SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS21680 BPHYT_RS21680 amino acid permease-associated protein to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 19 hits to proteins with known functional sites (download)

P46349 Gamma-aminobutyric acid permease; GABA permease; 4-aminobutyrate permease; Gamma-aminobutyrate permease; Proline transporter GabP from Bacillus subtilis (strain 168) (see paper)
51% identity, 96% coverage: 4:458/476 of query aligns to 2:450/469 of P46349

query
sites
P46349

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G33 (= G35) mutation to D: Lack of activity.
G42 (= G44) mutation to S: Lack of activity.
G301 (= G310) mutation to V: Lack of activity.
G338 (= G347) mutation to E: Lack of activity.
F341 (= F350) mutation to S: Lack of activity.
G414 (= G423) mutation to R: Lack of activity.

P15993 Aromatic amino acid transport protein AroP; Aromatic amino acid:H(+) symporter AroP; General aromatic amino acid permease; General aromatic transport system from Escherichia coli (strain K12) (see paper)
37% identity, 97% coverage: 1:461/476 of query aligns to 2:453/457 of P15993

query
sites
P15993

M

M

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A

Y103 (≠ W108) Key residue for tryptophan transport; mutation to F: Decreases tryptophan transport to less than 50% of wild-type levels and reduces the ability of tryptophan to inhibit phenylalanine transport from 95 to 62%.

P24207 Phenylalanine-specific permease; Phenylalanine:H(+) symporter PheP from Escherichia coli (strain K12) (see 3 papers)
37% identity, 91% coverage: 4:435/476 of query aligns to 13:435/458 of P24207

query
sites
P24207

S

S

T

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D

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T

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V

T

A

V

T

A

L

I

L

F

L

V

N

Y

W

V

I

L

M

I

I

A

C

I

L

S

A

Q

H

L

L

K

R

L

F

R

R

A

A

R

A

I

M

E

R

R

R

D

Q

A

G

P

R

E

E

K

T

L

-

R

Q

V

F

R

K

M

A

W

L

C

L

Y

Y

P

P

Y

F

L

G

T

N

W

Y

V

L

A

C

I

I

I

A

G

F

M

L

V

G

G

M

I

I

L

L

V

L

A

L

M

M

A

C

F

T

I

M

P

D

E

D

Q

M

R

R

R26 (= R17) mutation R->G,S,Q: Strong decrease in phenylalanine transport activity.
P54 (= P45) mutation to A: 50% of wild-type phenylalanine transport activity.; mutation to G: No change in phenylalanine transport activity.; mutation to L: 26% of wild-type phenylalanine transport activity.
F87 (= F78) mutation to L: No effect on phenylalanine transport activity.
F90 (≠ Y81) mutation to L: 65% of wild-type phenylalanine transport activity.
Y92 (≠ K89) mutation to L: 41% of wild-type phenylalanine transport activity.
Y94 (≠ A91) mutation to L: 69% of wild-type phenylalanine transport activity.
W95 (≠ S92) mutation to L: 10% of wild-type phenylalanine transport activity.
F98 (≠ L95) mutation to L: No effect on phenylalanine transport activity.
F101 (= F98) mutation to L: 38% of wild-type phenylalanine transport activity.
W105 (= W102) mutation to L: 39% of wild-type phenylalanine transport activity.
Y107 (= Y104) mutation to L: No effect on phenylalanine transport activity.
W108 (= W105) mutation to L: 71% of wild-type phenylalanine transport activity.
F111 (≠ W108) mutation to L: 60% of wild-type phenylalanine transport activity.; mutation to Y: Enables the transport of tryptophan to almost the same steady-state level as that of phenylalanine.
E118 (= E115) mutation E->G,L,V,N: Loss of activity.
K168 (= K165) mutation K->L,R: Strong decrease in phenylalanine transport activity.; mutation to N: Loss of activity.
E226 (= E226) mutation E->A,Q,K,R,W: Loss of activity.
R252 (= R252) mutation R->D,E,F,W,P: Loss of activity.
P341 (= P340) mutation to A: 5% of wild-type phenylalanine transport activity.; mutation P->G,Q,K,R: Loss of activity.; mutation to S: 3% of wild-type phenylalanine transport activity.; mutation to T: 17% of wild-type phenylalanine transport activity.

Sites not aligning to the query:

442 P→A: 46% of wild-type phenylalanine transport activity.; P→G: 52% of wild-type phenylalanine transport activity.; P→L: 43% of wild-type phenylalanine transport activity.

P25737 Lysine-specific permease LysP; Lysine transporter LysP; Trigger transporter LysP from Escherichia coli (strain K12) (see 2 papers)
38% identity, 82% coverage: 1:392/476 of query aligns to 1:397/489 of P25737

query
sites
P25737

M
|
M

Q

V

R

S

S

E

T

T

D

K

T

T

E

E

-

A

-

P

-

G

L

L

K

R

A

R

G

E

L

L

K

K

Q

A

R

R

H

H

M

L

T

T

M

M

I

I

A

A

L

I

G

G

V

S

I

I

G

G

A

T

G

G

L

L

F

F

V

V

G

A

S

S

G

G

V

A

V

T

V

I

Q

S

Q

Q

A

A

G

G

P

P

A

G

-

G

A

A

V

L

L

L

S

S

F

Y

L

M

I

L

T

I

G

G

A

L

L

M

V

V

V

Y

L

F

V

L

M

M

R

T

M

S

L

L

G

G

E

E

M

L

A

A

C

A

A

Y

M

M

P

P

A

V

V

S

G

G

S

S

F

F

Y

A

E

T

Y

Y

A

-

R

-

L

-

A

-

F

-

G

-

G

G

K

Q

R

N

A

Y

S

V

G

E

N

E

L

G

A

F

G

G

F

F

L

A

T

L

G

G

W

W

M

N

Y
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Y

W

W

Y

Y

F

N

W
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W

V

A

I

V

V

T

V

I

A

A

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V

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D

A

L

V

V

A

A

G

A

A

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K

L

L

V

V

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Q

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F

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W

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L

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P

P

D

D

V

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P

P

A

G

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W

A

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W

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S

L

A

V

L

L

F

L

L

V

G

T

V

L

I

T

F

A

L

T

L

N

N

L

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V

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V

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S

G

Y

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G

G

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E

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E

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W

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F

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K

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V

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M

I

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V

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F

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I

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I

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G

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P

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G

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N

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T

T

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I

L

-

S

-

H

-

G

-

G

G

L

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M

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G

A

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G

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G

-

F

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G

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V

A

A

M

A

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G

G

F
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F

Y

S

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F

-

Q

G

G

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E
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E

I

L

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I

I

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E
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E

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L

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-
x
D

-

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K

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x
D

-

I

-

S

A

V

T

S

P

P

Y

F

V

T

S

L

A

V

L

F

D

Q

A

H

M

A

G

G

I

L

P

L

A

S

A

A

S

A

V

V

M

M

N

N

A

A

I

V

V

I

L

L

T

T

A

A

V

V

L

L

S

S

A

A

L

G

N

N

S

S

G

G

L

M

Y

Y

A

A

A

S

S

T

R

R

M

M

I

L

F

Y

A

T

L

L

T

A

R

C

H

D

G

G

D

K

A

A

P

P

A

R

A

I

L

F

A

A

K

K

V

L

N

S

R

R

R

G

G

G

V

V

P

P

V

R

R

N

A

A

I

L

L

Y

I

A

G

T

T

T

V

V

F

I

G

A

Y

G

A

L

S

C

V

F

V

L

M

T

S

S

Y

M

V

F

S

G

P

N

D

Q

T

T

V

V

F

Y

A

L

F

W

L

L

V

L

N

N

S

T

Y

S

G

G

T

M

V

T

A

G

I

F

F

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V

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Y

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V

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S

S

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H

L

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R

L

F

R

R

M1 (= M1) modified: Initiator methionine, Removed
Y102 (= Y104) mutation to L: Retains 4% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
W106 (= W108) mutation to L: Retains 20% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
K163 (= K165) mutation to A: Retains 24% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
F216 (= F221) mutation to L: Retains 13% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
E222 (= E226) mutation to A: Abolishes lysine uptake. Strongly inhibits CadC.
E230 (= E234) mutation to V: Abolishes lysine uptake. Shows significant less inhibition of CadC.
D275 (vs. gap) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-278.
D278 (vs. gap) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-275.

Sites not aligning to the query:

438 E→A: Retains 14% of wild-type lysine uptake activity. Is unable to inhibit CadC.
443 D→A: Retains 11% of wild-type lysine uptake activity. Is unable to inhibit CadC.
446 D→A: Retains 13% of wild-type lysine uptake activity. Is unable to inhibit CadC.

P04817 Arginine permease CAN1; Canavanine resistance protein 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
35% identity, 89% coverage: 6:430/476 of query aligns to 80:515/590 of P04817

query
sites
P04817

D

Q

T

N

T

A

E

E

L

V

K

K

A

R

G

E

L

L

K

K

Q

Q

R

R

H

H

M

I

T

G

M

M

I

I

A

A

L

L

G

G

V

T

I

I

G

G

A

T

G

G

L

L

F

F

V

I

G

G

S

L

G

S

V

T

V
x
P

V

L

Q

T

Q

N

A

A

G

G

P

P

-

V

A

G

A

A

V

L

L

I

S

S

F

Y

L

L

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F

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M

G

G

A

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L

L

G

G

E

E

M

M

A

A

C

T

A

F

M

I

P
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P

A
x
V

V

T

G

S

S
|
S

F

F

Y

T

E

V

Y

F

A

S

R

Q

L

R

A

F

F

L

G

S

G

P

K

A

R

F

A

G

S

A

G

A

N

N

L

-

A

-

G

-

F

-

L

-

T

-

G

G

W

Y

M

M

Y
|
Y

W

W

Y

F

F

S

W

W

V

A

I

I

V

T

V

F

A

A

V

L

E

E

A

L

V

S

A

V

G

V

A

G

K

Q

L

V

V

I

Q

Q

F

F

W

W

L

T

P

Y

D

K

V

V

P

P

A

L

W

A

A

A

I

W

S

I

L

S

V

I

L

F

L

W

V

V

T

I

L

I

T

T

A

I

T

M

N

N

L

L

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S

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V

V

G

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S

Y

Y

Y

G

G

E

E

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F

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S

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A

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C

G

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C

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M

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C

G

G

-

A

-

G

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-

V

-

G

-

F

-

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A

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K

R

H

N

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G

A

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D

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x
G

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P

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K

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N

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D

P

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Q

-

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x
T

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x
S

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x
Y

-

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T

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G

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x
W

L

L

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L

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N

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I

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A

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x
F

F

F

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A

Y

W

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L

L

F

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I

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S

Q

H

L

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R

-

F

-

M

-

Q

-

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L

L

R

K

A

Y

R

R

-

G

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R

R

D

D

A

-

P

-

E

-

K

E

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V

F

R

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C

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P

P

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F

F

P113 (≠ V39) mutation to L: In CAN1-343; confers citrulline transport activity in GAP1-deleted cells.
P148 (= P73) mutation to L: In CAN1-337; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, but not sensitivity to L-aspartic acid alpha-hydroxamate or p-fluoro-L-phenylalanine.
V149 (≠ A74) mutation to F: In CAN1-315; confers citrulline transport activity in GAP1-deleted cells.
S152 (= S77) mutation to F: In CAN1-342; confers citrulline transport activity in GAP1-deleted cells.
Y173 (= Y104) mutation to D: In CAN1-306; confers citrulline transport activity in GAP1-deleted cells.; mutation to H: In CAN1-327; confers citrulline transport activity in GAP1-deleted cells.
G308 (≠ A233) mutation to A: In CAN1-341; confers citrulline transport activity in GAP1-deleted cells.
P313 (= P238) mutation to S: In CAN1-329; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, L-aspartic acid alpha-hydroxamate and p-fluoro-L-phenylalanine.
TS 354:355 (vs. gap) mutation Missing: In CAN1-318; confers citrulline transport activity in GAP1-deleted cells.
Y356 (vs. gap) mutation to H: In CAN1-340; confers citrulline transport activity in GAP1-deleted cells.; mutation to N: In CAN1-339; confers citrulline transport activity in GAP1-deleted cells.
W451 (≠ F368) mutation to C: In CAN1-328; confers citrulline transport activity in GAP1-deleted cells.; mutation to L: In CAN1-316; confers citrulline transport activity in GAP1-deleted cells.; mutation to S: In CAN1-335; confers citrulline transport activity in GAP1-deleted cells.
F461 (≠ I378) mutation to S: In CAN1-307; confers citrulline transport activity in GAP1-deleted cells.

P19145 General amino-acid permease GAP1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
32% identity, 82% coverage: 5:392/476 of query aligns to 81:475/602 of P19145

query
sites
P19145

T

T

D

A

T

Q

T

T

E

P

L

L

K

K

A

H

G

H

L

L

K

K

Q

N

R

R

H

H

M

L

T

Q

M

M

I

I

A

A

L

I

G

G

V

A

I

I

G

G

A

T

G

G

L

L

F

L

V

V

G

G

S

S

G

G

V

T

V

A

V

L

Q

R

Q

T

A

G

G

G

P

P

A

A

A

S

V

L

L

L

-

I

S

G

F

W

L

G

I

S

T

T

G

G

A

T

L

M

V

I

V

Y

L

A

V

M

M

V

R

M

M

A

L

L

G

G

E

E

M

L

A

A

C

V

A

I

M

F

P

P

A

I

V

S

G

G

S

G

F

F

Y

T

E

T

Y

Y

A

A

R

-

L

-

A

-

F

-

G

-

K

T

R

R

A

F

S

I

G

D

N

E

L

S

A

F

G

G

F

Y

L

A

T

N

G

N

W

F

M

N

Y

Y

W

M

Y

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W

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V

L

A

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V

L

E

E

A

I

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G

A

A

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F

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W

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A

R

I

D

S

G

L

F

V

V

L

A

L

L

-

F

-

W

V

L

T

A

L

I

T

V

A

I

T

I

N

N

L

M

V

F

S

G

V

V

G

K

S

G

Y

Y

G

G

E

E

F

A

E

E

F

F

W

V

F

F

A

S

S

F

I

I

K

K

V

V

A

I

A

T

I

V

M

V

V

G

F

F

L

I

F

I

L

L

G

G

G

-

M

-

Y

I

V

I

L

L

G

N

L

C

W

G

P

G

A

G

A

P

K

T

H

G

V

G

T

Y

A

I

V

G

L

G

P

K

T

Y

L

W

L

H

S

D

H

P

G

G

G

A

L

F

M

A

P

G

K

D

G

T

I

P

G

G

P

A

V

K

L

F

S

K

G

G

A

V

-

C

-

S

-

V

-

F

V

V

A

T

A

A

T

A

G

F

F

S

Y

F

F
x
A

G

G

A

S

E

E

I

L

V

V

T

G

I

L

A

A

A

S

E

E

A

S

Q

V

E

E

P

P

A

R

K

K

A

S

V

V

A

P

K

K

A

A

T

A

N

K

S

Q

V

V

I

F

T

W

R

R

V

I

L

T

V

L

F

F

Y

Y

V

I

G

L

S

S

I

L

L

L

L

M

V

I

V

G

A

L

L

L

V

V

P

P

W

Y

N

N

S

D

P

K

K

S

M

L

-

I

-

G

-

A

-

S

-

V

-

D

-

A

A

A

T

S

P

P

Y

F

V

V

S

I

A

A

L

I

D

K

A

T

M

H

G

G

I

I

P

K

A

G

A

L

A

P

S

S

V

V

M

V

N

N

A

V

I

V

V

I

L

L

T

I

A

A

V

V

L

L

S

S

A

V

L

G

N

N

S

S

G

A

L

I

Y

Y

A

A

A

C

S

S

R

R

M

T

I

M

F

V

A

A

L

L

T

A

R

E

H

Q

G

R

D

F

A

L

P

P

A

E

A

I

L

F

A

S

K

Y

V

V

N

D

R

R

R

K

G

G

V

R

P

P

V

L

R

V

A

G

I

I

L

A

I

V

G

T

T

S

V

A

F

F

G

G

Y

L

A

I

S

A

V

F

V

V

M

A

S

A

Y

S

V

K

S

K

P

E

D

G

T

E

V

V

F

F

A

N

F

W

L

L

V

L

N

A

S

L

Y

S

G

G

T

L

V

S

A

S

I

L

F

F

V

T

Y

W

V

G

L

G

I

I

A

C

I

I

S

C

Q

H

L

I

K

R

L

F

R

R

A297 (≠ F223) mutation to V: Impairs basic amino-acids transport and regulation by these amino-acids.

Sites not aligning to the query:

76 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q9URZ4 Cationic amino acid transporter 1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
30% identity, 89% coverage: 6:429/476 of query aligns to 76:503/587 of Q9URZ4

query
sites
Q9URZ4

D

D

T

G

T

T

E

A

L

L

K

K

A

R

G

S

L

L

K

K

Q

S

R

R

H

H

M

M

T

Q

M

M

I

I

A

S

L

I

G

G

V

A

I

I

G

G

A

T

G

G

L

L

F

Y

V

V

G

G

S

S

G

G

V

S

V

L

Q

A

Q

D

A

G

G

G

P

P

A

A

A

S

V

V

-

I

L

I

S

N

F

Y

L

S

I

L

T

I

G

G

A

I

L

M

V

M

V

F

L

F

V

I

M

V

R

Y

M

A

L

L

G

G

E

E

M

M

A

A

C

V

A

A

M

Y

P

P

A

V

V

A

G

G

S

G

F

F

Y

N

E

T

Y

Y

A

A

R

-

L

-

A

-

F

-

G

-

K

T

R

R

A

F

S

I

G

D

N

P

L

A

A

W

G

G

F

F

L

A

T

V

G

S

W

W

M

N

Y

Y

W

F

Y

I

F

N

W

Y

V

F

I

V

V

T

V

F

A

P

V

L

E

E

A

L

V

T

A

T

G

C

A

A

K

I

L

T

V

F

Q

R

F

Y

W

W

L

T

P

-

D

D

V

I

P

N

A

S

W

A

A

A

I

W

S

I

L

S

V

I

L

F

L

L

V

V

T

V

L

I

T

I

A

I

T

V

N

N

L

L

V

F

S

G

V

V

G

R

S

A

Y

Y

G

G

E

E

F

V

E

E

F

F

W

I

F

L

A

S

S

T

I

V

K

K

V

V

A

V

A

A

I

T

M

F

V

G

F

F

L

I

F

I

L

L

G

A

G

I

M

I

Y

I

V

N

L

C

G

G

L

G

W

V

P

P

A

-

A

T

K

D

H

H

V

R

T

G

A

Y

V

I

L

G

P

G

T

S

L

I

S

K

H

H

G

K

G

P

L

F

M

R

P

H

-

G

-

F

K

K

G

G

I

F

G

C

P

S

V

V

L

F

S

T

G

T

A

A

V

A

A

F

A

S

T

-

G

-

F

-

Y

F

F

S

G

G

A

T

E

E

I

V

V

I

T

G

I

L

A

A

E

E

A

V

Q

D

E

N

P

P

A

Q

K

K

A

A

V

L

A

P

K

H

A

A

T

V

N

K

S

Q

V

V

I

F

T

W

R

R

V

I

L

A

V

I

F

F

Y

Y

V

V

G

V

S

S

I

L

L

I

L

L

V

I

V

G

A

L

L

L

V

I

P

S

W

P

N

D

S

D

P

P

K

N

M

L

-

M

-

G

-

N

-

G

-

S

-

T

-

S

A

V

T

S

P

P

Y

F

V

V

S

L

A

A

L

I

D

K

A

E

M

A

G

N

I

I

P

K

A

G

A

L

A

P

S

S

V

V

M

F

N

N

A

A

I

V

V

I

L

I

T

I

A

S

V

V

L

V

S

S

A

V

L

T

N

N

S

S

G

S

L

T

Y

Y

A

T

A

A

S

G

R

R

M

T

I

L

F

H

A

G

L

M

T

A

R

N

H

L

G

K

D

Q

A

A

P

P

A

S

A

F

L

F

A

K

K

Y

V

T

N

D

R

R

R

L

G

G

V

R

P

P

V

L

R

L

A

A

I

M

L

I

I

V

G

V

T

L

V

L

F

F

G

G

Y

F

A

F

S

A

V

Y

V

I

M

N

S

E

Y

A

V

D

S

K

-

N

-

G

-

N

-

D

-

V

P

S

D

D

T

T

V

V

F

F

A

N

F

W

L

L

V

L

N

A

S

L

Y

S

G

G

T

L

V

S

A

N

I

F

F

F

V

T

Y

W

V

G

L

S

I

I

A

C

I

L

S

C

Q

H

L

I

K

I

L

F

R

R

A

L

R

A

I

F

E

K

R

K

D

Q

A

G

P

-

E

H

K

S

L

L

R

K

V

E

R

L

M

G

W

F

C

V

Y

S

P

P

Y

M

L

G

T

I

W

W

V

G

A

S

I

C

I

I

G

G

M

L

-

F

V

F

G

N

I

I

L

L

V

C

A

L

M

M

A

A

Sites not aligning to the query:

29 modified: Phosphoserine
30 modified: Phosphoserine
37 modified: Phosphoserine

P48813 High-affinity glutamine permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
27% identity, 92% coverage: 1:439/476 of query aligns to 136:601/663 of P48813

query
sites
P48813

M

Q

Q

E

R

Q

S

E

T

Q

D

K

T

Q

T

E

E

N

L

L

K

K

A

K

G

S

L

I

K

K

Q

P

R

R

H

H

M

T

T

V

M

M

I

M

A

S

L

L

G

G

G

T

V

G

I

I

G

G

A

T

G

G

L

L

F

L

V

V

G

G

S

N

G

S

V

K

V

V

V

L

Q

N

A

A

G

G

P

P

A

G

-

L

V

I

L

I

S

G

F

Y

L

A

I

I

T

M

G

G

A

S

L

C

V

V

V

Y

L

C

V

I

M

I

R

Q

M

A

L

C

G

G

E

E

M

L

A

A

C

V

A

I

M

Y

P

S

A

D

-

L

V

I

G

G

S

G

F

F

Y

N

E

T

Y

Y

A

P

R

L

L

F

A

L

F

V

G

D

G

P

K

A

R

-

A

-

S

-

G

-

N

-

L

-

A

L

G

G

F

F

L

S

T

V

G

A

W

W

M

L

Y

F

W

C

Y

L

F

Q

W

W

V

L

I

C

V

V

V

C

A

P

V

L

E

E

A

L

V

V

A

T

G

A

A

S

K

M

L

T

V

I

Q

K

F

Y

W

W

L

T

P

T

D

S

V

V

P

N

A

P

W

D

A

V

I

F

S

V

L

V

V

I

L

F

L

Y

V

V

T

L

L

I

T

V

A

V

T

I

N

N

L

V

V

F

S

G

V

A

G

K

S

G

Y

Y

G

A

E

E

F

A

E

D

F

F

W

F

F

F

A

N

S

C

I

C

K

K

V

I

A

L

A

M

I

I

M

V

V

G

F

F

L

F

F

I

L

L

G

A

G

I

M

I

Y

I

V

D

L

C

G

G

L

G

W

A

P

G

A

T

A

D

K

G

H

Y

V

I

T

G

A

S

V

K

L

Y

-

W

-

R

-

D

P

P

T

G

L

A

L

F

S

R

H

G

G

D

G

T

L

P

M

I

P

Q

K

R

G

F

I

K

G

G

P

V

V

V

L

A

S

T

G

F

A

V

V

T

A

A

T

-

G

-

F

F

Y

A

F

F

G

G

-

M

A

S

E

E

I

Q

V

L

T

A

I

M

A

T

A

A

A

S

E

E

A

Q

Q

S

E

N

P

P

A

R

K

K

A

A

V

I

A

P

K

S

A

A

T

A

N

K

S

K

V

M

I

I

T

Y

R

R

V

I

L

L

V

F

F

V

Y

F

V

L

G

A

S

S

I

L

L

T

L

L

V

V

V

G

A

F

L

L

V

V

P

P

W

Y

N

T

S

S

P

D

K

Q

M

L

-

L

-

G

-

A

-

G

-

S

-

A

-

T

-

K

A

A

T

S

P

P

Y

Y

V

V

S

I

A

A

L

V

D

S

A

S

M

H

G

G

I

V

P

R

A

V

A

P

S

H

V

F

M

I

N

N

A

A

I

V

V

I

L

L

T

L

A

S

V

V

L

L

S

S

A

V

L

A

N

N

S

G

G

A

L

F

Y

Y

A

T

A

S

S

S

R

R

M

I

I

L

F

M

A

S

L

L

T

A

R

K

H

Q

G

G

D

N

A

A

P

P

A

K

A

C

L

F

A

D

K

Y

V

I

N

D

R

R

R

E

G

G

V

R

P

P

V

A

R

A

A

A

I

M

L

L

I

V

G

S

T

A

V

L

F

F

G

G

Y

V

A

I

S

A

V

F

V

C

M

A

S

S

Y

S

V

K

S

K

P

E

D

E

T

D

V

V

F

F

A

T

F

W

L

L

V

L

N

A

S

I

Y

S

G

G

T

L

V

S

A

Q

I

L

F

F

V

T

Y

W

V

I

L

T

I

I

A

C

I

L

S

S

Q

H

L

I

K

R

L

F

R

R

A

R

R

A

I

M

E

K

-

V

-

Q

-

G

R

R

D

S

A

L

P

G

E

E

-

V

-

G

-

Y

K

K

L

S

R

Q

V

V

R

G

M

V

W

W

C

G

Y

S

P

A

Y

Y

L

A

T

V

-

L

-

M

-

V

-

L

-

A

-

L

-

I

-

A

-

Q

-

F

W

W

V

V

A

A

I

I

-

A

-

P

I

I

G

G

M

G

V

G

G

G

I

K

L

L

V

S

A

A

M

Q

A

S

F

F

I

F

P

E

E

N

Q

Y

-

L

R

A

Q

M

P

P

L

I

W

W

Sites not aligning to the query:

132 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q03770 SPS-sensor component SSY1; Amino-acid permease homolog SSY1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
23% identity, 90% coverage: 3:431/476 of query aligns to 270:770/852 of Q03770

query
sites
Q03770

R

R

S

A

T

T

D

R

T

K

T

Y

E

H

L

I

K

Q

A

R

G

K

L

L

K

K

Q

V

R

R

H

H

M

I

T

Q

M

M

I

L

A

S

L

I

G

G

G

A

V

C

I

F

G

S

A

V

G

G

L

L

F

F

V

L

G

T

S

S

G

G

V

K

V

A

V

F

Q

S

Q

I

A

A

G

G

P

P

-

F

A

G

A

T

V

L

L

L

S

G

F

F

L

G

I

L

T

T

G

G

A

S

L

I

V

I

V

L

L

A

V

T

M

M

R

L

M

S

L

F

G

T

E

E

M

L

A

S

C

T

A

L

M

I

P

P

A

V

V

S

G

S

S

G

F

F

Y

S

E

G

Y

L

A

A

R

-

L

-

A

-

F

-

G

-

K

S

R

R

A

F

S

V

G

E

N

D

L

A

A

F

G

G

F

F

L

A

T

L

G

G

W

W

M

T

Y

Y

W

W

Y

I

F

S

W

C

V

M

I

L

V

A

V

L

A

P

V

A

E

Q

A

V

V

S

A

S

G

S

A
x
T

K

F

L

Y

V

L

Q

S

F

Y

W

Y

L

N

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A

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N

L

L

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K

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T382 (≠ A120) mutation T->H,L: Constitutively active, up-regulates amino acid permease transcription in response to subthreshold concentrations of amino acids.; mutation to K: In SSY1-102; constitutively active, up-regulates amino acid permease transcription in the absence of amino-acids.; mutation to R: Constitutively active, up-regulates amino acid permease transcription in the absence of amino acids.

6f34A Crystal structure of a bacterial cationic amino acid transporter (cat) homologue bound to arginine. (see paper)
24% identity, 90% coverage: 9:436/476 of query aligns to 22:454/458 of 6f34A

query
sites
6f34A

E

S

L

L

K

R

A

K

G

E

L

L

K
x
G

Q

A

R
x
F

H
x
D

M

L

T

T

M
|
M

I

L

A

G

L

I

G

G

G

A

V
x
I

I

I

G
|
G

A
x
T

G
|
G

L

I

F

F

V

V

G

L

S

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G

G

V

V

V

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-

A

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A

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G

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P

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L

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V

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F

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I

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L

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G

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Y

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K

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G

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W

M

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x
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|
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|
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W

W

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W

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L

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R

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Q

H

binding arginine: I40 (≠ V27), G42 (= G29), T43 (≠ A30), G44 (= G31), E115 (≠ F107), Y116 (≠ W108), A119 (≠ V111), F228 (≠ G220), A229 (≠ F221), I231 (≠ F223), V314 (≠ A306)
binding cholesterol: W201 (= W184), Y202 (≠ P185)
binding : G28 (≠ K15), F30 (≠ R17), D31 (≠ H18), M34 (= M21), A178 (≠ F157), R179 (≠ E158), A186 (≠ K165), I187 (≠ V166), A190 (≠ I169), L194 (= L173), Q296 (≠ I288), V299 (≠ A291)

5oqtA Crystal structure of a bacterial cationic amino acid transporter (cat) homologue (see paper)
24% identity, 90% coverage: 9:436/476 of query aligns to 20:452/456 of 5oqtA

query
sites
5oqtA

E

S

L

L

K

R

A

K

G
x
E

L

L

K
x
G

Q

A

R
x
F

H
x
D

M

L

T

T

M
|
M

I

L

A

G

L

I

G

G

G

A

V
x
I

I

I

G
|
G

A
x
T

G
|
G

L

I

F

F

V

V

G

L

S

T

G

G

V

V

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A

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A

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A

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G

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|
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-

P

-

V

-

P

-

I

-

L

-

A

-

V

M

L

W

F

C

C

-

G

-

Y

-

L

-

V

-

L

-

Q

Y

L

P

P

Y

A

L

M

T

T

W

W

V

I

A

G

I

F

I

V

G

S

M

W

V

L

G

L

I

I

L

G

V

L

A

V

M

I

A

Y

F

F

I

I

P

Y

E

G

Q

R

R

K

Q

H

binding alanine: I38 (≠ V27), G40 (= G29), T41 (≠ A30), G42 (= G31), F226 (≠ G220), A227 (≠ F221), I229 (≠ F223)
binding : E24 (≠ G13), G26 (≠ K15), F28 (≠ R17), D29 (≠ H18), M32 (= M21), A176 (≠ F157), R177 (≠ E158), A184 (≠ K165), A188 (≠ I169), L192 (= L173), Q294 (≠ I288), V297 (≠ A291)

Q9UHI5 Large neutral amino acids transporter small subunit 2; L-type amino acid transporter 2; hLAT2; Solute carrier family 7 member 8 from Homo sapiens (Human) (see 3 papers)
24% identity, 81% coverage: 22:407/476 of query aligns to 48:424/535 of Q9UHI5

query
sites
Q9UHI5

I

I

A

I

L

V

G

G

G

N

V
x
I

I

I

G

G

A

S

G

G

L

I

F

F

V

V

G

S

S

P

G

K

V

G

V

V

V

L

Q

E

Q

N

A

A

G

G

P

S

A

V

-

G

-

L

A

A

V

L

L

I

S

V

F

W

L

I

I

V

T

T

G

G

A

F

L

I

V

T

V

V

L

V

V

G

M

A

R

L

M

C

L
x
Y

G

A

E

E

M

L

A

G

C

V

A

T

M

I

P

P

A

K

V

S

G

G

S

G

F

D

Y

Y

E

S

Y

Y

A

V

R

K

L

D

A

I

F

F

G

G

K

-

R

-

A

-

S

-

G

-

N

-

L

L

A

A

G

G

F

F

L

L

T

R

G

L

W

W

M

I

Y

A

W

-

Y

-

F

V

W

L

V

V

I

I

V

Y

V

P

A

T

V
x
N

E

Q

A

A

V

V

A

I

G

A

A

L

K

T

L

F

V

S

Q

N

F

Y

W

V

L

L

-

Q

-

P

-

L

-

F

-

P

-

T

-
x
C

-

F

-

P

P

P

D

E

V

S

P

G

A

L

W

R

A

L

I

L

S

A

L

A

V

I

L

C

L

L

V

L

T

L

L

L

T

T

A
x
W

T

V

N

N

L

C

V

S

S

S

V

V

G

R

S

W

Y

A

G

T

E

R

F

V

E

Q

F

D

W

I

F

F

A

T

S

A

I

G

K

K

V

L

A

L

A

A

I

L

M

A

V

L

F

I

L

I

F

I

L

M

G

G

G

I

M

V

Y

Q

V

I

L

C

G

K

-

G

-

E

-

Y

L

F

W

W

P

L

A

E

A

P

K

K

H

N

V

-

T

-

A

-

V

-

L

-

P

-

T

-

L

-

S

A

H

F

G

E

G

N

L

F

M

Q

P

E

K

P

G

D

I

I

G

G

P

L

V

V

L

A

S

L

G

A

A

F

V

L

A

Q

A

G

T

S

G
x
F

F

A

Y

Y

F
x
G

G

G

A

W

E

N

I

F

V

L

T

N

I

Y

A

V

A

T

A

E

E

E

A

L

Q

V

E

D

P

P

A

Y

K

K

A

N

V

L

A

P

K

R

A

A

T

I

N

F

S

I

V

S

I

I

T

P

R

L

V

V

L

T

V

F

F

V

Y

Y

V

V

-

F

G

A

S

N

I

V

L

A

L

Y

V

V

V

T

A

A

L

M

V

S

P

P

W

Q

N

E

-

L

-

A

S

S

P

N

K

A

M
x
V

A

A

T

V

P

T

Y

F

V

G

S

E

A

K

L

L

D

-

A

-

M

L

G

G

I

V

P

-

A

-

A

M

A

A

S

W

V

I

M

M

N

P

A

I

I

S

V

V

L

A

T

L

A

S

V

T

L

F

S

G

A

G

L

V

N

N

S

G

G

S

L

L

Y

F

A

T

A

S

S

S

R

R

M

L

I

F

F

F

A

A

L

G

T

A

R

R

H

E

G

G

D

H

A

L

P

P

A

S

A

V

L

L

A

A

K

M

V

I

N

H

-

V

R

K

R

R

G

C

V

T

P

P

V

I

R

P

A

A

I

L

L

L

I

-

G

-

T

-

V

-

F

F

G

T

Y

C

A

I

S

S

V

T

V

L

M

L

S

M

Y

L

V

V

S

T

P

S

D

D

T

-

V

-

F

M

A

Y

F

T

L

L

V

I

N

N

S

Y

Y

V

G

G

T

F

V

I

A
x
N

I
x
Y

F

L

V

F

Y

Y

V

G

L

V

I
x
T

A

V

I

A

S

G

Q

Q

L

I

K

V

L

L

R

R

A

W

R

K

I

-

E

K

R

P

D

D

A

I

P

P

E
x
R

K

P

L

I

R

K

V

I

R

N

M

L

I53 (≠ V27) binding
Y93 (≠ L65) mutation to A: Nearly complete reduction of glycine, L-alanine, and L-glutamine uptake. Minimal effect on the transport of L-isoleucine, L-histidine and L-tryptophan.
N134 (≠ V114) Important for substrate specificity; binding ; mutation to Q: Reduces L-leucine uptake activity. Abolishes L-tryptophan uptake.; mutation to S: The substrate specificity changed dramatically reducing L-glutamine, glycine and L-alanine uptake activity thus mimicking the selectivity of SLC7A5.
C154 (vs. gap) modified: Interchain (with C-210 in SLC3A2)
W174 (≠ A145) mutation to A: Does not affect protein expression, plasma membrane localization, or L-alanine uptake.
F243 (≠ G220) mutation to A: Abolishes leucine and tryptophan transport activities.
G246 (≠ F223) Important for substrate specificity; binding ; mutation to S: Strong decrease in the uptake of large substrates L-tryptophan, L-glutamine, and L-histidine but increases the uptake of small neutral amino acids glycine and L-alanine.
V302 (≠ M275) to I: found in a patient with age-related hearing loss; does not affect L-alanine transport activity. Decreases L-tyrosine transport activity
N395 (≠ A377) binding ; mutation to Q: Strongly reduces L-leucine uptake activity. Strongly reduces L-tryptophan uptake activity.
Y396 (≠ I378) mutation to A: Strongly reduces L-leucine uptake activity.
T402 (≠ I384) to M: found in a patient with age-related hearing loss; strongly decreased L-alanine transport activity. Decreases L-tyrosine transport activity
R418 (≠ E401) to C: found in a patient with age-related hearing loss; decreases L-alanine transport activity. Decreases L-tyrosine transport activity

Sites not aligning to the query:

460 V → E: found in a patient with age-related hearing loss; strongly decreases L-alanine transport activity. Decreases L-tyrosine transport activity. Decreases cell membrane localization

7cmiB The lat2-4f2hc complex in complex with leucine (see paper)
24% identity, 81% coverage: 22:407/476 of query aligns to 8:384/458 of 7cmiB

query
sites
7cmiB

I

I

A

I

L

V

G

G

G

N

V
x
I

I

I

G
|
G

A

S

G
|
G

L

I

F

F

V

V

G

S

S

P

G

K

V

G

V

V

V

L

Q

E

Q

N

A

A

G

G

P

S

A

V

-

G

-

L

A

A

V

L

L

I

S

V

F

W

L

I

I

V

T

T

G

G

A

F

L

I

V

T

V

V

L

V

V

G

M

A

R

L

M

C

L

Y

G

A

E

E

M

L

A

G

C

V

A

T

M

I

P

P

A

K

V

S

G

G

S

G

F

D

Y

Y

E

S

Y

Y

A

V

R

K

L

D

A

I

F

F

G

G

K

-

R

-

A

-

S

-

G

-

N

-

L

L

A

A

G

G

F

F

L

L

T

R

G

L

W

W

M

I

Y

A

W

-

Y

-

F

V

W

L

V

V

I

I

V

Y

V

P

A

T

V
x
N

E

Q

A

A

V

V

A

I

G

A

A

L

K

T

L

F

V

S

Q

N

F

Y

W

V

L

L

-

Q

-

P

-

L

-

F

-

P

-

T

-

C

-

F

-

P

P

P

D

E

V

S

P

G

A

L

W

R

A

L

I

L

S

A

L

A

V

I

L

C

L

L

V

L

T

L

L

L

T

T

A

W

T

V

N

N

L

C

V

S

S

S

V

V

G

R

S

W

Y

A

G

T

E

R

F

V

E

Q

F

D

W

I

F

F

A

T

S

A

I

G

K

K

V

L

A

L

A

A

I

L

M

A

V

L

F

I

L

I

F

I

L

M

G

G

G

I

M

V

Y

Q

V

I

L

C

G

K

-

G

-

E

-

Y

L

F

W

W

P

L

A

E

A

P

K

K

H

N

V

-

T

-

A

-

V

-

L

-

P

-

T

-

L

-

S

A

H

F

G

E

G

N

L

F

M

Q

P

E

K

P

G

D

I

I

G

G

P

L

V

V

L

A

S

L

G

A

A

F

V

L

A

Q

A

G

T

S

G
x
F

F

A

Y

Y

F
x
G

G

G

A

W

E

N

I

F

V

L

T

N

I

Y

A

V

A

T

A

E

E

E

A

L

Q

V

E

D

P

P

A

Y

K

K

A

N

V

L

A

P

K

R

A

A

T

I

N

F

S

I

V

S

I

I

T

P

R

L

V

V

L

T

V

F

F

V

Y

Y

V

V

-

F

G

A

S

N

I

V

L

A

L

Y

V

V

V

T

A

A

L

M

V

S

P

P

W

Q

N

E

-

L

-

A

S

S

P

N

K

A

M

V

A

A

T

V

P

T

Y

F

V

G

S

E

A

K

L

L

D

-

A

-

M

L

G

G

I

V

P

-

A

-

A

M

A

A

S

W

V

I

M

M

N

P

A

I

I

S

V

V

L

A

T

L

A

S

V

T

L

F

S

G

A

G

L

V

N

N

S

G

G

S

L

L

Y

F

A

T

A

S

S

S

R

R

M

L

I

F

F

F

A

A

L

G

T

A

R

R

H

E

G

G

D

H

A

L

P

P

A

S

A

V

L

L

A

A

K

M

V

I

N

H

-

V

R

K

R

R

G

C

V

T

P

P

V

I

R

P

A

A

I

L

L

L

I

-

G

-

T

-

V

-

F

F

G

T

Y

C

A

I

S

S

V

T

V

L

M

L

S

M

Y

L

V

V

S

T

P

S

D

D

T

M

V

Y

F

-

A

-

F

T

L

L

V

I

N

N

S

Y

Y

V

G

G

T

F

V

I

A

N

I

Y

F

L

V

F

Y

Y

V

G

L

V

I

T

A

V

I

A

S

G

Q

Q

L

I

K

V

L

L

R

R

A

W

R

K

I

-

E

K

R

P

D

D

A

I

P

P

E

R

K

P

L

I

R

K

V

I

R

N

M

L

binding leucine: I13 (≠ V27), G15 (= G29), G17 (= G31), N94 (≠ V114), F203 (≠ G220), G206 (≠ F223)

7cmhB The lat2-4f2hc complex in complex with tryptophan (see paper)
24% identity, 81% coverage: 22:407/476 of query aligns to 8:384/458 of 7cmhB

query
sites
7cmhB

I

I

A

I

L

V

G

G

G

N

V

I

I

I

G
|
G

A

S

G
|
G

L

I

F

F

V

V

G

S

S

P

G

K

V

G

V

V

V

L

Q

E

Q

N

A

A

G

G

P

S

A

V

-

G

-

L

A

A

V

L

L

I

S

V

F

W

L

I

I

V

T

T

G

G

A

F

L

I

V

T

V

V

L

V

V

G

M

A

R

L

M

C

L

Y

G

A

E

E

M

L

A

G

C

V

A

T

M

I

P

P

A

K

V

S

G

G

S

G

F

D

Y

Y

E

S

Y

Y

A

V

R

K

L

D

A

I

F

F

G

G

K

-

R

-

A

-

S

-

G

-

N

-

L

L

A

A

G

G

F

F

L

L

T

R

G

L

W

W

M

I

Y

A

W

-

Y

-

F

V

W

L

V

V

I

I

V

Y

V

P

A

T

V
x
N

E

Q

A

A

V

V

A

I

G

A

A

L

K

T

L

F

V

S

Q

N

F

Y

W

V

L

L

-

Q

-

P

-

L

-

F

-

P

-

T

-

C

-

F

-

P

P

P

D

E

V

S

P

G

A

L

W

R

A

L

I

L

S

A

L

A

V

I

L

C

L

L

V

L

T

L

L

L

T

T

A

W

T

V

N

N

L

C

V

S

S

S

V

V

G

R

S

W

Y

A

G

T

E

R

F

V

E

Q

F

D

W

I

F

F

A

T

S

A

I

G

K

K

V

L

A

L

A

A

I

L

M

A

V

L

F

I

L

I

F

I

L

M

G

G

G

I

M

V

Y

Q

V

I

L

C

G

K

-

G

-

E

-

Y

L

F

W

W

P

L

A

E

A

P

K

K

H

N

V

-

T

-

A

-

V

-

L

-

P

-

T

-

L

-

S

A

H

F

G

E

G

N

L

F

M

Q

P

E

K

P

G

D

I

I

G

G

P

L

V

V

L

A

S

L

G

A

A

F

V

L

A

Q

A

G

T

S

G
x
F

F
x
A

Y

Y

F
x
G

G

G

A

W

E

N

I

F

V

L

T

N

I

Y

A

V

A

T

A

E

E

E

A

L

Q

V

E

D

P

P

A

Y

K

K

A

N

V

L

A

P

K

R

A

A

T

I

N

F

S

I

V

S

I

I

T

P

R

L

V

V

L

T

V

F

F

V

Y

Y

V

V

-

F

G

A

S

N

I

V

L

A

L

Y

V

V

V

T

A

A

L

M

V

S

P

P

W

Q

N

E

-

L

-

A

S

S

P

N

K

A

M

V

A

A

T

V

P

T

Y

F

V

G

S

E

A

K

L

L

D

-

A

-

M

L

G

G

I

V

P

-

A

-

A

M

A

A

S

W

V

I

M

M

N

P

A

I

I

S

V

V

L

A

T

L

A

S

V

T

L

F

S

G

A

G

L

V

N

N

S

G

G

S

L

L

Y

F

A

T

A

S

S

S

R

R

M

L

I

F

F

F

A

A

L

G

T

A

R

R

H

E

G

G

D

H

A

L

P

P

A

S

A

V

L

L

A

A

K

M

V

I

N

H

-

V

R

K

R

R

G

C

V

T

P

P

V

I

R

P

A

A

I

L

L

L

I

-

G

-

T

-

V

-

F

F

G

T

Y

C

A

I

S

S

V

T

V

L

M

L

S

M

Y

L

V

V

S

T

P

S

D

D

T

M

V

Y

F

-

A

-

F

T

L

L

V

I

N

N

S

Y

Y

V

G

G

T

F

V

I

A
x
N

I

Y

F

L

V

F

Y

Y

V

G

L

V

I

T

A

V

I

A

S

G

Q

Q

L

I

K

V

L

L

R

R

A

W

R

K

I

-

E

K

R

P

D

D

A

I

P

P

E

R

K

P

L

I

R

K

V

I

R

N

M

L

binding tryptophan: G15 (= G29), G17 (= G31), N94 (≠ V114), F203 (≠ G220), A204 (≠ F221), G206 (≠ F223), N355 (≠ A377)

7b00A Human lat2-4f2hc complex in the apo-state (see paper)
24% identity, 81% coverage: 22:407/476 of query aligns to 8:384/457 of 7b00A

query
sites
7b00A

I

I

A

I

L

V

G

G

G

N

V

I

I

I

G

G

A

S

G

G

L

I

F

F

V

V

G

S

S

P

G

K

V

G

V

V

V

L

Q

E

Q

N

A

A

G

G

P

S

A

V

-

G

-

L

A

A

V

L

L

I

S

V

F

W

L

I

I

V

T

T

G

G

A

F

L

I

V

T

V

V

L

V

V

G

M

A

R

L

M

C

L

Y

G

A

E

E

M

L

A

G

C

V

A

T

M

I

P

P

A

K

V

S

G

G

S

G

F

D

Y

Y

E

S

Y

Y

A

V

R

K

L

D

A

I

F

F

G

G

K

-

R

-

A

-

S

-

G

-

N

-

L

L

A

A

G

G

F

F

L

L

T

R

G

L

W

W

M

I

Y

A

W

-

Y

-

F

V

W

L

V

V

I

I

V

Y

V

P

A

T

V

N

E

Q

A

A

V

V

A

I

G

A

A

L

K

T

L

F

V

S

Q

N

F

Y

W

V

L

L

-

Q

-

P

-

L

-

F

-

P

-

T

-

C

-

F

-

P

P

P

D

E

V

S

P

G

A

L

W

R

A

L

I

L

S

A

L

A

V

I

L

C

L

L

V

L

T

L

L

L

T

T

A

W

T

V

N

N

L

C

V

S

S

S

V

V

G

R

S

W

Y

A

G

T

E

R

F

V

E

Q

F

D

W

I

F

F

A

T

S

A

I

G

K

K

V

L

A

L

A

A

I

L

M

A

V

L

F

I

L

I

F

I

L

M

G

G

G

I

M

V

Y

Q

V

I

L

C

G

K

-

G

-

E

-

Y

L

F

W

W

P

L

A

E

A

P

K

K

H

N

V

-

T

-

A

-

V

-

L

-

P

-

T

-

L

-

S

A

H

F

G

E

G

N

L

F

M

Q

P

E

K

P

G

D

I

I

G

G

P

L

V

V

L

A

S

L

G

A

A

F

V

L

A

Q

A

G

T

S

G

F

F

A

Y

Y

F

G

G

G

A

W

E

N

I

F

V

L

T

N

I

Y

A

V

A

T

A

E

E

E

A

L

Q

V

E

D

P

P

A

Y

K

K

A

N

V

L

A

P

K

R

A

A

T

I

N

F

S

I

V

S

I

I

T

P

R

L

V

V

L

T

V

F

F

V

Y

Y

V

V

-

F

G

A

S

N

I

V

L

A

L

Y

V

V

V

T

A

A

L

M

V

S

P

P

W

Q

N

E

-

L

-

A

S

S

P

N

K

A

M

V

A

A

T

V

P

T

Y

F

V

G

S

E

A

K

L

L

D

-

A

-

M

L

G

G

I

V

P

-

A

-

A

M

A

A

S

W

V

I

M

M

N

P

A

I

I

S

V

V

L

A

T

L

A

S

V

T

L

F

S

G

A

G

L

V

N

N

S

G

G

S

L

L

Y

F

A

T

A

S

S

S

R

R

M

L

I

F

F

F

A

A

L

G

T

A

R

R

H

E

G

G

D

H

A

L

P

P

A

S

A

V

L

L

A

A

K

M

V

I

N

H

-

V

R

K

R

R

G

C

V

T

P

P

V

I

R

P

A

A

I

L

L

L

I

-

G

-

T

-

V

-

F

F

G

T

Y

C

A

I

S

S

V

T

V

L

M

L

S
x
M

Y

L

V

V

S

T

P

S

D

D

T

M

V

Y

F

-

A

-

F

T

L

L

V

I

N

N

S
x
Y

Y

V

G

G

T

F

V

I

A

N

I

Y

F

L

V

F

Y

Y

V

G

L

V

I

T

A

V

I

A

S

G

Q

Q

L

I

K

V

L

L

R

R

A

W

R

K

I

-

E

K

R

P

D

D

A

I

P

P

E

R

K

P

L

I

R

K

V

I

R

N

M

L

binding Digitonin: M338 (≠ S358), Y350 (≠ S372)

Sites not aligning to the query:

binding Digitonin: 408, 411

Q9QXW9 Large neutral amino acids transporter small subunit 2; L-type amino acid transporter 2; mLAT2; Solute carrier family 7 member 8 from Mus musculus (Mouse) (see paper)
24% identity, 81% coverage: 22:407/476 of query aligns to 47:423/531 of Q9QXW9

query
sites
Q9QXW9

I

I

A

I

L

V

G

G

G

N

V

I

I

I

G

G

A

S

G

G

L

I

F

F

V

V

G

S

S

P

G

K

V

G

V

V

V

L

Q

E

Q

N

A

A

G

G

P

S

A

V

-

G

-

L

A

A

V

L

L

I

S

V

F

W

L

I

I

V

T

T

G

G

A

I

L

I

V

T

V

A

L

V

V

G

M

A

R

L

M

C

L

Y

G

A

E

E

M

L

A

G

C

V

A

T

M

I

P

P

A

K

V

S

G

G

S

G

F

D

Y

Y

E

S

Y

Y

A

V

R

K

L

D

A

I

F

F

G

G

K

-

R

-

A

-

S

-

G

-

N

-

L

L

A

A

G

G

F

F

L

L

T

R

G

L

W

W

M

I

Y

A

W

-

Y

-

F

V

W

L

V

V

I

I

V
x
Y

V

P

A

T

V
x
N

E

Q

A

A

V

V

A

I

G

A

A

L

K

T

L

F

V

S

Q

N

F

Y

W

V

L

L

-

Q

-

P

-

L

-

F

-

P

-

T

-

C

-

F

-

P

P

P

D

E

V

S

P

G

A

L

W

R

A

L

I

L

S

A

L

A

V

I

L

C

L

L

V

L

T

L

L

L

T

T

A

W

T

V

N

N

L

C

V

S

S

S

V

V

G

R

S

W

Y

A

G

T

E

R

F

V

E

Q

F

D

W

I

F

F

A

T

S

A

I

G

K

K

V

L

A

L

A

A

I

L

M

A

V

L

F

I

L

I

F

I

L

M

G

G

G

I

M

V

Y

Q

V

I

L

C

G

K

-

G

-

E

-

F

L

F

W

W

P

L

A

E

A

P

K

K

H

N

V

-

T

-

A

-

V

-

L

-

P

-

T

-

L

-

S

A

H

F

G

E

G

N

L

F

M

Q

P

E

K

P

G

D

I

I

G

G

P

L

V

V

L

A

S

L

G

A

A

F

V

L

A

Q

A

G

T

S

G
x
F

F

A

Y

Y

F

G

G

G

A

W

E

N

I

F

V

L

T

N

I

Y

A

V

A

T

A

E

E

E

A

L

Q

V

E

D

P

P

A

Y

K

K

A

N

V

L

A

P

K

R

A

A

T

I

N

F

S

I

V

S

I

I

T

P

R

L

V

V

L

T

V

F

F

V

Y

Y

V

V

-

F

G

A

S

N

I

I

L

A

L

Y

V

V

V

T

A

A

L

M

V

S

P

P

W

Q

N

E

-

L

-

A

S

S

P

N

K

A

M

V

A

A

T

V

P

T

Y

F

V

G

S

E

A

K

L

L

D

-

A

-

M

L

G

G

I

V

P

-

A

-

A

M

A

A

S

W

V

I

M

M

N

P

A

I

I

S

V

V

L

A

T

L

A

S

V

T

L

F

S

G

A

G

L

V

N

N

S

G

G

S

L

L

Y

F

A

T

A

S

S

S

R

R

M

L

I

F

F

F

A

A

L

G

T

A

R

R

H

E

G

G

D

H

A

L

P

P

A

S

A

V

L

L

A

A

K

M

V

I

N

H

-

V

R

K

R

R

G

C

V

T

P

P

V

I

R

P

A

A

I

L

L

L

I

-

G

-

T

-

V

-

F

F

G

T

Y

C

A

L

S

S

V

T

V

L

M

L

S

M

Y

L

V

V

S

T

P

S

D

D

T

-

V

-

F

M

A

Y

F

T

L

L

V

I

N

N

S

Y

Y

V

G

G

T

F

V

I

A

N

I

Y

F

L

V

F

Y

Y

V

G

L

V

I

T

A

V

I

A

S

G

Q

Q

L

I

K

V

L

L

R

R

A

W

R

K

I

-

E

K

R

P

D

D

A

I

P

P

E

R

K

P

L

I

R

K

V

V

R

S

M

L

Y130 (≠ V111) mutation to A: Increases T2 import. Increases T3 and enables T4 import. Does not affect L-leucine and L-phenylalanine uptake.
N133 (≠ V114) mutation to S: Increases T2 import. Does not affect T3 import. Does not affect L-leucine and L-phenylalanine uptake. Increases the export of both L-leucine and L-phenylalanine.
F242 (≠ G220) mutation to W: Increases T2 import. Does not affect T3 import. Does not affect L-leucine and L-phenylalanine uptake.

P76037 Putrescine importer PuuP from Escherichia coli (strain K12) (see paper)
22% identity, 67% coverage: 8:328/476 of query aligns to 16:326/461 of P76037

query
sites
P76037

T

T

E

R

L

L

K

R

A

K

G

S

L

L

K

K

Q

L

R

W

H

Q

M

V

T

V

M

M

I

M

A

G

L

L

G

A

G

Y

V

L

I

T

G

P

A

M

G

T

L

V

F

F

V

D

G

T

S

F

G

G

V

I

V

V

V

S

Q

G

Q

I

A

S

G

D

P

G

A

H

A

V

V

P

L

A

S

S

F

Y

L

L

I

L

T

A

G

L

A

A

L

G

V

V

V

L

L

F

V

T

M

A

R

I

M

S

L

Y

G

G

E

K

M

L

A

V

C

R

A

Q

M

F

P

P

A

E

V

A

G

G

S

S

F

A

Y

Y

E

T

Y

Y

A

A

R

-

L

-

A

-

F

-

G

-

K

Q

R

K

A

S

S

I

G

N

N

P

L

H

A

V

G

G

F

F

L

M

T

V

G

G

W

W

M

S

Y

S

W

L

Y

L

F

D

W
x
Y

V

L

I

F

V

L

V

P

A

M

V

I

E

N

A

V

V

L

A

L

G

A

A

K

K

I

L

Y

V

L

Q

S

F

A

W

L

L

F

P

P

D

E

V

V

P

P

A

P

W

W

A

V

I

W

S

V

L

V

V

T

L

F

L

V

V

A

T

I

L

L

T

T

A

A

T

A

N

N

L

L

V

K

S

S

V

V

G

N

S

L

Y

V

G

A

E

N

F

F

E

N

F

T

W

L

F

F

A

V

S

L

I

V

K

Q

V

I

A

S

A

I

I

M

M

V

V

V

F

F

L

I

F

F

L

L

G

-

M

-

Y

V

V

V

L

Q

G

G

L

L

W

H

P

K

A

G

A

E

-

G

-

V

K

G

H

T

V

V

T

W

A

S

V

L

L

Q

P

P

T

F

L

I

L

S

S

E

H

N

G

A

G

H

L

L

M

I

P

-

K

-

G

-

I

-

G

-

P

P

V

I

L

I

S

T

G

G

A

A

V

T

A

I

A

V

T

C

G

F

F

S

Y

F

F

L

G

G

A

F

E

D

I

A

V

V

T

T

I

T

A

L

A

S

A

E

E

E

A

T

Q

P

E

D

P

A

A

A

K

R

A

V

V

I

A

P

K

K

A

A

T

I

N

F

S

L

V

T

I

A

T

V

R

Y

V

G

L

G

V

V

F

I

Y

F

V

I

G

A

S

A

I

S

L

F

V

M

V

Q

A

L

L

F

V

F

P

P

W

-

N

D

S

I

P

S

K

R

M

F

A

K

T

D

P

P

Y

D

V

-

S

A

A

A

L

L

D

P

A

E

M

I

G

A

I

L

P

Y

A

V

A

G

S

K

V

L

M

F

N

Q

A

S

I

I

V

F

L

L

-

C

T

T

A

T

V

F

L

V

S

N

A

T

L

L

N

A

S

S

G

G

L

L

-

A

-

S

-

H

Y

A

A

S

A

V

S

S

R

R

M

L

I

L

F

Y

A

V

L

M

T

G

R

R

H

D

G

N

D

V

A

F

P

P

Y110 (≠ W108) mutation to X: The uptake activity is reduced to one-eighth of that of wild-type.

P30825 High affinity cationic amino acid transporter 1; CAT-1; CAT1; Ecotropic retroviral leukemia receptor homolog; Ecotropic retrovirus receptor homolog; Solute carrier family 7 member 1; System Y+ basic amino acid transporter from Homo sapiens (Human) (see paper)
35% identity, 21% coverage: 4:102/476 of query aligns to 22:115/629 of P30825

query
sites
P30825

S

S

T

R

D

E

T

E

T

T

E

R

L

L

K

S

A

R

G

C

L

L

K

N

Q

T

R

F

H

D

M

L

T

V

M

A

I

L

A

G

L

V

G

G

G

S

V

T

I

L

G

G

A

A

G

G

L

V

F

Y

V

V

G

L

S

A

G

G

V

A

V

V

V

A

Q

R

Q

E

-

N

A

A

G

G

P

P

A

A

A

I

V

V

L

I

S

S

F

F

L

L

I

I

T

A

G

A

A

L

L

A

V

S

V

V

L

L

V

A

M

G

R

L

M

C

L

Y

G

G

E

E

M

F

A

G

C

A

A

R

M

V

P

P

A

K

V

T

G

G

S

S

F

A

Y

Y

E

L

Y

Y

A

S

R

Y

L

V

A

T

F

V

G

-

K

-

R

-

A

-

S

-

G

G

N

E

L

L

A

W

G

A

F

F

L

I

T

T

G

G

W

W

Sites not aligning to the query:

226 modified: carbohydrate, N-linked (GlcNAc...) asparagine

O34739 Serine/threonine exchanger SteT from Bacillus subtilis (strain 168) (see paper)
21% identity, 87% coverage: 5:420/476 of query aligns to 3:399/438 of O34739

query
sites
O34739

T

T

D

E

T

D

T

N

E

G

L

L

K

K

A

K

G

E

L

I

K

G

Q

L

R

L

H

F

M

A

T

L

M

T

I

L

A

V

L

I

G

G

G

T

V

I

I

I

G

G

A

S

G

G

L

V

F

F

V

M

G

K

S

P

G

G

V

A

V

V

V

L

Q

A

Q

Y

A

S

G

G

P

D

A

S

-

K

-

M

A

A

V

L

L

F

S

A

F

W

L

L

I

L

T

G

G

G

A

I

L

L

V

T

V

L

L

A

V

G

M

G

R

L

M

T

L

I

G

A

E

E

M

I

A

G

C

T

A

Q

M

I

P

P

A

K

V

T

G

G

S

G

F

L

Y

Y

E

T

Y

Y

A

L

R

-

L

-

A

-

F

-

G

-

K

E

R

E

A

V

S

Y

G

G

N

E

L

F

A

W

G

G

F

F

L

L

T
x
C

G

G

W

W

M

V

Y

Q

W

-

Y

-

F

-

W

-

V

-

I

I

V

I

A

Y

V

G

E

P

A

A

V

I

A

I

G

G

A

A

K

L

L

G

V

L

Q

Y

F

F

-

G

-

S

W

L

L

M

P

A

D

N

V

L

P

F

A

G

W

W

A

G

-

S

-

G

-

L

-

S

-

K

-

V

I

I

S

G

L

I

V

I

L

A

L

V

V

L

T

F

L

L

T
x
C

A

V

T

I

N

N

L

I

V

I

S

G

V

T

G

-

S

K

Y

Y

G

G

E

G

F

F

E

V

F

Q

W

T

F

L

A

T

S

T

-

I

-

G

-

K

-

L

I

I

K

P

V

I

A

A

A
x
C

I

I

M

I

V

V

F

F

L

-

F

-

L

-

G

-

M

-

Y

-

V

-

L

-

G

G

L

L

W

W

P

K

A

G

A

D

K

Q

H

H

V

I

-

F

T

T

A

A

V

V

L

N

P

E

T

S

L

I

L

S

S

D

H

M

G

N

G

-

L

-

M

-

P

-

K

-

G

-

I

-

G

-

P

-

V

-

L

F

S

G

G

A

A

A

V

I

A

L

A

A

T

T

G

L

F

F

Y

A

F

Y

G

D

A

G

E

W

I

I

V

L

T

L

I

A

A

A

-

L

A

G

E

E

A

M

Q

K

E

N

P

P

A

E

K

K

A

L

V

L

A

P

K

R

A

A

T

M

N

T

S

G

-

G

-

L

V

I

I

V

T

T

R

A

V

I

L

Y

V

I

F

F

Y

I

V

N

G

F

S

A

I

L

L

L

L

H

V

I

V

L

A

S

-

A

-

N

-

E

L

I

V

V

P

T

W

L

N

G

S

E

P

N

K

A

M

T

A

S

T

T

P

A

Y

A

V

T

S

M

A

L

L

F

D

G

A

S

M

I

G

G

I

-

P

-

A

-

A

G

S

K

V

L

M

I

N

S

A

V

I

G

V

I

L

I

T

V

A

S

V

I

L

F

S

G

A
x
C

L

L

N

N

S

G

G

K

L

V

Y

L

A

S

A

F

S

P

R

R

M

V

I

S

F

F

A

A

L

M

T

A

R

E

H

R

G

K

D

Q

A

L

P

P

A

F

A

A

L

-

A

E

K

K

V

L

N

S

R

H

R

V

G

H

V

P

P

S

V

F

R

R

A

T

-

P

-

W

I

I

L

A

I

I

G

S

T

F

V

Q

F

I

G

A

Y

L

A

A

S

L

V

I

V

M

M

M

S

L

Y

I

V

S

S

N

P

P

D

D

T

K

V

L

F

S

A

E

F

-

L

-

V

I

N

S

S

I

Y

F

G

M

T

I

V

Y

A

I

I

F

F

Y

V

V

Y

M

V

A

L

F

I

F

A

A

I

V

S

F

Q

I

L

L

K

R

L

K

R

R

A

A

R

K

I

G

E

E

R

K

D

R

A

A

P

-

E

-

K

-

L

-

R

-

V

Y

R

S

M

V

W

P

C

L

Y

Y

P

P

Y

F

L

M

T

P

W

I

V

L

A

A

I

I

I

A

G

G

C94 (≠ T100) mutation to S: Retains 25% of the transport activity; when associated with S-141; S-168; S-291 and S-415.
C141 (≠ T144) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-168; S-291 and S-415.
C168 (≠ A168) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-141; S-291 and S-415.
C291 (≠ A306) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-415.

Sites not aligning to the query:

415 C→S: Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-291.

Query Sequence

>BPHYT_RS21680 BPHYT_RS21680 amino acid permease-associated protein
MQRSTDTTELKAGLKQRHMTMIALGGVIGAGLFVGSGVVVQQAGPAAVLSFLITGALVVL
VMRMLGEMACAMPAVGSFYEYARLAFGGKRASGNLAGFLTGWMYWYFWVIVVAVEAVAGA
KLVQFWLPDVPAWAISLVLLVTLTATNLVSVGSYGEFEFWFASIKVAAIMVFLFLGGMYV
LGLWPAAKHVTAVLPTLLSHGGLMPKGIGPVLSGAVAATGFYFGAEIVTIAAAEAQEPAK
AVAKATNSVITRVLVFYVGSILLVVALVPWNSPKMATPYVSALDAMGIPAAASVMNAIVL
TAVLSALNSGLYAASRMIFALTRHGDAPAALAKVNRRGVPVRAILIGTVFGYASVVMSYV
SPDTVFAFLVNSYGTVAIFVYVLIAISQLKLRARIERDAPEKLRVRMWCYPYLTWVAIIG
MVGILVAMAFIPEQRQPLWFGVASLGVLLLAYLLRVRKPDAGFGEPDTLEYRHHHH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory