SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS10075 FitnessBrowser__BFirm:BPHYT_RS10075 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 14 hits to proteins with known functional sites (download)

Q47945 Alcohol dehydrogenase (quinone), cytochrome c subunit; ADH; Alcohol dehydrogenase (quinone), subunit II; Cytochrome c-553; Cytochrome c553; Ethanol:Q2 reductase; G3-ADH subunit II; Quinohemoprotein-cytochrome c complex; Ubiquinol oxidase; EC 1.1.5.5 from Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans) (see paper)
42% identity, 98% coverage: 3:428/433 of query aligns to 24:434/478 of Q47945

query
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Q47945

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Q37 (≠ T21) modified: Pyrrolidone carboxylic acid

Sites not aligning to the query:

1:36 signal peptide

8gy2B Alcohol dehydrogenase (quinone), cytochrome c subunit
43% identity, 94% coverage: 23:428/433 of query aligns to 1:396/433 of 8gy2B

query
sites
8gy2B

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binding heme c: S16 (≠ A38), D17 (= D39), C18 (= C40), C21 (= C43), H22 (= H44), I43 (= I76), Y44 (≠ N77), T46 (≠ S79), I48 (= I81), Y59 (= Y92), F64 (= F97), L68 (= L101), R73 (≠ A106), V79 (≠ L112), Y80 (= Y113), A82 (= A115), M83 (= M116), P84 (= P117), F88 (= F121), M99 (≠ L132), F103 (= F136), R126 (= R159), H165 (= H197), H165 (= H197), C166 (= C198), C169 (= C201), H170 (= H202), I201 (≠ F231), A202 (= A232), P203 (= P233), L205 (= L235), L213 (= L243), W216 (≠ S246), F224 (= F254), L225 (= L255), A234 (≠ V264), V235 (≠ T265), V235 (≠ T265), F236 (= F266), F236 (= F266), M239 (= M269), V242 (= V272), N301 (≠ Y334), C302 (= C335), C305 (= C338), H306 (= H339), M316 (≠ K349), F317 (≠ Y350), P318 (= P351), L320 (= L353), N323 (= N356), P324 (= P357), V325 (≠ A358), V337 (≠ L370), G342 (= G375), L344 (≠ S377), P351 (= P384), S352 (≠ E385), A353 (≠ P386), V354 (≠ R387), A355 (≠ K388), M356 (= M389), F359 (= F392), M375 (≠ I408)
binding ubiquinone-10: A20 (≠ G42), C21 (= C43), L34 (= L67), S38 (= S71), P39 (= P72), Y80 (= Y113), P81 (= P114), A82 (= A115), L129 (= L162), W132 (= W165), E168 (≠ A200), R173 (= R205), M177 (≠ Y209), I197 (≠ N227), D241 (≠ Q271)

Sites not aligning to the query:

binding heme c: 428

7w2jC Cryo-em structure of membrane-bound fructose dehydrogenase from gluconobacter japonicus
39% identity, 93% coverage: 28:428/433 of query aligns to 1:386/418 of 7w2jC

query
sites
7w2jC

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S

C
|
C

H
|
H

Q

Q

S

S

D

D

G

G

A

K

G

G

V

S

P

K

Q

D

-

G

K
x
Y

Y
|
Y

P
|
P

R

S

L
|
L

A

V

G

G

N
|
N

P
x
T

A
x
T

V

T

L

G

A
x
Q

P

L

Q

N

T

P

T

N

S
x
D

L
|
L

V

I

R

A

L
x
S

L
x
I

V

L

E

Y

G

G

G
x
V

G

D

S
x
R

P

-

N

T

T

T

D

D

G

N

G

-

P

-

E

H

P

E

R
x
I

K

L

M
|
M

P
|
P

A

A

F
|
F

-

G

-

P

-

D

-

S

-

L

A

V

D

Q

K

P

L

L

T

T

D

D

S

E

E

Q

L

I

A

A

H

T

V

I

L

A

T

D

F

Y

I

V

R

L

N

S

T

H

W

F

G

G

N

N

A

A

A

Q

E

A

P

T

V

V

T

S

T

A

R

D

D

A

V

V

S

K

A

Q

V

V

R

R

binding heme c: C13 (= C40), C16 (= C43), H17 (= H44), I39 (= I76), S41 (= S78), T42 (≠ S79), N43 (= N80), I44 (= I81), I52 (= I89), Y55 (= Y92), F60 (= F97), A63 (= A100), L75 (= L112), Y76 (= Y113), A78 (= A115), M79 (= M116), P80 (= P117), Y84 (≠ F121), R122 (= R159), H161 (= H197), C162 (= C198), C165 (= C201), H166 (= H202), W189 (= W230), R190 (≠ F231), A191 (= A232), P192 (= P233), I194 (≠ L235), W205 (≠ S246), Y213 (≠ F254), L214 (= L255), R223 (≠ V264), P227 (≠ S268), M228 (= M269), L236 (≠ T277), V289 (≠ Y334), C290 (= C335), C293 (= C338), H294 (= H339), Y305 (≠ K349), Y306 (= Y350), P307 (= P351), L309 (= L353), N312 (= N356), T313 (≠ P357), T314 (≠ A358), T314 (≠ A358), Q317 (≠ A361), D322 (≠ S366), L323 (= L367), S326 (≠ L370), I327 (≠ L371), V331 (≠ G375), R333 (≠ S377), I340 (≠ R387), M342 (= M389), P343 (= P390), F345 (= F392)

8gy3A Large subunit of aldehyde dehydrogenase
34% identity, 94% coverage: 3:411/433 of query aligns to 14:410/440 of 8gy3A

query
sites
8gy3A

L

I

G

G

A

F

L

L

A

A

S

Y

S

A

-

W

Y

Y

T

P

A

A

A

I

S

A

A

P

A

I

P

P

G

R

S

P

A

A

T

A

S

S

A

S

D

F

T

S

A

A

Q

D

-

A

I

I

A

S

R

R

G

G

A

E

Y

I

L

V

A

A

K

N

A

G

D
x
Y

C
|
C

A

A

G
x
E

C
|
C

H
|
H

T

T

A

-

P

-

R

-

V

-

R

R

P

V

G

D

T

G

A

K

P

P

I

G

P

P

A

E

A

-

P

-

F

L

A

A

G

G

G

D

L
x
F

G

K

M

M

A

A

S
x
T

P

P

F
|
F

G

G

T

D

I
|
I

N

F

S
|
S

N

N

I
|
I

T

T

P

P

D

D

P

E

Q

E

A

W

G

G

I

I

G

G

L

N

Y
x
W

S

S

Y

L

D

A

F
|
F

A

K

H

R

A

A

L
x
M

R

N

D

K

G

G

V

I

A

A

R

R

G

D

G

G

K

S

R

Q

L
|
L

Y
|
Y

P
|
P

A
|
A

M
x
F

P
|
P

Y

F

P

D

S

H

F
|
F

A

T

K

K

I

V

D

S

D

D

D

Q

D

D

M

V

H

S

A

D

L
|
L

Y

Y

A

A

Y

Y

F

L

M

M

H

-

G

-

V

T

R

R

P

P

V

A

A

V

T

H

P

L

A

K

P

P

-

R

P

D

T

N

R

T

L

V

P

P

F

F

P

P

F
x
I

N

N

Q

I

R
|
R

-

L

-
x
I

-
x
G

-
x
Q

-

G

-
x
F

W
|
W

A

K

L

L

M

L

F

F

W

-

D

-

W

-

A

-

F

F

V

T

P

P

G

G

E

-

R

R

F

Y

K

Q

P

N

E

D

A

P

G

K

R

H

D

D

A

A

Q

Q

W

W

N

N

R

R

G

G

A

A

Y

Y

L

L

V

A

Q

E

S

G

L

N

G

E

H
|
H

C
|
C

G

G

A
|
A

C
|
C

H
|
H

T

T

P

P

R
|
R

G

-

P

-

A

-

Y

-

E

N

E

L

R
x
L

G

G

Y

A

T

E

E

K

A

M

S

S

P

S

L

V

Y

Y

L

-

T

D

G

G

G

A

T

V

N

I

D
|
D

H

G

W
|
W

F
x
I

A
|
A

P
|
P

N

P

L
|
L

T

N

A

-

D

D

T

H

G
x
N

S

P

G

T

L

P

G

V

R

V

S
x
W

S

T

A

E

E

D

D

E

I

L

A

F

A

Q

F
x
Y

L
|
L

K

R

H

F

G

G

-

V

-

A

-

P

-

L

H

H

G
|
G

G
x
S

G

A

L
x
A

V

-

T

-

F

-

G

G

S
x
P

M
|
M

V

S

Q

P

V

V

V

P

E

H

D

R

S

F

T

L

Q

S

Y

K

L

I

D

P

D

E

A

E

D

D

L

V

A

H

A

A

I

I

A

A

T

H

Y

Y

L
x
Y

K

A

S

D

L

V

P

D

P

K

R

A

A

A

Q

Q

-

R

-

S

S

S

G

G

H

D

F

Q

N

A

A

A

G

I

S

T

R

R

G

A

A

M

L

Q

Q

M

S

S

V

G

E

R

A

D

L

L

K

T

T

G

G

P

E

Q

L

P

E

L

R

D

P

E

G

D

A

A

G

R

I

L

Y

Y

A

Q

T

G

Y
x
A

C
|
C

A

G

R

A

C
|
C

H
|
H

Q

Y

S

N

D

S

G

G

A

P

G

N

V

P

P

V

Q

L

K

G

Y

R

P
|
P

R

E

L
|
L

A

A

G

L

N
|
N

P
x
N

A

A

V

L

L

W

A

L

P

D

Q

E

T

P

T

N

S
x
N

L
|
L

V

Y

R

Q

L
x
V

L
x
M

V

L

E

H

G

G

G

-

S

-

P
x
I

N

T

T

A

D

E

G

E

G
|
G

P
x
Q

E

D

P

H

R
x
I

K
x
S

M
|
M

P
|
P

A

S

F
|
F

A

Y

D

S

K

G

L

L

T

S

D

D

S

H

E

D

L

M

A

A

H

R

V

I

L

A

T

A

F

Y

I

L

R

R

N

R

T

T

binding heme c: Y52 (≠ D39), Y52 (≠ D39), C53 (= C40), C56 (= C43), H57 (= H44), I81 (= I76), S83 (= S78), S84 (= S79), I86 (= I81), W97 (≠ Y92), F102 (= F97), M106 (≠ L101), L117 (= L112), Y118 (= Y113), A120 (= A115), F121 (≠ M116), P122 (= P117), F126 (= F121), L137 (= L132), R163 (= R159), H202 (= H197), C203 (= C198), C206 (= C201), H207 (= H202), W230 (= W230), I231 (≠ F231), A232 (= A232), P233 (= P233), L235 (= L235), N239 (≠ G240), W245 (≠ S246), Y253 (≠ F254), L254 (= L255), G263 (= G260), S264 (≠ G261), A266 (≠ L263), P268 (≠ S268), M269 (= M269), Y292 (≠ L292), A336 (≠ Y334), C337 (= C335), C340 (= C338), H341 (= H339), P353 (= P351), L355 (= L353), N358 (= N356), N359 (≠ P357), N368 (≠ S366), L369 (= L367), V372 (≠ L370), M373 (≠ L371), I377 (≠ P378), G382 (= G383), Q383 (≠ P384), I386 (≠ R387), S387 (≠ K388), M388 (= M389), P389 (= P390), F391 (= F392)
binding ubiquinone-10: E55 (≠ G42), C56 (= C43), F72 (≠ L67), T76 (≠ S71), F78 (= F73), I81 (= I76), Y118 (= Y113), P119 (= P114), A120 (= A115), I160 (≠ F156), I165 (vs. gap), G166 (vs. gap), Q167 (vs. gap), F169 (vs. gap), W170 (= W160), H202 (= H197), A205 (= A200), R210 (= R205), L213 (≠ R212), D228 (= D228), P268 (≠ S268)

8hddB Complex structure of catalytic, small, and a partial electron transfer subunits from burkholderia cepacia fad glucose dehydrogenase
35% identity, 23% coverage: 328:428/433 of query aligns to 23:121/121 of 8hddB

query
sites
8hddB

A

A

G

R

I

L

Y

Y

A

L

T

G

Y
x
N

C
|
C

A

A

R

T

C
|
C

H
|
H

Q

Q

S

M

D

Q

G

G

A

K

G

G

V

T

P

P

Q

D

-

G

K
x
Y

Y
|
Y

P
|
P

R

S

L

L

A

F

G

H

N

N

P

S

A
x
T

V

V

L

G

A

A

P

S

Q

N

T

P

T

S

S

N

L

L

V

V

R

Q

L
x
V

L
x
I

V

L

E

N

G

G

G

-

S

-

P

V

N

Q

T
x
R

D

K

G

I

G

G

P

S

E

E

P

D

R
x
I

K

G

M
|
M

P
|
P

A

A

F
|
F

A

R

D

Y

K

D

L

L

T

N

D

D

S

A

E

Q

L

I

A

A

H

A

V

L

L

T

T

N

F

Y

I

V

R

T

N

A

T

Q

W

F

G

G

N

N

A

P

A

A

E

A

P

K

V

V

T

T

T

E

R

Q

D

D

V

V

S

A

A

K

V

L

R

R

binding protoporphyrin ix containing fe: N29 (≠ Y334), C30 (= C335), C33 (= C338), H34 (= H339), Y45 (≠ K349), Y46 (= Y350), P47 (= P351), T54 (≠ A358), V66 (≠ L370), I67 (≠ L371), R73 (≠ T380), I80 (≠ R387), M82 (= M389), P83 (= P390), F85 (= F392)

6fjaA Crystal structure of t2d three-domain heme-cu nitrite reductase from ralstonia pickettii
32% identity, 24% coverage: 327:428/433 of query aligns to 352:452/455 of 6fjaA

query
sites
6fjaA

G

G

A

R

G

A

I

L

Y

F

A

A

T

G

Y
x
T

C
|
C

A

S

R

V

C
|
C

H
|
H

Q

Q

S

G

D

N

G

G

A

A

G

G

V

L

P

P

Q

G

K

V

Y

F

P
|
P

R
x
P

L
|
L

A

A

G

K

N
x
S

P

D

A
x
F

V
x
L

L

A

A

A

P

D

Q

P

T

K

T

R

S

A

L

M

V

N

R
x
I

L
x
V

L

L

V

H

E

G

G
x
L

G
x
N

G
|
G

S

K

P
x
I

N

K

T
x
V

D

N

G

G

G

Q

P

E

E
x
Y

P
x
D

R
x
S

K
x
V

M
|
M

P

P

A

P

F
x
M

A

T

D

-

K

Q

L

L

T

N

D

D

S

D

E

E

L

V

A

A

H

N

V
x
I

L

L

T

T

F

Y

I

V

R

L

N

N

T

S

W

W

G

D

N

N

A

P

A

G

E

G

P

R

V

V

T

S

T

A

R

E

D

D

V

V

S

K

A

K

V

V

R

R

binding protoporphyrin ix containing fe: T359 (≠ Y334), C360 (= C335), C363 (= C338), H364 (= H339), P376 (= P351), P377 (≠ R352), L378 (= L353), S381 (≠ N356), F383 (≠ A358), L384 (≠ V359), I394 (≠ R369), V395 (≠ L370), L399 (≠ G374), N400 (≠ G375), G401 (= G376), I403 (≠ P378), V405 (≠ T380), Y410 (≠ E385), D411 (≠ P386), S412 (≠ R387), V413 (≠ K388), M414 (= M389), M417 (≠ F392), I428 (≠ V404)

Sites not aligning to the query:

active site: 90, 93, 95, 130, 131, 139, 144, 236, 258, 259, 285
binding copper (ii) ion: 90, 131, 139, 144

4ax3D Structure of three-domain heme-cu nitrite reductase from ralstonia pickettii at 1.6 a resolution (see paper)
32% identity, 24% coverage: 327:428/433 of query aligns to 355:455/457 of 4ax3D

query
sites
4ax3D

G

G

A

R

G

A

I

L

Y

F

A

A

T

G

Y
x
T

C
|
C

A

S

R

V

C
|
C

H
|
H

Q

Q

S

G

D

N

G

G

A

A

G

G

V

L

P

P

Q

G

K

V

Y

F

P
|
P

R
x
P

L
|
L

A

A

G

K

N
x
S

P

D

A
x
F

V
x
L

L

A

A

A

P

D

Q

P

T

K

T

R

S

A

L

M

V

N

R
x
I

L
x
V

L

L

V

H

E

G

G
x
L

G
x
N

G
|
G

S

K

P
x
I

N

K

T
x
V

D

N

G

G

G

Q

P

E

E
x
Y

P
x
D

R
x
S

K
x
V

M
|
M

P

P

A

P

F
x
M

A

T

D

-

K

Q

L

L

T

N

D

D

S

D

E

E

L

V

A

A

H

N

V
x
I

L

L

T

T

F

Y

I

V

R

L

N

N

T

S

W

W

G

D

N

N

A

P

A

G

E

G

P

R

V

V

T

S

T

A

R

E

D

D

V

V

S

K

A

K

V

V

R

R

binding heme c: T362 (≠ Y334), C363 (= C335), C366 (= C338), H367 (= H339), P379 (= P351), P380 (≠ R352), L381 (= L353), S384 (≠ N356), F386 (≠ A358), L387 (≠ V359), I397 (≠ R369), V398 (≠ L370), L402 (≠ G374), N403 (≠ G375), G404 (= G376), I406 (≠ P378), V408 (≠ T380), Y413 (≠ E385), D414 (≠ P386), S415 (≠ R387), V416 (≠ K388), M417 (= M389), M420 (≠ F392), I431 (≠ V404)

Sites not aligning to the query:

active site: 93, 96, 98, 133, 134, 142, 147, 239, 261, 262, 288
binding copper (ii) ion: 93, 98, 133, 134, 142, 147

5ocbA Crystal structure of nitric oxide bound d97n mutant of three-domain heme-cu nitrite reductase from ralstonia pickettii
32% identity, 24% coverage: 327:428/433 of query aligns to 353:453/456 of 5ocbA

query
sites
5ocbA

G

G

A

R

G

A

I

L

Y

F

A

A

T

G

Y
x
T

C
|
C

A

S

R

V

C
|
C

H
|
H

Q

Q

S

G

D

N

G

G

A

A

G

G

V

L

P

P

Q

G

K

V

Y

F

P
|
P

R
x
P

L
|
L

A

A

G

K

N
x
S

P

D

A
x
F

V
x
L

L

A

A

A

P

D

Q

P

T

K

T

R

S

A

L

M

V

N

R
x
I

L
x
V

L

L

V

H

E

G

G
x
L

G
x
N

G
|
G

S

K

P
x
I

N

K

T
x
V

D

N

G

G

G

Q

P

E

E
x
Y

P
x
D

R
x
S

K
x
V

M
|
M

P

P

A

P

F
x
M

A

T

D

-

K

Q

L

L

T

N

D

D

S

D

E

E

L

V

A

A

H

N

V
x
I

L

L

T

T

F

Y

I

V

R

L

N

N

T

S

W

W

G

D

N

N

A

P

A

G

E

G

P

R

V

V

T

S

T

A

R

E

D

D

V

V

S

K

A

K

V

V

R

R

binding heme c: T360 (≠ Y334), C361 (= C335), C364 (= C338), H365 (= H339), P377 (= P351), P378 (≠ R352), L379 (= L353), S382 (≠ N356), F384 (≠ A358), L385 (≠ V359), I395 (≠ R369), V396 (≠ L370), L400 (≠ G374), N401 (≠ G375), G402 (= G376), I404 (≠ P378), V406 (≠ T380), Y411 (≠ E385), D412 (≠ P386), S413 (≠ R387), V414 (≠ K388), M415 (= M389), M418 (≠ F392), I429 (≠ V404)

Sites not aligning to the query:

active site: 91, 94, 96, 131, 132, 140, 145, 237, 259, 260, 286
binding copper (ii) ion: 91, 96, 131, 132, 140, 145
binding nitric oxide: 94, 131

5oboA Crystal structure of nitrite bound d97n mutant of three-domain heme-cu nitrite reductase from ralstonia pickettii
32% identity, 24% coverage: 327:428/433 of query aligns to 353:453/456 of 5oboA

query
sites
5oboA

G

G

A

R

G

A

I

L

Y

F

A

A

T

G

Y
x
T

C
|
C

A

S

R

V

C
|
C

H
|
H

Q

Q

S

G

D

N

G

G

A

A

G

G

V

L

P

P

Q

G

K

V

Y

F

P
|
P

R
x
P

L
|
L

A

A

G

K

N
x
S

P

D

A
x
F

V
x
L

L

A

A

A

P

D

Q

P

T

K

T

R

S

A

L

M

V

N

R
x
I

L
x
V

L

L

V

H

E

G

G
x
L

G
x
N

G
|
G

S

K

P
x
I

N

K

T
x
V

D

N

G

G

G

Q

P

E

E
x
Y

P
x
D

R
x
S

K
x
V

M
|
M

P

P

A

P

F
x
M

A

T

D

-

K

Q

L

L

T

N

D

D

S

D

E

E

L

V

A

A

H

N

V

I

L

L

T

T

F

Y

I

V

R

L

N

N

T

S

W

W

G

D

N

N

A

P

A

G

E

G

P

R

V

V

T

S

T

A

R

E

D

D

V

V

S

K

A

K

V

V

R

R

binding heme c: T360 (≠ Y334), C361 (= C335), C364 (= C338), H365 (= H339), P377 (= P351), P378 (≠ R352), L379 (= L353), S382 (≠ N356), F384 (≠ A358), L385 (≠ V359), I395 (≠ R369), V396 (≠ L370), L400 (≠ G374), N401 (≠ G375), G402 (= G376), I404 (≠ P378), V406 (≠ T380), Y411 (≠ E385), D412 (≠ P386), S413 (≠ R387), V414 (≠ K388), M415 (= M389), M418 (≠ F392)

Sites not aligning to the query:

active site: 91, 94, 96, 131, 132, 140, 145, 237, 259, 260, 286
binding copper (ii) ion: 91, 96, 131, 132, 140, 145
binding nitrite ion: 94, 96, 131

1dt1A Thermus thermophilus cytochrome c552 synthesized by escherichia coli (see paper)
38% identity, 24% coverage: 327:428/433 of query aligns to 2:110/129 of 1dt1A

query
sites
1dt1A

G

G

A

A

G

K

I

I

Y

Y

A

A

T
x
Q

Y

-

C
|
C

A

A

R

G

C
|
C

H
|
H

Q

Q

S

Q

D

N

G

G

A

Q

G

G

V

I

P

P

Q

G

K

A

Y
x
F

P
|
P

R

P

L
|
L

A

A

G

G

N
x
H

P

V

A

A

-

E

V
x
I

L

L

A

A

P

K

Q

E

-

G

T

R

T

E

S
x
Y

L

L

V

I

R

L

L
x
V

L
|
L

V

L

E

Y

G

G

-
x
L

G
x
Q

G
|
G

S

Q

P
x
I

N

E

T

V

D

K

G

G

G

M

P

K

E
x
Y

P
x
N

R
x
G

K
x
V

M
|
M

P

S

A

S

F
|
F

A

A

D

-

K

Q

L

L

T

K

D

D

S

E

E

E

L

I

A

A

H

A

V

V

L

L

T

N

F

H

I
|
I

R

A

N

T

T

A

W

W

G

G

N

D

A

A

A

K

-

K

-

V

-

K

-

G

-

F

E

K

P

P

V

F

T

T

T

A

R

E

D

E

V

V

S

K

A

K

V

L

R

R

binding heme c: Q8 (≠ T333), C9 (= C335), C12 (= C338), H13 (= H339), F24 (≠ Y350), P25 (= P351), L27 (= L353), H30 (≠ N356), I34 (≠ V359), Y43 (≠ S366), V47 (≠ L370), L48 (= L371), L52 (vs. gap), Q53 (≠ G375), G54 (= G376), I56 (≠ P378), Y63 (≠ E385), N64 (≠ P386), G65 (≠ R387), V66 (≠ K388), M67 (= M389), F70 (= F392), I85 (= I408)

Sites not aligning to the query:

binding heme c: 123

1r0qA Characterization of the conversion of the malformed, recombinant cytochrome rc552 to a 2-formyl-4-vinyl (spirographis) heme (see paper)
38% identity, 24% coverage: 327:428/433 of query aligns to 3:111/130 of 1r0qA

query
sites
1r0qA

G

G

A

A

G

K

I

I

Y
|
Y

A

A

T

Q

Y

-

C
|
C

A

A

R

G

C
|
C

H
|
H

Q

Q

S

Q

D

N

G

G

A

Q

G

G

V

I

P

P

Q

G

K

A

Y
x
F

P
|
P

R

P

L
|
L

A

A

G

G

N
x
H

P

V

A

A

-

E

V
x
I

L

L

A

A

P

K

Q

E

-

G

T

R

T

E

S
x
Y

L

L

V

I

R

L

L
x
V

L
|
L

V

L

E

Y

G

G

-
x
L

G
x
Q

G
|
G

S

Q

P
x
I

N

E

T

V

D

K

G

G

G

M

P

K

E
x
Y

P
x
N

R
x
G

K
x
V

M
|
M

P

S

A

S

F
|
F

A

A

D

-

K

Q

L

L

T

K

D

D

S

E

E

E

L

I

A

A

H

A

V
|
V

L

L

T

N

F

H

I
|
I

R

A

N

T

T

A

W

W

G

G

N

D

A

A

A

K

-

K

-

V

-

K

-

G

-

F

E

K

P

P

V

F

T

T

T

A

R

E

D

E

V

V

S

K

A

K

V

L

R

R

binding 2-formyl-protoporphryn ix: Y7 (= Y331), C10 (= C335), C13 (= C338), H14 (= H339), F25 (≠ Y350), P26 (= P351), L28 (= L353), H31 (≠ N356), I35 (≠ V359), Y44 (≠ S366), V48 (≠ L370), L49 (= L371), L53 (vs. gap), Q54 (≠ G375), G55 (= G376), I57 (≠ P378), Y64 (≠ E385), N65 (≠ P386), G66 (≠ R387), V67 (≠ K388), M68 (= M389), F71 (= F392), V82 (= V404), I86 (= I408)

Sites not aligning to the query:

binding 2-formyl-protoporphryn ix: 124

1qyzA Characterization of the malformed, recombinant cytochrome rc552 (see paper)
38% identity, 24% coverage: 327:428/433 of query aligns to 3:111/130 of 1qyzA

query
sites
1qyzA

G

G

A

A

G

K

I

I

Y
|
Y

A

A

T

Q

Y

-

C
|
C

A

A

R

G

C
|
C

H
|
H

Q

Q

S

Q

D

N

G

G

A

Q

G

G

V

I

P

P

Q

G

K

A

Y
x
F

P
|
P

R

P

L
|
L

A

A

G

G

N
x
H

P

V

A

A

-

E

V
x
I

L

L

A

A

P

K

Q

E

-

G

T

R

T

E

S
x
Y

L

L

V

I

R

L

L
x
V

L
|
L

V

L

E

Y

G

G

-
x
L

G
x
Q

G
|
G

S

Q

P
x
I

N

E

T

V

D

K

G

G

G

M

P

K

E
x
Y

P
x
N

R
x
G

K
x
V

M
|
M

P

S

A

S

F
|
F

A

A

D

-

K

Q

L

L

T

K

D

D

S

E

E

E

L

I

A

A

H

A

V

V

L

L

T

N

F

H

I
|
I

R

A

N

T

T

A

W

W

G

G

N

D

A

A

A

K

-

K

-

V

-

K

-

G

-

F

E

K

P

P

V

F

T

T

T

A

R

E

D

E

V

V

S

K

A

K

V

L

R

R

binding 2-acetyl-protoporphyrin ix: Y7 (= Y331), C10 (= C335), C13 (= C338), H14 (= H339), F25 (≠ Y350), P26 (= P351), L28 (= L353), H31 (≠ N356), I35 (≠ V359), Y44 (≠ S366), V48 (≠ L370), L49 (= L371), L53 (vs. gap), Q54 (≠ G375), G55 (= G376), I57 (≠ P378), Y64 (≠ E385), N65 (≠ P386), G66 (≠ R387), V67 (≠ K388), M68 (= M389), F71 (= F392), I86 (= I408)

Sites not aligning to the query:

binding 2-acetyl-protoporphyrin ix: 124

1c52A Thermus thermophilus cytochrome-c552: a new highly thermostable cytochromE-C structure obtained by mad phasing (see paper)
38% identity, 24% coverage: 327:428/433 of query aligns to 4:112/131 of 1c52A

query
sites
1c52A

G

G

A

A

G

K

I

I

Y

Y

A

A

T

Q

Y

-

C
|
C

A

A

R

G

C
|
C

H
|
H

Q

Q

S

Q

D

N

G

G

A

Q

G

G

V

I

P

P

Q

G

K

A

Y
x
F

P
|
P

R

P

L
|
L

A

A

G

G

N
x
H

P

V

A

A

-

E

V
x
I

L

L

A

A

P

K

Q

E

-

G

T

R

T

E

S
x
Y

L

L

V

I

R

L

L
x
V

L
|
L

V

L

E

Y

G

G

-
x
L

G
x
Q

G
|
G

S

Q

P
x
I

N

E

T

V

D

K

G

G

G

M

P

K

E
x
Y

P
x
N

R
x
G

K
x
V

M
|
M

P

S

A

S

F
|
F

A

A

D

-

K

Q

L

L

T

K

D

D

S

E

E

E

L

I

A

A

H

A

V

V

L

L

T

N

F

H

I
|
I

R

A

N

T

T

A

W

W

G

G

N

D

A

A

A

K

-

K

-

V

-

K

-

G

-

F

E

K

P

P

V

F

T

T

T

A

R

E

D

E

V

V

S

K

A

K

V

L

R

R

binding protoporphyrin ix containing fe: C11 (= C335), C14 (= C338), H15 (= H339), F26 (≠ Y350), P27 (= P351), L29 (= L353), H32 (≠ N356), I36 (≠ V359), Y45 (≠ S366), V49 (≠ L370), L50 (= L371), L54 (vs. gap), Q55 (≠ G375), G56 (= G376), I58 (≠ P378), Y65 (≠ E385), N66 (≠ P386), G67 (≠ R387), V68 (≠ K388), M69 (= M389), F72 (= F392), I87 (= I408)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 125

2zooA Crystal structure of nitrite reductase from pseudoalteromonas haloplanktis tac125
30% identity, 27% coverage: 312:428/433 of query aligns to 324:438/438 of 2zooA

query
sites
2zooA

L

F

Q

T

S

K

V

I

E

T

A

A

L

N

K

N

T

K

G

D

E

E

L

Q

E

I

R

R

P

F

G

G

A

Q

G

R

I

V

Y

Y

A

E

T

A

Y
x
N

C
|
C

A

M

R

A

C
|
C

H
|
H

Q

Q

S

A

D

N

G

G

A

E

G

G

V

I

P
|
P

Q
x
G

K
x
A

Y
x
F

P
|
P

R
x
P

L
|
L

A

A

G

K

N
x
S

P

D

A

-

V
x
Y

L
|
L

A

N

P

N

Q

N

T

P

T

L

S

L

L

G

V

V

R

N

L

A

L
x
I

V

I

E

K

G

G

G
x
L

G
x
S

S
x
G

P

P

-
x
I

N

K

T
x
V

D

N

G

N

G

V

P

N

E
x
Y

P
x
N

R
x
G

K
x
V

M
|
M

P

P

A

A

F
x
M

A

-

D

-

K

N

L

L

T

N

D

D

S

E

E

D

L

I

A

A

H

N

V
|
V

L

I

T

T

F

F

I

V

R

L

N

N

T

N

W

W

G

D

N

N

A

A

A

G

E

G

P

K

V

V

T

S

T

A

R

E

D

Q

V

V

S

A

A

K

V

Q

R

R

binding beta-D-fructofuranose: P359 (= P347), G360 (≠ Q348), A361 (≠ K349)
binding protoporphyrin ix containing fe: N346 (≠ Y334), C347 (= C335), C350 (= C338), H351 (= H339), F362 (≠ Y350), P363 (= P351), P364 (≠ R352), L365 (= L353), S368 (≠ N356), Y370 (≠ V359), L371 (= L360), I382 (≠ L371), L386 (≠ G375), S387 (≠ G376), G388 (≠ S377), I390 (vs. gap), V392 (≠ T380), Y397 (≠ E385), N398 (≠ P386), G399 (≠ R387), V400 (≠ K388), M401 (= M389), M404 (≠ F392), V414 (= V404)

Sites not aligning to the query:

active site: 81, 84, 86, 121, 122, 130, 135, 227, 249, 250, 276
binding copper (ii) ion: 81, 86, 121, 122, 130, 135

Query Sequence

>BPHYT_RS10075 FitnessBrowser__BFirm:BPHYT_RS10075
MTLGALASSYTAASAAPGSATSADTAQIARGAYLAKAADCAGCHTAAPRVVRPGTAPIPA
APFAGGLGMASPFGTINSSNITPDPQAGIGLYSYDDFAHALRDGVARGGKRLYPAMPYPS
FAKIDDDDMHALYAYFMHGVRPVATPAPPTRLPFPFNQRWALMFWDWAFVPGERFKPEAG
RDAQWNRGAYLVQSLGHCGACHTPRGPAYEERGYTEASPLYLTGGTNDHWFAPNLTADTG
SGLGRSSAEDIAAFLKHGHGGGLVTFGSMVQVVEDSTQYLDDADLAAIATYLKSLPPRAQ
SGHFNAGSRGALQSVEALKTGELERPGAGIYATYCARCHQSDGAGVPQKYPRLAGNPAVL
APQTTSLVRLLVEGGGSPNTDGGPEPRKMPAFADKLTDSELAHVLTFIRNTWGNAAEPVT
TRDVSAVRHALHK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory