SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS09400 BPHYT_RS09400 spermidine/putrescine ABC transporter ATPase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
50% identity, 79% coverage: 3:285/359 of query aligns to 17:292/378 of P69874

query
sites
P69874

F

L

L

V

T

Q

L

L

Q

A

G

G

I

I

S

R

K

K

R
x
C

Y
x
F

G

D

D

G

F

K

T

E

A

V

I

I

E

P

Q

Q

L

L

D

D

L

L

A

T

V

I

E

N

R

N

G

G

E

E

L
x
F

L

L

S

T

L

L

G

G

P

P

S

S

G

G

C
|
C

G

G

K

K

T

T

T

V

L
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L

Q

R

M

L

V

I

A

A

G

G

F

L

I

E

T

T

P

V

T

D

T

S

G

G

R

R

I

I

L

M

L
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L

D

D

G

N

R

E

D

D

I

I

T

T

N

H

E

V

R

P

P

A

E

E

K

N

R

R

G

Y

I

V

G

N

V

T

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

F

P

P

H

H

M

M

T

T

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V

A

F

G

E

N

N

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V

G

A

F

F

G

G

L

L

E

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M

M

R

Q

K

K

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D

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E

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L

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D

A

Y

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K

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M

M

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E

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A

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K

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L

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G

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I

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T

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T

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H

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D

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Q

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E

E

A

A

M

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T

T

M

M

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D

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R

I

I

A

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M

M

H

R

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D

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G

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T

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P

Y

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Y

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K

T

N

P

L

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F

A

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A

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G

F

F

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E

T

I

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N

A

M

F

F

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N

G

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T

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L

I

R

E

R

R

N

-

P

-

R

-

C

-

A

-

E

-

V

L

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D

V

E

A

Q

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R

T

V

T

R

L

A

H

N

V

V

P

-

H

-

E

E

G

G

R

R

H

E

V
x
C

D

N

G

I

T

Y

V

V

N

N

V

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Y

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I

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P

-

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E

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K

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L

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H

L

V

C26 (≠ R12) mutation to A: Lower ATPase activity and transport efficiency.
F27 (≠ Y13) mutation to L: Lower ATPase activity and transport efficiency.
F45 (≠ L31) mutation to L: Lower ATPase activity and transport efficiency.
C54 (= C40) mutation to T: Loss of ATPase activity and transport.
L60 (= L46) mutation to F: Lower ATPase activity and transport efficiency.
L76 (= L62) mutation to P: Lower ATPase activity and transport efficiency.
V135 (= V121) mutation to M: Loss of ATPase activity and transport.
D172 (= D158) mutation to N: Loss of ATPase activity and transport.
C276 (≠ V270) mutation to A: Lower ATPase activity and transport efficiency.

Sites not aligning to the query:

297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
43% identity, 99% coverage: 1:354/359 of query aligns to 4:351/353 of 1vciA

query
sites
1vciA

M

M

A

V

F

E

L

V

T

K

L

L

Q

E

G

N

I

L

S

T

K

K

R

R

Y
x
F

G

G

D

N

F
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F

T

T

A

A

I

V

E

N

Q

K

L

L

D

N

L

L

A

T

V

I

E

K

R

D

G

G

E

E

L

F

L

L

S

V

L

L

G

G

P
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P

S
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S

G
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G

C
|
C

G
|
G

K
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K

T
|
T

T

T

L

L

Q

R

M

M

V

I

A

A

G

G

F

L

I

E

T

E

P

P

T

T

T

E

G

G

R

R

I

I

L

Y

L

F

D

G

G

D

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R

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D

I

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T

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Y

E

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P

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E

K

K

D

R

R

G

N

I

I

G

S

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M

V

V

F

F

Q

Q

S

-

Y

-

A

-

L

-

F

-

P

-

H

H

M

M

T

T

V

V

A

Y

G

E

N

N

V

I

G

A

F

F

G

P

L

L

E

-

M

-

R

K

K

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F

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P

R

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K

D

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E

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I

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D

E

K

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V

A

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E

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A

A

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A

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G

E

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L

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Y

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P

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A

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L

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S

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G

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A

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A

A

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E

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E

D

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V

L

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M

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D

E

E

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P

M

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S

N

N

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L

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D

A

A

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M

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T

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E

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A

M

M

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T

M

M

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D

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I

A

A

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M

M

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N

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R

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T

S

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P

Y

T

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E

A

V

Y

Y

E

L

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R

P

P

A

N

T

S

P

V

F

F

A

V

S

A

T

T

F

F

L

I

G

G

R

-

T

-

N

-

A

A

F

P

S

E

G

M

E

N

V

I

L

L

R

E

R

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N

S

P

V

R

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C

D

A

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Y

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L

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E

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A

R

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G

T

F

T

R

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H

E

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L

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P

H

Q

E

M

G

D

-

L

-

K

R

D

H

Y

V

V

D

G

G

K

T

T

V

V

N

L

V

F

Y

G

I

I

R

R

P

P

E

E

K

H

-

M

-

T

-

V

-

E

-

G

V

V

H

H

L

M

A

K

N

R

G

-

D

T

A

A

R

R

L

L

R

I

G

G

R

K

I

V

A

D

T

F

R

V

V

E

F

A

V

L

G

G

N

T

Q

D

W

T

L

I

L

L

E

H

V

V

D

K

T

F

E

G

L

D

G

E

K

L

L

V

R

K

I

V

A

K

Q

L

P

P

N

G

H

H

G

-

A

-

P

I

P

P

P

I

E

E

E

P

G

G

H

R

E

E

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V

G

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L

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M

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L

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M

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R

H

V

V

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F

T

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K

D

D

T

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P

E

E

K

S

A

binding adenosine-5'-triphosphate: F16 (≠ Y13), F19 (= F16), P40 (= P37), S41 (= S38), G42 (= G39), C43 (= C40), G44 (= G41), K45 (= K42), T46 (= T43), T47 (= T44)

2d62A Crystal structure of multiple sugar binding transport atp- binding protein
52% identity, 65% coverage: 1:235/359 of query aligns to 4:244/375 of 2d62A

query
sites
2d62A

M

M

A

A

F

E

L

V

T

K

L

L

Q

I

G

N

I

I

S

W

K

K

R

R

Y

F

G

G

D

D

F

V

T

T

A

A

I

V

E

K

Q

D

L

L

D

S

L

L

A

E

V

I

E

K

R

D

G

G

E

E

L

F

L

L

S

V

L

L

G

G

P
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P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
|
T

T

T

L

L

Q

R

M

M

V

I

A

A

G

G

F

L

I

E

T

E

P

P

T

T

T

R

G

G

R

Q

I

I

L

Y

L

I

D

E

G

D

R

N

D

L

I

V

T

A

N

D

E

P

R

E

-

K

-

G

-

V

-

F

-

V

-

P

P

P

E

K

K

E

R

R

G

D

I

V

G

A

V

M

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

T

T

V

V

A

Y

G

D

N

N

V

I

G

A

F

F

G

P

L

L

E

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M

L

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R

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K

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E

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K

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E

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M

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G

L

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G

E

L

L

G

L

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N

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R

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P

P

K

R

E

E

L

L

S

S

G

G

Q

Q

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R

Q

Q

R

R

V

V

A

A

I

L

A

G

R

R

A

A

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I

A

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M

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R

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P

E

K

L

V

L

F

L

L

L

M

D

D

E

E

P

P

M

L

S

S

N

N

L

L

D

D

A

A

K

K

L

L

R

R

E

V

E

K

M

M

H

R

I

A

E

E

L

L

R

K

A

K

I

L

Q

Q

K

R

R

Q

L

L

G

G

I

V

T

T

I

I

L

Y

V

V

T

T

H

H

D

D

Q

Q

V

V

E

E

A

A

M

M

T

T

M

M

S

G

D

D

R

R

I

I

A

A

V

V

M

M

H

N

R

K

G

G

V

E

I

L

A

Q

Q

Q

L

V

S

G

T

T

P

P

Y

D

D

E

A

V

Y

Y

E

Y

R

K

P

P

A

V

T

N

P

T

F

F

A

V

S

A

T

G

F

F

L

I

G

G

binding pyrophosphate 2-: P40 (= P37), S41 (= S38), G42 (= G39), C43 (= C40), G44 (= G41), K45 (= K42), T46 (= T43), T47 (= T44)

1g291 Malk (see paper)
52% identity, 65% coverage: 1:235/359 of query aligns to 1:241/372 of 1g291

query
sites
1g291

M

M

A

A

F

G

L

V

T

R

L

L

Q

V

G

D

I

V

S

W

K

K

R

V

Y

F

G

G

D

E

F

V

T

T

A

A

I

V

E

R

Q

E

L

M

D

S

L

L

A

E

V

V

E

K

R

D

G

G

E

E

L

F

L

M

S

I

L

L

G

G

P
|
P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
|
T

T

T

L

L

Q

R

M

M

V

I

A

A

G

G

F

L

I

E

T

E

P

P

T

S

T

R

G

G

R

Q

I

I

L

Y

L

I

D

G

G

D

R

K

D

L

I

V

T

A

N
x
D

E

P

R
x
E

-
x
K

-

G

-

I

-

F

-

V

-

P

P

P

E
x
K

K
x
D

R

R

G

D

I

I

G

A

V

M

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

T

T

V

V

A

Y

G

D

N

N

V

I

G

A

F

F

G

P

L

L

E

K

M

L

R

R

K

K

V

V

R

P

R

R

K

Q

E

E

R

I

D

D

E

Q

R

R

V

V

A

R

E

E

A

V

L

A

S

E

L

L

V

L

R

G

L

L

K

T

G

E

L

L

G

L

H

N

R

R

Y

K

P

P

K

R

E

E

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

I

L

A

G

R

R

A

A

I

I

A

V

M

R

Q

K

P

P

E

Q

L

V

L

F

L

L

L

M

D

D

E

E

P

P

M

L

S

S

N

N

L

L

D

D

A

A

K

K

L

L

R

R

E

V

E

R

M

M

H

R

I

A

E

E

L

L

R

K

A

K

I

L

Q

Q

K

R

R

Q

L

L

G

G

I

V

T

T

I

I

L

Y

V

V

T

T

H

H

D

D

Q

Q

V

V

E

E

A

A

M

M

T

T

M

M

S

G

D

D

R

R

I

I

A

A

V

V

M

M

H

N

R

R

G

G

V

V

I

L

A

Q

Q

Q

L

V

S

G

T

S

P

P

Y

D

D

E

A

V

Y
|
Y

E
x
D

R

K

P

P

A

A

T

N

P

T

F

F

A

V

S

A

T

G

F

F

L

I

G

G

binding magnesium ion: D69 (≠ N69), E71 (≠ R71), K72 (vs. gap), K79 (≠ E73), D80 (≠ K74), Y228 (= Y222), D229 (≠ E223)
binding pyrophosphate 2-: P37 (= P37), S38 (= S38), G39 (= G39), C40 (= C40), G41 (= G41), K42 (= K42), T43 (= T43), T44 (= T44)

Sites not aligning to the query:

binding magnesium ion: 292, 293, 341, 342, 359

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
49% identity, 65% coverage: 1:235/359 of query aligns to 1:236/393 of P9WQI3

query
sites
P9WQI3

M

M

A

A

F

E

L

I

T

V

L

L

Q

D

G

H

I

V

S

N

K

K

R

S

Y

Y

G

P

D

D

-

G

F

H

T

T

A

A

I

V

E

R

Q

D

L

L

D

N

L

L

A

T

V

I

E

A

R

D

G

G

E

E

L

F

L

L

S

I

L

L

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

T

L

L

Q

N

M

M

V

I

A

A

G

G

F

L

I

E

T

D

P

I

T

S

G

G

R

E

I

L

L

R

L

I

D

A

G

G

R

E

D

R

I

V

T

N

N

E

E

K

R

A

P

P

E

K

K

D

R

R

G

D

I

I

G

A

V

M

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

T

T

V

V

A

R

G

Q

N

N

V

I

G

A

F

F

G

P

L

L

E

T

M

L

R

A

K

K

V

M

R

R

R

K

K

A

E

D

R

I

D

A

E

Q

R

K

V

V

A

S

E

E

A

T

L

A

S

K

L

I

V

L

R

D

L

L

K

T

G

N

L

L

G

L

H

D

R

R

Y

K

P

P

K

S

E

Q

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

I

M

A

G

R

R

A

A

I

I

A

V

M

R

Q

H

P

P

E

K

L

A

L

F

L

L

L

M

D

D

E

E

P

P

M

L

S

S

N

N

L

L

D

D

A

A

K

K

L

L

R

R

E

V

E

Q

M

M

H

R

I

G

E

E

L

I

R

A

A

Q

I

L

Q

Q

K

R

R

R

L

L

G

G

I

T

T

T

I

V

L

Y

V

V

T

T

H
|
H

D

D

Q

Q

V

T

E

E

A

A

M

M

T

T

M

L

S

G

D

D

R

R

I

V

A

V

V

V

M

M

H

Y

R

G

G

G

V

I

I

A

A

Q

Q

Q

L

I

S

G

T

T

P

P

Y

E

D

E

A

L

Y

Y

E

E

R

R

P

P

A

A

T

N

P

L

F

F

A

V

S

A

T

G

F

F

L

I

G

G

H193 (= H192) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
40% identity, 85% coverage: 1:305/359 of query aligns to 1:313/371 of P68187

query
sites
P68187

M

M

A

A

F

S

L

V

T

Q

L

L

Q

Q

G

N

I

V

S

T

K

K

R

A

Y

W

G

G

D

E

F

V

T

V

A

V

I

S

E

K

Q

D

L

I

D

N

L

L

A

D

V

I

E

H

R

E

G

G

E

E

L

F

L

V

S

V

L

F

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

S

T

T

T

L

L

L

Q

R

M

M

V

I

A

A

G

G

F

L

I

E

T

T

P

I

T

T

T

S

G

G

R

D

I

L

L

F

L

I

D

G

G

E

R

K

D

R

I

M

T

N

N

D

E

T

R

P

P

P

E

A

K

E

R

R

G

G

I

V

G

G

V

M

V

V

F

F

Q

Q

S

S

Y

Y

A
|
A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

A

A

G

E

N

N

V

M

G

S

F

F

G

G

L

L

E

K

M

L

R

A

K

G

V

A

R
x
K

R

K

K

E

E

V

R

I

D

N

E

Q

R

R

V
|
V

A

N

E

Q

A
x
V

L

A

S
x
E

L

V

V

L

R

Q

L

L

K
x
A

G

H

L

L

G

L

H

D

R

R

Y

K

P

P

K

K

E

A

L

L

S

S

G

G

G
|
G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

I

L

A

V

M

A

Q

E

P

P

E

S

L

V

L

F

L

L

D
|
D

E

E

P

P

M

L

S

S

N

N

L

L

D

D

A

A

K

A

L

L

R

R

E

V

E

Q

M

M

H

R

I

I

E

E

L

I

R

S

A

R

I

L

Q

H

K

K

R

R

L

L

G

G

I

R

T

T

T

M

I

I

L

Y

V

V

T

T

H

H

D

D

Q

Q

V

V

E

E

A

A

M

M

T

T

M

L

S

A

D

D

R

K

I

I

A

V

V

V

M

L

H

D

R

A

G

G

V

R

I

V

A

A

Q

Q

L

V

S

G

T

K

P

P

Y

L

D

E

A

L

Y

Y

E

H

R

Y

P

P

A

A

T

D

P
x
R

F

F

A

V

S

A

T

G

F

F

L

I

G

G

-

S

-

P

R

K

T

M

N

N

A
x
F

F

L

S

P

G

V

E

K

V

V

L

T

R

A

R

T

N

A

P

I

R

D

C

Q

A

V

E

Q

V

V

D

E

V

L

A

P

G

M

T

P

T

N

-

R

-

Q

L

V

H
x
W

V

L

P

P

H

V

E

E

G

S

R

R

H

D

V

V

D

Q

G

V

T
x
G

V

A

N

N

V

M

Y
x
S

-

L

-
x
G

I

I

R

R

P

P

E

E

K

H

V

L

H

L

L

P

A

S

N

D

-

I

G

A

D

D

A

V

R

I

L

L

R

E

G
|
G

R

E

I

V

A

Q

T

V

R

V

V
x
E

F

Q

V

L

G

G

N

N

Q

E

A85 (= A85) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ R106) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (= V114) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ A117) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ S119) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ K124) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G137) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D158) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (≠ P228) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
F241 (≠ A239) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
W267 (≠ H262) mutation to G: Normal maltose transport but constitutive mal gene expression.
G278 (≠ T273) mutation to P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
S282 (≠ Y277) mutation to L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G284 (vs. gap) mutation to S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G302 (= G294) mutation to D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
E308 (≠ V300) mutation to Q: Maltose transport is affected but retains ability to interact with MalT.

Sites not aligning to the query:

322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
41% identity, 81% coverage: 1:289/359 of query aligns to 1:292/369 of P19566

query
sites
P19566

M

M

A

A

F

S

L

V

T

Q

L

L

Q

R

G

N

I

V

S

T

K

K

R

A

Y

W

G

G

D

D

F

V

T

V

A

V

I

S

E

K

Q

D

L

I

D

N

L

L

A

D

V

I

E

H

R

D

G

G

E

E

L

F

L

V

S

V

L

F

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

S

T

T

T

L

L

L

Q

R

M

M

V

I

A

A

G

G

F

L

I

E

T

T

P

I

T

T

T

S

G

G

R

D

I

L

L

F

L

I

D

G

G

E

R

T

D

R

I

M

T

N

N

D

E

I

R

P

P

P

E

A

K

E

R

R

G

G

I

V

G

G

V

M

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L
|
L

F

Y

P

P

H

H

M

L

T

S

V

V

A

A

G

E

N

N

V

M

G

S

F

F

G

G

L

L

E

K

M

L

R

A

K

G

V

A

R

K

K

E

E

V

R

M

D

N

E

Q

R

R

V

V

A

N

E

Q

A

V

L

A

S

E

L

V

V

L

R

Q

L

L

K

A

G

H

L

L

G

L

H

E

R

R

Y

K

P

P

K

K

E

A

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

I

L

A

V

M

A

Q

E

P

P

E

R

L

V

L

F

L

L

D

D

E

E

P
|
P

M

L

S

S

N

N

L

L

D
|
D

A

A

K

A

L

L

R

R

E

V

E

Q

M

M

H

R

I

I

E

E

L

I

R

S

A

R

I

L

Q

H

K

K

R

R

L

L

G

G

I

R

T

T

T

M

I

I

L

Y

V

V

T

T

H

H

D

D

Q

Q

V

V

E

E

A

A

M

M

T

T

M

L

S

A

D

D

R

K

I

I

A

V

V

V

M

L

H

D

R

A

G

G

V

R

I

V

A

A

Q

Q

L

V

S

G

T

K

P

P

Y

L

D

E

A

L

Y

Y

E

H

R

Y

P

P

A

A

T

D

P

R

F

F

A

V

S

A

T

G

F

F

L

I

G

G

-

S

-

P

R

K

T

M

N

N

A

F

F

L

S

P

G

V

E

K

V

V

L

T

R

A

R

T

N

A

P

I

R

E

C

Q

A

V

E

Q

V

V

D

E

V

L

A

P

G

N

-

R

-

Q

T

I

T

W

L

L

H

P

V

V

P

E

H

S

E

R

G

G

R

V

H

Q

V

V

D

G

G

A

T

N

V

M

N

S

V

L

Y

G

I

I

R

R

P

P

E

E

K

-

V

-

H

H

L

L

A

L

N

P

G

S

D

D

L86 (= L86) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P160) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D165) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

2awoA Crystal structure of the adp-mg-bound e. Coli malk (crystallized with adp-mg) (see paper)
39% identity, 85% coverage: 2:305/359 of query aligns to 1:312/372 of 2awoA

query
sites
2awoA

A

A

F

S

L

V

T

Q

L

L

Q

Q

G

N

I

V

S

T

K

K

R

A

Y
x
W

G

G

D

E

F

V

T

V

A
x
V

I

S

E

K

Q

D

L

I

D

N

L

L

A

D

V

I

E

H

R

E

G

G

E

E

L

F

L

V

S

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

L

L

L

Q

R

M

M

V

I

A

A

G

G

F

L

I

E

T

T

P

I

T

T

T

S

G

G

R

D

I

L

L

F

L

I

D

G

G

E

R

K

D

R

I

M

T

N

N

D

E

T

R

P

P

P

E

A

K

E

R

R

G

G

I

V

G

G

V

M

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

A

A

G

E

N

N

V

M

G

S

F

F

G

G

L

L

E

K

M

L

R

A

K

G

V

A

R

K

K

E

E

V

R

I

D

N

E

Q

R

R

V

V

A

N

E

Q

A

V

L

A

S

E

L

V

V

L

R

Q

L

L

K

A

G

H

L

L

G

L

H

D

R

R

Y

K

P

P

K

K

E

A

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

I

L

A

V

M

A

Q

E

P

P

E

S

L

V

L

F

L

L

D
|
D

E

E

P

P

M

L

S

S

N

N

L

L

D

D

A

A

K

A

L

L

R

R

E

V

E

Q

M

M

H

R

I

I

E

E

L

I

R

S

A

R

I

L

Q

H

K

K

R

R

L

L

G

G

I

R

T

T

T

M

I

I

L

Y

V

V

T

T

H

H

D

D

Q

Q

V

V

E

E

A

A

M

M

T

T

M

L

S

A

D

D

R

K

I

I

A

V

V

V

M

L

H

D

R

A

G

G

V

R

I

V

A

A

Q

Q

L

V

S

G

T

K

P

P

Y

L

D

E

A

L

Y

Y

E

H

R

Y

P

P

A

A

T

D

P

R

F

F

A

V

S

A

T

G

F

F

L

I

G

G

-

S

-

P

R

K

T

M

N

N

A

F

F

L

S

P

G

V

E

K

V

V

L

T

R

A

R

T

N

A

P

I

R

D

C

Q

A

V

E

Q

V

V

D

E

V

L

A

P

G

M

T

P

T

N

-

R

-

Q

L

V

H

W

V

L

P

P

H

V

E

E

G

S

R

R

H

D

V

V

D

Q

G

V

T

G

V

A

N

N

V

M

Y

S

-

L

-

G

I

I

R

R

P

P

E

E

K

H

V

L

H

L

L

P

A

S

N

D

-

I

G

A

D

D

A

V

R

I

L

L

R

E

G

G

R

E

I

V

A

Q

T

V

R

V

V

E

F

Q

V

L

G

G

N

N

Q

E

binding adenosine-5'-diphosphate: W12 (≠ Y13), V17 (≠ A18), S37 (= S38), G38 (= G39), C39 (= C40), G40 (= G41), K41 (= K42), S42 (≠ T43), T43 (= T44)
binding magnesium ion: S42 (≠ T43), D157 (= D158)

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
39% identity, 85% coverage: 2:305/359 of query aligns to 1:312/374 of 2awnB

query
sites
2awnB

A

A

F

S

L

V

T

Q

L

L

Q

Q

G

N

I

V

S

T

K

K

R

A

Y
x
W

G

G

D

E

F

V

T

V

A
x
V

I

S

E

K

Q

D

L

I

D

N

L

L

A

D

V

I

E

H

R

E

G

G

E

E

L

F

L

V

S

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

L

L

L

Q

R

M

M

V

I

A

A

G

G

F

L

I

E

T

T

P

I

T

T

T

S

G

G

R

D

I

L

L

F

L

I

D

G

G

E

R

K

D

R

I

M

T

N

N

D

E

T

R

P

P

P

E

A

K

E

R

R

G

G

I

V

G

G

V

M

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

A

A

G

E

N

N

V

M

G

S

F

F

G

G

L

L

E

K

M

L

R

A

K

G

V

A

R

K

K

E

E

V

R

I

D

N

E

Q

R

R

V

V

A

N

E

Q

A

V

L

A

S

E

L

V

V

L

R

Q

L

L

K

A

G

H

L

L

G

L

H

D

R

R

Y

K

P

P

K

K

E

A

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

I

L

A

V

M

A

Q

E

P

P

E

S

L

V

L

F

L

L

D

D

E

E

P

P

M

L

S

S

N

N

L

L

D

D

A

A

K

A

L

L

R

R

E

V

E

Q

M

M

H

R

I

I

E

E

L

I

R

S

A

R

I

L

Q

H

K

K

R

R

L

L

G

G

I

R

T

T

T

M

I

I

L

Y

V

V

T

T

H

H

D

D

Q

Q

V

V

E

E

A

A

M

M

T

T

M

L

S

A

D

D

R

K

I

I

A

V

V

V

M

L

H

D

R

A

G

G

V

R

I

V

A

A

Q

Q

L

V

S

G

T

K

P

P

Y

L

D

E

A

L

Y

Y

E

H

R

Y

P

P

A

A

T

D

P

R

F

F

A

V

S

A

T

G

F

F

L

I

G

G

-

S

-

P

R

K

T

M

N

N

A

F

F

L

S

P

G

V

E

K

V

V

L

T

R

A

R

T

N

A

P

I

R

D

C

Q

A

V

E

Q

V

V

D

E

V

L

A

P

G

M

T

P

T

N

-

R

-

Q

L

V

H

W

V

L

P

P

H

V

E

E

G

S

R

R

H

D

V

V

D

Q

G

V

T

G

V

A

N

N

V

M

Y

S

-

L

-

G

I

I

R

R

P

P

E

E

K

H

V

L

H

L

L

P

A

S

N

D

-

I

G

A

D

D

A

V

R

I

L

L

R

E

G

G

R

E

I

V

A

Q

T

V

R

V

V

E

F

Q

V

L

G

G

N

N

Q

E

binding adenosine-5'-diphosphate: W12 (≠ Y13), V17 (≠ A18), S37 (= S38), G38 (= G39), C39 (= C40), G40 (= G41), K41 (= K42), S42 (≠ T43), T43 (= T44)

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
39% identity, 85% coverage: 2:305/359 of query aligns to 1:312/371 of 3puyA

query
sites
3puyA

A

A

F

S

L

V

T

Q

L

L

Q

Q

G

N

I

V

S

T

K

K

R

A

Y
x
W

G

G

D

E

F

V

T

V

A
x
V

I

S

E

K

Q

D

L

I

D

N

L

L

A

D

V

I

E

H

R

E

G

G

E

E

L

F

L

V

S

V

L

F

L

V

G

G

P
|
P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

L

L

L

Q

R

M

M

V

I

A

A

G

G

F

L

I

E

T

T

P

I

T

T

T

S

G

G

R

D

I

L

L

F

L

I

D

G

G

E

R

K

D

R

I

M

T

N

N

D

E

T

R

P

P

P

E

A

K

E

R

R

G

G

I

V

G

G

V

M

V

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

A

A

G

E

N

N

V

M

G

S

F

F

G

G

L

L

E

K

M

L

R

A

K

G

V

A

R

K

K

E

E

V

R

I

D

N

E

Q

R

R

V

V

A

N

E

Q

A

V

L

A

S

E

L

V

V

L

R

Q

L

L

K

A

G

H

L

L

G

L

H

D

R
|
R

Y

K

P

P

K

K

E
x
A

L

L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

I

L

A

V

M

A

Q

E

P

P

E

S

L

V

L

F

L

L

D
|
D

E
|
E

P

P

M

L

S

S

N

N

L

L

D

D

A

A

K

A

L

L

R

R

E

V

E

Q

M

M

H

R

I

I

E

E

L

I

R

S

A

R

I

L

Q

H

K

K

R

R

L

L

G

G

I

R

T

T

T

M

I

I

L

Y

V

V

T

T

H
|
H

D

D

Q

Q

V

V

E

E

A

A

M

M

T

T

M

L

S

A

D

D

R

K

I

I

A

V

V

V

M

L

H

D

R

A

G

G

V

R

I

V

A

A

Q

Q

L

V

S

G

T

K

P

P

Y

L

D

E

A

L

Y

Y

E

H

R

Y

P

P

A

A

T

D

P

R

F

F

A

V

S

A

T

G

F

F

L

I

G

G

-

S

-

P

R

K

T

M

N

N

A

F

F

L

S

P

G

V

E

K

V

V

L

T

R

A

R

T

N

A

P

I

R

D

C

Q

A

V

E

Q

V

V

D

E

V

L

A

P

G

M

T

P

T

N

-

R

-

Q

L

V

H

W

V

L

P

P

H

V

E

E

G

S

R

R

H

D

V

V

D

Q

G

V

T

G

V

A

N

N

V

M

Y

S

-

L

-

G

I

I

R

R

P

P

E

E

K

H

V

L

H

L

L

P

A

S

N

D

-

I

G

A

D

D

A

V

R

I

L

L

R

E

G

G

R

E

I

V

A

Q

T

V

R

V

V

E

F

Q

V

L

G

G

N

N

Q

E

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y13), V17 (≠ A18), P36 (= P37), S37 (= S38), G38 (= G39), C39 (= C40), G40 (= G41), K41 (= K42), S42 (≠ T43), T43 (= T44), Q81 (= Q82), R128 (= R129), A132 (≠ E133), S134 (= S135), G135 (= G136), G136 (= G137), Q137 (= Q138), E158 (= E159), H191 (= H192)
binding magnesium ion: S42 (≠ T43), Q81 (= Q82), D157 (= D158)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
39% identity, 85% coverage: 2:305/359 of query aligns to 1:312/371 of 3puxA

query
sites
3puxA

A

A

F

S

L

V

T

Q

L

L

Q

Q

G

N

I

V

S

T

K

K

R

A

Y
x
W

G

G

D

E

F

V

T

V

A
x
V

I

S

E

K

Q

D

L

I

D

N

L

L

A

D

V

I

E

H

R

E

G

G

E

E

L

F

L

V

S

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

L

L

L

Q

R

M

M

V

I

A

A

G

G

F

L

I

E

T

T

P

I

T

T

T

S

G

G

R

D

I

L

L

F

L

I

D

G

G

E

R

K

D

R

I

M

T

N

N

D

E

T

R

P

P

P

E

A

K

E

R

R

G

G

I

V

G

G

V

M

V

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

A

A

G

E

N

N

V

M

G

S

F

F

G

G

L

L

E

K

M

L

R

A

K

G

V

A

R

K

K

E

E

V

R

I

D

N

E

Q

R

R

V

V

A

N

E

Q

A

V

L

A

S

E

L

V

V

L

R

Q

L

L

K

A

G

H

L

L

G

L

H

D

R
|
R

Y

K

P

P

K

K

E
x
A

L
|
L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

I

L

A

V

M

A

Q

E

P

P

E

S

L

V

L

F

L

L

D
|
D

E
|
E

P

P

M

L

S

S

N

N

L

L

D

D

A

A

K

A

L

L

R

R

E

V

E

Q

M

M

H

R

I

I

E

E

L

I

R

S

A

R

I

L

Q

H

K

K

R

R

L

L

G

G

I

R

T

T

T

M

I

I

L

Y

V

V

T

T

H
|
H

D

D

Q

Q

V

V

E

E

A

A

M

M

T

T

M

L

S

A

D

D

R

K

I

I

A

V

V

V

M

L

H

D

R

A

G

G

V

R

I

V

A

A

Q

Q

L

V

S

G

T

K

P

P

Y

L

D

E

A

L

Y

Y

E

H

R

Y

P

P

A

A

T

D

P

R

F

F

A

V

S

A

T

G

F

F

L

I

G

G

-

S

-

P

R

K

T

M

N

N

A

F

F

L

S

P

G

V

E

K

V

V

L

T

R

A

R

T

N

A

P

I

R

D

C

Q

A

V

E

Q

V

V

D

E

V

L

A

P

G

M

T

P

T

N

-

R

-

Q

L

V

H

W

V

L

P

P

H

V

E

E

G

S

R

R

H

D

V

V

D

Q

G

V

T

G

V

A

N

N

V

M

Y

S

-

L

-

G

I

I

R

R

P

P

E

E

K

H

V

L

H

L

L

P

A

S

N

D

-

I

G

A

D

D

A

V

R

I

L

L

R

E

G

G

R

E

I

V

A

Q

T

V

R

V

V

E

F

Q

V

L

G

G

N

N

Q

E

binding adenosine-5'-diphosphate: W12 (≠ Y13), V17 (≠ A18), S37 (= S38), G38 (= G39), C39 (= C40), G40 (= G41), K41 (= K42), S42 (≠ T43), T43 (= T44), R128 (= R129), A132 (≠ E133), L133 (= L134), S134 (= S135), Q137 (= Q138)
binding beryllium trifluoride ion: S37 (= S38), G38 (= G39), K41 (= K42), Q81 (= Q82), S134 (= S135), G135 (= G136), G136 (= G137), E158 (= E159), H191 (= H192)
binding magnesium ion: S42 (≠ T43), Q81 (= Q82), D157 (= D158)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
39% identity, 85% coverage: 2:305/359 of query aligns to 1:312/371 of 3puwA

query
sites
3puwA

A

A

F

S

L

V

T

Q

L

L

Q

Q

G

N

I

V

S

T

K

K

R

A

Y
x
W

G

G

D

E

F

V

T

V

A
x
V

I

S

E

K

Q

D

L

I

D

N

L

L

A

D

V

I

E

H

R

E

G

G

E

E

L

F

L

V

S

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

L

L

L

Q

R

M

M

V

I

A

A

G

G

F

L

I

E

T

T

P

I

T

T

T

S

G

G

R

D

I

L

L

F

L

I

D

G

G

E

R

K

D

R

I

M

T

N

N

D

E

T

R

P

P

P

E

A

K

E

R

R

G

G

I

V

G

G

V

M

V

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

A

A

G

E

N

N

V

M

G

S

F

F

G

G

L

L

E

K

M

L

R

A

K

G

V

A

R

K

K

E

E

V

R

I

D

N

E

Q

R

R

V

V

A

N

E

Q

A

V

L

A

S

E

L

V

V

L

R

Q

L

L

K

A

G

H

L

L

G

L

H

D

R
|
R

Y

K

P

P

K

K

E
x
A

L

L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

I

L

A

V

M

A

Q

E

P

P

E

S

L

V

L

F

L

L

D

D

E
|
E

P

P

M

L

S

S

N
|
N

L

L

D

D

A

A

K

A

L

L

R

R

E

V

E

Q

M

M

H

R

I

I

E

E

L

I

R

S

A

R

I

L

Q

H

K

K

R

R

L

L

G

G

I

R

T

T

T

M

I

I

L

Y

V

V

T

T

H
|
H

D

D

Q

Q

V

V

E

E

A

A

M

M

T

T

M

L

S

A

D

D

R

K

I

I

A

V

V

V

M

L

H

D

R

A

G

G

V

R

I

V

A

A

Q

Q

L

V

S

G

T

K

P

P

Y

L

D

E

A

L

Y

Y

E

H

R

Y

P

P

A

A

T

D

P

R

F

F

A

V

S

A

T

G

F

F

L

I

G

G

-

S

-

P

R

K

T

M

N

N

A

F

F

L

S

P

G

V

E

K

V

V

L

T

R

A

R

T

N

A

P

I

R

D

C

Q

A

V

E

Q

V

V

D

E

V

L

A

P

G

M

T

P

T

N

-

R

-

Q

L

V

H

W

V

L

P

P

H

V

E

E

G

S

R

R

H

D

V

V

D

Q

G

V

T

G

V

A

N

N

V

M

Y

S

-

L

-

G

I

I

R

R

P

P

E

E

K

H

V

L

H

L

L

P

A

S

N

D

-

I

G

A

D

D

A

V

R

I

L

L

R

E

G

G

R

E

I

V

A

Q

T

V

R

V

V

E

F

Q

V

L

G

G

N

N

Q

E

binding adenosine-5'-diphosphate: W12 (≠ Y13), V17 (≠ A18), S37 (= S38), G38 (= G39), C39 (= C40), G40 (= G41), K41 (= K42), S42 (≠ T43), T43 (= T44), R128 (= R129), A132 (≠ E133), S134 (= S135), Q137 (= Q138)
binding tetrafluoroaluminate ion: S37 (= S38), G38 (= G39), K41 (= K42), Q81 (= Q82), S134 (= S135), G135 (= G136), G136 (= G137), E158 (= E159), N162 (= N163), H191 (= H192)
binding magnesium ion: S42 (≠ T43), Q81 (= Q82)

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
39% identity, 84% coverage: 4:305/359 of query aligns to 1:310/367 of 1q12A

query
sites
1q12A

L

V

T

Q

L

L

Q

Q

G

N

I

V

S

T

K

K

R

A

Y
x
W

G

G

D

E

F

V

T

V

A
x
V

I

S

E

K

Q

D

L

I

D

N

L

L

A

D

V

I

E

H

R

E

G

G

E

E

L

F

L

V

S

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

L

L

L

Q

R

M

M

V

I

A

A

G

G

F

L

I

E

T

T

P

I

T

T

T

S

G

G

R

D

I

L

L

F

L

I

D

G

G

E

R

K

D

R

I

M

T

N

N

D

E

T

R

P

P

P

E

A

K

E

R

R

G

G

I

V

G

G

V

M

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

A

A

G

E

N

N

V

M

G

S

F

F

G

G

L

L

E

K

M

L

R

A

K

G

V

A

R

K

K

E

E

V

R

I

D

N

E

Q

R

R

V

V

A

N

E

Q

A

V

L

A

S

E

L

V

V

L

R

Q

L

L

K

A

G

H

L

L

G

L

H

D

R
|
R

Y

K

P

P

K
|
K

E
x
A

L
|
L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

I

L

A

V

M

A

Q

E

P

P

E

S

L

V

L

F

L

L

D

D

E

E

P

P

M

L

S

S

N
|
N

L

L

D

D

A

A

K

A

L

L

R

R

E

V

E

Q

M

M

H

R

I

I

E

E

L

I

R

S

A

R

I

L

Q

H

K

K

R

R

L

L

G

G

I

R

T

T

T

M

I

I

L

Y

V

V

T

T

H
|
H

D

D

Q

Q

V

V

E

E

A

A

M

M

T

T

M

L

S

A

D

D

R

K

I

I

A

V

V

V

M

L

H

D

R

A

G

G

V

R

I

V

A

A

Q

Q

L

V

S

G

T

K

P

P

Y

L

D

E

A

L

Y

Y

E

H

R

Y

P

P

A

A

T

D

P

R

F

F

A

V

S

A

T

G

F

F

L

I

G

G

-

S

-

P

R

K

T

M

N

N

A

F

F

L

S

P

G

V

E

K

V

V

L

T

R

A

R

T

N

A

P

I

R

D

C

Q

A

V

E

Q

V

V

D

E

V

L

A

P

G

M

T

P

T

N

-

R

-

Q

L

V

H

W

V

L

P

P

H

V

E

E

G

S

R

R

H

D

V

V

D

Q

G

V

T

G

V

A

N

N

V

M

Y

S

-

L

-

G

I

I

R

R

P

P

E

E

K

H

V

L

H

L

L

P

A

S

N

D

-

I

G

A

D

D

A

V

R

I

L

L

R

E

G

G

R

E

I

V

A

Q

T

V

R

V

V

E

F

Q

V

L

G

G

N

N

Q

E

binding adenosine-5'-triphosphate: W10 (≠ Y13), V15 (≠ A18), S35 (= S38), G36 (= G39), C37 (= C40), G38 (= G41), K39 (= K42), S40 (≠ T43), T41 (= T44), R126 (= R129), K129 (= K132), A130 (≠ E133), L131 (= L134), S132 (= S135), G133 (= G136), G134 (= G137), Q135 (= Q138), N160 (= N163), H189 (= H192)

7cagC Mycobacterium smegmatis lpqy-sugabc complex in the catalytic intermediate state (see paper)
41% identity, 82% coverage: 2:294/359 of query aligns to 1:297/362 of 7cagC

query
sites
7cagC

A

A

F

E

L

I

T

V

L

L

Q

D

G

R

I

V

S

T

K

K

R

S

Y

Y

G

P

D

-

F

-

T
x
A

A

A

I

V

E

K

Q

E

L

F

D

S

L

M

A

T

V

I

E

A

R

D

G

G

E

E

L

F

L

I

S

I

L

L

L

V

G

G

P
|
P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

T

L

L

Q

N

M

M

V

I

A

A

G

G

F

L

I

E

T

E

P

I

T

T

T

S

G

G

R

E

I

L

L

R

L

I

D

G

G

G

R

E

D

R

I

V

T

N

N

E

E

K

R

A

P

P

E

K

K

D

R

R

G

D

I

I

G

A

V

M

V

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

T

T

V

V

A

R

G

Q

N

N

V

I

G

A

F

F

G

P

L

L

E

T

M

L

R

A

K

K

V

V

R

P

R

K

K

A

E

E

R

I

D

A

E

A

R

K

V

V

A

E

E

E

A

T

L

A

S

K

L

I

V

L

R

D

L

L

K

S

G

E

L
|
L

G

L

H

D

R
|
R

Y

K

P

P

K

G

E
x
Q

L

L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

I

M

A

G

R

R

A

A

I

I

A

V

M

R

Q

S

P

P

E

K

L

A

L

F

L

L

L

M

D

D

E

Q

P

P

M

L

S

S

N

N

L

L

D

D

A

A

K

K

L

L

R

R

E

V

E

Q

M

M

H

R

I

A

E

E

L

I

R

S

A

R

I

L

Q

Q

K

D

R

R

L

L

G

G

I

T

T

T

I

V

L

Y

V

V

T

T

H

H

D

D

Q

Q

V

T

E

E

A

A

M

M

T

T

M

L

S

G

D

D

R

R

I

V

A

V

V

V

M

M

H

L

R

A

G

G

V

E

I

V

A

Q

Q

Q

L

I

S

G

T

T

P

P

Y

D

D

E

A

L

Y

Y

E

S

R

S

P

P

A

A

T

N

P

L

F

F

A

V

S

A

T

G

F

F

L

I

G

G

R

S

-

P

-

A

T

M

N

N

A

F

F

F

S

P

G

A

E

T

V

-

L

-

R

-

R

R

N

T

P

D

R

V

C

G

A

V

E

R

V

L

D

P

V

F

A

G

G

E

T

V

T

T

L

L

H

-

V

T

P

P

H

H

E

M

G

L

R

D

H

L

V

L

D

D

G

K

-

Q

-

A

-

R

-

P

-

E

T

N

V

I

N

I

V

V

Y

G

I

I

R

R

P

P

E

E

K

H

V

I

H

E

-

D

-

S

-

A

L

L

A

L

N

D

G

G

D

Y

A

A

R

R

L

I

R

R

G

A

binding adenosine-5'-triphosphate: A14 (≠ T17), P34 (= P37), S35 (= S38), G36 (= G39), C37 (= C40), G38 (= G41), K39 (= K42), S40 (≠ T43), T41 (= T44), Q79 (= Q82), L123 (= L126), R126 (= R129), Q130 (≠ E133), S132 (= S135), G133 (= G136), G134 (= G137), Q135 (= Q138)
binding magnesium ion: S40 (≠ T43), Q79 (= Q82)

3d31A Modbc from methanosarcina acetivorans (see paper)
36% identity, 85% coverage: 3:308/359 of query aligns to 1:301/348 of 3d31A

query
sites
3d31A

F

M

L

I

T

E

L

I

Q

E

G

S

I

L

S

S

K

R

R

K

Y

W

G

K

D

N

F

F

T

S

A

-

I

L

E

D

Q

N

L

L

D

S

L

L

A

K

V

V

E

E

R

S

G

G

E

E

L

Y

L

F

S

V

L

I

L

L

G

G

P

P

S

T

G

G

C

A

G

G

K

K

T

T

T

L

T

F

L

L

Q

E

M

L

V

I

A

A

G

G

F

F

I

H

T

V

P

P

T

D

T

S

G

G

R

R

I

I

L

L

D

D

G

G

R

K

D

D

I

V

T

T

N

D

E

L

R

S

P

P

E

E

K

K

R

H

G

D

I

I

G

A

V

F

V

V

F

Y

Q

Q

S

N

Y

Y

A

S

L

L

F

F

P

P

H

H

M

M

T

N

V

V

A

K

G

K

N

N

V

L

G

E

F

F

G

G

L

M

E

R

M

M

R

K

K

K

V

I

R

K

R

D

K

P

E

-

R

-

D

-

E

K

R

R

V

V

A

L

E

D

A

T

L

A

S

R

L

D

V

L

R

K

L

I

K

E

G

H

L

L

G

L

H

D

R

R

Y

N

P

P

K

L

E

T

L

L

S

S

G

G

Q

E

R

Q

Q

Q

R

R

V

V

A

A

I

L

A

A

R

R

A

A

I

L

A

V

M

T

Q

N

P

P

E

K

L

I

L

L

D

D

E

E

P

P

M

L

S

S

N

A

L

L

D

D

A

P

K

R

L

T

R

Q

E

E

E

N

M

A

H

R

I

E

E

M

L

L

R

S

A

V

I

L

Q

H

K

K

R

K

L

N

G

K

I

L

T

T

T

V

I

L

L

H

V

I

T

T

H

H

D

D

Q

Q

V

T

E

E

A

A

M

R

T

I

M

M

S

A

D

D

R

R

I

I

A

A

V

V

M

V

H

M

R

D

G

G

V

K

I

L

A

I

Q

Q

L

V

S

G

T

K

P

P

Y

E

D

E

A

I

Y

F

E

E

R

K

P

P

A

V

T

E

P

G

F

R

A

V

S

A

T

S

F

F

L

V

G

G