SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS14990 FitnessBrowser__BFirm:BPHYT_RS14990 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6vw7D Formate dehydrogenase fdsabg subcomplex fdsbg from c. Necator - nadh bound (see paper)
71% identity, 98% coverage: 1:510/520 of query aligns to 4:513/514 of 6vw7D

query
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6vw7D

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binding flavin mononucleotide: G154 (= G151), G156 (= G153), K165 (= K162), N182 (= N179), E185 (= E182), G273 (= G270), E274 (= E271), E275 (= E272), N309 (= N306), N310 (= N307), S313 (≠ T310), A490 (= A487), M491 (= M488)
binding 1,4-dihydronicotinamide adenine dinucleotide: A158 (= A155), F160 (= F157), K165 (= K162), T168 (= T165), E275 (= E272), L295 (≠ V292)
binding iron/sulfur cluster: V271 (= V268), V289 (= V286), S442 (= S439), C443 (= C440), G444 (= G441), K445 (= K442), C446 (= C443), C449 (= C446), L488 (= L485), C489 (= C486), M491 (= M488), G492 (= G489)

6tg9B Cryo-em structure of nadh reduced form of NAD+-dependent formate dehydrogenase from rhodobacter capsulatus (see paper)
59% identity, 97% coverage: 3:507/520 of query aligns to 2:492/493 of 6tg9B

query
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6tg9B

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binding flavin mononucleotide: G146 (= G151), R147 (= R152), G148 (= G153), N174 (= N179), D176 (= D181), E177 (= E182), Y254 (= Y267), G257 (= G270), E258 (= E271), N293 (= N306), N294 (= N307), S297 (≠ T310)
binding 1,4-dihydronicotinamide adenine dinucleotide: F152 (= F157), K157 (= K162), E258 (= E271), E259 (= E272), L279 (≠ V292), K466 (≠ V481), L470 (= L485)
binding iron/sulfur cluster: S426 (= S439), C427 (= C440), G428 (= G441), T429 (≠ K442), C430 (= C443), C433 (= C446), L470 (= L485), C471 (= C486), G474 (= G489)

8oh5B Cryo-em structure of the electron bifurcating transhydrogenase stnabc complex from sporomusa ovata (state 2) (see paper)
43% identity, 94% coverage: 18:507/520 of query aligns to 21:509/584 of 8oh5B

query
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8oh5B

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D

A

A

R

K

A

Q

A

R

G

N

W

L

L

L

G

G

D

K

R

N

V

I

L

M

G

G

S

T

A

N

H

F

R

S

F

F

E

D

L

M

F

K

V

I

A

K

K

K

G

G

A

A

G

G

A

A

Y
x
F

V

V

C

C

G

G

E
|
E

T

T

A

A

L

L

L

I

E

A

S

S

L

L

E

E

G

G

K

E

R

R

G

G

I

M

V
x
P

R

R

A

L

K
|
K

P

P

V
x
F

P

P

A

A

L

Q

V

S

G

G

L

F

F

W

G

G

Q

K

P

P

T

T

V

N

I
|
I

N
|
N

V

V

I

E

T

T

L

F

A

A

T

N

V

V

P

P

I

W

I

I

F

M

A

Y

R

N

G

G

A

G

A

S

F

A

Y

Y

K

A

D

A

F

Y

G

G

M

T

G

E

R

K

S

S

R

K

G

G

T

T

L

K

P

V

F

F

Q

A

L

L

A

A

G

G

N

K

V

I

K

K

Q

N

G

G

L

L

V

V

E

E

L

V

A

P

F

M

G

G

V

M

T

S

L

L

R

R

E

E

L

V

L

I

Y

Y

D

D

Y

I

G

G

T

I

A

L

S

N

G

D

R

R

P

E

A

F

R

K

A

A

V

V

Q

Q

V

M

G

G

P

P

L

S

G

G

T

G

Y

C

L

I

P

P

E

K

S

Q

Q

L

W

L

D

D

I

T

P

P

M

V

D

D

Y

Y

E

D

A

S

Y

I

A

N

A

K

V

T

G

G

A

A

V

I

V

M

G
|
G

H

S

G

G

L

M

V

I

V

V

H

M

D

D

D

E

T

T

S

T

N
x
C

L

M

A

V

E

D

L

M

A

A

Q

R

Y

F

A

F

M

L

H

D

F

F

C

T

A

V

L

K

E

E

S

S

C
|
C

G
|
G

K

K

C
|
C

T

I

P

Y

C
|
C

R

R

I

I

G

G

S

T

T

K

R

R

G

M

V

L

E

E

V

I

I

L

G

E

R

R

I

I

R

T

N

T

G

G

D

E

T

G

S

R

T

E

R

G

Q

D

V

I

Q

E

L

E

L

L

R

E

D

E

L

L

C

S

D

I

T

S

M

I

V

K

S

D

G

G

S

S

L

L

C
|
C

A
x
G

M
x
L

G
|
G

G

Q

M

T

T

A

P

P

F

N

P

P

V

V

L

L

S

T

A

T

L

I

D

R

H

Y

F

F

P

R

E

D

D

E

F

Y

binding fe2/s2 (inorganic) cluster: C41 (≠ S42), C45 (≠ L46)
binding flavin mononucleotide: R154 (= R152), K164 (= K162), N181 (= N179), F269 (≠ Y267), E273 (= E271), E274 (= E272), I307 (= I305), N308 (= N306), N309 (= N307), G489 (≠ A487), L490 (≠ M488)
binding nicotinamide-adenine-dinucleotide: G155 (= G153), G156 (= G154), F159 (= F157), F163 (≠ I161), E273 (= E271), E274 (= E272), K291 (= K289), F294 (≠ V292), G413 (= G411)
binding iron/sulfur cluster: P288 (≠ V286), C442 (= C440), G443 (= G441), C445 (= C443), C448 (= C446), C488 (= C486), L490 (≠ M488), G491 (= G489)
binding zinc ion: C425 (≠ N423)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 10
binding iron/sulfur cluster: 531, 536, 540, 542, 546, 547, 548, 559, 566, 567, 568, 569, 572
binding zinc ion: 512, 518, 523

8a6tB Cryo-em structure of the electron bifurcating fe-fe hydrogenase hydabc complex from thermoanaerobacter kivui in the reduced state (see paper)
45% identity, 80% coverage: 93:507/520 of query aligns to 141:555/630 of 8a6tB

query
sites
8a6tB

D

D

A

E

I

N

P

P

Y

F

L

Y

K

A

K

K

Q

Q

Q

T

R

R

L

L

T

V

F

L

A

K

R

H

I

C

G

G

I

T

T

V

D

D

P

P

L

A

S

S

I

V

D

Y

D

D

Y

Y

V

I

A

A

H

E

G

G

L

Y

E

S

G

A

L

I

R

A

N

K

A

A

L

L

Q

T

T

M

D

D

G

R

D

K

A

Q

A

I

C

I

E

D

A

E

L

V

I

I

E

K

S

S

G

G

L

L

R

R

G

G

R

R

G
|
G

G

G

A

A

A

G

F

F

P

P

A

T

G

G

I

E

K

K

W

W

R

L

T

G

V

A

R

Y

G

K

A

N

K

Q

A

S

A

P

Q

K

K

K

Y

Y

I

I

V

I

C

C

N
|
N

A

G

D

D

E
|
E

G

G

D

D

S

P

G

G

T

A

F

F

S

M

D

D

R

R

L

S

V

V

M

M

E

E

S

G

D

D

P

P

Y

H

V

K

L

V

I

I

E

E

G

G

M

M

I

M

I

I

A

G

G

A

V

Y

V

A

T

I

G

G

A

S

T

D

V

E

G

G

H

Y

I

I

Y

Y

V

V

R

R

S

A

E

E

Y

Y

P

P

H

L

S

A

I

V

A

Q

T

M

L

L

E

R

A

K

A

A

I

I

A

E

K

E

A

C

R

E

A

K

A

L

G

G

W

L

L

L

G

G

D

D

R

N

V

I

L

L

G

G

S

T

A

G

H

F

R

S

F

F

E

R

L

L

F

H

V

V

A

R

K

E

G

G

A

A

G

G

A

A

Y

F

V

V

C

C

G

G

E

E

E
x
S

T

T

A

A

L

L

L

T

E

Y

S

S

L

I

E

E

G

G

K

K

R

R

G

G

I

M

V

P

R

R

A

V

K
x
R

P

P

V
x
R

P
x
T

A
x
N

L

E

V

C

G

G

L

L

F

W

G

E

Q

M

P

P

T

T

V

V

I

L

N

N

N
|
N

V

V

I

E

T

T

L

F

A

A

T

C

V

I

P

P

I

E

I

I

F

I

A

L

R

N

G

G

A

G

A

E

F

W

Y

F

K

A

D

S

F

I

G

G

M

T

G

P

R

T

S

S

R

T

G

G

T

T

L

K

P

I

F

F

Q

A

L

L

A

S

G

G

N

K

V

V

K

N

Q

R

G

T

G

G

L

L

V

V

E

E

L

V

A

P

F

M

G

G

V

L

T

K

L

L

R

R

E

E

L

L

L

I

Y

F

D

D

Y

I

G

G

T

I

A

A

S

N

G

N

R

K

P

K

A

F

R

K

A

A

V

V

Q

Q

V

L

G

G

P

P

L
x
S

G

G

T

G

Y

C

L

V

P

P

E

E

S

S

Q

Q

W

L

D

D

I

L

P

P

M

I

D

D

Y

F

E

D

A

S

Y

L

A

S

A

K

V

A

G

G

A

A

V

I

V

M

G

G

H

S

G

G

L

V

V

V

H

V

D

D

D

E

T

D

S

T

N
x
C

L

M

A

V

E

D

L

F

A

A

Q

K

Y

F

A

F

M

T

H

N

F

F

C

I

A

V

L

E

E

E

S
|
S

C
|
C

G
|
G

K

K

C
|
C

T

I

P

P

C
|
C

R

R

I

E

G

G

S

N

T

K

R

K

G

M

V

L

E

E

V

I

I

L

G

E

R

R

I

I

R

T

N

E

G

G

D

K

T

G

S

K

T

E

R

G

Q

D

V

I

Q

E

L

L

R

E

D

E

L

L

C

G

D

D

T

V

M

I

V

I

S

S

G

A

S

S

L

L

C
|
C

A
x
G

M
x
L

G
|
G

G

K

M

T

T

A

P

P

F

N

P

P

V

V

L

L

S

S

A

T

L

I

D

K

H

H

F

F

P

R

E

D

D

E

F

Y

binding flavin mononucleotide: G201 (= G153), N227 (= N179), E230 (= E182), N355 (= N307), G535 (≠ A487), L536 (≠ M488)
binding nadp nicotinamide-adenine-dinucleotide phosphate: S320 (≠ E272), R337 (≠ K289), R340 (≠ V292), T341 (≠ P293), N342 (≠ A294), S433 (≠ L385)
binding iron/sulfur cluster: S487 (= S439), C488 (= C440), G489 (= G441), C491 (= C443), C494 (= C446), C534 (= C486), L536 (≠ M488), G537 (= G489)
binding zinc ion: C471 (≠ N423)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 31, 33, 36, 82, 85, 86
binding iron/sulfur cluster: 575, 577, 582, 583, 585, 588, 592, 596, 597, 607, 612, 618, 622, 624, 626, 627
binding zinc ion: 558, 564

7p8nB Tmhydabc- t. Maritima hydrogenase with bridge closed (see paper)
39% identity, 99% coverage: 4:517/520 of query aligns to 28:550/613 of 7p8nB

query
sites
7p8nB

I

I

Y

Y

V

V

P
x
C

R

V

D
x
G

S
x
T

S

G

A
x
C

L

T

A

A

L

K

G

G

A

A

D

L

A

K

L

V

A

Y

Q

S

A

A

I

F

E

E

N

E

E

E

A

L

A

K

R

K

R

R

G

N

L

L

-

L

A

K

I

V

E

T

L

L

V

N

R

R

N

T

G

G

S
x
C

R
x
C

G
|
G

L
x
R

L
x
C

W

S

L

S

E

G

P

P

L

L

V

V

E

K

V

I

Q

M

T

P

S

Y

E

-

G

-

R

R

I

F

G

F

Y

Y

A

S

N

N

I

V

E

A

A

P

G

E

D

D

V

V

S

P

A

E

L

I

F

V

D

D

A

R

G

T

F

V

L

L

E

K

G

G

G

E

A

P

-

I

-

E

-

R

-

L

-

F

-

L

-

T

-

D

-

P

-

L

H

T

A

G

N

E

R

K

V

V

G

P

V

R

V

I

D

E

A

D

I

T

P

T

Y

L

L

F

K

K

K

N

Q

Q

Q

D

R

F

L

Y

T

I

F

M

A

E

R

A

I

I

G

G

I

E

T

S

D

E

P

C

L

D

S

S

I

I

D

E

D

D

Y

Y

V

I

A

A

H

R

G

S

G

G

L

Y

E

E

G

S

L

L

R

V

N

K

A

A

L

L

Q

T

T

S

-

M

D

T

G

P

D

E

A

E

A

I

C

I

E

E

A

T

L

V

I

K

E

A

S

S

G

G

L

L

R

R

G
|
G

R
|
R

G
|
G

G

G

A

G

F

F

P

P

A

T

G

G

I

L

K

K

W

W

R

E

T

F

V

T

R

R

G

K

A

A

K

Q

A

G

A

D

Q

I

K

K

Y

F

I

V

V

V

C

C

N
|
N

A

G

D
|
D

E
|
E

G
|
G

D

D

S

P

G

G

T

A

F

F

S

M

D

N

R

R

L

T

V

L

M

L

E

E

S

R

D

D

P

P

Y

H

V

L

L

V

I

L

E

E

G

G

M

M

I

I

A

A

G

G

V

Y

V

A

T

V

G

G

A

A

T

Q

V

K

G

G

H

Y

I

A

Y

Y

V

I

R

R

S

A

E

E

Y

Y

P

P

H

F

S

A

I

V

A

K

T

M

L

F

E

K

A

K

A

A

I

I

A

E

K

D

A

A

R

R

A

K

A

L

G

G

W

L

L

L

G

G

D

E

R

N

V

I

L

L

G

G

S

T

A

G

H

F

R

S

F

F

E

D

L

L

F

E

V

V

A

K

K

E

G

G

A

A

G

G

A

A

Y
x
F

V

V

C

C

G
|
G

E
|
E

T

T

A

A

L

L

E

A

S

S

L

I

E

E

G

G

K

K

R

R

G

G

I

M

V
x
P

R

R

A

P

K

K

P

P

V

F

P

P

A

A

L

Q

V

S

G

G

L

L

F

W

G

G

Q

K

P

P

T

T

V

L

I

I

N
|
N

V

V

I

E

T

T

L

Y

A

A

T

N

V

I

P

P

I

R

I

I

F

L

A

R

R

D

G

G

A

V

A

E

F

N

Y

Y

K

R

D

K

F

R

G

G

M

T

G

E

R

N

S

S

R

P

G

G

T

T

L

K

P

M

F

F

Q

S

L

V

A

A

G

G

N

P

V

L

K

K

Q

A

G

T

G

G

L

I

V

I

E

E

L

V

A

E

F

F

G

G

V

T

T

T

L

L

R

R

E

D

L

I

Y

Y

D

N

Y

I

G

C

G

G

T

F

A

V

S

E

G

G

R

E

P

E

A

F

R

K

A

A

V

V

Q

Q

V

I

G

G

P

P

L

S

G

G

T

A

Y

C

L

L

P

S

E

E

S

D

Q

F

W

I

D

D

I

M

P

P

M

L

D

D

Y

Y

E

D

A

T

Y

L

A

K

V

A

G

D

A

A

V

M

V

V

G

G

H

S

G

G

L

I

V

V

H

I

D

T

D

K

T

K

S

T

N
x
C

L

M

A

V

E

E

L

V

A

A

Q

R

Y

F

A

F

M

L

H

D

F

F

C

T

A

K

L

R

E

E

S
|
S

C
|
C

G
|
G

K
|
K

C
|
C

T

V

P

P

C
|
C

R

R

I

E

G

G

S

T

T

M

R

Q

G

A

V

Y

E

N

V

I

I

L

G

E

R

K

I

F

R

T

N

H

G

G

D

K

T

A

S

T

T

Y

R

E

Q

D

V

L

Q

K

L

T

L

L

R

E

D

H

L

L

C

S

D

K

T

T

M

I

V

K

S

T

G

A

S

S

L
|
L

C
|
C

A
x
G

M
x
L

G
|
G

G

K

M

T

T

A

P

P

F

N

P

P

V

I

L

L

S

S

A

T

L

L

D

K

H

L

F

F

P

R

E

E

D

E

F

Y

G

-

L

I

A

A

D
x
H

V

I

S

E

H

G

H

E

T
x
C

P

P

binding fe2/s2 (inorganic) cluster: C31 (≠ P7), G33 (≠ D9), T34 (≠ S10), C36 (≠ A12), C67 (≠ S42), C68 (≠ R43), G69 (= G44), R70 (≠ L45), C71 (≠ L46)
binding flavin mononucleotide: G185 (= G151), R186 (= R152), G187 (= G153), N213 (= N179), D215 (= D181), E216 (= E182), G217 (= G183), F301 (≠ Y267), G304 (= G270), E305 (= E271), E306 (= E272), N340 (= N306), N341 (= N307), G521 (≠ A487), L522 (≠ M488)
binding iron/sulfur cluster: P320 (≠ V286), S473 (= S439), C474 (= C440), G475 (= G441), K476 (= K442), C477 (= C443), C480 (= C446), L519 (= L485), C520 (= C486), L522 (≠ M488), G523 (= G489)
binding zinc ion: C457 (≠ N423), H544 (≠ D511), C549 (≠ T516)

Sites not aligning to the query:

binding iron/sulfur cluster: 560, 567, 570, 571, 573, 577, 590, 592, 597, 599, 600, 601, 603, 607
binding zinc ion: 554

7q4vB Electron bifurcating hydrogenase - hydabc from a. Woodii (see paper)
38% identity, 97% coverage: 2:507/520 of query aligns to 6:524/599 of 7q4vB

query
sites
7q4vB

T

S

R

Q

I

I

Y

L

V

I

P
x
C

R

G

D

G

S

T

S

G

A
x
C

L

T

A

S

L

S

G

G

A

S

D

M

A

V

L

L

A

V

Q

K

A

E

I

L

E

K

N

K

E

E

A

L

A

V

R

K

R

H

G

D

L

I

-

L

-

D

A

E

I

V

E

E

L

V

V

V

R

T

N

T

G

G

S
x
C

R
x
F

G

G

L
|
L

L
x
C

W

E

L

L

E

G

P

P

L

V

V

V

E

-

V

I

Q

V

T

Y

S

P

E

E

G

G

R

T

I

F

G

-

Y

Y

A

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N

R

I

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E

E

A

A

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A

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A

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E

A

E

G

H

F

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L

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E

K

G

G

-

R

-

P

-

L

-

D

-

R

-

L

-

I

-

Y

-

N

-

E

-

K

-

G

-

D

G

G

A

H

H

H

A

P

N

L

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I

G

-

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D

N

A

E

I

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P

G

Y

F

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F

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K

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A

F

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A

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I

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L

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E

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G

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L

F

F

G

G

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P

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T

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I

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N

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N
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N

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V

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A

A

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F

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A

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A

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N

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C

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P

E

E

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S

Q

L

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L

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D

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P

E

M

I

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D

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Y

E

D

A

N

Y

L

A

I

A

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A

G

G

A

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M

G

G

H

S

G

G

L

L

V

I

V

V

H

M

D

D

D

E

T

D

S

N

N
x
C

L

M

A

V

E

D

L

V

A

A

Q

R

Y

F

A

F

M

L

H

D

F

F

C

T

A

Q

L

D

E

E

S

S

C
|
C

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G

K
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K

C
|
C

T

P

P

P

C
|
C

R

R

I

I

G

G

S

T

T

K

R

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G

M

V

L

E

E

V

I

I

L

G

E

R

R

I

I

R

C

N

D

G

G

D

K

T

G

S

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T

E

R

G

Q

D

V

I

Q

E

L

R

L

L

R

E

D

E

L

L

C

A

D

V

T

G

M

I

V

K

S

S

G

S

S

A

L

L

C
|
C

A

G

M

L

G
|
G

G

Q

M

T

T

A

P

P

F

N

P

P

V

V

L

L

S

S

A

T

L

I

D

R

H

F

F

F

P

R

E

D

D

E

F

Y

binding fe2/s2 (inorganic) cluster: C11 (≠ P7), C16 (≠ A12), C48 (≠ S42), F49 (≠ R43), L51 (= L45), C52 (≠ L46)
binding flavin mononucleotide: G166 (= G151), G168 (= G153), N196 (= N179), D198 (= D181), F284 (≠ Y267), G287 (= G270), E288 (= E271), E289 (= E272), N324 (= N307)
binding iron/sulfur cluster: C457 (= C440), G458 (= G441), K459 (= K442), C460 (= C443), C463 (= C446), C503 (= C486), G506 (= G489)
binding zinc ion: C440 (≠ N423)

Sites not aligning to the query:

binding iron/sulfur cluster: 544, 551, 552, 553, 554, 555, 556, 557, 561, 562, 574, 581, 583, 584, 585, 586, 587, 591, 593, 595
binding zinc ion: 527, 533

7t30B Structure of electron bifurcating ni-fe hydrogenase complex hydabcsl in fmn/NAD(h) bound state (see paper)
44% identity, 78% coverage: 100:507/520 of query aligns to 2:410/425 of 7t30B

query
sites
7t30B

K

K

Q

Q

Q

H

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I

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A

A

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G

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G

A

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A

A

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-

L

Q

E

T

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D

T

G

P

D

E

A

E

A

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C

L

E

E

A

E

L

V

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K

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K

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S

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G

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G
|
G

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R

G
|
G

A
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A

A

G

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|
F

P

P

A

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|
K

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W

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T
x
F

V

A

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K

G

K

A

A

K

S

A

G

A

D

Q

K

K

K

Y

Y

I

V

V

I

C

C

N
|
N

A

A

D
|
D

E
|
E

G

G

D

D

S

P

G

G

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A

F

F

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M

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D

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I

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G

G

M

M

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I

A

G

G

A

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Y

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G

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A

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G

H

Y

I

I

Y

Y

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C

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A

I

I

A

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A

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A

A

I

I

A

A

K

Q

A

A

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F

H

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L

A

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E

G

G

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A

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G

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A

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F

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C

G
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G

E

E

E
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E

T

T

A

A

L

L

L

M

E

A

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I

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E

G

G

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R

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x
F

P

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A

L

Q

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H

G

G

L

L

F

W

G

G

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K

P

P

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N

I

I

N

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|
N

V

V

I

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T

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A

A

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P

I

R

I

I

F

I

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N

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G

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F

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I

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M

I

D

D

Y

Y

E

E

A

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Y

L

A

T

A

A

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A

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G

A

A

V
x
I

V
x
M

G

G

H
x
S

G

G

L

L

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H

M

D

D

D

E

T

D

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T

N
x
C

L

M

A

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E

D

L

V

A

A

Q

K

Y

F

A

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L

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F

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E

E

S
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S

C
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C

G
|
G

K

K

C
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C

T

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P

P

C
|
C

R

R

I

E

G

G

S

T

T

K

R

Q

G

M

V

L

E

L

V

M

I

L

G

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K

I

I

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x
C

N

N

G

G

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T

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S

T

T

M

R

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Q

D

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L

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S

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K

L

L

R

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D

E

L

L

C

A

D

H

T

M

M

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K

S

E

G

T

S
|
S

L

L

C
|
C

A
x
G

M
x
L

G
|
G

G

Q

M

T

T

A

P

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F

N

P

P

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Y

F

F

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E

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Y

binding fe2/s2 (inorganic) cluster: C326 (≠ N423), C364 (≠ R461)
binding flavin mononucleotide: G54 (= G151), G56 (= G153), K65 (= K162), N82 (= N179), D84 (= D181), E85 (= E182), G173 (= G270), E175 (= E272), N210 (= N307), G390 (≠ A487), L391 (≠ M488)
binding nicotinamide-adenine-dinucleotide: G56 (= G153), G57 (= G154), A58 (= A155), F60 (= F157), K65 (= K162), F68 (≠ T165), E85 (= E182), E175 (= E272), R192 (≠ K289), F195 (≠ V292), I312 (≠ V409), M313 (≠ V410), S315 (≠ H412)
binding iron/sulfur cluster: S342 (= S439), C343 (= C440), G344 (= G441), C346 (= C443), C349 (= C446), S387 (= S484), C389 (= C486), L391 (≠ M488), G392 (= G489)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 413, 419, 421, 424

7t2rB Structure of electron bifurcating ni-fe hydrogenase complex hydabcsl in fmn-free apo state (see paper)
44% identity, 78% coverage: 100:507/520 of query aligns to 2:410/425 of 7t2rB

query
sites
7t2rB

K

K

Q

Q

Q

H

R

R

L

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V

F

L

A

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N

I

C

G

G

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I

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E

A

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A

E

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Y

I

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V

I

C

C

N

N

A

A

D

D

E

E

G

G

D

D

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P

G

G

T

A

F

F

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D

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M

L

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G

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D

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H

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I

I

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E

G

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M

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A

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Y

I

I

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Y

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C

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A

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Y

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S

A

I

I

A

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L

E

K

A

I

A

A

I

I

A

A

K

Q

A

A

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E

A

E

A

M

G

G

W

L

L

L

G

G

D

D

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A

N

H

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L

A

K

K

E

G

G

A

A

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G

A

A

Y

F

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C

C

G

G

E

E

T

T

A

A

L

L

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M

E

A

S

S

L

I

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E

G

G

K

R

R

R

G

G

I

M

V
x
P

R

R

A

P

K

R

P

P

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F

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P

A

A

L

Q

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H

G

G

L

L

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N

I

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T

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L

W

A

A

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I

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N

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G

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T

L

K

P

I

F

F

Q

A

L

L

A

T

G

G

N

K

V

I

K

T

Q

N

G

T

G

G

L

L

V

I

E

E

L

V

A

P

F

M

G

G

V

I

T

T

L

I

R

R

E

E

L

I

Y

Y

D

E

Y

L

G

G

T

I

A

L

S

N

G

G

R

K

P

E

A

F

R

K

A

A

V

V

Q

Q

V

I

G

G

P

P

L

S

G

G

T

G

Y

C

L

L

P

T

E

K

S

E

Q

H

W

L

D

D

I

L

P

P

M

I

D

D

Y

Y

E

E

A

S

Y

L

A

T

A

A

V

A

G

G

A

A

V

I

V

M

G

G

H

S

G

G

L

L

V

V

H

M

D

D

D

E

T

D

S

T

N
x
C

L

M

A

V

E
x
D

L

V

A

A

Q

K

Y

F

A

F

M

L

H

E

F

F

C

T

A

Q

L

R

E

E

S

S

C
|
C

G
|
G

K

K

C
|
C

T

V

P

P

C
|
C

R

R

I

E

G

G

S

T

T

K

R

Q

G

M

V

L

E

L

V

M

I

L

G

Q

R

K

I

I

R
x
C

N

N

G

G

D

E

T

G

S

T

T

M

R

D

Q

D

V

L

Q

S

L

K

L

L

R

E

D

E

L

L

C

A

D

H

T

M

M

V

V

K

S

E

G

T

S

S

L

L

C
|
C

A

G

M

L

G
|
G

G

Q

M

T

T

A

P

P

F

N

P

P

V

V

L

I

S

T

A

T

L

I

D

R

H

Y

F

F

P

R

E

D

D

E

F

Y

binding fe2/s2 (inorganic) cluster: C326 (≠ N423), D329 (≠ E426), C364 (≠ R461)
binding iron/sulfur cluster: P189 (≠ V286), C343 (= C440), G344 (= G441), C346 (= C443), C349 (= C446), C389 (= C486), G392 (= G489)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 413, 419, 421, 424

7q4vF Electron bifurcating hydrogenase - hydabc from a. Woodii (see paper)
44% identity, 75% coverage: 116:507/520 of query aligns to 1:395/470 of 7q4vF

query
sites
7q4vF

S

N

I

I

D

D

D

E

Y

Y

V

I

A

G

H

F

G

D

G

G

L

Y

E

L

G

A

L

L

R

E

N

K

A

V

L

L

Q

L

T

T

D

M

G

S

D

P

A

V

-

D

A

V

C

I

E

N

A

E

L

V

I

K

E

A

S

S

G

G

L

L

R

R

G
|
G

R

R

G
|
G

A

G

F
|
F

P

P

A

T

G

G

I

L

K
|
K

W

W

R

Q

T

F

V

A

R

H

G

D

A

A

K

V

A

S

A

E

Q

D

-

G

-

I

K

K

Y

Y

I

V

V

A

C

C

N
|
N

A

A

D

D

E

E

G

G

D

D

S

P

G

G

T

A

F

F

S

M

D

D

R

R

L

S

V

V

M

L

E

E

S

G

D

D

P

P

Y

H

V

A

L

V

I

I

E

E

G

A

M

M

I

A

I

I

A

A

G

G

V

Y

V

A

T

V

G

G

A

A

T

S

V

K

G

G

H

Y

I

V

Y

Y

V

V

R

R

S

A

E

E

Y

Y

P

P

H

I

S

A

I

V

A

N

T

R

L

L

E

Q

A

I

A

A

I

I

A

D

K

Q

A

A

R

K

A

E

A

Y

G

G

W

I

L

L

G

G

D

E

R

N

V

I

L

F

G

E

S

T

A

D

H

F

R

S

F

F

E

D

L

L

F

E

V

I

A

R

K

L

G

G

A

A

G

G

A

A

Y

F

V

V

C

C

G
|
G

E
|
E

T

T

A

A

L

L

L

M

E

N

S

S

L

I

E

E

G

G

K

K

R

R

G

G

I

E

V

P

R

R

A

P

K
x
R

P

P

V
x
F

P

P

A

A

L

N

V

K

G

G

L

L

F

F

G

G

Q

K

P

P

T

T

V

V

I

L

N

N

V

V

I

E

T

T

L

Y

A

A

T

N

V

I

P

P

I

K

I

I

F

I

A

L

R

N

G

G

A

A

A

E

F

W

Y

F

K

A

D

S

F

V

G

G

M

T

G

E

R

K

S

S

R

K

G

G

T

T

L

K

P

V

F

F

Q

A

L

L

A

G

G

G

N

K

V

I

K

N

Q

N

G

T

G

G

L

L

V

L

E

E

L

I

A

P

F

M

G

G

V

T

T

T

L

L

R

R

E

E

L

I

Y

Y

D

E

Y

I

G

G

T

I

A

P

S

N

G

G

R

K

P

A

A

F

R

K

A

A

V

A

Q

Q

V

T

G

G

P

P

L

S

G

G

T

G

Y

C

L

L

P

P

E

E

S

S

Q

L

W

L

D

D

I

T

P

E

M

I

D

D

Y

Y

E

D

A

N

Y

L

A

I

A

A

V

A

G

G

A

S

V
x
M

V

M

G

G

H

S

G

G

L

L

V

I

V

V

H

M

D

D

D

E

T

D

S

N

N
x
C

L

M

A

V

E

D

L

V

A

A

Q

R

Y

F

A

F

M

L

H

D

F

F

C

T

A

Q

L

D

E

E

S
|
S

C
|
C

G
|
G

K
|
K

C
|
C

T

P

P

P

C
|
C

R

R

I

I

G

G

S

T

T

K

R

R

G

M

V

L

E

E

V

I

I

L

G

E

R

R

I

I

R

C

N

D

G

G

D

K

T

G

S

V

T

E

R

G

Q

D

V

I

Q

E

L

R

L

L

R

E

D

E

L

L

C

A

D

V

T

G

M

I

V

K

S

S

G

S

S

A

L
|
L

C
|
C

A
x
G

M

L

G

G

G

Q

M

T

T

A

P

P

F

N

P

P

V

V

L

L

S

S

A

T

L

I

D

R

H

F

F

F

P

R

E

D

D

E

F

Y

binding flavin mononucleotide: G37 (= G151), G39 (= G153), N67 (= N179), G158 (= G270), E159 (= E271), E160 (= E272), G375 (≠ A487)
binding nicotinamide-adenine-dinucleotide: G40 (= G154), F43 (= F157), K48 (= K162), R177 (≠ K289), F180 (≠ V292), M297 (≠ V409)
binding iron/sulfur cluster: S327 (= S439), C328 (= C440), G329 (= G441), K330 (= K442), C331 (= C443), C334 (= C446), L373 (= L485), C374 (= C486)
binding zinc ion: C311 (≠ N423)

Sites not aligning to the query:

binding iron/sulfur cluster: 415, 417, 422, 424, 425, 426, 427, 428, 432, 433, 437, 445, 452, 454, 455, 456, 457, 458, 462, 463, 467
binding zinc ion: 398, 404, 409

8a5eB Cryo-em structure of the electron bifurcating fe-fe hydrogenase hydabc complex from acetobacterium woodii in the reduced state (see paper)
44% identity, 68% coverage: 157:507/520 of query aligns to 20:372/447 of 8a5eB

query
sites
8a5eB

F
|
F

P

P

A

T

G

G

I

L

K
|
K

W

W

R

Q

T
x
F

V

A

R

H

G

D

A

A

K

V

A

S

A

E

Q

D

-

G

-

I

K

K

Y

Y

I

V

V

A

C

C

N
|
N

A

A

D

D

E

E

G

G

D
|
D