SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS19525 FitnessBrowser__BFirm:BPHYT_RS19525 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

P61459 Pterin-4-alpha-carbinolamine dehydratase; PHS; 4-alpha-hydroxy-tetrahydropterin dehydratase; Dimerization cofactor of hepatocyte nuclear factor 1-alpha; DCoH; Dimerization cofactor of HNF1; Phenylalanine hydroxylase-stimulating protein; Pterin carbinolamine dehydratase; PCD; EC 4.2.1.96 from Rattus norvegicus (Rat) (see 2 papers)
60% identity, 96% coverage: 3:94/96 of query aligns to 6:99/104 of P61459

query
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P61459

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C82 (≠ A77) mutation to R: Reduced dehydratase activity. No impact on hydroxytetrahydrobiopterin-binding.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine

P61457 Pterin-4-alpha-carbinolamine dehydratase; PHS; 4-alpha-hydroxy-tetrahydropterin dehydratase; Dimerization cofactor of hepatocyte nuclear factor 1-alpha; DCoH; Dimerization cofactor of HNF1; Phenylalanine hydroxylase-stimulating protein; Pterin carbinolamine dehydratase; PCD; EC 4.2.1.96 from Homo sapiens (Human) (see 6 papers)
60% identity, 96% coverage: 3:94/96 of query aligns to 6:99/104 of P61457

query
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P61457

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T79 (= T74) to I: in HPABH4D; increased proteolytic degradation; reduced interaction with HNF1B; loss of HNF1B-coactivator activity; dbSNP:rs121913014
C82 (≠ A77) to R: in HPABH4D; increased proteolytic degradation; reduced dehydratase activity; no impact on hydroxytetrahydrobiopterin-binding; reduced interaction with HNF1B; partial impact on HNF1B-coactivator activity; dbSNP:rs104894177
R88 (= R83) to Q: in HPABH4D; when associated in cis with 27-E--T-104 del; uncertain significance; no impact on HNF1B interaction; no impact on HNF1B-coactivator activity; dbSNP:rs115117837
E97 (≠ D92) to K: in HPABH4D; increased proteolytic degradation; loss of HNF1B interaction; loss of HNF1B-coactivator activity; dbSNP:rs397518416

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine
27:104 natural variant: Missing (in HPABH4D; when associated in cis with Q-88; reduced protein levels; loss of HNF1B interaction; loss of HNF1B-coactivator activity; dbSNP:rs727505360)
87:104 natural variant: Missing (in HPABH4D; increased proteolytic degradation; loss of HNF1B interaction; loss of HNF1B-coactivator activity; dbSNP:rs104894172)
98:104 natural variant: Missing (in HPABH4D; increased proteolytic degradation; loss of HNF1B interaction; loss of HNF1B-coactivator activity; dbSNP:rs121913015)

1dcpA Dcoh, a bifunctional protein-binding transcriptional coactivator, complexed with biopterin (see paper)
60% identity, 96% coverage: 3:94/96 of query aligns to 1:94/99 of 1dcpA

query
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1dcpA

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active site: E53 (≠ Q53), H57 (= H57), H58 (= H58), H75 (= H75), E76 (≠ D76), D84 (= D84)
binding 7,8-dihydrobiopterin: D56 (= D56), H57 (= H57), H58 (= H58), T74 (= T74), H75 (= H75)

1f93A Crystal structure of a complex between the dimerization domain of hnf-1 alpha and the coactivator dcoh (see paper)
60% identity, 96% coverage: 3:94/96 of query aligns to 5:98/103 of 1f93A

query
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1f93A

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active site: E57 (≠ Q53), H61 (= H57), H62 (= H58), H79 (= H75), E80 (≠ D76), D88 (= D84)
binding : N43 (= N39), R44 (≠ E40), G47 (= G43), R51 (= R47), L54 (≠ I50)

2v6tB Crystal structure of a complex of pterin-4a-carbinolamine dehydratase from toxoplasma gondii with 7,8-dihydrobiopterin (see paper)
58% identity, 76% coverage: 20:92/96 of query aligns to 21:93/100 of 2v6tB

query
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2v6tB

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active site: D54 (≠ Q53), H58 (= H57), H59 (= H58), H76 (= H75), D77 (= D76), D85 (= D84)
binding 2-amino-6-(1,2-dihydroxy-propyl)-7,8-dihydro-6h-pteridin-4-one: D57 (= D56), H58 (= H57), H59 (= H58), D77 (= D76)

Query Sequence

>BPHYT_RS19525 FitnessBrowser__BFirm:BPHYT_RS19525
MIQKLTSEQRATQLAGLHGWQAVADRDAIQRQFKFADFNEAFGFMTRVAIKAQEMDHHPE
WFNVYNKVEITLSTHDAGGLTERDIKLATFIDSITA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory