SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS15575 FitnessBrowser__BFirm:BPHYT_RS15575 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0DPQ8 Aromatic O-demethylase, reductase subunit; NADH--hemoprotein reductase; EC 1.6.2.- from Amycolatopsis sp. (strain ATCC 39116 / 75iv2) (see paper)
37% identity, 98% coverage: 1:337/343 of query aligns to 1:334/334 of P0DPQ8

query
sites
P0DPQ8

M

M

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T

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C35 (= C37) binding
C40 (= C42) binding
C43 (= C45) binding
C75 (= C77) binding
RQYS 145:148 (≠ RSYS 148:151) binding
HVR 162:164 (≠ HIR 165:167) binding
VAT 170:172 (≠ AFT 173:175) binding
T215 (= T214) binding
F330 (= F333) binding
S334 (≠ A337) binding

5ogxA Crystal structure of amycolatopsis cytochrome p450 reductase gcob. (see paper)
37% identity, 96% coverage: 10:337/343 of query aligns to 7:333/333 of 5ogxA

query
sites
5ogxA

S

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G

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binding flavin-adenine dinucleotide: Y132 (= Y137), R144 (= R148), Q145 (≠ S149), Y146 (= Y150), S147 (= S151), H161 (= H165), V162 (≠ I166), R163 (= R167), V165 (≠ M169), G168 (= G172), V169 (≠ A173), A170 (≠ F174), T171 (= T175), T214 (= T214), E327 (≠ D331), K328 (≠ S332), F329 (= F333), T330 (= T334), P331 (≠ S335), S333 (≠ A337)
binding fe2/s2 (inorganic) cluster: N32 (≠ Y35), S33 (≠ G36), C34 (= C37), N35 (≠ K38), G37 (= G40), C39 (= C42), G40 (= G43), C42 (= C45), C74 (= C77)

7c3bC Ferredoxin reductase in carbazole 1,9a-dioxygenase (fad apo form) (see paper)
28% identity, 92% coverage: 18:333/343 of query aligns to 21:333/334 of 7c3bC

query
sites
7c3bC

Q

S

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binding fe2/s2 (inorganic) cluster: Y38 (= Y35), E39 (≠ G36), C40 (= C37), A41 (≠ K38), G43 (= G40), G44 (≠ A41), C45 (= C42), G46 (= G43), C48 (= C45), L79 (= L75), C81 (= C77)

4wqmA Structure of the toluene 4-monooxygenase nadh oxidoreductase t4mof, k270s k271s variant (see paper)
26% identity, 97% coverage: 2:333/343 of query aligns to 1:325/326 of 4wqmA

query
sites
4wqmA

A

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x
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R

Q

F

L

E

D

A

G

P

P

L

Y

G

G

T

L

F

S

F

V

L

L

R

K

E

T

D

A

S

D

D

E

K

T

P

Q

I

S

V

V

L

F

L

I

A

A

S

G

G

G

T
x
S

G

G

F

I

A

A

P

P

L

M

K

V

A

S

I

M

V

V

E

N

H

T

A

L

V

I

F

A

K

Q

N

G

L

Y

N

E

R

K

P

P

M

I

T

T

L

V

Y

F

W

Y

G

G

A

S

R

R

R

L

K

E

K

A

D

E

L

L

Y

E

L

A

E

E

L

T

A

L

E

F

Q

G

W

W

A

K

R

E

E

-

I

-

P

-

N

N

F

L

K

K

F

L

L

I

P

N

V

V

L

S

S

S

E

S

P

V

D

V

A

G

G

N

D

S

A

E

W

S

T

S

G

Y

R

P

V

T

G

G

F

Y

V

V

H

H

R

E

A

I

V

I

I

P

E

E

D

Y

L

M

P

E

D

G

L

L

S

L

A

G

Y

A

Q

E

V

F

Y

Y

A

L

C

C

G

G

A

P

P

P

V

Q

M

M

V

I

E

N

S

S

A

V

Q

Q

R

K

D

L

F

L

T

M

Q

I

H

E

H

N

G

K

L

V

P

P

E

F

D

E

E

A

F

I

Y

H

A

F

D

D

S
x
R

F
|
F

binding flavin-adenine dinucleotide: C36 (= C37), S38 (≠ N39), W57 (≠ A58), Y135 (= Y137), R146 (= R148), A147 (≠ S149), Y148 (= Y150), S149 (= S151), I162 (≠ H165), V163 (≠ I166), K164 (≠ R167), G169 (= G172), K170 (≠ A173), V171 (≠ F174), S172 (≠ T175), S209 (≠ T214), R324 (≠ S332), F325 (= F333)
binding fe2/s2 (inorganic) cluster: Y34 (= Y35), C36 (= C37), N37 (≠ K38), G39 (= G40), G40 (≠ A41), C41 (= C42), G42 (= G43), C44 (= C45), L74 (= L75), C76 (= C77)

Q03304 Toluene-4-monooxygenase system, ferredoxin--NAD(+) reductase component; T4MO; Ferredoxin--NAD(+) reductase; Toluene-4-monooxygenase systme, electron transfer component; EC 1.18.1.3 from Pseudomonas mendocina (see paper)
26% identity, 97% coverage: 3:333/343 of query aligns to 2:325/326 of Q03304

query
sites
Q03304

F

F

N

N

V

I

T

Q

L

S

R

D

Q

D

S

L

G

L

R

H

Q

H

F

F

Q

E

V

A

E

D

Q

S

D

N

E

D

P

T

V

L

L

L

S

S

A

A

L

L

R

R

Q

A

G

E

I

L

G

V

L

F

P

P

Y

Y

G

E

C
|
C

K

N

N

S

G

G

A

G

C
|
C

G

G

S

A

C
|
C

K

K

G

I

T

E

V

L

S

E

G

G

E

E

I

V

E

S

Q

N

R

L

A

W

H

P

S

D

S

A

S

P

A

G

L

L

S

A

N

A

D

R

E

E

K

L

T

R

R

K

G

N

M

R

A

F

L

L

F

A

C
|
C

C

Q

A

C

T

K

A

P

C

L

T

S

D

D

L

L

E

K

V

I

D

K

-

V

I

I

R

N

E

R

V

A

A

E

G

G

V

R

G

A

D

S

V

H

Q

P

V

P

K

K

K

R

L

F

P

S

C

T

R

R

V

V

N

V

A

S

I

K

E

R

R

F

K

L

A

S

D

D

D

E

V

M

V

F

V

E

L

L

K

R

L

L

Q

E

L

-

P

-

A

A

N

E

E

Q

R

K

L

V

Q

V

Y

F

L

S

A

P

G

G

Q

Q

Y

Y

L

F

E

M

F

V

I

D

L

V

K

P

D

E

G

L

K

G

R

T

R
|
R

S
x
A

Y
|
Y

S
|
S

M

A

A

A

N

N

A

-

P

P

H

V

T

D

E

G

G

N

P

T

I

L

E

T

L

L

H
x
I

I
x
V

R
x
K

H

A

M

V

P

P

G

N

G

G

A
x
K

F
x
V

T
x
S

D

C

H

A

V

L

F

A

N

N

T

E

M

T

K

I

E

E

R

-

D

-

I

T

L

L

R

Q

F

L

E

D

A

G

P

P

L

Y

G

G

T

L

F

S

F

V

L

L

R

K

E

T

D

A

S

D

D

E

K

T

P

Q

I

S

V

V

L

F

L

I

A

A

S

G

G

G

T

S

G

G

F

I

A

A

P

P

L

M

K

V

A

S

I

M

V

V

E

N

H

T

A

L

V

I

F

A

K

Q

N

G

L

Y

N

E

R

K

P

P

M

I

T

T

L

V

Y

F

W

Y

G

G

A

S

R

R

R

L

K

E

K

A

D

E

L

L

Y

E

L

A

E

E

L

T

A

L

E

F

Q

G

W

W

A

K

R

E

E

-

I

-

P

-

N

N

F

L

K

K

F

L

L

I

P

N

V

V

L

S

S

S

E

S

P

V

D

V

A

G

G

N

D

S

A

E

W

K

T

K

G

Y

R

P

V

T

G

G

F

Y

V

V

H

H

R

E

A

I

V

I

I

P

E

E

D

Y

L

M

P

E

D

G

L

L

S

L

A

G

Y

A

Q

E

V

F

Y

Y

A

L

C

C

G

G

A

P

P

P

V

Q

M

M

V

I

E

N

S

S

A

V

Q

Q

R

K

D

L

F

L

T

M

Q

I

H

E

H

N

G

K

L

V

P

P

E

F

D

E

E

A

F

I

Y

H

A

F

D

D

S

R

F

F

C36 (= C37) binding
C41 (= C42) binding
C44 (= C45) binding
C76 (= C77) binding
RAYS 146:149 (≠ RSYS 148:151) binding
IVK 162:164 (≠ HIR 165:167) binding
KVS 170:172 (≠ AFT 173:175) binding

1krhA X-ray structure of benzoate dioxygenase reductase (see paper)
27% identity, 93% coverage: 16:335/343 of query aligns to 19:336/337 of 1krhA

query
sites
1krhA

V

I

E

A

Q

Q

D

G

E

E

P

T

V

L

L

S

S

D

A

A

L

Y

R

R

Q

Q

G

Q

I

I

G

N

L

I

P

P

Y
x
M

G
x
D

C
|
C

K
x
R

N

E

G
|
G

A
x
E

C
|
C

G
|
G

S

T

C
|
C

K

R

G

A

T

F

V

C

V

E

S

S

G

G

-

N

-

Y

E

D

I

M

E

P

Q

E

R

D

A

N

H

Y

S

I

S

E

S

D

A

A

L

L

S

T

N

P

D

E

E

E

K

A

T

Q

R

Q

G

G

M

Y

A

V

L

L

F

A

C
|
C

C

Q

A

C

T

R

A

P

C

T

T

S

D

D

L

A

E

V

V

F

D

Q

I

I

R

Q

E

A

V

S

A

S

G

E

V

V

G

C

D

K

V

T

Q

K

V

I

K

H

L

F

P

E

C

G

R

T

V

L

N

A

A

R

I

V

E

E

R

N

K

L

A

S

D

D

D

S

V

T

V

I

V

T

L

F

K

D

L

I

Q

Q

L

L

P

D

A

D

N

G

E

Q

-

P

R

D

L

I

Q

H

Y

F

L

L

A

A

G

G

Q

Q

Y
|
Y

L

V

E

N

F

V

I

T

L

L

K

P

-

G

D

T

G

T

K

E

R

T

R
|
R

S
|
S

Y
|
Y

S
|
S

M

F

A

S

N

S

A

Q

P

P

H

G

T

N

E

R

G

-

P

L

I

T

E

G

L

F

H
x
V

I
x
V

R
|
R

H

N

M
x
V

P

P

G

Q

G
|
G

A
x
K

F
x
M

T
x
S

D

E

H

Y

V

L

F

S

N

V

T

Q

M

A

K

K

E

A

R

G

D

D

I

K

L

M

R

S

F

F

E

T

A

G

P

P

L

F

G

G

T

S

F

F

F

Y

L

L

R

R

E

-

D

D

S

V

D

K

K

R

P

P

I

V

V

L

L

M

L

L

A

A

S

G

G

G

T
|
T

G

G

F

I

A

A

P

P

L

F

K

L

A

S

I

M

V

L

E

Q

H

V

A

L

V

E

F

Q

K

K

N

G

L

S

N

E

R

H

P

P

M

V

T

R

L

L

Y

V

W

F

G

G

A

V

R

T

R

Q

K

D

K

C

D

D

L

L

Y

V

L

A

L

L

E

E

L

Q

A

L

E

D

Q

A

W

L

A

Q

R

Q

E

K

I

L

P

P

N

W

F

F

K

E

F

Y

L

R

P

T

V

V

L

V

S

A

E

H

P

A

D

E

A

S

G

Q

D

H

A

E

W

-

T

-

G

-

R

R

V

K

G

G

F

Y

V

V

H

T

R

G

A

H

V

I

I

E

E

Y

D

D

L

W

P

L

D

N

L

G

S

G

A

E

Y

V

Q

D

V

V

Y

Y

A

L

C
|
C

G

G

A

P

P

V

V

P

M

M

V

V

E

E

S

-

A

A

Q

V

R

R

D

S

F

W

T

L

Q

D

H

T

H

Q

G

G

L

I

P

Q

E

P

D

A

E

N

F

F

Y

L

A

F

D
x
E

S
x
K

F
|
F

T
x
S

S
x
A

active site: C306 (= C304)
binding flavin-adenine dinucleotide: Y143 (= Y137), R155 (= R148), S156 (= S149), Y157 (= Y150), S158 (= S151), V171 (≠ H165), V172 (≠ I166), R173 (= R167), V175 (≠ M169), G178 (= G172), K179 (≠ A173), M180 (≠ F174), S181 (≠ T175), T219 (= T214), E332 (≠ D331), K333 (≠ S332), F334 (= F333), S335 (≠ T334), A336 (≠ S335)
binding fe2/s2 (inorganic) cluster: M38 (≠ Y35), D39 (≠ G36), C40 (= C37), R41 (≠ K38), G43 (= G40), E44 (≠ A41), C45 (= C42), G46 (= G43), C48 (= C45), C82 (= C77)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 337

7c3aA Ferredoxin reductase in carbazole 1,9a-dioxygenase (see paper)
28% identity, 92% coverage: 18:333/343 of query aligns to 20:320/321 of 7c3aA

query
sites
7c3aA

Q

S

D

D

E

K

P

S

V

L

L

L

S

V

A

S

A

A

L

L

R

A

Q

N

G

G

I

I

G

G

L

L

P

P

Y
|
Y

G
x
E

C
|
C

K
x
A

N
x
S

G
|
G

A
x
G

C
|
C

G
|
G

S

V

C
|
C

K

K

G

F

T

E

V

L

S

E

G

G

E

T

I

V

E

Q

Q

S

R

M

A
x
W

H

P

S

D

S

A

S

P

A

G

L

L

S

S

N

S

D

R

E

D

K

R

T

E

R

K

G

G

-

N

M

R

A

H

L

L

F

A

C
|
C

C

Q

A

C

T

I

A

A

C

L

T

S

D

D

L

L

E

R

V

I

D

K

I

V

R

A

-

V

E

Q

V

D

A

K

G

Y

V

I

G

P

D

A

V

I

Q

P

V

I

K

S

K

K

L

M

P

E

C

A

R

E

V

V

N

V

A

A

I

V

E

R

R

A

K

L

A

T

D

H

D

D

V

L

V

S

L

V

K

K

L

L

Q

R

-

T

-

D

L

V

P

P

A

A

N

N

E

-

R

-

L

-

Q

-

Y

F

L

L

A

P

G

G

Q

Q

Y
x
F

-

C

-

L

L

I

E

E

F

A

I

E

L

Q

K

L

D

P

G
|
G

K

V

R

V

R
|
R

S
x
A

Y
|
Y

S
|
S

M

M

A

A

N

N

A

S

P

M

H

N

T

P

E

D

G

G

P

F

I

W

E

E

L

F

H
x
Y

I
|
I

R
x
K

H

R

M

V

P

P

G

T

G
|
G

A
x
R

F
|
F

T
x
S

D

P

H

W

V

L

F

F

N

E

T

N

M

R

K

K

E

V

R

G

D

A

I

R

L

L

R

F

F

L

E

T

A

G

P

P

L

M

G

G

T

T

F

S

F

F

L

F

R

R

E

P

D

G

S

T

D

G

K

R

P

K

I

S

V

L

L

C

L

I

A

G

S

G

G

G

T
x
A

G

G

F

L

A
x
S

P

Y

L

A

K

A

A

A

I

I

V

A

E

R

H

A

A

S

V

I

F

R

K

E

N

T

L

-

N

D

R

K

P

P

M

V

T

K

L

L

Y

F

W

Y

G

G

A

S

R

R

R

T

K

P

K

R

D

D

L

-

Y

-

L

-

E

-

L

-

A

A

E

V

Q

R

W

W

A

I

R

D

E

I

I

D

P

I

N

D

F

E

K

D

F

K

L

L

P

E

V

V

L

V

S

Q

E

A

P

-

D

-

A

-

G

-

D

-

A

-

W

-

T

-

G

-

R

-

V

V

G

T

F

F

V

I

H

H

R

Q

-

V

-

D

-

A

A

A

V

L

I

L

E

E

D

T

L

L

P

P

D

E

L

-

S

-

A

-

Y

Y

Q

E

V

I

Y

Y

A

L

C

A

G

G

A

P

P

P

V

P

M

M

V

V

E

D

S

A

A

T

Q

V

R

R

D

-

F

M

T

L

Q

L

H

G

H

K

G

G

L

V

P

P

E

R

D

D

E

Q

F

I

Y

H

A

F

D

D

S
x
A

F
|
F

binding flavin-adenine dinucleotide: C39 (= C37), S41 (≠ N39), W60 (≠ A58), F139 (≠ Y137), G149 (= G145), R152 (= R148), A153 (≠ S149), Y154 (= Y150), S155 (= S151), Y169 (≠ H165), I170 (= I166), K171 (≠ R167), G176 (= G172), R177 (≠ A173), F178 (= F174), S179 (≠ T175), A218 (≠ T214), S221 (≠ A217), A319 (≠ S332), F320 (= F333)
binding fe2/s2 (inorganic) cluster: Y37 (= Y35), E38 (≠ G36), C39 (= C37), A40 (≠ K38), G42 (= G40), G43 (≠ A41), C44 (= C42), G45 (= G43), C47 (= C45), C80 (= C77)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 321

7qu0A X-ray structure of fad domain of nqrf of klebsiella pneumoniae (see paper)
30% identity, 61% coverage: 100:309/343 of query aligns to 3:256/279 of 7qu0A

query
sites
7qu0A

V

V

K

K

L

W

P

E

C

C

R

E

V

V

N

I

A

S

I

N

E

D

R

N

K

K

A

A

D

T

D

F

V

I

V

K

V

E

L

L

K

K

L

L

Q

R

L

I

P

P

A

E

N

G

E

E

R

V

L

V

Q

P

Y

F

L

R

A

A

G

G

Q

G

Y
|
Y

L

I

E

Q

-

I

-

E

-

C

-

P

-

H

-

K

-

V

-

A

-

Y

-

A

-

D

F

F

I

D

L

V

K

P

D

D

G

E

K

Y

R

R

-

S

-

D

-

W

-

D

-

K

-

F

-

N

-

L

-

F

-

R

-

Y

-

V

-

S

-

E

-

V

-

K

-

E

-

P

-

T

-

L

R
|
R

S
x
A

Y
|
Y

S
|
S

M

M

A

A

N

N

A

Y

P

P

H

E

T

E

E

K

G

G

P

I

I

I

E

M

L

L

H
x
N

I
x
V

R
|
R

-

I

-
x
A

-
x
T

-
x
P

-

P

-

K

-
x
I

-

P

H

D

M
x
A

P
|
P

G
x
P

G
|
G

A
x
I

F
x
M

T
x
S

D

S

H

Y

V

I

F

W

N

-

T

S

M

L

K

K

E

P

R

G

D

D

I

K

L

V

R

T

F

I

E

S

A

G

P

P

L

F

G

G

T

E

F

F

L

A

R

K

E

E

D

-

S

T

D

D

K

A

P

E

I

M

V

V

L

F

L

I

A

G

S

G

G

G

T
x
A

G

G

F

M

A

A

P

P

L

M

K

R

A

S

-

H

I

I

V

F

E

D

H

Q

A

L

V

K

F

R

K

L

N

H

L

S

N

T

R

R

P

K

M

I

T

S

L

F

Y

W

W

Y

G
|
G

A
|
A

R
|
R

R

S

K

L

K

R

D

E

L

M

Y

F

L

Y

L

D

E

E

L

E

A

F

E

E

Q

Q

W

L

A

A

R

R

E

D

I

N

P

P

N

N

F

F

K

T

F

F

L

H

P

V

V

A

L

L

S
|
S

E

D

P

P

D

L

A

P

G

E

D

D

A

N

W

W

T

T

G

G

R

H

V

T

G

G

F
|
F

V

I

H

H

R

N

A

V

V

L

I

Y

E

E

-

N

-

Y

-

L

-

R

D

D

L

H

P

P

D

A

L

P

S

E

A

D

Y

C

Q

E

V

F

Y

Y

A

M

C

C

G

G

A
x
P

P

P

V
|
V

M
|
M

binding ~{N}-[2,6-bis(fluoranyl)phenyl]ethanamide: G183 (= G240), A184 (= A241), R185 (= R242), S213 (= S270), F227 (= F284), P253 (≠ A306), V255 (= V308), M256 (= M309)
binding flavin-adenine dinucleotide: Y40 (= Y137), R83 (= R148), A84 (≠ S149), Y85 (= Y150), S86 (= S151), N100 (≠ H165), V101 (≠ I166), R102 (= R167), A104 (vs. gap), T105 (vs. gap), P106 (vs. gap), P107 (vs. gap), I110 (vs. gap), A113 (≠ M169), P114 (= P170), P115 (≠ G171), G116 (= G172), I117 (≠ A173), M118 (≠ F174), S119 (≠ T175), A156 (≠ T214)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 279

7qtyA X-ray structure of fad domain of nqrf of klebsiella pneumoniae (see paper)
30% identity, 61% coverage: 100:309/343 of query aligns to 3:256/279 of 7qtyA

query
sites
7qtyA

V

V

K

K

L

W

P

E

C

C

R

E

V

V

N

I

A

S

I

N

E

D

R

N

K

K

A

A

D

T

D

F

V

I

V

K

V

E

L

L

K

K

L

L

Q

R

L

I

P

P

A

E

N

G

E

E

R

V

L

V

Q

P

Y

F

L

R

A

A

G

G

Q

G

Y
|
Y

L

I

E

Q

-

I

-

E

-

C

-

P

-

H

-

K

-

V

-

A

-

Y

-

A

-

D

F

F

I

D

L

V

K

P

D

D

G

E

K

Y

R

R

-

S

-

D

-

W

-

D

-

K

-

F

-

N

-

L

-

F

-

R

-

Y

-

V

-

S

-

E

-

V

-

K

-

E

-

P

-

T

-

L

R
|
R

S
x
A

Y
|
Y

S
|
S

M

M

A

A

N

N

A

Y

P

P

H

E

T

E

E

K

G

G

P

I

I

I

E

M

L

L

H
x
N

I
x
V

R
|
R

-

I

-
x
A

-
x
T

-
x
P

-

P

-

K

-
x
I

-

P

H

D

M
x
A

P
|
P

G
x
P

G
|
G

A
x
I

F
x
M

T
x
S

D

S

H

Y

V

I

F

W

N

-

T

S

M

L

K

K

E

P

R

G

D

D

I

K

L

V

R

T

F

I

E

S

A

G

P

P

L

F

G

G

T

E

F

F

L

A

R

K

E

E

D

-

S

T

D

D

K

A

P

E

I

M

V

V

L

F

L

I

A

G

S

G

G

G

T
x
A

G

G

F

M

A

A

P

P

L

M

K

R

A

S

-

H

I

I

V

F

E

D

H

Q

A

L

V

K

F

R

K

L

N

H

L

S

N

T

R

R

P

K

M

I

T

S

L

F

Y

W

W

Y

G

G

A
|
A

R
|
R

R

S

K

L

K

R

D

E

L

M

Y

F

L

Y

L

D

E

E

L

E

A

F

E

E

Q

Q

W

L

A

A

R

R

E

D

I

N

P

P

N

N

F

F

K

T

F

F

L

H

P

V

V

A

L

L

S
|
S

E

D

P

P

D

L

A

P

G

E

D

D

A

N

W

W

T

T

G

G

R

H

V

T

G

G

F
|
F

V

I

H
|
H

R

N

A

V

V

L

I

Y

E

E

-

N

-

Y

-

L

-

R

D

D

L

H

P

P

D

A

L

P

S

E

A

D

Y

C

Q

E

V

F

Y

Y

A

M

C

C

G

G

A
x
P

P

P

V
|
V

M
|
M

binding 1-(furan-2-ylmethyl)-3-(2-methylphenyl)thiourea: A184 (= A241), R185 (= R242), S213 (= S270), F227 (= F284), H229 (= H286), P253 (≠ A306), V255 (= V308), M256 (= M309)
binding flavin-adenine dinucleotide: Y40 (= Y137), R83 (= R148), A84 (≠ S149), Y85 (= Y150), S86 (= S151), N100 (≠ H165), V101 (≠ I166), R102 (= R167), A104 (vs. gap), T105 (vs. gap), P106 (vs. gap), P107 (vs. gap), I110 (vs. gap), A113 (≠ M169), P114 (= P170), P115 (≠ G171), G116 (= G172), I117 (≠ A173), M118 (≠ F174), S119 (≠ T175), A156 (≠ T214)

Sites not aligning to the query:

8a1yF Na(+)-translocating NADH-quinone reductase subunit F
27% identity, 79% coverage: 64:333/343 of query aligns to 98:406/408 of 8a1yF

query
sites
8a1yF

L

I

S

S

N

K

D

G

E

E

K

A

T

R

R

E

G

G

M

E

A

R

L

L

F

A

C
|
C

C

Q

A

V

T

A

A

V

C

K

T

A

D

D

L

M

E

D

V

L

D

E

I

L

-

P

R

E

E

E

V

I

A

F

G

G

V

V

G

-

D

-

V

-

Q

-

V

-

K

K

L

W

P

E

C

C

R

T

V

V

N

I

A

S

I

N

E

D

R

N

K

K

A

A

D

T

D

F

V

I

V

K

V

E

L

L

K

K

L

L

Q

A

L

I

P

P

A

D

N

G

E

E

R

S

L

V

Q

P

Y

F

L

R

A

A

G

G

Q

G

Y

Y

L

I

E

Q

-

I

-

E

-

A

-

P

-

A

-

H

-

V

-

K

-

Y

-

A

-

D

F

F

I

D

L

V

K

P

D

E

G

K

K

Y

R

R

-

G

-

D

-

W

-

D

-

K

-

F

-

N

-

L

-

F

-

R

-

Y

-

E

-

S

-

K

-

V

-

D

-

E

-

P

-

I

R
|
R

S
x
A

Y
|
Y

S
|
S

M

M

A

A

N

N

A

Y

P

P

H

E

T

E

E

F

G

G

P

I

I

I

E

M

L

L

H
x
N

I
x
V

R
|
R

-

I

-
x
A

-
x
T

-
x
P

-

P

-

N

-

P

H

N

M
x
V

P
|
P

G
x
P

G
|
G

A
x
Q

F
x
M

T
x
S

D

S

H

Y

V

I

F

W

N

-

T

S

M

L

K

K

E

A

R

G

D

D

I

K

L

C

R

T

F

I

E

S

A

G

P

P

L

F

G

G

T

E

F

F

L

A

R

K

E

-

D

D

S

T

D

D

K

A

P

E

I

M

V

V

L

F

L

I

A

G

S

G

G

G

T
x
A

G

G

F

M

A

A

P

P

L

M

K

R

A

S

-

H

I

I

V

F

E

D

H

Q

A

L

V

K

F

R

K

L

N

K

L

S

N

K

R

R

P

K

M

M

T

S

L

Y

Y

W

W

Y

G

G

A

A

R

R

R

S

K

K

K

R

D

E

L

M

Y

F

L

Y

L

V

E

E

L

D

A

F

E

D

Q

G

W

L

A

A

R

A

E

E

I

N

P

D

N

N

F

F

K

V

F

W

L

H

P

C

V

A

L

L

S

S

E

D

P

P

D

Q

A

P

G

E

D

D

A

N

W

W

T

T

G

G

R

Y

V

T

G

G

F

F

V

I

H

H

R

N

A

V

V

L

I

Y

E

E

D

N

-

Y

L

L

P

K

D

D

L

H

S

E

A

A

-

P

-

E

-

D

Y

C

Q

E

V

Y

Y

Y

A

M

C

C

G

G

A

P

P

P

V

-

M

M

V

M

E

N

S

A

A

A

Q

V

R

I

D

N

F

M

T

L

Q

K

H

N

H

L

G

G

L

V

P

E

E

E

D

E

E

N

F

I

Y

L

A

L

D

D

S

D

F
|
F

binding flavin-adenine dinucleotide: R210 (= R148), A211 (≠ S149), Y212 (= Y150), S213 (= S151), N227 (≠ H165), V228 (≠ I166), R229 (= R167), A231 (vs. gap), T232 (vs. gap), P233 (vs. gap), V240 (≠ M169), P241 (= P170), P242 (≠ G171), G243 (= G172), Q244 (≠ A173), M245 (≠ F174), S246 (≠ T175), A283 (≠ T214), F406 (= F333)
binding fe2/s2 (inorganic) cluster: C111 (= C77)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 70, 74, 75, 76, 77, 79

A5F5Y4 Na(+)-translocating NADH-quinone reductase subunit F; Na(+)-NQR subunit F; Na(+)-translocating NQR subunit F; NQR complex subunit F; NQR-1 subunit F; EC 7.2.1.1 from Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395) (see paper)
27% identity, 79% coverage: 64:333/343 of query aligns to 98:406/408 of A5F5Y4

query
sites
A5F5Y4

L

I

S

S

N

K

D

G

E

E

K

A

T

R

R

E

G

G

M

E

A

R

L

L

F

A

C
|
C

C

Q

A

V

T

A

A

V

C

K

T

A

D

D

L

M

E

D

V

L

D

E

I

L

-

P

R

E

E

E

V

I

A

F

G

G

V

V

G

-

D

-

V

-

Q

-

V

-

K

K

L

W

P

E

C

C

R

T

V

V

N

I

A

S

I

N

E

D

R

N

K

K

A

A

D

T

D

F

V

I

V

K

V

E

L

L

K

K

L

L

Q

A

L

I

P

P

A

D

N

G

E

E

R

S

L

V

Q

P

Y

F

L

R

A

A

G

G

Q

G

Y

Y

L

I

E

Q

-

I

-

E

-

A

-

P

-

A

-

H

-

V

-

K

-

Y

-

A

-

D

F

F

I

D

L

V

K

P

D

E

G

K

K

Y

R

R

-

G

-

D

-

W

-

D

-

K

-

F

-

N

-

L

-

F

-

R

-

Y

-

E

-

S

-

K

-

V

-

D

-

E

-

P

-

I

R
|
R

S

A

Y
|
Y

S

S

M

M

A

A

N

N

A

Y

P

P

H

E

T

E

E

F

G

G

P

I

I

I

E

M

L

L

H

N

I

V

R

R

-

I

-

A

-

T

-

P

-

N

-

P

H

N

M

V

P

P

G

P

G

G

A

Q

F

M

T
x
S

D

S

H

Y

V

I

F

W

N

-

T

S

M

L

K

K

E

A

R

G

D

D

I

K

L

C

R

T

F

I

E

S

A

G

P

P

L

F

G

G

T

E

F

F

L

A

R

K

E

-

D

D

S

T

D

D

K

A

P

E

I

M

V

V

L

F

L

I

A

G

S

G

G

G

T

A

G

G

F

M

A

A

P

P

L

M

K

R

A

S

-

H

I

I

V

F

E

D

H

Q

A

L

V

K

F

R

K

L

N

K

L

S

N

K

R

R

P

K

M

M

T

S

L

Y

Y

W

W

Y

G

G

A

A

R

R

R

S

K

K

K

R

D

E

L

M

Y

F

L

Y

L

V

E

E

L

D

A

F

E

D

Q

G

W

L

A

A

R

A

E

E

I

N

P

D

N

N

F

F

K

V

F

W

L

H

P

C

V

A

L

L

S

S

E

D

P

P

D

Q

A

P

G

E

D

D

A

N

W

W

T

T

G

G

R

Y

V

T

G

G

F

F

V

I

H

H

R

N

A

V

V

L

I

Y

E

E

D

N

-

Y

L

L

P

K

D

D

L

H

S

E

A

A

-

P

-

E

-

D

Y

C

Q

E

V

Y

Y

Y

A

M

C

C

G

G

A

P

P

P

V

-

M

M

V

M

E

N

S

A

A

A

Q

V

R

I

D

N

F

M

T

L

Q

K

H

N

H

L

G

G

L

V

P

E

E

E

D

E

E

N

F

I

Y

L

A

L

D

D

S

D

F

F

C111 (= C77) binding ; mutation to A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
R210 (= R148) mutation to L: Decreases flavin content, but does not affect the 2Fe-2S center. Exhibits very low NADH:quinone oxidoreductase activity.
Y212 (= Y150) mutation to L: Decreases flavin content, but does not affect the 2Fe-2S center. Exhibits very low NADH:quinone oxidoreductase activity.
S246 (≠ T175) mutation to A: Decreases flavin content, but does not affect the 2Fe-2S center. Exhibits very low NADH:quinone oxidoreductase activity.

Sites not aligning to the query:

70 binding ; C→A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
76 binding ; C→A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
79 binding ; C→A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.

7qu3A X-ray structure of fad domain of nqrf of pseudomonas aeruginosa (see paper)
29% identity, 61% coverage: 100:309/343 of query aligns to 3:255/279 of 7qu3A

query
sites
7qu3A

V

V

K

K

L

W

P

E

C

C

R

T

V

V

N

E

A

S

I

N

E

P

R

N

K

V

A

A

D

T

D

F

V

I

V

K

V

E

L

L

K

T

L

L

Q

R

L

L

P

P

A

D

N

G

E

E

R

S

L

V

Q

D

Y

F

L

R

A

A

G

G

-

G

-
x
Y

-

V

-

Q

-

L

-

E

-

C

-

P

-

H

-

V

Q

E

Y

Y

L

K

E

D

F

F

I

D

L

I

K

Q

D

P

G

E

K

Y

R

R

-

G

-

D

-

W

-

D

-

K

-

F

-

N

-

M

-

W

-

R

-

Y

-

V

-

S

-

K

-

V

-

D

-

E

-

T

-

V

-

I

R
|
R

S
x
A

Y
|
Y

S
|
S

M

M

A

A

N

N

A

Y

P

P

H

E

T

E

E

Q

G

G

P

V

I

V

E

K

L

F

H
x
N

I
|
I

R
|
R

-

I

-
x
A

-
x
S

-
x
P

-

P

-

G

-

S

H

D

M
x
L

P
|
P

G
x
P

G
|
G

A
x
Q

F
x
M

T
x
S

D

S

H

W

V

V

F

F

N

N

T

-

M

L

K

K

E

P

R

G

D

D

I

K

L

V

R

T

F

V

E

Y

A

G

P

P

L

F

G

G

T

E

F

F

L

A

R

K

E

-

D

D

S

T

D

E

K

A

P

E

I

M

V

V

L

F

L

I

A

G

S

G

G
|
G

T
x
A

G

G

F

M

A

A

P

P

L

M

K

R

A

S

-

H

I

I

V

F

E

D

H

Q

A

L

V

R

F

R

K

L

N

K

L

S

N

N

R

R

P

K

M

I

T

S

L

F

Y

W

W

Y

G
|
G

A
|
A

R

R

R

S

K

L

K

R

D

E

L

A

Y

F

L

Y

L

T

E

E

L

E

A

Y

E

D

Q

Q

W

L

A

Q

R

A

E

E

I

N

P

P

N

N

F

F

K

Q

F

W

L

H

P

L

V

A

L

L

S
|
S

E

D

P

P

D

Q

A

P

G

E

D

D

A

N

W

W

T

T

G

G

R

L

V

T

G

G

F
|
F

V
x
I

H
|
H

R

N

A

V

V

L

I

F

E

E

-

N

-

Y

-

L

-

K

D

D

L

H

P

P

D

A

L

P

S

E

A

D

Y

C

Q

E

V

F

Y

Y

A

M

C

C

G

G

A
x
P

P

P

V
x
M

M
|
M

binding flavin-adenine dinucleotide: Y40 (vs. gap), R83 (= R148), A84 (≠ S149), Y85 (= Y150), S86 (= S151), N100 (≠ H165), I101 (= I166), R102 (= R167), A104 (vs. gap), S105 (vs. gap), P106 (vs. gap), L112 (≠ M169), P113 (= P170), P114 (≠ G171), G115 (= G172), Q116 (≠ A173), M117 (≠ F174), S118 (≠ T175), A155 (≠ T214)
binding 4-(benzimidazol-1-ylmethyl)benzenecarbonitrile: R102 (= R167), G154 (= G213), G182 (= G240), A183 (= A241), S212 (= S270), F226 (= F284), I227 (≠ V285), H228 (= H286), P252 (≠ A306), M254 (≠ V308), M255 (= M309)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 278

7qu5A X-ray structure of fad domain of nqrf of pseudomonas aeruginosa (see paper)
29% identity, 61% coverage: 100:309/343 of query aligns to 3:255/280 of 7qu5A

query
sites
7qu5A

V

V

K

K

L

W

P

E

C

C

R

T

V

V

N

E

A

S

I

N

E

P

R

N

K

V

A

A

D

T

D
x
F

V

I

V

K

V

E

L

L

K

T

L

L

Q

R

L

L

P

P

A

D

N

G

E

E

R

S

L

V

Q

D

Y

F

L

R

A

A

G

G

-

G

-
x
Y

-

V

-

Q

-

L

-

E

-

C

-

P

-

H

-

V

Q

E

Y

Y

L

K

E

D

F

F

I

D

L

I

K

Q

D

P

G

E

K

Y

R

R

-

G

-

D

-

W

-

D

-

K

-
x
F

-

N

-
x
M

-

W

-

R

-

Y

-

V

-

S

-

K

-

V

-

D

-

E

-

T

-

V

-

I

R
|
R

S
x
A

Y
|
Y

S
|
S

M

M

A

A

N

N

A

Y

P

P

H

E

T

E

E

Q

G

G

P

V

I

V

E

K

L

F

H
x
N

I
|
I

R
|
R

-
x
I

-
x
A

-
x
S

-
x
P

-

P

-
x
P

-

G

-

S

H

D

M
x
L

P
|
P

G
x
P

G
|
G

A
x
Q

F
x
M

T
x
S

D

S

H

W

V

V

F

F

N

N

T

-

M

L

K

K

E

P

R

G

D

D

I

K

L

V

R

T

F

V

E

Y

A

G

P

P

L

F

G

G

T

E

F

F

L

A

R

K

E

-

D

D

S

T

D

E

K

A

P

E

I

M

V

V

L

F

L

I

A

G

S

G

G

G

T
x
A

G

G

F

M

A

A

P

P

L

M

K

R

A

S

-

H

I

I

V

F

E

D

H

Q

A

L

V

R

F

R

K

L

N

K

L

S

N

N

R

R

P

K

M

I

T

S

L

F

Y

W

W

Y

G

G

A

A

R

R

R

S

K

L

K

R

D

E

L

A

Y

F

L

Y

L

T

E

E

L

E

A

Y

E
x
D

Q

Q

W

L

A

Q

R

A

E

E

I

N

P

P

N

N

F

F

K

Q

F
x
W

L
x
H

P
x
L

V

A

L

L

S

S

E

D

P

P

D

Q

A

P

G

E

D

D

A

N

W

W

T

T

G

G

R

L

V

T

G

G

F

F

V

I

H

H

R

N

A

V

V

L

I

F

E

E

-

N

-

Y

-

L

-

K

D

D

L

H

P

P

D

A

L

P

S

E

A

D

Y

C

Q

E

V

F

Y

Y

A

M

C

C

G

G

A

P

P

P

V

M

M

M

binding flavin-adenine dinucleotide: Y40 (vs. gap), R83 (= R148), A84 (≠ S149), Y85 (= Y150), S86 (= S151), N100 (≠ H165), I101 (= I166), R102 (= R167), A104 (vs. gap), S105 (vs. gap), P106 (vs. gap), L112 (≠ M169), P113 (= P170), P114 (≠ G171), G115 (= G172), Q116 (≠ A173), M117 (≠ F174), S118 (≠ T175), A155 (≠ T214)
binding gamma-Valerolactone: F19 (≠ D116), F68 (vs. gap), M70 (vs. gap), I103 (vs. gap), S105 (vs. gap), P108 (vs. gap), D196 (≠ E254), W207 (≠ F265), H208 (≠ L266), L209 (≠ P267)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 278

7c3bB Ferredoxin reductase in carbazole 1,9a-dioxygenase (fad apo form) (see paper)
26% identity, 92% coverage: 18:333/343 of query aligns to 20:305/306 of 7c3bB

query
sites
7c3bB

Q

S

D

D

E

K

P

S

V

L

L

L

S

V

A

S

A

A

L

L

R

A

Q

N

G

G

I

I

G

G

L

L

P

P

Y
|
Y

G

E

C
|
C

K
x
A

N

S

G
|
G

A
x
G

C
|
C

G
|
G

S

V

C
|
C

K

K

G

F

T

E

V

L

S

E

G

G

E

T

I

V

E

Q

Q

S

R

M

A

W

H

P

S

D

S

A

S

P

A

G

L

L

S

S

N

S

D

R

E

D

K

R

T

E

R

K

G

G

-

N

M

R

A

H

L

L

F

A

C
|
C

C

Q

A

C

T

I

A

A

C

L

T

S

D

D

L

L

E

R

V

I

D

K

I

V

R

A

-

V

E

Q

V

D

A

K

G

Y

V

I

G

P

D

A

V

I

Q

P

V

I

K

S

K

K

L

M

P

E

C

A

R

E

V

V

N

V

A

A

I

V

E

R

R

A

K

L

A

T

D

H

D

D

V

L

V

S

L

V

K

K

L

L

Q

R

-

T

-

D

L

V

P

P

A

A

N

N

E

-

R

-

L

-

Q

-

Y

F

L

L

A

P

G

G

Q

Q

Y

F

-

C

-

L

L

I

E

E

F

A

I

E

L

Q

K

L

D

P

G

G

K

V

R

V

R

R

S

A

Y

Y

S

S

M

M

A

A

N

N

A

S

P

M

H

N

T

P

E

D

G

G

P

F

I

W

E

E

L

F

H

Y

I

I

R

K

H

R

M

V

P

P

G

-

A

-

F

F

T

S

D

P

H

W

V

L

F

F

N

E

T

N

M

R

K

K

E

V

R

G

D

A

I

R

L

L

R

F

F

L

E

T

A

G

P

P

L

M

G

G

T

T

F

S

F

F

L

F

R

R

E

P

D

G

S

T

D

G

K

R

P

K

I

S

V

L

L

C

L

I

A

G

S

G

G

G

T

A

G

G

F

-

A

-

P

-

L

L

K

S

A

Y

I

A

V

A

E

A

H

I

A

A

V

R

F

A

K

S

N

I

L

R

N

E

R

T

P

D