SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS11800 BPHYT_RS11800 acetate permease to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

P07117 Sodium/proline symporter; Proline carrier; Proline permease; Propionate transporter from Escherichia coli (strain K12) (see 4 papers)
24% identity, 91% coverage: 23:501/528 of query aligns to 18:470/502 of P07117

query
sites
P07117

L

M

V

I

L

L

T

I

G

G

W

F

S

I

A

A

R

W

R

R

S

S

-

T

R

K

G

N

T

F

S

D

G

D

F

Y

F

I

V

L

A

G

G

G

R

R

N

S

L

L

S

G

P

P

F

F

Q

V

N

T

G

A

L

L

A

S

L

A

A

G

G

A

D

S

F

D

M

M

S

S

A

G

G

W

S

L

F

L

L

M

G

G

V

L

T

P

G

G

L

A

V

V

S

F

L

L

F

S

G

G

F

I

D

S

G

E

I

S

V

W

Y

I

Q

A

V

I

G

G

F

L

I

T

A

L

G

G

-

A

W

W

I

I

M

N

I

W

M

K

L

L

I

V

V

A

A

G

-

R

-

L

-

R

-

V

E

H

P

T

V

E

R

Y

N

N

C

N

G

N

K

A

Y

L

T

T

L

L

S

P

D

D

V

Y

V

F

A

T

L

G

R

R

L

F

-

E

-

D

R

K

S

S

R

R

G

I

V

L

R

R

A

I

A

I

T

S

S

A

V

L

S

V

S

I

L

L

I

L

T

F

S

F

L

T

A

I

Y

Y

L

C

L

A

A

S

Q

G

L

I

V

V

G

A

G

G

G

A

A

R

L

L

A

F

S

E

L

S

L

T

L

F

P

G

I

M

G

S

T

Y

N

E

A

T

A

A

I

L

V

W

L

A

I

G

G

A

I

A

L

A

M

T

I

I

A

L

Y

Y

V

T

L

F

F

I

G

G

G

G

M

F

V

L

A

A

A

V

T

S

Y

W

V

T

Q

D

I

T

V

V

K

Q

A

A

V

S

L

L

V

M

W

I

T

F

A

A

-

L

-

I

-

L

G

T

A

P

V

V

L

I

V

V

L

I

L

I

A

S

L

V

A

G

H

G

F

F

S

G

F

-

D

D

V

S

G

L

A

E

L

V

F

I

N

K

K

Q

A

K

R

S

L

I

S

E

S

N

L

V

H

-

P

-

A

-

Q

-

Y

-

F

-

V

-

P

-

G

-

G

-

Y

-

F

-

K

-

D

D

P

M

L

L

D

K

T

G

L

L

S

N

L

F

T

V

-

A

-

I

-

I

-

S

-

L

L

M

A

G

L

W

A

G

L

L

G

G

T

Y

A

F

G

G

L

Q

P

P

H

H

V

I

M

L

T

A

R
|
R

F

F

Y

M

T

A

V

A

P

D

S

S

A

H

V

H

A

S

A

I

R

V

K

H

S

A

A

R

K

R

I

I

G

S

L

M

I

T

V

W

M

M

T

I

S

L

F
x
C

A

L

V

A

M

G

M

A

I

V

L

A

L

V

G

G

F

F

S

F

A

G

T

I

A

A

I

Y

V

F

G

N

-

D

-

H

P

P

A

A

A

L

I

A

L

G

A

A

T

V

H

N

S

Q

S

N

G

A

N

E

S

R

A

V

I

F

T

I

L

E

L

L

A

A

T

Q

T

I

L

L

G

F

G

N

G

P

A

W

G

-

S

-

A

I

G

A

G

G

E

I

L

L

L

L

L

S

A

A

C

I

V

L

S

A

A

A

I

V

A

-

F

-

A

-

T

-

I

-

L

M

A

S

V

T

V

L

S

S

G
x
C

I

Q

M

L

I

L

S

V

S
x
C

A

S

S

S

T

A

I

I

S

T

H

E

D

D

I

L

F

Y

G

-

Q

K

L

A

F

F

S

L

A

R

S

K

R

H

D

A

K

S

E

Q

R

K

R

E

Q

L

V

V

V

W

I

V

A

G

K
x
R

I

V

A

M

T

V

I

L

V

V

F

V

G

A

I

L

A

V

A

A

M

I

G

A

L

L

A

A

L

A

L

N

V

P

K

E

T

N

F

-

N

R

V

V

A

L

F

G

L

L

V

V

G

S

L

Y

A

A

F

W

A

A

I

G

A

F

A

G

S

A

A

A

N

F

L

G

P

P

V

V

I

V

L

L

F

F

S

S

M

V

Y

M

W

W

R

S

R

R

F

M

T

T

D

R

R

N

G

G

A

A

V

L

A

A

A

G

V

M

L

I

A

I

G

G

T

A

L

L

S

T

S

V

I

I

G

V

L

W

V

K

L

Q

L

F

G

G

P

-

A

-

V

-

I

-

G

-

A

-

K

-

G

-

I

-

I

-

F

-

K

-

D

-

S

-

T

-

P

-

P

-

F

-

W

W

L

L

S

-

N

-

P

-

G

G

L

L

F

Y

S

E

I

I

P

I

V

P

G

G

F

F

I

I

A

F

G

G

W

S

I

I

G

G

S

I

V

V

T

V

T

F

S

S

R257 (= R260) mutation to C: Sodium-independent binding affinity for proline.
C281 (≠ F284) mutation to S: Does not affect proline uptake activity. Confers resistance to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.
C344 (≠ G352) mutation to S: Small decrease in proline uptake activity. Confers resistance to N-ethylmaleimide. Exhibits low Na(+)-dependent proline binding.
C349 (≠ S357) mutation to S: Does not affect proline uptake activity. Sensitive to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.
R376 (≠ K385) mutation R->E,Q: No change in activity.; mutation to K: Loss of activity.

Q9GZV3 High affinity choline transporter 1; Hemicholinium-3-sensitive choline transporter; CHT; Solute carrier family 5 member 7 from Homo sapiens (Human) (see 3 papers)
22% identity, 74% coverage: 12:404/528 of query aligns to 8:401/580 of Q9GZV3

query
sites
Q9GZV3

I

L

I

I

A

A

F

I

V

I

G

V

F

F

I

Y

G

L

L

L

S

I

L

L

V

L

L

V

T

G

G

I

W

W

S

A

A

A

R

W

R

R

S

T

R

K

G

N

T

S

-

G

-

S

-

A

-

E

-

E

-

R

-

S

S

E

G

A

F

I

F

I

V

V

A

G

G

G

R

R

N
x
D

L

I

S

G

P

L

F

L

Q

V

N

G

G

G

L

F

A

T

L

M

A

T

G

A

D

T

F

W

M

V

S

G

A
x
G

G

G

S

Y

F

I

L

N

G

G

V

T

T

A

G

E

L

A

V

V

S

Y

L

V

F

P

G

G

F

Y

D

-

G

G

I

L

V

A

Y

W

-

A

-

Q

-

A

Q

P

V
x
I

G

G

F

Y

I

S

A

L

G

S

W

L

I

I

M

L

I

G

M

G

L

L

I

F

V

F

A

A

E

K

P
|
P

V

M

R

R

N

S

C

K

G

G

K
x
Y

Y

V

T

T

L

M

S

L

D

D

V

P

V

F

A

Q

L

-

R

Q

L

I

R

Y

S

G

R

K

G

R

V

M

R

G

A

G

A

L

T

L

S

F

V

I

S

P

S

A

L

L

I

M

T

G

S

E

L

M

A

F

Y

W

L

A

L

A

A

A

Q

I

L

F

V

S

G

A

G

L

G

G

A

A

L

T

A

I

S

S

L

V

L

I

L

I

P

D

I

V

G

D

T

M

N

H

A

I

A

S

I

V

V

I

L

I

I

S

G

A

I

L

L

I

M

A

I

T

A

L

Y
|
Y

V

T

L

L

F

V

G

G

G

G

M

L

V

Y

A

S

A

V

T

A

Y

Y

V

T

Q

D

I

V

V

V

K

Q

A

L

V

F

L

C

V

I

W

F

T

V

A

G

G

L

A

W

V

I

L

S

V

V

L

P

L

F

A

A

L

L

A

S

H

H

F

P

S

A

F

V

-

A

D

D

V

I

G

G

-

F

-

T

-

A

-

V

-

H

A

A

L

K

F

Y

N

Q

K

K

A

P

R

W

L

L

S

G

S

T

L

V

H

D

P

S

A

S

Q

E

Y

V

F

Y

V

-

P

-

G

-

G

-

Y

-

F

-

K

-

D

S

P

W

L

L

D

D

T

S

L

F

S

-

L

-

T

-

L

L

A

L

L

L

A

M

L

L

G

G

T

G

A

I

G

P

L

W

P

Q

H

A

V

Y

M

F

T

Q

R

R

F

V

Y

L

T

S

V

S

P

S

S

S

A

A

V

T

A

Y

A

A

R

Q

K

V

-

L

-

S

-

F

S

L

A

A

K

A

I

F

G

G

L

C

I

L

V

V

M

M

T

A

S

I

F

P

A

A

V

I

M

L

M
x
I

I

G

L

A

L

I

G

G

F

-

S

A

A

S

T

T

A

D

I

W

V

N

G

Q

P

T

A

A

A

Y

I

G

L

L

A

P

T

D

H

P

S

K

S

T

G

T

N

E

S

E

A

A

I

D

T

M

L

I

L

L

A

P

T

I

T

V

L

L

G

Q

G

-

G

-

A

-

G

-

S

-

A

-

G

-

G

-

E

-

L

-

L

-

L

Y

A

L

C

C

V

P

S

V

A

Y

I

I

A

S

F

F

A

F

T

G

I

L

L

G

A

A

V

V

V

S

S

A

G

A

I
x
V

M

M

I

S

S

S

S

A

A

D

S

S

T

S

I

I

-

L

-

S

-

A

-

S

-

S

-

M

-

F

S

A

H
x
R

D

N

I

I

F

Y

G

-

Q

Q

L

L

F

S

S

F

A

R

S

Q

R

N

D

A

K

S

E

D

R

K

R

E

Q

I

V

V

V

W

I

V

A

M

K

R

I

I

A

T

T

V

I

F

V

V

F

F

G

G

I

A

A

S

A

A

M

T

G

A

L

M

A

A

L

L

L

L

V

T

K

K

T

T

D48 (≠ N45) to G: in CMS20; decreased choline transmembrane transporter activity; no effect on localization at plasma membrane; dbSNP:rs886039768
G65 (≠ A62) to E: in CMS20; loss of choline transmembrane transporter activity; no effect on localization at plasma membrane; dbSNP:rs886039765
I89 (≠ V84) to V: 40% reduction in choline uptake rate; found in 0.06 of Ashkenazi Jews; dbSNP:rs1013940; mutation to A: Only 20% of wild-type choline uptake activity.
P105 (= P100) to S: in CMS20; decreased choline transmembrane transporter activity; no effect on localization at plasma membrane; dbSNP:rs886039766
Y111 (≠ K106) to H: in CMS20; no effect on localization at plasma membrane
Y175 (= Y171) to C: in CMS20; unknown pathological significance; dbSNP:rs1331713195
I291 (≠ M288) to T: in CMS20; unknown pathological significance; dbSNP:rs375397889
V344 (≠ I353) to L: in CMS20; unknown pathological significance
R361 (≠ H363) to Q: in CMS20; decreased choline transmembrane transporter activity; no effect on localization at plasma membrane; dbSNP:rs147656110

Sites not aligning to the query:

418 F → V: in CMS20; unknown pathological significance
446 R → G: in CMS20; decreased choline transmembrane transporter activity; no effect on localization at plasma membrane
451 E→Q: Only 5% of wild-type choline uptake activity.

Query Sequence

>BPHYT_RS11800 BPHYT_RS11800 acetate permease
MNNLISHGGLRIIAFVGFIGLSLVLTGWSARRSRGTSGFFVAGRNLSPFQNGLALAGDFM
SAGSFLGVTGLVSLFGFDGIVYQVGFIAGWIMIMLIVAEPVRNCGKYTLSDVVALRLRSR
GVRAATSVSSLITSLAYLLAQLVGGGALASLLLPIGTNAAIVLIGILMIAYVLFGGMVAA
TYVQIVKAVLVWTAGAVLVLLALAHFSFDVGALFNKARLSSLHPAQYFVPGGYFKDPLDT
LSLTLALALGTAGLPHVMTRFYTVPSAVAARKSAKIGLIVMTSFAVMMILLGFSATAIVG
PAAILATHSSGNSAITLLATTLGGGAGSAGGELLLACVSAIAFATILAVVSGIMISSAST
ISHDIFGQLFSASRDKERRQVVIAKIATIVFGIAAMGLALLVKTFNVAFLVGLAFAIAAS
ANLPVILFSMYWRRFTDRGAVAAVLAGTLSSIGLVLLGPAVIGAKGIIFKDSTPPFWLSN
PGLFSIPVGFIAGWIGSVTTSQNAADTSFDAQQLRMLSGLGAEAASEH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory