SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS16460 FitnessBrowser__BFirm:BPHYT_RS16460 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see paper)
24% identity, 79% coverage: 41:403/457 of query aligns to 56:388/444 of Q8NLB7

query
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Q8NLB7

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D57 (= D42) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
R103 (= R94) mutation to A: Loss of transport activity.
W309 (vs. gap) mutation to V: Loss of transport activity.
D312 (= D321) mutation to A: Loss of transport activity.
R313 (= R322) mutation to A: Loss of transport activity.
I317 (= I326) mutation I->H,Y: Loss of transport activity.
R386 (≠ I401) mutation to A: Loss of transport activity.

Sites not aligning to the query:

54 D→A: Loss of transport activity.; D→E: Retains 50% of its transport activity.

Q9Y7Q9 Probable metabolite transporter C2H8.02 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
22% identity, 39% coverage: 83:258/457 of query aligns to 98:279/583 of Q9Y7Q9

query
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Q9Y7Q9

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S267 (≠ T246) modified: Phosphoserine
S269 (= S248) modified: Phosphoserine

Sites not aligning to the query:

289 modified: Phosphoserine
290 modified: Phosphoserine
292 modified: Phosphoserine
330 modified: Phosphoserine

O23492 Inositol transporter 4; Myo-inositol-proton symporter INT4; Protein INOSITOL TRANSPORTER 4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
26% identity, 53% coverage: 92:332/457 of query aligns to 91:349/582 of O23492

query
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O23492

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Sites not aligning to the query:

559:561 LLE→AAA: No effect on targeting.
559:582 mutation Missing: No effect on targeting.
564:565 FK→AA: No effect on targeting.
570:575 RRREKK→AAAAAA: No effect on targeting.

Query Sequence

>BPHYT_RS16460 FitnessBrowser__BFirm:BPHYT_RS16460
MQTHASPSATASPHSAQAPLSKAMVRRIVFSSSVGNALEWFDFLVYGYFATIIAKEFFPM
KDEWLSTLLAIATFGISFLMRPLGAVVLGIYGDRKGRKAALTLAIALMMVGTFTMAVMPP
YASIGIAAPILILLARLVQGFAVGGEFGSATAFMVEHSASRRGYYASWQFASQGLAAITA
AAFGSLLTAWMPPAQLNDWGWRLPFVFGLLVGPVGYYIRSHLDETPEFLALREAREAREV
AGARSTASEEKDASFASQWVNLLLAVGIVAQSTVGVYVLQLYMPMYAVKQLHMPAAASFG
VVVLNGGLQFLLSPVMGALSDRIGRIRIMLTTSILMGTLIYPMFALLQSHPTIGWLLLLQ
GTAGIFKAAYSGPMPALMSEIFPTQVRSTGLSIGYSIGVTIFGGFAPTIVETFIHLTGDK
LAPSYYVLIAAVLSGLSLAVVAWRMRRVRLMHGVQIA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory