SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS05360 FitnessBrowser__BFirm:BPHYT_RS05360 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P53322 High-affinity nicotinic acid transporter; Nicotinic acid permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
23% identity, 76% coverage: 5:337/439 of query aligns to 65:399/534 of P53322

query
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P53322

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K283 (= K225) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
23% identity, 81% coverage: 73:428/439 of query aligns to 113:473/495 of Q9NRA2

query
sites
Q9NRA2

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K136 (≠ R101) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
H183 (≠ I141) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
LL 198:199 (≠ MT 156:157) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
IL 266:267 (≠ LE 231:232) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
SSLRN 268:272 (≠ RHVSA 233:237) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
G328 (= G293) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
P334 (= P299) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
G371 (= G331) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794

P0AA76 D-galactonate transporter; D-galactonate/H(+) symporter from Escherichia coli (strain K12) (see paper)
21% identity, 85% coverage: 35:406/439 of query aligns to 20:397/430 of P0AA76

query
sites
P0AA76

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Y29 (= Y44) binding
D31 (= D46) mutation to N: Loss of galactonate transport activity.
R32 (= R47) binding
Y64 (= Y79) binding
E118 (= E133) mutation to Q: Loss of galactonate transport activity.
W358 (= W367) binding

Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
24% identity, 56% coverage: 73:320/439 of query aligns to 113:355/495 of Q8BN82

query
sites
Q8BN82

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A

G

E

E

A

G

G

V

F

T

F

F

P

P

G

A

I

M

I
x
H

L

A

Y

M

L

W

T

S

Y

S

W

W

Y

A

P

P

A

P

H

L

R

E

R

R

G

S

R

K

M

L

T

L

T

T

W

I

F

S

M

Y

T

A

A

G

I

A

A

Q

L

L

S

G

G

T

V

V

I

I

G

S

G

L

P

P

V

L

S

S

G

G

Y

I

I

I

L

C

K

Y

T

Y

F

M

N

N

G

-

M

-

N

-

G

-

W

-

H

-

G

-

W

W

Q

T

W

Y

L

V

F

F

L

Y

L

L

E

F

G

G

I

I

P

V

S

G

V

I

I

V

V

W

G

F

I

I

M

L

V

W

F

M

T

W

M

I

L

V

D

S

D

D

R

T

I

P

S

E

K

T

A

H

K

K

W

T

L

I

T

S

K

H

E

Y

E

E

Q

K

Q

E

L

Y

L

I

E

V

R

S

H

S

V

L

S

K

A

N

E

Q

E

L

A

S

T

S

K

Q

H

K

D

V

M

V

P

P

I

W

R

G

Q

S

V

I

L

L

T

K

S

S

G

L

R

P

V

L

L

W

M

A

L

I

S

V

L

V

T

A

Y

H

F

F

S

S

F

Y

V

N

M

W

G

S

L

F

Y

Y

G

T

V

L

S

L

F

T

W

L

L

L

P

P

T

T

I

Y

I

M

K

K

A

E

T

I

G

L

V

R

T

F

D

N

A

V

F

Q

M

E

I

N

G

G

L

F

L

L

S

S

A

A

I

L

P

P

F

Y

A

F

G

G

A

C

V

W

V

L

A

C

M

M

V

I

F

L

V

C

S

G

R

Q

S

A

A

A

D

D

R

Y

K

L

R

R

E

V

R

K

H183 (≠ I141) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.

Sites not aligning to the query:

39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.

6e9nA E. Coli d-galactonate:proton symporter in the inward open form (see paper)
21% identity, 85% coverage: 35:407/439 of query aligns to 9:379/409 of 6e9nA

query
sites
6e9nA

L

M

L

I

M

F

L

I

C

T

Y

V

V

V

V

I

A

C

Y

Y

L

V

D

D

R

R

V

A

N

N

V

L

G

A

F

V

A

A

K

S

L

A

Q

H

M

I

T

Q

S

E

D

E

L

F

G

G

L

I

S

T

D

K

A

A

V

E

Y

M

G

G

F

Y

G

V

A

F

G

S

I

A

F

F

F

A

V

W

G

L

Y

Y

F

T

I

L

F

C

E
x
Q

I

I

P

P

S

G

N

G

V

W

I

F

L

L

H

D

K

R

V

V

G

G

A

S

R

R

V

V

W

T

I

Y

A

F

R

I

I

A

M

I

V

F

S

G

W

W

G

S

V

V

I

A

S

T

M

L

L

F

T

Q

M

G

F

F

V

A

T

T

T

G

P

L

T

M

M

S

F

L

Y

I

V

G

M

L

R

R

F

A

L

I

L

T

G

G

L

I

A

F

E

E

A

A

G

P

F

A

F
|
F

P

P

G

T

I

N

L

R

Y

M

L

V

T

T

Y

S

W

W

Y

F

P

P

A

E

H

H

R

E

R

R

G

A

R

S

M

A

T

V

T

G

W

F

F

Y

M

T

T

S

A

G

I

Q

A

F

L

V

S

G

G

L

V

A

I

F

G

L

G

T

P

P

V

L

S

L

G

I

Y

W

I

I

L

Q

K

E

T

M

F

L

N

S

G

-

M

-

N

-

G

-

W

-

H

-

G

-

W

W

Q

H

W

W

L

V

F

F

L

I

L

V

E

T

G

G

I

G

P

I

S

G

V

I

I

I

V

W

G

S

I

L

M

I

V

W

F

F

T

K

M

V

L

Y

D

Q

-

P

D

P

R

R

I

L

S

T

K

K

A

G

K

-

W

-

L

I

T

S

K

K

E

A

E

E

Q

-

L

-

L

L

E

D

R

Y

H

I

V

R

S

D

A

G

E

G

A

L

T

V

K

D

H

G

D

D

M

A

P

P

I

L

R

T

-

A

-

K

-

D

-

W

Q

K

V

L

L

V

T

F

S

H

G

R

R

K

V

L

L

I

M

G

L

V

S

Y

L

L

T

G

Y

Q

F

F

S

A

F

V

V

A

M

S

G

T

L

L

Y

W

G

F

V

F

S

L

F

T

W

W

L

F

P

P

T

N

I

Y

I

L

K

T

A

Q

T

E

G

K

V

G

T

I

D

T

A

A

F

L

M

K

I

A

G

G

L

F

L

M

S

T

A

T

I

V

P

P

F

F

A

L

G

A

A

A

V

F

V

V

A

G

M

V

V

L

F

L

V

S

S

G

R

W

S

V

A

A

D

D

R

L

K

L

R

V

E

R

R

K

R

G

W

F

H

S

I

L

A

G

L

F

-

A

-

R

-

K

-

T

P

P

A

I

F

I

A

C

G

G

A

L

V

L

-

I

-

S

G

T

L

C

V

I

L

M

S

G

V

A

V

N

W

Y

A

T

H

N

N

D

T

P

V

M

L

M

A

I

M

M

A

C

S

L

L

M

T

A

L

L

A

A

T

F

M

F

G

G

I

N

L

-

T

G

T

F

L

A

P

S

L

I

F

T

W

W

S

S

L

L

-

V

-

S

-

L

-

A

P

P

T

M

A

R

I

L

L

I

A

G

G

L

T

T

G

G

A

-

G

-

I

-

A

G

M

V

I

F

N
|
N

S

F

I

A

G

G

N

G

L

L

A

G

G

G

F

I

L

T

S

V

P

P

Y

L

A

V

V

V

G

G

W

Y

L

L

K

A

Q

Q

A

G

binding D-gluconic acid: Q57 (≠ E83), F111 (= F137), N359 (= N387)

Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
23% identity, 56% coverage: 73:320/439 of query aligns to 113:355/495 of Q5Q0U0

query
sites
Q5Q0U0

G

G

I

S

F

F

V

Y

G

G

Y

Y

F

I

I

V

F

T

E

Q

I

I

P

P

S

G

N

G

V

Y

I

M

L

A

H

S

K

R

V

I

G

G

A

G

R
x
K

V

L

W

L

I

L

A

G

R

L

I

G

M

I

V

L

S

G

W

T

G

A

V

V

I

F

S

T

M

L

L

F

T

T

M

P

F

L

V

A

T

A

T

D

-

L

-

G

P

V

T

V

M

A

F

L

Y

I

V

V

M

L

R
|
R

F

A

L

L

L
x
E

G
|
G

L

L

A

G

E
|
E

A
x
G

G

V

F

T

F
|
F

P
|
P

G

A

I

M

I
x
H

L

A

Y

M

L
x
W

T

S

Y

S

W

W

Y

A

P

P

A

P

H

L

R

E

R

R

G

S

R

K

M

L

T

L

T

T

W

I

F

S

M

Y

T

A

A

G

I

A

A

Q

L

L

S

G

G

T

V

V

I

I

G

S

G

L

P

P

V

L

S

S

G

G

Y

I

I

I

L

C

K

Y

T

Y

F

M

N

N

G

-

M

-

N

-

G

-

W

-

H

-

G

-

W

W

Q

T

W

Y

L

V

F

F

L

Y

L

L

E

F

G

G

I

L

P

V

S

G

V

I

I

V

V

W

G

F

I

I

M

L

V

W

F

M

T

W

M

I

L

V

D

S

D

D

R

T

I

P

S

E

K

T

A

H

K

K

W

T

L

I

T

S

K

H

E

Y

E

E

Q

K

Q

E

L

Y

L

I

E

V

R
x
S

H
x
S

V
x
L

S
x
K

A
x
N

E

Q

E

L

A

S

T

S

K

Q

H

K

D

V

M

V

P

P

I

W

R

V

Q

S

V

I

L

L

T

K

S

S

G

L

R

P

V

L

L

W

M

A

L

I

S

V

L

V

T

A

Y

H

F

F

S

S

F

Y

V

N

M

W

G

T

L

F

Y

Y

G

T

V

L

S

L

F

T

W

L

L

L

P

P

T

T

I

Y

I

M

K

K

A

E

T

I

G

L

V

R

T

F

D

N

A

V

F

Q

M

E

I

N

G

G

L

F

L

L

S

S

A

A

I

L

P
|
P

F

Y

A

F

G

G

A

C

V

W

V

L

A

C

M

M

V

I

F

V

V

C

S

G

R

Q

S

A

A

A

D

D

R

Y

K

L

R

R

E

V

R

K

K136 (≠ R96) mutation to E: Markedly decreases H(+)-coupled sialic acid transporter activity.
R168 (= R126) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
E171 (≠ L129) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
G172 (= G130) mutation to C: Decreases protein levels and alters subcellular localization.
E175 (= E133) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
G176 (≠ A134) mutation to C: Decreases protein levels and alters subcellular localization.
F179 (= F137) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
P180 (= P138) mutation to C: Decreases protein levels and alters subcellular localization.
H183 (≠ I141) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
W186 (≠ L144) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
SSLKN 268:272 (≠ RHVSA 233:237) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
P334 (= P299) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.
371 G→V: Remains in the endoplasmic reticulum.

Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
27% identity, 46% coverage: 91:293/439 of query aligns to 82:301/452 of Q5EXK5

query
sites
Q5EXK5

H
x
D

K

R

V

F

G

G

A

R

R

K

V

R

W

V

I

I

A

E

R

F

I

C

M

V

V

A

S

L

W

F

G

G

V

L

I

F

S

S

M

L

L

L

T

S

M

A

F

F

V

A

T

P

T

D

P

L

T

Q

M

M

F

L

Y

V

V

F

M

L

R

R

F

F

L

L

L

T

G

G

L

L

A

G

E

L

A

G

G

G

F

A

F

M

P

P

G

N

I

T

I

I

L

T

Y

M

L

T

T

S

Y

E

W

Y

Y

L

P

P

A

A

H

R

R

R

G

G

R

A

M

L

T

V

T

T

W

L

F

M

M

F

T

C

A

G

I

F

A

T

L

L

S

G

G

S

V

A

I

F

G

G

P

V

V

V

S

S

G

A

Y

Q

I

L

L

V

K

P

T

-

F

-

N

-

G

-

M

L

N

I

G

G

W

W

H

H

G

G

W

-

Q

-

W

-

L

I

F

L

L

V

L

L

E

G

G

G

I

V

P

L

S

P

V

L

I

I

V

L

G

A

I

V

M

A

V

L

F

V

T

P

M

L

L

L

D

P

D

E

R

-

I

-

S

-

K

S

A

P

K

R

W

W

L

Q

T

I

K

R

E

R

E

Q

-

L

-

P

Q

Q

Q

A

L

T

L

I

E

A

R

R

H

T

V

V

S

G

A

A

E

I

E

T

A

G

T

E

K

R

H

Y

-

N

-

D

-

T

-

H

-

F

-

W

-

L

D

D

M

E

P

P

-

A

-

G

-

A

-

K

-

G

-

S

I

I

R

S

Q

Q

V

-

L

L

T

F

S

A

G

G

R

R

-

Q

-

L

-

A

-

I

V

T

L

L

M

M

L

L

S

W

L

V

T

V

Y

F

F

F

S

M

F

S

V

L

M

L

G

I

L

I

Y

Y

G

L

V

L

S

S

F

S

W

W

L

M

P

P

T

T

I

L

K

N

A

H

T

R

G

G

-

I

-

D

-

L

-

Q

-

H

-

A

-

S

-

W

V

V

T

T

D

A

A

A

F

F

M

Q

I

I

G

G

D82 (≠ H91) mutation to A: Loss of activity.

Sites not aligning to the query:

311 V→W: Loss of activity.
314 D→A: Loss of activity.

6e9oA E. Coli d-galactonate:proton symporter mutant e133q in the outward substrate-bound form (see paper)
21% identity, 85% coverage: 35:407/439 of query aligns to 12:363/393 of 6e9oA

query
sites
6e9oA

L

M

L

I

M

F

L

I

C

T

Y

V

V

V

V

I

A

C

Y
|
Y

L

V

D

D

R
|
R

V

A

N

N

V

L

G

A

F

V

A

A

K

S

L

A

Q

H

M

I

T

Q

S

E

D

E

L

F

G

G

L

I

S

T

D

K

A

A

V

E

Y

M

G

G

F

Y

G

V

A

F

G

S

I

A

F

F

F

A

V

W

G

L

Y
|
Y

F

T

I

L

F

C

E

Q

I

I

P

P

S

G

N

G

V

W

I

F

L

L

H

D

K

R

V

V

G

G

A

S

R

R

V

V

W

T

I

Y

A

F

R

I

I

A

M

I

V

F

S

G

W

W

G

S

V

V

I

A

S

T

M

L

L

F

T

Q

M

G

F

F

V

A

T

T

T

G

P

L

T

M

M

S

F

L

Y

I

V

G

M

L

R

R

F

A

L

I

L

T

G

G

L

I

A

F

E

Q

A

A

G

P

F

A

F
|
F

P

P

G

T

I

N

L

R

Y

M

L

V

T

T

Y

S

W

W

Y

F

P

P

A

E

H

H

R

E

R

R

G

A

R

S

M

A

T

V

T

G

W

F

F

Y

M

T

T

S

A

G

I
x
Q

A

F

L

V

S

G

G

L

V

A

I

F

G

L

G

T

P

P

V

L

S

L

G

I

Y

W

I

I

L

Q

K

E

T

M

F

L

N

S

G

-

M

-

N

-

G

-

W

-

H

-

G

-

W

W

Q

H

W

W

L

V

F

F

L

I

L

V

E

T

G

G

I

G

P

I

S

G

V

I

I

I

V

W

G

S

I

L

M

I

V

W

F

F

T

K

M

V

L

Y

D

Q

-

P

D

P

R

R

I

L

S

T

K

K

A

G

K

-

W

-

L

I

T

S

K

K

E

A

E

E

Q

L

Q

D

L

Y

L

I

E

-

R

R

H

D

V

G

S

G

A

G

E

L

E

V

A

L

T

T

K

A

H

K

D

D

M

W

P

K

I

-

R

-

Q

-

V

-

L

-

T

-

S

-

G

-

R

-

V

-

L

L

M

V

L

F

S

H

L

R

T

K

Y

L

F

I

S

G

F

V

V

Y

M

L

G

G

L
x
Q

Y

F

G

A

V

V

-
x
A

-

S

S

T

F

L

W

W

L

F

P

F

T

L

I

T

I

L

K

K

A

A

T

-

G

-

V

-

T

-

D

-

A

-

F

-

M

-

I

-

G

G

L

F

L

M

S

T

A

T

I

V

P

P

F

F

A

L

G

A

A

A

V

F

V

V

A

G

M

V

V

L

F

L

V

S

S

G

R

W

S

V

A

A

D

D

R

L

K

L

R

V

E

R

R

K

R

G

W

F

H

S

I

L

A

G

L

F

-

A

-

R

-

K

-

T

P

P

A

I

F

I

A

C

G

G

A

L

V

L

-

I

-

S

G

T

L

C

V

I

L

M

S

G

V

A

V

N

W

Y

A

T

H

N

N

D

T

P

V

M

L

M

A

I

M

M

A

C

S

L

L

M

T

A

L

L

A

A

T

F

M

F

G

G

I

N

L

-

T

G

T

F

L

A

P
x
S

L

I

F

T

W

W

S

S

L

L

-

V

-

S

-

L

-

A

P

P

T

M

A

R

I

L

L

I

A

G

G

L

T

T

G

G

A

-

G

-

I

-

A

G

M

V

I

F

N
|
N

S

F

I

A

G

G

N

G

L

L

A

G

G

G

F

I

L

T

S

V

P

P

Y

L

A

V

V

V

G

G

W

Y

L

L

K

A

Q

Q

A

G

binding D-galactonic acid: Y21 (= Y44), R24 (= R47), Y56 (= Y79), F114 (= F137), Q141 (≠ I164), Q228 (≠ L270), A232 (vs. gap), S320 (≠ P364), N343 (= N387)

Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
21% identity, 97% coverage: 1:428/439 of query aligns to 43:492/582 of Q9JI12

query
sites
Q9JI12

M

L

A

T

S

E

P

D

A

G

N

K

P

P

L

L

H

E

H

V

P

P

G

E

A

K

G

K

A

A

P

P

P

-

S

-

T

-

F

L

E

C

E

D

A

C

T

T

Y

C

R

F

K

G

V

L

S

P

W

R

R

R

L

Y

A

I

P

I

L

A

L

I

M

M

-

S

-

G

L

L

C

G

Y

F

V

C

V

I

A

S

Y

F

L

G

D

I

R
|
R

V

C

N

N

V

L

G

G

F

V

A

A

K

I

L

V

Q

D

M

M

T

V

S

N

D

N

-

S

-

T

-

I

-

H

-

R

-

G

-

K

-

V

-

I

-

K

-

E

-

K

-

A

-

K

L

F

G

N

L

W

S

D

D

P

A

E

V

T

Y

V

G

G

F

M

G

I

A
x
H

G

G

I

S

F

F

V

W

G

G

Y

Y

F

I

I

I

F

T

E

Q

I

I

P

P

S

G

N

G

V

Y

I

I

L

A

H

S

K

R

V

L

G

A

A

A

-

N

R

R

V

V

W

F

I

G

A

A

R

A

I

I

M

L

V

L

S

T

W

-

G

-

V

-

I

-

S

S

M

T

L

L

T

N

M

M

F

L

V

I

T

P

T

S

P

A

T

A

M

R

F

V

Y

H

-

Y

-

G

-

C

-

V

-

I

V

F

M

V

R
|
R

F

I

L

L

L

Q

G

G

L

L

A

V

E
|
E

A

G

G

V

F

T

F

Y

P

P

G

A

I

C

I

H

L

G

Y

I

L

W

T

S

Y

K

W

W

Y

A

P

P

A

P

H

L

R

E

R

R

G

S

R

R

M

L

T

A

T

T

W

T

F

S

M

F

T

C

A

G

I

S

A

Y

L

A

S

G

G

A

V

V

I

I

G

A

G

M

P

P

V

L

S

A

G

G

Y

I

I

L

L

V

K

Q

T

-

F

-

N

-

G

-

M

-

N

-

G

-

W

Y

H

T

G

G

W

W

Q

S

W

S

L

V

F

F

L

Y

L

V

E

Y

G

G

I

S

P

F

S

G

V

M

I

V

V

W

G

Y

I

M

M

F

V

W

F

L

T

L

M

V

L

S

D

Y

D

E

R

S

I

P

S

A

K

K

A

H

K

P

W

T

L

I

T

T

K

D

E

E

Q

R

L

Y

L

I

E

E

R

E

H

S

V

I

S

-

A

G

E

E

E

S

A

A

T

N

-

L

-

G

-

A

-

M

-

E

K

K

H

F

D

K

M

T

P

P

I

W

R

R

Q

K

V

F

L

F

T

T

S

S

G

M

R

P

V

V

L

Y

M

A

L

I

S

I

L

V

T

A

Y

N

F

F

S

C

F
x
R

V

S

M

W

G

T

L

F

Y

Y

G

L

V

L

S

L

F

I

W

S

L

Q

P

P

T

A

I

Y

I

F

K

E

A

E

T

V

G

F

V

G

T

F

D

E

A

I

F

S

M

K

I

V

G

G

L

M

L

L

S

S

A

A

I

V

P

P

F

H

A

L

G

V

A

M

V

T

V

I

A

I

M

V

V

P

F

I

V

G

S

G

R

Q

S

I

A

A

D

D

R

F

K

L

R

R

E

S

R

K

-

Q

-

I

-

L

-

S

-

T

-

V

R

R

W

K

H

I

I

M

A

N

L

C

P

G

A

G

F

F

A

G

G

M

A

E

V

A

G

T

L

L

V

L

L

L

S

V

V

V

V

G

W

Y

A

S

H

H

N

T

T

R

V

G

L

V

A

A

M

I

A

S

S

F

L

L

T

V

L

L

A

A

T

-

M

V

G

G

I

F

L

S

T

G

T

F

L

A

P

I

L

S

F

G

W

F

S

N

L

V

P

N

T

H

A

L

I

D

L

I

A

A

G

P

T

R

G

Y

A

A

A

S

A

I

G

L

I

M

A

G

M

I

I

S

N

N

S

G

I

V

G

G

N

T

L

L

A

S

G

G

F

M

L

V

S

C

P

P

Y

I

A

I

V

V

G

G

W

A

L

M

K

T

Q

K

A

N

T

K

A

S

A

R

N

E

D

E

-

W

S

Q

G

Y

M

V

Y

F

M

L

L

I

A

A

F

L

M

V

V

H

L

Y

G

G

L

V

L

I

R88 (= R47) mutation to A: Impairs synaptic transmission. Abolishes the chloride ion conductance.
H128 (≠ A72) mutation to A: Greatly lowers L-glutamate transport.
R184 (= R126) mutation R->A,E,K: Greatly lowers L-glutamate transport.
E191 (= E133) mutation to A: Greatly lowers L-glutamate transport.; mutation E->D,Q: Lowers L-glutamate transport.
R322 (≠ F266) mutation to A: Loss of L-glutamate release. Abolishes the chloride ion conductance.

7t3nA R184q/e191q mutant of rat vesicular glutamate transporter 2 (vglut2)
22% identity, 88% coverage: 42:428/439 of query aligns to 35:434/452 of 7t3nA

query
sites
7t3nA

V

V

A

A

Y

I

L

V

D

D

R

M

V

V

N

N

-

N

-

S

-

T

-

I

-

H

V

R

G

G

F

G

A

K

K

V

L

I

Q

K

M

E

T

K

S

A

D

K

L

F

G

N

L

W

S

D

D

P

A

E

V

T

Y

V

G

G

F

M

G

I

A

H

G

G

I

S

F

F

V

W

G

G

Y
|
Y

F

I

I

I

F

T

E

Q

I

I

P

P

S

G

N

G

V

Y

I

I

L

A

H

S

K

R

V

L

G

A

A

A

-

N

R

R

V

V

W

F

I

G

A

A

R

A

I

I

M

L

V

L

S

T

W

-

G

-

V

-

I

-

S

S

M

T

L

L

T

N

M

M

F

L

V

I

T

P

T

S

P

A

T

A

M

R

F

V

Y

H

-

Y

-

G

-

C

-

V

-

I

V

F

M

V

R

Q

F

I

L

L

L

Q

G

G

L

L

A

V

E

Q

A

G

G

V

F

T

F
x
Y

P

P

G

A

I

C

I

H

L

G

Y

I

L

W

T

S

Y

K

W

W

Y

A

P

P

A

P

H

L

R
x
E

R

R

G

S

R

R

M

L

T

A

T

T

W
x
T

F

S

M

F

T
x
C

A
x
G

I

S

A
x
Y

L
x
A

S

G

G

A

V

V

I
|
I

G

A

G

M

P

P

V

L

S

A

G

G

Y

I

I

L

L

V

K

Q

T

-

F

-

N

-

G

-

M

-

N

-

G

-

W

Y

H

T

G

G

W

W

Q

S

W

S

L

V

F

F

L

Y

L

V

E

Y

G

G

I

S

P

F

S

G

V

M

I

V

V

W

G

Y

I

M

M

F

V

W

F

L

T

L

M

V

L

S

D

Y

D

E

R

S

I

P

S

A

K

K

A

H

K

P

W

T

L

I

T

T

K

D

E
|
E

Q

R

L
x
Y

L

I

E

E

R

E

H

S

V

I

S

-

A

G

E

E

E

S

A

A

T

N

-

L

-

G

-

A

-

M

-

E

K

K

H

F

D

K

M

T

P

P

I

W

R

R

Q

K

V

F

L

F

T

T

S

S

G

M

R

P

V

V

L

Y

M

A

L

I

S

I

L

V

T

A

Y

N

F

F

S

C

F
x
R

V
x
S

M

W

G

T

L
x
F

Y
|
Y

G

L

V

L

S

L

F

I

W

S

L

Q

P

P

T

A

I

Y

I

F

K

E

A

E

T

V

G

F

V

G

T

F

D

E

A

I

F

S

M

K

I

V

G

G

L

M

L

L

S

S

A

A

I

V

P

P

F

H

A

L

G

V

A

M

V

T

V

I

A

I

M

V

V

P

F

I

V

G

S

G

R

Q

S

I

A

A

D

D

R

F

K

L

R

R

E

S

R

K

-

Q

-

I

-

L

-

S

-

T

-

V

R

R

W

K

H

I

I

M

A

N

L

C

P

G

A

G

F

F

A

G

G

M

A

E

V

A

G

T

L

L

V

L

L

L

S

V

V

V

V

G

W

Y

A

S

H

H

N

T

T

R

V

G

L

V

A

A

M

I

A

S

S

F

L

L

T

V

L

L

A

A

T

-

M

V

G

G

I

F

L

S

T

G

T

F

L

A

P

I

L

S

F

G

W

F

S

N

L

V

P

N

T

H

A

L

I

D

L

I

A

A

G

P

T

R

G

Y

A

A

A

S

A

I

G

L

I

M

A

G

M

I

I

S

N
|
N

S

G

I

V

G

G

N

T

L

L

A

S

G

G

F

M

L

V

S

C

P

P

Y

I

A

I

V

V

G

G

W

A

L

M

K

T

Q

K

A

N

T

K

A

S

A

R

N

E

D

E

-

W

S

Q

G

Y

M

V

Y

F

M

L

L

I

A

A

F

L

M

V

V

H

L

Y

G

G

L

V

L

I

binding (1s,3r)-1-aminocyclopentane-1,3-dicarboxylic acid: Y77 (= Y79), Y137 (≠ F137), Y165 (≠ A165), R264 (≠ F266), S265 (≠ V267), F268 (≠ L270), Y269 (= Y271), N392 (= N387)
binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: E152 (≠ R152), T159 (≠ W159), C162 (≠ T162), G163 (≠ A163), A166 (≠ L166), I170 (= I170), E219 (= E226), E220 (= E227), Y223 (≠ L230)

Sites not aligning to the query:

Q51955 4-hydroxybenzoate transporter PcaK from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
21% identity, 87% coverage: 18:400/439 of query aligns to 16:407/448 of Q51955

query
sites
Q51955

S

S

T

F

F

I

E

N

E

Q

A

Q

T

P

Y

L

R

S

K

R

V

Y

S

Q

W

W

R

R

L

V

A

-

P

-

L

-

L

V

M

L

L

L

C

C

Y

F

V

L

V

I

A

V

Y

F

L

L

D
|
D

R

G

V

L

N
x
D

-

T

-

A

V

M

G

G

F

F

A

I

K

A

L

P

Q

A

M

L

T

S

S

Q

D

E

L

W

G

G

L

I

S

D

D

R

A

A

V

S

Y

L

G

G

F

P

G

V

A

M

G

S

I

A

F

A

F

L

V

I

G

G

Y

M

F

V

I

F

F

G

E

A

I

L

P

G

S

S

N
x
G

V

P

I

L

L

A

H
x
D

K

R

V

F

G
|
G

A

R

R

K

V

G

W

V

I

L

A

V

R

G

I

A

M

V

V

L

S

V

W

F

G

G

V

G

I

F

S

S

M

L

L

A

T

S

M

A

F

Y

V

A

T

T

T

N

P

V

T

D

M

Q

F

L

Y

L

V

V

M

L

R
|
R

F

F

L

L

L

T

G

G

L

L

A

G

E

L

A

G

G

A

F

G

F

M

P

P

G

N

I

A

I

T

L

T

Y

L

L

L

T

S

Y
x
E

W

Y

Y

T

P

P

A

E

H

R

R

L

R

K

G

S

R

L

M

L

T

V

T

T

W

S

F

M

M

F

T

C

A

G

I

F

A

N

L

L

S

G

G

M

V

A

I

G

G

G

P

F

V

I

S

S

G

A

Y

K

I

M

L

I

K

P

T

A

F

Y

N

-

G

-

M

-

N

-

G

-

W

-

H

-

G

G

W

W

Q
x
H

W

S

L

L

F

L

L

V

L

I

E

G

G

G

-

V

-

L

-

P

-

L

-

A

-

L

-

V

-

L

-

M

-

V

-

W

I

L

P

P

S

E

V

S

I

A

V

R

G

F

I

L

M

V

V

V

F

R

T

N

M

R

L

G

D

T

D

D

R

K

I

I

S

R

K

K

A

T

K

-

W

-

L

L

T

S

K

P

E

I

E

A

Q

P

Q

Q

L

V

E

A

R

E

H

A

V

G

S

S

A

F

E

S

E

V

A

P

T

E

K

Q

H

K

D

A

M

V

P

A

I

A

R

R

Q

S

V

V

L

F

T

A

-

V

-

I

-

F

S

S

G

G

R

T

-

Y

-

G

-

L

-

G

V

T

L

M

M

L

L

L

S

W

L

L

T

T

Y

Y

F

F

S

M

F

G

V

L

M

V

G

I

L

V

Y

Y

G

L

V

L

S

T

F

S

W

W

L

L

P

P

T

T

I

L

I

M

K

R

A

D

T

S

G

G

V

A

T

S

-

M

-

E

D

Q

A

A

F

A

M

F

I

I

G

G

L

A

L

L

S

F

A

Q

I

F

P

-

F

-

A

-

G

G

A

G

V

V

V

L

A

S

M

A

V

V

F

G

V

V

S

G

R

W

S

A

A

M

D
|
D

R

R

K

Y

R

N

E

P

R
x
H

R

K

-

V

-

I

-

G

-

I

W

F

H

Y

I

L

A

L

L

A

P

G

A

V

F

F

A

A

G

Y

A

A

V

V

G

G

L

Q

V

S

L

L

S

G

V

N

V

I

W

T

A

V

H

L

N

A

T

T

V

L

L

V

A

L

M

I

A

A

S

G

L

M

T

C

L

V

A

N

T

-

M

-

G

G

I

A

L

Q

T

S

T

A

L

M

P

P

L

-

F

-

W

-

S

S

L

L

P

A

T

A

A

R

I

F

L

Y

A

P

G

T

T

Q

G

G

A
x
R

A

A

A

T

G

G

I

V

A

S

M

W

I

M

N

L

S

G

I

I

G

G

N
x
R

L

F

A

G

G

A

F

I

L

L

S

G

P

A

Y

W

A

S

D41 (= D46) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
D44 (≠ N49) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
G85 (≠ N87) mutation to V: Abolishes 4-HBA transport and chemotaxis.
D89 (≠ H91) mutation to N: Abolishes 4-HBA transport and chemotaxis.
G92 (= G94) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to C: No change in 4-HBA transport and chemotaxis.; mutation G->L,V: Abolishes 4-HBA transport and chemotaxis.; mutation to Q: Decrease in 4-HBA transport and strong decrease in chemotaxis.
R124 (= R126) mutation to A: Abolishes 4-HBA transport.
E144 (≠ Y146) mutation to A: Strong decrease in 4-HBA transport.
H183 (≠ Q192) mutation to A: Decrease in 4-HBA transport and chemotaxis.
D323 (= D315) mutation to N: Abolishes 4-HBA transport and chemotaxis.
H328 (≠ R320) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to R: Decrease in 4-HBA transport and loss of chemotaxis.
R386 (≠ A379) mutation to A: Strong decrease in 4-HBA transport.
R398 (≠ N391) mutation to A: Abolishes 4-HBA transport.

Sites not aligning to the query:

444 H→A: No change in 4-HBA transport and chemotaxis.

Q9C0U9 Uncharacterized transporter PB1C11.03 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
23% identity, 46% coverage: 27:228/439 of query aligns to 93:296/570 of Q9C0U9

query
sites
Q9C0U9

K

K

V

L

S

K

W

W

R

K

L

L

A

Y

P

I

L

Y

L

L

M

L

L

L

C

-

Y

-

V

M

V

L

A

G

Y

F

L

L

D

D

R

M

-

M

V

L

N

F

V

I

G

G

F

K

A

A

K

T

L

L

Q

S

M

Y

T

S

S

T

D

I

L

L

G

G

L

L

S

F

D

D

A

D

V

V

-

H

-

I

-

T

-

S

-

N

-

Q

Y

Y

G

N

F

N

G

L

A

N

G

T

I

L

F

F

F

Y

V

V

G

G

Y

Y

F

I

I

V

F

G

E

Q

I

F

P

P

S

G

N

H

V

Y

I

I

L

M

H

Q

K

T

V

F

G

P

A

L

R

G

V

K

W

F

I

V

A

G

R

L

I

V

M

T

V

F

S

S

W

W

G

S

V

V

I

I

S

V

M

F

L

L

T

H

M

C

F

C

V

A

T

Y

T

N

P

Y

T

G

M

G

F

L

Y

I

V

A

M

L

R

R

F

F

L

F

L

L

G

G

L

F

A

T

E

E

A

S

G

C

F

L

P

P

G

A

I

M

I

E

L

A

Y

T

L

M

T

G

Y

M

W

F

Y

F

P

T

A

H

H

Q

R

E

R

Q

G

A

R

F

M

L

T

Q

T

P

W

V

F

F

M

W

T

I

A

S

I

C

A

L

L

S

S

C

G

G

V

I

I

P

G

A

G

G

P

F

V

I

S

A

-

Y

-

G

-

L

G

E

Y

F

I

V

L

T

K

K

T

S

F

I

N

-

G

-

M

-

N

-

G

-

W

-

H

A

G

P

W

W

Q

K

W

L

L

F

F

M

L

I

E

T

G

G

I

G

P

I

S

T

V

F

I

F

V

L

G

S

I

I

M

F

V

L

F

F

T

F

M

Y

L

Y

D

P

D

D

R

N

I

P

S

S

K

K

A

A

K

R

W

F

L

L

T

T

K

D

E

E

Q

K

Sites not aligning to the query:

14 modified: Phosphoserine

Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see paper)
24% identity, 65% coverage: 31:316/439 of query aligns to 40:313/444 of Q8NLB7

query
sites
Q8NLB7

R

R

L

L

A

A

P

A

L

V

L

L

M

I

L

G

C

W

Y

F

V

F

V

V

A

I

Y

F

-
x
D

-

G

L

Y

D
|
D

R

L

V

I

N

V

V

Y

G

G

F

T

A

V

K

Q

L

S

Q

A

M

L

T

A

S

K

D

E

L

W

G

N

L

L

S

S

D

S

A

A

V

T

Y

L

G

G

F

T

G

I

A

G

G

S

I

T

F

A

F

F

V

F

G

G

Y

M

F

A

I

I

F

G

E

A

I

V

P

F

S

I

N

G

V

R

I

L

L

S

H

D

K
x
R

V

V

G

G

A

R

R

K

V

A

W

A

I

V

A

I

R

G

I

S

M

V

V

L

S

I

W

L

G

S

V

V

I

F

S

T

M

M

L

L

T

C

M

A

F

F

V

A

T

P

T

N

P

P

T

W

M

V

F

F

Y

G

V

A

M

F

R

R

F

F

L

I

L

A

G

G

L

L

A

G

E

L

A

G

G

G

F

L

F

V

P

P

G

S

I

V

I

N

L

A

Y

M

L

T

T

S

Y

D

W

L

Y

V

P

P

A

-

H

-

R

-

R

R

G

K

R

T

M

M

T

S

T

A

W

W

F

-

M

-

T

A

A

T

I

V

A

M

L

M

S

S

G

G

V

V

-

P

I

I

G

G

P

S

V

I

S

A

G

A

Y

V

I

L

L

A

K

L

T

V

F

V

N

V

G

P

M

S

N

S

G

E

W

E

H

W

G

G

W

W

Q

R

W

F

L

M

F

F

L

L

L

I

E

A

G

L

I

I

P

P

S

L

V

V

I

V

-

G

V

L

G

P

I

I

M

A

V

M

F

K

T

V

M

I

L

P

D

S

D

D

R

K

I

A

S

I

K

K

A

A

K

-

W

-

L

-

T

-

K

-

E

-

E

D

Q

H

Q

D

L

I

L

R

E

E

R

G

H

H

V

-

S

-

A

D

E

E

E

P

A

A

T

G

K

F

H

K

D

D

M

L

P

L

I

V

R

D

Q

R

V

Y

L

R

T

W

S

I

G

S

R

-

V

-

L

I

M

W

L

F

S

A

L

L

T

A

Y

T

F

F

S

V

F

T

V

L

M

L

G

A

L

W

Y

Y

G

G

V

L

S

G

F

T

W

W

L

L

P

P

T

R

I

L

I

M

K

E

A

T

T

A

G

G

V

Y

T

E

D

F

A

G

F

H

M

A

I

L

G

M

L

F

L

T

S

L

A

A

I

L

P

N

F

L

A

-

G

G

A

A

V

V

V

I

A

G

M

S

V

V

F

V

V

T

S

A

R
x
W

S

A

A

G

D
|
D

R
|
R

D54 (vs. gap) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
D57 (= D46) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
R103 (≠ K92) mutation to A: Loss of transport activity.
W309 (≠ R312) mutation to V: Loss of transport activity.
D312 (= D315) mutation to A: Loss of transport activity.
R313 (= R316) mutation to A: Loss of transport activity.

Sites not aligning to the query:

317 mutation I->H,Y: Loss of transport activity.
386 R→A: Loss of transport activity.

7aarA Sugar/h+ symporter stp10 in inward open conformation (see paper)
22% identity, 54% coverage: 86:321/439 of query aligns to 87:334/485 of 7aarA

query
sites
7aarA

S

A

N

S

V

V

I
|
I

L

T

H

R

K
|
K

V
x
H

G

G

A

R

R
x
K

V
|
V

W

S

I

M

A
x
F

R

I

I

G

M

G

V

L

S

A

W

F

G

L

V

I

I

G

S

A

M

L

L

F

T

N

M

A

F

F

V

A

T

V

T

N

P

V

T

S

M

M

F

L

Y

I

V

I

M

G

R

R

F

L

L

L

G

G

L

V

A

G

E
x
V

A

G

G

F

F

A

F
x
N

P

Q

G

S

I

T

I
x
P

L

V

Y

Y

L

L

T

S

Y

Q

W

M

Y

A

P

P

A

A

H

K

R

I

R

R

G

G

R

A

M

L

T
x
N

T

I

W

G

F
|
F

M
x
Q

T

M

A

A

I
|
I

A

T

L

I

S

-

G

-

V

-

I

-

G

-

G

G

P

I

V

L

S

V

G

A

Y

N

I

L

L

I

K

N

T

Y

F

G

N

T

G

S

M

K

N

M

G

A

W

Q

H

H

G

G

W

W

Q

R

W

V

L

S

F

L

L

G

L

L

E

A

G

A

I

V

P

P

S

A

V

V

I

V

V

M

G

V

I

I

M

G

V

S

F
|
F

T

I

M

L

L

P

D
|
D

D

T

R

P

I

N

S

S

K

M

A

L

K

E

W
x
R

L

G

T

K

K

N

E

E

-

A

Q

K

Q

Q

L

M

L

L

E

K

R

K

H

I

V

R

S

G

A

A

-

D

-

N

-

V

-

D

-

H

-

E

-

F

-

Q

-

D

-

L

-

I

-

D

-

A

-

V

E

E

E

A

A

A

T

K

K

K

H

V

D

E

M

N

P

P

I

W

R

K

Q

N

V

I

L

M

T

E

S

S

-

K

-

Y

-

R

-

P

G

A

R

L

V

I

L

F

M

C

L

S

S

A

L

I

T

P

Y

F

F

F

S
x
Q

F
x
Q

V

I

M

T