SitesBLAST
Comparing BPHYT_RS09280 FitnessBrowser__BFirm:BPHYT_RS09280 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q7XSN8 Serine racemase; D-serine dehydratase; D-serine ammonia-lyase; L-serine dehydratase; L-serine ammonia-lyase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Oryza sativa subsp. japonica (Rice) (see paper)
34% identity, 85% coverage: 31:324/344 of query aligns to 31:328/339 of Q7XSN8
- E219 (= E218) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-225.
- D225 (≠ V224) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-219.
5cvcA Structure of maize serine racemase (see paper)
33% identity, 92% coverage: 21:337/344 of query aligns to 5:329/329 of 5cvcA
- active site: K52 (= K68), S77 (≠ G93), E203 (= E218), A207 (= A222), D209 (≠ V224), G231 (≠ S246), V306 (≠ L318), S307 (≠ C319)
- binding magnesium ion: E203 (= E218), A207 (= A222), D209 (≠ V224)
- binding pyridoxal-5'-phosphate: F51 (= F67), K52 (= K68), N79 (= N95), S178 (≠ G193), G179 (= G194), G180 (= G195), G181 (= G196), L232 (= L247), E275 (= E291), S307 (≠ C319), G308 (= G320)
A4F2N8 L-threo-3-hydroxyaspartate ammonia-lyase; L-threo-3-hydroxyaspartate dehydratase; L-THA DH; EC 4.3.1.16 from Pseudomonas sp. (see paper)
35% identity, 88% coverage: 31:333/344 of query aligns to 16:318/319 of A4F2N8
- K53 (= K68) mutation to A: Loss of enzymatic activity.
O59791 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 4.3.1.17; EC 4.3.1.18; EC 5.1.1.18 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
33% identity, 95% coverage: 14:341/344 of query aligns to 3:323/323 of O59791
- S82 (≠ G93) mutation to A: Loss of racemase activity. Reduces D-serine dehydratase activity by 99%. Slightly reduced L-serine dehydratase activity.
2zr8A Crystal structure of modified serine racemase complexed with serine (see paper)
33% identity, 94% coverage: 18:341/344 of query aligns to 3:319/319 of 2zr8A
- active site: K53 (= K68), S78 (≠ G93), E204 (= E218), G208 (≠ A222), D210 (≠ V224), G232 (≠ S246), I303 (≠ L318), S304 (≠ C319)
- binding magnesium ion: E204 (= E218), G208 (≠ A222), D210 (≠ V224)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F67), K53 (= K68), S77 (= S92), S78 (≠ G93), N80 (= N95), H81 (= H96), P147 (= P161), G179 (= G193), G180 (= G194), G181 (= G195), G182 (= G196), G232 (≠ S246), E277 (= E291), T279 (≠ A293), S304 (≠ C319)
- binding serine: S78 (≠ G93), R129 (≠ F144), D231 (= D245), G232 (≠ S246), A233 (≠ L247), Q234 (≠ M248), T235 (≠ S249)
2zpuA Crystal structure of modified serine racemase from s.Pombe. (see paper)
33% identity, 94% coverage: 18:341/344 of query aligns to 3:319/319 of 2zpuA
- active site: K53 (= K68), S78 (≠ G93), E204 (= E218), G208 (≠ A222), D210 (≠ V224), G232 (≠ S246), I303 (≠ L318), S304 (≠ C319)
- binding magnesium ion: E204 (= E218), G208 (≠ A222), D210 (≠ V224)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F67), K53 (= K68), S77 (= S92), S78 (≠ G93), N80 (= N95), H81 (= H96), P147 (= P161), G179 (= G193), G180 (= G194), G181 (= G195), G182 (= G196), G232 (≠ S246), E277 (= E291), T279 (≠ A293), S304 (≠ C319)
1wtcA Crystal structure of s.Pombe serine racemase complex with amppcp (see paper)
33% identity, 94% coverage: 18:341/344 of query aligns to 2:318/318 of 1wtcA
- active site: K52 (= K68), S77 (≠ G93), E203 (= E218), G207 (≠ A222), D209 (≠ V224), G231 (≠ S246), I302 (≠ L318), S303 (≠ C319)
- binding phosphomethylphosphonic acid adenylate ester: N20 (≠ A36), K47 (≠ A63), M48 (≠ G64), A109 (= A125), A110 (≠ L126), Y114 (= Y130)
- binding magnesium ion: E203 (= E218), G207 (≠ A222), D209 (≠ V224)
- binding pyridoxal-5'-phosphate: F51 (= F67), K52 (= K68), N79 (= N95), G178 (= G193), G179 (= G194), G180 (= G195), G181 (= G196), G231 (≠ S246), E276 (= E291), T278 (≠ A293), S303 (≠ C319)
1v71A Crystal structure of s.Pombe serine racemase
33% identity, 94% coverage: 18:341/344 of query aligns to 2:318/318 of 1v71A
- active site: K52 (= K68), S77 (≠ G93), E203 (= E218), G207 (≠ A222), D209 (≠ V224), G231 (≠ S246), I302 (≠ L318), S303 (≠ C319)
- binding magnesium ion: E203 (= E218), G207 (≠ A222), D209 (≠ V224)
- binding pyridoxal-5'-phosphate: F51 (= F67), K52 (= K68), N79 (= N95), G178 (= G193), G179 (= G194), G180 (= G195), G181 (= G196), G231 (≠ S246), E276 (= E291), T278 (≠ A293), S303 (≠ C319), G304 (= G320)
2gn2A Crystal structure of tetrameric biodegradative threonine deaminase (tdcb) from salmonella typhimurium in complex with cmp at 2.5a resolution (hexagonal form) (see paper)
35% identity, 78% coverage: 56:324/344 of query aligns to 44:314/326 of 2gn2A
- active site: K56 (= K68), A81 (≠ G93), Q207 (≠ E218), V211 (≠ A222), G213 (≠ V224), G235 (≠ S246), I308 (≠ L318), S309 (≠ C319)
- binding cytidine-5'-monophosphate: R51 (≠ A63), T52 (≠ G64), G53 (= G65), A114 (≠ L126), D117 (≠ Q129), Y118 (= Y130), N312 (= N322)
3l6bA X-ray crystal structure of human serine racemase in complex with malonate a potent inhibitor (see paper)
29% identity, 91% coverage: 23:335/344 of query aligns to 9:322/322 of 3l6bA
- active site: K54 (= K68), S77 (≠ G93), E203 (= E218), A207 (= A222), D209 (≠ V224), G232 (≠ S246), T278 (≠ A293), L305 (= L318), S306 (≠ C319)
- binding malonate ion: K54 (= K68), S76 (= S92), S77 (≠ G93), N79 (= N95), H80 (= H96), R128 (≠ F144), G232 (≠ S246)
- binding manganese (ii) ion: E203 (= E218), A207 (= A222), D209 (≠ V224)
- binding pyridoxal-5'-phosphate: F53 (= F67), K54 (= K68), N79 (= N95), G178 (= G193), G179 (= G194), G180 (= G195), G181 (= G196), M182 (≠ L197), V233 (≠ L247), E276 (= E291), T278 (≠ A293), S306 (≠ C319), G307 (= G320)
7nbgDDD structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
29% identity, 88% coverage: 23:324/344 of query aligns to 8:310/310 of 7nbgDDD
- active site: K53 (= K68), S76 (≠ G93), E202 (= E218), A206 (= A222), D208 (≠ V224), G231 (≠ S246), L304 (= L318), S305 (≠ C319)
- binding calcium ion: E202 (= E218), A206 (= A222), D208 (≠ V224)
- binding magnesium ion: N239 (≠ Y255)
- binding ortho-xylene: S76 (≠ G93), Q81 (≠ V98), I96 (≠ V113), Y113 (= Y130)
- binding pyridoxal-5'-phosphate: F52 (= F67), K53 (= K68), N78 (= N95), G177 (= G193), G178 (= G194), G179 (= G195), G180 (= G196), M181 (≠ L197), G231 (≠ S246), V232 (≠ L247), E275 (= E291), T277 (≠ A293), S305 (≠ C319), G306 (= G320)
Sites not aligning to the query:
6zspAAA serine racemase bound to atp and malonate. (see paper)
29% identity, 91% coverage: 23:336/344 of query aligns to 8:320/320 of 6zspAAA
- active site: K53 (= K68), S74 (≠ G93), E200 (= E218), A204 (= A222), D206 (≠ V224), G229 (≠ S246), L302 (= L318), S303 (≠ C319)
- binding adenosine-5'-triphosphate: S28 (≠ R43), S29 (≠ V44), I30 (≠ D45), K48 (≠ A63), T49 (≠ G64), Q79 (≠ V98), Y111 (= Y130), E266 (≠ G284), R267 (≠ Q285), K269 (= K287), N306 (= N322)
- binding magnesium ion: E200 (= E218), A204 (= A222), D206 (≠ V224)
- binding malonate ion: K53 (= K68), S73 (= S92), S74 (≠ G93), N76 (= N95), H77 (= H96), R125 (≠ F144), G229 (≠ S246), S232 (= S249)
7nbhAAA structure of human serine racemase in complex with DSiP fragment Z26781964, XChem fragment screen (see paper)
30% identity, 88% coverage: 23:324/344 of query aligns to 8:315/320 of 7nbhAAA
- active site: K53 (= K68), S81 (≠ G93), E207 (= E218), A211 (= A222), D213 (≠ V224), G236 (≠ S246), L309 (= L318), S310 (≠ C319)
- binding calcium ion: E207 (= E218), A211 (= A222), D213 (≠ V224)
- binding N-[(1H-benzimidazol-2-yl)methyl]furan-2-carboxamide: S81 (≠ G93), G85 (≠ A97), Q86 (≠ V98), K111 (≠ R123), I115 (≠ C127), Y118 (= Y130), D235 (= D245), P281 (= P292), N313 (= N322), V314 (≠ T323), D315 (= D324)
7nbgAAA structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
30% identity, 88% coverage: 23:324/344 of query aligns to 8:315/322 of 7nbgAAA
- active site: K53 (= K68), S81 (≠ G93), E207 (= E218), A211 (= A222), D213 (≠ V224), G236 (≠ S246), L309 (= L318), S310 (≠ C319)
- binding calcium ion: E207 (= E218), A211 (= A222), D213 (≠ V224)
- binding pyridoxal-5'-phosphate: F52 (= F67), K53 (= K68), N83 (= N95), G182 (= G193), G183 (= G194), G184 (= G195), G185 (= G196), M186 (≠ L197), G236 (≠ S246), V237 (≠ L247), T282 (≠ A293), S310 (≠ C319), G311 (= G320)
- binding ~{N}-[2-(2-methylphenyl)ethyl]ethanamide: S81 (≠ G93), G85 (≠ A97), Q86 (≠ V98), I101 (≠ V113), K111 (≠ R123), I115 (≠ C127), Y118 (= Y130)
7nbfAAA structure of human serine racemase in complex with DSiP fragment Z126932614, XChem fragment screen (see paper)
30% identity, 88% coverage: 23:324/344 of query aligns to 8:315/323 of 7nbfAAA
- active site: K53 (= K68), S81 (≠ G93), E207 (= E218), A211 (= A222), D213 (≠ V224), G236 (≠ S246), L309 (= L318), S310 (≠ C319)
- binding calcium ion: E207 (= E218), A211 (= A222), D213 (≠ V224)
- binding magnesium ion: N244 (≠ Y255)
- binding pyridoxal-5'-phosphate: F52 (= F67), K53 (= K68), N83 (= N95), G182 (= G193), G183 (= G194), G184 (= G195), G185 (= G196), M186 (≠ L197), G236 (≠ S246), V237 (≠ L247), T282 (≠ A293), S310 (≠ C319), G311 (= G320)
- binding 2-[(methylsulfonyl)methyl]-1H-benzimidazole: H21 (≠ A36), L22 (≠ R37), T23 (= T38), P24 (= P39), L26 (≠ F41), T27 (≠ D42), F46 (≠ L61)
Sites not aligning to the query:
7nbdAAA structure of human serine racemase in complex with DSiP fragment Z235449082, XChem fragment screen (see paper)
30% identity, 88% coverage: 23:324/344 of query aligns to 8:315/323 of 7nbdAAA
- active site: K53 (= K68), S81 (≠ G93), E207 (= E218), A211 (= A222), D213 (≠ V224), G236 (≠ S246), L309 (= L318), S310 (≠ C319)
- binding calcium ion: E207 (= E218), A211 (= A222), D213 (≠ V224)
- binding [4-(1H-benzimidazol-1-yl)phenyl]methanol: W272 (≠ F283), L278 (≠ A289), V314 (≠ T323)
- binding magnesium ion: N244 (≠ Y255)
- binding pyridoxal-5'-phosphate: F52 (= F67), K53 (= K68), N83 (= N95), G182 (= G193), G183 (= G194), G184 (= G195), G185 (= G196), M186 (≠ L197), G236 (≠ S246), V237 (≠ L247), E280 (= E291), T282 (≠ A293), S310 (≠ C319), G311 (= G320)
Sites not aligning to the query:
7nbcCCC structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
30% identity, 88% coverage: 23:324/344 of query aligns to 8:315/323 of 7nbcCCC
- active site: K53 (= K68), S81 (≠ G93), E207 (= E218), A211 (= A222), D213 (≠ V224), G236 (≠ S246), L309 (= L318), S310 (≠ C319)
- binding biphenyl-4-ylacetic acid: T78 (≠ C90), H79 (≠ V91), H84 (= H96), V148 (= V159), H149 (= H160), P150 (= P161)
- binding calcium ion: E207 (= E218), A211 (= A222), D213 (≠ V224)
- binding pyridoxal-5'-phosphate: F52 (= F67), K53 (= K68), N83 (= N95), G182 (= G193), G183 (= G194), G184 (= G195), G185 (= G196), M186 (≠ L197), G236 (≠ S246), V237 (≠ L247), T282 (≠ A293), S310 (≠ C319), G311 (= G320)
7nbcAAA structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
30% identity, 88% coverage: 23:324/344 of query aligns to 8:315/323 of 7nbcAAA
- active site: K53 (= K68), S81 (≠ G93), E207 (= E218), A211 (= A222), D213 (≠ V224), G236 (≠ S246), L309 (= L318), S310 (≠ C319)
- binding calcium ion: E207 (= E218), A211 (= A222), D213 (≠ V224)
- binding magnesium ion: N244 (≠ Y255)
- binding pyridoxal-5'-phosphate: F52 (= F67), K53 (= K68), N83 (= N95), G182 (= G193), G183 (= G194), G184 (= G195), G185 (= G196), M186 (≠ L197), G236 (≠ S246), V237 (≠ L247), T282 (≠ A293), S310 (≠ C319), G311 (= G320)
Sites not aligning to the query:
1ve5A Crystal structure of t.Th. Hb8 threonine deaminase
36% identity, 89% coverage: 19:324/344 of query aligns to 1:305/308 of 1ve5A
- active site: K50 (= K68), S56 (≠ T74), S72 (≠ G93), E200 (= E218), A204 (= A222), D206 (≠ V224), G229 (≠ S246), L299 (= L318), S300 (≠ C319)
- binding calcium ion: E200 (= E218), A204 (= A222), D206 (≠ V224)
- binding pyridoxal-5'-phosphate: F49 (= F67), K50 (= K68), N74 (= N95), G175 (= G193), G176 (= G194), G177 (= G195), G178 (= G196), E274 (= E291), T276 (≠ A293), S300 (≠ C319), G301 (= G320)
P04968 L-threonine dehydratase biosynthetic IlvA; Threonine deaminase; EC 4.3.1.19 from Escherichia coli (strain K12) (see paper)
31% identity, 84% coverage: 35:324/344 of query aligns to 20:320/514 of P04968
- K62 (= K68) modified: N6-(pyridoxal phosphate)lysine
- N89 (= N95) binding
- GGGGL 188:192 (= GGGGL 193:197) binding
- S315 (≠ C319) binding
Query Sequence
>BPHYT_RS09280 FitnessBrowser__BFirm:BPHYT_RS09280
MSTATPQHTDHTIDGEPIPTLDDIAAQHFALTPWVARTPVFDRVDFPSLEGTVVNFKFEL
LQAGGSFKARGAFTNLLALDEAQRSAGVTCVSGGNHAVAVAYAAMRLGVSAKVVLFRAAN
PARVALCRQYRAEIVFAEDIAEAFELVRRIEAEEGRYFVHPFNGYRTVLGSATLGYEWAT
QTPDLEAVILPIGGGGLAAGVATAMRLANPNVHVYGVEPEGADVMGKSFAANHTVKMRQM
RGIADSLMSPHTEQYSYELCRRHIDQLVTVSDDQLRAAMLTLFGQLKLAVEPACAAATAG
LLGPLREQLQGKRVGVLLCGTNTDPVTFAAHIERARHSESQFPQ
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory