SitesBLAST

L86 (= L99) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P179) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (≠ T184) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
32% identity, 44% coverage: 15:250/537 of query aligns to 3:234/371 of 3puyA

query
sites
3puyA

L

V

R

Q

V

L

H

Q

G

N

I

V

G

T

K

K

T

A

Y
x
W

A

G

E

E

P

V

V

V

L

V

A

S

-

K

D

D

V

I

S

N

L

L

D

D

L

I

H

H

A

E

G

G

E

E

V

F

L

V

A

V

L

F

T

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

S

L

K

R

I

M

I

I

G

A

G

G

L

L

V

E

T

T

P

I

T

T

T

S

G

G

S

D

M

L

T

F

L

I

A

G

G

E

A

K

A

R

Y

M

T

N

-

D

-

T

-

P

P

P

A

A

S

E

R

R

K

-

D

-

A

-

E

-

A

-

L

-

G

G

V

V

R

G

M

M

V

V

M

F

Q
|
Q

E

S

L

Y

N

A

L

L

L

Y

P

P

T

H

L

L

S

S

V

V

A

A

E

E

N

N

L

M

F

S

L

F

N

G

R

-

L

L

P

K

Q

L

R

A

G

G

I

A

F

K

G

K

W

E

I

V

D

I

R

N

A

Q

K

R

L

V

R

N

E

Q

D

V

A

A

R

E

H

V

A

L

M

Q

A

L

Q

A

V

H

G

L

L

L

D

D

A
x
R

I

-

D

K

P

P

D

K

T
x
A

L

L

V
x
S

G

G

T

-

L

-

G

-

I

-

G
|
G

H
x
Q

Q

R

Q

Q

M

R

V

V

E

A

I

I

A

G

R

R

N

T

L

L

I

V

G

A

D

E

C

P

R

S

V

V

L

F

I

L

L

L

D

D

E
|
E

P

P

T

L

A

S

M

N

L

L

T

D

A

A

R

A

E

L

V

R

D

V

L

Q

L

M

F

R

E

I

Q

E

V

I

E

S

R

R

L

L

K

H

A

K

R

R

-

L

G

G

V

R

A

T

L

M

V

I

Y

Y

I

V

S

T

H
|
H

R

D

L

Q

E

V

E

E

L

A

K

M

R

T

I

L

A

A

R

D

R

K

A

I

A

V

V

V

L

L

R

D

D

A

G

G

R

R

L

V

V

A

H

Q

V

V

D

G

-

K

-

P

-

L

E

E

M

L

A

Y

N

H

L

Y

T

P

T

A

D

D

R

R

L

F

V

V

T

A

L

G

M

F

V

I

G

G

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y24), S37 (≠ N48), G38 (= G49), C39 (≠ A50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54), Q81 (= Q95), R128 (≠ A143), A132 (≠ T148), S134 (≠ V150), G136 (= G156), Q137 (≠ H157), E158 (= E178), H191 (= H210)
binding magnesium ion: S42 (= S53), Q81 (= Q95)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
32% identity, 44% coverage: 15:250/537 of query aligns to 3:234/371 of 3puxA

query
sites
3puxA

L

V

R

Q

V

L

H

Q

G

N

I

V

G

T

K

K

T

A

Y
x
W

A

G

E

E

P

V

V

V

L

V

A

S

-

K

D

D

V

I

S

N

L

L

D

D

L

I

H

H

A

E

G

G

E

E

V

F

L

V

A

V

L

F

T

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

S

L

K

R

I

M

I

I

G

A

G

G

L

L

V

E

T

T

P

I

T

T

T

S

G

G

S

D

M

L

T

F

L

I

A

G

G

E

A

K

A

R

Y

M

T

N

-

D

-

T

-

P

P

P

A

A

S

E

R

R

K

-

D

-

A

-

E

-

A

-

L

-

G

G

V

V

R

G

M

M

V

V

M

F

Q
|
Q

E

S

L

Y

N

A

L

L

L

Y

P

P

T

H

L

L

S

S

V

V

A

A

E

E

N

N

L

M

F

S

L

F

N

G

R

-

L

L

P

K

Q

L

R

A

G

G

I

A

F

K

G

K

W

E

I

V

D

I

R

N

A

Q

K

R

L

V

R

N

E

Q

D

V

A

A

R

E

H

V

A

L

M

Q

A

L

Q

A

V

H

G

L

L

L

D

D

A
x
R

I

-

D

K

P

P

D

K

T

A

L

L

V
x
S

G

G

T

-

L

-

G

-

I

-

G
|
G

H
x
Q

Q

R

Q

Q

M

R

V

V

E

A

I

I

A

G

R

R

N

T

L

L

I

V

G

A

D

E

C

P

R

S

V

V

L

F

I

L

L

L

D

D

E

E

P

P

T

L

A

S

M

N

L

L

T

D

A

A

R

A

E

L

V

R

D

V

L

Q

L

M

F

R

E

I

Q

E

V

I

E

S

R

R

L

L

K

H

A

K

R

R

-

L

G

G

V

R

A

T

L

M

V

I

Y

Y

I

V

S

T

H
|
H

R

D

L

Q

E

V

E

E

L

A

K

M

R

T

I

L

A

A

R

D

R

K

A

I

A

V

V

V

L

L

R

D

D

A

G

G

R

R

L

V

V

A

H

Q

V

V

D

G

-

K

-

P

-

L

E

E

M

L

A

Y

N

H

L

Y

T

P

T

A

D

D

R

R

L

F

V

V

T

A

L

G

M

F

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ Y24), G38 (= G49), C39 (≠ A50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54), R128 (≠ A143), S134 (≠ V150), Q137 (≠ H157)
binding beryllium trifluoride ion: S37 (≠ N48), G38 (= G49), K41 (= K52), Q81 (= Q95), S134 (≠ V150), G136 (= G156), H191 (= H210)
binding magnesium ion: S42 (= S53), Q81 (= Q95)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
32% identity, 44% coverage: 15:250/537 of query aligns to 3:234/371 of 3puwA

query
sites
3puwA

L

V

R

Q

V

L

H

Q

G

N

I

V

G

T

K

K

T

A

Y
x
W

A

G

E

E

P

V

V

V

L
x
V

A

S

-

K

D

D

V

I

S

N

L

L

D

D

L

I

H

H

A

E

G

G

E

E

V

F

L

V

A

V

L

F

T

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

S

L

K

R

I

M

I

I

G

A

G

G

L

L

V

E

T

T

P

I

T

T

T

S

G

G

S

D

M

L

T

F

L

I

A

G

G

E

A

K

A

R

Y

M

T

N

-

D

-

T

-

P

P

P

A

A

S

E

R

R

K

-

D

-

A

-

E

-

A

-

L

-

G

G

V

V

R

G

M

M

V

V

M

F

Q
|
Q

E

S

L

Y

N

A

L

L

L

Y

P

P

T

H

L

L

S

S

V

V

A

A

E

E

N

N

L

M

F

S

L

F

N

G

R

-

L

L

P

K

Q

L

R

A

G

G

I

A

F

K

G

K

W

E

I

V

D

I

R

N

A

Q

K

R

L

V

R

N

E

Q

D

V

A

A

R

E

H

V

A

L

M

Q

A

L

Q

A

V

H

G

L

L

L

D

D

A
x
R

I

-

D

K

P

P

D

K

T
x
A

L

L

V
x
S

G
|
G

T

-

L

-

G

-

I

-

G
|
G

H
x
Q

Q

R

Q

Q

M

R

V

V

E

A

I

I

A

G

R

R

N

T

L

L

I

V

G

A

D

E

C

P

R

S

V

V

L

F

I

L

L

L

D

D

E
|
E

P

P

T

L

A

S

M

N

L

L

T

D

A

A

R

A

E

L

V

R

D

V

L

Q

L

M

F

R

E

I

Q

E

V

I

E

S

R

R

L

L

K

H

A

K

R

R

-

L

G

G

V

R

A

T

L

M

V

I

Y

Y

I

V

S

T

H
|
H

R

D

L

Q

E

V

E

E

L

A

K

M

R

T

I

L

A

A

R

D

R

K

A

I

A

V

V

V

L

L

R

D

D

A

G

G

R

R

L

V

V

A

H

Q

V

V

D

G

-

K

-

P

-

L

E

E

M

L

A

Y

N

H

L

Y

T

P

T

A

D

D

R

R

L

F

V

V

T

A

L

G

M

F

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ Y24), V17 (≠ L29), G38 (= G49), C39 (≠ A50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54), R128 (≠ A143), A132 (≠ T148), S134 (≠ V150), Q137 (≠ H157)
binding tetrafluoroaluminate ion: S37 (≠ N48), G38 (= G49), K41 (= K52), Q81 (= Q95), S134 (≠ V150), G135 (= G151), G136 (= G156), E158 (= E178), H191 (= H210)
binding magnesium ion: S42 (= S53), Q81 (= Q95)

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
32% identity, 44% coverage: 15:250/537 of query aligns to 3:234/371 of 3puvA

query
sites
3puvA

L

V

R

Q

V

L

H

Q

G

N

I

V

G

T

K

K

T

A

Y
x
W

A

G

E

E

P

V

V

V

L
x
V

A

S

-

K

D

D

V

I

S

N

L

L

D

D

L

I

H

H

A

E

G

G

E

E

V

F

L

V

A

V

L

F

T

V

G

G

E

P

N

S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

S

L

K

R

I

M

I

I

G

A

G

G

L

L

V

E

T

T

P

I

T

T

T

S

G

G

S

D

M

L

T

F

L

I

A

G

G

E

A

K

A

R

Y

M

T

N

-

D

-

T

-

P

P

P

A

A

S

E

R

R

K

-

D

-

A

-

E

-

A

-

L

-

G

G

V

V

R

G

M

M

V

V

M

F

Q
|
Q

E

S

L

Y

N

A

L

L

L

Y

P

P

T

H

L

L

S

S

V

V

A

A

E

E

N

N

L

M

F

S

L

F

N

G

R

-

L

L

P

K

Q

L

R

A

G

G

I

A

F

K

G

K

W

E

I

V

D

I

R

N

A

Q

K

R

L

V

R

N

E

Q

D

V

A

A

R

E

H

V

A

L

M

Q

A

L

Q

A

V

H

G

L

L

L

D

D

A
x
R

I

-

D

K

P

P

D

K

T
x
A

L

L

V
x
S

G

G

T

-

L

-

G

-

I

-

G

G

H
x
Q

Q

R

Q

Q

M

R

V

V

E

A

I

I

A

G

R

R

N

T

L

L

I

V

G

A

D

E

C

P

R

S

V

V

L

F

I

L

L

L

D

D

E

E

P

P

T

L

A

S

M

N

L

L

T

D

A

A

R

A

E

L

V

R

D

V

L

Q

L

M

F

R

E

I

Q

E

V

I

E

S

R

R

L

L

K

H

A

K

R

R

-

L

G

G

V

R

A

T

L

M

V

I

Y

Y

I

V

S

T

H

H

R

D

L

Q

E

V

E

E

L

A

K

M

R

T

I

L

A

A

R

D

R

K

A

I

A

V

V

V

L

L

R

D

D

A

G

G

R

R

L

V

V

A

H

Q

V

V

D

G

-

K

-

P

-

L

E

E

M

L

A

Y

N

H

L

Y

T

P

T

A

D

D

R

R

L

F

V

V

T

A

L

G

M

F

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ Y24), V17 (≠ L29), G38 (= G49), C39 (≠ A50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54), R128 (≠ A143), A132 (≠ T148), S134 (≠ V150), Q137 (≠ H157)
binding magnesium ion: S42 (= S53), Q81 (= Q95)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
32% identity, 44% coverage: 15:250/537 of query aligns to 4:235/371 of P68187

query
sites
P68187

L

V

R

Q

V

L

H

Q

G

N

I

V

G

T

K

K

T

A

Y

W

A

G

E

E

P

V

V

V

L

V

A

S

-

K

D

D

V

I

S

N

L

L

D

D

L

I

H

H

A

E

G

G

E

E

V

F

L

V

A

V

L

F

T

V

G

G

E

P

N

S

G

G

A

C

G

G

K

K

S

S

T

T

L

L

S

L

K

R

I

M

I

I

G

A

G

G

L

L

V

E

T

T

P

I

T

T

T

S

G

G

S

D

M

L

T

F

L

I

A

G

G

E

A

K

A

R

Y

M

T

N

-

D

-

T

-

P

P

P

A

A

S

E

R

R

K

-

D

-

A

-

E

-

A

-

L

-

G

G

V

V

R

G

M

M

V

V

M

F

Q

Q

E

S

L

Y

N
x
A

L

L

L

Y

P

P

T

H

L

L

S

S

V

V

A

A

E

E

N

N

L

M

F

S

L

F

N

G

R

-

L

L

P

K

Q

L

R

A

G

G

I

A

F
x
K

G

K

W

E

I

V

D

I

R

N

A

Q

K

R

L
x
V

R

N

E

Q

D
x
V

A

A

R
x
E

H

V

A

L

M

Q

A

L

Q
x
A

V

H

G

L

L

L

D

D

A

R

I

-

D

K

P

P

D

K

T

A

L

L

V

S

G

G

T

-

L

-

G

-

I

-

G
|
G

H

Q

Q

R

Q

Q

M

R

V

V

E

A

I

I

A

G

R

R

N

T

L

L

I

V

G

A

D

E

C

P

R

S

V

V

L

F

I

L

L

L

D
|
D

E

E

P

P

T

L

A

S

M

N

L

L

T

D

A

A

R

A

E

L

V

R

D

V

L

Q

L

M

F

R

E

I

Q

E

V

I

E

S

R

R

L

L

K

H

A

K

R

R

-

L

G

G

V

R

A

T

L

M

V

I

Y

Y

I

V

S

T

H

H

R

D

L

Q

E

V

E

E

L

A

K

M

R

T

I

L

A

A

R

D

R

K

A

I

A

V

V

V

L

L

R

D

D

A

G

G

R

R

L

V

V

A

H

Q

V

V

D

G

-

K

-

P

-

L

E

E

M

L

A

Y

N

H

L

Y

T

P

T

A

D

D

R
|
R

L

F

V

V

T

A

L

G

M

F

V

I

G

G

A85 (≠ N98) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ F120) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (≠ L128) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ D131) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ R133) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ Q138) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G156) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D177) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (= R243) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
32% identity, 44% coverage: 15:250/537 of query aligns to 1:232/367 of 1q12A

query
sites
1q12A

L

V

R

Q

V

L

H

Q

G

N

I

V

G

T

K

K

T

A

Y
x
W

A

G

E

E

P

V

V

V

L

V

A

S

-

K

D

D

V

I

S

N

L

L

D

D

L

I

H

H

A

E

G

G

E

E

V

F

L

V

A

V

L

F

T

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

S

L

K

R

I

M

I

I

G

A

G

G

L

L

V

E

T

T

P

I

T

T

T

S

G

G

S

D

M

L

T

F

L

I

A

G

G

E

A

K

A

R

Y

M

T

N

-

D

-

T

-

P

P

P

A

A

S

E

R

R

K

-

D

-

A

-

E

-

A

-

L

-

G

G

V

V

R

G

M

M

V

V

M

F

Q

Q

E

S

L

Y

N

A

L

L

L

Y

P

P

T

H

L

L

S

S

V

V

A

A

E

E

N

N

L

M

F

S

L

F

N

G

R

-

L

L

P

K

Q

L

R

A

G

G

I

A

F

K

G

K

W

E

I

V

D

I

R

N

A

Q

K

R

L

V

R

N

E

Q

D

V

A

A

R

E

H

V

A

L

M

Q

A

L

Q

A

V

H

G

L

L

L

D

D

A
x
R

I

-

D

K

P

P

D

K

T
x
A

L

L

V
x
S

G

G

T

-

L

-

G

-

I

-

G
|
G

H
x
Q

Q

R

Q

Q

M

R

V

V

E

A

I

I

A

G

R

R

N

T

L

L

I

V

G

A

D

E

C

P

R

S

V

V

L

F

I

L

L

L

D

D

E

E

P

P

T

L

A

S

M

N

L

L

T

D

A

A

R

A

E

L

V

R

D

V

L

Q

L

M

F

R

E

I

Q

E

V

I

E

S

R

R

L

L

K

H

A

K

R

R

-

L

G

G

V

R

A

T

L

M

V

I

Y

Y

I

V

S

T

H

H

R

D

L

Q

E

V

E

E

L

A

K

M

R

T

I

L

A

A

R

D

R

K

A

I

A

V

V

V

L

L

R

D

D

A

G

G

R

R

L

V

V

A

H

Q

V

V

D

G

-

K

-

P

-

L

E

E

M

L

A

Y

N

H

L

Y

T

P

T

A

D

D

R

R

L

F

V

V

T

A

L

G

M

F

V

I

G

G

binding adenosine-5'-triphosphate: W10 (≠ Y24), S35 (≠ N48), G36 (= G49), C37 (≠ A50), G38 (= G51), K39 (= K52), S40 (= S53), T41 (= T54), R126 (≠ A143), A130 (≠ T148), S132 (≠ V150), G134 (= G156), Q135 (≠ H157)

8wm7D Cryo-em structure of cyanobacterial nitrate/nitrite transporter nrtbcd in complex with signalling protein pii (see paper)
32% identity, 43% coverage: 15:243/537 of query aligns to 5:229/257 of 8wm7D

query
sites
8wm7D

L

L

R

V

V

V

H

E

G

G

I

V

G

S

K

K

T

I

Y
|
Y

A

P

E

T

P

P

-

E

-

G

-

P

-
x
Y

-

T

V
|
V

L

L

A

D

D

G

V

I

S

D

L

L

D

K

L

V

H

R

A

E

G

G

E

E

V

F

L

V

A

C

L

L

T

I

G

G

E

H

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

S

L

K

N

I

M

I

I

G

S

G

G

L

F

V

N

T

T

P

P

T

S

T

E

G

G

S

V

M

V

T

L

L

L

A

Q

G

D

A

K

A

P

Y

I

T

T

-

E

P

P

A

G

S

P

R

D

K

R

D

-

A

-

E

-

A

-

L

-

G

-

V

-

R

M

M

M

V

V

M

F

Q

Q

E

N

L

Y

N

C

L

L

P

P

T

W

L

L

S

N

V

V

A

F

E

E

N

N

L

V

F

Y

L

L

N

A

R

-

L

-

P

-

Q

V

R

D

G

A

I

V

F

F

G

P

W

N

I

K

D

P

R

Q

A

A

K

E

L

K

R

R

E

A

D

I

A

V

R

R

H

E

A

H

M

L

A

A

Q

M

V

V

G

G

L

L

D

T

A

E

I

A

-

A

-

E

-

K

D

K

P

P

D

S

T

Q

L

I

V

S

G

G

T

-

L

-

G

-

I

-

G

G

H

M

Q

K

Q

Q

M

R

V

V

E

A

I

I

A

A

R

R

N

A

L

L

I

S

G

I

D

R

C

P

R

Q

V

V

L

L

I

I

L

L

D

D

E

E

P

P

T

F

A

G

M

A

L

L

T

D

A

A

R

I

E

T

V

K

D

E

L

E

L

L

F

Q

E

E

Q

E

V

L

E

L

R

Q

L

I

K

W

A

S

-

D

R

H

G

Q

V

V

A

T

L

V

V

L

Y

M

I

I

S

T

H

H

R

D

L

I

E

D

E

E

L

A

K

L

R

F

I

L

A

A

R

D

R

R

A

V

V

M

L

M

R

T

D

N

G

G

R

P

L

A

V

A

H

Q

V

I

D

G

E

E

M

I

A

L

N

D

L

I

T

P

T

F

D

D

R

R

binding adenosine-5'-diphosphate: Y14 (= Y24), Y21 (vs. gap), V23 (= V28), S43 (≠ N48), G44 (= G49), C45 (≠ A50), G46 (= G51), K47 (= K52), S48 (= S53), T49 (= T54)

7w78A Heme exporter hrtba in complex with mg-amppnp (see paper)
36% identity, 41% coverage: 11:229/537 of query aligns to 1:214/218 of 7w78A

query
sites
7w78A

A

A

P

P

V

V

L

L

R

S

V

I

H

T

G

N

I

A

G

S

K

V

T

V

Y
|
Y

A

P

E

D

P

G

V

I

L

S

A

T

D

V

V

T

S

A

L

L

D

D

-

S

-

A

-

N

-

V

-

E

L

I

H

F

A

P

G

G

E

E

V

L

L

V

A

A

L

I

T

V

G

G

E

E

N
x
S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

S

L

K

S

I

I

I

A

G

G

G

F

L

L

V

Q

T

E

P

P

T

T

T

S

G

G

S

T

M

V

T

T

L

L

A

H

G

G

A

A

A

E

Y

G

T

L

P

D

A

A

S

T

R

S

K

T

D

R

A

R

E

E

A

H

L

I

G

G

V

-

R

-

M

F

V

V

M

F

Q
|
Q

E

Q

L

P

N

N

L

L

P

G

T

S

L

L

S

T

V

A

A

R

E

E

N

Q

L

L

F

L

L

I

N

T

R

D

L

-

P

H

Q

L

R

R

G

G

I

I

F

K

G

P

W

R

I

K

D

D

R

R

A

A

K

D

L

-

R

-

E

-

D

-

A

-

R

-

H

E

A

L

M

L

A

A

Q

R

V

V

G

G

L

L

D

K

A

G

I

L

D

G

P

-

D

G

T

R

L

R

V

V

G

A

T

Q

L

L

G

S

I

G

G

G

H

Q

Q

R

Q

Q

M

R

V

V

E

N

I

I

A

A

R

R

N

A

L

L

I

M

G

G

D

N

C

P

R

Q

V

L

L

L

I

L

L

A

D

D

E
|
E

P

P

T

T

A

S

M

A

L

L

T

D

A

A

R

R

E

-

V

-

D

-

L

L

L

S

F

K

E

E

Q

I

V

V

E

E

R

L

L

L

-

R

-

D

-

V

-

T

K

K

A

E

R

F

G

A

V

L

A

A

L

T

V

L

Y

M

I

V

S

T

H

H

R

D

L

R

E

S

E

Q

L

L

K

A

R

-

I

Y

A

A

R

D

R

R

A

F

A

V

V

E

L

M

R

A

D

D

G

G

R

K

binding phosphoaminophosphonic acid-adenylate ester: Y14 (= Y24), S43 (≠ N48), G44 (= G49), G46 (= G51), K47 (= K52), S48 (= S53), T49 (= T54), Q88 (= Q95), E163 (= E178)
binding magnesium ion: S48 (= S53), Q88 (= Q95)

7w79A Heme exporter hrtba in complex with mn-amppnp (see paper)
36% identity, 41% coverage: 11:229/537 of query aligns to 1:214/216 of 7w79A

query
sites
7w79A

A

A

P

P

V

V

L

L

R
x
S

V

I

H

T

G

N

I

A

G

S

K

V

T

V

Y
|
Y

A

P

E

D

P

G

V

I

L

S

A

T

D

V

V

T

S

A

L

L

D

D

-

S

-

A

-

N

-

V

-
x
E

L

I

H

F

A

P

G

G

E

E

V

L

L

V

A

A

L

I

T

V

G

G

E

E

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

S

L

K

S

I

I

I

A

G

G

G

F

L

L

V

Q

T

E

P

P

T

T

T

S

G

G

S

T

M

V

T

T

L

L

A

H

G

G

A

A

A

E

Y

G

T

L

P

D

A

A

S

T

R

S

K

T

D

R

A

R

E

E

A

H

L

I

G

G

V

-

R

-

M

F

V

V

M

F

Q
|
Q

E

Q

L

P

N

N

L

L

P

G

T

S

L

L

S

T

V

A

A

R

E

E

N

Q

L

L

F

L

L

I

N

T

R

D

L

-

P

H

Q

L

R

R

G

G

I

I

F

K

G

P

W

R

I

K

D

D

R

R

A

A

K

D

L

-

R

-

E

-

D

-

A

-

R

-

H

E

A

L

M

L

A

A

Q

R

V

V

G

G

L

L

D

K

A

G

I

L

D

G

P

-

D

G

T

R

L

R

V

V

G

A

T

Q

L

L

G

S

I

G

G

G

H

Q

Q

R

Q

Q

M

R

V

V

E

N

I

I

A

A

R

R

N

A

L

L

I

M

G

G

D

N

C

P

R

Q

V

L

L

L

I

L

L

A

D

D

E
|
E

P

P

T

T

A

S

M

A

L

L

T

D

A

A

R

R

E

-

V

-

D

-

L

L

L

S

F

K

E

E

Q

I

V

V

E

E

R

L

L

L

-

R

-

D

-

V

-

T

K

K

A

E

R

F

G

A

V

L

A

A

L

T

V

L

Y

M

I

V

S

T

H
|
H

R

D

L

R

E

S

E

Q

L

L

K

A

R

-

I

Y

A

A

R

D

R

R

A

F

A

V

V

E

L

M

R

A

D

D

G

G

R

K

binding adenosine-5'-triphosphate: Y14 (= Y24), S43 (≠ N48), G44 (= G49), S45 (≠ A50), G46 (= G51), K47 (= K52), S48 (= S53), T49 (= T54)
binding manganese (ii) ion: S6 (≠ R16), E30 (vs. gap), S48 (= S53), Q88 (= Q95), E163 (= E178), H196 (= H210)

8w9mD Cryo-em structure of the cyanobacterial nitrate transporter nrtbcd in complex with atp (see paper)
31% identity, 43% coverage: 15:243/537 of query aligns to 3:227/256 of 8w9mD

query
sites
8w9mD

L

L

R

V

V

V

H

E

G

G

I

V

G

S

K

K

T

I

Y
|
Y

A

P

E

T

P

P

-

E

-

G

-

P

-

Y

-

T

V

V

L

L

A

D

D

G

V

I

S

D

L

L

D

K

L

V

H

R

A

E

G

G

E

E

V

F

L

V

A

C

L

L

T

I

G

G

E
x
H

N
x
S

G
|
G

A

C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

S

L

K

N

I

M

I

I

G

S

G

G

L

F

V

N

T

T

P

P

T

S

T

E

G

G

S

V

M

V

T

L

L

L

A

Q

G

D

A

K

A

P

Y

I

T

T

-

E

P

P

A

G

S

P

R

D

K

R

D

-

A

-

E

-

A

-

L

-

G

-

V

-

R

M

M

M

V

V

M

F

Q
|
Q

E

N

L

Y

N

C

L

L

P

P

T

W

L

L

S

N

V

V

A

F

E

E

N

N

L

V

F

Y

L

L

N

A

R

-

L

-

P

-

Q

V

R

D

G

A

I

V

F

F

G

P

W

N

I

K

D

P

R

Q

A

A

K

E

L

K

R

R

E

A

D

I

A

V

R

R

H

E

A

H

M

L

A

A

Q

M

V

V

G

G

L

L

D

T

A

E

I

A

-

A

-

E

-

K

D

K

P

P

D

S

T
x
Q

L

I

V
x
S

G

G

T

-

L

-

G

-

I

-

G
|
G

H
x
M

Q

K

Q

Q

M

R

V

V

E

A

I

I

A

A

R

R

N

A

L

L

I

S

G

I

D

R

C

P

R

Q

V

V

L

L

I

I

L

L

D

D

E

Q

P

P

T

F

A

G

M

A

L

L

T

D

A

A

R

I

E

T

V

K

D

E

L

E

L

L

F

Q

E

E

Q

E

V

L

E

L

R

Q

L

I

K

W

A

S

-

D

R

H

G

Q

V

V

A

T

L

V

V

L

Y

M

I

I

S

T

H
|
H

R

D

L

I

E

D

E

E

L

A

K

L

R

F

I

L

A

A

R

D

R

R

A

V

V

M

L

M

R

T

D

N

G

G

R

P

L

A

V

A

H

Q

V

I

D

G

E

E

M

I

A

L

N

D

L

I

T

P

T

F

D

D

R

R

binding adenosine-5'-triphosphate: Y12 (= Y24), H40 (≠ E47), S41 (≠ N48), G42 (= G49), G44 (= G51), K45 (= K52), S46 (= S53), T47 (= T54), Q82 (= Q95), Q135 (≠ T148), S137 (≠ V150), G139 (= G156), M140 (≠ H157), H194 (= H210)
binding magnesium ion: S46 (= S53), Q82 (= Q95)

1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
30% identity, 48% coverage: 14:272/537 of query aligns to 9:266/353 of 1oxvD

query
sites
1oxvD

V

V

L

S

R

K

V

V

H
x
F

G

K

I

K

G

G

K

K

T

V

Y

V

A

A

E

-

P

-

V

-

L

L

A

D

D

N

V

V

S

N

L

I

D

N

L

I

H

E

A

N

G

G

E

E

V

R

L

F

A

G

L

I

T

L

G

G

E

P

N
x
S

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

F

S

M

K

R

I

I

G

A

G

G

L

L

V

D

T

V

P

P

T

S

T

T

G

G

S

E

M

L

T

Y

L

F

-

D

-

R

-

L

-

V

-

A

-

S

-

N

A

G

G

K

A

L

A

I

Y

V

T

P

P

P

A

E

S

D

R

R

K

K

D

-

A

-

E

-

A

-

L

-

G

-

V

I

R

G

M

M

V

V

M

F

Q
|
Q

E

T

L

W

N

A

L

L

L

Y

P

P

T

N

L

L

S

T