SitesBLAST
Comparing BPHYT_RS05425 FitnessBrowser__BFirm:BPHYT_RS05425 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1cqxA Crystal structure of the flavohemoglobin from alcaligenes eutrophus at 1.75 a resolution (see paper)
42% identity, 35% coverage: 343:587/700 of query aligns to 154:398/403 of 1cqxA
- binding flavin-adenine dinucleotide: Y190 (= Y379), R206 (= R395), Q207 (≠ T396), Y208 (= Y397), S209 (≠ T398), S222 (= S411), E226 (= E415), Q231 (vs. gap), P232 (vs. gap), G234 (= G418), Y235 (≠ G419), V236 (≠ C420), S237 (= S421), V395 (≠ S584), G397 (= G586), P398 (= P587)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 46, 47
- binding protoporphyrin ix containing fe: 43, 44, 81, 85, 88, 90, 94, 95, 98, 126, 129, 130, 133
3ozwA The crystal structure of flavohemoglobin from r. Eutrophus in complex with ketoconazole (see paper)
41% identity, 35% coverage: 343:587/700 of query aligns to 154:398/403 of 3ozwA
- binding flavin-adenine dinucleotide: R206 (= R395), Q207 (≠ T396), Y208 (= Y397), S209 (≠ T398), S222 (= S411), V223 (= V412), K224 (= K413), E226 (= E415), P232 (vs. gap), G234 (= G418), Y235 (≠ G419), V236 (≠ C420), S237 (= S421), V276 (= V460), T279 (= T463), V395 (≠ S584), F396 (= F585)
- binding 1-acetyl-4-(4-{[(2R,4S)-2-(2,4-dichlorophenyl)-2-(1H-imidazol-1-ylmethyl)-1,3-dioxolan-4-yl]methoxy}phenyl)piperazine: P398 (= P587)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 46, 50
- binding protoporphyrin ix containing fe: 42, 43, 81, 85, 88, 90, 94, 95, 98, 126, 130, 133
- binding 1-acetyl-4-(4-{[(2R,4S)-2-(2,4-dichlorophenyl)-2-(1H-imidazol-1-ylmethyl)-1,3-dioxolan-4-yl]methoxy}phenyl)piperazine: 53, 56, 102
3ozvA The crystal structure of flavohemoglobin from r. Eutrophus in complex with econazole (see paper)
41% identity, 35% coverage: 343:587/700 of query aligns to 154:398/403 of 3ozvA
- binding flavin-adenine dinucleotide: R206 (= R395), Q207 (≠ T396), Y208 (= Y397), S209 (≠ T398), S222 (= S411), V223 (= V412), K224 (= K413), E226 (= E415), P232 (vs. gap), G234 (= G418), Y235 (≠ G419), V236 (≠ C420), S237 (= S421), V276 (= V460), T279 (= T463), F396 (= F585)
Sites not aligning to the query:
- binding 1-[(2s)-2-[(4-chlorobenzyl)oxy]-2-(2,4-dichlorophenyl)ethyl]-1h-imidazole: 24, 28, 57, 102, 106
- binding flavin-adenine dinucleotide: 44, 46, 48
- binding protoporphyrin ix containing fe: 43, 81, 84, 85, 88, 90, 94, 95, 98, 126, 130, 133
3ozuA The crystal structure of flavohemoglobin from r. Eutrophus in complex with miconazole (see paper)
41% identity, 35% coverage: 343:587/700 of query aligns to 154:398/403 of 3ozuA
- binding flavin-adenine dinucleotide: R206 (= R395), Q207 (≠ T396), Y208 (= Y397), S209 (≠ T398), S222 (= S411), V223 (= V412), K224 (= K413), E226 (= E415), P232 (vs. gap), G234 (= G418), Y235 (≠ G419), V236 (≠ C420), S237 (= S421), E394 (= E583), V395 (≠ S584), G397 (= G586), P398 (= P587)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 46, 47
- binding protoporphyrin ix containing fe: 43, 44, 81, 85, 88, 90, 94, 95, 98, 126, 129, 130, 133
- binding 1-[(2R)-2-[(2,4-dichlorobenzyl)oxy]-2-(2,4-dichlorophenyl)ethyl]-1H-imidazole: 25, 28, 43, 56, 57, 102, 122, 125, 126
P39662 Flavohemoprotein; FHP; Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD; EC 1.14.12.17 from Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) (Ralstonia eutropha) (see paper)
41% identity, 35% coverage: 343:587/700 of query aligns to 154:398/403 of P39662
Sites not aligning to the query:
- 60 A→Y: Does not affect phospholipid-binding.
- 98 V→F: Blocks phospholipid-binding.
1gvhA The x-ray structure of ferric escherichia coli flavohemoglobin reveals an unespected geometry of the distal heme pocket (see paper)
40% identity, 35% coverage: 344:590/700 of query aligns to 153:395/396 of 1gvhA
- binding flavin-adenine dinucleotide: Y188 (= Y379), R204 (= R395), Q205 (≠ T396), Y206 (= Y397), S207 (≠ T398), A220 (≠ S411), V221 (= V412), E224 (= E415), G227 (= G418), Q228 (≠ G419), V229 (≠ C420), S230 (= S421), V269 (= V460), T272 (= T463), E388 (= E583), F390 (= F585)
- binding protoporphyrin ix containing fe: H393 (≠ A588)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 46, 48, 50
- binding protoporphyrin ix containing fe: 43, 53, 56, 57, 60, 61, 81, 84, 85, 90, 94, 95, 127, 131
P24232 Flavohemoprotein; Flavohemoglobin; HMP; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD; EC 1.14.12.17 from Escherichia coli (strain K12) (see 2 papers)
40% identity, 35% coverage: 344:590/700 of query aligns to 153:395/396 of P24232
Sites not aligning to the query:
- 29 mutation Y->E,H: 15 to 35-fold reduction in NO dioxygenase activity.; Y→F: 30-fold reduction in NO dioxygenase activity, and 80-fold increase in the O(2) dissociation rate constant.
- 95 active site, Charge relay system
- 135 active site, Charge relay system
4eh1A Crystal structure of the flavohem-like-fad/NAD binding domain of nitric oxide dioxygenase from vibrio cholerae o1 biovar el tor
42% identity, 35% coverage: 344:587/700 of query aligns to 4:237/237 of 4eh1A
- binding flavin-adenine dinucleotide: Y39 (= Y379), R55 (= R395), Q56 (≠ T396), Y57 (= Y397), S58 (≠ T398), S71 (= S411), V72 (= V412), E75 (= E415), N81 (vs. gap), G83 (vs. gap), L84 (vs. gap), V85 (≠ C420), S86 (= S421), T127 (= T463), E233 (= E583), F235 (= F585)
4g1bA X-ray structure of yeast flavohemoglobin in complex with econazole (see paper)
32% identity, 37% coverage: 330:587/700 of query aligns to 136:392/398 of 4g1bA
- binding flavin-adenine dinucleotide: Y189 (= Y379), R207 (= R395), H208 (≠ T396), Y209 (= Y397), S210 (≠ T398), A223 (≠ S411), K225 (= K413), E227 (= E415), F233 (vs. gap), P234 (vs. gap), G236 (= G418), L237 (≠ G419), V238 (≠ C420), S239 (= S421), V282 (= V460), F390 (= F585)
- binding protoporphyrin ix containing fe: P392 (= P587)
Sites not aligning to the query:
- binding 1-[(2s)-2-[(4-chlorobenzyl)oxy]-2-(2,4-dichlorophenyl)ethyl]-1h-imidazole: 28, 29, 56, 57, 60, 102, 126
- binding flavin-adenine dinucleotide: 46, 50, 84
- binding protoporphyrin ix containing fe: 42, 43, 44, 47, 60, 80, 81, 84, 85, 88, 90, 94, 95, 98, 133, 393, 394
2piaA Phthalate dioxygenase reductase: a modular structure for electron transfer from pyridine nucleotides to [2fe-2s] (see paper)
27% identity, 46% coverage: 358:679/700 of query aligns to 23:311/321 of 2piaA
- binding fe2/s2 (inorganic) cluster: S270 (≠ Y639), C272 (= C641), E273 (≠ R642), G275 (= G644), C277 (= C646), G278 (= G647), C280 (= C649), C308 (= C676)
- binding flavin mononucleotide: N44 (≠ Y379), R55 (= R395), T56 (= T396), Y57 (= Y397), S58 (≠ T398), A72 (≠ S411), V73 (= V412), G79 (= G416), R80 (≠ K417), G82 (= G419), S83 (≠ C420), I121 (≠ V460), T124 (= T463), E223 (= E583), F225 (= F585)
P33164 Phthalate dioxygenase reductase; PDR; EC 1.-.-.- from Burkholderia cepacia (Pseudomonas cepacia) (see paper)
27% identity, 46% coverage: 358:679/700 of query aligns to 24:312/322 of P33164
Q03331 Flavohemoprotein; Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD; EC 1.14.12.17 from Candida norvegensis (Yeast) (Candida mycoderma) (see paper)
28% identity, 37% coverage: 330:588/700 of query aligns to 151:386/390 of Q03331
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylserine
7ylrA Structure of a bacteria protein
26% identity, 48% coverage: 346:679/700 of query aligns to 8:317/326 of 7ylrA
- binding fe2/s2 (inorganic) cluster: C276 (= C641), K277 (≠ R642), G279 (= G644), C281 (= C646), G282 (= G647), C284 (= C649), C314 (= C676)
- binding flavin mononucleotide: R56 (≠ A390), H57 (≠ E391), Y58 (≠ P392), S59 (≠ T393), A79 (≠ S411), V80 (= V412), R81 (≠ K413), G86 (= G418), R87 (vs. gap), G89 (≠ C420), S90 (= S421), T131 (= T463), E229 (= E583), F231 (= F585), E233 (= E595), R278 (≠ S643)
6laaA Crystal structure of full-length cyp116b46 from tepidiphilus thermophilus (see paper)
27% identity, 46% coverage: 338:662/700 of query aligns to 434:723/753 of 6laaA
- binding fe2/s2 (inorganic) cluster: S700 (≠ Y639), C702 (= C641), E703 (≠ R642), G705 (= G644), C707 (= C646), G708 (= G647), C710 (= C649)
- binding flavin mononucleotide: R487 (= R395), Q488 (≠ T396), Y489 (= Y397), S490 (≠ T398), Q506 (≠ K413), S511 (≠ K417), R512 (≠ G418), G514 (≠ C420), S515 (= S421), I553 (≠ V460), E651 (vs. gap), F653 (vs. gap)
Sites not aligning to the query:
- active site: 177, 251, 252, 359, 360, 361
- binding carbonate ion: 90, 91, 92, 241
- binding fe2/s2 (inorganic) cluster: 740
- binding protoporphyrin ix containing fe: 54, 91, 92, 99, 103, 249, 252, 253, 298, 351, 352, 353, 357, 359, 361
6kbhA Crystal structure of an intact type iv self-sufficient cytochrome p450 monooxygenase
28% identity, 38% coverage: 395:660/700 of query aligns to 499:733/765 of 6kbhA
- binding fe2/s2 (inorganic) cluster: C714 (= C641), E715 (≠ R642), G717 (= G644), C719 (= C646), G720 (= G647), C722 (= C649)
- binding flavin mononucleotide: R499 (= R395), Q500 (≠ T396), Y501 (= Y397), S502 (≠ T398), A516 (≠ S411), L518 (vs. gap), S523 (≠ G416), R524 (≠ K417), G526 (= G419), S527 (= S421), I565 (≠ V460), T568 (= T463), E663 (= E583), F665 (= F585), I718 (≠ S645)
Sites not aligning to the query:
- active site: 190, 264, 265, 372, 373, 374
- binding fe2/s2 (inorganic) cluster: 752
- binding protoporphyrin ix containing fe: 67, 104, 105, 112, 116, 261, 262, 265, 266, 308, 311, 313, 364, 365, 370, 372, 373, 374, 378
6o0aA Crystal structure of flavohemoglobin from malassezia yamatoensis with bound fad and heme determined by iron sad phasing (see paper)
24% identity, 36% coverage: 332:586/700 of query aligns to 142:383/383 of 6o0aA
- binding flavin-adenine dinucleotide: Y190 (= Y379), R205 (= R395), Q206 (≠ T396), F207 (≠ Y397), T208 (= T398), K222 (= K413), D224 (≠ E415), H226 (≠ K417), G227 (= G418), E228 (≠ G419), M229 (≠ C420), T230 (≠ S421), T274 (= T463), E380 (= E583), F382 (= F585), G383 (= G586)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 48, 51, 52
- binding protoporphyrin ix containing fe: 44, 45, 46, 59, 62, 84, 85, 89, 92, 94, 98, 99, 102, 130, 133
5ogxA Crystal structure of amycolatopsis cytochrome p450 reductase gcob. (see paper)
26% identity, 35% coverage: 348:589/700 of query aligns to 104:333/333 of 5ogxA
- binding flavin-adenine dinucleotide: Y132 (= Y379), R144 (= R395), Q145 (≠ T396), Y146 (= Y397), S147 (≠ T398), H161 (≠ S411), V162 (= V412), V165 (≠ E415), G168 (= G418), V169 (≠ G419), A170 (≠ C420), T171 (≠ S421), T214 (≠ V460), F329 (= F585), P331 (= P587), S333 (= S589)
Sites not aligning to the query:
P0DPQ8 Aromatic O-demethylase, reductase subunit; NADH--hemoprotein reductase; EC 1.6.2.- from Amycolatopsis sp. (strain ATCC 39116 / 75iv2) (see paper)
26% identity, 35% coverage: 348:589/700 of query aligns to 105:334/334 of P0DPQ8
Sites not aligning to the query:
- 35 binding
- 40 binding
- 43 binding
- 75 binding
7romA Crystal structure of saccharomyces cerevisiae nadh-cytochrome b5 reductase 1 (cbr1) fragment (residues 28-284) bound to fad
23% identity, 34% coverage: 340:579/700 of query aligns to 8:243/255 of 7romA
- binding flavin-adenine dinucleotide: R61 (= R395), S62 (≠ T396), Y63 (= Y397), T64 (= T398), L79 (≠ S411), V80 (= V412), Y83 (≠ E415), T85 (≠ K417), G86 (= G418), N87 (≠ G419), V88 (≠ C420), S89 (= S421), T127 (≠ V460), A130 (≠ T463)
7qu0A X-ray structure of fad domain of nqrf of klebsiella pneumoniae (see paper)
25% identity, 28% coverage: 391:585/700 of query aligns to 79:279/279 of 7qu0A
- binding ~{N}-[2,6-bis(fluoranyl)phenyl]ethanamide: S213 (= S520), F227 (≠ Q533), M256 (≠ F562)
- binding flavin-adenine dinucleotide: R83 (= R395), A84 (≠ T396), Y85 (= Y397), S86 (≠ T398), A104 (vs. gap), T105 (= T407), P106 (≠ Y408), P107 (≠ R409), A113 (≠ E415), P114 (≠ G416), P115 (≠ K417), G116 (= G418), I117 (≠ G419), M118 (≠ C420), S119 (= S421), F279 (= F585)
Sites not aligning to the query:
Query Sequence
>BPHYT_RS05425 FitnessBrowser__BFirm:BPHYT_RS05425
MSSSTVPSSPGWDLERSPFHEGELAVQERVGVREKIDAQGRRAVRRYLTAQQREFFLMLP
YVFMASVDSSGRPWASFLVGEPGFVNPTGDVTLTVRARALYGDPLNDTLVPGAEIALLGV
QFHTRRRNRVIGTVGALLPDGFTIDVRQTLGICPQYIQGRELSFNRDPLLPEPRPVHRNS
RLDASASAIISKADTYFVASVLLQEGDPVATGADVSHRGGRPGFVRIDDDHTLTAPDFVG
NFIFNTIGNWQFDNHAGLMFIDFDSGAMLYIAAHAEVLWDGPELHAFNGAQRLFRYHIDE
VCRVEASLPCTFTAPDYSPLLERTGHWDIVAKTLEAERLRTVWRPFKVLETIDESATIRS
FILEPADGSGLVGYQAGQYLPIRLQPPGWAEPTIRTYTLSDASNGRTYRISVKREGKGGC
SDFLHDHIVVGDTIETLAPRGAFVFDEAAGRPLALISAGVGITPLIAMLNSQLVNDGRTL
LHKPIYCIHSATNSKHRAFAAHLAKKGELHANLTLHVAFSRPSEDDVLGKTHQSEGYIDR
ALLRRVLPLDDYEVYLCGPPGFMQSVYDALLSLGVRDSRIHLESFGPASVSRRIERTVEV
DNSEGVVVTFAKSGRNAIWRPKVGSLLELAEANGLKPLYACRSGSCGTCVTRVVKGEVDY
TEPPAHDVEPGEALICIARPHPGPHLEDGTQNREGVTLDI
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory