SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS17675 FitnessBrowser__BFirm:BPHYT_RS17675 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

7bgnA Crystal structure of mthisn2-amp complex, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
45% identity, 76% coverage: 7:108/135 of query aligns to 4:104/204 of 7bgnA

query
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7bgnA

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binding adenosine monophosphate: W60 (= W63), K62 (= K65), G63 (= G66), T65 (≠ E68), S66 (= S69), T94 (≠ A98), H96 (= H100)
binding zinc ion: D78 (= D81), D80 (= D83), D82 (= D85), C95 (= C99), C102 (= C106)

Sites not aligning to the query:

binding adenosine monophosphate: 121, 128, 135, 136, 137, 180, 203, 204
binding zinc ion: 154, 174, 177

7bgmA Crystal structure of mthisn2, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
45% identity, 76% coverage: 7:108/135 of query aligns to 6:106/213 of 7bgmA

query
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7bgmA

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binding zinc ion: D80 (= D81), D82 (= D83), D84 (= D85), C97 (= C99), C104 (= C106)

Sites not aligning to the query:

binding zinc ion: 163, 183, 186

6j2lA Crystal structure of bi-functional enzyme (see paper)
48% identity, 68% coverage: 16:107/135 of query aligns to 14:104/200 of 6j2lA

query
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6j2lA

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binding zinc ion: D79 (= D81), D81 (= D83), D83 (= D85), C96 (= C99), C103 (= C106)

Sites not aligning to the query:

binding zinc ion: 152, 171, 174

6j2lB Crystal structure of bi-functional enzyme (see paper)
46% identity, 68% coverage: 16:107/135 of query aligns to 13:97/185 of 6j2lB

query
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6j2lB

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binding zinc ion: D78 (= D81), D80 (= D83), D82 (= D85)

Sites not aligning to the query:

binding magnesium ion: 137, 140, 156, 159

Query Sequence

>BPHYT_RS17675 FitnessBrowser__BFirm:BPHYT_RS17675
MNPSAVNWLDKVKWDANGLVPVIAQEASTNDVLMFAWMNREALAKTIETNRAVYFSRSRQ
RLWFKGEESGHVQHVHEVRLDCDEDVVLLKVEQVSGIACHTGRHSCFFQKFEGSVDDGDW
VAVDPVLKDPEHIYK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory