SitesBLAST
Comparing BPHYT_RS13915 FitnessBrowser__BFirm:BPHYT_RS13915 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6demA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide bensulfuron methyl (see paper)
25% identity, 72% coverage: 59:534/657 of query aligns to 27:497/597 of 6demA
- active site: Y33 (≠ H65), G35 (vs. gap), G36 (= G66), A37 (≠ N67), I38 (≠ V68), E59 (= E90), T82 (= T113), F121 (≠ L154), Q122 (= Q155), E123 (≠ Q156), K171 (≠ Q213), K228 (≠ D272), M264 (≠ V308), V291 (≠ T335), V407 (≠ A444), L432 (≠ Y469), G433 (≠ S470), M435 (= M472), D460 (= D497), N487 (= N524), E489 (≠ G526), Q490 (≠ Y527), M492 (≠ C529), V493 (≠ I530), W496 (≠ L533)
- binding methyl 2-[(4,6-dimethoxypyrimidin-2-yl)carbamoylsulfamoylmethyl]benzoate: M264 (≠ V308), D289 (= D333), R290 (≠ F334), M492 (≠ C529), W496 (≠ L533)
- binding flavin-adenine dinucleotide: R161 (≠ L203), G217 (= G262), A218 (≠ G263), G219 (= G264), N222 (≠ Y267), T244 (= T288), L245 (≠ Q289), Q246 (≠ A290), L262 (= L306), G284 (= G328), A285 (≠ T329), R286 (= R330), D288 (≠ Q332), R290 (≠ F334), V291 (≠ T335), E317 (vs. gap), I318 (≠ V350), N322 (≠ A354), D336 (= D368), V337 (≠ A369), M412 (≠ L454), G430 (≠ Y467)
- binding magnesium ion: D460 (= D497), N487 (= N524), E489 (≠ G526)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V407 (≠ A444), G408 (= G445), Q409 (≠ T446), H410 (= H452), M435 (= M472), G459 (= G496), D460 (= D497), A461 (≠ G498), S462 (= S499), M465 (= M502), N487 (= N524), E489 (≠ G526), Q490 (≠ Y527), G491 (= G528), M492 (≠ C529), V493 (≠ I530)
Sites not aligning to the query:
6delA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide chlorimuron ethyl (see paper)
25% identity, 72% coverage: 59:534/657 of query aligns to 27:497/597 of 6delA
- active site: Y33 (≠ H65), G35 (vs. gap), G36 (= G66), A37 (≠ N67), I38 (≠ V68), E59 (= E90), T82 (= T113), F121 (≠ L154), Q122 (= Q155), E123 (≠ Q156), K171 (≠ Q213), K228 (≠ D272), M264 (≠ V308), V291 (≠ T335), V407 (≠ A444), L432 (≠ Y469), G433 (≠ S470), M435 (= M472), D460 (= D497), N487 (= N524), E489 (≠ G526), Q490 (≠ Y527), M492 (≠ C529), V493 (≠ I530), W496 (≠ L533)
- binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: D289 (= D333), R290 (≠ F334), W496 (≠ L533)
- binding flavin-adenine dinucleotide: R161 (≠ L203), G217 (= G262), A218 (≠ G263), G219 (= G264), N222 (≠ Y267), T244 (= T288), L245 (≠ Q289), Q246 (≠ A290), L262 (= L306), G284 (= G328), A285 (≠ T329), R286 (= R330), D288 (≠ Q332), R290 (≠ F334), V291 (≠ T335), E317 (vs. gap), I318 (≠ V350), N322 (≠ A354), D336 (= D368), V337 (≠ A369), M412 (≠ L454), G430 (≠ Y467)
- binding (3Z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl](formyl)amino}-3-sulfanylpent-3-en-1-yl trihydrogen diphosphate: V407 (≠ A444), G408 (= G445), Q409 (≠ T446), H410 (= H452), G433 (≠ S470), M435 (= M472), G459 (= G496), D460 (= D497), A461 (≠ G498), S462 (= S499), M465 (= M502), N487 (= N524), E489 (≠ G526), Q490 (≠ Y527), G491 (= G528), M492 (≠ C529), V493 (≠ I530)
- binding magnesium ion: D460 (= D497), N487 (= N524), E489 (≠ G526)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V407 (≠ A444), G408 (= G445), Q409 (≠ T446), H410 (= H452), G433 (≠ S470), M435 (= M472), G459 (= G496), D460 (= D497), A461 (≠ G498), S462 (= S499), M465 (= M502), N487 (= N524), E489 (≠ G526), Q490 (≠ Y527), G491 (= G528), M492 (≠ C529), V493 (≠ I530)
Sites not aligning to the query:
6denA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide iodomuron ethyl (see paper)
25% identity, 72% coverage: 59:534/657 of query aligns to 29:499/599 of 6denA
- active site: Y35 (≠ H65), G37 (vs. gap), G38 (= G66), A39 (≠ N67), I40 (≠ V68), E61 (= E90), T84 (= T113), F123 (≠ L154), Q124 (= Q155), E125 (≠ Q156), K173 (≠ Q213), K230 (≠ D272), M266 (≠ V308), V293 (≠ T335), V409 (≠ A444), L434 (≠ Y469), G435 (≠ S470), M437 (= M472), D462 (= D497), N489 (= N524), E491 (≠ G526), Q492 (≠ Y527), M494 (≠ C529), V495 (≠ I530), W498 (≠ L533)
- binding flavin-adenine dinucleotide: R163 (≠ L203), G219 (= G262), A220 (≠ G263), G221 (= G264), N224 (≠ Y267), T246 (= T288), L247 (≠ Q289), Q248 (≠ A290), L264 (= L306), G286 (= G328), A287 (≠ T329), R288 (= R330), D290 (≠ Q332), R292 (≠ F334), V293 (≠ T335), E319 (vs. gap), I320 (≠ V350), N324 (≠ A354), D338 (= D368), V339 (≠ A369), M414 (≠ L454), G432 (≠ Y467)
- binding ethyl 2-{[(4-iodo-6-methoxypyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: M266 (≠ V308), D291 (= D333), R292 (≠ F334), W498 (≠ L533)
- binding magnesium ion: D462 (= D497), N489 (= N524), E491 (≠ G526)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V409 (≠ A444), G410 (= G445), Q411 (≠ T446), H412 (= H452), G435 (≠ S470), M437 (= M472), G461 (= G496), D462 (= D497), A463 (≠ G498), S464 (= S499), N489 (= N524), E491 (≠ G526), Q492 (≠ Y527), G493 (= G528), M494 (≠ C529), V495 (≠ I530)
Sites not aligning to the query:
6derA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide metosulam (see paper)
25% identity, 72% coverage: 59:534/657 of query aligns to 29:500/600 of 6derA
- active site: Y35 (≠ H65), G37 (vs. gap), G38 (= G66), A39 (≠ N67), I40 (≠ V68), E61 (= E90), T84 (= T113), F123 (≠ L154), Q124 (= Q155), E125 (≠ Q156), K173 (≠ Q213), K231 (≠ D272), M267 (≠ V308), V294 (≠ T335), V410 (≠ A444), L435 (≠ Y469), G436 (≠ S470), M438 (= M472), D463 (= D497), N490 (= N524), E492 (≠ G526), Q493 (≠ Y527), M495 (≠ C529), V496 (≠ I530), W499 (≠ L533)
- binding flavin-adenine dinucleotide: R163 (≠ L203), G220 (= G262), A221 (≠ G263), G222 (= G264), N225 (≠ Y267), T247 (= T288), L248 (≠ Q289), Q249 (≠ A290), L265 (= L306), H268 (≠ T309), G287 (= G328), A288 (≠ T329), R289 (= R330), D291 (≠ Q332), R293 (≠ F334), V294 (≠ T335), E320 (vs. gap), I321 (≠ V350), N325 (≠ A354), G338 (≠ A367), D339 (= D368), V340 (≠ A369), Q414 (≠ K453), M415 (≠ L454), G433 (≠ Y467)
- binding Metosulam: R293 (≠ F334), M495 (≠ C529), W499 (≠ L533)
- binding magnesium ion: D463 (= D497), N490 (= N524), E492 (≠ G526)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V410 (≠ A444), G411 (= G445), Q412 (≠ T446), H413 (= H452), G436 (≠ S470), M438 (= M472), G462 (= G496), D463 (= D497), A464 (≠ G498), S465 (= S499), N490 (= N524), E492 (≠ G526), Q493 (≠ Y527), G494 (= G528), M495 (≠ C529), V496 (≠ I530)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V410 (≠ A444), G411 (= G445), Q412 (≠ T446), H413 (= H452), G436 (≠ S470), M438 (= M472), G462 (= G496), D463 (= D497), A464 (≠ G498), S465 (= S499), M468 (= M502), N490 (= N524), E492 (≠ G526), Q493 (≠ Y527), G494 (= G528), V496 (≠ I530)
Sites not aligning to the query:
6desA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide propoxycarbazone (see paper)
25% identity, 72% coverage: 59:534/657 of query aligns to 27:498/598 of 6desA
- active site: Y33 (≠ H65), G35 (vs. gap), G36 (= G66), A37 (≠ N67), I38 (≠ V68), E59 (= E90), T82 (= T113), F121 (≠ L154), Q122 (= Q155), E123 (≠ Q156), K171 (≠ Q213), K229 (≠ D272), M265 (≠ V308), V292 (≠ T335), V408 (≠ A444), L433 (≠ Y469), G434 (≠ S470), M436 (= M472), D461 (= D497), N488 (= N524), E490 (≠ G526), Q491 (≠ Y527), M493 (≠ C529), V494 (≠ I530), W497 (≠ L533)
- binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: M265 (≠ V308), D290 (= D333), R291 (≠ F334), W497 (≠ L533)
- binding flavin-adenine dinucleotide: R161 (≠ L203), G218 (= G262), A219 (≠ G263), G220 (= G264), N223 (≠ Y267), T245 (= T288), L246 (≠ Q289), Q247 (≠ A290), L263 (= L306), G285 (= G328), A286 (≠ T329), R287 (= R330), D289 (≠ Q332), R291 (≠ F334), V292 (≠ T335), E318 (vs. gap), I319 (≠ V350), N323 (≠ A354), D337 (= D368), V338 (≠ A369), Q412 (≠ K453), M413 (≠ L454), G431 (≠ Y467)
- binding magnesium ion: D461 (= D497), N488 (= N524), E490 (≠ G526)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V408 (≠ A444), G409 (= G445), Q410 (≠ T446), H411 (= H452), G434 (≠ S470), M436 (= M472), G460 (= G496), D461 (= D497), A462 (≠ G498), S463 (= S499), N488 (= N524), E490 (≠ G526), Q491 (≠ Y527), G492 (= G528), M493 (≠ C529), V494 (≠ I530)
Sites not aligning to the query:
6depA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide sulfometuron methyl (see paper)
25% identity, 72% coverage: 59:534/657 of query aligns to 27:498/598 of 6depA
- active site: Y33 (≠ H65), G35 (vs. gap), G36 (= G66), A37 (≠ N67), I38 (≠ V68), E59 (= E90), T82 (= T113), F121 (≠ L154), Q122 (= Q155), E123 (≠ Q156), K171 (≠ Q213), K229 (≠ D272), M265 (≠ V308), V292 (≠ T335), V408 (≠ A444), L433 (≠ Y469), G434 (≠ S470), M436 (= M472), D461 (= D497), N488 (= N524), E490 (≠ G526), Q491 (≠ Y527), M493 (≠ C529), V494 (≠ I530), W497 (≠ L533)
- binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D290 (= D333), R291 (≠ F334), M493 (≠ C529), W497 (≠ L533)
- binding flavin-adenine dinucleotide: R161 (≠ L203), G218 (= G262), A219 (≠ G263), G220 (= G264), N223 (≠ Y267), T245 (= T288), L246 (≠ Q289), Q247 (≠ A290), L263 (= L306), G264 (= G307), G285 (= G328), A286 (≠ T329), R287 (= R330), D289 (≠ Q332), R291 (≠ F334), V292 (≠ T335), E318 (vs. gap), I319 (≠ V350), N323 (≠ A354), D337 (= D368), V338 (≠ A369), M413 (≠ L454), G431 (≠ Y467)
- binding magnesium ion: D461 (= D497), N488 (= N524), E490 (≠ G526)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V408 (≠ A444), G409 (= G445), Q410 (≠ T446), H411 (= H452), G434 (≠ S470), M436 (= M472), G460 (= G496), D461 (= D497), A462 (≠ G498), S463 (= S499), M466 (= M502), N488 (= N524), E490 (≠ G526), Q491 (≠ Y527), G492 (= G528), M493 (≠ C529), V494 (≠ I530)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V408 (≠ A444), G409 (= G445), Q410 (≠ T446), H411 (= H452), G434 (≠ S470), M436 (= M472), G460 (= G496), D461 (= D497), A462 (≠ G498), S463 (= S499), M466 (= M502), N488 (= N524), E490 (≠ G526), Q491 (≠ Y527), G492 (= G528), M493 (≠ C529), V494 (≠ I530)
Sites not aligning to the query:
6deoA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide iodomuron methyl (see paper)
25% identity, 72% coverage: 59:534/657 of query aligns to 25:493/593 of 6deoA
- active site: Y31 (≠ H65), G33 (vs. gap), G34 (= G66), A35 (≠ N67), I36 (≠ V68), E57 (= E90), T80 (= T113), F119 (≠ L154), Q120 (= Q155), E121 (≠ Q156), K169 (≠ Q213), K224 (≠ D272), M260 (≠ V308), V287 (≠ T335), V403 (≠ A444), L428 (≠ Y469), G429 (≠ S470), M431 (= M472), D456 (= D497), N483 (= N524), E485 (≠ G526), Q486 (≠ Y527), M488 (≠ C529), V489 (≠ I530), W492 (≠ L533)
- binding flavin-adenine dinucleotide: R159 (≠ L203), G213 (= G262), A214 (≠ G263), G215 (= G264), N218 (≠ Y267), T240 (= T288), L241 (≠ Q289), Q242 (≠ A290), L258 (= L306), G280 (= G328), A281 (≠ T329), R282 (= R330), D284 (≠ Q332), R286 (≠ F334), V287 (≠ T335), E313 (vs. gap), I314 (≠ V350), N318 (≠ A354), D332 (= D368), V333 (≠ A369), M408 (≠ L454), G426 (≠ Y467)
- binding methyl 2-{[(4-iodo-6-methoxypyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: M260 (≠ V308), D285 (= D333), R286 (≠ F334), M488 (≠ C529), W492 (≠ L533)
- binding magnesium ion: D456 (= D497), N483 (= N524), E485 (≠ G526)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V403 (≠ A444), G404 (= G445), Q405 (≠ T446), H406 (= H452), G429 (≠ S470), M431 (= M472), G455 (= G496), D456 (= D497), A457 (≠ G498), S458 (= S499), M461 (= M502), N483 (= N524), E485 (≠ G526), Q486 (≠ Y527), G487 (= G528), M488 (≠ C529), V489 (≠ I530)
Sites not aligning to the query:
P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
24% identity, 78% coverage: 89:599/657 of query aligns to 140:636/667 of P09342
- C161 (≠ M110) modified: Disulfide link with 307
- P194 (≠ F145) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
- C307 (≠ L265) modified: Disulfide link with 161
P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
24% identity, 78% coverage: 89:599/657 of query aligns to 137:633/664 of P09114
- P191 (≠ F145) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
- W568 (≠ L533) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.
6lpiB Crystal structure of ahas holo-enzyme (see paper)
25% identity, 77% coverage: 28:534/657 of query aligns to 5:465/539 of 6lpiB
- active site: I27 (≠ F63), G29 (≠ H65), G30 (= G66), S31 (≠ N67), I32 (≠ V68), E53 (≠ N89), C76 (≠ S115), F115 (≠ L154), Q116 (= Q155), E117 (≠ Q156), K165 (≠ Q213), M256 (≠ V308), A283 (≠ T335), V375 (≠ A444), G401 (≠ S470), M403 (= M472), D428 (= D497), N455 (= N524), A457 (≠ G526), L458 (≠ Y527), L460 (≠ C529), V461 (≠ I530), Q464 (≠ L533)
- binding flavin-adenine dinucleotide: R155 (≠ L203), G212 (= G262), G213 (= G263), G214 (= G264), T236 (= T288), L237 (≠ Q289), M238 (≠ A290), L254 (= L306), M256 (≠ V308), H257 (≠ T309), G276 (= G328), A277 (≠ T329), R278 (= R330), D280 (≠ Q332), R282 (≠ F334), A283 (≠ T335), D300 (≠ N351), I301 (≠ A352), D319 (= D368), V320 (≠ A369), M380 (≠ L454), G398 (≠ Y467)
- binding magnesium ion: D428 (= D497), N455 (= N524)
- binding thiamine diphosphate: E53 (≠ N89), C76 (≠ S115), P79 (= P118), G376 (= G445), Q377 (≠ T446), H378 (= H452), G401 (≠ S470), M403 (= M472), G427 (= G496), D428 (= D497), G429 (= G498), S430 (= S499), M433 (= M502), N455 (= N524), A457 (≠ G526), L458 (≠ Y527), G459 (= G528), L460 (≠ C529), V461 (≠ I530)
8i01A Crystal structure of escherichia coli glyoxylate carboligase (see paper)
26% identity, 78% coverage: 85:599/657 of query aligns to 47:550/594 of 8i01A
- binding flavin-adenine dinucleotide: R155 (≠ L203), G212 (= G262), G213 (= G263), G214 (= G264), N217 (≠ Y267), T238 (= T288), L239 (≠ Q289), M240 (≠ A290), V256 (≠ L306), G257 (= G307), Q259 (vs. gap), T260 (= T309), G279 (= G328), N280 (≠ T329), R281 (= R330), A283 (≠ Q332), R285 (≠ F334), H286 (≠ T335), D303 (vs. gap), I304 (≠ V350), Q308 (≠ A354), D322 (= D368), A323 (= A369), G417 (vs. gap)
- binding magnesium ion: D447 (= D497), F452 (≠ M502), E455 (≠ S505), N474 (= N524), Y476 (≠ G526)
- binding thiamine diphosphate: G395 (≠ E450), L396 (= L451), S397 (≠ H452), L422 (≠ M472), G446 (= G496), D447 (= D497), F448 (≠ G498), D449 (≠ S499), N474 (= N524), Y476 (≠ G526), L477 (≠ Y527), G478 (= G528), L479 (≠ C529), I480 (= I530)
- binding 2,3-dimethoxy-5-methyl-1,4-benzoquinone: Q354 (≠ R401), C492 (= C537), Q494 (≠ G539)
Sites not aligning to the query:
1n0hA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorimuron ethyl (see paper)
24% identity, 83% coverage: 59:602/657 of query aligns to 25:558/599 of 1n0hA
- active site: Y31 (≠ H65), G33 (vs. gap), G34 (= G66), A35 (≠ N67), I36 (≠ V68), E57 (= E90), T80 (= T113), F119 (≠ L154), Q120 (= Q155), E121 (≠ Q156), K169 (≠ Q213), R230 (≠ D272), M266 (≠ V308), V293 (≠ T335), V409 (≠ A444), L434 (≠ Y469), G435 (≠ S470), M437 (= M472), D462 (= D497), N489 (= N524), E491 (≠ G526), Q492 (≠ Y527), M494 (≠ C529), V495 (≠ I530), W498 (≠ L533), L520 (≠ H564), G525 (= G569), L526 (≠ A570)
- binding 4-{[(4'-amino-2'-methylpyrimidin-5'-yl)methyl]amino}pent-3-enyl diphosphate: V409 (≠ A444), G410 (= G445), Q411 (≠ T446), H412 (vs. gap), G435 (≠ S470), M437 (= M472), G461 (= G496), D462 (= D497), A463 (≠ G498), S464 (= S499), M467 (= M502), N489 (= N524), E491 (≠ G526), Q492 (≠ Y527), G493 (= G528), V495 (≠ I530)
- binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: G34 (= G66), A35 (≠ N67), V109 (≠ I144), P110 (≠ F145), F119 (≠ L154), K169 (≠ Q213), M266 (≠ V308), D291 (= D333), R292 (≠ F334), V495 (≠ I530), W498 (≠ L533)
- binding flavin-adenine dinucleotide: R159 (≠ L203), G219 (= G262), A220 (≠ G263), G221 (= G264), N224 (vs. gap), T246 (= T288), L247 (≠ Q289), Q248 (≠ A290), L264 (= L306), G265 (= G307), M266 (≠ V308), H267 (≠ T309), G286 (= G328), A287 (≠ T329), R288 (= R330), D290 (≠ Q332), R292 (≠ F334), V293 (≠ T335), E319 (≠ A349), V320 (= V350), N324 (≠ A354), G337 (≠ A367), D338 (= D368), A339 (= A369), M414 (≠ L447), G432 (≠ Y467), G433 (= G468)
- binding magnesium ion: D462 (= D497), N489 (= N524), E491 (≠ G526)
- binding thiamine diphosphate: Y31 (≠ H65), E57 (= E90), P83 (= P118)
Sites not aligning to the query:
1t9cA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
24% identity, 83% coverage: 59:602/657 of query aligns to 23:555/596 of 1t9cA
- active site: Y29 (≠ H65), G31 (vs. gap), G32 (= G66), A33 (≠ N67), I34 (≠ V68), E55 (= E90), T78 (= T113), F117 (≠ L154), Q118 (= Q155), E119 (≠ Q156), K167 (≠ Q213), R227 (≠ D272), M263 (≠ V308), V290 (≠ T335), V406 (≠ A444), L431 (≠ Y469), G432 (≠ S470), M434 (= M472), D459 (= D497), N486 (= N524), E488 (≠ G526), Q489 (≠ Y527), M491 (≠ C529), V492 (≠ I530), W495 (≠ L533), L517 (≠ H564), G522 (= G569), L523 (≠ A570)
- binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G32 (= G66), V107 (≠ I144), P108 (≠ F145), F117 (≠ L154), K167 (≠ Q213), D288 (= D333), R289 (≠ F334), W495 (≠ L533)
- binding flavin-adenine dinucleotide: R157 (≠ L203), G216 (= G262), A217 (≠ G263), G218 (= G264), N221 (vs. gap), T243 (= T288), L244 (≠ Q289), Q245 (≠ A290), L261 (= L306), M263 (≠ V308), H264 (≠ T309), G283 (= G328), A284 (≠ T329), R285 (= R330), D287 (≠ Q332), R289 (≠ F334), V290 (≠ T335), E316 (≠ A349), V317 (= V350), N321 (≠ A354), G334 (≠ A367), D335 (= D368), A336 (= A369), M411 (≠ L447), G429 (≠ Y467), G430 (= G468)
- binding magnesium ion: D459 (= D497), N486 (= N524), E488 (≠ G526)
Sites not aligning to the query:
1t9aA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
24% identity, 83% coverage: 59:602/657 of query aligns to 24:556/597 of 1t9aA
- active site: Y30 (≠ H65), G32 (vs. gap), G33 (= G66), A34 (≠ N67), I35 (≠ V68), E56 (= E90), T79 (= T113), F118 (≠ L154), Q119 (= Q155), E120 (≠ Q156), K168 (≠ Q213), R228 (≠ D272), M264 (≠ V308), V291 (≠ T335), V407 (≠ A444), L432 (≠ Y469), G433 (≠ S470), M435 (= M472), D460 (= D497), N487 (= N524), E489 (≠ G526), Q490 (≠ Y527), M492 (≠ C529), V493 (≠ I530), W496 (≠ L533), L518 (≠ H564), G523 (= G569), L524 (≠ A570)
- binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: G33 (= G66), V108 (≠ I144), P109 (≠ F145), F118 (≠ L154), K168 (≠ Q213), M264 (≠ V308), D289 (= D333), R290 (≠ F334), M492 (≠ C529), V493 (≠ I530), W496 (≠ L533)
- binding flavin-adenine dinucleotide: R158 (≠ L203), G217 (= G262), A218 (≠ G263), G219 (= G264), N222 (vs. gap), T244 (= T288), L245 (≠ Q289), Q246 (≠ A290), L262 (= L306), M264 (≠ V308), H265 (≠ T309), G284 (= G328), A285 (≠ T329), R286 (= R330), D288 (≠ Q332), R290 (≠ F334), V291 (≠ T335), E317 (≠ A349), V318 (= V350), N322 (≠ A354), G335 (≠ A367), D336 (= D368), A337 (= A369), Q411 (vs. gap), M412 (≠ L447), G430 (≠ Y467), G431 (= G468)
- binding magnesium ion: D460 (= D497), N487 (= N524), E489 (≠ G526)
- binding propyl trihydrogen diphosphate: V407 (≠ A444), G408 (= G445), Q409 (≠ T446), H410 (vs. gap), M435 (= M472), G459 (= G496), D460 (= D497), A461 (≠ G498), S462 (= S499), N487 (= N524), E489 (≠ G526), Q490 (≠ Y527), G491 (= G528), M492 (≠ C529)
- binding 5-{[ethyl(methyl)amino]methyl}-2-methyl-5,6-dihydropyrimidin-4-amine: G433 (≠ S470), M435 (= M472), M465 (= M502)
Sites not aligning to the query:
1t9dA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
24% identity, 83% coverage: 59:602/657 of query aligns to 23:555/596 of 1t9dA
- active site: Y29 (≠ H65), G31 (vs. gap), G32 (= G66), A33 (≠ N67), I34 (≠ V68), E55 (= E90), T78 (= T113), F117 (≠ L154), Q118 (= Q155), E119 (≠ Q156), K167 (≠ Q213), R227 (≠ D272), M263 (≠ V308), V290 (≠ T335), V406 (≠ A444), L431 (≠ Y469), G432 (≠ S470), M434 (= M472), D459 (= D497), N486 (= N524), E488 (≠ G526), Q489 (≠ Y527), M491 (≠ C529), V492 (≠ I530), W495 (≠ L533), L517 (≠ H564), G522 (= G569), L523 (≠ A570)
- binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G32 (= G66), A33 (≠ N67), V107 (≠ I144), P108 (≠ F145), F117 (≠ L154), K167 (≠ Q213), M263 (≠ V308), D288 (= D333), R289 (≠ F334), W495 (≠ L533)
- binding flavin-adenine dinucleotide: R157 (≠ L203), G216 (= G262), A217 (≠ G263), G218 (= G264), N221 (vs. gap), T243 (= T288), L244 (≠ Q289), Q245 (≠ A290), M260 (≠ A305), L261 (= L306), H264 (≠ T309), G283 (= G328), A284 (≠ T329), R285 (= R330), D287 (≠ Q332), R289 (≠ F334), V290 (≠ T335), E316 (≠ A349), V317 (= V350), N321 (≠ A354), G334 (≠ A367), D335 (= D368), A336 (= A369), Q410 (vs. gap), M411 (≠ L447), G429 (≠ Y467), G430 (= G468)
- binding magnesium ion: D459 (= D497), N486 (= N524), E488 (≠ G526)
- binding 2,5-dimethyl-pyrimidin-4-ylamine: E55 (= E90), P81 (= P118), Q118 (= Q155), G432 (≠ S470), M434 (= M472), M464 (= M502)
Sites not aligning to the query:
7pt4B Actinobacterial 2-hydroxyacyl-coa lyase (achacl) structure in complex with a covalently bound reaction intermediate as well as products formyl-coa and acetone (see paper)
26% identity, 84% coverage: 58:610/657 of query aligns to 19:549/584 of 7pt4B
- binding magnesium ion: D446 (= D497), N473 (= N524), A475 (≠ G526)
- binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-{(1r,11r,15s,17r)-19-[(2r,3s,4r,5r)-5-(6-amino-9h-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-1,11,15,17-tetrahydroxy-12,12-dimethyl-15,17-dioxido-6,10-dioxo-14,16,18-trioxa-2-thia-5,9-diaza-15,17-diphosphanonadec-1-yl}-5-(2-{[(r)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: S257 (≠ A305), R259 (≠ A314), S260 (≠ N315), Q279 (≠ D333), Y352 (vs. gap), G395 (≠ L451), G396 (≠ H452), D397 (≠ K453), L398 (= L454), L399 (≠ W455), R403 (≠ K459), L404 (≠ P460), G419 (≠ S470), L421 (≠ M472), G445 (= G496), D446 (= D497), G447 (= G498), A448 (≠ S499), N473 (= N524), A475 (≠ G526), W476 (≠ Y527), N477 (≠ G528), I478 (= I530), E479 (≠ N531), D547 (= D608)
Sites not aligning to the query:
- binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-{(1r,11r,15s,17r)-19-[(2r,3s,4r,5r)-5-(6-amino-9h-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-1,11,15,17-tetrahydroxy-12,12-dimethyl-15,17-dioxido-6,10-dioxo-14,16,18-trioxa-2-thia-5,9-diaza-15,17-diphosphanonadec-1-yl}-5-(2-{[(r)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: 561
1t9bB Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
24% identity, 83% coverage: 59:602/657 of query aligns to 23:554/595 of 1t9bB
- active site: Y29 (≠ H65), G31 (vs. gap), G32 (= G66), A33 (≠ N67), I34 (≠ V68), E55 (= E90), T78 (= T113), F117 (≠ L154), Q118 (= Q155), E119 (≠ Q156), K167 (≠ Q213), R226 (≠ D272), M262 (≠ V308), V289 (≠ T335), V405 (≠ A444), L430 (≠ Y469), G431 (≠ S470), M433 (= M472), D458 (= D497), N485 (= N524), E487 (≠ G526), Q488 (≠ Y527), M490 (≠ C529), V491 (≠ I530), W494 (≠ L533), L516 (≠ H564), G521 (= G569), L522 (≠ A570)
- binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: V107 (≠ I144), P108 (≠ F145), D287 (= D333), R288 (≠ F334), M490 (≠ C529), W494 (≠ L533)
- binding flavin-adenine dinucleotide: R157 (≠ L203), G215 (= G262), A216 (≠ G263), G217 (= G264), N220 (vs. gap), T242 (= T288), L243 (≠ Q289), Q244 (≠ A290), M259 (≠ A305), L260 (= L306), M262 (≠ V308), H263 (≠ T309), G282 (= G328), A283 (≠ T329), R284 (= R330), D286 (≠ Q332), R288 (≠ F334), V289 (≠ T335), E315 (≠ A349), V316 (= V350), N320 (≠ A354), G333 (≠ A367), D334 (= D368), A335 (= A369), Q409 (vs. gap), M410 (≠ L447), G428 (≠ Y467), G429 (= G468)
- binding magnesium ion: D458 (= D497), N485 (= N524), E487 (≠ G526)
Sites not aligning to the query:
1t9dB Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
23% identity, 83% coverage: 59:602/657 of query aligns to 22:541/582 of 1t9dB
- active site: Y28 (≠ H65), G30 (vs. gap), G31 (= G66), A32 (≠ N67), I33 (≠ V68), E54 (= E90), T77 (= T113), F116 (≠ L154), Q117 (= Q155), E118 (≠ Q156), K166 (≠ Q213), R213 (≠ D272), M249 (≠ V308), V276 (≠ T335), V392 (≠ A444), L417 (≠ Y469), G418 (≠ S470), M420 (= M472), D445 (= D497), N472 (= N524), E474 (≠ G526), Q475 (≠ Y527), M477 (≠ C529), V478 (≠ I530), W481 (≠ L533), L503 (≠ H564), G508 (= G569), L509 (≠ A570)
- binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G31 (= G66), A32 (≠ N67), V106 (≠ I144), P107 (≠ F145), F116 (≠ L154), K166 (≠ Q213), M249 (≠ V308), D274 (= D333), R275 (≠ F334), W481 (≠ L533)
- binding flavin-adenine dinucleotide: R156 (≠ L203), G202 (= G262), A203 (≠ G263), G204 (= G264), N207 (vs. gap), T229 (= T288), L230 (≠ Q289), Q231 (≠ A290), L247 (= L306), M249 (≠ V308), H250 (≠ T309), G269 (= G328), A270 (≠ T329), R271 (= R330), D273 (≠ Q332), R275 (≠ F334), V276 (≠ T335), E302 (≠ A349), V303 (= V350), N307 (≠ A354), G320 (≠ A367), D321 (= D368), A322 (= A369), Q396 (vs. gap), M397 (≠ L447), G415 (≠ Y467), G416 (= G468)
- binding magnesium ion: D445 (= D497), N472 (= N524), E474 (≠ G526)
- binding 2,5-dimethyl-pyrimidin-4-ylamine: E54 (= E90), P80 (= P118), G418 (≠ S470), M420 (= M472), M450 (= M502)
Sites not aligning to the query:
P0DUV9 2-hydroxyacyl-CoA lyase; AcHACL; HACL; 2-hydroxyisobutyryl-CoA lyase; EC 4.1.-.- from Actinomycetospora chiangmaiensis (strain DSM 45062 / JCM 15998 / CCTCC AA 205017 / NBRC 104400 / YIM 0006) (see paper)
26% identity, 84% coverage: 58:610/657 of query aligns to 33:563/590 of P0DUV9
- G43 (= G66) binding
- Q255 (= Q289) binding
- RS 273:274 (≠ AN 314:315) binding
- R362 (≠ Q412) binding
- GDL 410:412 (≠ HKL 452:454) binding
- R417 (≠ K459) binding
- G433 (≠ S470) binding
- D460 (= D497) binding
- GA 461:462 (≠ GS 498:499) binding
- N487 (= N524) binding
- NRAWN-I 487:492 (≠ NRGYGCI 524:530) binding
- A489 (≠ G526) binding
- E493 (≠ N531) mutation to A: 10-fold decrease of 2-HIB-CoA cleavage rate, 6-fold increase in KM.; mutation to K: No cleavage of 2-HIB-CoA.; mutation to Q: 50-fold decrease of 2-HIB-CoA cleavage rate, 1.5-fold increase in KM.
Sites not aligning to the query:
- 561:564 binding
- 566:590 C-terminal lid
1t9bA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
23% identity, 83% coverage: 59:602/657 of query aligns to 23:542/583 of 1t9bA
- active site: Y29 (≠ H65), G31 (vs. gap), G32 (= G66), A33 (≠ N67), I34 (≠ V68), E55 (= E90), T78 (= T113), F117 (≠ L154), Q118 (= Q155), E119 (≠ Q156), K167 (≠ Q213), R214 (≠ D272), M250 (≠ V308), V277 (≠ T335), V393 (≠ A444), L418 (≠ Y469), G419 (≠ S470), M421 (= M472), D446 (= D497), N473 (= N524), E475 (≠ G526), Q476 (≠ Y527), M478 (≠ C529), V479 (≠ I530), W482 (≠ L533), L504 (≠ H564), G509 (= G569), L510 (≠ A570)
- binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: V107 (≠ I144), P108 (≠ F145), F117 (≠ L154), D275 (= D333), R276 (≠ F334), M478 (≠ C529), W482 (≠ L533)
- binding flavin-adenine dinucleotide: R157 (≠ L203), G203 (= G262), A204 (≠ G263), G205 (= G264), N208 (vs. gap), T230 (= T288), L231 (≠ Q289), Q232 (≠ A290), M247 (≠ A305), L248 (= L306), M250 (≠ V308), H251 (≠ T309), G270 (= G328), A271 (≠ T329), R272 (= R330), D274 (≠ Q332), R276 (≠ F334), V277 (≠ T335), E303 (≠ A349), V304 (= V350), N308 (≠ A354), D322 (= D368), A323 (= A369), Q397 (vs. gap), M398 (≠ L447), G416 (≠ Y467), G417 (= G468)
- binding magnesium ion: D446 (= D497), N473 (= N524), E475 (≠ G526)
Sites not aligning to the query:
Query Sequence
>BPHYT_RS13915 FitnessBrowser__BFirm:BPHYT_RS13915
MNQRVVHHEAASANDASHASTRTDGTVRLTTAQALVRYLAAQRVATEDGEGTEPLFGGVF
AIFGHGNVAGIGEALYQHREELPTLRAHNEQAMAHSAIAYAKAHFRRRMMAVTTSIGPGA
TNLVTAAALAHVNRLPVLLLPGDIFVSRAPDPVLQQVEDFHDGGVSANDALKPVSRYFDR
IVHPAQLLNALPRAVRVLTDAALCGPVTLALPQDVQAQAWDFPVDFFKPRVVTFYSPAPR
ADEIEAAIARLRHAKRPLIVAGGGLLYGRATDALHRFATTHGIPVAETQAGKGALAWNDP
LNAGALGVTGSPAANALAHDADCVLAIGTRLQDFTTGSNTLFTQADVIAVNANAFDGLKQ
RAQVVEADARLALEALAEPLQGWHADRAWTARAHKLAASWRDTVSTLTHAPQRDTVLPYE
GDVIGAVQRSSASSPTNDIVVCAAGTLPAELHKLWRAGKPGAYHVEYGYSCMGYEIAGGL
GVKLARPAREVIVMVGDGSYLMMNSEIATSVMIGAKLIVVVLDNRGYGCINRLQQACGGA
PFNNLLENCMQGPLGAPKIDFAAHARALGAQAEHAANVAELAAALQRARAADRTYVISID
TDPAHTTDEGGWWWEVAVPEVSTRPAVRDARAKYETQLAARAEPAGSAQNTGSTDNE
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory