SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS11300 BPHYT_RS11300 2-dehydro-3-deoxygluconokinase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q97U29 2-dehydro-3-deoxygluconokinase/2-dehydro-3-deoxygalactonokinase; 2-dehydro-3-deoxyglucono/galactono-kinase; 2-keto-3-deoxy-galactonokinase; 2-keto-3-deoxygluconokinase; 3-deoxy-2-oxo-D-gluconate kinase; KDG kinase; KDGal kinase; EC 2.7.1.178 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
33% identity, 92% coverage: 8:308/327 of query aligns to 2:300/313 of Q97U29

query
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Q97U29

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GSELN 34:38 (≠ GADLN 40:44) binding
Y90 (≠ F95) binding
YYR 106:108 (≠ YFR 111:113) binding
NIR 164:166 (≠ NLR 173:175) binding
R166 (= R175) binding
KLGSKG 226:231 (≠ KLGAQG 233:238) binding
GAGD 255:258 (= GAGD 263:266) binding
D258 (= D266) binding
D294 (= D302) binding

2varA Crystal structure of sulfolobus solfataricus 2-keto-3-deoxygluconate kinase complexed with 2-keto-3-deoxygluconate (see paper)
33% identity, 92% coverage: 8:308/327 of query aligns to 1:299/311 of 2varA

query
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2varA

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active site: G254 (= G263), A255 (= A264), G256 (= G265), D257 (= D266)
binding adenosine monophosphate: K225 (= K233), G227 (= G235), S228 (≠ A236), G230 (= G238), A231 (= A239), A244 (≠ Q253), Y245 (≠ P254), V249 (= V258), A255 (= A264), G256 (= G265), M259 (≠ F268), I281 (≠ N290), S284 (≠ G293), I288 (= I297)
binding phosphoaminophosphonic acid-adenylate ester: N163 (= N173), R165 (= R175), K225 (= K233), G227 (= G235), S228 (≠ A236), G230 (= G238), A231 (= A239), A244 (≠ Q253), Y245 (≠ P254), V249 (= V258), T253 (≠ V262), G254 (= G263), A255 (= A264), G256 (= G265), D257 (= D266), M259 (≠ F268), I281 (≠ N290), S284 (≠ G293), I288 (= I297)
binding 3-deoxy-alpha-D-erythro-hex-2-ulofuranosonic acid: L10 (≠ M17), A32 (= A39), G33 (= G40), S34 (≠ A41), N37 (= N44), Y89 (≠ F95), L103 (≠ I109), Y105 (= Y111), R107 (= R113), I136 (≠ V142), R165 (= R175), T253 (≠ V262), G254 (= G263), D257 (= D266), D293 (= D302)
binding 2-keto-3-deoxygluconate: L10 (≠ M17), G33 (= G40), S34 (≠ A41), N37 (= N44), Y89 (≠ F95), L103 (≠ I109), Y105 (= Y111), R107 (= R113), I136 (≠ V142), R165 (= R175), T253 (≠ V262), G254 (= G263), D257 (= D266), D293 (= D302)

1v1bA 2-keto-3-deoxygluconate kinase from thermus thermophilus with bound atp (see paper)
34% identity, 94% coverage: 8:315/327 of query aligns to 2:300/300 of 1v1bA

query
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1v1bA

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active site: G248 (= G263), A249 (= A264), G250 (= G265), D251 (= D266)
binding adenosine-5'-triphosphate: N165 (= N173), S193 (vs. gap), K219 (= K233), G221 (= G235), G224 (= G238), A238 (≠ P254), F239 (≠ V255), V241 (vs. gap), P246 (≠ T261), V247 (= V262), G248 (= G263), A249 (= A264), G250 (= G265), D251 (= D266), F253 (= F268), N275 (= N290), G278 (= G293), A279 (= A294), A282 (≠ I297)

Q53W83 2-dehydro-3-deoxygluconokinase; 2-keto-3-deoxygluconokinase; 3-deoxy-2-oxo-D-gluconate kinase; KDG kinase; EC 2.7.1.45 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
34% identity, 94% coverage: 8:315/327 of query aligns to 2:300/309 of Q53W83

query
sites
Q53W83

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R

I

V

S

S

F

L

D

D

P

V

N

N

L

Y

R
|
R

P

Q

T

T

L

L

W

W

P

S

S

P

R

E

A

E

A

A

M

R

V

-

E

-

G

-

L

-

N

-

A

-

L

-

A

-

A

G

L

F

A

L

D

E

W

R

V

A

L

L

P

P

G

G

I

V

G

D

-

L

-

F

-

L

-
x
S

-

E

E

E

G

A

E

E

I

L

L

L

T

F

G

G

Y

R

T

V

Q

E

P

E

D

A

D

L

I

R

A

A

K

-

F

-

Y

-

L

-

E

-

Q

L

G

S

A

A

R

P

G

E

V

V

I

V

I

L

K
|
K

L
x
R

G
|
G

A
|
A

Q
x
K

G
|
G

A
|
A

Y

W

F

-

R

-

T

A

A

F

D

V

D

D

A

G

A

R

M

R

I

V

A

E

G

G

Q

S

P

A

V

F

A

A

-

V

K

E

V

A

V

V

D

D

T

P

V

V

G
|
G

A
|
A

G
|
G

D
|
D

G

A

F

F

A

A

V

A

G

G

V

Y

V

L

S

A

A

G

L

A

L

V

E

W

G

G

K

L

S

P

L

V

P

E

Q

E

A

R

V

L

A

R

R

L

G

A

N
|
N

R

L

I

L

G

G

A

A

L

S

A

V

I

A

Q

A

V

S

I

R

G

G

D
|
D

S

H

E

E

G

G

L

A

P

P

S

Y

R

R

A

E

E

D

L

L

D

E

A

V

L

L

GAEVN 34:38 (≠ GADLN 40:44) binding
YYR 103:105 (≠ YFR 111:113) binding
R167 (= R175) binding
S193 (vs. gap) binding
219:225 (vs. 233:239, 71% identical) binding
GAGD 248:251 (= GAGD 263:266) binding
D251 (= D266) binding
N275 (= N290) binding
D287 (= D302) binding

1v1aA 2-keto-3-deoxygluconate kinase from thermus thermophilus with bound 2- keto-3-deoxygluconate and adp (see paper)
34% identity, 94% coverage: 8:315/327 of query aligns to 2:300/301 of 1v1aA

query
sites
1v1aA

L

L

D

E

V

V

I

V

T

T

Y

A

G

G

E

E

A

P

M
x
L

A

V

M

A

F

L

V

V

A

P

A

Q

E

E

T

P

G

G

P

H

L

L

A

R

G

G

V

K

G

R

Q

L

F

L

T

E

K

V

R

Y

V

V

A
x
G

G
|
G

A
|
A

D

E

L

V

N
|
N

V

V

A

A

I

V

G

A

L

L

S

A

R

R

L

L

G

G

F

V

K

K

V

V

G

G

W

F

M

V

S

G

R

R

V

V

G

G

N

E

D

D

S

E

F

L

G

G

Q

A

Y

M

V

V

R

E

D

E

T

R

L

L

T

R

K

A

E

E

G

G

I

V

D

D

Q

-

G

-

C

-

V

L

T

T

T

H

D

F

E

R

R

R

Y

A

P

P

-

G

-

F

T

T

G

G

F

L

Q
x
Y

L

L

K

R

S

E

K

Y

N

L

D

P

D

L

G

G

S

Q

D

G

P

-

A

R

I

V

E

F

Y
|
Y

F

Y

R
|
R

K

K

G

G

S

S

A

A

A

G

S

S

H

A

L

L

S

A

L

P

A

G

D

A

Y

F

A

D

A

P

D

D

Y

Y

V

L

L

E

Q

G

A

V

R

R

H

F

L

L

H

H

L

L

T

S

G

G

V
x
I

A

T

P

P

A

A

I

L

S

S

A

P

S

E

S

A

R

R

E

A

L

F

A

S

F

L

H

W

L

A

A

M

R

E

E

E

M

A

R

K

A

R

G

G

K

V

T

R

I

V

S

S

F

L

D

D

P

V

N

N

L

Y

R
|
R

P

Q

T

T

L

L

W

W

P

S

S

P

R

E

A

E

A

A

M

R

V

-

E

-

G

-

L

-

N

-

A

-

L

-

A

-

A

G

L

F

A

L

D

E

W

R

V

A

L

L

P

P

G

G

I

V

G

D

-

L

-

F

-

L

-

S

-

E

E

E

G

A

E

E

I

L

L

L

T

F

G

G

Y

R

T

V

Q

E

P

E

D

A

D

L

I

R

A

A

K

-

F

-

Y

-

L

-

E

-

Q

L

G

S

A

A

R

P

G

E

V

V

I

V

I

L

K
|
K

L

R

G
|
G

A
|
A

Q

K

G

G

A

A

Y

W

F

-

R

-

T

A

A

F

D

V

D

D

A

G

A

R

M

R

I

V

A

E

G

G

Q

S

P

A

V
x
F

A

A

-
x
V

K

E

V
x
A

V

V

D

D

T

P

V

V

G
|
G

A
|
A

G
|
G

D
|
D

G

A

F
|
F

A

A

V

A

G

G

V

Y

V

L

S

A

A

G

L

A

L

V

E

W

G

G

K

L

S

P

L

V

P

E

Q

E

A

R

V

L

A

R

R

L

G

A

N
|
N

R

L

I

L

G
|
G

A
|
A

L

S

A

V

I
x
A

Q

A

V

S

I

R

G

G

D
|
D

S

H

E

E

G

G

L

A

P

P

S

Y

R

R

A

E

E

D

L

L

D

E

A

V

L

L

active site: G248 (= G263), A249 (= A264), G250 (= G265), D251 (= D266)
binding adenosine-5'-diphosphate: K219 (= K233), G221 (= G235), A222 (= A236), F239 (≠ V255), V241 (vs. gap), A243 (≠ V258), A249 (= A264), G250 (= G265), F253 (= F268), N275 (= N290), G278 (= G293), A279 (= A294), A282 (≠ I297)
binding 2-keto-3-deoxygluconate: L11 (≠ M17), G33 (≠ A39), G34 (= G40), A35 (= A41), N38 (= N44), Y89 (≠ Q96), Y103 (= Y111), R105 (= R113), I134 (≠ V142), R167 (= R175), G248 (= G263), D251 (= D266), D287 (= D302)

2dcnA Crystal structure of 2-keto-3-deoxygluconate kinase from sulfolobus tokodaii complexed with 2-keto-6-phosphogluconate (alpha-furanose form)
30% identity, 94% coverage: 10:315/327 of query aligns to 3:304/308 of 2dcnA

query
sites
2dcnA

V

L

I

I

T

T

Y

L

G

G

E

E

A

I

M
x
L

A

I

M

E

F

F

V

N

A

A

A

L

E

S

T

P

G

G

P

P

L

L

A

R

G

H

V

V

G

S

Q

Y

F

F

T

E

K

K

R

H

V

V

A
|
A

G
|
G

A
x
S

D

E

L

A

N
|
N

V

Y

A

C

I

V

G

A

L

F

S

I

R

K

L

Q

G

G

F

N

K

E

V

C

G

G

W

I

M

I

S

A

R

K

V

V

G

G

N

D

D

D

S

E

F

F

G

G

Q

Y

Y

N

V

A

R

I

D

E

T

W

L

L

T

R

K

G

E

Q

G

G

I

V

D

D

Q

V

G

S

C

H

V

M

T

K

T

I

D

D

E

P

R

S

Y

A

P

P

T

T

G

G

-

I

F
|
F

Q

F

L

I

K

Q

S

R

K

H

N

Y

D

P

D

V

G

P

S

L

D

K

P

S

A

E

I

S

E

I

Y
|
Y

F

Y

R
|
R

K

K

G

G

S

S

A

A

A

G

S

S

H

K

L

L

S

S

L

P

A

E

D

D

Y

V

A

D

A

E

D

E

Y

Y

V

V

L

K

Q

S

A

A

R

D

H

L

L

V

H

H

L

S

T

S

G

G

V
x
I

A

T

P

L

A

A

I

I

S

S

A

S

S

T

S

A

R

K

E

E

L

-

A

A

F

V

H

Y

L

K

A

A

R

F

E

E

M

I

R

A

A

S

A

-

G

-

K

-

T

N

I

R

S

S

F

F

D

D

P

T

N

N

L

I

R
|
R

P

L

T

K

L

L

W

W

P

S

S

A

R

E

A

E

A

A

M

K

V

R

E

E

G

I

L

L

N

K

A

L

L

L

A

S

A

K

L

F

A

H

-

L

D

K

W

F

V

L

L

I

P

T

G
x
D

I

T

G

D

E

D

G

S

E

K

I

I

L

I

T

L

G

G

Y

E

T

S

Q

D

P

P

D

D

D

K

I

A

A

A

K

K

F

A

Y

F

L

S

E

D

Q

Y

G

-

A

A

R

E

G

I

V

I

I

V

I

M

K
|
K

L
|
L

G
|
G

A
x
P

Q

K

G
|
G

A

A

Y

I

F

V

R

Y

T

Y

A

D

D

G

D

K

A

K

A

Y

M

Y

I

S

A

S

G

G

Q
x
Y

P

Q

V

V

A

P

K

-

V
|
V

V

E

D

D

T
x
V

V
x
T

G
|
G

A
|
A

G
|
G

D
|
D

G

A

F
x
L

A

G

V

G

G

T

V

F

V

L

S

S

A

L

L

Y

E

K

G

G

K

F

S

E

L

M

P

E

Q

K

A

A

V

L

A

D

R

Y

G

A

N
x
I

R

V

I

A

G
x
S

A

T

L

L

A

N

I
x
V

Q

M

V

I

I

R

G

G

D
|
D

S

Q

E

E

G

N

L

L

P

P

S

T

R

T

A

K

E

D

L

I

D

E

A

T

L

F

active site: G252 (= G263), A253 (= A264), G254 (= G265), D255 (= D266)
binding adenosine-5'-diphosphate: D193 (≠ G202), K223 (= K233), L224 (= L234), G225 (= G235), P226 (≠ A236), G228 (= G238), Y243 (≠ Q253), V247 (= V258), V250 (≠ T261), A253 (= A264), G254 (= G265), L257 (≠ F268), I279 (≠ N290), S282 (≠ G293), V286 (≠ I297)
binding 6-O-phosphono-beta-D-psicofuranosonic acid: L10 (≠ M17), A32 (= A39), G33 (= G40), S34 (≠ A41), N37 (= N44), F89 (= F95), Y105 (= Y111), R107 (= R113), I136 (≠ V142), R165 (= R175), T251 (≠ V262), G252 (= G263), A253 (= A264), G254 (= G265), D255 (= D266), D291 (= D302)

3in1A Crystal structure of a putative ribokinase in complex with adp from e.Coli
28% identity, 94% coverage: 7:315/327 of query aligns to 1:307/312 of 3in1A

query
sites
3in1A

S

N

L

L

D

D

V

V

I

I

T

C

Y

I

G

G

E

A

A

A

M

I

A

V

-

D

-

I

-

P

-

L

-

Q

-

P

-

V

-

S

-

K

M

N

F

I

V

F

A

D

A

V

E

D

T

S

G

Y

P

P

L

L

A

E

G

R

V

I

G

A

Q

M

F

T

T

T

K

-

R

-

V

-

A

G

G

G

A

D

D

A

L

I

N

N

V

E

A

A

I

T

G

I

L

I

S

S

R

R

L

L

G

G

F

H

K

R

V

T

G

A

W

L

M

M

S

S

R

R

V

I

G

G

N

K

D

D

S

A

F

A

G

G

Q

Q

Y

F

V

I

R

L

D

D

T

H

L

C

T

R

K

K

E

E

G

N

I

I

D

D

Q

I

G

Q

C

S

V

L

T

K

T

Q

D

D

E

V

R

S

Y

I

P

D

T

T

G

S

F

I

Q

N

L

V

K

G

S

L

K

V

N

T

D

E

D

D

G

G

S

E

D
x
R

P

T

A

F

I

V

E

T

Y

N

F

-

R

R

K

N

G

G

S

S

A

L

A

W

S

-

H

K

L

L

S

N

L

I

A

D

D

D

Y

V

A

D

A

F

D

A

Y

R

V

F

L

S

Q

Q

A

A

R

K

H

L

L

L

H

S

L

L

T

A

G

S

V

I

-

F

-

N

A

S

P

P

A

L

I

L

S

D

A

G

S

K

S

A

R

L

E

T

L

E

A

I

F

F

H

T

L

Q

A

A

R

K

E

-

M

-

R

-

A

-

A

A

G

R

K

Q

T

M

I

I

S

I

F

C

D

A

P

D

N

M

L

I

R

K

P

P

T

R

L

L

W

N

P

E

S

T

R

L

A

D

M

I

V

C

E

E

G

A

L

L

N

-

A

-

L

-

A

-

A

S

L

Y

A

V

D

D

W

Y

V

L

L

F

P

P

G
x
N

I

F

G

A

E

E

G

A

E

K

I

L

L

L

T

T

G

G

Y

K

T

E

Q

T

P

L

D

D

D

E

I

I

A

A

K

D

F

C

Y

F

L

L

E

A

Q

C

G

G

A

V

R

K

G

T

V

V

I

V

I

I

K
|
K

L
x
T

G
|
G

A
x
K

Q

D

G
|
G

A

C

Y

F

F

I

R

K

T

R

A

G

D

D

D

M

A

T

A

M

M

K

I

V

A

P

G
x
A

Q

V

P

A

V

G

A
x
I

K

T

V

A

V

I

D

D

T
|
T

V

I

G
|
G

A
|
A

G
|
G

D
|
D

G

N

F
|
F

A

A

V

S

G

G

V

F

V

I

S

A

A

A

L

L

E

E

G

G

K

K

S

N

L

L

P

R

Q

E

A

C

V

A

A

R

R

F

G

A

N
|
N

R

A

I

T

G
x
A

A

A

L

I

A

S

I

V

Q

L

V

S

I

V

G

G

D

A

S

T

E

T

G

G

L

V

P

K

S

N

R

R

A

K

E

L

L

V

D

E

A

Q

L

L

active site: R106 (≠ D106), G255 (= G263), A256 (= A264), G257 (= G265), D258 (= D266)
binding adenosine-5'-diphosphate: N194 (≠ G202), K225 (= K233), T226 (≠ L234), G227 (= G235), K228 (≠ A236), G230 (= G238), A244 (≠ G252), I248 (≠ A256), T253 (= T261), A256 (= A264), G257 (= G265), F260 (= F268), N282 (= N290), A285 (≠ G293)

5eynA Crystal structure of fructokinase from vibrio cholerae o395 in fructose, adp, beryllium trifluoride and calcium ion bound form
30% identity, 87% coverage: 30:315/327 of query aligns to 18:304/306 of 5eynA

query
sites
5eynA

G

G

V

Q

G

Q

Q

H

F

Y

T

L

K

K

R

C

V

P

A
x
G

G
|
G

A
|
A

D

P

L

A

N
|
N

V

V

A

A

I

V

G

A

L

I

S

A

R

R

L

L

G

S

F

G

K

R

V

S

G

A

W

F

M

F

S

G

R

R

V

V

G

G

N

N

D

D

S

P

F

F

G

G

Q

R

Y

F

V

M

R

Q

D

Q

T

T

L

L

T

T

K

D

E

E

G

Q

I

V

D

D

Q

C

G

Q

C

H

V

L

T

H

T

F

D

D

E

P

R

V

Y

H

P

R

T

T

G

S

F

T

Q
x
V

L

V

K

V

S

D

K

L

N

D

D

E

D
x
H

G

G

S

-

D

E

P

R

A

S

I
x
F

E

T

Y
x
F

F

M

R

V

K

K

G

P

S

S

A

A

A

D

S

Q

H

F

L

L

S

Q

L

L

A

S

D

D

Y

I

A

P

A

S

D

-

Y

-

V

F

L

Q

Q

K

A

G

R

E

H

W

L

L

H

H

L

V

T

C

G

S

V
x
I

A

A

P

L

A

A

I

-

S

N

A

Q

S

P

S

S

R

R

E

S

L

S

A

T

F

F

H

A

L

A

A

I

R

A

E

Q

M

M

R

K

A

E

A

V

G

G

K

G

T

Y

I

V

S

S

F

F

D

D

P

P

N
|
N

L

L

R
|
R

P

E

T

E

L

V

W

W

P

S

S

E

R

P

A

Q

A

E

M

L

V

Q

E

A

G

T

L

V

N

M

A

R

L

A

A

V

A

G

L

L

A

A

D

D

W

V

V

V

L

K

P

F

G

S

I

E

G

E

E

E

G

L

E

Q

I

F

L

L

T

T

G

G

Y

T

T

Q

Q

S

P

I

D

E

-

G

-

L

-

Q

-

A

I

I

A

A

K

D

F

F

Y

Q

L

I

E

P

Q

L

G

-

A

-

R

-

G

-

V

V

I

V

K
x
T

L

L

G
|
G

A
|
A

Q

K

G
|
G

A

A

Y

L

F

V

R

A

T

T

A

P

D

N

D

S

A

Q

M

I

I

V

A

S

G

G

Q

K

P
x
A

V
|
V

A

-

K

K

V
x
P

V

I

D

D

T
|
T

V
x
T

G
|
G

A
|
A

G
|
G

D
|
D

G

A

F
|
F

A

V

V

G

G

G

V

L

S

Y

A

R

L

L

L

S

E

V

G

A

K

Q

-

D

-

W

-

H

-

N

-

Q

-

A

S

T

L

I

P

L

Q

D

A

A

V

V

A

K

R

W

G

A

N
|
N

R

G

I

C

G
|
G

A
|
A

L

L

A

A

I

T

Q

T

V

Q

I

K

G

G

D

A

S

M

E

T

G

A

L

L

P

P

S

N

R

Q

A

A

E

A

L

L

D

Y

A

A

L

F

active site: G246 (= G263), A247 (= A264), G248 (= G265), D249 (= D266)
binding adenosine-5'-diphosphate: H91 (≠ D103), T217 (≠ K233), G219 (= G235), A220 (= A236), G222 (= G238), A238 (≠ P254), V239 (= V255), P241 (≠ V258), T244 (= T261), G246 (= G263), A247 (= A264), G248 (= G265), F251 (= F268), N279 (= N290), G282 (= G293), A283 (= A294)
binding beryllium trifluoride ion: N157 (= N173), R159 (= R175), T245 (≠ V262), G246 (= G263), A247 (= A264), G248 (= G265), D249 (= D266)
binding beta-D-fructofuranose: G27 (≠ A39), G28 (= G40), A29 (= A41), N32 (= N44), V84 (≠ Q96), F96 (≠ I109), F98 (≠ Y111), I127 (≠ V142), N157 (= N173), R159 (= R175), G246 (= G263), D249 (= D266)

Sites not aligning to the query:

binding beta-D-fructofuranose: 9, 11, 13

5yggA Crystal structure of fructokinase double-mutant (t261c-h108c) from vibrio cholerae o395 in fructose, adp and potassium ion bound form (see paper)
30% identity, 87% coverage: 30:315/327 of query aligns to 22:308/310 of 5yggA

query
sites
5yggA

G

G

V

Q

G

Q

Q

H

F

Y

T

L

K

K

R

C

V

P

A
x
G

G
|
G

A
|
A

D

P

L

A

N
|
N

V

V

A

A

I

V

G

A

L

I

S

A

R

R

L

L

G

S

F

G

K

R

V

S

G

A

W

F

M

F

S

G

R

R

V

V

G

G

N

N

D

D

S

P

F

F

G

G

Q

R

Y

F

V

M

R

Q

D

Q

T

T

L

L

T

T

K

D

E

E

G

Q

I

V

D

D

Q

C

G

Q

C

H

V

L

T

H

T

F

D

D

E

P

R

V

Y

H

P

R

T

T

G

S

F

-

Q

T

L
x
V

K

V

S

V

K

D

N

L

D

D

D

E

G

C

S

G

D

E

P

R

A

S

I
x
F

E

T

Y
x
F

F

M

R

V

K

K

G

P

S

S

A

A

A

D

S

Q

H

F

L

L

S

Q

L

L

A

S

D

D

Y

I

A

P

A

S

D

-

Y

-

V

F

L

Q

Q

K

A

G

R

E

H

W

L

L

H

H

L

V

T

C

G

S

V
x
I

A

A

P

L

A

A

I

-

S

N

A

Q

S

P

S

S

R

R

E

S

L

S

A

T

F

F

H

A

L

A

A

I

R

A

E

Q

M

M

R

K

A

E

A

V

G

G

K

G

T

Y

I

V

S

S

F

F

D

D

P

P

N
|
N

L

L

R
|
R

P

E

T

E

L

V

W

W

P

S

S

E

R

P

A

Q

A

E

M

L

V

Q

E

A

G

T

L

V

N

M

A

R

L

A

A

V

A

G

L

L

A

A

D

D

W

V

V

V

L
x
K

P

F

G
x
S

I

E

G

E

E

E

G

L

E

Q

I

F

L

L

T

T

G

G

Y

T

T

Q

Q

S

P

I

D

E

-

G

-

L

-

Q

-

A

I

I

A

A

K

D

F

F

Y

Q

L

I

E

P

Q

L

G

-

A

-

R

-

G

-

V

V

I

V

K
x
T

L

L

G
|
G

A
|
A

Q

K

G
|
G

A

A

Y

L

F

V

R

A

T

T

A

P

D

N

D

S

A

Q

M

I

I

V

A

S

G

G

Q
x
K

P
x
A

V
|
V

A

-

K

K

V

P

V

I

D

D

T

C

V

T

G

G

A
|
A

G
|
G

D
|
D

G

A

F
|
F

A

V

V

G

G

G

V

L

S

Y

A

R

L

L

L

S

E

V

G

A

K

Q

-

D

-

W

-

H

-

N

-

Q

-

A

S

T

L

I

P

L

Q

D

A

A

V

V

A

K

R

W

G

A

N
|
N

R

G

I

C

G
|
G

A
|
A

L

L

A

A

I

T

Q

T

V

Q

I

K

G

G

D

A

S

M

E

T

G

A

L

L

P

P

S

N

R

Q

A

A

E

A

L

L

D

Y

A

A

L

F

binding adenosine-5'-diphosphate: K188 (≠ L200), S190 (≠ G202), T221 (≠ K233), G223 (= G235), A224 (= A236), G226 (= G238), K241 (≠ Q253), A242 (≠ P254), V243 (= V255), A251 (= A264), G252 (= G265), F255 (= F268), N283 (= N290), G286 (= G293), A287 (= A294)
binding beta-D-fructofuranose: G31 (≠ A39), G32 (= G40), A33 (= A41), N36 (= N44), V88 (≠ L97), F100 (≠ I109), F102 (≠ Y111), I131 (≠ V142), N161 (= N173), R163 (= R175), D253 (= D266)

Sites not aligning to the query:

binding beta-D-fructofuranose: 13, 15, 17

3ih0A Crystal structure of an uncharacterized sugar kinase ph1459 from pyrococcus horikoshii in complex with amp-pnp
32% identity, 82% coverage: 12:279/327 of query aligns to 5:255/304 of 3ih0A

query
sites
3ih0A

T

S

Y

I

G

G

E

E

A

L

M

L

A

I

M

D

F

L

V

I

A

S

A

V

E

E

T

E

G

G

P

D

L

L

A

K

G

D

V

V

G

R

Q

L

F

F

T

E

K

K

R

H

V

P

A

G

G

G

A

A

D

P

L

A

N

N

V

V

A

A

I

V

G

G

L

V

S

S

R

R

L

L

G

G

F

V

K

K

V

S

G

S

W

L

M

I

S

S

R

K

V

V

G

G

N

N

D

D

S

P

F

F

G

G

Q

E

Y

Y

V

L

R

I

D

E

T

E

L

L

T

S

K

K

E

E

G

N

I

V

D

D

Q

T

G

R

C

G

V

I

T

V

T

K

D

D

E

E

R

K

Y

K

P

H

T

T

G

G

F

I

-

V

-

F

-

V

Q

Q

L

L

K

K

S

-

K

-

N

-

D

-

G

G

S

A

D

S

P

P

A

S

I

F

E

L

Y

L

F

Y

R

-

K

D

G

D

S

V

A

A

A

Y

S

F

H

N

L

M

S

T

L

L

A

N

D

D

Y

I

A

N

A

W

D

D

Y

I

V

V

L

E

Q

E

A

A

R

K

H

I

L

V

H

N

L

F

T

G

G

S

V

V

A

I

P

L

A

A

I

-

S

R

A

N

S

P

S

S

R

R

E

E

L

T

A

V

F

M

H

K

L

V

A

I

R

K

E

K

M

I

R

K

A

G

A

S

G

S

K

-

T

L

I

I

S

A

F

F

D

D

P

V

N

N

L

L

R

R

P

L

T

D

L

L

W

W

P

R

-

G

S

Q

R

E

A

E

M

M

V

I

E

K

G

V

L

L

N

E

A

E

L

S

A

I

A

K

L

L

A

A

D

D

W

I

V

V

L

-

P

-

G
x
K

I

A

G

S

E

E

G

E

E

E

I

V

L

L

T

Y

G

L

Y

E

T

N

Q

Q

P

G

D

V

D

E

I

V

A

-

K

-

F

-

Y

-

L

-

E

-

Q

K

G

G

A

S

R

M

G

L

V

T

I

A

I

I

K
x
T

L

L

G
|
G

A
x
P

Q

K

G
|
G

A

-

Y

-

F
|
F

R

R

T

L

A

I

D

K

D

N

A

E

A

T

M

V

I

V

A

-

G

D

Q

V

P

P

V
x
S

A

Y

K

N

V
|
V

-

N

-
x
P

V

L

D

D

T
|
T

V

T

G
|
G

A
|
A

G
|
G

D
|
D

G

A

F

F

A

-

V

-

G

-

V

-

V

M

S

A

A

A

L

L

E

V

G

G

active site: G243 (= G263), A244 (= A264), G245 (= G265), D246 (= D266)
binding phosphoaminophosphonic acid-adenylate ester: K189 (≠ G202), T214 (≠ K233), G216 (= G235), P217 (≠ A236), G219 (= G238), F220 (= F241), S233 (≠ V255), V236 (= V258), P238 (vs. gap), T241 (= T261), A244 (= A264), G245 (= G265)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 275, 279

3gbuA Crystal structure of an uncharacterized sugar kinase ph1459 from pyrococcus horikoshii in complex with atp
32% identity, 82% coverage: 12:279/327 of query aligns to 4:254/302 of 3gbuA

query
sites
3gbuA

T

S

Y

I

G

G

E

E

A

L

M

L

A

I

M

D

F

L

V

I

A

S

A

V

E

E

T

E

G

G

P

D

L

L

A

K

G

D

V

V

G

R

Q

L

F

F

T

E

K

K

R

H

V

P

A

G

G

G

A

A

D

P

L

A

N

N

V

V

A

A

I

V

G

G

L

V

S

S

R

R

L

L

G

G

F

V

K

K

V

S

G

S

W

L

M

I

S

S

R

K

V

V

G

G

N

N

D

D

S

P

F

F

G

G

Q

E

Y

Y

V

L

R

I

D

E

T

E

L

L

T

S

K

K

E

E

G

N

I

V

D

D

Q

T

G

R

C

G

V

I

T

V

T

K

D

D

E

E

R

K

Y

K

P

H

T

T

G

G

F

I

-

V

-

F

-

V

Q

Q

L

L

K

K

S

-

K

-

N

-

D

-

G

G

S

A

D

S

P

P

A

S

I

F

E

L

Y

L

F

Y

R

-

K

D

G

D

S

V

A

A

A

Y

S

F

H

N

L

M

S

T

L

L

A

N

D

D

Y

I

A

N

A

W

D

D

Y

I

V

V

L

E

Q

E

A

A

R

K

H

I

L

V

H

N

L

F

T

G

G

S

V

V

A

I

P

L

A

A

I

-

S

R

A

N

S

P

S

S

R

R

E

E

L

T

A

V

F

M

H

K

L

V

A

I

R

K

E

K

M

I

R

K

A

G

A

S

G

S

K

-

T

L

I

I

S

A

F

F

D

D

P

V

N

N

L

L

R

R

P

L

T

D

L

L

W

W

P

R

-

G

S

Q

R

E

A

E

M

M

V

I

E

K

G

V

L

L

N

E

A

E

L

S

A

I

A

K

L

L

A

A

D

D

W

I

V

V

L

-

P

-

G
x
K

I

A

G

S

E

E

G

E

E

E

I

V

L

L

T

Y

G

L

Y

E

T

N

Q

Q

P

G

D

V

D

E

I

V

A

-

K

-

F

-

Y

-

L

-

E

-

Q

K

G

G

A

S

R

M

G

L

V

T

I

A

I

I

K
x
T

L

L

G
|
G

A
x
P

Q

K

G
|
G

A

-

Y

-

F
|
F

R

R

T

L

A

I

D

K

D

N

A

E

A

T

M

V

I

V

A

-

G

D

Q

V

P

P

V
x
S

A

Y

K

N

V
|
V

-

N

-
x
P

V

L

D

D

T
|
T

V

T

G
|
G

A
|
A

G
|
G

D
|
D

G

A

F

F

A

-

V

-

G

-

V

-

V

M

S

A

A

A

L

L

E

V

G

G

active site: G242 (= G263), A243 (= A264), G244 (= G265), D245 (= D266)
binding adenosine-5'-triphosphate: K188 (≠ G202), T213 (≠ K233), G215 (= G235), P216 (≠ A236), G218 (= G238), F219 (= F241), S232 (≠ V255), V235 (= V258), P237 (vs. gap), T240 (= T261), A243 (= A264), G244 (= G265)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 274, 278

3iq0B Crystal structure of a putative ribokinase ii in complex with atp and mg+2 from e.Coli
27% identity, 94% coverage: 10:315/327 of query aligns to 4:304/308 of 3iq0B

query
sites
3iq0B

V

V

I

F

T

T

Y

I

G

G

E

E

A

I

M

L

A

V

M

E

F

I

V

M

A

A

A

S

E

K

T

I

G

G

-

Q

P

P

L

F

A

D

G

Q

V

P

G

G

Q

I

F

W

T

N

-

G

K

P

R

Y

V

P

A

S

G

G

A

A

D

P

L

A

N

I

V

F

A

I

I

D

G

Q

L

V

S

T

R

R

L

L

G

G

F

V

K

P

V

C

G

G

W

I

M

I

S

S

R

C

V

V

G

G

N

N

D

D

S

G

F

F

G

G

Q

D

Y

I

V

N

R

I

D

H

T

R

L

L

T

A

K

A

E

D

G

G

I

V

D

D

Q

I

G

R

C

G

V

I

T

S

T

V

D

L

E

P

R

L

Y

E

P

A

T

T

G

G

F

S

Q

A

L

F

K

V

S

T

K

Y

N

G

D

D

D

R

G

D

S

-

D

-

P

-

A

-

I

F

E

I

Y

F

F

N

R

I

K

K

G

N

S

A

A

A

A

C

S

G

H

K

L

L

S

S

L

A

A

Q

D

H

Y

V

A

D

A

E

D

N

Y

I

V

L

L

K

Q

D

A

C

R

T

H

H

L

F

H

H

L

I

T

M

G

G

V

-

A

-

P

-

A

-

I

-

S

-

A

-

S

-

S

S

R

S

E

L

L

F

A

S

F

F

H

H

L

M

A

V

R

D

E

A

M

V

R

K

A

K

A

A

-

V

-

T

-

I

-

V

-

K

-

A

-

N

G

G

K

G

T

V

I

I

S

S

F

F

D

D

P

P

N
|
N

L

I

R

R

P

K

T

E

L

M

W

L

P

-

S

D

R

I

A

P

A

E

M

M

V

R

E

D

G

A

L

L

N

H

A

F

L

V

A

L

A

E

L

L

A

T

D

D

W

I

V

Y

L

M

P

P

G
x
S

I

E

G

G

E

E

G

V

E

L

I

L

L

L

T

S

G

P

Y

H

T

S

Q

T

P

P

D

E

D

R

I

A

A

I

K

A

F

G

Y

F

L

L

E

E

Q

E

G

G

A

V

R

K

G

E

V

V

I

I

I

V

K
|
K

L
x
R

G
|
G

A
x
N

Q

Q

G
|
G

A

A

Y

S

F

Y

R

Y

T

S

A

A

D

N

D

E

A

Q

A

F

M

H

I

V

A

E

G

S

Q
x
Y

P

P

V
|
V

A

E

K
x
E

V

-

V

V

D

D

T

P

V

T

G
|
G

A
|
A

G
|
G

D
|
D

G

C

F
|
F

A

G

V

G

G

A

V

W

V

I

S

A

A

C

L

R

L

Q

E

L

G

G

K

F

S

D

L

A

P

H

Q

R

A

A

V

L

A

Q

R

Y

G

A

N
|
N

R

A

I

C

G
|
G

A
|
A

L

L

A

A

I
x
V

Q

T

V

R

I

R

G

G

D

P

S

M

E

E

G

G

L

T

P

S

S

R

R

L

A

M

E

E

L

I

D

E

A

T

L

F

active site: G252 (= G263), A253 (= A264), G254 (= G265), D255 (= D266)
binding adenosine-5'-triphosphate: N164 (= N173), S192 (≠ G202), K223 (= K233), R224 (≠ L234), G225 (= G235), N226 (≠ A236), G228 (= G238), Y243 (≠ Q253), V245 (= V255), E247 (≠ K257), A253 (= A264), G254 (= G265), F257 (= F268), N279 (= N290), G282 (= G293), A283 (= A294), V286 (≠ I297)

4ebuA Crystal structure of a sugar kinase (target efi-502312) from oceanicola granulosus, with bound amp/adp crystal form i
30% identity, 84% coverage: 8:282/327 of query aligns to 15:282/306 of 4ebuA

query
sites
4ebuA

L

M

D

H

V

I

I

L

T

S

Y

I

G

G

E

E

A

C

M

M

A

A

M

-

F

-

V

-

A

-

E

E

T

L

G

A

P

P

L

A

A

D

G

L

V

P

G

G

Q

T

F

Y

T

R

K

L

R

G

V

F

A

A

G

G

A

D

D

T

L

F

N

N

V

T

A

A

I

W

G

Y

L

L

S

A

R

R

L

L

-

R

-

P

G

E

F

S

K

R

V

I

G

S

W

Y

M

F

S

S

R

A

V

I

G

G

N

D

D

D

S

A

F

L

G

S

Q

Q

Y

Q

V

M

R

R

D

A

T

A

L

M

T

S

K

A

E

A

G

G

I

I

D

D

Q

G

G

G

C

G

V

L

T

R

T

V

D

I

E

P

R

G

Y

R

P

T

T

V

G

G

F

L

Q

Y

L

L

K

I

S

T

K

L

N

R

D

-

G

-

S

-

D

-

P

-

A

S

I

F

E

A

Y

Y

F

W

R

R

K

G

G

Q

S

S

A

A

S

R

H

E

L

L

S

A

L

G

A

D

D

A

Y

D

A

A

A

L

D

A

Y

A

V

A

L

M

Q

A

A

R

R

A

H

D

L

V

-

V

H

Y

L

F

T

S

G

G

V

I

A

T

P

L

A

A

I

I

-

L

S

D

A

Q

S

C

S

G

R

R

E

A

L

T

A

L

F

L

H

R

L

A

A

L

R

A

E

Q

M

A

R

R

A

A

A

T

G

G

K

R

T

T

I

I

S

A

F

F

D

D

P

P

N

N

L

L

R

R

P

P

T

R

L

L

W

W

P

A

S

G

R

T

A

G

A

E

M

M

V

T

E

E

G

T

L

I

N

M

A

Q

L

G

A

A

L

V

A

S

D

D

W

I

V

A

L

L

P

P

G
x
S

I

F

G

E

E

D

G

E

E

A

I

A

L

W

T

F

G

G

Y

D

T

A

Q

G

P

P

D

D

D

A

I

T

A

A

K

D

F

R

Y

Y

L

A

E

R

Q

A

G

G

A

V

R

R

G

S

V

V

I

V

K
|
K

L

N

G
|
G

A
x
P

Q

H

G

A

A

V

Y

H

F

F

R

L

T

Q

A

D

D

G

D

R

A

R

A

G

M

R

I

V

A

P

G
x
V

Q

P

P

P

V
|
V

A

A

K

Q

V

V

D

D

T
|
T

V

T

G
x
A

A
|
A

G
|
G

D
|
D

G

S

F
|
F

A

N

V

A

G

G

V

L

S

D

A

S

L

V

L

L

E

A

G

G

K

Q

S

P

L

L

active site: A263 (≠ G263), A264 (= A264), G265 (= G265), D266 (= D266)
binding adenosine-5'-diphosphate: S202 (≠ G202), K233 (= K233), G235 (= G235), P236 (≠ A236), V252 (≠ G252), V255 (= V255), T261 (= T261), A264 (= A264), G265 (= G265), F268 (= F268)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 290, 293

Q8ZKR2 Aminoimidazole riboside kinase; AIRs kinase; EC 2.7.1.223 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
28% identity, 88% coverage: 34:320/327 of query aligns to 25:311/319 of Q8ZKR2

query
sites
Q8ZKR2

F

Y

T

L

K

K

R

C

V

P

A

G

G
|
G

A

A

D

S

L

A

N

N

V

V

A

G

I

V

G

C

L

V

S

A

R

R

L

L

G

G

F

G

K

E

V

C

G

G

W

F

M

I

S

G

R

C

V

L

G

G

N

D

D

D

S

D

F

A

G

G

Q

R

Y

F

V

L

R

R

D

Q

T

V

L

F

T

Q

K

D

E

N

G

G

I

V

D

D

Q

V

G

T

C

F

V

L

T

R

T

L

D

D

E

A

R

D

Y

L

P

T

T

S

G

A

F

V

Q

L

L

I

K

V

S

N

K

L

N

T

D

A

D

D

G

G

S

-

D

E

P

R

A

S

I

F

E

T

Y
|
Y

F

L

R

V

K

H

G

P

S

G

A

A

A

D

S

T

H

Y

L

V

S

S

L

P

A

Q

D

D

Y

L

A

P

D

F

Y

R

V

Q

L

Y

Q

E

A

W

R

F

H

Y

L

F

H

S

L

S

T

I

G

G

V

L

A

T

P

-

A

-

I

-

S

D

A

R

S

P

S

A

R

R

E

E

L

A

A

C

F

L

H

E

L

G

A

A

R

R

E

R

M

M

R

R

A

E

A

A

G

G

K

G

T

Y

I

V

S

L

F

F

D

D

P

V

N

N

L

L

R
|
R

P

S

T

K

L

M

W

W

P

G

S

N

R

T

A

D

A

E

M

I

V

P

E

E

G

L

L

I

N

A

A

R

L

S

A
|
A

L

L

A
|
A

D

S

W

I

V

C

L

K

P

V

G

S

I

A

G

D

E

E