SitesBLAST
Comparing BPHYT_RS10075 FitnessBrowser__BFirm:BPHYT_RS10075 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 14 hits to proteins with known functional sites (download)
Q47945 Alcohol dehydrogenase (quinone), cytochrome c subunit; ADH; Alcohol dehydrogenase (quinone), subunit II; Cytochrome c-553; Cytochrome c553; Ethanol:Q2 reductase; G3-ADH subunit II; Quinohemoprotein-cytochrome c complex; Ubiquinol oxidase; EC 1.1.5.5 from Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans) (see paper)
42% identity, 98% coverage: 3:428/433 of query aligns to 24:434/478 of Q47945
- Q37 (≠ T21) modified: Pyrrolidone carboxylic acid
Sites not aligning to the query:
8gy2B Cryo-em structure of membrane-bound alcohol dehydrogenase from gluconobacter oxydans
43% identity, 94% coverage: 23:428/433 of query aligns to 1:396/433 of 8gy2B
- binding heme c: C18 (= C40), C21 (= C43), H22 (= H44), T46 (≠ S79), I48 (= I81), Y59 (= Y92), L68 (= L101), R73 (≠ A106), V79 (≠ L112), Y80 (= Y113), M83 (= M116), F88 (= F121), R126 (= R159), H165 (= H197), C166 (= C198), C169 (= C201), H170 (= H202), I201 (≠ F231), A202 (= A232), P203 (= P233), L205 (= L235), W216 (≠ S246), F224 (= F254), A234 (≠ V264), V235 (≠ T265), F236 (= F266), F236 (= F266), M239 (= M269), N301 (≠ Y334), C302 (= C335), C305 (= C338), H306 (= H339), M316 (≠ K349), F317 (≠ Y350), P318 (= P351), L320 (= L353), P324 (= P357), G342 (= G375), S352 (≠ E385), V354 (≠ R387), M356 (= M389), F359 (= F392), M375 (≠ I408)
- binding ubiquinone-10: C21 (= C43), L34 (= L67), P39 (= P72), P81 (= P114), L129 (= L162), W132 (= W165), E168 (≠ A200), R173 (= R205), I197 (≠ N227), D241 (≠ Q271)
Sites not aligning to the query:
7w2jC Cryo-em structure of membrane-bound fructose dehydrogenase from gluconobacter japonicus
39% identity, 93% coverage: 28:428/433 of query aligns to 1:386/418 of 7w2jC
- binding heme c: C13 (= C40), C16 (= C43), H17 (= H44), T42 (≠ S79), I44 (= I81), Y55 (= Y92), L75 (= L112), Y76 (= Y113), A78 (= A115), M79 (= M116), R122 (= R159), H161 (= H197), C162 (= C198), C165 (= C201), H166 (= H202), A191 (= A232), P192 (= P233), R223 (≠ V264), P227 (≠ S268), M228 (= M269), V289 (≠ Y334), C290 (= C335), C293 (= C338), H294 (= H339), Y305 (≠ K349), Y306 (= Y350), P307 (= P351), L309 (= L353), N312 (= N356), T313 (≠ P357), T314 (≠ A358), D322 (≠ S366), I327 (≠ L371), V331 (≠ G375), R333 (≠ S377), I340 (≠ R387), M342 (= M389), P343 (= P390), F345 (= F392)
8jejC Cryo-em structure of na-dithionite reduced membrane-bound fructose dehydrogenase from gluconobacter japonicus
39% identity, 93% coverage: 28:428/433 of query aligns to 1:400/413 of 8jejC
- binding heme c: C13 (= C40), C16 (= C43), H17 (= H44), T42 (≠ S79), I44 (= I81), F60 (= F97), L64 (= L101), L75 (= L112), Y76 (= Y113), M79 (= M116), P80 (= P117), Y84 (≠ F121), R122 (= R159), C162 (= C198), C165 (= C201), H166 (= H202), I186 (≠ N227), W189 (= W230), A191 (= A232), P192 (= P233), I194 (≠ L235), W205 (≠ S246), Y213 (≠ F254), R223 (≠ V264), M228 (= M269), V303 (≠ Y334), C304 (= C335), C307 (= C338), H308 (= H339), Y320 (= Y350), P321 (= P351), L323 (= L353), T327 (≠ P357), T328 (≠ A358), D336 (≠ S366), I341 (≠ L371), V345 (≠ G375), R347 (≠ S377), I354 (≠ R387), M356 (= M389), F359 (= F392), I376 (≠ V404)
- binding ubiquinone-10: M36 (≠ F73), P77 (= P114), S124 (≠ A161), W128 (= W165), C165 (= C201), L173 (≠ Y209)
Sites not aligning to the query:
8gy3A Cryo-em structure of membrane-bound aldehyde dehydrogenase from gluconobacter oxydans
34% identity, 94% coverage: 3:411/433 of query aligns to 14:410/440 of 8gy3A
- binding heme c: Y52 (≠ D39), C53 (= C40), C56 (= C43), H57 (= H44), S84 (= S79), I86 (= I81), W97 (≠ Y92), F102 (= F97), L117 (= L112), F121 (≠ M116), F126 (= F121), R163 (= R159), C203 (= C198), C206 (= C201), H207 (= H202), A232 (= A232), P233 (= P233), L235 (= L235), W245 (≠ S246), Y253 (≠ F254), L254 (= L255), G263 (= G260), S264 (≠ G261), M269 (= M269), Y292 (≠ L292), C337 (= C335), C340 (= C338), H341 (= H339), P353 (= P351), L355 (= L353), N358 (= N356), N359 (≠ P357), V372 (≠ L370), I377 (≠ P378), G382 (= G383), Q383 (≠ P384), I386 (≠ R387), M388 (= M389), F391 (= F392)
- binding ubiquinone-10: E55 (≠ G42), T76 (≠ S71), F78 (= F73), Y118 (= Y113), P119 (= P114), I160 (≠ F156), G166 (vs. gap), Q167 (vs. gap), F169 (vs. gap), W170 (= W160), H202 (= H197), R210 (= R205), L213 (≠ R212)
8hddB Complex structure of catalytic, small, and a partial electron transfer subunits from burkholderia cepacia fad glucose dehydrogenase
35% identity, 23% coverage: 328:428/433 of query aligns to 23:121/121 of 8hddB
- binding protoporphyrin ix containing fe: C30 (= C335), C33 (= C338), H34 (= H339), Y46 (= Y350), P47 (= P351), T54 (≠ A358), V66 (≠ L370), I67 (≠ L371), R73 (≠ T380), I80 (≠ R387), M82 (= M389), P83 (= P390)
6fjaA Crystal structure of t2d three-domain heme-cu nitrite reductase from ralstonia pickettii
32% identity, 24% coverage: 327:428/433 of query aligns to 352:452/455 of 6fjaA
- binding protoporphyrin ix containing fe: T359 (≠ Y334), C360 (= C335), C363 (= C338), H364 (= H339), P376 (= P351), P377 (≠ R352), L378 (= L353), F383 (≠ A358), N400 (≠ G375), G401 (= G376), Y410 (≠ E385), S412 (≠ R387), M414 (= M389), M417 (≠ F392)
Sites not aligning to the query:
- active site: 90, 93, 95, 130, 131, 139, 144, 236, 258, 259, 285
- binding copper (ii) ion: 90, 131, 139, 144
4ax3D Structure of three-domain heme-cu nitrite reductase from ralstonia pickettii at 1.6 a resolution (see paper)
32% identity, 24% coverage: 327:428/433 of query aligns to 355:455/457 of 4ax3D
- binding heme c: C363 (= C335), C366 (= C338), H367 (= H339), P379 (= P351), P380 (≠ R352), L381 (= L353), S384 (≠ N356), F386 (≠ A358), N403 (≠ G375), G404 (= G376), S415 (≠ R387), M417 (= M389), M420 (≠ F392)
Sites not aligning to the query:
- active site: 93, 96, 98, 133, 134, 142, 147, 239, 261, 262, 288
- binding copper (ii) ion: 93, 98, 133, 134, 142, 147
5oboA Crystal structure of nitrite bound d97n mutant of three-domain heme-cu nitrite reductase from ralstonia pickettii
32% identity, 24% coverage: 327:428/433 of query aligns to 353:453/456 of 5oboA
- binding heme c: T360 (≠ Y334), C361 (= C335), C364 (= C338), H365 (= H339), P377 (= P351), P378 (≠ R352), L379 (= L353), S382 (≠ N356), F384 (≠ A358), I395 (≠ R369), N401 (≠ G375), G402 (= G376), S413 (≠ R387), M415 (= M389), M418 (≠ F392)
Sites not aligning to the query:
- active site: 91, 94, 96, 131, 132, 140, 145, 237, 259, 260, 286
- binding copper (ii) ion: 91, 96, 131, 132, 140, 145
- binding nitrite ion: 94, 96, 131
1dt1A Thermus thermophilus cytochrome c552 synthesized by escherichia coli (see paper)
38% identity, 24% coverage: 327:428/433 of query aligns to 2:110/129 of 1dt1A
- binding heme c: C9 (= C335), C12 (= C338), H13 (= H339), P25 (= P351), H30 (≠ N356), Y43 (≠ S366), V47 (≠ L370), Q53 (≠ G375), G54 (= G376), G65 (≠ R387), M67 (= M389), F70 (= F392)
Sites not aligning to the query:
1r0qA Characterization of the conversion of the malformed, recombinant cytochrome rc552 to a 2-formyl-4-vinyl (spirographis) heme (see paper)
38% identity, 24% coverage: 327:428/433 of query aligns to 3:111/130 of 1r0qA
- binding 2-formyl-protoporphryn ix: C13 (= C338), H14 (= H339), P26 (= P351), L28 (= L353), H31 (≠ N356), Y44 (≠ S366), V48 (≠ L370), Q54 (≠ G375), G55 (= G376), G66 (≠ R387), M68 (= M389)
Sites not aligning to the query:
1qyzA Characterization of the malformed, recombinant cytochrome rc552 (see paper)
38% identity, 24% coverage: 327:428/433 of query aligns to 3:111/130 of 1qyzA
- binding 2-acetyl-protoporphyrin ix: Y7 (= Y331), C10 (= C335), C13 (= C338), H14 (= H339), P26 (= P351), H31 (≠ N356), Y44 (≠ S366), Q54 (≠ G375), G55 (= G376), G66 (≠ R387), M68 (= M389), F71 (= F392)
Sites not aligning to the query:
1c52A Thermus thermophilus cytochrome-c552: a new highly thermostable cytochromE-C structure obtained by mad phasing (see paper)
38% identity, 24% coverage: 327:428/433 of query aligns to 4:112/131 of 1c52A
- binding protoporphyrin ix containing fe: C11 (= C335), C14 (= C338), H15 (= H339), F26 (≠ Y350), P27 (= P351), L29 (= L353), H32 (≠ N356), Y45 (≠ S366), L54 (vs. gap), Q55 (≠ G375), G56 (= G376), G67 (≠ R387), M69 (= M389), F72 (= F392)
Sites not aligning to the query:
2zooA Crystal structure of nitrite reductase from pseudoalteromonas haloplanktis tac125
30% identity, 27% coverage: 312:428/433 of query aligns to 324:438/438 of 2zooA
- binding protoporphyrin ix containing fe: C347 (= C335), C350 (= C338), H351 (= H339), F362 (≠ Y350), P363 (= P351), P364 (≠ R352), L365 (= L353), S368 (≠ N356), Y370 (≠ V359), I382 (≠ L371), L386 (≠ G375), S387 (≠ G376), G388 (≠ S377), I390 (vs. gap), V392 (≠ T380), Y397 (≠ E385), N398 (≠ P386), G399 (≠ R387), V400 (≠ K388), M401 (= M389)
Sites not aligning to the query:
- active site: 81, 84, 86, 121, 122, 130, 135, 227, 249, 250, 276
- binding copper (ii) ion: 81, 86, 121, 122, 130, 135
Query Sequence
>BPHYT_RS10075 FitnessBrowser__BFirm:BPHYT_RS10075
MTLGALASSYTAASAAPGSATSADTAQIARGAYLAKAADCAGCHTAAPRVVRPGTAPIPA
APFAGGLGMASPFGTINSSNITPDPQAGIGLYSYDDFAHALRDGVARGGKRLYPAMPYPS
FAKIDDDDMHALYAYFMHGVRPVATPAPPTRLPFPFNQRWALMFWDWAFVPGERFKPEAG
RDAQWNRGAYLVQSLGHCGACHTPRGPAYEERGYTEASPLYLTGGTNDHWFAPNLTADTG
SGLGRSSAEDIAAFLKHGHGGGLVTFGSMVQVVEDSTQYLDDADLAAIATYLKSLPPRAQ
SGHFNAGSRGALQSVEALKTGELERPGAGIYATYCARCHQSDGAGVPQKYPRLAGNPAVL
APQTTSLVRLLVEGGGSPNTDGGPEPRKMPAFADKLTDSELAHVLTFIRNTWGNAAEPVT
TRDVSAVRHALHK
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory