SitesBLAST
Comparing BPHYT_RS18735 FitnessBrowser__BFirm:BPHYT_RS18735 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5nccA Structure of fatty acid photodecarboxylase in complex with fad and palmitic acid (see paper)
37% identity, 95% coverage: 2:526/552 of query aligns to 23:569/578 of 5nccA
- active site: R347 (≠ Q322), L420 (≠ V382), I421 (≠ C383), S507 (≠ D464), A509 (≠ I466), G552 (= G509), Q553 (≠ N510)
- binding flavin-adenine dinucleotide: G30 (= G9), G32 (= G11), T33 (≠ S12), A34 (≠ G13), L53 (= L32), E54 (= E34), A55 (= A35), F74 (≠ V55), W80 (≠ Y63), A98 (≠ P81), G100 (= G83), G105 (= G88), S106 (= S89), N110 (= N93), A111 (= A94), T112 (≠ M95), L113 (≠ I96), V238 (= V219), A278 (= A253), H282 (≠ N257), L286 (= L261), N508 (≠ T465), Q553 (≠ N510), T554 (= T511), G555 (≠ N512), V558 (≠ T515)
6yrvAAA structure of fap after illumination at 100k (see paper)
37% identity, 95% coverage: 2:526/552 of query aligns to 7:560/573 of 6yrvAAA
- binding carbon dioxide: R375 (≠ H359), N499 (≠ T465)
- binding flavin-adenine dinucleotide: G14 (= G9), G16 (= G11), T17 (≠ S12), A18 (≠ G13), L37 (= L32), E38 (= E34), A39 (= A35), F58 (≠ V55), W64 (≠ Y63), A82 (≠ P81), G89 (= G88), S90 (= S89), N94 (= N93), A95 (= A94), T96 (≠ M95), L97 (≠ I96), M191 (≠ T189), V222 (= V219), C264 (≠ A252), A265 (= A253), G266 (= G254), H269 (≠ N257), N499 (≠ T465), A534 (= A500), Q544 (≠ N510), T545 (= T511), G546 (≠ N512)
- binding heptadecane: V377 (≠ C361), G379 (≠ A363), M380 (≠ I364), G386 (vs. gap), T389 (vs. gap), Y390 (vs. gap), F393 (vs. gap), T408 (≠ S379), Q410 (≠ H381)
8b7sA Crystal structure of the chloramphenicol-inactivating oxidoreductase from novosphingobium sp (see paper)
35% identity, 95% coverage: 2:526/552 of query aligns to 4:450/458 of 8b7sA
- binding flavin-adenine dinucleotide: G11 (= G9), G13 (= G11), S14 (= S12), A15 (≠ G13), E35 (= E34), A36 (= A35), W47 (≠ Y63), P65 (= P81), G67 (= G83), V180 (= V219), A214 (= A253), G215 (= G254), A218 (≠ N257), T270 (≠ S336), Y391 (= Y467), A424 (= A500), I435 (≠ T511), N436 (= N512)
A0A248QE08 Fatty acid photodecarboxylase, chloroplastic; CvFAP; EC 4.1.1.106 from Chlorella variabilis (Green alga) (see paper)
37% identity, 95% coverage: 2:526/552 of query aligns to 83:636/654 of A0A248QE08
- TA 93:94 (≠ SG 12:13) binding
- E114 (= E34) binding
- L162 (≠ G85) binding
- S166 (= S89) binding
- NATL 170:173 (≠ NAMI 93:96) binding
- V298 (= V219) binding
- C432 (≠ A339) binding
- R451 (≠ H359) binding
- Y466 (vs. gap) binding
- Q486 (≠ H381) binding
- G622 (≠ N512) binding
4mjwA Crystal structure of choline oxidase in complex with the reaction product glycine betaine (see paper)
38% identity, 96% coverage: 2:530/552 of query aligns to 13:530/532 of 4mjwA
- active site: I333 (≠ A341), P377 (≠ V382), N378 (≠ C383), V464 (≠ I466), H466 (= H468), V509 (≠ G509), N510 (= N510)
- binding flavin-adenine dinucleotide: G20 (= G9), G22 (= G11), S23 (= S12), E44 (= E34), A45 (= A35), W71 (≠ Y65), R89 (= R82), A90 (≠ G83), G95 (= G88), C96 (≠ S89), H99 (≠ I92), N100 (= N93), S101 (≠ A94), I103 (= I96), R231 (≠ T218), A232 (≠ V219), T269 (≠ A253), G270 (= G254), D273 (≠ N257), Y465 (= Y467), H466 (= H468), A500 (= A500), N510 (= N510), P511 (≠ T511), N512 (= N512), V515 (≠ T515)
3ljpA Crystal structure of choline oxidase v464a mutant (see paper)
38% identity, 96% coverage: 2:530/552 of query aligns to 13:530/530 of 3ljpA
- active site: I333 (≠ A341), P377 (≠ V382), N378 (≠ C383), A464 (≠ I466), H466 (= H468), V509 (≠ G509), N510 (= N510)
- binding dihydroflavine-adenine dinucleotide: G22 (= G11), S23 (= S12), E44 (= E34), A45 (= A35), W71 (≠ Y65), R89 (= R82), A90 (≠ G83), G95 (= G88), C96 (≠ S89), H99 (≠ I92), N100 (= N93), S101 (≠ A94), I103 (= I96), A232 (≠ V219), T269 (≠ A253), D273 (≠ N257), Y465 (= Y467), H466 (= H468), D499 (= D499), A500 (= A500), N510 (= N510), P511 (≠ T511), N512 (= N512), V515 (≠ T515)
2jbvA Crystal structure of choline oxidase reveals insights into the catalytic mechanism (see paper)
38% identity, 95% coverage: 2:526/552 of query aligns to 13:526/527 of 2jbvA
- active site: I333 (≠ A341), P377 (≠ V382), N378 (≠ C383), V464 (≠ I466), H466 (= H468), V509 (≠ G509), N510 (= N510)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[(4aS,10aR)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: G22 (= G11), S23 (= S12), E44 (= E34), A45 (= A35), W71 (≠ Y65), A90 (≠ G83), G95 (= G88), C96 (≠ S89), H99 (≠ I92), N100 (= N93), S101 (≠ A94), I103 (= I96), R231 (≠ T218), A232 (≠ V219), T269 (≠ A253), G270 (= G254), D273 (≠ N257), V464 (≠ I466), Y465 (= Y467), H466 (= H468), D499 (= D499), A500 (= A500), N510 (= N510), P511 (≠ T511), N512 (= N512), V515 (≠ T515)
E4QP00 5-(hydroxymethyl)furfural oxidase; 5-hydroxymethylfurfural oxidase; HMFO; Thiol oxidase; EC 1.1.3.47; EC 1.8.3.- from Methylovorus sp. (strain MP688) (see paper)
35% identity, 95% coverage: 3:526/552 of query aligns to 6:527/531 of E4QP00
- V101 (≠ I92) mutation to H: Abolishes activity.
- M103 (≠ A94) mutation to A: 16-fold reduction in catalytic efficiency on vanillyl alcohol.
- V367 (≠ M372) mutation to K: 1.6-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion.; mutation to R: 1.4-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with F-466.
- W369 (= W374) mutation to A: 7.5-fold reduction in catalytic efficiency on vanillyl alcohol.
- V465 (≠ I466) mutation to A: 18-fold reduction in catalytic efficiency on vanillyl alcohol.
- W466 (≠ Y467) mutation to A: 39-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate.; mutation to F: 3.4-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with R-367.
- H467 (= H468) mutation to A: Abolishes activity.
- N511 (= N510) mutation to A: 53-fold reduction in catalytic efficiency on vanillyl alcohol.
4udqA Crystal structure of 5-hydroxymethylfurfural oxidase (hmfo) in the reduced state
35% identity, 95% coverage: 3:526/552 of query aligns to 2:523/525 of 4udqA
- active site: L331 (≠ R331), F364 (≠ H373), W365 (= W374), V461 (≠ I466), H463 (= H468), A506 (≠ G509), N507 (= N510)
- binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), T11 (≠ S12), A12 (≠ G13), E32 (= E34), A33 (= A35), W64 (≠ Y65), G88 (= G83), G93 (= G88), G94 (≠ S89), N98 (= N93), M99 (≠ A94), V101 (≠ I96), V229 (= V219), T261 (≠ A252), A262 (= A253), W462 (≠ Y467), H463 (= H468), A497 (= A500), N507 (= N510), T508 (= T511), N509 (= N512), T512 (= T515)
8bxlB Patulin synthase from penicillium expansum
32% identity, 94% coverage: 3:523/552 of query aligns to 14:583/590 of 8bxlB
- binding flavin-adenine dinucleotide: G20 (= G9), G22 (= G11), T23 (≠ S12), A24 (≠ G13), E44 (= E34), A45 (= A35), W80 (≠ Y63), G100 (= G83), G105 (= G88), S106 (= S89), R109 (≠ I92), N110 (= N93), Y111 (≠ A94), A113 (≠ I96), L253 (≠ T218), A254 (≠ V219), A288 (= A253), Q292 (≠ N257), F525 (≠ Y467), D559 (= D499), A560 (= A500), H570 (≠ N510), P571 (≠ T511), Q572 (≠ N512), L575 (≠ T515)
5oc1A Crystal structure of aryl-alcohol oxidase from pleurotus eryngii in complex with p-anisic acid (see paper)
30% identity, 95% coverage: 3:528/552 of query aligns to 2:563/565 of 5oc1A
- active site: V339 (≠ P321), N413 (≠ V382), A414 (≠ C383), I499 (= I466), H501 (= H468), A544 (≠ G509), H545 (≠ N510)
- binding 4-methoxybenzoic acid: Y91 (≠ A94), I356 (≠ V338), I390 (vs. gap), F396 (≠ M372), T412 (≠ H381), I499 (= I466), H501 (= H468), H545 (≠ N510)
- binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), N11 (≠ S12), A12 (≠ G13), E32 (= E34), A33 (= A35), W60 (≠ Y63), P78 (= P81), G80 (= G83), G85 (= G88), S86 (= S89), H90 (≠ N93), Y91 (≠ A94), V93 (≠ I96), V230 (= V219), S270 (≠ A252), A271 (= A253), G272 (= G254), F500 (≠ Y467), H545 (≠ N510), T546 (= T511), Q547 (≠ N512), I550 (≠ T515)
3fimB Crystal structure of aryl-alcohol-oxidase from pleurotus eryingii (see paper)
30% identity, 95% coverage: 3:528/552 of query aligns to 2:563/565 of 3fimB
- active site: V339 (≠ P321), N413 (≠ V382), A414 (≠ C383), I499 (= I466), H501 (= H468), A544 (≠ G509), H545 (≠ N510)
- binding flavin-adenine dinucleotide: G8 (= G9), N11 (≠ S12), A12 (≠ G13), E32 (= E34), A33 (= A35), W60 (≠ Y63), P78 (= P81), G80 (= G83), G85 (= G88), S86 (= S89), H90 (≠ N93), Y91 (≠ A94), V93 (≠ I96), V230 (= V219), S270 (≠ A252), A271 (= A253), F500 (≠ Y467), H501 (= H468), H545 (≠ N510), T546 (= T511), Q547 (≠ N512), I550 (≠ T515)
4ha6A Crystal structure of pyridoxine 4-oxidase - pyridoxamine complex (see paper)
33% identity, 94% coverage: 4:520/552 of query aligns to 3:498/508 of 4ha6A
- active site: F360 (≠ V382), G361 (≠ C383), H444 (≠ I466), H446 (= H468), G487 (= G509), P488 (≠ N510)
- binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), S11 (= S12), A12 (≠ G13), E32 (= E34), A33 (= A35), W58 (≠ Y63), R77 (= R82), G78 (= G83), G83 (= G88), S84 (= S89), L87 (≠ I92), H88 (≠ N93), A89 (= A94), M90 (= M95), G91 (≠ I96), V218 (= V219), A251 (= A253), G252 (= G254), E255 (≠ N257), H445 (≠ Y467), A478 (= A500), P488 (≠ N510), I489 (≠ T511), H490 (≠ N512)
- binding 4-(aminomethyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol: A89 (= A94), S314 (≠ G330), H444 (≠ I466), H446 (= H468)
3t37A Crystal structure of pyridoxine 4-oxidase from mesorbium loti
33% identity, 94% coverage: 4:520/552 of query aligns to 3:498/509 of 3t37A
- active site: F360 (≠ V382), G361 (≠ C383), H444 (≠ I466), H446 (= H468), G487 (= G509), P488 (≠ N510)
- binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), S11 (= S12), A12 (≠ G13), E32 (= E34), A33 (= A35), W58 (≠ Y63), R77 (= R82), G78 (= G83), R79 (= R84), G83 (= G88), S84 (= S89), H88 (≠ N93), A89 (= A94), G91 (≠ I96), R217 (≠ T218), V218 (= V219), A251 (= A253), E255 (≠ N257), H445 (≠ Y467), A478 (= A500), P488 (≠ N510), I489 (≠ T511), H490 (≠ N512)
4h7uA Crystal structure of pyranose dehydrogenase from agaricus meleagris, wildtype (see paper)
30% identity, 96% coverage: 1:529/552 of query aligns to 14:575/577 of 4h7uA
- active site: A343 (≠ K301), V426 (≠ C383), Y510 (≠ I466), H512 (= H468), A555 (≠ G509), H556 (≠ N510)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-2,3,4-trihydroxy-5-[(4aR)-4a-hydroxy-7,8-dimethyl-2,4-dioxo-3,4,4a,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]pentyl dihydrogen diphosphate (non-preferred name): G22 (= G9), G24 (= G11), T25 (≠ S12), A26 (≠ G13), E46 (= E34), A47 (= A35), W74 (≠ Y63), G99 (= G88), C100 (≠ S89), H103 (≠ I92), N104 (= N93), G105 (≠ A94), V107 (≠ I96), L242 (≠ T218), V243 (= V219), G282 (≠ A253), G283 (= G254), A286 (≠ N257), H512 (= H468), A546 (= A500), H556 (≠ N510), T557 (= T511), Q558 (≠ N512), V561 (≠ T515)
Q3L245 Pyranose dehydrogenase 1; PDH 1; Pyranose:quinone oxidoreductase 1; EC 1.1.99.29 from Leucoagaricus meleagris (Western flat-topped agaric) (Agaricus meleagris) (see 2 papers)
30% identity, 96% coverage: 1:529/552 of query aligns to 39:600/602 of Q3L245
- N100 (≠ G64) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H128 (≠ I92) modified: Tele-8alpha-FAD histidine
- N344 (= N290) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H537 (= H468) active site, Proton acceptor
- H581 (≠ N510) active site
Sites not aligning to the query:
3q9tA Crystal structure analysis of formate oxidase (see paper)
26% identity, 96% coverage: 2:531/552 of query aligns to 6:574/577 of 3q9tA
- active site: A335 (vs. gap), D422 (≠ C383), A508 (≠ I466), H510 (= H468), C552 (≠ G509), R553 (≠ N510)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl(2R,3S,4S)-5-(8-formyl-7-methyl-2,4-dioxo-3,4-dihydrobenzo[g]pteridin-10(2H)-yl)-2,3,4-trihydroxypentyl dihydrogendiphosphate: G15 (= G11), T16 (≠ S12), E37 (= E34), A38 (= A35), W65 (≠ Y63), T85 (≠ P81), R86 (= R82), G87 (= G83), G92 (= G88), S93 (= S89), N97 (= N93), Y98 (≠ A94), F99 (≠ M95), T100 (≠ I96), S229 (≠ V219), S265 (≠ A252), Q266 (≠ A253), E270 (≠ N257), F509 (≠ Y467), D542 (= D499), A543 (= A500), R553 (≠ N510), I554 (≠ T511), Q555 (≠ N512), V558 (≠ T515)
5zu2A Effect of mutation (r554a) on fad modification in aspergillus oryzae rib40formate oxidase (see paper)
26% identity, 96% coverage: 2:531/552 of query aligns to 6:574/577 of 5zu2A
- binding flavin-adenine dinucleotide: G15 (= G11), T16 (≠ S12), E37 (= E34), A38 (= A35), W65 (≠ Y63), T85 (≠ P81), R86 (= R82), G87 (= G83), G92 (= G88), S93 (= S89), N97 (= N93), Y98 (≠ A94), T100 (≠ I96), S229 (≠ V219), S265 (≠ A252), Q266 (≠ A253), E270 (≠ N257), F509 (≠ Y467), D542 (= D499), A543 (= A500), A553 (≠ N510), I554 (≠ T511), Q555 (≠ N512), V558 (≠ T515)
6xuuA Crystallographic structure of oligosaccharide dehydrogenase from pycnoporus cinnabarinus, glucose-bound form (see paper)
28% identity, 96% coverage: 3:530/552 of query aligns to 15:589/589 of 6xuuA
- binding beta-D-glucopyranose: E35 (≠ D23), N67 (≠ P56), H83 (≠ G74), N104 (≠ P102), D108 (= D106), S139 (vs. gap), A140 (vs. gap), N142 (vs. gap), T166 (= T146), D169 (= D149), T188 (≠ G168), W213 (vs. gap), A225 (= A201), Y226 (= Y202), Q329 (≠ D296), N330 (≠ F297), A331 (≠ T298), Q357 (≠ V324), F414 (≠ H373), K474 (≠ R429), N517 (≠ D464), G520 (vs. gap), A522 (vs. gap), V524 (≠ I466), H526 (= H468), H569 (≠ N510)
- binding flavin-adenine dinucleotide: G21 (= G9), G23 (= G11), L24 (≠ S12), T25 (≠ G13), E45 (= E34), A46 (= A35), F66 (≠ V55), W72 (≠ Y63), H83 (≠ G74), G85 (= G83), G90 (= G88), S91 (= S89), N95 (= N93), G96 (≠ A94), A97 (≠ M95), A98 (≠ I96), M244 (≠ T218), A245 (≠ V219), A286 (= A253), Q290 (≠ N257), A525 (≠ Y467), A559 (= A500), H569 (≠ N510), L570 (≠ T511), S571 (≠ N512), L574 (≠ T515)
- binding alpha-D-glucopyranose: G96 (≠ A94), F414 (≠ H373), W428 (vs. gap)
6xutA Crystallographic structure of oligosaccharide dehydrogenase from pycnoporus cinnabarinus, ligand-free form (see paper)
28% identity, 96% coverage: 3:530/552 of query aligns to 15:589/589 of 6xutA
- binding flavin-adenine dinucleotide: G21 (= G9), G23 (= G11), L24 (≠ S12), T25 (≠ G13), E45 (= E34), A46 (= A35), F66 (≠ V55), W72 (≠ Y63), H83 (≠ G74), G85 (= G83), G90 (= G88), S91 (= S89), N95 (= N93), G96 (≠ A94), A97 (≠ M95), A98 (≠ I96), M244 (≠ T218), A245 (≠ V219), A286 (= A253), G287 (= G254), Q290 (≠ N257), A525 (≠ Y467), H526 (= H468), A559 (= A500), H569 (≠ N510), L570 (≠ T511), S571 (≠ N512), L574 (≠ T515)
Query Sequence
>BPHYT_RS18735 FitnessBrowser__BFirm:BPHYT_RS18735
MQYDYIIVGAGSGGCALASRLADSCPDATIALIEAGPHTDRNLFVNMPVGVAAVVPHKLK
TNYGYLTTPQPGLGGRQGYQPRGRGFGGSSAINAMIYTRGHPLDYDEWAELGCEGWSWTE
VLPYFRRTEGNQRGADAWHGDSGPLTVSDLRYQNPFSRRFVQAAIEAGYKPNSDFNGADQ
EGIGFYQVTQRDGRRCSVARAYIYDRARPNLHTIADATVLRVAFNGKRASGVEIVRGGRT
ETLEARAEVVLAAGAFNSPQLLMCSGIGPAAHLQSLGIAVLHDAPEVGQNLIDHVDFTIN
KRVSSIEPTGFSIRGIARMLPQFVTFMRHGRGMLSSNVAEAGGFLKSKPTLDRPDLQLHF
CAAIVDDHNRHMHWGHGYSLHVCVLRPHSRGTVTLASADARTAPVIDPRFFSDPRDLDLL
VDGARMARRILDAPSLALHGGSELYTHSGQSDAELRRTIVEHADTIYHPVATCRMGGDAR
SVVDPQLRVRGVTGLRIVDASVMPTLIGGNTNSPTVMIGERAADLIAAARRASQTELKIA
STSRAGMAADTA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory