SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS07505 FitnessBrowser__BFirm:BPHYT_RS07505 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see paper)
26% identity, 47% coverage: 4:216/453 of query aligns to 3:206/444 of Q8NLB7

query
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Q8NLB7

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D54 (= D48) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
D57 (≠ I51) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
R103 (= R100) mutation to A: Loss of transport activity.

Sites not aligning to the query:

309 W→V: Loss of transport activity.
312 D→A: Loss of transport activity.
313 R→A: Loss of transport activity.
317 mutation I->H,Y: Loss of transport activity.
386 R→A: Loss of transport activity.

Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
24% identity, 85% coverage: 12:395/453 of query aligns to 2:382/452 of Q5EXK5

query
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Q5EXK5

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D82 (= D99) mutation to A: Loss of activity.
V311 (≠ A321) mutation to W: Loss of activity.
D314 (= D324) mutation to A: Loss of activity.

Query Sequence

>BPHYT_RS07505 FitnessBrowser__BFirm:BPHYT_RS07505
MDYSATPQPAGTEALSMQSTPAAQPVERTSHVKAIAAITLGNGLEFFDFTIYSFFATIIG
KLYFPVEGQLAQLMLAVGTFGVGFIMRPVGGIVLGAYADRAGRKAAMSLTLWLMTLGSAI
IAFAPTYAAIGVAAPLLVILARLIQGFALGGEVGASTSLLLEYGSNKTRGFYGSWQFVSQ
GLNTVCGSLLGVALAAALSTAALESWGWRVPFVIGMAMGPIGIYIRRHLNETLPGVEDGA
AGSQPGSQPVSQPVRKLFREHSRVITTGVLTTIGGTAANYIVLFYLSTYAIRILHLPMSS
ALWAAWTAAVVTVICSPFAGALSDRVGRKRVLWVSRVLLIAAVYPAFMMINASPTVPVLL
AVVAGLAVLVAFTAVPNIVMLPEMFPREIRATGMSIVYCLGVSIFGGFAQFFATWLIQVS
GSNLAPAWYLIGCGVVSLLPLPFMRETAGRDIG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory