SitesBLAST
Comparing BPHYT_RS20685 FitnessBrowser__BFirm:BPHYT_RS20685 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P04983 Ribose import ATP-binding protein RbsA; EC 7.5.2.7 from Escherichia coli (strain K12) (see paper)
41% identity, 92% coverage: 14:507/537 of query aligns to 4:491/501 of P04983
- K43 (= K52) mutation to R: Loss of transport.
7mdyC Lolcde nucleotide-bound
32% identity, 41% coverage: 14:231/537 of query aligns to 2:221/226 of 7mdyC
- binding adp orthovanadate: Y12 (= Y24), T19 (vs. gap), V21 (= V28), S40 (≠ E47), S41 (≠ N48), G42 (= G49), S43 (≠ A50), G44 (= G51), K45 (= K52), S46 (= S53), T47 (= T54), Q91 (= Q95), R135 (vs. gap), H138 (≠ I144), E142 (≠ T148), S144 (≠ V150), G145 (= G151), G146 (= G156), E147 (≠ H157), E168 (= E178), N172 (≠ M182), H201 (= H210)
- binding magnesium ion: S46 (= S53), Q91 (= Q95)
P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
32% identity, 41% coverage: 14:231/537 of query aligns to 5:224/233 of P75957
- G42 (= G46) mutation to D: Loss of lipoprotein release when overexpressed.
7arlD Lolcde in complex with lipoprotein and adp (see paper)
32% identity, 41% coverage: 14:231/537 of query aligns to 2:221/222 of 7arlD
7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs
32% identity, 41% coverage: 14:231/537 of query aligns to 4:223/229 of 7v8iD
- binding phosphoaminophosphonic acid-adenylate ester: V23 (= V28), S43 (≠ N48), G44 (= G49), S45 (≠ A50), G46 (= G51), K47 (= K52), S48 (= S53), T49 (= T54), Q93 (= Q95), R137 (vs. gap), H140 (≠ I144), E144 (≠ T148), L145 (= L149), S146 (≠ V150), G147 (= G151), G148 (= G156), E149 (≠ H157), N174 (≠ M182), H203 (= H210)
- binding magnesium ion: S48 (= S53), Q93 (= Q95)
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
32% identity, 44% coverage: 15:250/537 of query aligns to 3:234/374 of 2awnB
2awoA Crystal structure of the adp-mg-bound e. Coli malk (crystallized with adp-mg) (see paper)
32% identity, 44% coverage: 15:250/537 of query aligns to 3:234/372 of 2awoA
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
31% identity, 44% coverage: 15:250/537 of query aligns to 4:235/369 of P19566
- L86 (= L99) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P179) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (≠ T184) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
Sites not aligning to the query:
- 306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
32% identity, 44% coverage: 15:250/537 of query aligns to 3:234/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y24), V17 (≠ L29), P36 (≠ E47), S37 (≠ N48), G38 (= G49), C39 (≠ A50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54), Q81 (= Q95), R128 (≠ A143), A132 (≠ T148), S134 (≠ V150), G135 (= G151), G136 (= G156), Q137 (≠ H157), E158 (= E178), H191 (= H210)
- binding magnesium ion: S42 (= S53), Q81 (= Q95), D157 (= D177)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
32% identity, 44% coverage: 15:250/537 of query aligns to 3:234/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ Y24), V17 (≠ L29), S37 (≠ N48), G38 (= G49), C39 (≠ A50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54), R128 (≠ A143), A132 (≠ T148), L133 (= L149), S134 (≠ V150), Q137 (≠ H157)
- binding beryllium trifluoride ion: S37 (≠ N48), G38 (= G49), K41 (= K52), Q81 (= Q95), S134 (≠ V150), G135 (= G151), G136 (= G156), E158 (= E178), H191 (= H210)
- binding magnesium ion: S42 (= S53), Q81 (= Q95), D157 (= D177)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
32% identity, 44% coverage: 15:250/537 of query aligns to 3:234/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ Y24), V17 (≠ L29), S37 (≠ N48), G38 (= G49), C39 (≠ A50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54), R128 (≠ A143), A132 (≠ T148), S134 (≠ V150), Q137 (≠ H157)
- binding tetrafluoroaluminate ion: S37 (≠ N48), G38 (= G49), K41 (= K52), Q81 (= Q95), S134 (≠ V150), G135 (= G151), G136 (= G156), E158 (= E178), N162 (≠ M182), H191 (= H210)
- binding magnesium ion: S42 (= S53), Q81 (= Q95)
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
32% identity, 44% coverage: 15:250/537 of query aligns to 4:235/371 of P68187
- A85 (≠ N98) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ F120) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (≠ L128) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ D131) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ R133) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ Q138) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G156) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D177) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
- R228 (= R243) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
Sites not aligning to the query:
- 241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 267 W→G: Normal maltose transport but constitutive mal gene expression.
- 278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
32% identity, 44% coverage: 15:250/537 of query aligns to 1:232/367 of 1q12A
- binding adenosine-5'-triphosphate: W10 (≠ Y24), V15 (≠ L29), S35 (≠ N48), G36 (= G49), C37 (≠ A50), G38 (= G51), K39 (= K52), S40 (= S53), T41 (= T54), R126 (≠ A143), K129 (≠ D147), A130 (≠ T148), L131 (= L149), S132 (≠ V150), G133 (= G151), G134 (= G156), Q135 (≠ H157), N160 (≠ M182), H189 (= H210)
7w78A Heme exporter hrtba in complex with mg-amppnp (see paper)
36% identity, 41% coverage: 11:229/537 of query aligns to 1:214/218 of 7w78A
- binding phosphoaminophosphonic acid-adenylate ester: Y14 (= Y24), V21 (≠ D31), A23 (≠ S33), E42 (= E47), S43 (≠ N48), G44 (= G49), S45 (≠ A50), G46 (= G51), K47 (= K52), S48 (= S53), T49 (= T54), Q88 (= Q95), E163 (= E178), H196 (= H210)
- binding magnesium ion: S48 (= S53), Q88 (= Q95)
7w79A Heme exporter hrtba in complex with mn-amppnp (see paper)
36% identity, 41% coverage: 11:229/537 of query aligns to 1:214/216 of 7w79A
- binding adenosine-5'-triphosphate: Y14 (= Y24), V21 (≠ D31), A23 (≠ S33), E42 (= E47), S43 (≠ N48), G44 (= G49), S45 (≠ A50), G46 (= G51), K47 (= K52), S48 (= S53), T49 (= T54), Q88 (= Q95), E163 (= E178), H196 (= H210)
- binding manganese (ii) ion: S6 (≠ R16), E30 (vs. gap), S48 (= S53), Q88 (= Q95), E163 (= E178), H196 (= H210)
1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
30% identity, 48% coverage: 14:272/537 of query aligns to 9:266/353 of 1oxvD
- binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ H18), V18 (≠ T23), A20 (= A25), P39 (≠ E47), S40 (≠ N48), G41 (= G49), A42 (= A50), G43 (= G51), K44 (= K52), T45 (≠ S53), T46 (= T54), Q89 (= Q95), E166 (= E178)
- binding magnesium ion: T45 (≠ S53), Q89 (= Q95)
1oxvA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
30% identity, 48% coverage: 14:272/537 of query aligns to 9:266/353 of 1oxvA