SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS21770 FitnessBrowser__BFirm:BPHYT_RS21770 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

8cekA Succinyl-coa reductase from clostridium kluyveri (sucd) with NADPH (see paper)
34% identity, 94% coverage: 23:473/481 of query aligns to 3:449/449 of 8cekA

query
sites
8cekA

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binding nadp nicotinamide-adenine-dinucleotide phosphate: P106 (= P128), I107 (≠ S129), H133 (≠ S155), P134 (= P156), T185 (= T208), G186 (= G209), G187 (≠ S210), R190 (≠ N213), V204 (= V227), C238 (= C261), E328 (= E352), L407 (≠ M432)

8cejC Succinyl-coa reductase from clostridium kluyveri (sucd) with mesaconyl-c1-coa (see paper)
34% identity, 94% coverage: 23:473/481 of query aligns to 3:449/449 of 8cejC

query
sites
8cejC

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binding Mesaconyl Coenzme A: K66 (= K88), P106 (= P128), T108 (= T130), N109 (= N131), A131 (= A153), P132 (= P154), H133 (≠ S155), P134 (= P156), R169 (≠ K192), G189 (≠ A212), R190 (≠ N213), I237 (≠ S260), C238 (= C261), S239 (= S262), T391 (≠ C415), G394 (= G419), T405 (≠ L430), L407 (≠ M432)

8cejA Succinyl-coa reductase from clostridium kluyveri (sucd) with mesaconyl-c1-coa (see paper)
34% identity, 94% coverage: 23:473/481 of query aligns to 3:449/449 of 8cejA

query
sites
8cejA

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D

E

A

E

A

A

A

V

E

E

V

I

V

A

R

M

S

A

I

N

Y

Y

A

M

Y

Y

M

E

G

G

A

A

G

G

H

H

S

T

V

A

G

G

L

I

H

H

S

S

S

D

D

N

A

D

A

E

Q

N

A

I

V

R

Q

Y

L

A

G

G

V

T

T

V

L

L

P

P

V

I

A

S

R

R

V

L

I

V

V

V

N

N

Q

Q

A

P

H

A

C

T

L

T

A

A

T

-

G
|
G

G

G

N

S

F

F

D

N

N

N

G

G

L

F

P

N

F

P

S

T

L
x
T

S

T

M
x
L

G

G

C

C

G

G

T

S

W

W

G

G

R

R

N

N

N

S

F

I

S

S

G

E

N

N

L

L

G

T

F

Y

R

E

H

H

Y

L

L

I

N

N

T

V

T

S

R

R

V

I

A

G

Y

Y

P

F

I

N

E

K

E

E

-

A

R

K

V

V

P

P

E

S

T

Y

D

E

E

E

L

I

L

W

G

G

binding (2e)-2-methylbut-2-enedioic acid: K66 (= K88), C238 (= C261), S239 (= S262), G394 (= G419), T405 (≠ L430), L407 (≠ M432)

5j7iC Crystal structure of a geobacillus thermoglucosidasius acetylating aldehyde dehydrogenase in complex with adp (see paper)
35% identity, 92% coverage: 23:463/481 of query aligns to 14:450/455 of 5j7iC

query
sites
5j7iC

V

V

A

R

T

N

L

Y

V

L

A

E

K

E

A

A

R

K

A

A

A

A

Q

Q

R

K

A

I

F

L

E

E

Q

K

A

M

G

T

Q

Q

Q

S

A

E

L

I

D

D

T

K

A

I

A

V

D

E

A

S

A

M

A

A

W

N

A

A

I

A

M

R

E

E

P

-

E

E

R

A

N

G

R

R

Q

-

L

L

A

A

E

A

R

M

A

A

V

V

H

E

D

E

T

T

G

G

L

F

G

G

N

N

A

V

D

E

D

D

K

K

F

T

R

L

K

K

N

N

Y

L

R

F

K

A

T

A

L

N

G

D

L

V

L

Y

R

N

D

S

L

I

H

K

E

D

Q

V

K

K

T

T

T

V

G

G

V

I

I

I

A

R

E

R

D

D

K

E

A

E

S

N

G

R

L

V

V

W

E

E

I

I

A

A

R

Q

A

P

V

V

G

G

V

I

V

V

A

A

A

G

I

I

T

I

P

P

S

S

T

T

N

N

P

P

A

T

A

S

T

T

P

V

A

I

N

F

K

K

I

A

I

L

N

I

A

A

L

V

K

K

C

A

G

R

N

N

A

A

V

I

I

V

V

F

A

S

P

P

S
x
H

P
|
P

K

S

G

A

Y

A

S

K

S

C

C

T

A

A

L

E

L

A

I

A

E

R

F

I

I

M

H

Q

A

E

Q

A

F

A

A

E

K

R

A

A

G

G

I

A

D

P

P

K

D

G

L

L

V

I

Q

S

M

C

L

I

P

T

A

Q

P

P

I

-

S

T

K

M

A

A

A

A

T

T

A

N

A

E

L

L

M

M

R

K

Q

H

-

K

-

L

C

T

D

D

L

V

V

I

V

L

A

A

T

T

G

G

S

G

Q

P

A

G

N
x
L

V

V

R

K

M

A

A

A

Y

Y

A

S

S

S

G

G

T

K

P

P

A

A

F

Y

G

G

V

V

G

G

A

P

G

G

N

N

V

V

A

P

S

V

I

Y

V

I

T

H

A

E

S

S

A

A

D

N

L

I

H

A

D

K

A

A

A

V

R

Q

K

L

I

I

L

I

R

Q

S

S

K

K

T

T

F

F

D

D

N

Y

A

G

T

T

S

I

C

C

S

A

S

S

E

E

N

Q

S

A

I

L

V

L

I

V

E

D

E

E

A

S

V

I

Y

K

Q

E

P

K

M

V

L

V

A

A

E

E

L

L

T

K

S

Q

C

Q

G

G

G

A

V

Y

L

F

L

L

D

N

A

E

A

E

Q

E

K

K

A

Q

K

K

L

V

Q

A

T

S

A

I

M

I

W

M

V

V

N

N

G

G

K

S

L

L

S

N

E

A

Q

K

C

I

T

V

A

G

K

K

S

A

A

P

E

Q

V

V

I

I

A

A

R

E

V

M

A

A

G

G

L

I

E

E

E

-

V

-

A

I

A

P

R

S

H

D

P

V

A

K

F

L

L

L

M

V

V

A

E

E

E

E

S

T

G

E

A

V

G

G

S

K

D

E

Y

Y

P

P

F

F

S

S

G

I

E

E

K

K

L

L

A

S

P

P

V

I

L

L

T

A

V

F

Y

Y

R

I

A

V

A

K

G

G

F

M

D

E

A

E

A

A

A

S

E

E

V

L

V

A

R

Q

S

K

I

L

Y

L

A

E

Y

V

M

G

G

G

A

L

G

G

H

H

S

T

V

V

G

G

L

I

H

H

S

A

S

E

D

D

A

E

A

K

Q

V

A

I

V

E

Q

A

L

Y

G

T

V

I

T

D

L

K

P

P

V

A

A

G

R

R

V

I

I

V

V

V

N

N

Q

A

A

G

H

T

C

T

L

F

A

G

T

G

G

I

G

G

N

A

F

T

D

V

N

N

G

V

L

K

P

P

F

-

S

S

L

L

S

T

M

L

G

G

C

C

G

G

T

A

W

I

G

G

R

N

N

N

N

I

F

T

S

S

G

D

N

N

L

V

G

T

F

V

R

T

H

H

Y

L

L

F

N

N

T

I

T

K

R

R

V

V

A

A

Y

F

P

G

I

V

E

R

E

E

binding adenosine-5'-diphosphate: H144 (≠ S155), P145 (= P156), L203 (≠ N213)

5j7iB Crystal structure of a geobacillus thermoglucosidasius acetylating aldehyde dehydrogenase in complex with adp (see paper)
35% identity, 92% coverage: 23:463/481 of query aligns to 15:451/456 of 5j7iB

query
sites
5j7iB

V

V

A

R

T

N

L

Y

V

L

A

E

K

E

A

A

R

K

A

A

A

A

Q

Q

R

K

A

I

F

L

E

E

Q

K

A

M

G

T

Q

Q

Q

S

A

E

L

I

D

D

T

K

A

I

A

V

D

E

A

S

A

M

A

A

W

N

A

A

I

A

M

R

E

E

P

-

E

E

R

A

N

G

R

R

Q

-

L

L

A

A

E

A

R

M

A

A

V

V

H

E

D

E

T

T

G

G

L

F

G

G

N

N

A

V

D

E

D

D

K

K

F

T

R

L

K

K

N

N

Y

L

R

F

K

A

T

A

L

N

G

D

L

V

L

Y

R

N

D

S

L

I

H

K

E

D

Q

V

K

K

T

T

T

V

G

G

V

I

I

I

A

R

E

R

D

D

K

E

A

E

S

N

G

R

L

V

V

W

E

E

I

I

A

A

R

Q

A

P

V

V

G

G

V

I

V

V

A

A

A

G

I

I

T

I

P

P

S

S

T

T

N

N

P

P

A

T

A

S

T

T

P

V

A

I

N

F

K

K

I

A

I

L

N

I

A

A

L

V

K

K

C

A

G

R

N

N

A

A

V

I

I

V

V

F

A

S

P

P

S

H

P

P

K

S

G

A

Y

A

S

K

S

C

C

T

A

A

L

E

L

A

I

A

E

R

F

I

I

M

H

Q

A

E

Q

A

F

A

A

E

K

R

A

A

G

G

I

A

D

P

P

K

D

G

L

L

V

I

Q

S

M

C

L

I

P

T

A

Q

P

P

I

-

S

T

K

M

A

A

A

A

T

T

A

N

A

E

L

L

M

M

R

K

Q

H

-

K

-

L

C

T

D

D

L

V

V

I

V

L

A

A

T

T

G

G

S

G

Q

P

A

G

N
x
L

V

V

R

K

M

A

A

A

Y

Y

A

S

S

S

G

G

T

K

P

P

A

A

F

Y

G

G

V

V

G

G

A

P

G

G

N

N

V

V

A

P

S

V

I

Y

V

I

T

H

A

E

S

S

A

A

D

N

L

I

H

A

D

K

A

A

A

V

R

Q

K

L

I

I

L

I

R

Q

S

S

K

K

T

T

F

F

D

D

N

Y

A

G

T

T

S

I

C

C

S

A

S

S

E

E

N

Q

S

A

I

L

V

L

I

V

E

D

E

E

A

S

V

I

Y

K

Q

E

P

K

M

V

L

V

A

A

E

E

L

L

T

K

S

Q

C

Q

G

G

G

A

V

Y

L

F

L

L

D

N

A

E

A

E

Q

E

K

K

A

Q

K

K

L

V

Q

A

T

S

A

I

M

I

W

M

V

V

N

N

G

G

K

S

L

L

S

N

E
x
A

Q

K

C

I

T

V

A

G

K

K

S

A

A

P

E

Q

V

V

I

I

A

A

R

E

V

M

A

A

G

G

L

I

E

E

E

-

V

-

A

I

A

P

R

S

H

D

P

V

A

K

F

L

L

L

M

V

V

A

E

E

E

E

S

T

G

E

A

V

G

G

S

K

D

E

Y

Y

P

P

F

F

S

S

G

I

E

E

K

K

L

L

A

S

P

P

V

I

L

L

T

A

V

F

Y

Y

R

I

A

V

A

K

G

G

F

M

D

E

A

E

A

A

A

S

E

E

V

L

V

A

R

Q

S

K

I

L

Y

L

A

E

Y

V

M

G

G

G

A

L

G

G

H

H

S

T

V

V

G

G

L

I

H

H

S

A

S

E

D

D

A

E

A

K

Q

V

A

I

V

E

Q

A

L

Y

G

T

V

I

T

D

L

K

P

P

V

A

A

G

R

R

V

I

I

V

V

V

N

N

Q

A

A

G

H

T

C

T

L

F

A

G

T

G

G

I

G

G

N

A

F

T

D

V

N

N

G

V

L

K

P

P

F

-

S

S

L

L

S

T

M

L

G

G

C

C

G

G

T

A

W

I

G

G

R

N

N

N

N

I

F

T

S

S

G

D

N

N

L

V

G

T

F

V

R

T

H

H

Y

L

L

F

N

N

T

I

T

K

R

R

V

V

A

A

Y

F

P

G

I

V

E

R

E

E

binding adenosine-5-diphosphoribose: L204 (≠ N213), A300 (≠ E309)

P0A9Q7 Bifunctional aldehyde-alcohol dehydrogenase AdhE; Alcohol dehydrogenase E; EC 1.2.1.10; EC 1.1.1.1 from Escherichia coli (strain K12) (see 8 papers)
33% identity, 90% coverage: 26:458/481 of query aligns to 12:445/891 of P0A9Q7

query
sites
P0A9Q7

L

L

V

V

A

E

K

R

A

V

R

K

A

K

A

A

Q

Q

R

R

A

E

F

Y

E

A

Q

S

A

F

G

T

Q

Q

Q

E

A

Q

L

V

D

D

T

K

A

I

A

F

D

R

A

A

A

A

A

A

W

L

A

A

I

A

M

A

E

D

P

-

E

-

R

A

N

R

R

I

Q

P

L

L

A

A

E

K

R

M

A

A

V

V

H

A

D

E

T

S

G

G

L

M

G

G

N

I

A

V

D

E

D

D

K

K

F

V

R

I

K

K

N

N

Y

H

R

F

K

A

T

S

L

E

G

Y

L

I

L

Y

R

N

D

A

L

Y

H

K

E

D

Q

E

K

K

T

T

T

C

G

G

V

V

I

L

A

S

E

E

D

D

K

D

A

T

S

F

G

G

L

T

V

I

E

T

I

I

A

A

R

E

A

P

V

I

G

G

V

I

V

I

A

C

A

G

I
|
I

T
x
V

P
|
P

S
x
T

T
|
T

N
|
N

P

P

A

T

A

S

T

T

P

A

A

I

N

F

K

K

I

S

I

L

N

I

A

S

L

L

K

K

C

T

G

R

N

N

A

A

V

I

I

I

V

F

A

S

P

P

S

H

P

P

K

R

G

A

Y

K

S

D

S

A

C

T

A

N

L

K

L

A

I

A

E

D

F

I

I

V

H

L

A

Q

Q

A

F

A

A

I

K

A

A

A

G

G

I

A

D

P

P

K

D

D

L

L

V

I

Q

G

M

W

L

I

P

D

A

Q

P

P

I

-

S

S

K

V

A

E

A

L

T

S

A

N

A

A

L

L

M

M

R

H

Q

H

C

P

D

D

-

I

-

N

L

L

V

I

V

L

A

A

T

T

G

G

S
x
G

Q

P

A

G

N

M

V

V

R

K

M

A

A

A

Y

Y

A

S

S

S

G

G

T

K

P

P

A

A

F

I

G

G

V

V

G
|
G

A

A

G

G

N

N

V

T

A

P

S

V

I

V

V

I

T

D

A

E

S

T

A

A

D

D

L

I

H

K

D

R

A

A

A

V

R

A

K

S

I

V

L

L

R

M

S

S

K

K

T

T

F

F

D

D

N

N

A

G

T

V

S

I

C

C

S

A

S

S

E

E

N

Q

S

S

I

V

V

V

I

V

E

V

E

D

A

S

V

V

Y

Y

Q

D

P

A

M

V

L

R

A

E

E

R

L

F

T
x
A

S

T

C

H

G

G

G

G

V

Y

L

L

L

L

D

Q

A

G

A

K

Q

E

K

L

A

K

K

A

L

V

Q

Q

T

D

A

V

M

I

W

L

V

K

N

N

G

G

K

A

L

L

S

N

E

A

Q

A

C

I

T

V

A

G

K

Q

S

P

A

A

E

Y

V

K

I

I

A

A

R

E

V

L

A

A

G

G

L

F

E

S

E

-

V

-

A

V

A

P

R

E

H

N

P

T

A

K

F

I

L

L

M

I

V

G

E

E

E

V

S

T

G

V

A

V

G

D

S

E

D

S

Y

E

P

P

F

F

S

A

G

H

E
|
E

K

K

L

L

A

S

P

P

V

T

L

L

T

A

V

M

Y

Y

R

R

A

A

A

K

G

D

F

F

D

E

A

D

A

A

A

V

E

E

V

K

V

A

R

E

S
x
K

I

L

Y

V

A

A

Y

M

M

G

G

G

A

I

G

G

H

H

S

T

V

S

G

C

L

L

H

Y

S

T

S

D

D

Q

A

D

A

N

Q

Q

A

P

V

A

Q

R

-

V

-

S

-

Y

L

F

G

G

V

Q

T

K

L

M

P

K

V

T

A

A

R

R

V

I

I

L

V

I

N

N

Q

T

A

P

H

A

C

S

L

Q

A

G

T

G

G

I

G

G

N

D

F

L

D

Y

N

N

-

F

G

K

L

L

P

A

F

P

S

S

L

L

S

T

M
x
L

G

G

C

C

G

G

T

S

W

W

G

G

R

G

N

N

N

S

F

I

S

S

G

E

N

N

L

V

G

G

F

P

R

K

H

H

Y

L

L

I

N

N

T

K

T

K

R

T

V

V

A

A

IVPTTN 110:115 (≠ ITPSTN 126:131) binding
G195 (≠ S210) binding
G213 (= G228) binding
A267 (≠ T282) mutation to T: Shows aerobic growth ability on ethanol. Shows 5-6 fold increase in acetaldehyde dehydrogenase activity, but does not affect ethanol dehydrogenase activity. Shows decreased thermal enzyme stability and increased sensitivity to MCO damage. Shows increased protein stability and resistance to MCO; when associated with K-568.
E335 (= E352) binding
K358 (≠ S375) modified: N6-acetyllysine
L419 (≠ M432) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
446:449 mutation Missing: Can form dimers, but does not assemble into long filaments. Strongly affects ALDH activity, but not ADH activity.
487 binding
519 binding
546:550 binding
568 E→K: Partially restores protein stability and resistance to MCO damage; when associated with T-267.
610 binding
619 binding
653 binding
657 binding
670 mutation F->A,E,V: Disrupts spirosome formation. Affects the forward activity of ALDH.
723 binding
737 binding

P0A9Q8 Bifunctional aldehyde-alcohol dehydrogenase AdhE; Alcohol dehydrogenase E; EC 1.2.1.10; EC 1.1.1.1 from Escherichia coli O157:H7 (see paper)
33% identity, 90% coverage: 26:458/481 of query aligns to 12:445/891 of P0A9Q8

query
sites
P0A9Q8

L

L

V

V

A

E

K

R

A

V

R

K

A

K

A

A

Q

Q

R

R

A

E

F

Y

E

A

Q

S

A

F

G

T

Q

Q

Q

E

A

Q

L

V

D

D

T

K

A

I

A

F

D

R

A

A

A

A

A

A

W

L

A

A

I

A

M

A

E

D

P

-

E

-

R

A

N

R

R

I

Q

P

L

L

A

A

E

K

R

M

A

A

V

V

H

A

D

E

T

S

G

G

L

M

G

G

N

I

A

V

D

E

D

D

K

K

F

V

R

I

K

K

N

N

Y

H

R

F

K

A

T

S

L

E

G

Y

L

I

L

Y

R

N

D

A

L

Y

H

K

E

D

Q

E

K

K

T

T

T

C

G

G

V

V

I

L

A

S

E

E

D

D

K

D

A

T

S

F

G

G

L

T

V

I

E

T

I

I

A

A

R

E

A

P

V

I

G

G

V

I

V

I

A

C

A

G

I

I

T

V

P

P

S

T

T

T

N

N

P

P

A

T

A

S

T

T

P

A

A

I

N

F

K

K

I

S

I

L

N

I

A

S

L

L

K

K

C

T

G

R

N

N

A

A

V

I

I

I

V

F

A

S

P

P

S

H

P

P

K

R

G

A

Y

K

S

D

S

A

C

T

A

N

L

K

L

A

I

A

E

D

F

I

I

V

H

L

A

Q

Q

A

F

A

A

I

K

A

A

A

G

G

I

A

D

P

P

K

D

D

L

L

V

I

Q

G

M

W

L

I

P

D

A

Q

P

P

I

-

S

S

K

V

A

E

A

L

T

S

A

N

A

A

L

L

M

M

R

H

Q

H

C

P

D

D

-

I

-

N

L

L

V

I

V

L

A

A

T

T

G

G

S

G

Q

P

A

G

N

M

V

V

R

K

M

A

A

A

Y

Y

A

S

S

S

G

G

T

K

P

P

A

A

F

I

G

G

V

V

G

G

A

A

G

G

N

N

V

T

A

P

S

V

I

V

V

I

T

D

A

E

S

T

A

A

D

D

L

I

H

K

D

R

A

A

A

V

R

A

K

S

I

V

L

L

R

M

S

S

K

K

T

T

F

F

D

D

N

N

A

G

T

V

S

I

C

C

S

A

S

S

E

E

N

Q

S

S

I

V

V

V

I

V

E

V

E

D

A

S

V

V

Y

Y

Q

D

P

A

M

V

L

R

A

E

E

R

L

F

T

A

S

T

C

H

G

G

G

G

V

Y

L

L

L

L

D

Q

A

G

A

K

Q

E

K

L

A

K

K

A

L

V

Q

Q

T

D

A

V

M

I

W

L

V

K

N

N

G

G

K

A

L

L

S

N

E

A

Q

A

C

I

T

V

A

G

K

Q

S

P

A

A

E

Y

V

K

I

I

A

A

R

E

V

L

A

A

G

G

L

F

E

S

E

-

V

-

A

V

A

P

R

E

H

N

P

T

A

K

F

I

L

L

M

I

V

G

E

E

E

V

S

T

G

V

A

V

G

D

S

E

D

S

Y

E

P

P

F

F

S

A

G

H

E

E

K

K

L

L

A

S

P

P

V

T

L

L

T

A

V

M

Y

Y

R

R

A

A

A

K

G

D

F

F

D

E

A

D

A

A

A

V

E

E

V

K

V

A

R

E

S

K

I

L

Y

V

A

A

Y

M

M

G

G

G

A

I

G

G

H

H

S

T

V

S

G

C

L

L

H

Y

S

T

S

D

D

Q

A

D

A

N

Q

Q

A

P

V

A

Q

R

-

V

-

S

-

Y

L

F

G

G

V

Q

T

K

L

M

P

K

V

T

A

A

R

R

V

I

I

L

V

I

N

N

Q

T

A

P

H

A

C

S

L

Q

A

G

T

G

G

I

G

G

N

D

F

L

D

Y

N

N

-

F

G

K

L

L

P

A

F

P

S

S

L

L

S

T

M

L

G

G

C

C

G

G

T

S

W

W

G

G

R

G

N

N

N

S

F

I

S

S

G

E

N

N

L

V

G

G

F

P

R

K

H

H

Y

L

L

I

N

N

T

K

T

K

R

T

V

V

A

A

Sites not aligning to the query:

487 binding
519 binding
546:550 binding
597:598 binding
638 binding
653 binding
657 binding
723 binding
737 binding

7bvpA Adhe spirosome in extended conformation (see paper)
33% identity, 90% coverage: 26:458/481 of query aligns to 12:445/869 of 7bvpA

query
sites
7bvpA

L

L

V

V

A

E

K

R

A

V

R

K

A

K

A

A

Q

Q

R

R

A

E

F

Y

E

A

Q

S

A

F

G

T

Q

Q

Q

E

A

Q

L

V

D

D

T

K

A

I

A

F

D

R

A

A

A

A

A

A

W

L

A

A

I

A

M

A

E

D

P

-

E

-

R

A

N

R

R

I

Q

P

L

L

A

A

E

K

R

M

A

A

V

V

H

A

D

E

T

S

G

G

L

M

G

G

N

I

A

V

D

E

D

D

K

K

F

V

R

I

K

K

N

N

Y

H

R

F

K

A

T

S

L

E

G

Y

L

I

L

Y

R

N

D

A

L

Y

H

K

E

D

Q

E

K

K

T

T

T

C

G

G

V

V

I

L

A

S

E

E

D

D

K

D

A

T

S

F

G

G

L

T

V

I

E

T

I

I

A

A

R

E

A

P

V

I

G

G

V

I

V

I

A

C

A

G

I

I

T

V

P
|
P

S
x
T

T

T

N

N

P

P

A

T

A

S

T

T

P

A

A

I

N

F

K

K

I

S

I

L

N

I

A

S

L

L

K

K

C

T

G

R

N

N

A

A

V

I

I

I

V

F

A

S

P

P

S
x
H

P

P

K

R

G

A

Y

K

S

D

S

A

C

T

A

N

L

K

L

A

I

A

E

D

F

I

I

V

H

L

A

Q

Q

A

F

A

A

I

K

A

A

A

G

G

I

A

D

P

P

K

D

D

L

L

V

I

Q

G

M

W

L

I

P

D

A

Q

P

P

I

-

S

S

K

V

A

E

A

L

T

S

A

N

A

A

L

L

M

M

R

H

Q

H

C

P

D

D

-

I

-

N

L

L

V

I

V

L

A

A

T

T

G
|
G

S
x
G

Q

P

A

G

N
x
M

V

V

R

K

M

A

A

A

Y

Y

A

S

S

S

G

G

T

K

P

P

A

A

F

I

G

G

V
|
V

G
|
G

A
|
A

G

G

N

N

V

T

A

P

S

V

I

V

V

I

T

D

A

E

S

T

A

A

D

D

L

I

H

K

D

R

A

A

A

V

R

A

K

S

I

V

L

L

R

M

S

S

K

K

T

T

F

F

D

D

N

N

A

G

T

V

S

I

C
|
C

S

A

S

S

E

E

N

Q

S

S

I

V

V

V

I

V

E

V

E

D

A

S

V

V

Y

Y

Q

D

P

A

M

V

L

R

A

E

E

R

L

F

T

A

S

T

C

H

G

G

G

G

V

Y

L

L

L

L

D

Q

A

G

A

K

Q

E

K

L

A

K

K

A

L

V

Q

Q

T

D

A

V

M

I

W

L

V

K

N

N

G

G

K

A

L

L

S

N

E

A

Q

A

C

I

T

V

A

G

K

Q

S

P

A

A

E

Y

V

K

I

I

A

A

R

E

V

L

A

A

G

G

L

F

E

S

E

-

V

-

A

V

A

P

R

E

H

N

P

T

A

K

F

I

L

L

M

I

V

G

E

E

E

V

S

T

G

V

A

V

G

D

S

E

D

S

Y

E

P

P

F

F

S

A

G

H

E
|
E

K

K

L
|
L

A

S

P

P

V

T

L

L

T

A

V

M

Y

Y

R

R

A

A

A

K

G

D

F

F

D

E

A

D

A

A

A

V

E

E

V

K

V

A

R

E

S

K

I

L

Y

V

A

A

Y

M

M

G

G

G

A

I

G

G

H
|
H

S

T

V

S

G

C

L

L

H

Y

S

T

S

D

D

Q

A

D

A

N

Q

Q

A

P

V

A

Q

R

-

V

-

S

-

Y

L

F

G

G

V

Q

T

K

L

M

P

K

V

T

A

A

R

R

V

I

I

L

V

I

N

N

Q

T

A

P

H

A

C

S

L

Q

A

G

T

G

G

I

G

G

N

D

F

L

D

Y

N

N

-

F

G

K

L

L

P

A

F

P

S

S

L

L

S
x
T

M
x
L

G

G

C

C

G

G

T

S

W

W

G

G

R

G

N

N

N

S

F

I

S

S

G

E

N

N

L

V

G

G

F

P

R

K

H

H

Y

L

L

I

N

N

T

K

T

K

R

T

V

V

A

A

binding nicotinamide-adenine-dinucleotide: P112 (= P128), T113 (≠ S129), H139 (≠ S155), G194 (= G209), G195 (≠ S210), M198 (≠ N213), V212 (= V227), G213 (= G228), A214 (= A229), C246 (= C261), E335 (= E352), L337 (= L354), H367 (= H384), T418 (≠ S431), L419 (≠ M432)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 487, 489, 519, 547, 550, 597, 598, 601, 610, 619, 646, 737
binding zinc ion: 653, 657, 723, 737

6tqmA Escherichia coli adhe structure in its compact conformation (see paper)
33% identity, 90% coverage: 26:458/481 of query aligns to 12:445/869 of 6tqmA

query
sites
6tqmA

L

L

V

V

A

E

K

R

A

V

R

K

A

K

A

A

Q

Q

R

R

A

E

F

Y

E

A

Q

S

A

F

G

T

Q

Q

Q

E

A

Q

L

V

D

D

T

K

A

I

A

F

D

R

A

A

A

A

A

A

W

L

A

A

I

A

M

A

E

D

P

-

E

-

R

A

N

R

R

I

Q

P

L

L

A

A

E

K

R

M

A

A

V

V

H

A

D

E

T

S

G

G

L

M

G

G

N

I

A

V

D

E

D

D

K

K

F

V

R

I

K

K

N

N

Y

H

R

F

K

A

T

S

L

E

G

Y

L

I

L

Y

R

N

D

A

L

Y

H

K

E

D

Q

E

K

K

T

T

T

C

G

G

V

V

I

L

A

S

E

E

D

D

K

D

A

T

S

F

G

G

L

T

V

I

E

T

I

I

A

A

R

E

A

P

V

I

G

G

V

I

V

I

A

C

A

G

I

I

T

V

P

P

S

T

T

T

N

N

P

P

A

T

A

S

T

T

P

A

A

I

N

F

K

K

I

S

I

L

N

I

A

S

L

L

K

K

C

T

G

R

N

N

A

A

V

I

I

I

V

F

A

S

P

P

S

H

P

P

K

R

G

A

Y

K

S

D

S

A

C

T

A

N

L

K

L

A

I

A

E

D

F

I

I

V

H

L

A

Q

Q

A

F

A

A

I

K

A

A

A

G

G

I

A

D

P

P

K

D

D

L

L

V

I

Q

G

M

W

L

I

P

D

A

Q

P

P

I

-

S

S

K

V

A

E

A

L

T

S

A

N

A

A

L

L

M

M

R

H

Q

H

C

P

D

D

-

I

-

N

L

L

V

I

V

L

A

A

T

T

G

G

S

G

Q

P

A

G

N

M

V

V

R

K

M

A

A

A

Y

Y

A

S

S

S

G

G

T

K

P

P

A

A

F

I

G

G

V

V

G

G

A

A

G

G

N

N

V

T

A

P

S

V

I

V

V

I

T

D

A

E

S

T

A

A

D

D

L

I

H

K

D

R

A

A

A

V

R

A

K

S

I

V

L

L

R

M

S

S

K

K

T

T

F

F

D

D

N

N

A

G

T

V

S

I

C

C

S

A

S

S

E

E

N

Q

S

S

I

V

V

V

I

V

E

V

E

D

A

S

V

V

Y

Y

Q

D

P

A

M

V

L

R

A

E

E

R

L

F

T

A

S

T

C

H

G

G

G

G

V

Y

L

L

L

L

D

Q

A

G

A

K

Q

E

K

L

A

K

K

A

L

V

Q

Q

T

D

A

V

M

I

W

L

V

K

N

N

G

G

K

A

L

L

S

N

E

A

Q

A

C

I

T

V

A

G

K

Q

S

P

A

A

E

Y

V

K

I

I

A

A

R

E

V

L

A

A

G

G

L

F

E

S

E

-

V

-

A

V

A

P

R

E

H

N

P

T

A

K

F

I

L

L

M

I

V

G

E

E

E

V

S

T

G

V

A

V

G

D

S

E

D

S

Y

E

P

P

F

F

S

A

G

H

E

E

K

K

L

L

A

S

P

P

V

T

L

L

T

A

V

M

Y

Y

R

R

A

A

A

K

G

D

F

F

D

E

A

D

A

A

A

V

E

E

V

K

V

A

R

E

S

K

I

L

Y

V

A

A

Y

M

M

G

G

G

A

I

G

G

H

H

S

T

V

S

G

C

L

L

H

Y

S

T

S

D

D

Q

A

D

A

N

Q

Q

A

P

V

A

Q

R

-

V

-

S

-

Y

L

F

G

G

V

Q

T

K

L

M

P

K

V

T

A

A

R

R

V

I

I

L

V

I

N

N

Q

T

A

P

H

A

C

S

L

Q

A

G

T

G

G

I

G

G

N

D

F

L

D

Y

N

N

-

F

G

K

L

L

P

A

F

P

S

S

L

L

S

T

M

L

G

G

C

C

G

G

T

S

W

W

G

G

R

G

N

N

N

S

F

I

S

S

G

E

N

N

L

V

G

G

F

P

R

K

H

H

Y

L

L

I

N

N

T

K

T

K

R

T

V

V

A

A

Sites not aligning to the query:

Q9XDN1 Propanal dehydrogenase (CoA-propanoylating); Coenzyme-A-acylating propionaldehyde dehydrogenase; Propanediol utilization protein PduP; EC 1.2.1.87 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
29% identity, 79% coverage: 18:395/481 of query aligns to 5:400/464 of Q9XDN1

query
sites
Q9XDN1

E
|
E

A
x
L

E
|
E

R
x
T

I
x
L

V
x
I

A
x
R

T
|
T

L
x
I

V
x
L

A
x
S

K
x
E

-
x
Q

-
x
L

A

T

R

T

A

P

A

A

Q

Q

R

T

A

P

F

V

E

Q

Q

P

A

Q

G

G

Q

K

-

G

-

I

-

F

Q

Q

A

S

L

V

D

S

T

E

A

A

A

I

D

D

A

A

A

A

A

H

W

Q

A

A

I

F

M

L

E

R

-

Y

-

Q

-

Q

-

C

P

P

E

L

R

K

N

T

R

R

Q

S

-

A

-

I

-

I

-

S

-

A

-

M

-

R

-

Q

-

E

-

L

-

T

-

P

-

L

-

L

-

A

-

P

L

L

A

A

E

E

R

E

A

S

V

A

H

N

D

E

T

T

G

G

L

M

G

G

N

N

A

K

D

E

D

D

K

K

F

F

R

L

K

K

N

N

-

K

-

A

-

A

Y

L

R

D

K

N

T

T

L

P

G

G

L

V

L

-

R

-

D

-

L

-

H

-

E

E

Q

D

K

L

T

T

T

T

G

T

V

A

I

L

A

T

E

G

D

D

K

G

A

G

S

M

G

V

L

L

V

F

E

E

I

Y

A

S

R

-

A

P

V

F

G

G

V

V

V

I

A

G

A

S

I

V

T

A

P

P

S

S

T

T

N

N

P

P

A

T

A

E

T

T

P

I

A

I

N

N

K

N

I

S

I

I

N

S

A

M

L

L

K

A

C

A

G

G

N

N

A

S

V

I

I

Y

V

F

A

S

P

P

S

H

P

P

K

G

G

A

Y

K

S

K

S

V

C

S

A

L

L

K

L

L

I

I

E

S

F

L

I

I

H

E

A

E

-

I

Q

A

F

F

A

R

K

C

A

C

G

G

I

I

D

R

P

-

D

N

L

L

V

V

Q

V

M

T

L

V

P

A

A

E

P

P

I

T

S

F

K

E

A

A

A

-

T

T

A

Q

A

Q

L

M

M

M

R

A

Q

H

C

P

D

R

L

I

V

A

V

V

-

L

-

A

A

I

T

T

G

G

S

G

Q

P

A

G

N

I

V

V

R

A

M

M

A

G

Y

M

A

K

S

S

G

G

T

K

P

K

A

V

F

I

G

G

V

A

G

G

A

A

G

G

N

N

V

P

A

P

S

C

I

I

V

V

T

D

A

E

S

T

A

A

D

D

L

L

H

V

D

K

A

A

A

A

R

E

K

D

I

I

L

I

R

N

S

G

K

A

T

S

F

F

D

D

N

Y

A

N

T

L

S

P

C

C

S

I

S

A

E

E

N

K

S

S

I

L

V

I

I

V

E

V

E

E

A

S

V

V

Y

A

Q

E

P

R

M

L

L

V

A

Q

E

Q

L

M

T

Q

S

T

C

F

G

G

G

A

V

L

L

L

L

L

D

S

A

P

A

A

Q

D

K

T

A

D

K

K

L

L

Q

R

T

A

A

V

M

C

W

L

V

P

N

E

G

G

K

Q

L

A

S

N

E

K

Q

K

C

L

T

V

A

G

K

K

S

S

A

P

E

S

V

A

I

M

A

L

R

E

V

A

A

A

G

G

L

I

E

-

E

A

V

V

A

P

A

A

R

K

H

A

P

P

A

R

F

L

L

L

M

I

V

-

E

-

E

-

S

A

G

L

A

V

G

N

S

A

D

D

Y

D

P

P

F

W

-

V

S

T

G

S

E

E

K

Q

L

L

A

M

P

P

V

M

L

L

T

P

V

V

Y

V

R

K

A

V

A

S

G

D

F

F

D

D

A

S

A

A

A

-

E

-

V

L

V

A

R

L

S

A

I

L

Y

K

A

V

Y

E

M

E

G

G

A

L

G

H

H

H

S

T

V

A

G

I

L

M

H

H

S

S

S

Q

D

N

A

V

A

S

Q

R

E7 (= E20) mutation to A: Substantially decreased protein levels in BMCs, no change in enzyme activity.
I10 (≠ V23) mutation to A: Substantially decreased protein levels in BMCs, no change in enzyme activity.
L14 (≠ V27) mutation to A: Substantially decreased protein levels in BMCs, no change in enzyme activity.

Sites not aligning to the query:

1:10 mutation Missing: Much less protein associates with BMCs, no effect on enzyme activity.
1:14 mutation Missing: Much less protein associates with BMCs, no effect on enzyme activity.
1:18 Targets protein to the BMC
2:18 mutation Missing: No longer interacts with PduA.

6gvsA Engineered glycolyl-coa reductase comprising 8 mutations with bound NADP+ (see paper)
28% identity, 72% coverage: 56:399/481 of query aligns to 44:381/441 of 6gvsA

query
sites
6gvsA

I

I

M

L

E

N

P

Q

E

D

R

T

N

L

R

E

Q

K

L

M

A

S

E

R

R

M

A

A

V

V

H

E

D

E

T

T

G

G

L

M

G

G

N

N

A

Y

D

E

D

H

K

K

F

L

R

I

K

K

N

N

Y

R

-

L

-

A

-

G

R

E

K

K

T

T

L

P

G

G

L

I

L

-

R

-

D

-

L

-

H

-

E

E

Q

D

K

L

T

T

T

T

G

D

V

A

I

F

A

S

E

G

D

D

K

N

A

G

S

L

G

T

L

L

V

V

E

E

I

Y

A

S

R

-

A

P

V

F

G

G

V

V

V

I

A

G

A

A

I

I

T

T

P
|
P

S
x
T

T

T

N

N

P

P

A

T

A

E

T

T

P

I

A

V

N

C

K

N

I

S

I

I

N

G

A

M

L

L

K

A

C

A

G

G

N

N

A

S

V

V

I

V

V

F

A

S

P

P

S
x
H

P

G

K

R

G

A

Y

R

S

Q

S

V

C

S

A

L

L

L

L

L

I

V

E

R

F

L

I

I

H

N

A

Q

Q

K

F

L

A

A

K

A

A

L

G

G

I

A

D

P

P

E

D

N

L

L

V

V

Q

V

M

T

L

V

P

E

A

K

P

P

I

-

S

S

K
x
R

A

E

A

N

T

T

A

L

A

A

L

M

M

M

-

A

-

H

R

P

Q

K

C

V

D

R

L

M

V

L

V

V

A

A

T
|
T

G
|
G

S
x
G

Q

P

A

A

N
x
L

V

V

R

K

M

A

A

V

Y

L

A

S

S

T

G

G

T

K

P

K

A

A

F

I

G

G

V
x
A

G
|
G

A
|
A

G

G

N

N

V

P

A