SitesBLAST
Comparing BPHYT_RS13550 FitnessBrowser__BFirm:BPHYT_RS13550 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
I6YCA3 Acyl-CoA dehydrogenase FadE26; ACAD; 3-oxocholest-4-en-26-oyl-CoA dehydrogenase alpha subunit; EC 1.3.99.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
33% identity, 100% coverage: 1:402/402 of query aligns to 1:395/400 of I6YCA3
- IGYS 127:130 (≠ QGYS 126:129) binding
- T136 (≠ S135) binding
- S162 (≠ T165) binding
- E247 (= E248) mutation to A: Loss of dehydrogenase activity.
- TNE 380:382 (≠ SNE 387:389) binding
4x28A Crystal structure of the chse4-chse5 complex from mycobacterium tuberculosis (see paper)
34% identity, 100% coverage: 1:402/402 of query aligns to 1:382/386 of 4x28A
- active site: Y122 (= Y128), S123 (= S129), E240 (= E248), G365 (= G385), M377 (≠ K397)
- binding dihydroflavine-adenine dinucleotide: I120 (≠ Q126), Y122 (= Y128), S123 (= S129), G128 (= G134), T129 (≠ S135), W153 (= W163), S155 (≠ T165), F363 (≠ I383), T367 (≠ S387), E369 (= E389), V370 (≠ I390)
P71858 Acyl-CoA dehydrogenase FadE29; ACAD; 3-oxo-23,24-bisnorchol-4-en-22-oyl-CoA dehydrogenase beta subunit; 3-oxo-4-pregnene-20-carboxyl-CoA dehydrogenase beta subunit; EC 1.3.99.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
33% identity, 99% coverage: 1:396/402 of query aligns to 1:376/387 of P71858
- E241 (= E248) mutation to Q: Unable to dehydrogenate pregnene-carboxyl-CoA ester.
6wy8B Tcur3481-tcur3483 steroid acad (see paper)
31% identity, 100% coverage: 1:402/402 of query aligns to 1:381/384 of 6wy8B
- active site: Y126 (= Y128), T127 (≠ S129), E241 (= E248), T376 (≠ K397)
- binding flavin-adenine dinucleotide: I124 (≠ Q126), Y126 (= Y128), T127 (≠ S129), G132 (= G134), T133 (≠ S135), F157 (≠ W163), S159 (≠ T165), V359 (≠ K380), F362 (≠ I383), G363 (≠ F384), V366 (≠ S387), E368 (= E389)
6wy9A Tcur3481-tcur3483 steroid acad g363a variant (see paper)
31% identity, 97% coverage: 12:402/402 of query aligns to 8:377/380 of 6wy9A
- active site: Y122 (= Y128), T123 (≠ S129), E237 (= E248), T372 (≠ K397)
- binding dihydroflavine-adenine dinucleotide: I120 (≠ Q126), Y122 (= Y128), T123 (≠ S129), G128 (= G134), T129 (≠ S135), F153 (≠ W163), S155 (≠ T165), F358 (≠ I383), V362 (≠ S387), E364 (= E389)
P96855 Acyl-CoA dehydrogenase FadE34; ACAD; 3-oxochol-4-en-24-oyl-CoA dehydrogenase; EC 1.3.99.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
33% identity, 88% coverage: 50:402/402 of query aligns to 388:709/711 of P96855
- E581 (= E248) mutation to Q: Displays less than 1% activity with cholyl-CoA as substrate. Still binds FAD.
Sites not aligning to the query:
- 236 R→A: Displays less than 2% activity with cholyl-CoA as substrate. Cannot bind FAD.
2pg0A Crystal structure of acyl-coa dehydrogenase from geobacillus kaustophilus
30% identity, 88% coverage: 48:399/402 of query aligns to 43:378/380 of 2pg0A
- active site: M124 (≠ Y128), T125 (≠ S129), E243 (= E248), A364 (≠ G385), R376 (≠ K397)
- binding flavin-adenine dinucleotide: I122 (≠ Q126), M124 (≠ Y128), T125 (≠ S129), G130 (= G134), S131 (= S135), F155 (≠ W163), I156 (≠ T164), T157 (= T165), R269 (= R273), F272 (vs. gap), F279 (≠ D282), Q337 (≠ A353), L338 (= L354), G340 (≠ D356), G341 (= G357), V359 (≠ K380), I362 (= I383), Y363 (≠ F384), T366 (≠ S387), E368 (= E389), M369 (≠ I390)
6fahD Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
27% identity, 99% coverage: 3:400/402 of query aligns to 1:377/379 of 6fahD
- active site: L124 (≠ Y128), T125 (≠ S129), G241 (≠ E248), G374 (≠ K397)
- binding flavin-adenine dinucleotide: F122 (≠ Q126), L124 (≠ Y128), T125 (≠ S129), R152 (≠ Q160), F155 (≠ W163), T157 (= T165), E198 (= E212), R267 (≠ Q272), Q269 (≠ R274), F270 (≠ N275), I274 (≠ L279), F277 (≠ D282), Q335 (≠ R340), I336 (≠ A341), G339 (≠ A344), Y361 (≠ F384), T364 (≠ S387), Q366 (≠ E389)
8hk0B Crystal structure of fic32-33 complex from streptomyces ficellus nrrl 8067 (see paper)
31% identity, 63% coverage: 1:252/402 of query aligns to 1:247/379 of 8hk0B
Sites not aligning to the query:
1ukwB Crystal structure of medium-chain acyl-coa dehydrogenase from thermus thermophilus hb8
29% identity, 100% coverage: 1:401/402 of query aligns to 1:378/379 of 1ukwB
- active site: L124 (≠ Y128), S125 (= S129), T241 (≠ E248), E362 (≠ G385), R374 (≠ K397)
- binding cobalt (ii) ion: D145 (≠ E153), H146 (= H154)
- binding flavin-adenine dinucleotide: F122 (≠ Q126), L124 (≠ Y128), S125 (= S129), G130 (= G134), S131 (= S135), W155 (= W163), S157 (≠ T165), K200 (≠ L209), L357 (≠ K380), Y361 (≠ F384), E362 (≠ G385), T364 (≠ S387), E366 (= E389), L370 (≠ N393)
1ukwA Crystal structure of medium-chain acyl-coa dehydrogenase from thermus thermophilus hb8
29% identity, 100% coverage: 1:401/402 of query aligns to 1:378/379 of 1ukwA
- active site: L124 (≠ Y128), S125 (= S129), T241 (≠ E248), E362 (≠ G385), R374 (≠ K397)
- binding flavin-adenine dinucleotide: F122 (≠ Q126), L124 (≠ Y128), S125 (= S129), G130 (= G134), S131 (= S135), W155 (= W163), S157 (≠ T165), L357 (≠ K380), Y361 (≠ F384), E362 (≠ G385), T364 (≠ S387), E366 (= E389), L370 (≠ N393)
3r7kA Crystal structure of a probable acyl coa dehydrogenase from mycobacterium abscessus atcc 19977 / dsm 44196 (see paper)
29% identity, 98% coverage: 6:399/402 of query aligns to 6:377/378 of 3r7kA
- active site: V126 (≠ Y128), T127 (≠ S129), E242 (= E248), G363 (= G385), K375 (= K397)
- binding dihydroflavine-adenine dinucleotide: V126 (≠ Y128), T127 (≠ S129), G132 (= G134), S133 (= S135), F157 (≠ W163), I158 (≠ T164), T159 (= T165), R268 (≠ Q272), T270 (≠ R274), F271 (≠ N275), L275 (= L279), R278 (≠ D282), I281 (≠ F285), Q336 (≠ D356), I337 (≠ G357), G340 (= G360), I358 (≠ K380), T365 (≠ S387), E367 (= E389)
1egcA Structure of t255e, e376g mutant of human medium chain acyl-coa dehydrogenase complexed with octanoyl-coa (see paper)
25% identity, 98% coverage: 3:397/402 of query aligns to 5:379/387 of 1egcA
- active site: V126 (≠ Y128), T127 (≠ S129), E246 (= E248), G367 (= G385), R379 (≠ K397)
- binding octanoyl-coenzyme a: E90 (≠ G93), L94 (= L97), Y124 (≠ Q126), S133 (= S135), V135 (≠ L137), N182 (≠ G188), F236 (≠ W238), M240 (≠ K242), F243 (≠ L245), D244 (≠ T246), R247 (= R249), Y366 (≠ F384), G367 (= G385), G368 (= G386)
- binding flavin-adenine dinucleotide: Y124 (≠ Q126), V126 (≠ Y128), T127 (≠ S129), G132 (= G134), S133 (= S135), W157 (= W163), T159 (= T165), R272 (≠ Q272), T274 (≠ R274), F275 (≠ N275), L279 (= L279), H282 (≠ D282), I285 (≠ F285), Q340 (≠ R340), I341 (≠ A341), G344 (≠ A344), I362 (≠ L374), I365 (= I383), Y366 (≠ F384), T369 (≠ S387), Q371 (≠ E389)
3mdeA Crystal structures of medium chain acyl-coa dehydrogenase from pig liver mitochondria with and without substrate (see paper)
25% identity, 100% coverage: 1:401/402 of query aligns to 2:382/385 of 3mdeA
- active site: V125 (≠ Y128), T126 (≠ S129), T245 (≠ E248), E366 (≠ G385), R378 (≠ K397)
- binding octanoyl-coenzyme a: T86 (≠ V90), E89 (≠ G93), L93 (= L97), S132 (= S135), V134 (≠ L137), S181 (≠ G188), F235 (≠ W238), M239 (≠ K242), F242 (≠ L245), R314 (≠ V315), Y365 (≠ F384), E366 (≠ G385), G367 (= G386)
- binding flavin-adenine dinucleotide: Y123 (≠ Q126), V125 (≠ Y128), T126 (≠ S129), G131 (= G134), S132 (= S135), W156 (= W163), I157 (≠ T164), T158 (= T165), R271 (≠ Q272), T273 (≠ R274), F274 (≠ N275), L278 (= L279), H281 (≠ D282), Q339 (≠ R340), V340 (≠ A341), G343 (≠ A344), I361 (≠ L374), T368 (≠ S387), Q370 (≠ E389)
3mddA Crystal structures of medium chain acyl-coa dehydrogenase from pig liver mitochondria with and without substrate (see paper)
25% identity, 100% coverage: 1:401/402 of query aligns to 2:382/385 of 3mddA
- active site: V125 (≠ Y128), T126 (≠ S129), T245 (≠ E248), E366 (≠ G385), R378 (≠ K397)
- binding flavin-adenine dinucleotide: Y123 (≠ Q126), T126 (≠ S129), G131 (= G134), S132 (= S135), W156 (= W163), T158 (= T165), R271 (≠ Q272), T273 (≠ R274), F274 (≠ N275), H281 (≠ D282), Q339 (≠ R340), V340 (≠ A341), G343 (≠ A344), I361 (≠ L374), T368 (≠ S387), Q370 (≠ E389)
1udyA Medium-chain acyl-coa dehydrogenase with 3-thiaoctanoyl-coa (see paper)
25% identity, 100% coverage: 1:401/402 of query aligns to 2:382/385 of 1udyA
- active site: V125 (≠ Y128), T126 (≠ S129), T245 (≠ E248), E366 (≠ G385), R378 (≠ K397)
- binding 3-thiaoctanoyl-coenzyme a: L93 (= L97), Y123 (≠ Q126), S132 (= S135), S181 (≠ G188), F235 (≠ W238), M239 (≠ K242), F242 (≠ L245), V249 (≠ I252), R314 (≠ V315), Y365 (≠ F384), E366 (≠ G385), G367 (= G386), I371 (= I390), I375 (= I394)
- binding flavin-adenine dinucleotide: Y123 (≠ Q126), T126 (≠ S129), G131 (= G134), S132 (= S135), W156 (= W163), T158 (= T165), T273 (≠ R274), F274 (≠ N275), Q339 (≠ R340), V340 (≠ A341), G343 (≠ A344), T368 (≠ S387), Q370 (≠ E389)
P41367 Medium-chain specific acyl-CoA dehydrogenase, mitochondrial; MCAD; EC 1.3.8.7 from Sus scrofa (Pig) (see 2 papers)
25% identity, 100% coverage: 1:401/402 of query aligns to 37:417/421 of P41367
- 158:167 (vs. 126:135, 60% identical) binding in other chain
- S167 (= S135) binding
- WIT 191:193 (≠ WTT 163:165) binding in other chain
- S216 (≠ G188) binding
- D278 (≠ T246) binding
- R281 (= R249) binding
- RKT 306:308 (≠ QRR 272:274) binding
- HQ 316:317 (≠ DP 282:283) binding in other chain
- R349 (≠ V315) binding
- T351 (≠ G317) binding
- QVFGG 374:378 (≠ RAMGA 340:344) binding
- E401 (≠ G385) active site, Proton acceptor; binding
- GTAQ 402:405 (≠ GSNE 386:389) binding in other chain
4iv6B X-ray crystal structure of an isovaleryl-coa dehydrogenase from mycobacterium smegmatis (see paper)
28% identity, 77% coverage: 93:400/402 of query aligns to 86:376/383 of 4iv6B
- active site: L121 (≠ Y128), T122 (≠ S129), G240 (≠ E248), E361 (vs. gap), K373 (= K397)
- binding dihydroflavine-adenine dinucleotide: L121 (≠ Y128), T122 (≠ S129), G126 (≠ A133), G127 (= G134), S128 (= S135), W152 (= W163), I153 (≠ T164), S154 (≠ T165), R266 (≠ Q272), S268 (≠ R274), F269 (≠ N275), I273 (≠ L279), H276 (≠ D282), V279 (≠ F285), R334 (= R340), V335 (≠ A341), G338 (≠ A344), L356 (= L381), G360 (= G385), T363 (≠ S387), E365 (= E389), I366 (= I390)
1jqiA Crystal structure of rat short chain acyl-coa dehydrogenase complexed with acetoacetyl-coa (see paper)
28% identity, 86% coverage: 54:400/402 of query aligns to 51:380/384 of 1jqiA
- active site: G377 (≠ K397)
- binding acetoacetyl-coenzyme a: L95 (vs. gap), F125 (≠ Q126), S134 (= S135), F234 (≠ W238), M238 (≠ K242), Q239 (≠ Y243), L241 (= L245), D242 (≠ T246), R245 (= R249), Y364 (≠ F384), E365 (≠ G385), G366 (= G386)
- binding flavin-adenine dinucleotide: F125 (≠ Q126), L127 (≠ Y128), S128 (= S129), G133 (= G134), S134 (= S135), W158 (= W163), T160 (= T165), R270 (≠ Q272), F273 (≠ N275), L280 (≠ D282), Q338 (≠ R340), I339 (≠ A341), G342 (≠ A344), I360 (≠ K380), T367 (≠ S387), E369 (= E389), I370 (= I390)
P15651 Short-chain specific acyl-CoA dehydrogenase, mitochondrial; SCAD; Butyryl-CoA dehydrogenase; EC 1.3.8.1 from Rattus norvegicus (Rat) (see 2 papers)
28% identity, 86% coverage: 54:400/402 of query aligns to 78:407/412 of P15651
Sites not aligning to the query:
- 1:24 modified: transit peptide, Mitochondrion
Query Sequence
>BPHYT_RS13550 FitnessBrowser__BFirm:BPHYT_RS13550
MDLNFTPEEEAFRADVQAFLRDKLPQRLADKVHGGRRLTRDDMAEWHAILNAQGWLANHW
PKEYGGPGWNPVQKFIFENECALAGAPRVVPFGVNMLGPVLIKYGSEAQKRHWLPRILDG
SDWWCQGYSEPGAGSDLASVKTTAVRGVDAQGEHYLVNGQKTWTTLGHFANMIFCLVRSA
TDVRKQEGISFLLIDMKTPGVEVRPIITLDGEHEVNEVFFTDVRVPVENLVGEENKGWTC
AKYLLTYERTNIAGVGFSVAAFNRLRKIAAKQRRNGRPLAEDPAFAARMARVEIDLENMK
TTNLRVIAAVAGGGVPGAESSMLKIRGTEIRQEISSLTRRAMGAYAQPFIEEALHDGFEG
TPVGPDEAASAASLYFNNRKLSIFGGSNEIQKNIISKMILGL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory