SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS03850 BPHYT_RS03850 aspartate aminotransferase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q53IZ1 Bifunctional aspartate aminotransferase and L-aspartate beta-decarboxylase; Aspartate 4-decarboxylase; Asd; AsdP; EC 2.6.1.1; EC 4.1.1.12 from Pseudomonas sp. (see paper)
60% identity, 95% coverage: 18:542/554 of query aligns to 8:527/531 of Q53IZ1

query
sites
Q53IZ1

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SYM 67:69 (≠ AYM 82:84) mutation to EEE: Loss of activity. Dissociation into dimers.
K314 (= K328) modified: N6-(pyridoxal phosphate)lysine

2zy2A Dodecameric l-aspartate beta-decarboxylase (see paper)
60% identity, 94% coverage: 22:542/554 of query aligns to 1:516/521 of 2zy2A

query
sites
2zy2A

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binding pyridoxal-5'-phosphate: R26 (= R52), G161 (= G187), G162 (= G188), T163 (= T189), F192 (= F218), Y195 (= Y221), N244 (= N270), D274 (= D299), V276 (= V301), Y277 (= Y302), S300 (= S325), S302 (= S327), K303 (= K328), R311 (= R336)

2zy4F Dodecameric l-aspartate beta-decarboxylase (see paper)
57% identity, 95% coverage: 18:541/554 of query aligns to 3:522/535 of 2zy4F

query
sites
2zy4F

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C

V
|
V

N

N

P

P

S

S

N
|
N

P

P

S

S

V

V

K

K

M

M

D

D

D

Q

E

R

S

S

L

L

R

E

Y

R

I

V

A

R

D

N

I

I

V

V

K

A

E

E

-

H

R

R

P

P

D

D

L

L

I

M

L

I

L

L

T

T

D
|
D

D

D

V
|
V

Y
|
Y

G

G

T

T

F

F

A

A

D

D

F

F

V

Q

S

S

L

L

F

F

A

A

L

I

A

C

P

P

K

E

N

N

T

T

I

L

L

L

V

V

Y

Y

S
|
S

Y

F

S
|
S

K
|
K

Y

Y

F

F

G

G

A

A

T

T

G

G

W

W

R
|
R

L

L

G

G

T

V

I

V

A

A

T

A

H

H

R

Q

D

Q

N

N

V

V

L

F

D

D

K

L

L

A

I

L

A

D

D

K

L

L

P

Q

K

E

D

S

A

E

K

K

K

V

Q

A

L

L

H

D

E

H

R

R

Y

Y

E

R

S

S

I

L

T

L

T

P

E

D

P

V

D

R

K

S

L

L

K

K

F

F

I

I

D

D

R

R

L

L

V

V

A

A

D

D

S

S

R

R

T

A

V

V

A

A

L

L

N

N

H

H

T

T

A

A

G

G

L

L

S

S

T

T

P

P

Q

Q

V

V

Q

Q

M

M

V

A

L

L

F

F

S

S

L

L

F

F

S

A

L

L

M

M

D

D

T

E

P

A

D

D

A

E

Y

Y

K

K

N

H

A

T

L

L

K

K

R

Q

L

L

I

I

R

R

K

R

R

R

K

E

Q

T

A

T

L

L

Y

Y

E

R

E

E

V

L

G

G

I

M

S

P

F

P

K

L

D

R

D

D

D

E

P

-

N

N

Q

A

V

V

D

D

Y

Y

T

T

I

L

L

I

D

D

V

L

E

Q

F

D

L

V

G

T

E

A

R

K

M

L

F

Y

G

G

R

E

E

A

F

F

V

S

D

E

W

W

L

A

L

V

K

K

N

Q

T

S

E

S

P

T

S

G

E

D

L

M

L

L

F

F

R

R

L

I

A

A

R

D

E

E

A

T

R

G

V

I

V

V

L

L

P

P

G

G

L

R

G

G

F

F

G

G

T

S

Q

N

H

R

P

P

S

S

G

G

R

R

V

A

S

S

L

L

A

A

N

N

L

L

N

N

E

E

S

Y

D

E

Y

Y

R

A

Q

A

I

I

G

G

R

R

A

A

M

L

R

R

K

K

L

M

I

A

E

D

E

E

L

L

V

Y

E

A

R

E

F

Y

N

S

binding pyridoxal-5'-phosphate: R32 (= R52), G168 (= G187), G169 (= G188), T170 (= T189), F199 (= F218), V247 (= V266), N251 (= N270), D281 (= D299), V283 (= V301), Y284 (= Y302), S307 (= S325), S309 (= S327), K310 (= K328), R318 (= R336)

Q93QX0 Bifunctional aspartate aminotransferase and L-aspartate beta-decarboxylase; Aspartate 4-decarboxylase; ASD; AsdA; EC 2.6.1.1; EC 4.1.1.12 from Comamonas testosteroni (Pseudomonas testosteroni) (see paper)
57% identity, 95% coverage: 18:541/554 of query aligns to 8:527/533 of Q93QX0

query
sites
Q93QX0

M

L

A

A

A

K

L

L

S

S

P

P

F

F

E

E

L

L

K

K

D

D

E

E

L

L

I

I

K

K

A

I

A

A

G

S

A

-

V

-

E

-

R

-

P

D

A

G

N

N

A

R

S

L

M

M

L

L

N

N

A

A

G

G

R

R

G

G

N

N

P

P

N

N

F

F

L

L

A

A

T

T

I

T

P

P

R

R

H

R

G

A

F

F

W

F

Q

R

L

L

G

G

L

L

F

F

A

A

M

A

R

A

E

E

S

S

E

E

R

L

S

S

F

Y

A

S

Y

Y

M

M

P

T

E

T

-

V

G

G

V

V

G

G

F

L

P

A

R

K

R

I

E

D

G

G

L

I

E

E

E

G

R

R

F

F

D

E

L

R

F

Y

L

I

R

A

E

E

N

N

K

R

G

D

V

Q

A

E

G

G

I

V

D

R

F

F

L

L

R

G

G

K

A

S

V

L

S

S

Y

Y

V

V

R

R

D

D

Q

Q

L

L

G

G

Y

L

S

D

A

P

G

A

D

A

F

F

L

L

Y

H

E

E

M

M

C

V

E

D

G

G

I

I

L

L

A

G

S

C

N

N

Y
|
Y

P

P

V

V

P

P

D

P

R

R

M

M

L

L

K

N

L

I

S

S

E

E

L

K

I

I

V

V

G

R

Q

Q

Y

Y

L

I

R

I

K

R

E

E

M

M

I

G

G

A

D

D

H

A

P

I

F

P

V

S

G

E

E

S

F

V

D

N

V

L

F

F

A

A

V

V

E

E

G

G

T

T

A

A

M

M

T

A

Y

Y

I

I

F

F

N

E

T

S

M

L

R

K

E

L

N

N

H

G

L

L

I

L

K

K

A

A

G

G

D

D

T

K

I

V

A

A

L

I

G

G

M

M

P

P

I

V

F

F

T

T

P

P

Y

Y

I

I

E

E

I

I

P

P

R

E

L

L

N

A

D

Q

Y

Y

R

A

L

L

N

E

V

E

V

V

N

A

L

I

E

N

A

A

D

D

V

P

A

S

N

L

G

N

W

W

Q

Q

Y

Y

S

P

K

D

K

S

E

E

L

L

D

D

K

K

L

L

R

K

D

D

P

P

K

A

V

I

K

K

A

I

F

F

L

C

V

V

N

N

P

P

S

S

N

N

P

P

S

S

V

V

K

K

M

M

D

D

D

Q

E

R

S

S

L

L

R

E

Y

R

I

V

A

R

D

N

I

I

V

V

K

A

E

E

-

H

R

R

P

P

D

D

L

L

I

M

L

I

L

L

T

T

D

D

V

V

Y

Y

G

G

T

T

F

F

A

A

D

D

F

F

V

Q

S

S

L

L

F

F

A

A

L

I

A

C

P

P

K

E

N

N

T

T

I

L

L

L

V

V

Y

Y

S

S

Y

F

S

S

K
|
K

Y

Y

F

F

G

G

A

A

T

T

G

G

W

W

R

R

L

L

G

G

T

V

I

V

A

A

T

A

H

H

R

Q

D

Q

N

N

V

V

L

F

D

D

K

L

L

A

I

L

A

D

D

K

L

L

P

Q

K

E

D

S

A

E

K

K

K

V

Q

A

L

L

H

D

E

H

R

R

Y

Y

E

R

S

S

I

L

T

L

T

P

E

D

P

V

D

R

K

S

L

L

K

K

F

F

I

I

D

D

R

R

L

L

V

V

A

A

D

D

S

S

R

R

T

A

V

V

A

A

L

L

N

N

H

H

T

T

A

A

G

G

L

L

S

S

T

T

P

P

Q

Q

V

V

Q

Q

M

M

V

A

L

L

F

F

S

S

L

L

F

F

S

A

L

L

M

M

D

D

T

E

P

A

D

D

A

E

Y

Y

K

K

N

H

A

T

L

L

K

K

R

Q

L

L

I

I

R

R

K

R

R

R

K

E

Q

T

A

T

L

L

Y

Y

E

R

E

E

V

L

G

G

I

M

S

P

F

P

K

L

D

R

D

D

D

E

P

-

N

N

Q

A

V

V

D

D

Y

Y

T

T

I

L

L

I

D

D

V

L

E

Q

F

D

L

V

G

T

E

A

R

K

M

L

F

Y

G

G

R

E

E

A

F

F

V

S

D

E

W

W

L

A

L

V

K

K

N

Q

T

S

E

S

P

T

S

G

E

D

L

M

L

L

F

F

R

R

L

I

A

A

R

D

E

E

A

T

R

G

V

I

V

V

L

L

P

P

G

G

L
x
R

G

G

F

F

G

G

T

S

Q

N

H

R

P

P

S

S

G

G

R

R

V

A

S

S

L

L

A

A

N

N

L

L

N

N

E

E

S

Y

D

E

Y

Y

R

A

Q

A

I

I

G

G

R

R

A

A

M

L

R

R

K

K

L

M

I

A

E

D

E

E

L

L

V

Y

E

A

R

E

F

Y

N

S

Y134 (= Y148) mutation to F: Slightly reduced activity.
K315 (= K328) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Slightly reduced activity.
R487 (≠ L501) mutation to A: Loss of activity.

5wmiA Arabidopsis thaliana prephenate aminotransferase mutant- t84v (see paper)
30% identity, 25% coverage: 204:341/554 of query aligns to 109:249/402 of 5wmiA

query
sites
5wmiA

L

V

I

C

K

S

A

P

G

G

D

D

T

E

I

V

A

I

L

I

G

P

M

A

P

P

I

Y

F
x
W

T

V

P

S

Y

Y

I

T

E

E

I

Q

P

A

R

R

L

L

N

A

D

D

Y

-

R

-

L

A

N

T

V

P

V

V

N

V

L

I

E

P

A

T

D

K

V

I

A

S

N

N

G

N

W

F

Q

L

Y

L

S

D

K

P

K

K

E

D

L

L

D

E

K

S

L

K

R

L

D

T

P

E

K

K

V

S

K

R

A

L

F

L

F

I

L

L

V

C

N

S

P

P

S

S

N
|
N

P

P

P

T

S

G

V

S

K

V

M

Y

D

P

D

K

E

S

S

L

L

L

R

E

Y

E

I

I

A

A

D

R

I

I

V

I

K

A

E

K

R

H

P

P

D

R

L

L

I

L

L

V

L

L

T

S

D

D

D

E

V

I

Y

Y

G

E

-

H

-

I

T

I

F

Y

A

A

D

P

D

A

F

T

V

H

S

T

L

S

F

F

A

A

L

S

A

L

P

P

-

D

-

M

-

Y

K

E

N

R

T

T

I

L

L

T

V

V

Y

N

S

G

Y

F

S

S

K
|
K

Y

A

F

F

G

A

A

M

T

T

G

G

W

W

R

R

L

L

G

G

T

Y

I

L

A

A

binding 2-oxoglutaric acid: W123 (≠ F218), N173 (= N270), K236 (= K328)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 15, 36, 37, 325, 375

5wmhA Arabidopsis thaliana prephenate aminotransferase (see paper)
30% identity, 25% coverage: 204:341/554 of query aligns to 109:249/399 of 5wmhA

query
sites
5wmhA

L

V

I

C

K

S

A

P

G

G

D

D

T

E

I

V

A

I

L

I

G

P

M

A

P

P

I

Y

F
x
W

T

V

P

S

Y

Y

I

T

E

E

I

Q

P

A

R

R

L

L

N

A

D

D

Y

-

R

-

L

A

N

T

V

P

V

V

N

V

L

I

E

P

A

T

D

K

V

I

A

S

N

N

G

N

W

F

Q

L

Y

L

S

D

K

P

K

K

E

D

L

L

D

E

K

S

L

K

R

L

D

T

P

E

K

K

V

S

K

R

A

L

F

L

F

I

L

L

V

C

N

S

P

P

S

S

N
|
N

P

P

P

T

S

G

V

S

K

V

M

Y

D

P

D

K

E

S

S

L

L

L

R

E

Y

E

I

I

A

A

D

R

I

I

V

I

K

A

E

K

R

H

P

P

D

R

L

L

I

L

L

V

L

L

T

S

D
|
D

D

E

V
x
I

Y
|
Y

G

E

-

H

-

I

T

I

F

Y

A

A

D

P

D

A

F

T

V

H

S

T

L

S

F

F

A

A

L

S

A

L

P

P

-

D

-

M

-

Y

K

E

N

R

T

T

I

L

L

T

V

V

Y

N

S

G

Y

F

S
|
S

K
|
K

Y

A

F

F

G

A

A

M

T

T

G

G

W

W

R
|
R

L

L

G

G

T

Y

I

L

A

A

binding pyridoxal-5'-phosphate: W123 (≠ F218), N173 (= N270), D202 (= D299), I204 (≠ V301), Y205 (= Y302), S235 (= S327), K236 (= K328), R244 (= R336)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 97, 98, 99

6f35A Crystal structure of the aspartate aminotranferase from rhizobium meliloti (see paper)
28% identity, 30% coverage: 179:345/554 of query aligns to 90:256/400 of 6f35A

query
sites
6f35A

E

E

F

L

D

D

V

E

F

I

A

T

V

V

E

-

G

-

G

A

T

T

A
x
G

A
|
A

M
x
K

T

Q

Y

I

I

L

F

F

N

N

T

A

M

M

R

M

E

A

N

S

H

-

L

-

I

L

K

D

A

P

G

G

D

D

T

E

I

V

A

I

L

I

G

P

M

T

P

P

I

Y

F

W

T

T

P

S

Y
|
Y

I

S

E

D

I

I

P

V

R

H

L

I

N

C

D

E

Y

G

R

K

L

-

N

-

V

P

V

V

N

L

L

I

E

A

A

C

D

D

V

A

A

S

N

S

G

G

W

F

Q

R

Y

L

S

T

K

A

K

E

E

K

L

L

D

E

K

A

L

A

R

I

D

T

P

P

K

R

V

T

K

R

A

W

F

V

F

L

L

L

V
x
N

N

S

P

P

S

S

N

N

P

P

P

S

S

G

V

A

K

A

M

Y

D

S

D

A

E

A

S

D

L

Y

R

R

Y

P

I

L

A

L

D

E

I

V

V

L

K

L

E

R

R

H

P

P

D

H

L

V

I

W

L

L

T

V

D
|
D

D

D

V
x
M

Y
|
Y

G

E

T

H

F

I

A

V

D

Y

D

D

-

G

-

F

-

R

F

F

V

V

S

T

L

P

F

A

A

Q

L

L

A

E

P

P

-

G

-

L

K

K

N

N

-

R

T

T

I

L

L

T

V

V

Y

N

S

G

Y

V

S
|
S

K
|
K

Y

A

F

Y

G

A

A

M

T

T

G

G

W

W

R
|
R

L

I

G

G

T

Y

I

A

A

G

T

G

H

P

R

R

D

E

binding pyridoxal-5'-phosphate: G99 (≠ A190), A100 (= A191), K101 (≠ M192), Y128 (= Y221), N171 (≠ V266), D204 (= D299), M206 (≠ V301), Y207 (= Y302), S238 (= S327), K239 (= K328), R247 (= R336)

P58350 Aspartate aminotransferase; AAT; AspAT; Putative 2-aminoadipate transaminase; Transaminase A; EC 2.6.1.1; EC 2.6.1.39 from Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) (see paper)
28% identity, 29% coverage: 179:338/554 of query aligns to 100:259/410 of P58350

query
sites
P58350

E

E

F

L

D

D

V

E

F

I

A

T

V

V

E

-

G

-

G

A

T

T

A

G

A

A

M

K

T

Q

Y

I

I

L

F

F

N

N

T

A

M

M

R

M

E

A

N

S

H

-

L

-

I

L

K

D

A

P

G

G

D

D

T

E

I

V

A

I

L

I

G

P

M

T

P

P

I

Y

F

W

T

T

P

S

Y

Y

I

S

E

D

I

I

P

V

R

H

L

I

N

C

D

E

Y

G

R

K

L

-

N

-

V

P

V

V

N

L

L

I

E

A

A

C

D

D

V

A

A

S

N

S

G

G

W

F

Q

R

Y

L

S

T

K

A

K

E

E

K

L

L

D

E

K

A

L

A

R

I

D

T

P

P

K

R

V

T

K

R

A

W

F

V

F

L

L

L

V

N

N

S

P

P

S

S

N

N

P

P

P

S

S

G

V

A

K

A

M

Y

D

S

D

A

E

A

S

D

L

Y

R

R

Y

P

I

L

A

L

D

E

I

V

V

L

K

L

E

R

R

H

P

P

D

H

L

V

I

W

L

L

T

V

D

D

V

M

Y

Y

G

E

T

H

F

I

A

V

D

Y

D

D

-

G

-

F

-

R

F

F

V

V

S

T

L

P

F

A

A

Q

L

L

A

E

P

P

-

G

-

L

K

K

N

N

-

R

T

T

I

L

L

T

V

V

Y

N

S

G

Y

V

S

S

K
|
K

Y

A

F

Y

G

A

A

M

T

T

G

G

W

W

R

R

L

I

G

G

K249 (= K328) modified: N6-(pyridoxal phosphate)lysine

1gdeA Crystal structure of pyrococcus protein a-1 e-form (see paper)
30% identity, 25% coverage: 202:341/554 of query aligns to 104:245/388 of 1gdeA

query
sites
1gdeA

N

S

H

A

L

F

I

L

K

K

A

D

G

G

D

E

T

E

I

V

A

L

L

I

G

P

M

T

P

P

I

A

F
|
F

T

V

P

S

Y

Y

-

A

-

P

-

A

-

V

-

I

-

L

-

A

-

G

-

K

-

P

I

V

E

E

I

V

P

P

R

T

L

Y

-

E

N

D

D

E

Y

F

R

R

L

L

N

N

V

V

V

-

N

-

L

-

E

-

A

-

D

-

V

-

A

-

N

-

G

-

W

-

Q

-

Y

-

S

-

K

-

K

D

E

E

L

L

D

K

K

K

L

Y

R

V

D

T

P

D

K

K

V

T

K

R

A

A

F

L

F

I

L

I

V
x
N

N

S

P

P

S

C

N
|
N

P

P

P

T

S

G

V

A

K

V

M

L

D

T

D

K

E

K

S

D

L

L

R

E

Y

E

I

I

A

A

D

D

I

F

V

V

K

V

E

E

R

H

P

-

D

D

L

L

I

I

L

V

L

I

T

S

D
|
D

D

E

V
|
V

Y
|
Y

G

E

T

H

F

F

A

I

D

Y

D

D

F

D

V

A

S

R

L

H

F

Y

A

S

L

I

A

A

P

S

-

L

-

D

-

G

-

M

-

F

K

E

N

R

T

T

I

I

L

T

V

V

Y

N

S

G

Y

F

S
|
S

K
|
K

Y

T

F

F

G

A

A

M

T

T

G

G

W

W

R
|
R

L

L

G

G

T

F

I

V

A

A

active site: K232 (= K328)
binding glutamic acid: F120 (= F218), N170 (= N270), K232 (= K328)
binding pyridoxal-5'-phosphate: F120 (= F218), N166 (≠ V266), N170 (= N270), D198 (= D299), V200 (= V301), Y201 (= Y302), S231 (= S327), K232 (= K328), R240 (= R336)

Sites not aligning to the query:

1gd9A Crystall structure of pyrococcus protein-a1 (see paper)
30% identity, 25% coverage: 202:341/554 of query aligns to 104:245/388 of 1gd9A

query
sites
1gd9A

N

S

H

A

L

F

I

L

K

K

A

D

G

G

D

E

T

E

I

V

A

L

L

I

G

P

M

T

P

P

I

A

F
|
F

T

V

P

S

Y

Y

-

A

-

P

-

A

-

V

-

I

-

L

-

A

-

G

-

K

-

P

I

V

E

E

I

V

P

P

R

T

L

Y

-

E

N

D

D

E

Y

F

R

R

L

L

N

N

V

V

V

-

N

-

L

-

E

-

A

-

D

-

V

-

A

-

N

-

G

-

W

-

Q

-

Y

-

S

-

K

-

K

D

E

E

L

L

D

K

K

K

L

Y

R

V

D

T

P

D

K

K

V

T

K

R

A

A

F

L

F

I

L

I

V
x
N

N

S

P

P

S

C

N
|
N

P

P

P

T

S

G

V

A

K

V

M

L

D

T

D

K

E

K

S

D

L

L

R

E

Y

E

I

I

A

A

D

D

I

F

V

V

K

V

E

E

R

H

P

-

D

D

L

L

I

I

L

V

L

I

T

S

D
|
D

D

E

V
|
V

Y
|
Y

G

E

T

H

F

F

A

I

D

Y

D

D

F

D

V

A

S

R

L

H

F

Y

A

S

L

I

A

A

P

S

-

L

-

D

-

G

-

M

-

F

K

E

N

R

T

T

I

I

L

T

V

V

Y

N

S

G

Y

F

S
|
S

K
|
K

Y

T

F

F

G

A

A

M

T

T

G

G

W

W

R
|
R

L

L

G

G

T

F

I

V

A

A

active site: K232 (= K328)
binding pyridoxal-5'-phosphate: F120 (= F218), N166 (≠ V266), N170 (= N270), D198 (= D299), V200 (= V301), Y201 (= Y302), S231 (= S327), K232 (= K328), R240 (= R336)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 94, 95, 96

Q02635 Aspartate/prephenate aminotransferase; AspAT / PAT; Transaminase A; EC 2.6.1.1; EC 2.6.1.79 from Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) (see paper)
28% identity, 28% coverage: 188:341/554 of query aligns to 97:252/400 of Q02635

query
sites
Q02635

G

G

T

T

A

G

M

K

T

Q

Y

I

I

L

F

F

N

N

T

A

M

F

R

M

E

A

N

T

H

-

L

-

I

L

K

N

A

P

G

G

D

D

T

E

I

V

A

V

L

I

G

P

M

A

P

P

I

Y

F

W

T

V

P

S

Y

Y

I

P

E

E

I

M

P

V

R

A

L

L

N

C

D

G

Y

G

R

T

L

P

N

V

V

F

V

V

N

P

L

T

E

R

A

Q

D

E

V

-

A

-

N

N

G

N

W

F

Q

K

Y

L

S

K

K

A

K

E

E

D

L

L

D

D

K

R

L

A

R

I

D

T

P

P

K

K

V

T

K

K

A

W

F

F

F

V

L

F

V

N

N

S

P

P

S

S

N

N

P

P

P

S

S

G

V

A

K

A

M

Y

D

S

D

H

E

E

S

E

L

L

R

K

Y

A

I

L

A

T

D

D

I

V

V

L

K

M

E

K

R

H

P

P

D

H

L

V

I

W

L

V

L

L

T

T

D

D

V

M

Y

Y

G

E

-

H

-

L

T

T

F

Y

A

G

D

D

-

F

D

R

F

F

V

A

S

T

L

P

F

V

A

E

L

V

A

E

P

P

-

G

-

L

-

Y

K

E

N

R

T

T

I

L

L

T

V

M

Y

N

S

G

Y

V

S

S

K

K

Y

A

F

Y

G

A

A

M

T

T

G

G

W

W

R

R

L

I

G

G

T

Y

I

A

A

A

Sites not aligning to the query:

12 Important for prephenate aminotransferase activity; K→G: 7-fold increase in Km for prephenate. Weakly affects Km for oxaloacetate.

6f77A Crystal structure of the prephenate aminotransferase from rhizobium meliloti (see paper)
28% identity, 28% coverage: 188:341/554 of query aligns to 96:251/399 of 6f77A

query
sites
6f77A

G

G

T

T

A
x
G

M
x
K

T

Q

Y

I

I

L

F

F

N

N

T

A

M

F

R

M

E

A

N

T

H

-

L

-

I

L

K

N

A

P

G

G

D

D

T

E

I

V

A

V

L

I

G

P

M

A

P

P

I

Y

F
x
W

T

V

P

S

Y
|
Y

I

P

E

E

I

M

P

V

R

A

L

L

N

C

D

G

Y

G

R

T

L

P

N

V

V

F

V

V

N

P

L

T

E

R

A

Q

D

E

V

-

A

-

N

N

G

N

W

F

Q

K

Y

L

S

K

K

A

K

E

E

D

L

L

D

D

K

R

L

A

R

I

D

T

P

P

K

K

V

T

K

K

A

W

F

F

F

V

L

F

V
x
N

N

S

P

P

S

S

N
|
N

P

P

P

S

S

G

V

A

K

A

M

Y

D

S

D

H

E

E

S

E

L

L

R

K

Y

A

I

L

A

T

D

D

I

V

V

L

K

M

E

K

R

H

P

P

D

H

L

V

I

W

L

V

L

L

T

T

D
|
D

D

D

V
x
M

Y
|
Y

G

E

-

H

-

L

T

T

F

Y

A

G

D

D

-

F

D

R

F

F

V

A

S

T

L

P

F

V

A

E

L

V

A

E

P

P

-

G

-

L

-

Y

K

E

N

R

T

T

I

L

L

T

V

M

Y

N

S

G

Y

V

S
|
S

K
|
K

Y

A

F

Y

G

A

A

M

T

T

G

G

W

W

R
|
R

L

I

G

G

T

Y

I

A

A

A

binding pyridoxal-5'-phosphate: G98 (≠ A190), G99 (≠ A191), K100 (≠ M192), W124 (≠ F218), Y127 (= Y221), N170 (≠ V266), N174 (= N270), D203 (= D299), M205 (≠ V301), Y206 (= Y302), S237 (= S327), K238 (= K328), R246 (= R336)

5wmlA Arabidopsis thaliana prephenate aminotransferase mutant- k306a (see paper)
29% identity, 25% coverage: 204:341/554 of query aligns to 110:250/404 of 5wmlA

query
sites
5wmlA

L

V

I

C

K

S

A

P

G

G

D

D

T

E

I

V

A

I

L

I

G

P

M

A

P

P

I

Y

F
x
W

T

V

P

S

Y

Y

I

T

E

E

I

Q

P

A

R

R

L

L

N

A

D

D

Y

-

R

-

L

A

N

T

V

P

V

V

N

V

L

I

E

P

A

T

D

K

V

I

A

S

N

N

G

N

W

F

Q

L

Y

L

S

D

K

P

K

K

E

D

L

L

D

E

K

S

L

K

R

L

D

T

P

E

K

K

V

S

K

R

A

L

F

L

F

I

L

L

V

C

N

S

P

P

S

S

N
|
N

P

P

P

T

S

G

V

S

K

V

M

Y

D

P

D

K

E

S

S

L

L

L

R

E

Y

E

I

I

A

A

D

R

I

I

V

I

K

A

E

K

R

H

P

P

D

R

L

L

I

L

L

V

L

L

T

S

D
|
D

D

E

V
x
I

Y
|
Y

G

E

-

H

-

I

T

I

F

Y

A

A

D

P

D

A

F

T

V

H

S

T

L

S

F

F

A

A

L

S

A

L

P

P

-

D

-

M

-

Y

K

E

N

R

T

T

I

L

L

T

V

V

Y

N

S

G

Y

F

S
|
S

K

A

Y

A

F

F

G

A

A

M

T

T

G

G

W

W

R
|
R

L

L

G

G

T

Y

I

L

A

A

binding glutamic acid: W124 (≠ F218), N174 (= N270)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: W124 (≠ F218), N174 (= N270), D203 (= D299), I205 (≠ V301), Y206 (= Y302), S236 (= S327), R245 (= R336)

Sites not aligning to the query:

binding glutamic acid: 15, 16, 36, 37, 38, 100, 326, 376
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: 98, 99, 100, 103

1gc4A Thermus thermophilus aspartate aminotransferase tetra mutant 2 complexed with aspartate (see paper)
24% identity, 42% coverage: 146:377/554 of query aligns to 62:270/382 of 1gc4A

query
sites
1gc4A

S

T

N

K

Y
|
Y

P

A

V

P

P

P

D

A

R

G

M

I

L

P

K

E

L

L

S

R

E

E

L

A

I

L

V

A

G

E

Q

K

Y

F

L

R

R

R

K

E

E

N

M

G

I

L

G

S

D

V

H

T

P

P

F

-

V

-

G

-

E

E

F

E

D

T

V

I

F

V

A

T

V

V

E

-

G
|
G

T
x
S

A

Q

A

A

M

L

T

F

Y

N

I

L

F

F

N

Q

T

A

M

-

R

-

E

-

N

-

H

-

L

I

I

L

K

D

A

P

G

G

D

D

T

E

I

V

A

I

L

V

G

L

M

S

P

P

I

Y

F
x
W

T

V

P

S

Y
|
Y

I

P

E

E

I

M

P

V

R

R

L

F

N

A

D

G

Y

G

R

-

L

-

N

V

V

V

N

E

L

V

E

E

A

T

D

L

V

P

A

E

N

E

G

G

W

F

Q

V

Y

P

S

D

K

P

K

E

E

R

L

V

D

R

K

R

L

A

R

I

D

T

P

P

K

R

V

T

K

K

A

A

F

L

F

V

L

V

V
x
N

N

S

P

P

S

N

N
|
N

P

P

P

T

S

G

V

A

K

V

M

Y

D

P

D

K

E

E

S

V

L

L

R

E

Y

A

I

L

A

A

D

R

I

L

V

A

K

V

E

E

R

H

P

-

D

D

L

F

I

Y

L

L

L

V

T

S

D
|
D

D

E

V
x
I

Y
|
Y

G

E

-

H

-

L

T

L

F

Y

A

E

D

G

D

E

F

H

V

F

S

S

L

P

F

G

A

R

L

V

A

A

P

P

K

E

N

H

T

T

I

L

L

T

V

V

Y

N

S

G

Y

A

S

A

K
|
K

Y

A

F

F

G

A

A

M

T

T

G

G

W

W

R
|
R

L

I

G

G

T

-

I

-

A

-

T

-

H

-

R

-

D

-

N

-

V

-

L

-

D

-

K

-

L

-

I

Y

A

A

D

C

L

G

P

P

K

K

D

E

A

V

K

I

K

K

Q

A

L

M

H

A

E

S

R

V

Y

S

E
x
R

S

Q

I

S

T

T

E

S

P

P

D

D

K

T

L

I

binding aspartic acid: Y64 (= Y148), W125 (≠ F218), N175 (= N270), Y206 (= Y302), K234 (= K328), R261 (≠ E368)
binding pyridoxal-5'-phosphate: G99 (= G187), G100 (= G188), S101 (≠ T189), W125 (≠ F218), Y128 (= Y221), N171 (≠ V266), N175 (= N270), D203 (= D299), I205 (≠ V301), Y206 (= Y302), K234 (= K328), R242 (= R336)

Sites not aligning to the query:

binding aspartic acid: 39, 322, 361

1gc3A Thermus thermophilus aspartate aminotransferase tetra mutant 2 complexed with tryptophan (see paper)
24% identity, 42% coverage: 146:377/554 of query aligns to 62:270/382 of 1gc3A

query
sites
1gc3A

S

T

N

K

Y

Y

P

A

V

P

P

P

D

A

R

G

M

I

L

P

K

E

L

L

S

R

E

E

L

A

I

L

V

A

G

E

Q

K

Y

F

L

R

R

R

K

E

E

N

M

G

I

L

G

S

D

V

H

T

P

P

F

-

V

-

G

-

E

E

F

E

D

T

V

I

F

V

A

T

V

V

E

-

G
|
G

T
x
S

A

Q

A

A

M

L

T

F

Y

N

I

L

F

F

N

Q

T

A

M

-

R

-

E

-

N

-

H

-

L

I

I

L

K

D

A

P

G

G

D

D

T

E

I

V

A

I

L

V

G

L

M

S

P

P

I

Y

F
x
W

T

V

P

S

Y

Y

I

P

E

E

I

M

P

V

R

R

L

F

N

A

D

G

Y

G

R

-

L

-

N

V

V

V

N

E

L

V

E

E

A

T

D

L

V

P

A

E

N

E

G

G

W

F

Q

V

Y

P

S

D

K

P

K

E

E

R

L

V

D

R

K

R

L

A

R

I

D

T

P

P

K

R

V

T

K

K

A

A

F

L

F

V

L

V

V

N

N

S

P

P

S

N

N
|
N

P

P

P

T

S

G

V

A

K

V

M

Y

D

P

D

K

E

E

S

V

L

L

R

E

Y

A

I

L

A

A

D

R

I

L

V

A

K

V

E

E

R

H

P

-

D

D

L

F

I

Y

L

L

L

V

T

S

D
|
D

D

E

V
x
I

Y
|
Y

G

E

-

H

-

L

T

L

F

Y

A

E

D

G

D

E

F

H

V

F

S

S

L

P

F

G

A

R

L

V

A

A

P

P

K

E

N

H

T

T

I

L

L

T

V

V

Y

N

S

G

Y

A

S
x
A

K
|
K

Y

A

F

F

G

A

A

M

T

T

G

G

W

W

R
|
R

L

I

G

G

T

-

I

-

A

-

T

-

H

-

R

-

D

-

N

-

V

-

L

-

D

-

K

-

L

-

I

Y

A

A

D

C

L

G

P

P

K

K

D

E

A

V

K

I

K

K

Q

A

L

M

H

A

E

S

R

V

Y

S

E

R

S

Q

I

S

T

T

E

S

P

P

D

D

K

T

L

I

binding pyridoxal-5'-phosphate: G99 (= G187), G100 (= G188), S101 (≠ T189), W125 (≠ F218), N175 (= N270), D203 (= D299), I205 (≠ V301), Y206 (= Y302), A233 (≠ S327), K234 (= K328), R242 (= R336)
binding tryptophan: W125 (≠ F218), N175 (= N270), K234 (= K328)

Sites not aligning to the query:

binding tryptophan: 16, 20, 37, 38, 39, 40, 322, 361

1b5oA Thermus thermophilus aspartate aminotransferase single mutant 1 (see paper)
24% identity, 42% coverage: 146:377/554 of query aligns to 62:270/382 of 1b5oA

query
sites
1b5oA

S

T

N

K

Y

Y

P

A

V

P

P

P

D

A

R

G

M

I

L

P

K

E

L

L

S

R

E

E

L

A

I

L

V

A

G

E

Q

K

Y

F

L

R

R

R

K

E

E

N

M

G

I

L

G

S

D

V

H

T

P

P

F

-

V

-

G

-

E

E

F

E

D

T

V

I

F

V

A

T

V

V

E

-

G
|
G

T
x
S

A

Q

A

A

M

L

T

F

Y

N

I

L

F

F

N

Q

T

A

M

-

R

-

E

-

N

-

H

-

L

I

I

L

K

D

A

P

G

G

D

D

T

E

I

V

A

I

L

V

G

L

M

S

P

P

I

Y

F
x
W

T

V

P

S

Y

Y

I

P

E

E

I

M

P

V

R

R

L

F

N

A

D

G

Y

G

R

-

L

-

N

V

V

V

N

E

L

V

E

E

A

T

D

L

V

P

A

E

N

E

G

G

W

F

Q

V

Y

P

S

D

K

P

K

E

E

R

L

V

D

R

K

R

L

A

R

I

D

T

P

P

K

R

V

T

K

K

A

A

F

L

F

V

L

V

V
x
N

N

S

P

P

S

N

N
|
N

P

P

P

T

S

G

V

A

K

V

M

Y

D

P

D

K

E

E

S

V

L

L

R

E

Y

A

I

L

A

A

D

R

I

L

V

A

K

V

E

E

R

H

P

-

D

D

L

F

I

Y

L

L

L

V

T

S

D
|
D

D

E

V
x
I

Y
|
Y

G

E

-

H

-

L

T

L

F

Y

A

E

D

G

D

E

F

H

V

F

S

S

L

P

F

G

A

R

L

V

A

A

P

P

K

E

N

H

T

T

I

L

L

T

V

V

Y

N

S

G

Y

A

S
x
A

K
|
K

Y

A

F

F

G

A

A

M

T

T

G

G

W

W

R
|
R

L

I

G

G

T

-

I

-

A

-

T

-

H

-

R

-

D

-

N

-

V

-

L

-

D

-

K

-

L

-

I

Y

A

A

D

C

L

G

P

P

K

K

D

E

A

V

K

I

K

K

Q

A

L

M

H

A

E

S

R

V

Y

S

E

S

S

Q

I

S

T

T

E

S

P

P

D

D

K

T

L

I

active site: W125 (≠ F218), D203 (= D299), I205 (≠ V301), K234 (= K328)
binding pyridoxal-5'-phosphate: G99 (= G187), G100 (= G188), S101 (≠ T189), W125 (≠ F218), N171 (≠ V266), N175 (= N270), D203 (= D299), I205 (≠ V301), Y206 (= Y302), A233 (≠ S327), K234 (= K328), R242 (= R336)

1w7nA Crystal structure of human kynurenine aminotransferase i in pmp form (see paper)
25% identity, 34% coverage: 181:368/554 of query aligns to 90:287/415 of 1w7nA

query
sites
1w7nA

D

N

V

V

F

L

A

V

V

T

E

V

G
|
G

T
x
Y

A

G

A

A

M

L

T

F

Y

T

I

A

F

F

N

Q

T

A

M

-

R

-

E

-

N

-

H

-

L

L

I

V

K

D

A

E

G

G

D

D

T

E

I

V

A

I

L

I

G

I

M

E

P

P

I

F

F
|
F

T

D

P

C

Y

Y

I

E

E

P

I

M

P

T

R

M

L

M

N

A

D

G

Y

G

R

R

L

P

N

V

V

F

V

V

N

S

L

L

-

K

-

P

-

G

-

P

-

G

E

E

A

L

D

G

V

S

A

S

N

S

G

N

W

W

Q

Q

Y

L

S

D

K

P

K

M

E

E

L

L

D

A

K

G

L

K

R

F

D

T

P

S

K

R

V

T

K

K

A

A

F

L

F

V

L

L

V
x
N

N

T

P

P

S

N

N
|
N

P

P

P

L

S

G

V

K

K

V

M

F

D

S

D

R

E

E

S

E

L

L

R

E

Y

L

I

V

A

A

D

S

I

L

V

C

K

Q

E

Q

R

H

P

-

D

D

L

V

I

V

L

C

L

I

T

T

D
|
D

D

E

V
|
V

Y
|
Y

G

Q

T

W

F

M

A

V

D

Y

D

D

F

G

V

H

S

Q

L

H

F

I

A

S

L

I

A

A

P

S

-

L

-

P

-

G

-

M

-

W

K

E

N

R

T

T

I

L

L

T

V

I

Y

G

S
|
S

Y

A

S

G

K
|
K

Y

T

F

F

G

S

A

A

T

T

G

G

W

W

R
x
K

L

V

G

G

-

W

-

V

-

L

-

G

-

P

-

D

T

H

I

I

A

M

T

K

H

H

R

L

D

R

N

T

V

V

L

H

D

Q

K

N

L

S

I

V

A

F

D

H

L

C

P

P

K

T

D

Q

A

S

K

Q

K

A

Q

A

L

V

H

A

E

E

R

S

Y

F

E

E

active site: F122 (= F218), D207 (= D299), V209 (= V301), K241 (= K328)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G96 (= G187), G97 (= G188), Y98 (≠ T189), F122 (= F218), N175 (≠ V266), N179 (= N270), D207 (= D299), V209 (= V301), Y210 (= Y302), S238 (= S325), K241 (= K328), K249 (≠ R336)

1w7mA Crystal structure of human kynurenine aminotransferase i in complex with l-phe (see paper)
25% identity, 34% coverage: 181:368/554 of query aligns to 90:287/415 of 1w7mA

query
sites
1w7mA

D

N

V

V

F

L

A

V

V

T

E

V

G
|
G

T
x
Y

A

G

A

A

M

L

T

F

Y

T

I

A

F

F

N

Q

T

A

M

-

R

-

E

-

N

-