SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS13705 BPHYT_RS13705 chorismate synthase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 8 hits to proteins with known functional sites (download)

1um0A Crystal structure of chorismate synthase complexed with fmn (see paper)
47% identity, 93% coverage: 4:345/366 of query aligns to 2:348/365 of 1um0A

query
sites
1um0A

N

N

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T

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L

G

G

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L

F

F

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G

G

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H

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-

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x
G

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K

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binding flavin mononucleotide: H104 (= H106), R123 (= R125), S125 (= S127), N241 (= N238), G242 (≠ A239), L280 (= L277), G281 (= G278), K296 (= K293), T298 (= T295), P299 (≠ S296), S300 (= S297), I327 (≠ V324), R330 (= R327)

P56122 Chorismate synthase; CS; 5-enolpyruvylshikimate-3-phosphate phospholyase; EC 4.2.3.5 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see paper)
47% identity, 93% coverage: 4:345/366 of query aligns to 2:348/365 of P56122

query
sites
P56122

N

N

T

T

L

L

G

G

T

R

L

F

F

L

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L

T

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D

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A

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Q

E

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E

E

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K

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G

G

G

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E

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E

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G

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F

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F

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S

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K

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Y

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S

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K

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-

G

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A

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L

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A

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L

RSS 123:125 (= RSS 125:127) binding
KPTPS 296:300 (≠ KPTSS 293:297) binding

Q12640 Chorismate synthase aro-2; 5-enolpyruvylshikimate-3-phosphate phospholyase; EC 1.5.1.38; EC 4.2.3.5 from Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) (see 3 papers)
39% identity, 95% coverage: 4:350/366 of query aligns to 2:396/432 of Q12640

query
sites
Q12640

N

S

T

T

L

F

G

G

T

H

L

Y

F

F

T

R

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V

T

T

T

T

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Y

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G

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E

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S

H
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H

G

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P

K

A

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I

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L

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P

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M

N

N

T

E

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D

Q

Q

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R

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K

K

D

D

Y

Y

G

G

N

N

-

K

I

T

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M

D

D

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I

F

Y

-

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R

R

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P

G

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H
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H

A

A

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D

Y

W

T

T

Y

Y

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E

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K

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Y

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G

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Y

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G

G

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G

G

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S
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S

A

A

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V

A

A

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A

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Y

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E

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N

N

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P

F

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F

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-

L

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-

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N

D

A

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Y

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D

D

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G

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H

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N

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D

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P

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-

A

-

E

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N

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E

E

L

I

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P

P

E

S

G

V

F

A

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A

G

S

-

G

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A

-

A

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N

-

G

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I

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P

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P

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K

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L

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T

-

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K

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T

N

N

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G

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G

G

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G

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D

P

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I

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A

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A

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A

A

Q

R

S16 (= S18) mutation to A: Does not affect the flavin reductase activity but reduces the chorismate synthase activity 70-fold.
H17 (= H19) active site; mutation to A: Reduces the chorismate synthase activity 20-fold.
H106 (= H106) active site; mutation to A: Reduces the chorismate synthase activity 10-fold.
S127 (= S127) mutation to A: Does not affect the flavin reductase activity but reduces the chorismate synthase activity 6-fold.
D367 (= D321) active site; mutation D->A,N: Does not affect substrate binding but stringly reduces the chorismate synthase activity.

2qhfA Mycobacterium tuberculosis chorismate synthase in complex with nca
38% identity, 91% coverage: 14:345/366 of query aligns to 6:367/392 of 2qhfA

query
sites
2qhfA

T

T

F

A

G

G

E

E

S

S

H

H

G

G

P

R

A

A

I

L

G

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C

A

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A

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D

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L

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R

H

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M

R

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Q

F

E

E

E

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D

D

K

A

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L

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S

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T

L

G

G

A

G

P

P

I

I

A

A

L

I

L

E

I

I

R

G

N

N

T

T

D

E

-

W

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P

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K

-

W

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E

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T

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V

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M

-

A

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A

R

D

S

P

K

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D

D

Y

P

G

A

N

E

I

L

A

A

D

D

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F

A

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R

-

N

-

A

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P

-

L

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T

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P

R

R

P

P

G

G

H

H

A

A

D

D

Y

Y

T

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Y

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K

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Y

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D

D

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A

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P

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L

G

E

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A
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A

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E

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T

A

A

P

A

T

R

V

V

A

A

A

A

G

G

A

T

V

V

A

A

K

R

K

A

W

F

L

L

R

R

E

Q

K

A

F

L

G

G

T

V

E

E

I

V

H

L

G

S

Y

H

M

V

A

I

A

S

L

I

G

G

E

A

I

S

-

A

-

P

-

Y

-

E

-

G

-

P

-

P

-

P

-

R

-

A

-

E

D

D

V

L

P

P

F

A

V

I

D

D

W

A

A

S

H

P

V

V

R

R

E

A

N

Y

P

D

F

-

F

K

A

A

P

A

N

E

A

A

Q

D

I

M

V

I

P

A

Q

Q

L

I

E

E

T

-

Y

-

M

-

D

-

A

A

L

A

R

K

K

K

D

D

G

G

D

D

S

T

I

L

G

G

A

G

R

V

I

V

N

E

V

A

V

V

A

A

S

L

G

G

V

L

P

P

V

V

G

G

L

L

G

G

E

S

P

F

L

T

F

S

-

G

-

D

D

H

R

R

L

L

D

D

A

S

D

Q

I

L

A

A

H

A

A

A

M

V

M

M

G

G

I
|
I

N
x
Q

A

A

V

I

K

K

G

G

V

V

E

E

I

I

G

G

A

D

G

G

F

F

A

Q

S

T

I

A

A

R

Q

R

R

R

G

G

S

S

V

R

H

A

G

H

D

D

E

E

L

M

T

Y

-

P

-

G

P

P

E

D

G

G

F

V

V

V

-

R

-

S

G

T

N

N

H

R

A

A

G

G

G

G

V

L

L

E

G

G

G

G

I

M

S

T

T

N

G

G

Q

Q

D

P

V

L

T

R

V

V

S

R

I

A

A

A

I
x
M

K

K

P

P

T
x
I

S

S

S

T

I

V

R

P

T

R

P

A

R

L

R

A

S

T

I

V

D

D

K

L

A

A

G

T

Q

G

P

D

V

E

V

A

V

V

E

A

T

I

F

H

G

Q

R
|
R

H

S

D

D

P

V

C

C

V

A

G

V

I

P

R

A

A

A

T

G

P

V

I

V

A

V

E

E

S

T

M

M

L

V

A

A

L

L

V

V

L

L

I

A

D

R

H

A

A

A

L

L

binding nicotinamide: R46 (= R54), A138 (= A128), I255 (= I237), Q256 (≠ N238), M314 (≠ I292), I317 (≠ T295), R341 (= R319)

P9WPY1 Chorismate synthase; CS; 5-enolpyruvylshikimate-3-phosphate phospholyase; EC 4.2.3.5 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
38% identity, 91% coverage: 14:345/366 of query aligns to 6:367/401 of P9WPY1

query
sites
P9WPY1

T

T

F

A

G

G

E

E

S

S

H

H

G

G

P

R

A

A

I

L

G

V

C

A

V

V

I

V

D

E

G

G

C

M

P

V

P

A

G

G

M

V

A

H

L

V

N

T

E

S

A

A

D

D

I

I

Q

A

F

D

E

Q

L

L

D

A

R

R

R
|
R

K

R

P

L

G

G

T

Y

S

G

R
|
R

H

G

V

A

T

R

Q

M

R

T

Q

F

E

E

E

R

D

D

K

A

V

V

E

T

I

V

L

L

S

S

G

G

V

I

F

R

E

H

G

G

Q

S

T

T

T

L

G

G

A

G

P

P

I

I

A

A

L

I

L

E

I

I

R

G

N

N

T

T

D

E

-

W

-

P

-

K

-

W

-

E

-

T

-

V

-

M

-

A

Q

A

R

D

S

P

K

V

D

D

Y

P

G

A

N

E

I

L

A

A

D

D

T

V

F

A

-

R

-

N

-

A

-

P

-

L

-

T

-

R

-

P

R

R

P

P

G

G

H

H

A

A

D

D

Y

Y

T

A

Y

G

W

M

Q

L

K

K

Y

Y

G

G

I

F

R

D

D

D

Y

A

R

R

G

P

G

V

-

L

G

E

R

R

S

A

S

S

A

A

R

R

L

E

T

T

A

A

P

A

T

R

V

V

A

A

A

A

G

G

A

T

V

V

A

A

K

R

K

A

W

F

L

L

R

R

E

Q

K

A

F

L

G

G

T

V

E

E

I

V

H

L

G

S

Y

H

M

V

A

I

A

S

L

I

G

G

E

A

I

S

-

A

-

P

-

Y

-

E

-

G

-

P

-

P

-

P

-

R

-

A

-

E

D

D

V

L

P

P

F

A

V

I

D

D

W

A

A

S

H

P

V

V

R

R

E

A

N

Y

P

D

F

-

F

K

A

A

P

A

N

E

A

A

Q

D

I

M

V

I

P

A

Q

Q

L

I

E

E

T

-

Y

-

M

-

D

-

A

A

L

A

R

K

K

K

D

D

G

G

D

D

S

T

I

L

G

G

A

G

R

V

I

V

N

E

V

A

V

V

A

A

S

L

G

G

V

L

P

P

V

V

G

G

L

L

G

G

E

S

P

F

L

T

F

S

-

G

-

D

D

H

R

R

L

L

D

D

A

S

D

Q

I

L

A

A

H

A

A

A

M

V

M

M

G

G

I

I

N
x
Q

A
|
A

V

I

K

K

G

G

V

V

E

E

I

I

G

G

A

D

G

G

F

F

A

Q

S

T

I

A

A

R

Q

R

R

R

G

G

S

S

V

R

H

A

G

H

D

D

E

E

L

M

T

Y

-

P

-

G

P

P

E

D

G

G

F

V

V

V

-

R

-

S

G

T

N

N

H

R

A

A

G

G

G

G

V

L

L

E

G

G

G

G

I

M

S

T

T

N

G

G

Q

Q

D

P

V

L

T

R

V

V

S

R

I

A

A

A

I

M

K
|
K

P
|
P

T
x
I

S
|
S

S
x
T

I

V

R

P

T

R

P

A

R

L

R

A

S

T

I

V

D

D

K

L

A

A

G

T

Q

G

P

D

V

E

V

A

V

V

E

A

T

I

F

H

G

Q

R
|
R

H

S

D

D

P

V

C

C

V

A

G

V

I

P

R

A

A

A

T

G

P

V

I

V

A

V

E

E

S

T

M

M

L

V

A

A

L

L

V

V

L

L

I

A

D

R

H

A

A

A

L

L

R40 (= R48) binding
R46 (= R54) binding
QA 256:257 (≠ NA 238:239) binding
KPIST 315:319 (≠ KPTSS 293:297) binding
R341 (= R319) binding

2o12A Mycobacterium tuberculosis chorismate synthase in complex with fmn
38% identity, 91% coverage: 14:345/366 of query aligns to 6:361/386 of 2o12A

query
sites
2o12A

T

T

F

A

G

G

E

E

S

S

H

H

G

G

P

R

A

A

I

L

G

V

C

A

V

V

I

V

D

E

G

G

C

M

P

V

P

A

G

G

M

V

A

H

L

V

N

T

E

S

A

A

D

D

I

I

Q

A

F

D

E

Q

L

L

D

A

R

R

R

R

K

R

P

L

G

G

T

Y

S

G

R

R

H

-

V

-

T

-

Q

-

R

-

Q

-

E

E

E

R

D

D

K

A

V

V

E

T

I

V

L

L

S

S

G

G

V

I

F

R

E

H

G

G

Q

S

T

T

T

L

G

G

A

G

P

P

I

I

A

A

L

I

L

E

I

I

R

G

N

N

T

T

D

E

-

W

-

P

-

K

-

W

-

E

-

T

-

V

-

M

-

A

Q

A

R

D

S

P

K

V

D

D

Y

P

G

A

N

E

I

L

A

A

D

D

T

V

F

A

R

R

-

N

-

A

-

P

-

L

-

T

-

R

-

P

-

R

P

P

G

G

H
|
H

A

A

D

D

Y

Y

T

A

Y

G

W

M

Q

L

K

K

Y

Y

G

G

I

F

R

D

D

D

Y

A

R

R

G

P

G

V

-

L

G

E

R

R

S

A

S

S

A
|
A

R

R

L

E

T

T

A

A

P

A

T

R

V

V

A

A

A

A

G

G

A

T

V

V

A

A

K

R

K

A

W

F

L

L

R

R

E

Q

K

A

F

L

G

G

T

V

E

E

I

V

H

L

G

S

Y

H

M

V

A

I

A

S

L

I

G

G

E

A

I

S

-

A

-

P

-

Y

-

E

-

G

-

P

-

P

-

P

-

R

-

A

-

E

D

D

V

L

P

P

F

A

V

I

D

D

W

A

A

S

H

P

V

V

R

R

E

A

N

Y

P

D

F

K

F

-

A

A

P

A

N

E

A

A

Q

D

I

M

V

I

P

A

Q

Q

L

I

E

E

T

-

Y

-

M

-

D

-

A

A

L

A

R

K

K

K

D

D

G

G

D

D

S

T

I

L

G

G

A

G

R

V

I

V

N

E

V

A

V

V

A

A

S

L

G

G

V

L

P

P

V

V

G

G

L

L

G

G

E

S

P

F

L

T

F

S

-

G

-

D

D

H

R

R

L

L

D

D

A

S

D

Q

I

L

A

A

H

A

A

A

M

V

M

M

G

G

I

I

N
x
Q

A
|
A

V

I

K

K

G

G

V

V

E

E

I

I

G

G

A

D

G

G

F

F

A

Q

S

T

I

A

A

R

Q

R

R

R

G

G

S

S

V

R

H

A

G

H

D

D

E

E

L

M

T

Y

-

P

-

G

P

P

E

D

G

G

F

V

V

V

-

R

-

S

G

T

N

N

H

R

A

A

G

G

G

G

V

L

L

E

G

G

G

G

I

M

S

T

T

N

G

G

Q

Q

D

P

V

L

T

R

V

V

S

R

I

A

A

A

I
x
M

K
|
K

P

P

T
x
I

S

S

S
x
T

I

V

R

P

T

R

P

A

R

L

R

A

S

T

I

V

D

D

K

L

A

A

G

T

Q

G

P

D

V

E

V

A

V

V

E

A

T

I

F

H

G

Q

R
|
R

H

S

D

D

P

V

C

C

V

A

G

V

I

P

R

A

A

A

T

G

P

V

I

V

A

V

E

E

S

T

M

M

L

V

A

A

L

L

V

V

L

L

I

A

D

R

H

A

A

A

L

L

binding flavin mononucleotide: H109 (= H106), A132 (= A128), Q250 (≠ N238), A251 (= A239), M308 (≠ I292), K309 (= K293), I311 (≠ T295), T313 (≠ S297), R335 (= R319)

1qxoA Crystal structure of chorismate synthase complexed with oxidized fmn and epsp (see paper)
36% identity, 91% coverage: 14:345/366 of query aligns to 5:363/388 of 1qxoA

query
sites
1qxoA

T

T

F

A

G

G

E

E

S

S

H

H

G

G

P

P

A

R

I

L

G

T

C

A

V

I

I

I

D

E

G

G

C

I

P

P

P

A

G

G

M

L

A

P

L

L

N

T

E

A

A

E

D

D

I

I

Q

N

F

E

E

D

L

L

D

R

R

R

R
|
R

K

Q

P

G

G

G

T

Y

S

G

R
|
R

H

G

V

G

T
x
R

Q
x
M

R

K

Q

I

E

E

E

N

D

D

K

Q

V

V

E

V

I

F

L

T

S

S

G

G

V

V

F

R

E

H

G

G

Q

K

T

T

T

T

G

G

A

A

P

P

I

I

A

T

L

M

L

D

I

V

R

I

N

N

T

K

D

D

Q

H

R

Q

S

K

-

W

-

L

-

D

-

I

K

M

D

S

Y

A

G

E

N

D

I

I

A

E

D

D

T

R

F

L

-

K

-

S

-

K

-

R

-

K

-

I

-

T

-

H

-

P

R

R

P

P

G

G

H
|
H

A

A

D

D

Y

L

T

V

Y

G

W

G

Q

I

K

K

Y

Y

G

R

I

F

R

D

D

D

Y

L

R

R

G

N

G

S

-

L

G

E

R

R

S

S

S

S

A
|
A

R
|
R

L

E

T

T

A

T

P

M

T

R

V

V

A

A

A

V

G

G

A

A

V

V

A

A

K

K

K

R

W

L

L

L

R

A

E

E

K

-

F

L

G

D

T

M

E

E

I

I

H

A

G

N

Y

H

M

V

A

V

A

V

L

F

G

G

-

G

-

K

E

E

I

I

D

D

V

V

P

P

-

E

F

N

V

L

D

T

W

V

A

A

H

E

V

I

R

K

E

Q

N

R

P

A

F

A

F

Q

A

S

P

E

-

V

-

S

-

I

-

V

N

N

A

Q

Q

E

I

R

V

E

P

Q

Q

E

L

I

E

K

T

D

Y

Y

M

I

D

D

A

Q

L

I

R

K

K

R

D

D

G

G

D

D

S

T

I

I

G

G

A

G

R

V

I

V

N

E

V

T

V

V

A

V

S

G

G

G

V

V

P

P

V

V

G

G

L

L

G

G

E

S

P

Y

L

V

-

Q

F

W

D

D

R

R

-

K

L

L

D

D

A

A

D

R

I

L

A

A

H

Q

A

A

M

V

M

V

G

S

I

I

N
|
N

A
|
A

V

F

K

K

G

G

V

V

E

E

I

F

G

G

A

L

G

G

F

F

A

E

S

A

I

G

A

Y

Q

R

R

K

G

G

S

S

V

Q

H

V

G

M

D

D

E

E

L

I

-

L

-

W

-

S

T

K

P

E

E

D

G

G

F

Y

V

T

-

R

-

R

G

T

N

N

H

N

A

L

G

G

G

G

V

F

L

E

G

G

G

G

I

M

S

T

T

N

G

G

Q

Q

D

P

V

I

T

V

V

V

S

R

I

G

A

V

I
x
M

K
|
K

P

P

T
x
I

S
x
P

S
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binding 5-[(1-carboxyvinyl)oxy]-4-hydroxy-3-(phosphonooxy)cyclohex-1-ene-1-carboxylic acid: R39 (= R48), R45 (= R54), R48 (≠ T57), M49 (≠ Q58), A133 (= A128), R134 (= R129)
binding flavin mononucleotide: H110 (= H106), A133 (= A128), N251 (= N238), A252 (= A239), M310 (≠ I292), K311 (= K293), I313 (≠ T295), P314 (≠ S296), T315 (≠ S297)

P0A2Y6 Chorismate synthase; CS; 5-enolpyruvylshikimate-3-phosphate phospholyase; EC 4.2.3.5 from Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) (see paper)
36% identity, 91% coverage: 14:345/366 of query aligns to 5:363/388 of P0A2Y6

query
sites
P0A2Y6

T

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NA 251:252 (= NA 238:239) binding
G296 (= G278) binding
KPIPT 311:315 (≠ KPTSS 293:297) binding

Query Sequence

>BPHYT_RS13705 BPHYT_RS13705 chorismate synthase
MSGNTLGTLFTVTTFGESHGPAIGCVIDGCPPGMALNEADIQFELDRRKPGTSRHVTQRQ
EEDKVEILSGVFEGQTTGAPIALLIRNTDQRSKDYGNIADTFRPGHADYTYWQKYGIRDY
RGGGRSSARLTAPTVAAGAVAKKWLREKFGTEIHGYMAALGEIDVPFVDWAHVRENPFFA
PNAQIVPQLETYMDALRKDGDSIGARINVVASGVPVGLGEPLFDRLDADIAHAMMGINAV
KGVEIGAGFASIAQRGSVHGDELTPEGFVGNHAGGVLGGISTGQDVTVSIAIKPTSSIRT
PRRSIDKAGQPVVVETFGRHDPCVGIRATPIAESMLALVLIDHALRHRAQCGDVAVSTPK
IAASAP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory