SitesBLAST
Comparing BPHYT_RS13555 BPHYT_RS13555 acyl-CoA dehydrogenase to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P51174 Long-chain specific acyl-CoA dehydrogenase, mitochondrial; LCAD; EC 1.3.8.8 from Mus musculus (Mouse) (see paper)
33% identity, 82% coverage: 40:347/375 of query aligns to 86:399/430 of P51174
- K318 (= K265) modified: N6-acetyllysine; mutation to R: Reduces activity by 37%; reduces activity by 80% when associated with R-322.
- K322 (≠ V269) modified: N6-acetyllysine; alternate; mutation to R: Reduces activity by 23%; reduces activity by 80% when associated with R-318.
Sites not aligning to the query:
- 42 modified: N6-acetyllysine; K→R: Reduces activity by 90% when associated with R-318 and R-322.
P45954 Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial; SBCAD; 2-methyl branched chain acyl-CoA dehydrogenase; 2-MEBCAD; 2-methylbutyryl-coenzyme A dehydrogenase; 2-methylbutyryl-CoA dehydrogenase; EC 1.3.8.5 from Homo sapiens (Human) (see 6 papers)
29% identity, 92% coverage: 6:349/375 of query aligns to 58:403/432 of P45954
- V137 (= V84) mutation to L: Decreased acyl-CoA dehydrogenase activity.
- F138 (≠ E85) mutation to L: Increased acyl-CoA dehydrogenase activity. No effect on substrate specificity.
- 174:183 (vs. 121:130, 10% identical) binding in other chain
- S183 (≠ Y130) binding
- WIS 207:209 (≠ VVQ 154:156) binding in other chain
- S210 (≠ H157) mutation to N: Increased acyl-CoA dehydrogenase activity. Changed substrate specificity.
- Y229 (≠ D177) binding
- L255 (≠ G203) to F: in SBCADD; loss of protein expression; loss of 2-methylbutyryl-CoA dehydrogenase activity; dbSNP:rs137852649
- Y283 (≠ F228) binding
- NEGR 291:294 (≠ --GV 238:239) binding
- I316 (≠ L261) to V: in dbSNP:rs1131430
- R319 (= R264) binding
- Q330 (= Q275) binding
- EWMGG 387:391 (≠ QLHGG 333:337) binding
Sites not aligning to the query:
- 1:33 modified: transit peptide, Mitochondrion
- 13 R → K: in dbSNP:rs12263012
- 416 A→T: Increased acyl-CoA dehydrogenase activity. No effect on substrate specificity.
- 416:418 binding in other chain
2jifA Structure of human short-branched chain acyl-coa dehydrogenase (acadsb)
29% identity, 92% coverage: 6:349/375 of query aligns to 7:352/381 of 2jifA
- active site: L125 (≠ H123), S126 (≠ G124), G242 (= G238)
- binding coenzyme a persulfide: S132 (≠ Y130), S134 (≠ L132), Y178 (≠ D177), Y232 (≠ F228), I236 (= I235), L239 (vs. gap), N240 (vs. gap), R243 (≠ V239)
- binding flavin-adenine dinucleotide: F123 (≠ L121), L125 (≠ H123), S126 (≠ G124), G131 (≠ H129), S132 (≠ Y130), W156 (≠ V154), I157 (≠ V155), S158 (≠ Q156), K201 (≠ I200), T209 (vs. gap), R268 (= R264), F271 (= F267), L275 (≠ I271), F278 (= F274), L281 (= L277), E336 (≠ Q333), W337 (≠ L334), G340 (= G337)
Sites not aligning to the query:
P28330 Long-chain specific acyl-CoA dehydrogenase, mitochondrial; LCAD; EC 1.3.8.8 from Homo sapiens (Human) (see 2 papers)
30% identity, 82% coverage: 40:347/375 of query aligns to 86:399/430 of P28330
- E291 (≠ G238) active site, Proton acceptor; mutation to Q: Loss of long-chain-acyl-CoA dehydrogenase activity. No effect on protein abundance. No effect on solubility. No effect on substrate binding.
- S303 (≠ M250) to T: in dbSNP:rs1801204
- K333 (≠ R280) to Q: in dbSNP:rs2286963
8sgrA Isovaleryl-CoA dehydrogenase, mitochondrial (see paper)
28% identity, 92% coverage: 6:351/375 of query aligns to 13:365/393 of 8sgrA
- binding flavin-adenine dinucleotide: S135 (≠ G124), G140 (≠ H129), S141 (≠ Y130), W165 (≠ V154), T167 (≠ Q156), R279 (= R264), F282 (= F267), I286 (= I271), F289 (= F274), Q347 (= Q333), C348 (≠ L334), G351 (= G337)
Sites not aligning to the query:
1ivhA Structure of human isovaleryl-coa dehydrogenase at 2.6 angstroms resolution: structural basis for substrate specificity (see paper)
28% identity, 92% coverage: 6:351/375 of query aligns to 9:361/387 of 1ivhA
- active site: M130 (≠ H123), S131 (≠ G124), E249 (≠ G238)
- binding coenzyme a persulfide: S137 (≠ Y130), S185 (≠ D177), R186 (≠ A178), V239 (≠ F228), Y240 (≠ A229), M243 (≠ E232), E249 (≠ G238), R250 (≠ V239)
- binding flavin-adenine dinucleotide: L128 (= L121), M130 (≠ H123), S131 (≠ G124), G136 (≠ H129), S137 (≠ Y130), W161 (≠ V154), T163 (≠ Q156), R275 (= R264), F278 (= F267), F285 (= F274), M288 (≠ L277), Q343 (= Q333), C344 (≠ L334), G347 (= G337)
Sites not aligning to the query:
P26440 Isovaleryl-CoA dehydrogenase, mitochondrial; IVD; Butyryl-CoA dehydrogenase; EC 1.3.8.4; EC 1.3.8.1 from Homo sapiens (Human) (see 5 papers)
28% identity, 92% coverage: 6:351/375 of query aligns to 46:398/426 of P26440
- 165:174 (vs. 121:130, 40% identical) binding
- S174 (≠ Y130) binding
- WIT 198:200 (≠ VVQ 154:156) binding
- SR 222:223 (≠ DA 177:178) binding
- G250 (= G202) to A: in IVA; uncertain significance
- Y277 (≠ A229) binding
- DLER 284:287 (≠ GCGV 236:239) binding
- E286 (≠ G238) active site, Proton acceptor; mutation to D: Residual isovaleryl-CoA dehydrogenase activity.; mutation to G: Loss of isovaleryl-CoA dehydrogenase activity. Does not affect isovaleryl-CoA dehydrogenase activity; when associated with 407-E.; mutation to Q: Loss of isovaleryl-CoA dehydrogenase activity.
- A291 (≠ C243) to V: in IVA; uncertain significance; dbSNP:rs886042098
- R312 (= R264) binding
- Q323 (= Q275) binding
- I379 (= I332) to T: in IVA; uncertain significance
- QCFGG 380:384 (≠ QLHGG 333:337) binding
- R398 (≠ K351) to Q: in IVA; uncertain significance; dbSNP:rs1477527791
Sites not aligning to the query:
- 1:32 modified: transit peptide, Mitochondrion
- 403 Y → N: in IVA; uncertain significance
- 407 A→E: Does not affect isovaleryl-CoA dehydrogenase activity; when associated with 286-D.
- 407:408 binding
- 409:411 binding
6wy8C Tcur3481-tcur3483 steroid acad (see paper)
33% identity, 99% coverage: 1:370/375 of query aligns to 1:354/364 of 6wy8C
Sites not aligning to the query:
6wy9B Tcur3481-tcur3483 steroid acad g363a variant (see paper)
33% identity, 95% coverage: 16:370/375 of query aligns to 12:351/361 of 6wy9B
Sites not aligning to the query:
4ktoA Crystal structure of a putative isovaleryl-coa dehydrogenase (psi- nysgrc-012251) from sinorhizobium meliloti 1021
31% identity, 92% coverage: 1:345/375 of query aligns to 6:345/377 of 4ktoA
- active site: M130 (≠ H123), S131 (≠ G124), E239 (≠ G238)
- binding flavin-adenine dinucleotide: L128 (= L121), M130 (≠ H123), S131 (≠ G124), M155 (≠ A153), W156 (≠ V154), T158 (≠ Q156), R265 (= R264), F268 (= F267), I272 (= I271), F275 (= F274), M278 (≠ L277), Q333 (= Q333), A334 (≠ L334), G337 (= G337)
Sites not aligning to the query:
7y0bA Crystal structure of human short-chain acyl-coa dehydrogenase
27% identity, 81% coverage: 45:349/375 of query aligns to 42:355/385 of 7y0bA
- binding (2S,3R,4R,5S,6R)-2-[4-chloranyl-3-[[4-[(3S)-oxolan-3-yl]oxyphenyl]methyl]phenyl]-6-(hydroxymethyl)oxane-3,4,5-triol: M343 (≠ I338), T347 (≠ W342), E348 (= E343)
- binding flavin-adenine dinucleotide: F125 (≠ L121), L127 (≠ H123), S128 (≠ G124), G133 (≠ H129), S134 (≠ Y130), W158 (≠ V154), T160 (≠ Q156), R270 (= R264), F273 (= F267), L280 (≠ F274), V282 (≠ A276), Q338 (= Q333), I339 (≠ L334), G342 (= G337)
Sites not aligning to the query:
P16219 Short-chain specific acyl-CoA dehydrogenase, mitochondrial; SCAD; Butyryl-CoA dehydrogenase; EC 1.3.8.1 from Homo sapiens (Human) (see 3 papers)
27% identity, 81% coverage: 45:349/375 of query aligns to 69:382/412 of P16219
- G90 (= G66) to S: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs121908005
- E104 (≠ S77) natural variant: Missing (in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs387906308)
- 152:161 (vs. 121:130, 30% identical) binding in other chain
- R171 (≠ E140) to W: 69% of wild-type acyl-CoA dehydrogenase activity; confers susceptibility to ethylmalonicaciduria; dbSNP:rs1800556
- WIT 185:187 (≠ VVQ 154:156) binding in other chain
- A192 (= A161) to V: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs28940874
- G209 (= G179) to S: 86% of wild-type acyl-CoA dehydrogenase activity; confers susceptibility to ethylmalonicaciduria; dbSNP:rs1799958
- R297 (= R264) binding
- Q308 (= Q275) binding in other chain
- R325 (= R292) to W: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs121908006
- S353 (≠ G321) to L: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs28941773
- QILGG 365:369 (≠ QLHGG 333:337) binding
- R380 (vs. gap) to W: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs28940875
Sites not aligning to the query:
- 1:24 modified: transit peptide, Mitochondrion
- 394:396 binding in other chain
7y0aC Crystal structure of human short-chain acyl-coa dehydrogenase
27% identity, 81% coverage: 45:349/375 of query aligns to 45:358/387 of 7y0aC
- binding flavin-adenine dinucleotide: F128 (≠ L121), L130 (≠ H123), S131 (≠ G124), G136 (≠ H129), S137 (≠ Y130), W161 (≠ V154), T163 (≠ Q156), T214 (≠ A205), R273 (= R264), F276 (= F267), L280 (≠ I271), L283 (≠ F274), V285 (≠ A276), Q341 (= Q333), I342 (≠ L334), G345 (= G337)
Sites not aligning to the query:
8sgsA Short-chain specific acyl-CoA dehydrogenase, mitochondrial (see paper)
27% identity, 81% coverage: 45:349/375 of query aligns to 39:352/381 of 8sgsA
- binding coenzyme a: S131 (≠ Y130), A133 (≠ L132), N177 (≠ D177), F231 (= F228), M235 (≠ E232), L238 (≠ I235), I312 (≠ E310)
- binding flavin-adenine dinucleotide: F122 (≠ L121), L124 (≠ H123), S125 (≠ G124), G130 (≠ H129), S131 (≠ Y130), W155 (≠ V154), T157 (≠ Q156), R267 (= R264), F270 (= F267), L274 (≠ I271), L277 (≠ F274), Q335 (= Q333), I336 (≠ L334), G338 (= G336), G339 (= G337)
Sites not aligning to the query:
2vigB Crystal structure of human short-chain acyl coa dehydrogenase
27% identity, 81% coverage: 45:349/375 of query aligns to 36:342/371 of 2vigB
- active site: L121 (≠ H123), S122 (≠ G124), G231 (= G238)
- binding coenzyme a persulfide: S128 (≠ Y130), F221 (= F228), M225 (≠ E232), Q226 (≠ Y233), L228 (≠ I235), D229 (≠ G236), R232 (≠ V239)
- binding flavin-adenine dinucleotide: L121 (≠ H123), S122 (≠ G124), G127 (≠ H129), S128 (≠ Y130), W152 (≠ V154), T154 (≠ Q156), R257 (= R264), F260 (= F267), L264 (≠ I271), L267 (≠ F274), Q325 (= Q333), I326 (≠ L334), G329 (= G337)
Sites not aligning to the query:
4n5fA Crystal structure of a putative acyl-coa dehydrogenase with bound fadh2 from burkholderia cenocepacia j2315
30% identity, 90% coverage: 1:339/375 of query aligns to 2:343/378 of 4n5fA
Sites not aligning to the query:
P15651 Short-chain specific acyl-CoA dehydrogenase, mitochondrial; SCAD; Butyryl-CoA dehydrogenase; EC 1.3.8.1 from Rattus norvegicus (Rat) (see 2 papers)
26% identity, 81% coverage: 45:349/375 of query aligns to 69:381/412 of P15651
Sites not aligning to the query:
- 1:24 modified: transit peptide, Mitochondrion
- 392 active site, Proton acceptor
- 394:396 binding
4l1fA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
27% identity, 94% coverage: 1:352/375 of query aligns to 1:355/380 of 4l1fA
- active site: L125 (≠ H123), T126 (≠ G124), G242 (= G238)
- binding coenzyme a persulfide: T132 (≠ Y130), H179 (vs. gap), F232 (= F228), M236 (≠ E232), E237 (≠ Y233), L239 (≠ I235), D240 (≠ G236), R243 (≠ V239)
- binding flavin-adenine dinucleotide: F123 (≠ L121), L125 (≠ H123), T126 (≠ G124), G131 (≠ H129), T132 (≠ Y130), F156 (≠ V154), I157 (≠ V155), T158 (≠ Q156), R268 (= R264), Q270 (= Q266), F271 (= F267), I275 (= I271), F278 (= F274), L281 (= L277), Q336 (= Q333), I337 (≠ L334), G340 (= G337)
- binding 1,3-propandiol: L5 (≠ H5), Q10 (≠ R10)
Sites not aligning to the query:
1jqiA Crystal structure of rat short chain acyl-coa dehydrogenase complexed with acetoacetyl-coa (see paper)
26% identity, 81% coverage: 45:349/375 of query aligns to 42:354/384 of 1jqiA
- binding acetoacetyl-coenzyme a: L95 (= L91), F125 (≠ L121), S134 (≠ Y130), F234 (= F228), M238 (≠ E232), Q239 (≠ Y233), L241 (≠ I235), D242 (≠ G236), R245 (≠ V239)
- binding flavin-adenine dinucleotide: F125 (≠ L121), L127 (≠ H123), S128 (≠ G124), G133 (≠ H129), S134 (≠ Y130), W158 (≠ V154), T160 (≠ Q156), R270 (= R264), F273 (= F267), L280 (≠ F274), Q338 (= Q333), I339 (≠ L334), G342 (= G337)
Sites not aligning to the query:
3pfdC Crystal structure of an acyl-coa dehydrogenase from mycobacterium thermoresistibile bound to reduced flavin adenine dinucleotide solved by combined iodide ion sad mr (see paper)
29% identity, 77% coverage: 59:346/375 of query aligns to 49:339/369 of 3pfdC
- active site: L116 (≠ H123), S117 (≠ G124), T233 (≠ G238)
- binding dihydroflavine-adenine dinucleotide: Y114 (≠ L121), L116 (≠ H123), S117 (≠ G124), G122 (≠ H129), S123 (≠ Y130), W147 (≠ V154), I148 (≠ V155), T149 (≠ Q156), R259 (= R264), F262 (= F267), V266 (≠ I271), N269 (≠ F274), Q326 (= Q333), L327 (= L334), G330 (= G337)
Sites not aligning to the query:
Query Sequence
>BPHYT_RS13555 BPHYT_RS13555 acyl-CoA dehydrogenase
MNFQHTEDRRMLADTLNRFVSEQYAFSTRDRIAQSDQGYSGELWSRFAELGIIGALFDEA
DGGFGGGGFDIAVVFESLGRGLVVEPFLDTLIVGRAIARSGNEAQKAVLGELIDGSRIVA
LAHGEPDSHYELSRVSARAERSGSTWQLNGAKAVVQHGEHASLFLVSARTGGDDDSDAGI
TLFLVPRDAAGVSVRGYRKIDGGRAAEVTLDNVTLSDDALLGTPDAGFATLEYAIGCGVF
ALCSEAVGAMDVAKDYTLEYLRTRKQFGVPIGSFQALQHRMADLLLEIEQARSAVINAAA
ALGAERKVRERAVSAAKYSIGRIGALVAEECIQLHGGIGMTWELPLAHYAKRLVMIDHQL
GDEDHHLERFVALGR
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory