SitesBLAST
Comparing BPHYT_RS08325 BPHYT_RS08325 glucose dehydrogenase to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
Q4W6G0 Quinohemoprotein alcohol dehydrogenase ADH-IIG; ADH IIG; Alcohol dehydrogenase (azurin); EC 1.1.9.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
25% identity, 85% coverage: 115:826/837 of query aligns to 2:577/718 of Q4W6G0
- C138 (≠ A308) modified: Disulfide link with 139
- C139 (≠ S309) modified: Disulfide link with 138
- R144 (≠ D327) binding
- T189 (≠ A376) binding
- GA 205:206 (≠ SV 397:398) binding
- E207 (= E399) binding
- T264 (≠ N448) binding
- N284 (≠ T470) binding
- D329 (= D512) binding
- K356 (= K540) binding
- W415 (≠ L634) binding
- DW 419:420 (≠ EW 638:639) binding
Sites not aligning to the query:
- 1:29 signal peptide
- 30:718 modified: mature protein, Quinohemoprotein alcohol dehydrogenase ADH-IIG
- 635 binding covalent
- 638 binding covalent
- 639 binding axial binding residue
- 676 binding axial binding residue
1yiqA Molecular cloning and structural analysis of quinohemoprotein alcohol dehydrogenase adhiig from pseudomonas putida hk5. Compariison to the other quinohemoprotein alcohol dehydrogenase adhiib found in the same microorganism. (see paper)
25% identity, 79% coverage: 164:826/837 of query aligns to 18:548/684 of 1yiqA
- active site: E178 (= E399), N255 (≠ T470), D300 (= D512)
- binding calcium ion: E178 (= E399), N255 (≠ T470), D300 (= D512)
- binding pyrroloquinoline quinone: E63 (= E217), C109 (≠ A308), C110 (≠ S309), R115 (≠ D327), T160 (≠ A376), G175 (= G396), G176 (≠ S397), A177 (≠ V398), E178 (= E399), T235 (≠ N448), W237 (= W450), K327 (= K540), D390 (≠ E638), W391 (= W639), F477 (≠ R739), A542 (≠ S820)
Sites not aligning to the query:
- binding heme c: 605, 606, 608, 609, 610, 623, 626, 630, 634, 637, 638, 642, 645, 646, 647, 648, 650
1lrwA Crystal structure of methanol dehydrogenase from p. Denitrificans (see paper)
23% identity, 78% coverage: 164:816/837 of query aligns to 10:536/600 of 1lrwA
- active site: E177 (≠ N401), N261 (≠ V468), D303 (= D512)
- binding calcium ion: E177 (≠ N401), N261 (≠ V468), D303 (= D512)
- binding pyrroloquinoline quinone: E55 (= E217), C103 (= C265), C104 (≠ R266), R109 (≠ V271), T159 (= T374), S174 (≠ V398), G175 (≠ E399), A176 (≠ D400), E177 (≠ N401), T241 (≠ N448), W243 (= W450), R331 (≠ K540), W476 (≠ L755)
Sites not aligning to the query:
P12293 Methanol dehydrogenase [cytochrome c] subunit 1; MDH large subunit alpha; MEDH; EC 1.1.2.7 from Paracoccus denitrificans (see 2 papers)
23% identity, 78% coverage: 164:816/837 of query aligns to 42:567/631 of P12293
- C135 (= C265) modified: Disulfide link with 136
- C136 (≠ R266) modified: Disulfide link with 135
- C418 (≠ F630) modified: Disulfide link with 447
- C447 (≠ A657) modified: Disulfide link with 418
Sites not aligning to the query:
6damA Crystal structure of lanthanide-dependent methanol dehydrogenase xoxf from methylomicrobium buryatense 5g (see paper)
27% identity, 47% coverage: 164:556/837 of query aligns to 10:344/563 of 6damA
- active site: E171 (≠ N401), N259 (≠ T470), D301 (= D512)
- binding pyrroloquinoline quinone: E55 (= E217), C103 (≠ A298), C104 (≠ S299), R109 (≠ A304), T153 (≠ P381), S168 (≠ V398), G169 (≠ E399), G170 (≠ D400), E171 (≠ N401), T239 (≠ N448), W241 (= W450), D303 (= D514), R328 (≠ K540)
Sites not aligning to the query:
Q8GR64 Quinohemoprotein alcohol dehydrogenase ADH IIB; ADH IIB; Alcohol dehydrogenase (azurin); EC 1.1.9.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 3 papers)
24% identity, 78% coverage: 167:816/837 of query aligns to 39:543/690 of Q8GR64
- E81 (= E217) binding
- C127 (≠ A287) modified: Disulfide link with 128
- C128 (≠ S288) modified: Disulfide link with 127
- R133 (≠ T293) binding
- T177 (≠ A376) binding
- GA 193:194 (≠ SV 397:398) binding
- E195 (= E399) binding
- T252 (vs. gap) binding
- N272 (≠ V468) binding
- D317 (= D512) binding
- K344 (= K540) binding
- NW 404:405 (≠ EW 638:639) binding
Sites not aligning to the query:
- 1:22 signal peptide
- 23:690 modified: mature protein, Quinohemoprotein alcohol dehydrogenase ADH IIB
- 547 binding
- 613 binding covalent
- 616 binding covalent
- 617 binding axial binding residue
- 655 binding axial binding residue
1kv9A Structure at 1.9 a resolution of a quinohemoprotein alcohol dehydrogenase from pseudomonas putida hk5 (see paper)
24% identity, 78% coverage: 167:816/837 of query aligns to 17:521/664 of 1kv9A
- active site: E173 (= E399), N250 (≠ V468), D295 (= D512)
- binding acetone: E173 (= E399), D295 (= D512)
- binding calcium ion: E173 (= E399), N250 (≠ V468), D295 (= D512)
- binding heme c: A101 (= A283), R102 (≠ T284)
- binding pyrroloquinoline quinone: E59 (= E217), C105 (≠ A287), C106 (≠ S288), R111 (≠ T293), T155 (≠ A376), G170 (= G396), A172 (≠ V398), E173 (= E399), T230 (vs. gap), W232 (vs. gap), K322 (= K540), N382 (≠ E638), W383 (= W639), W460 (≠ M750)
Sites not aligning to the query:
- binding heme c: 590, 591, 594, 595, 605, 606, 608, 611, 615, 619, 623, 631, 633, 636
- binding pyrroloquinoline quinone: 525
2d0vA Crystal structure of methanol dehydrogenase from hyphomicrobium denitrificans (see paper)
23% identity, 78% coverage: 167:816/837 of query aligns to 13:536/597 of 2d0vA
- active site: E177 (= E399), N261 (≠ V468), D303 (= D512)
- binding calcium ion: E177 (= E399), N261 (≠ V468), D303 (= D512)
- binding pyrroloquinoline quinone: E55 (= E217), R109 (≠ A318), T159 (= T374), S174 (≠ G396), G175 (≠ S397), A176 (≠ V398), E177 (= E399), T241 (≠ N448), W243 (= W450), R331 (≠ K540), N394 (≠ E638), W476 (≠ L755)
Sites not aligning to the query:
5xm3A Crystal structure of methanol dehydrogenase from methylophaga aminisulfidivorans (see paper)
23% identity, 78% coverage: 167:816/837 of query aligns to 13:536/596 of 5xm3A
- active site: E177 (≠ N401), N261 (≠ V468), D303 (= D512)
- binding magnesium ion: E177 (≠ N401), N261 (≠ V468)
- binding pyrroloquinoline quinone: E55 (= E217), C103 (= C265), R109 (≠ T301), T159 (≠ P381), S174 (≠ V398), G175 (≠ E399), A176 (≠ D400), E177 (≠ N401), T241 (≠ N448), W243 (= W450), R331 (≠ K540), N394 (≠ E638), W476 (≠ L755)
Sites not aligning to the query:
6oc6A Lanthanide-dependent methanol dehydrogenase xoxf from methylobacterium extorquens, in complex with lanthanum and pyrroloquinoline quinone (see paper)
23% identity, 77% coverage: 178:825/837 of query aligns to 24:542/579 of 6oc6A
- active site: E171 (≠ H344), N255 (≠ T470), D297 (= D512)
- binding pyrroloquinoline quinone: E55 (= E217), C103 (= C265), C104 (vs. gap), R109 (= R266), T153 (≠ A318), S168 (≠ N341), G169 (≠ D342), G170 (= G343), E171 (≠ H344), W237 (= W450), D299 (= D514), R324 (≠ K540), D395 (≠ L613), W473 (≠ R749), G536 (= G819), W537 (≠ S820)
4aahA Methanol dehydrogenase from methylophilus w3a1 (see paper)
23% identity, 75% coverage: 181:811/837 of query aligns to 27:522/571 of 4aahA
- active site: E171 (≠ Y337), N255 (≠ V468), D297 (= D512)
- binding calcium ion: E171 (≠ Y337), N255 (≠ V468)
- binding pyrroloquinoline quinone: E55 (= E217), C103 (= C265), C104 (≠ R266), R109 (≠ L273), S168 (≠ R334), A170 (≠ I336), E171 (≠ Y337), W237 (= W450), R324 (≠ K540), N387 (≠ E638), W467 (≠ L755)
Sites not aligning to the query:
1w6sC The high resolution structure of methanol dehydrogenase from methylobacterium extorquens (see paper)
22% identity, 78% coverage: 167:816/837 of query aligns to 13:536/596 of 1w6sC
- active site: E177 (= E399), N261 (≠ T470), D303 (= D512)
- binding calcium ion: E177 (= E399), N261 (≠ T470)
- binding pyrroloquinoline quinone: E55 (= E217), C103 (≠ Y270), C104 (≠ V271), R109 (≠ G276), T159 (= T374), S174 (≠ G396), A176 (≠ V398), E177 (= E399), T241 (≠ N448), W243 (= W450), R331 (≠ K540), N394 (≠ E638), W476 (≠ L755)
Sites not aligning to the query:
P16027 Methanol dehydrogenase [cytochrome c] subunit 1; MDH large subunit alpha; MEDH; EC 1.1.2.7 from Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1) (Methylobacterium extorquens) (see 3 papers)
22% identity, 78% coverage: 167:816/837 of query aligns to 40:563/626 of P16027
- C130 (≠ Y270) modified: Disulfide link with 131; mutation to S: Inactive.
- C131 (≠ V271) modified: Disulfide link with 130; mutation to S: Inactive.
- D330 (= D512) mutation to E: Lower affinity for methanol.
- C413 (≠ M609) modified: Disulfide link with 442
- C442 (≠ A657) modified: Disulfide link with 413
Sites not aligning to the query:
7o6zB Structure of a neodymium-containing, xoxf1-type methanol dehydrogenase (see paper)
24% identity, 47% coverage: 164:555/837 of query aligns to 10:356/588 of 7o6zB
- binding methanol: E173 (≠ N401), W263 (= W474), D314 (= D512)
- binding Neodymium Ion: E173 (≠ N401), N259 (≠ T470), D314 (= D512), D316 (= D514)
- binding pyrroloquinoline quinone: E55 (= E217), C105 (≠ T264), C106 (= C265), R111 (≠ A304), T155 (≠ S383), G170 (≠ V398), G171 (≠ E399), D172 (= D400), E173 (≠ N401), W241 (= W450), D316 (= D514), R341 (≠ K540)
Sites not aligning to the query:
7o6zA Structure of a neodymium-containing, xoxf1-type methanol dehydrogenase (see paper)
24% identity, 47% coverage: 164:555/837 of query aligns to 10:356/588 of 7o6zA
Sites not aligning to the query:
6fkwA Europium-containing methanol dehydrogenase (see paper)
25% identity, 47% coverage: 164:559/837 of query aligns to 10:345/576 of 6fkwA
- active site: E172 (≠ N401), N256 (≠ T470), D299 (= D512), D301 (= D514)
- binding europium ion: E172 (≠ N401), N256 (≠ T470), D299 (= D512), D301 (= D514)
- binding pyrroloquinoline quinone: E55 (= E217), C104 (≠ P297), C105 (≠ A298), R110 (≠ T303), T154 (≠ P381), S169 (≠ V398), G170 (≠ E399), G171 (≠ D400), E172 (≠ N401), T236 (≠ N448), W238 (= W450), D301 (= D514), R326 (≠ K540)
Sites not aligning to the query:
4maeA Methanol dehydrogenase from methylacidiphilum fumariolicum solv (see paper)
25% identity, 47% coverage: 164:559/837 of query aligns to 10:345/577 of 4maeA
- active site: E172 (≠ N401), N256 (≠ T470), D299 (= D512)
- binding cerium (iii) ion: E172 (≠ N401), N256 (≠ T470), D299 (= D512), D301 (= D514)
- binding pyrroloquinoline quinone: E55 (= E217), C104 (≠ P297), C105 (≠ A298), R110 (≠ T303), T154 (≠ P381), S169 (≠ V398), G170 (≠ E399), G171 (≠ D400), E172 (≠ N401), T236 (≠ N448), W238 (= W450), D301 (= D514), R326 (≠ K540)
Sites not aligning to the query:
7ce5A Methanol-pqq bound methanol dehydrogenase (mdh) from methylococcus capsulatus (bath) (see paper)
23% identity, 47% coverage: 164:555/837 of query aligns to 10:345/573 of 7ce5A
- active site: E177 (= E399), N261 (≠ V468), D303 (= D512)
- binding calcium ion: E177 (= E399), N261 (≠ V468), D303 (= D512)
- binding methanol: E177 (= E399)
- binding pyrroloquinoline quinone: E55 (= E217), C103 (≠ A295), C104 (≠ A296), R109 (≠ T301), T159 (= T374), A174 (≠ G396), G175 (≠ S397), A176 (≠ V398), E177 (= E399), T241 (≠ N448), W243 (= W450), R330 (≠ K540)
Sites not aligning to the query:
7cdlC Holo-methanol dehydrogenase (mdh) with cys131-cys132 reduced from methylococcus capsulatus (bath) (see paper)
23% identity, 47% coverage: 164:555/837 of query aligns to 10:345/573 of 7cdlC
- active site: E177 (= E399), N261 (≠ V468), D303 (= D512)
- binding calcium ion: E177 (= E399), N261 (≠ V468), D303 (= D512)
- binding pyrroloquinoline quinone: E55 (= E217), R109 (≠ T301), T159 (= T374), A174 (≠ G396), A176 (≠ V398), E177 (= E399), T241 (≠ N448), W243 (= W450), D303 (= D512), R330 (≠ K540)
Sites not aligning to the query:
1flgA Crystal structure of the quinoprotein ethanol dehydrogenase from pseudomonas aeruginosa (see paper)
26% identity, 46% coverage: 171:552/837 of query aligns to 21:356/582 of 1flgA
- active site: E179 (vs. gap), N266 (≠ E440), D316 (= D512)
- binding calcium ion: E179 (vs. gap), N266 (≠ E440), D316 (= D512)
- binding pyrroloquinoline quinone: E61 (= E217), C105 (≠ S259), C106 (≠ F260), R111 (≠ C265), T155 (= T317), S176 (≠ V340), G177 (≠ N341), D178 (= D342), W248 (≠ L422), R344 (≠ K540)
Sites not aligning to the query:
Query Sequence
>BPHYT_RS08325 BPHYT_RS08325 glucose dehydrogenase
MTSQSNSRGVIGVVTLLFTVLTALYLLIGGAWLLSVGGSAYYLITGVALLGVAWLLWRRS
PTALVLYSLVLIGTAIWALMESGPDFWALAPRSGVLVIFGVWLLLLVSWRLVGERKLGVV
SLVVALVAWAGVLVYASFNDPQQVNGTLSASAPASGAGAGSGIDPADWPAYGRTQEGTRY
SPLQQITPENVKDLQVAWTFRTGDMKGPNDPVEITDEVTPIKIGDLLYLCSPHQILFALD
AKTGTLKWKFDPRLKSDPSFQHVTCRGVSYVDLSAGATAAAPATAPASDATATAAAPASD
TATAAAPASDTATATTTAAPAATPASDTTATCTRRIYLPVNDGHLYALDALTGQRCEGFA
NHGDLDLQHAQPVTTAGMYEPTSPSIITSKVIIVAGSVEDNFSNREPSGVIRGFDVRTGE
LLWAFDPGAKDPNHIPGAGEHYTWNSPNSWAPAAYDAKLDIVYLPMGVTTPDIWGGNRTP
EQERYASGLLALNASTGKLAWFYQTAHHDLWDMDQPSQPTLADITGKDGQSVPVVYAPAK
TGNLFVLDRRTGVPVVPAPETPVPQGAAPGDHVSPTQPFSQLTYRPSKKLTDADMWGATM
YDQLVCRVMFHKLRYEGTFTPPSLQGTLVFPGNLGMFEWGGIAVDTDRQIAVANPIALPF
VSRLIPRGPGNPLEPVPGAKGSGTESGIQPQYGVPYGVVINAFLSPFGLPCKQPAWGYIS
AIDLKTNEIVWKKRIGTVRDSSPIPLPFRMGMPMLGGPIVTAGGVAFIGATADNYIRGFD
VNNGKQVWEARLPAGGQATPMSYSINGRQYLVIAAGGHGSFGTKLGDYVIAYALPQQ
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory