SitesBLAST

P0AC81 Lactoylglutathione lyase; Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase; EC 4.4.1.5 from Escherichia coli (strain K12) (see paper)
37% identity, 94% coverage: 3:126/132 of query aligns to 2:125/135 of P0AC81

query
sites
P0AC81

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H5 (= H6) binding
E56 (= E53) binding
H74 (= H72) binding in other chain
E122 (= E123) binding in other chain

2c21A Specificity of the trypanothione-dependednt leishmania major glyoxalase i: structure and biochemical comparison with the human enzyme (see paper)
37% identity, 93% coverage: 3:125/132 of query aligns to 3:120/139 of 2c21A

query
sites
2c21A

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active site: H6 (= H6), E57 (= E53), H75 (= H72), E118 (= E123)
binding nickel (ii) ion: H6 (= H6), E57 (= E53), H75 (= H72), E118 (= E123)

6bnnA Crystal structure of v278e-glyoxalase i mutant from zea mays in space group p4(1)2(1)2 (see paper)
35% identity, 93% coverage: 4:126/132 of query aligns to 147:273/282 of 6bnnA

query
sites
6bnnA

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binding cobalt (ii) ion: Q149 (≠ H6), E200 (= E53)
binding glutathione: R153 (= R10), Y183 (≠ F38), N204 (= N57)

Sites not aligning to the query:

Q948T6 Lactoylglutathione lyase; Aldoketomutase; Allergen Glb33; Glyoxalase I; Glx I; Glyoxylase I 11; OsGLYI-11; OsGLYI11; Ketone-aldehyde mutase; Methylglyoxalase; PP33; S-D-lactoylglutathione methylglyoxal lyase; Allergen Ory s Glyoxalase I; EC 4.4.1.5 from Oryza sativa subsp. japonica (Rice) (see paper)
35% identity, 93% coverage: 4:126/132 of query aligns to 156:282/291 of Q948T6

query
sites
Q948T6

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E209 (= E53) mutation to D: Loss of activity.

Sites not aligning to the query:

78 E→Q: No effect on the activity.
145 E→Q: Loss of activity.

5d7zA Crystal structure of glyoxalase i from zea mays (see paper)
35% identity, 93% coverage: 4:126/132 of query aligns to 141:267/281 of 5d7zA

query
sites
5d7zA

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D

Sites not aligning to the query:

active site: 13, 64, 82, 130
binding nickel (ii) ion: 276, 281

3w0tA Human glyoxalase i with an n-hydroxypyridone derivative inhibitor
32% identity, 92% coverage: 4:125/132 of query aligns to 24:167/176 of 3w0tA

query
sites
3w0tA

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active site: Q26 (≠ H6), E92 (= E53), H119 (= H72), E165 (= E123)
binding N-[3-(1-hydroxy-6-oxo-4-phenyl-1,6-dihydropyridin-2-yl)phenyl]methanesulfonamide: Q26 (≠ H6), F55 (= F35), F60 (= F38), L62 (= L40), F64 (≠ Y42), E92 (= E53), H119 (= H72), E165 (= E123)
binding zinc ion: Q26 (≠ H6), E92 (= E53), H119 (= H72), E165 (= E123)

Sites not aligning to the query:

binding N-[3-(1-hydroxy-6-oxo-4-phenyl-1,6-dihydropyridin-2-yl)phenyl]methanesulfonamide: 172, 176

3vw9A Human glyoxalase i with an n-hydroxypyridone inhibitor (see paper)
32% identity, 92% coverage: 4:125/132 of query aligns to 24:167/176 of 3vw9A

query
sites
3vw9A

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active site: Q26 (≠ H6), E92 (= E53), H119 (= H72), E165 (= E123)
binding 1-hydroxy-6-[1-(3-methoxypropyl)-1H-pyrrolo[2,3-b]pyridin-5-yl]-4-phenylpyridin-2(1H)-one: Q26 (≠ H6), C53 (≠ L33), L62 (= L40), E92 (= E53), H119 (= H72), M150 (≠ L108), F155 (= F113), E165 (= E123)
binding zinc ion: Q26 (≠ H6), E92 (= E53), H119 (= H72), E165 (= E123)

Sites not aligning to the query:

binding 1-hydroxy-6-[1-(3-methoxypropyl)-1H-pyrrolo[2,3-b]pyridin-5-yl]-4-phenylpyridin-2(1H)-one: 172, 176

1qipA Human glyoxalase i complexed with s-p- nitrobenzyloxycarbonylglutathione (see paper)
32% identity, 92% coverage: 4:125/132 of query aligns to 24:167/176 of 1qipA

query
sites
1qipA

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L

L

active site: Q26 (≠ H6), E92 (= E53), H119 (= H72), E165 (= E123)
binding s-p-nitrobenzyloxycarbonylglutathione: R30 (= R10), Q51 (≠ H31), C53 (≠ L33), F60 (= F38), F64 (≠ Y42), I81 (= I52), L85 (vs. gap), T94 (= T55), N96 (= N57), R115 (vs. gap), M150 (≠ L108), F155 (= F113)
binding zinc ion: Q26 (≠ H6), E92 (= E53), H119 (= H72), E165 (= E123)

Sites not aligning to the query:

binding s-p-nitrobenzyloxycarbonylglutathione: 172

1qinA Human glyoxalase i complexed with s-(n-hydroxy-n-p- iodophenylcarbamoyl) glutathione (see paper)
32% identity, 92% coverage: 4:125/132 of query aligns to 24:167/176 of 1qinA

query
sites
1qinA

L

L

I

Q

H
x
Q

T

T

M

M

I

L

R
|
R

V

V

Q

K

D

D

L

P

P

K

R

K

S

S

L

L

A

D

F

F

Y

Y

Q

T

K

R

A

V

F

L

N

G

F

M

D

T

V

L

S

I

H

Q

R

K

L

C

D

D

F

F

P

P

-

I

-

M

D

K

F
|
F

T

S

L
|
L

A

Y

Y
x
F

L

L

-

A

-

Y

-

E

-

D

R

K

N

N

-

D

-

I

-

P

A

K

E

E

A

K

D

D

T

E

E

K

I

I

-

A

-

W

-

A

-

L

-

S

-

R

-

K

-

A

-

T

-

L

E
|
E

L

L

T
|
T

W

H

N
|
N

K

W

G

G

R

T

E

E

-

D

-

E

-

T

E

Q

P

S

Y

Y

S

H

H

N

G

G

D

N

-

S

-

D

-

P

-
x
R

G

G

Y

F

G

G

H
|
H

V

I

A

G

F

I

V

A

V

V

D

P

D

D

A

V

K

Y

H

S

E

A

R

C

Q

K

R

R

L

F

L

E

D

E

L

L

G

G

M

V

-

K

-

F

-

V

-
x
K

T

K

P

P

N

D

D

D

L
x
G

R
x
K

E

-

F

-

H

-

N

-

D

-

N

-

G

-

E

-

L

-

L
x
M

A

K

R

G

Y

L

F

A

F

F

I

I

Q

Q

D

D

P

P

D

D

G

G

Y

Y

K

W

I

I

E
|
E

V

I

L

L

active site: Q26 (≠ H6), E92 (= E53), H119 (= H72), E165 (= E123)
binding s-(n-hydroxy-n-iodophenylcarbamoyl)glutathione: Q26 (≠ H6), R30 (= R10), F60 (= F38), L62 (= L40), F64 (≠ Y42), E92 (= E53), T94 (= T55), N96 (= N57), R115 (vs. gap), H119 (= H72), K143 (vs. gap), G148 (≠ L97), K149 (≠ R98), M150 (≠ L108), E165 (= E123)
binding zinc ion: Q26 (≠ H6), E92 (= E53)

Sites not aligning to the query:

binding s-(n-hydroxy-n-iodophenylcarbamoyl)glutathione: 172

1froA Human glyoxalase i with benzyl-glutathione inhibitor (see paper)
32% identity, 92% coverage: 4:125/132 of query aligns to 24:167/176 of 1froA

query
sites
1froA

L

L

I

Q

H
x
Q

T

T

M

M

I

L

R
|
R

V

V

Q

K

D

D

L

P

P

K

R

K

S

S

L

L

A

D

F

F

Y

Y

Q

T

K

R

A

V

F

L

N

G

F

M

D

T

V

L

S

I

H

Q

R

K

L

C

D

D

F

F

P

P

-

I

-

M

D

K

F
|
F

T

S

L

L

A

Y

Y

F

L

L

-

A

-

Y

-

E

-

D

R

K

N

N

-

D

-

I

-

P

A

K

E

E

A

K

D

D

T

E

E

K

I

I

-

A

-

W

-

A

-

L

-

S

-

R

-

K

-

A

-

T

-

L

E
|
E

L

L

T
|
T

W

H

N
|
N

K

W

G

G

R

T

E

E

-

D

-

E

-

T

E

Q

P

S

Y

Y

S

H

H

N

G

G

D

N

-

S

-

D

-

P

-
x
R

G

G

Y

F

G

G

H
|
H

V

I

A

G

F

I

V

A

V

V

D

P

D

D

A

V

K

Y

H

S

E

A

R

C

Q

K

R

R

L

F

L

E

D

E

L

L

G

G

M

V

-

K

-

F

-

V

-

K

T

K

P

P

N

D

D

D

L

G

R

K

E

-

F

-

H

-

N

-

D

-

N

-

G

-

E

-

L

-

L
x
M

A

K

R

G

Y

L

F

A

F
|
F

I

I

Q

Q

D

D

P

P

D

D

G

G

Y

Y

K

W

I

I

E
|
E

V

I

L

L

active site: Q26 (≠ H6), E92 (= E53), H119 (= H72), E165 (= E123)
binding s-benzyl-glutathione: R30 (= R10), F60 (= F38), T94 (= T55), N96 (= N57), R115 (vs. gap), M150 (≠ L108), F155 (= F113), E165 (= E123)
binding zinc ion: Q26 (≠ H6), E92 (= E53), H119 (= H72), E165 (= E123)

Sites not aligning to the query:

binding s-benzyl-glutathione: 176

Q04760 Lactoylglutathione lyase; Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase; EC 4.4.1.5 from Homo sapiens (Human) (see 12 papers)
33% identity, 92% coverage: 4:125/132 of query aligns to 32:175/184 of Q04760

query
sites
Q04760

L

L

I

Q

H
x
Q

T

T

M

M

I

L

R

R

V

V

Q

K

D

D

L

P

P

K

R

K

S
|
S

L

L

A

D

F

F

Y

Y

Q

T

K

R

A

V

F

L

N

G

F

M

D

T

V

L

S

I

H

Q

R

K

L
x
C

D

D

F

F

P

P

-

I

-

M

D

K

F

F

T
x
S

L

L

A

Y

Y

F

L

L

-

A

-

Y

-

E

-

D

R

K

N

N

-

D

-

I

-

P

A

K

E

E

A

K

D

D

T

E

E

K

I

I

-

A

-

W

-

A

-

L

-
x
S

-

R

-

K

-

A

-
x
T

-

L

E
|
E

L

L

T
|
T

W

H

N

N

K

W

G

G

R
x
T

E

E

-

D

-
x
E

-

T

E

Q

P

S

Y

Y

S

H

H

N

G

G

D

N

-

S

-

D

-

P

-

R

G

G

Y

F

G

G

H
|
H

V

I

A

G

F

I

V

A

V

V

D

P

D

D

A

V

K

Y

H

S

E

A

R
x
C

Q

K

R

R

L

F

L

E

D

E

L

L

G

G

M

V

T

-

P

-

N

-

D

-

L

-

R

-

E

K

F

F

-

V

-

K

H

K

N

P

D

D

N

D

G

G

E

K

L

M

L

K

A

G

R

-

Y

L

F

A

F

F

I

I

Q

Q

D

D

P

P

D

D

G

G

Y

Y

K

W

I

I

E
|
E

V

I

L

L

Q34 (≠ H6) binding ; mutation to E: Reduces enzyme activity by 99%.
S45 (= S17) mutation to A: No effect on phosphorylation.
C61 (≠ L33) modified: Disulfide link with 139, Alternate; mutation to A: No effect on NO-mediated modification.
S69 (≠ T39) mutation to A: No effect on phosphorylation.
S94 (vs. gap) mutation to A: No effect on phosphorylation.
T98 (vs. gap) mutation to A: No effect on phosphorylation.
E100 (= E53) binding ; mutation to Q: Reduces enzyme activity by over 99%.
T102 (= T55) mutation to A: No effect on phosphorylation.
T107 (≠ R60) modified: Phosphothreonine; mutation to A: Loss of phosphorylation.
E111 (vs. gap) to A: in dbSNP:rs4746
H127 (= H72) binding in other chain
C139 (≠ R84) modified: S-glutathionyl cysteine; alternate; modified: Disulfide link with 61, Alternate; mutation to A: Impaired NO-mediated modification. Loss of NO-mediated modification; when associated with A-19 or A-20.
E173 (= E123) active site, Proton donor/acceptor; binding in other chain; mutation to Q: Abolishes enzyme activity.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine
19 modified: Disulfide link with 20; C → Y: in dbSNP:rs17855424; C→A: No effect on NO-mediated modification. Impaired NO-mediated modification; when associated with A-20. Loss of NO-mediated modification; when associated with A-139.
20 modified: Disulfide link with 19; C→A: No effect on NO-mediated modification. Impaired NO-mediated modification; when associated with A-19. Loss of NO-mediated modification; when associated with A-139.

3w0uA Human glyoxalase i with an n-hydroxypyridone inhibitor
32% identity, 92% coverage: 4:125/132 of query aligns to 24:167/175 of 3w0uA

query
sites
3w0uA

L

L

I

Q

H
x
Q

T

T

M

M

I

L

R

R

V

V

Q

K

D

D

L

P

P

K

R

K

S

S

L

L

A

D

F

F

Y

Y

Q

T

K

R

A

V

F

L

N

G

F

M

D

T

V

L

S

I

H

Q

R

K

L

C

D

D

F

F

P

P

-

I

-

M

D

K

F
|
F

T

S

L
|
L

A

Y

Y

F

L

L

-

A

-

Y

-

E

-

D

R

K

N

N

-

D

-

I

-

P

A

K

E

E

A

K

D

D

T

E

E

K

I

I

-

A

-

W

-

A

-

L

-

S

-

R

-

K

-

A

-

T

-

L

E
|
E

L

L

T

T

W

H

N

N

K

W

G

G

R

T

E

E

-

D

-

E

-

T

E

Q

P

S

Y

Y

S

H

H

N

G

G

D

N

-

S

-

D

-

P

-

R

G

G

Y

F

G

G

H
|
H

V

I

A

G

F

I

V

A

V

V

D

P

D

D

A

V

K

Y

H

S

E

A

R

C

Q

K

R

R

L

F

L

E

D

E

L

L

G

G

M

V

-

K

-

F

-

V

-
x
K

T

K

P

P

N

D

D

D

L

G

R

K

E

-

F

-

H

-

N

-

D

-

N

-

G

-

E

-

L

-

L
x
M

A

K

R

G

Y

L

F

A

F

F

I

I

Q

Q

D

D

P

P

D

D

G

G

Y

Y

K

W

I

I

E
|
E

V

I

L

L

active site: Q26 (≠ H6), E92 (= E53), H119 (= H72), E165 (= E123)
binding N-[3-(1-Hydroxy-6-oxo-4-phenyl-1,6-dihydro-pyridin-2-yl)-5-methanesulfonylamino-phenyl]-methanesulfonamide: Q26 (≠ H6), F60 (= F38), L62 (= L40), E92 (= E53), H119 (= H72), K143 (vs. gap), M150 (≠ L108), E165 (= E123)
binding zinc ion: Q26 (≠ H6), E92 (= E53), H119 (= H72), E165 (= E123)

Sites not aligning to the query:

binding N-[3-(1-Hydroxy-6-oxo-4-phenyl-1,6-dihydro-pyridin-2-yl)-5-methanesulfonylamino-phenyl]-methanesulfonamide: 172

7wt0A Human glyoxalase i (with c-ter his tag) in complex with tlsc702 (see paper)
33% identity, 92% coverage: 4:125/132 of query aligns to 31:174/185 of 7wt0A

query
sites
7wt0A

L

L

I

Q

H
x
Q

T

T

M

M

I

L

R

R

V

V

Q

K

D

D

L

P

P

K

R

K

S

S

L

L

A

D

F

F

Y

Y

Q

T

K

R

A

V

F

L

N

G

F

M

D

T

V

L

S

I

H

Q

R

K

L
x
C

D

D

F
|
F

P

P

-

I

-

M

D

K

F

F

T

S

L
|
L

A

Y

Y
x
F

L

L

-

A

-

Y

-

E

-

D

R

K

N

N

-

D

-

I

-

P

A

K

E

E

A

K

D

D

T

E

E

K

I

I

-

A

-

W

-

A

-
x
L

-

S

-

R

-

K

-

A

-

T

-

L

E
|
E

L

L

T

T

W

H

N

N

K

W

G

G

R

T

E

E

-

D

-

E

-

T

E

Q

P

S

Y

Y

S

H

H

N

G

G

D

N

-

S

-

D

-

P

-

R

G

G

Y

F

G

G

H
|
H

V

I

A

G

F

I

V

A

V

V

D

P

D

D

A

V

K

Y

H

S

E

A

R

C

Q

K

R

R

L

F

L

E

D

E

L

L

G

G

M

V

T

-

P

-

N

-

D

-

L

-

R

-

E

K

F

F

-

V

-
x
K

H

K

N

P

D

D

N

D

G

G

E

K

L
x
M

L

K

A

G

R

-

Y
x
L

F

A

F
|
F

I

I

Q

Q

D

D

P

P

D

D

G

G

Y

Y

K

W

I

I

E
|
E

V

I

L

L

binding (~{E})-3-(1,3-benzothiazol-2-yl)-4-(4-methoxyphenyl)but-3-enoic acid: C60 (≠ L33), F62 (= F35), L69 (= L40), F71 (≠ Y42), L92 (vs. gap), E99 (= E53), H126 (= H72), K150 (vs. gap), M157 (≠ L107), L160 (≠ Y111), F162 (= F113), E172 (= E123)
binding zinc ion: Q33 (≠ H6), E99 (= E53), H126 (= H72), E172 (= E123)

Sites not aligning to the query:

binding (~{E})-3-(1,3-benzothiazol-2-yl)-4-(4-methoxyphenyl)but-3-enoic acid: 179, 183

7wszA Human glyoxalase i (with c-ter his tag) in glycerol-bound form (see paper)
32% identity, 92% coverage: 4:125/132 of query aligns to 24:167/183 of 7wszA

query
sites
7wszA

L

L

I

Q

H
x
Q

T

T

M

M

I

L

R

R

V

V

Q

K

D

D

L

P

P

K

R

K

S

S

L

L

A

D

F

F

Y

Y

Q

T

K

R

A

V

F

L

N

G

F

M

D

T

V

L

S
x
I

H
x
Q

R
x
K

L

C

D

D

F

F

P

P

-

I

-

M

D

K

F

F

T

S

L

L

A

Y

Y

F

L

L

-

A

-

Y

-

E

-

D

R

K

N

N

-

D

-

I

-

P

A

K

E

E

A

K

D

D

T

E

E
x
K

I

I

-

A

-

W

-

A

-

L

-

S

-

R

-

K

-

A

-

T

-

L

E
|
E

L

L

T

T

W

H

N

N

K

W

G

G

R

T

E

E

-

D

-

E

-

T

E

Q

P

S

Y

Y

S

H

H

N

G

G

D

N

-

S

-

D

-

P

-

R

G

G

Y

F

G

G

H
|
H

V

I

A

G

F

I

V

A

V

V

D

P

D

D

A

V

K

Y

H

S

E

A

R

C

Q

K

R

R

L

F

L

E

D

E

L

L

G

G

M

V

-

K

-

F

-

V

-

K

T

K

P

P

N

D

D

D

L

G

R

K

E

-

F

-

H

-

N

-

D

-

N

-

G

-

E

-

L

-

L
x
M

A

K

R

G

Y

L

F

A

F

F

I

I

Q

Q

D

D

P

P

D

D

G

G

Y

Y

K

W

I

I

E
|
E

V

I

L

L

binding glycerol: I50 (≠ S30), Q51 (≠ H31), K52 (≠ R32), K80 (≠ E51), H119 (= H72), M150 (≠ L108), E165 (= E123)
binding zinc ion: Q26 (≠ H6), E92 (= E53), H119 (= H72), E165 (= E123)

Sites not aligning to the query:

binding glycerol: 176

Q9CPU0 Lactoylglutathione lyase; Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase; EC 4.4.1.5 from Mus musculus (Mouse) (see paper)
30% identity, 92% coverage: 4:125/132 of query aligns to 32:175/184 of Q9CPU0

query
sites
Q9CPU0

L

L

I

Q

H
x
Q

T

T

M

M

I

L

R

R

V

I

Q

K

D

D

L

P

P

K

R

K

S

S

L

L

A

D

F

F

Y

Y

Q

T

K

R

A

V

F

L

N

G

F

L

D

T

V

L

S

L

H

Q

R

K

L

L

D

D

F

F

P

P

-

A

-

M

D

K

F

F

T

S

L

L

A

Y

Y

F

L

L

R

A

N

Y

A

E

E

D

A

K

D

N

-

D

-

I

-

P

-

K

-

D

-

K

-

S

-

E

-

K

-

T

-

A

-

W

-

T

-

F

-

S

-

R

-

K

T

A

E

T

I

L

E
|
E

L

L

T

T

W

H

N

N

K

W

G

G

R

T

E

E

-

D

-

E

-

T

E

Q

P

S

Y

Y

S

H

H

N

G

G

D

N

-

S

-

D

-

P

-

R

G

G

Y

F

G

G

H
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H

V

I

A

G

F

I

V

A

V

V

D

P

D

D

A

V

K

Y

H

S

E

A

R

C

Q

K

R

R

L

F

L

E

D

E

L

L

G

G

M

V

T

-

P

-

N

-

D

-

L

-

R

-

E

K

F

F

-

V

-

K

H

K

N

P

D

D

N

D

G

G

E

K

L

M

L

K

A

G

R

-

Y

L

F

A

F

F

I

I

Q

Q

D

D

P

P

D

D

G

G

Y

Y

K

W

I

I

E
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E

V

I

L

L

Q34 (≠ H6) binding
E100 (= E53) binding
H127 (= H72) binding in other chain
E173 (= E123) binding in other chain

4opnA Crystal structure of mouse glyoxalase i complexed with mah
30% identity, 92% coverage: 4:125/132 of query aligns to 24:167/172 of 4opnA

query
sites
4opnA

L

L

I

Q

H
x
Q

T

T

M

M

I

L

R
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R

V

I

Q

K

D

D

L

P

P

K

R

K

S

S

L

L

A

D

F

F

Y

Y

Q

T

K

R

A

V

F

L

N

G

F

L

D

T

V

L

S

L

H

Q

R

K

L

L

D

D

F

F

P

P

-

A

-

M

D

K

F
|
F

T

S

L

L

A

Y

Y

F

L

L

R

A

N

Y

A

E

E

D

A

K

D

N

-

D

-

I

-

P

-

K

-

D

-

K

-

S

-

E

-

K

-

T

-

A

-

W

-

T

-
x
F

-

S

-

R

-

K

T

A

E

T

I

L

E
|
E

L

L

T
|
T

W

H

N
|
N

K

W

G

G

R

T

E

E

-

D

-

E

-

T

E

Q

P

S

Y

Y

S

H

H

N

G

G

D

N

-

S

-

D

-

P

-
x
R

G

G

Y

F

G

G

H
|
H

V

I

A

G

F

I

V

A

V

V

D

P

D

D

A

V

K

Y

H

S

E

A

R

C

Q

K

R

R

L

F

L

E

D

E

L

L

G

G

M

V

T

-

P

-

N

-

D

-

L

-

R

-

E

K

F

F

-

V

-
x
K

H

K

N

P

D

D

N

D

G

G

E
x
K

L
x
M

L

K

A

G

R

-

Y
x
L

F

A

F

F

I

I

Q

Q

D

D

P

P

D

D

G

G

Y

Y

K

W

I

I

E
|
E

V

I

L

L

active site: Q26 (≠ H6), E92 (= E53), H119 (= H72), E165 (= E123)
binding L-gamma-glutamyl-N-(3-ethynylphenyl)-N-hydroxy-L-glutaminylglycine: Q26 (≠ H6), R30 (= R10), F60 (= F38), F85 (vs. gap), E92 (= E53), T94 (= T55), N96 (= N57), R115 (vs. gap), H119 (= H72), K143 (vs. gap), K149 (≠ E106), M150 (≠ L107), L153 (≠ Y111), E165 (= E123)
binding zinc ion: Q26 (≠ H6), E92 (= E53)

Sites not aligning to the query:

binding L-gamma-glutamyl-N-(3-ethynylphenyl)-N-hydroxy-L-glutaminylglycine: 172

4x2aA Crystal structure of mouse glyoxalase i complexed with baicalein (see paper)
30% identity, 92% coverage: 4:125/132 of query aligns to 18:161/167 of 4x2aA

query
sites
4x2aA

L

L

I

Q

H
x
Q

T

T

M

M

I

L

R

R

V

I

Q

K

D

D

L

P

P

K

R

K

S

S

L

L

A

D

F

F

Y

Y

Q

T

K

R

A

V

F

L

N

G

F

L

D

T

V

L

S

L

H

Q

R

K

L

L

D

D

F
|
F

P

P

-

A

-

M

D

K

F

F

T

S

L
|
L

A

Y

Y

F

L

L

R

A

N

Y

A

E

E

D

A

K

D

N

-

D

-

I

-

P

-

K

-

D

-

K

-

S

-

E

-

K

-

T

-

A

-

W

-

T

-
x
F

-

S

-

R

-

K

T

A

E

T

I

L

E
|
E

L

L

T

T

W

H

N

N

K

W

G

G

R

T

E

E

-

D

-

E

-

T

E

Q

P

S

Y

Y

S

H

H

N

G

G

D

N

-

S

-

D

-

P

-

R

G

G

Y

F

G

G

H
|
H

V

I

A

G

F

I

V

A

V

V

D

P

D

D

A

V

K

Y

H

S

E

A

R

C

Q

K

R

R

L

F

L

E

D

E

L

L

G

G

M

V

T

-

P

-

N

-

D

-

L

-

R

-

E

K

F

F

-

V

-

K

H

K

N

P

D

D

N

D

G

G

E

K

L
x
M

L

K

A

G

R

-

Y

L

F

A

F

F

I

I

Q

Q

D

D

P

P

D

D

G

G

Y

Y

K

W

I

I

E
|
E

V

I

L

L

active site: Q20 (≠ H6), E86 (= E53), H113 (= H72), E159 (= E123)
binding 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one: Q20 (≠ H6), F49 (= F35), L56 (= L40), F79 (vs. gap), E86 (= E53), H113 (= H72), M144 (≠ L107), E159 (= E123)
binding zinc ion: Q20 (≠ H6), E86 (= E53), H113 (= H72), E159 (= E123)

Sites not aligning to the query:

binding 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one: 166, 167

Query Sequence

>BPHYT_RS13485 FitnessBrowser__BFirm:BPHYT_RS13485
MAKLIHTMIRVQDLPRSLAFYQKAFNFDVSHRLDFPDFTLAYLRNAEADTEIELTWNKGR
EEPYSHGDGYGHVAFVVDDAKHERQRLLDLGMTPNDLREFHNDNGELLARYFFIQDPDGY
KIEVLERHGHYQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory