SitesBLAST
Comparing BPHYT_RS06125 FitnessBrowser__BFirm:BPHYT_RS06125 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P33221 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; GAR transformylase T; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Escherichia coli (strain K12) (see 5 papers)
62% identity, 97% coverage: 7:399/404 of query aligns to 4:389/392 of P33221
- EL 22:23 (= EL 25:26) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- E82 (= E85) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- R114 (= R118) binding ATP
- K155 (= K159) binding ATP
- SSGKGQ 160:165 (= SSGKGQ 164:169) binding ATP
- G162 (= G166) mutation to I: Strong decrease in the reaction rate for the conversion of formate to FGAR and in the affinity for formate. 3- and 2-fold decrease in the affinity for ATP and GAR, respectively.
- K179 (≠ Q183) modified: N6-acetyllysine
- EGVV 195:198 (≠ EGFI 199:202) binding ATP
- E203 (= E207) binding ATP
- E267 (= E277) binding Mg(2+)
- E279 (= E289) binding Mg(2+)
- D286 (= D296) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- K355 (= K365) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- RR 362:363 (= RR 372:373) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
1kjiA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-amppcp (see paper)
62% identity, 97% coverage: 7:399/404 of query aligns to 3:386/389 of 1kjiA
- active site: E114 (= E119), K154 (= K159), S159 (= S164), G161 (= G166), E264 (= E277), E276 (= E289), D283 (= D296), T284 (= T297), R360 (= R373)
- binding phosphomethylphosphonic acid adenylate ester: R113 (= R118), I152 (≠ V157), K154 (= K159), S159 (= S164), S160 (= S165), G161 (= G166), Q164 (= Q169), E192 (= E199), V195 (≠ I202), E200 (= E207), Q222 (= Q235), E264 (= E277), F266 (= F279), E276 (= E289)
- binding magnesium ion: E264 (= E277), E276 (= E289)
1ez1A Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg, amppnp, and gar (see paper)
62% identity, 97% coverage: 7:399/404 of query aligns to 3:386/389 of 1ez1A
- active site: E114 (= E119), K154 (= K159), S159 (= S164), G161 (= G166), E264 (= E277), E276 (= E289), D283 (= D296), T284 (= T297), R360 (= R373)
- binding phosphoaminophosphonic acid-adenylate ester: R113 (= R118), I152 (≠ V157), K154 (= K159), S159 (= S164), S160 (= S165), G161 (= G166), E192 (= E199), V194 (≠ F201), V195 (≠ I202), F197 (= F204), E200 (= E207), Q222 (= Q235), E264 (= E277), F266 (= F279), E276 (= E289)
- binding glycinamide ribonucleotide: G20 (= G24), E21 (= E25), L22 (= L26), E81 (= E85), I82 (= I86), S160 (= S165), D283 (= D296), K352 (= K365), R359 (= R372), R360 (= R373)
- binding magnesium ion: E264 (= E277), E276 (= E289)
1eyzA Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg and amppnp (see paper)
62% identity, 97% coverage: 7:399/404 of query aligns to 3:386/389 of 1eyzA
- active site: E114 (= E119), K154 (= K159), S159 (= S164), G161 (= G166), E264 (= E277), E276 (= E289), D283 (= D296), T284 (= T297), R360 (= R373)
- binding phosphoaminophosphonic acid-adenylate ester: R113 (= R118), I152 (≠ V157), K154 (= K159), S159 (= S164), S160 (= S165), G161 (= G166), Q164 (= Q169), E192 (= E199), V195 (≠ I202), F197 (= F204), E200 (= E207), E264 (= E277), F266 (= F279), E276 (= E289)
- binding magnesium ion: E264 (= E277), E276 (= E289)
1kjjA Crystal structure of glycniamide ribonucleotide transformylase in complex with mg-atp-gamma-s (see paper)
62% identity, 97% coverage: 7:399/404 of query aligns to 3:383/386 of 1kjjA
- active site: E114 (= E119), K154 (= K159), S159 (= S164), G161 (= G166), E261 (= E277), E273 (= E289), D280 (= D296), T281 (= T297), R357 (= R373)
- binding phosphothiophosphoric acid-adenylate ester: R113 (= R118), I152 (≠ V157), K154 (= K159), S159 (= S164), S160 (= S165), G161 (= G166), Q164 (= Q169), E189 (= E199), V192 (≠ I202), E197 (= E207), Q219 (= Q235), E261 (= E277), F263 (= F279), E273 (= E289)
- binding magnesium ion: E261 (= E277), E273 (= E289)
1kj8A Crystal structure of purt-encoded glycinamide ribonucleotide transformylase in complex with mg-atp and gar (see paper)
62% identity, 97% coverage: 7:399/404 of query aligns to 3:383/386 of 1kj8A
- active site: E114 (= E119), K154 (= K159), S159 (= S164), G161 (= G166), E261 (= E277), E273 (= E289), D280 (= D296), T281 (= T297), R357 (= R373)
- binding adenosine-5'-triphosphate: R113 (= R118), I152 (≠ V157), K154 (= K159), S159 (= S164), S160 (= S165), G161 (= G166), Q164 (= Q169), E189 (= E199), V192 (≠ I202), F194 (= F204), E197 (= E207), Q219 (= Q235), G222 (= G238), E261 (= E277), F263 (= F279), E273 (= E289)
- binding glycinamide ribonucleotide: G20 (= G24), E21 (= E25), L22 (= L26), E81 (= E85), I82 (= I86), S160 (= S165), D280 (= D296), K349 (= K365), R356 (= R372)
- binding magnesium ion: E261 (= E277), E273 (= E289)
1kjqA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-adp (see paper)
61% identity, 97% coverage: 7:399/404 of query aligns to 3:385/388 of 1kjqA
- active site: E114 (= E119), K154 (= K159), E263 (= E277), E275 (= E289), D282 (= D296), T283 (= T297), R359 (= R373)
- binding adenosine-5'-diphosphate: R113 (= R118), I152 (≠ V157), K154 (= K159), E191 (= E199), V193 (≠ F201), V194 (≠ I202), F196 (= F204), E199 (= E207), Q221 (= Q235), F265 (= F279), E275 (= E289)
- binding magnesium ion: E263 (= E277), E275 (= E289)
O58056 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
47% identity, 99% coverage: 1:398/404 of query aligns to 2:408/430 of O58056
2dwcB Crystal structure of probable phosphoribosylglycinamide formyl transferase from pyrococcus horikoshii ot3 complexed with adp
45% identity, 99% coverage: 1:398/404 of query aligns to 4:397/409 of 2dwcB
- active site: E265 (= E277), E277 (= E289), D284 (= D296), T285 (= T297), R372 (= R373)
- binding adenosine-5'-diphosphate: R120 (= R118), H159 (≠ V157), K161 (= K159), H190 (≠ F201), I191 (= I202), F193 (= F204), E196 (= E207), F267 (= F279), E277 (= E289)
3v4sA Crystal structure of adp-atp complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
29% identity, 93% coverage: 16:392/404 of query aligns to 1:366/380 of 3v4sA
- binding adenosine-5'-diphosphate: R106 (= R118), K146 (= K159), Y152 (vs. gap), G154 (= G166), Q157 (= Q169), W183 (≠ F201), V184 (≠ I202), E189 (= E207), N215 (≠ G238), F256 (= F279), N266 (≠ S288), E267 (= E289)
- binding carbonate ion: R271 (= R293), H273 (= H295), N274 (≠ D296)
3v4sB Crystal structure of adp-atp complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
29% identity, 93% coverage: 16:392/404 of query aligns to 3:368/381 of 3v4sB