SitesBLAST
Comparing BPHYT_RS10675 FitnessBrowser__BFirm:BPHYT_RS10675 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3dgbA Crystal structure of muconate lactonizing enzyme from pseudomonas fluorescens complexed with muconolactone (see paper)
58% identity, 95% coverage: 8:374/385 of query aligns to 5:371/371 of 3dgbA
- active site: H21 (= H24), T52 (= T55), G55 (= G58), T140 (= T143), K166 (= K169), K168 (= K171), D197 (= D200), N199 (= N202), E223 (= E226), D248 (= D251), E249 (= E252), S250 (≠ A253), I251 (≠ L254), K272 (= K275), Y297 (= Y300), G299 (= G302), T300 (= T303), M301 (= M304), H313 (≠ Q316), T325 (= T328), E326 (= E329), L327 (= L330)
- binding magnesium ion: D197 (= D200), E223 (= E226), D248 (= D251)
- binding [(2S)-5-oxo-2,5-dihydrofuran-2-yl]acetic acid: I53 (= I56), Y58 (= Y61), T140 (= T143), K166 (= K169), K168 (= K171), D197 (= D200), K272 (= K275), T300 (= T303), E326 (= E329), F328 (= F331)
1f9cA Crystal structure of mle d178n variant (see paper)
56% identity, 95% coverage: 8:374/385 of query aligns to 4:360/360 of 1f9cA
- active site: T41 (= T55), G44 (= G58), T129 (= T143), K155 (= K169), K157 (= K171), D186 (= D200), N188 (= N202), E212 (= E226), D237 (= D251), E238 (= E252), S239 (≠ A253), K261 (= K275), G288 (= G302), T289 (= T303), M290 (= M304), T314 (= T328), E315 (= E329), L316 (= L330)
- binding manganese (ii) ion: D186 (= D200), E212 (= E226), D237 (= D251)
3fcpA Crystal structure of muconate lactonizing enzyme from klebsiella pneumoniae
47% identity, 95% coverage: 8:374/385 of query aligns to 5:355/356 of 3fcpA
- active site: T41 (= T55), G44 (= G58), T129 (= T143), K151 (= K169), K153 (= K171), D182 (= D200), N184 (= N202), E208 (= E226), D233 (= D251), E234 (= E252), A235 (= A253), K257 (= K275), G284 (= G302), T285 (= T303), M286 (= M304), T309 (= T328), E310 (= E329), M311 (≠ L330)
- binding magnesium ion: N184 (= N202), D233 (= D251), E234 (= E252)
1nu5A Crystal structure of pseudomonas sp. P51 chloromuconate lactonizing enzyme (see paper)
45% identity, 96% coverage: 6:374/385 of query aligns to 1:368/369 of 1nu5A
- active site: L19 (≠ H24), S49 (≠ T55), G52 (= G58), T137 (= T143), K163 (= K169), K165 (= K171), E176 (≠ A182), D194 (= D200), N196 (= N202), E220 (= E226), D245 (= D251), E246 (= E252), S247 (≠ A253), K269 (= K275), G296 (= G302), T297 (= T303), M298 (= M304), C322 (≠ T328), E323 (= E329), L324 (= L330)
- binding manganese (ii) ion: D194 (= D200), E220 (= E226), T235 (= T241), N238 (≠ A244), D245 (= D251)
2chrA A re-evaluation of the crystal structure of chloromuconate cycloisomerase (see paper)
43% identity, 96% coverage: 6:374/385 of query aligns to 1:368/370 of 2chrA
- active site: I19 (≠ H24), S49 (≠ T55), G52 (= G58), T137 (= T143), K163 (= K169), K165 (= K171), D194 (= D200), N196 (= N202), E220 (= E226), D245 (= D251), E246 (= E252), S247 (≠ A253), S267 (≠ A273), K269 (= K275), G296 (= G302), T297 (= T303), M298 (= M304), C322 (≠ T328), E323 (= E329), L324 (= L330)
- binding manganese (ii) ion: D194 (= D200), E220 (= E226), D245 (= D251)
4m0xA Crystal structure of 2-chloromuconate cycloisomerase from rhodococcus opacus 1cp (see paper)
42% identity, 96% coverage: 8:375/385 of query aligns to 3:369/369 of 4m0xA
- active site: T50 (= T55), G53 (= G58), A138 (≠ T143), K163 (= K169), K165 (= K171), D194 (= D200), N196 (= N202), E220 (= E226), D245 (= D251), E246 (= E252), S247 (≠ A253), A267 (= A273), K269 (= K275), A296 (≠ G302), A297 (≠ T303), T298 (≠ M304), C322 (≠ T328), E323 (= E329), L324 (= L330)
- binding manganese (ii) ion: D194 (= D200), E220 (= E226), D245 (= D251)
3i4kA Crystal structure of muconate lactonizing enzyme from corynebacterium glutamicum
38% identity, 96% coverage: 6:374/385 of query aligns to 2:370/370 of 3i4kA
- active site: H20 (= H24), V51 (≠ T55), G54 (= G58), G87 (≠ A91), A139 (≠ T143), K165 (= K169), K167 (= K171), D196 (= D200), N198 (= N202), E222 (= E226), D247 (= D251), E248 (= E252), S249 (≠ A253), A269 (= A273), K271 (= K275), T272 (≠ I276), A298 (≠ G302), T299 (= T303), S300 (≠ M304), T324 (= T328), E325 (= E329), L326 (= L330)
- binding magnesium ion: D196 (= D200), E222 (= E226), D247 (= D251)
1tkkA The structure of a substrate-liganded complex of the l-ala-d/l-glu epimerase from bacillus subtilis (see paper)
32% identity, 93% coverage: 6:362/385 of query aligns to 1:354/359 of 1tkkA
- active site: F19 (≠ H24), P50 (≠ T55), V53 (≠ A60), T135 (= T143), K160 (= K169), K162 (= K171), L190 (≠ V199), D191 (= D200), A192 (≠ V201), N193 (= N202), E219 (= E226), D244 (= D251), E245 (= E252), S246 (≠ A253), N266 (≠ A273), K268 (= K275), G295 (= G302), S296 (≠ T303), M297 (= M304), F320 (= F331), D321 (vs. gap), F322 (vs. gap)
- binding alanine: K160 (= K169), K162 (= K171), D321 (vs. gap), D323 (vs. gap)
- binding glutamic acid: F19 (≠ H24), R24 (≠ A29), K160 (= K169), D191 (= D200), K268 (= K275), S296 (≠ T303), M297 (= M304), I298 (≠ L305)
- binding magnesium ion: D191 (= D200), E219 (= E226), D244 (= D251)
1jpmA L-ala-d/l-glu epimerase (see paper)
32% identity, 93% coverage: 6:362/385 of query aligns to 1:354/359 of 1jpmA
- active site: F19 (≠ H24), P50 (≠ T55), V53 (≠ A60), T135 (= T143), K160 (= K169), K162 (= K171), L190 (≠ V199), D191 (= D200), A192 (≠ V201), N193 (= N202), E219 (= E226), D244 (= D251), E245 (= E252), S246 (≠ A253), N266 (≠ A273), K268 (= K275), G295 (= G302), S296 (≠ T303), M297 (= M304), F320 (= F331), D321 (vs. gap), F322 (vs. gap)
- binding magnesium ion: D191 (= D200), E219 (= E226), D244 (= D251)
O34508 L-Ala-D/L-Glu epimerase; AE epimerase; AEE; EC 5.1.1.20 from Bacillus subtilis (strain 168) (see 2 papers)
32% identity, 93% coverage: 6:362/385 of query aligns to 1:354/366 of O34508
- R24 (≠ A29) binding
- T135 (= T143) binding
- K160 (= K169) binding
- K162 (= K171) active site, Proton acceptor; specific for (R)-substrate epimerization
- D191 (= D200) binding
- E219 (= E226) binding
- D244 (= D251) binding
- K268 (= K275) active site, Proton acceptor; specific for (S)-substrate epimerization
- S296 (≠ T303) binding
- I298 (≠ L305) binding
- D321 (vs. gap) binding
- D323 (vs. gap) binding
3dg6A Crystal structure of muconate lactonizing enzyme from mucobacterium smegmatis complexed with muconolactone (see paper)
30% identity, 96% coverage: 6:375/385 of query aligns to 1:366/366 of 3dg6A
- active site: L19 (≠ H24), P50 (≠ T55), R51 (≠ G57), M134 (≠ T143), K160 (= K169), K162 (= K171), D191 (= D200), N193 (= N202), E217 (= E226), D242 (= D251), E243 (= E252), S244 (≠ A253), K266 (= K275), G292 (= G302), N293 (≠ T303), Q294 (≠ M304), G319 (≠ T328), E320 (= E329), L321 (= L330)
- binding magnesium ion: D191 (= D200), E217 (= E226), D242 (= D251)
- binding [(2S)-5-oxo-2,5-dihydrofuran-2-yl]acetic acid: F21 (≠ L26), T54 (≠ A60), M134 (≠ T143), K160 (= K169), K162 (= K171), D191 (= D200), N193 (= N202), D242 (= D251), K266 (= K275), N293 (≠ T303), Q294 (≠ M304), I295 (≠ L305)
3my9A Crystal structure of a muconate cycloisomerase from azorhizobium caulinodans
31% identity, 93% coverage: 8:364/385 of query aligns to 6:345/357 of 3my9A
- active site: P44 (≠ T55), W45 (≠ I56), S124 (≠ T143), K149 (= K169), K151 (= K171), D180 (= D200), N182 (= N202), E206 (= E226), D231 (= D251), E232 (= E252), S233 (≠ A253), S253 (≠ A273), K255 (= K275), G282 (= G302), T283 (= T303), L284 (≠ M304), C308 (≠ T328), E309 (= E329), F310 (≠ L330)
- binding magnesium ion: E206 (= E226), A221 (≠ T241), L224 (≠ A244), D231 (= D251)
3i6tB Crystal structure of muconate cycloisomerase from jannaschia sp.
32% identity, 82% coverage: 57:372/385 of query aligns to 42:362/364 of 3i6tB
- active site: S136 (≠ T143), K161 (= K169), K163 (= K171), D191 (= D200), N193 (= N202), E217 (= E226), D242 (= D251), E243 (= E252), S262 (≠ A273), K264 (= K275), G291 (= G302), D292 (≠ T303), M293 (= M304), C317 (≠ T328), E318 (= E329), F319 (≠ L330)
- binding magnesium ion: D191 (= D200), E217 (= E226), D242 (= D251)
Sites not aligning to the query:
3i6eA Crystal structure of muconate lactonizing enzyme from ruegeria pomeroyi.
31% identity, 88% coverage: 33:371/385 of query aligns to 18:351/356 of 3i6eA
- active site: S124 (≠ T143), K149 (= K169), K151 (= K171), D179 (= D200), Y180 (≠ V201), N181 (= N202), Q182 (= Q203), E205 (= E226), D230 (= D251), E231 (= E252), S232 (≠ A253), K254 (= K275), Y279 (= Y300), G281 (= G302), D282 (≠ T303), M283 (= M304), C307 (≠ T328), E308 (= E329), F309 (≠ L330)
- binding magnesium ion: D179 (= D200), E205 (= E226), D230 (= D251)
2p8cA Crystal structure of n-succinyl arg/lys racemase from bacillus cereus atcc 14579 complexed with n-succinyl arg. (see paper)
28% identity, 95% coverage: 6:372/385 of query aligns to 1:361/369 of 2p8cA
- active site: F19 (≠ H24), A50 (≠ T55), H53 (≠ G58), V136 (≠ T143), F160 (= F168), K161 (= K169), M162 (≠ L170), K163 (= K171), D191 (= D200), N193 (= N202), E218 (= E226), D243 (= D251), E244 (= E252), G245 (≠ A253), N265 (≠ A273), K267 (= K275), G294 (= G302), S295 (≠ T303), M296 (= M304), V319 (≠ T328), E320 (= E329), L321 (= L330)
- binding magnesium ion: D191 (= D200), E218 (= E226), D243 (= D251)
- binding n~2~-(3-carboxypropanoyl)-l-arginine: F19 (≠ H24), Y26 (≠ M31), M29 (≠ Q34), D51 (≠ I56), K163 (= K171), D191 (= D200), D243 (= D251), K267 (= K275), S295 (≠ T303), M296 (= M304), E320 (= E329), L321 (= L330), T322 (≠ F331)
2p8bA Crystal structure of n-succinyl arg/lys racemase from bacillus cereus atcc 14579 complexed with n-succinyl lys. (see paper)
28% identity, 95% coverage: 6:372/385 of query aligns to 1:361/369 of 2p8bA
- active site: F19 (≠ H24), A50 (≠ T55), H53 (≠ G58), V136 (≠ T143), F160 (= F168), K161 (= K169), M162 (≠ L170), K163 (= K171), D191 (= D200), N193 (= N202), E218 (= E226), D243 (= D251), E244 (= E252), G245 (≠ A253), N265 (≠ A273), K267 (= K275), G294 (= G302), S295 (≠ T303), M296 (= M304), V319 (≠ T328), E320 (= E329), L321 (= L330)
- binding magnesium ion: D191 (= D200), E218 (= E226), D243 (= D251)
- binding n-succinyl lysine: F19 (≠ H24), Y26 (≠ M31), D51 (≠ I56), V136 (≠ T143), K161 (= K169), K163 (= K171), D243 (= D251), K267 (= K275), S295 (≠ T303), M296 (= M304), E320 (= E329), L321 (= L330), T322 (≠ F331)
2p88A Crystal structure of n-succinyl arg/lys racemase from bacillus cereus atcc 14579 (see paper)
28% identity, 95% coverage: 6:372/385 of query aligns to 1:361/369 of 2p88A
- active site: F19 (≠ H24), A50 (≠ T55), H53 (≠ G58), V136 (≠ T143), F160 (= F168), K161 (= K169), M162 (≠ L170), K163 (= K171), D191 (= D200), N193 (= N202), E218 (= E226), D243 (= D251), E244 (= E252), G245 (≠ A253), N265 (≠ A273), K267 (= K275), G294 (= G302), S295 (≠ T303), M296 (= M304), V319 (≠ T328), E320 (= E329), L321 (= L330)
- binding magnesium ion: D191 (= D200), E218 (= E226), D243 (= D251)
Q81IL5 N-succinyl-L-Arg/Lys racemase; N-succinyl amino acid racemase; NSAR; EC 5.1.1.- from Bacillus cereus (strain ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NCTC 2599 / NRRL B-3711) (see paper)
28% identity, 95% coverage: 6:372/385 of query aligns to 1:361/369 of Q81IL5
- D191 (= D200) binding
- E218 (= E226) binding
- D243 (= D251) binding
A0A0P0ZBS7 o-succinylbenzoate synthase; OSB synthase; OSBS; 4-(2'-carboxyphenyl)-4-oxybutyric acid synthase; N-acylamino acid racemase; NAAAR; N-succinylamino acid racemase; NSAAR; NSAR; o-succinylbenzoic acid synthase; EC 4.2.1.113; EC 5.1.1.- from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
27% identity, 91% coverage: 23:371/385 of query aligns to 20:361/375 of A0A0P0ZBS7
- K166 (= K171) mutation to A: Almost loss of activity with N-formyl-D/L-methionine.
- D191 (= D200) mutation to A: Almost loss of activity with N-formyl-D/L-methionine.
- E216 (= E226) mutation to A: Almost loss of activity with N-formyl-D/L-methionine.
- D241 (= D251) mutation to A: Almost loss of activity with N-formyl-D/L-methionine.
- K265 (= K275) mutation to A: Almost loss of activity with N-formyl-D/L-methionine.
4h2hA Crystal structure of an enolase (mandalate racemase subgroup, target efi-502101) from pelagibaca bermudensis htcc2601, with bound mg and l-4-hydroxyproline betaine (betonicine) (see paper)
28% identity, 94% coverage: 6:365/385 of query aligns to 2:357/368 of 4h2hA
- active site: Y21 (≠ H24), S137 (≠ T143), Q162 (≠ K169), K164 (= K171), D194 (= D200), E219 (= E226), D242 (= D251), E243 (= E252), K266 (= K275), D293 (vs. gap), D294 (= D298), A295 (≠ L299), E318 (≠ W326), G319 (= G327), A320 (≠ T328), W321 (≠ E329)
- binding (2S,4R)-4-hydroxy-1,1-dimethylpyrrolidinium-2-carboxylate: Y21 (≠ H24), Y57 (= Y61), Q162 (≠ K169), K164 (= K171), D242 (= D251), K266 (= K275), A295 (≠ L299), W321 (≠ E329)
- binding magnesium ion: D194 (= D200), E219 (= E226), D242 (= D251)
Query Sequence
>BPHYT_RS10675 FitnessBrowser__BFirm:BPHYT_RS10675
MIATPVKIESVDTILVDVPTIRPHRLSVATMNCQTLVLVRIRCADGLIGVGEGTTIGGLA
YGEESPESIKVNIDTYFAPLLKGMDATRPGAAMAKLRELFQGNRFAKSALETALFDAQAQ
RLGVPLSELFGGRVRDSVEVAWTLASGDTQRDIDEAEQVYEAKRHRVFKLKIGTRAVADD
VAHVVAIQKAVQGRGEVRVDVNQAWSESDAIWAARRFADAGVTLIEQPIAAENRAGLKRL
TDLALVPIMADEALHGPADAFVVASARAADVFAVKIAQSGGLVGAAHVAAIASAANIDLY
GGTMLEGAVGTIASAQLFSTFGELKWGTELFGPLLLTEEILTEPLRYENFALHLPEGPGL
GVTLDWDKIDRLRRDTRKGASVTTN
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory