430 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see paper)
29% identity, 40% coverage: 32:201/425 of query aligns to 56:207/444 of Q8NLB7

query
sites
Q8NLB7

F

Y

D
|
D

L

L

V

I

V

V

Y

Y

G

G

F

T

F

V

A

Q

V

S

I

A

I

L

A

A

K

K

L

E

F

W

F

-

P

-

A

-

G

-

N

N

D

L

T

S

V

S

S

A

L

T

L

L

L

G

T

T

L

I

G

G

T

S

F

-

G

-

V

T

S

A

F

F

F

F

M

G

R

M

P

A

L

I

G

G

A

A

I

V

V

F

I

I

G

G

A

R

Y

L

A

S

D

D

R
|
R

A

V

G

G

R

R

K

K

A

A

A

A

L

V

T

I

L

G

S

S

I

V

L

L

L

I

M

L

M

S

G

V

G

F

T

T

L

M

I

L

I

C

A

A

I

F

L

A

P

P

T

N

Y

-

Q

-

S

-

I

-

G

-

L

-

A

-

A

-

P

P

V

W

I

V

L

F

V

G

L

A

A

F

R

R

L

F

M

I

Q

A

G

G

F

L

S

G

A

L

G

G

G

G

E

L

F

V

G

P

S

S

A

V

T

N

A

A

F

M

L

T

A

S

E

D

H

L

V

V

P

P

G

-

R

-

R

R

G

K

F

T

F

M

A

S

S

A

W

W

-

A

Q

T

V

V

A

M

S

M

Q

S

G

G

L

V

T

P

T

-

L

-

L

I

A

G

A

G

G

S

F

I

G

A

V

A

L

V

L

L

T

A

G

L

K

V

L

V

S

V

P

P

E

S

Q

S

M

-

A

E

S

E

W

W

G

G

W

W

R

R

V

F

P

M

F

F

F

L

F

I

G

A

L

L

L

I

D57 (= D33) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
R103 (= R85) mutation to A: Loss of transport activity.

Sites not aligning to the query:

54 D→A: Loss of transport activity.; D→E: Retains 50% of its transport activity.
309 W→V: Loss of transport activity.
312 D→A: Loss of transport activity.
313 R→A: Loss of transport activity.
317 mutation I->H,Y: Loss of transport activity.
386 R→A: Loss of transport activity.

Query Sequence

>BPHYT_RS03430 FitnessBrowser__BFirm:BPHYT_RS03430
MNATTAASLAGSRQNSWRAVIAASIGNALEWFDLVVYGFFAVIIAKLFFPAGNDTVSLLL
TLGTFGVSFFMRPLGAIVIGAYADRAGRKAALTLSILLMMGGTLIIAILPTYQSIGLAAP
VILVLARLMQGFSAGGEFGSATAFLAEHVPGRRGFFASWQVASQGLTTLLAAGFGVLLTG
KLSPEQMASWGWRVPFFFGLLIGPVAWYIRTKLDETPEFLAAETTETPLRDTFTSQKLRL
LIAIGVVVLGTVSTYLVLFMPTFGVKQLGLAPSVAFSAIALTGLIQMVFSPLVGHLSDRH
GRTTIMLSSALLLLVLIYPAFVYLVAHPTFGTLIAMQIVFGFLMTGYFAALPGLLSEMFP
VQTRTTGMSLAYNIAVTIFGGFGPFIIAWLISVTGSKAAPSYYLIFAAIVSLVALTAARR
KLGFR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory