SitesBLAST
Comparing BPHYT_RS22690 FitnessBrowser__BFirm:BPHYT_RS22690 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 13 hits to proteins with known functional sites (download)
P0AA76 D-galactonate transporter; D-galactonate/H(+) symporter from Escherichia coli (strain K12) (see paper)
45% identity, 95% coverage: 18:454/458 of query aligns to 3:429/430 of P0AA76
- Y29 (= Y44) binding
- D31 (= D46) mutation to N: Loss of galactonate transport activity.
- R32 (= R47) binding
- Y64 (= Y79) binding
- E118 (= E133) mutation to Q: Loss of galactonate transport activity.
- W358 (= W382) binding
6e9nA E. Coli d-galactonate:proton symporter in the inward open form (see paper)
46% identity, 93% coverage: 27:453/458 of query aligns to 1:409/409 of 6e9nA
6e9oA E. Coli d-galactonate:proton symporter mutant e133q in the outward substrate-bound form (see paper)
44% identity, 94% coverage: 24:453/458 of query aligns to 1:393/393 of 6e9oA
Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
26% identity, 85% coverage: 29:417/458 of query aligns to 70:465/582 of Q9JI12
- R88 (= R47) mutation to A: Impairs synaptic transmission. Abolishes the chloride ion conductance.
- H128 (≠ F72) mutation to A: Greatly lowers L-glutamate transport.
- R184 (= R126) mutation R->A,E,K: Greatly lowers L-glutamate transport.
- E191 (= E133) mutation to A: Greatly lowers L-glutamate transport.; mutation E->D,Q: Lowers L-glutamate transport.
- R322 (≠ G276) mutation to A: Loss of L-glutamate release. Abolishes the chloride ion conductance.
7t3nA R184q/e191q mutant of rat vesicular glutamate transporter 2 (vglut2)
25% identity, 85% coverage: 29:417/458 of query aligns to 12:407/452 of 7t3nA
- binding (1s,3r)-1-aminocyclopentane-1,3-dicarboxylic acid: Y77 (= Y79), Y137 (≠ F137), Y165 (= Y165), R264 (≠ G276), F268 (≠ L280), Y269 (≠ V281)
- binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: R12 (= R29), Y13 (= Y30), E152 (= E152), G163 (= G163)
Q03567 Uncharacterized transporter slc-17.2 from Caenorhabditis elegans (see paper)
25% identity, 86% coverage: 60:452/458 of query aligns to 75:466/493 of Q03567
Sites not aligning to the query:
- 69 modified: carbohydrate, N-linked (GlcNAc...) asparagine
Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
23% identity, 88% coverage: 56:458/458 of query aligns to 96:489/495 of Q9NRA2
- K136 (= K96) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
- H183 (≠ S141) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
- LL 198:199 (≠ AT 156:157) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
- IL 266:267 (≠ IE 223:224) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
- SSLRN 268:272 (≠ SAGGA 238:242) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
- G328 (= G303) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
- P334 (= P309) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
- G371 (≠ A344) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172
Sites not aligning to the query:
- 22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
- 39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794
Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
25% identity, 87% coverage: 59:458/458 of query aligns to 99:489/495 of Q5Q0U0
- K136 (= K96) mutation to E: Markedly decreases H(+)-coupled sialic acid transporter activity.
- R168 (= R126) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
- E171 (≠ L129) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
- G172 (= G130) mutation to C: Decreases protein levels and alters subcellular localization.
- E175 (= E133) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
- G176 (≠ A134) mutation to C: Decreases protein levels and alters subcellular localization.
- F179 (= F137) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
- P180 (= P138) mutation to C: Decreases protein levels and alters subcellular localization.
- H183 (≠ S141) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
- W186 (≠ V144) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
- SSLKN 268:272 (≠ THRKK 230:234) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
- P334 (= P309) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
- G371 (≠ A344) mutation to V: Remains in the endoplasmic reticulum.
Sites not aligning to the query:
- 22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
- 39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.
Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
24% identity, 87% coverage: 59:458/458 of query aligns to 99:489/495 of Q8BN82
- H183 (≠ S141) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.
Sites not aligning to the query:
- 39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.
Q9Y2C5 Probable small intestine urate exporter; Solute carrier family 17 member 4 from Homo sapiens (Human) (see 2 papers)
24% identity, 84% coverage: 64:449/458 of query aligns to 106:481/497 of Q9Y2C5
- A372 (≠ I343) to T: in dbSNP:rs11754288
Sites not aligning to the query:
- 66 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
- 75 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-66 and A-90.
- 90 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
Q9GQQ0 Protein spinster; Protein benchwarmer; Protein diphthong from Drosophila melanogaster (Fruit fly) (see paper)
21% identity, 79% coverage: 27:388/458 of query aligns to 111:449/605 of Q9GQQ0
- E217 (= E133) mutation to K: In bnch(N); leads to storage in yolk spheres during oogenesis and results in widespread accumulation of enlarged lysosomal and late endosomal inclusions.
Q51955 4-hydroxybenzoate transporter PcaK from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
23% identity, 37% coverage: 26:193/458 of query aligns to 24:190/448 of Q51955
- D41 (≠ N43) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
- D44 (= D46) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
- G85 (= G87) mutation to V: Abolishes 4-HBA transport and chemotaxis.
- D89 (= D91) mutation to N: Abolishes 4-HBA transport and chemotaxis.
- G92 (= G94) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to C: No change in 4-HBA transport and chemotaxis.; mutation G->L,V: Abolishes 4-HBA transport and chemotaxis.; mutation to Q: Decrease in 4-HBA transport and strong decrease in chemotaxis.
- R124 (= R126) mutation to A: Abolishes 4-HBA transport.
- E144 (≠ T146) mutation to A: Strong decrease in 4-HBA transport.
- H183 (≠ R185) mutation to A: Decrease in 4-HBA transport and chemotaxis.
Sites not aligning to the query:
- 323 D→N: Abolishes 4-HBA transport and chemotaxis.
- 328 H→A: Decrease in 4-HBA transport and chemotaxis.; H→R: Decrease in 4-HBA transport and loss of chemotaxis.
- 386 R→A: Strong decrease in 4-HBA transport.
- 398 R→A: Abolishes 4-HBA transport.
- 444 H→A: No change in 4-HBA transport and chemotaxis.
Q9UPI3 Heme transporter FLVCR2; Calcium-chelate transporter; CCT; Feline leukemia virus subgroup C receptor-related protein 2 from Homo sapiens (Human) (see 2 papers)
20% identity, 99% coverage: 1:453/458 of query aligns to 58:493/526 of Q9UPI3
Sites not aligning to the query:
- 16 V → A: in dbSNP:rs2287015
- 30:66 HPSVSVHPSVSINPSVSVHPSSSAHPSALAQPSGLAH→APSVSVAPSVSINPSVSVAPSSSAAPSALAQPSGLAA: Loss of heme-binding activity.
Query Sequence
>BPHYT_RS22690 FitnessBrowser__BFirm:BPHYT_RS22690
MQQPIQSSSADAATPQGVTASTARRSKARYQILALLAVGTMINYLDRTVLGIAAPQLTKE
LGINAALMGLLFSVFSWSYVASQIPGGLFLDRFGSKLTYFLSMTLWSLCTLAQGLVHGIG
ALFAFRLGLGVSEAPCFPTNSRVVATWFPQSERAMATGTYTVGEYIGLAFFSPFLFMLMG
AFGWRSLFYVVGGVGIVFGGIWWMFYREPRDHPSANQAELDYIEAGGGLTHRKKDADSAG
GADAASAKSGGFEWRTIGRLLKHRQLTGICLGQFAGNSTLVFFLTWFPTYLATERHMAWL
KIGFFAIMPFIAASIGVMFGGFFSDWLLRRGKSPNVARKLPIIAGLLLASTIILANYVES
NVAVIAILSVAFFAQGMAALGWTLVSDIAPDGLLGVTGGIFNFAANLAGIVTPLVVGFIV
AATGSFVGALVFIGAIALIGALSYIFIVGDIKRIVLVD
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory