SitesBLAST
Comparing BPHYT_RS21745 FitnessBrowser__BFirm:BPHYT_RS21745 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
8b7sA Crystal structure of the chloramphenicol-inactivating oxidoreductase from novosphingobium sp (see paper)
35% identity, 97% coverage: 2:534/551 of query aligns to 2:454/458 of 8b7sA
- binding flavin-adenine dinucleotide: G11 (= G11), G13 (= G13), S14 (= S14), A15 (= A15), E35 (= E35), A36 (= A36), W47 (= W62), P65 (= P80), G67 (= G82), V180 (≠ A219), A214 (= A253), G215 (= G254), A218 (≠ Q257), T270 (= T309), Y391 (≠ F469), A424 (≠ C504), I435 (≠ T515), N436 (= N516)
5nccA Structure of fatty acid photodecarboxylase in complex with fad and palmitic acid (see paper)
36% identity, 97% coverage: 4:535/551 of query aligns to 23:574/578 of 5nccA
- active site: R347 (= R319), L420 (≠ I383), I421 (≠ C384), S507 (≠ I468), A509 (≠ H470), G552 (= G513), Q553 (≠ N514)
- binding flavin-adenine dinucleotide: G30 (= G11), G32 (= G13), T33 (≠ S14), A34 (= A15), L53 (= L34), E54 (= E35), A55 (= A36), F74 (= F56), W80 (= W62), A98 (≠ P80), G100 (= G82), G105 (= G87), S106 (= S88), N110 (= N92), A111 (≠ G93), T112 (≠ L94), L113 (≠ I95), V238 (≠ A219), A278 (= A253), H282 (≠ Q257), L286 (= L261), N508 (≠ F469), Q553 (≠ N514), T554 (= T515), G555 (≠ N516), V558 (≠ I519)
6yrvAAA structure of fap after illumination at 100k (see paper)
36% identity, 97% coverage: 4:535/551 of query aligns to 7:565/573 of 6yrvAAA
- binding carbon dioxide: R375 (≠ H360), N499 (≠ F469)
- binding flavin-adenine dinucleotide: G14 (= G11), G16 (= G13), T17 (≠ S14), A18 (= A15), L37 (= L34), E38 (= E35), A39 (= A36), F58 (= F56), W64 (= W62), A82 (≠ P80), G89 (= G87), S90 (= S88), N94 (= N92), A95 (≠ G93), T96 (≠ L94), L97 (≠ I95), M191 (≠ T188), V222 (≠ A219), C264 (≠ T252), A265 (= A253), G266 (= G254), H269 (≠ Q257), N499 (≠ F469), A534 (≠ C504), Q544 (≠ N514), T545 (= T515), G546 (≠ N516)
- binding heptadecane: V377 (vs. gap), G379 (≠ T363), M380 (≠ L364), G386 (= G370), T389 (≠ V373), Y390 (≠ H374), F393 (vs. gap), T408 (= T380), Q410 (≠ S382)
A0A248QE08 Fatty acid photodecarboxylase, chloroplastic; CvFAP; EC 4.1.1.106 from Chlorella variabilis (Green alga) (see paper)
36% identity, 97% coverage: 4:535/551 of query aligns to 83:641/654 of A0A248QE08
- TA 93:94 (≠ SA 14:15) binding
- E114 (= E35) binding
- L162 (≠ T84) binding
- S166 (= S88) binding
- NATL 170:173 (≠ NGLI 92:95) binding
- V298 (≠ A219) binding
- C432 (≠ I338) binding
- R451 (≠ H360) binding
- Y466 (≠ H374) binding
- Q486 (≠ S382) binding
- G622 (≠ N516) binding
2jbvA Crystal structure of choline oxidase reveals insights into the catalytic mechanism (see paper)
34% identity, 96% coverage: 1:530/551 of query aligns to 9:526/527 of 2jbvA
- active site: I333 (≠ L328), P377 (≠ I383), N378 (≠ C384), V464 (≠ I468), H466 (= H470), V509 (≠ G513), N510 (= N514)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[(4aS,10aR)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: G22 (= G13), S23 (= S14), E44 (= E35), A45 (= A36), W71 (= W62), A90 (≠ G82), G95 (= G87), C96 (≠ S88), H99 (≠ I91), N100 (= N92), S101 (≠ G93), I103 (= I95), R231 (≠ M218), A232 (= A219), T269 (≠ A253), G270 (= G254), D273 (≠ Q257), V464 (≠ I468), Y465 (≠ F469), H466 (= H470), D499 (= D503), A500 (≠ C504), N510 (= N514), P511 (≠ T515), N512 (= N516), V515 (≠ I519)
4mjwA Crystal structure of choline oxidase in complex with the reaction product glycine betaine (see paper)
34% identity, 96% coverage: 1:530/551 of query aligns to 9:526/532 of 4mjwA
- active site: I333 (≠ L328), P377 (≠ I383), N378 (≠ C384), V464 (≠ I468), H466 (= H470), V509 (≠ G513), N510 (= N514)
- binding flavin-adenine dinucleotide: G20 (= G11), G22 (= G13), S23 (= S14), E44 (= E35), A45 (= A36), W71 (= W62), R89 (= R81), A90 (≠ G82), G95 (= G87), C96 (≠ S88), H99 (≠ I91), N100 (= N92), S101 (≠ G93), I103 (= I95), R231 (≠ M218), A232 (= A219), T269 (≠ A253), G270 (= G254), D273 (≠ Q257), Y465 (≠ F469), H466 (= H470), A500 (≠ C504), N510 (= N514), P511 (≠ T515), N512 (= N516), V515 (≠ I519)
3ljpA Crystal structure of choline oxidase v464a mutant (see paper)
34% identity, 96% coverage: 1:530/551 of query aligns to 9:526/530 of 3ljpA
- active site: I333 (≠ L328), P377 (≠ I383), N378 (≠ C384), A464 (≠ I468), H466 (= H470), V509 (≠ G513), N510 (= N514)
- binding dihydroflavine-adenine dinucleotide: G22 (= G13), S23 (= S14), E44 (= E35), A45 (= A36), W71 (= W62), R89 (= R81), A90 (≠ G82), G95 (= G87), C96 (≠ S88), H99 (≠ I91), N100 (= N92), S101 (≠ G93), I103 (= I95), A232 (= A219), T269 (≠ A253), D273 (≠ Q257), Y465 (≠ F469), H466 (= H470), D499 (= D503), A500 (≠ C504), N510 (= N514), P511 (≠ T515), N512 (= N516), V515 (≠ I519)
5oc1A Crystal structure of aryl-alcohol oxidase from pleurotus eryngii in complex with p-anisic acid (see paper)
32% identity, 96% coverage: 5:533/551 of query aligns to 2:564/565 of 5oc1A
- active site: V339 (≠ K321), N413 (≠ Q385), A414 (vs. gap), I499 (= I468), H501 (= H470), A544 (≠ G513), H545 (≠ N514)
- binding 4-methoxybenzoic acid: Y91 (≠ G93), I356 (= I338), I390 (≠ L364), F396 (≠ G370), T412 (≠ C384), I499 (= I468), H501 (= H470), H545 (≠ N514)
- binding flavin-adenine dinucleotide: G8 (= G11), G10 (= G13), N11 (≠ S14), A12 (= A15), E32 (= E35), A33 (= A36), W60 (= W62), P78 (= P80), G80 (= G82), G85 (= G87), S86 (= S88), H90 (≠ N92), Y91 (≠ G93), V93 (≠ I95), V230 (≠ A219), S270 (≠ T252), A271 (= A253), G272 (= G254), F500 (= F469), H545 (≠ N514), T546 (= T515), Q547 (≠ N516), I550 (= I519)
3fimB Crystal structure of aryl-alcohol-oxidase from pleurotus eryingii (see paper)
31% identity, 96% coverage: 5:533/551 of query aligns to 2:564/565 of 3fimB
- active site: V339 (≠ K321), N413 (≠ Q385), A414 (vs. gap), I499 (= I468), H501 (= H470), A544 (≠ G513), H545 (≠ N514)
- binding flavin-adenine dinucleotide: G8 (= G11), N11 (≠ S14), A12 (= A15), E32 (= E35), A33 (= A36), W60 (= W62), P78 (= P80), G80 (= G82), G85 (= G87), S86 (= S88), H90 (≠ N92), Y91 (≠ G93), V93 (≠ I95), V230 (≠ A219), S270 (≠ T252), A271 (= A253), F500 (= F469), H501 (= H470), H545 (≠ N514), T546 (= T515), Q547 (≠ N516), I550 (= I519)
E4QP00 5-(hydroxymethyl)furfural oxidase; 5-hydroxymethylfurfural oxidase; HMFO; Thiol oxidase; EC 1.1.3.47; EC 1.8.3.- from Methylovorus sp. (strain MP688) (see paper)
35% identity, 96% coverage: 5:534/551 of query aligns to 6:531/531 of E4QP00
- V101 (≠ I91) mutation to H: Abolishes activity.
- M103 (≠ G93) mutation to A: 16-fold reduction in catalytic efficiency on vanillyl alcohol.
- V367 (≠ T380) mutation to K: 1.6-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion.; mutation to R: 1.4-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with F-466.
- W369 (≠ S382) mutation to A: 7.5-fold reduction in catalytic efficiency on vanillyl alcohol.
- V465 (≠ I468) mutation to A: 18-fold reduction in catalytic efficiency on vanillyl alcohol.
- W466 (≠ F469) mutation to A: 39-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate.; mutation to F: 3.4-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with R-367.
- H467 (= H470) mutation to A: Abolishes activity.
- N511 (= N514) mutation to A: 53-fold reduction in catalytic efficiency on vanillyl alcohol.
4udqA Crystal structure of 5-hydroxymethylfurfural oxidase (hmfo) in the reduced state
35% identity, 96% coverage: 5:531/551 of query aligns to 2:524/525 of 4udqA
- active site: L331 (≠ W326), F364 (≠ Y381), W365 (≠ S382), V461 (≠ I468), H463 (= H470), A506 (≠ G513), N507 (= N514)
- binding flavin-adenine dinucleotide: G8 (= G11), G10 (= G13), T11 (≠ S14), A12 (= A15), E32 (= E35), A33 (= A36), W64 (= W44), G88 (= G82), G93 (= G87), G94 (≠ S88), N98 (= N92), M99 (≠ G93), V101 (≠ I95), V229 (≠ A219), T261 (= T252), A262 (= A253), W462 (≠ F469), H463 (= H470), A497 (≠ C504), N507 (= N514), T508 (= T515), N509 (= N516), T512 (≠ I519)
8bxlB Patulin synthase from penicillium expansum
31% identity, 95% coverage: 5:525/551 of query aligns to 14:581/590 of 8bxlB
- binding flavin-adenine dinucleotide: G20 (= G11), G22 (= G13), T23 (≠ S14), A24 (= A15), E44 (= E35), A45 (= A36), W80 (= W62), G100 (= G82), G105 (= G87), S106 (= S88), R109 (≠ I91), N110 (= N92), Y111 (≠ G93), A113 (≠ I95), L253 (≠ M218), A254 (= A219), A288 (= A253), Q292 (= Q257), F525 (= F469), D559 (= D503), A560 (≠ C504), H570 (≠ N514), P571 (≠ T515), Q572 (≠ N516), L575 (≠ I519)
4ynuA Crystal structure of aspergillus flavus fadgdh in complex with d- glucono-1,5-lactone (see paper)
31% identity, 96% coverage: 5:534/551 of query aligns to 4:566/569 of 4ynuA
- active site: V341 (≠ Q312), F412 (vs. gap), W413 (vs. gap), N501 (≠ I468), H503 (= H470), G545 (= G513), H546 (≠ N514)
- binding flavin-adenine dinucleotide: G12 (= G13), T13 (≠ S14), S14 (≠ A15), E34 (= E35), A35 (= A36), Y51 (= Y51), F55 (= F56), W61 (= W62), R79 (≠ P80), G81 (= G82), G86 (= G87), T87 (≠ S88), N91 (= N92), G92 (= G93), T232 (≠ M218), A233 (= A219), A273 (= A253), G274 (= G254), R277 (≠ Q257), F502 (= F469), A536 (≠ C504), H546 (≠ N514), L547 (≠ T515), V548 (≠ N516), L551 (≠ I519)
- binding D-glucono-1,5-lactone: Y51 (= Y51), E411 (vs. gap), A496 (≠ E463), N497 (≠ Y464), R499 (≠ Q466), R499 (≠ Q466), N501 (≠ I468), H503 (= H470), H546 (≠ N514)
4yntA Crystal structure of aspergillus flavus fad glucose dehydrogenase (see paper)
31% identity, 96% coverage: 5:534/551 of query aligns to 5:567/570 of 4yntA
- active site: V342 (≠ Q312), F413 (vs. gap), W414 (vs. gap), N502 (≠ I468), H504 (= H470), G546 (= G513), H547 (≠ N514)
- binding dihydroflavine-adenine dinucleotide: G13 (= G13), T14 (≠ S14), S15 (≠ A15), E35 (= E35), A36 (= A36), F56 (= F56), W62 (= W62), R80 (≠ P80), G82 (= G82), G87 (= G87), T88 (≠ S88), N92 (= N92), G93 (= G93), M94 (≠ L94), A95 (≠ I95), A234 (= A219), A274 (= A253), R278 (≠ Q257), F503 (= F469), A537 (≠ C504), H547 (≠ N514), L548 (≠ T515), V549 (≠ N516), L552 (≠ I519)
7vzsA Fad-dpendent glucose dehydrogenase complexed with an inhibitor at ph7.56
31% identity, 96% coverage: 5:534/551 of query aligns to 4:566/566 of 7vzsA
- binding D-glucal: Y6 (≠ F7), L22 (= L23), N25 (≠ G26), Y51 (= Y51), I349 (≠ A320), Q356 (≠ A327), E411 (vs. gap), E444 (≠ D415), W445 (≠ L416), K448 (≠ R419), R499 (≠ Q466), N501 (≠ I468), H546 (≠ N514), K563 (≠ L531), A566 (= A534)
- binding flavin-adenine dinucleotide: G10 (= G11), G12 (= G13), T13 (≠ S14), S14 (≠ A15), E34 (= E35), A35 (= A36), Y51 (= Y51), F55 (= F56), W61 (= W62), R79 (≠ P80), G81 (= G82), G86 (= G87), T87 (≠ S88), N91 (= N92), G92 (= G93), M93 (≠ L94), A94 (≠ I95), T232 (≠ M218), A233 (= A219), A273 (= A253), G274 (= G254), R277 (≠ Q257), F502 (= F469), A536 (≠ C504), H546 (≠ N514), L547 (≠ T515), V548 (≠ N516), L551 (≠ I519)
7aa2A Chaetomium thermophilum fad-dependent oxidoreductase in complex with abts (see paper)
31% identity, 95% coverage: 5:530/551 of query aligns to 2:578/584 of 7aa2A
- binding 3-ethyl-2-[(2z)-2-(3-ethyl-6-sulfo-1,3-benzothiazol-2(3h)-ylidene)hydrazino]-6-sulfo-3h-1,3-benzothiazol-1-ium: W52 (vs. gap), A88 (≠ G93), V90 (≠ I95), L354 (≠ G332), Y431 (≠ C384), N517 (≠ I468), H519 (= H470), S562 (≠ N514)
- binding dihydroflavine-adenine dinucleotide: G8 (= G11), G10 (= G13), I11 (≠ S14), S12 (≠ A15), E32 (= E35), A33 (= A36), W56 (= W62), A77 (≠ G82), G82 (= G87), G83 (≠ S88), I86 (= I91), N87 (= N92), A88 (≠ G93), V90 (≠ I95), L227 (≠ M218), V228 (≠ A219), A265 (= A253), A518 (≠ F469), H519 (= H470), D551 (= D503), I552 (≠ C504), S562 (≠ N514), P563 (≠ T515), M564 (≠ N516)
6ze7B Chaetomium thermophilum fad-dependent oxidoreductase in complex with 4-nitrophenol (see paper)
31% identity, 95% coverage: 5:530/551 of query aligns to 3:579/586 of 6ze7B
- binding dihydroflavine-adenine dinucleotide: G9 (= G11), G11 (= G13), I12 (≠ S14), S13 (≠ A15), E33 (= E35), A34 (= A36), W57 (= W62), A78 (≠ G82), G83 (= G87), G84 (≠ S88), N88 (= N92), A89 (≠ G93), V91 (≠ I95), L228 (≠ M218), V229 (≠ A219), A266 (= A253), A519 (≠ F469), H520 (= H470), D552 (= D503), I553 (≠ C504), S563 (≠ N514), P564 (≠ T515), M565 (≠ N516)
- binding p-nitrophenol: L93 (≠ V97), V361 (≠ I338), Y432 (≠ C384), L434 (= L386), G562 (= G513), S563 (≠ N514)
4h7uA Crystal structure of pyranose dehydrogenase from agaricus meleagris, wildtype (see paper)
28% identity, 95% coverage: 5:530/551 of query aligns to 16:572/577 of 4h7uA
- active site: A343 (≠ R314), V426 (≠ C384), Y510 (≠ I468), H512 (= H470), A555 (≠ G513), H556 (≠ N514)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-2,3,4-trihydroxy-5-[(4aR)-4a-hydroxy-7,8-dimethyl-2,4-dioxo-3,4,4a,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]pentyl dihydrogen diphosphate (non-preferred name): G22 (= G11), G24 (= G13), T25 (≠ S14), A26 (= A15), E46 (= E35), A47 (= A36), W74 (= W62), G99 (= G87), C100 (≠ S88), H103 (≠ I91), N104 (= N92), G105 (= G93), V107 (≠ I95), L242 (≠ M218), V243 (≠ A219), G282 (≠ A253), G283 (= G254), A286 (≠ Q257), H512 (= H470), A546 (≠ C504), H556 (≠ N514), T557 (= T515), Q558 (≠ N516), V561 (≠ I519)
6ze6A Fad-dependent oxidoreductase from chaetomium thermophilum in complex with fragment 4-nitrocatechol (see paper)
31% identity, 95% coverage: 5:530/551 of query aligns to 2:578/585 of 6ze6A
- binding 4-nitrocatechol: I75 (≠ P80), L92 (≠ V97), Q306 (≠ H294), V360 (≠ I338), Y431 (≠ C384), L433 (= L386), N514 (≠ G465), S516 (≠ T467), N517 (≠ I468), H519 (= H470), G561 (= G513), S562 (≠ N514)
- binding dihydroflavine-adenine dinucleotide: G8 (= G11), G10 (= G13), I11 (≠ S14), S12 (≠ A15), E32 (= E35), A33 (= A36), W56 (= W62), A77 (≠ G82), G82 (= G87), G83 (≠ S88), N87 (= N92), A88 (≠ G93), V90 (≠ I95), L227 (≠ M218), V228 (≠ A219), A265 (= A253), A518 (≠ F469), H519 (= H470), D551 (= D503), I552 (≠ C504), S562 (≠ N514), P563 (≠ T515), M564 (≠ N516)
6ze5A Fad-dependent oxidoreductase from chaetomium thermophilum in complex with fragment 2-(1h-indol-3-yl)-n-[(1-methyl-1h-pyrrol-2-yl) methyl]ethanamine (see paper)
31% identity, 95% coverage: 5:530/551 of query aligns to 2:578/585 of 6ze5A
- binding 2-(1H-indol-3-yl)-N-[(1-methyl-1H-pyrrol-2-yl)methyl]ethanamine: I75 (≠ P80), V90 (≠ I95), Y431 (≠ C384), N517 (≠ I468), D576 (= D528)
- binding dihydroflavine-adenine dinucleotide: G8 (= G11), G10 (= G13), I11 (≠ S14), S12 (≠ A15), E32 (= E35), A33 (= A36), W56 (= W62), A77 (≠ G82), G82 (= G87), G83 (≠ S88), N87 (= N92), A88 (≠ G93), V90 (≠ I95), L227 (≠ M218), V228 (≠ A219), A265 (= A253), A518 (≠ F469), H519 (= H470), D551 (= D503), I552 (≠ C504), S562 (≠ N514), P563 (≠ T515), M564 (≠ N516)
Sites not aligning to the query:
Query Sequence
>BPHYT_RS21745 FitnessBrowser__BFirm:BPHYT_RS21745
MADRFDFVVVGAGSAGCVLANRLSEGGRYSVCLLEAGPADRFMWIHIPIGYGKTMFHPVY
NWGFYTDPDPNMHNRRLYWPRGRTLGGSSSINGLIYVRGQRDDYDNWARLGNRGWSWQDC
LPYFKRLEHNELGEGPTRGVDGPLWASTIKQRHELVDAFIEASNSLGVASIDDFNTGDQE
GVGYYQLTTRRGFRCSTAVAYLKPARQRQNLHVETDAMASKILFEGTRACGVQYRQHGEL
REVRANREVILTAGALQSPQLLQLSGVGPAALLREFGIPVVANRAGVGENLQDHLQIRLI
YEVTKPITTNDQLRSWTGRAKMGLQWALMRSGPLAVGINQGGMFCRALPEESATPDTQFH
FSTLSADSAGGNVHDFPGCTYSICQLRPESRGAVRIRSADPREAPSIQPNYLDTDLDRRT
TIAGVRFARRVAAAQPMAGLMKREVRPGADAQTDDELLEFCREYGQTIFHPSGTAKMGPA
GDPLAVVDERLRVYGTQGLRVVDCSIMPTLVSGNTNVPIVMVAEKASDMILHDASGVDRG
VATTPAAKVAA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory