SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS23135 FitnessBrowser__BFirm:BPHYT_RS23135 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

P68183 Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12) (see 2 papers)
29% identity, 66% coverage: 63:261/303 of query aligns to 86:285/296 of P68183

query
sites
P68183

W

W

L

N

S

S

L

V

K

K

I

V

G

A

L

G

L

I

T

S

A

A

C

I

A

G

S

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V

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C

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Y

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A

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M

G

-

R

R

F

F

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P

G

G

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F

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T

T

L

L

F

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K

G

G

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M

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P

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L

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L

L

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L

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G

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P

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G

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E
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E

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A
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G
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L

E190 (= E169) mutation E->A,C,K,L: Reduction of transport rate.
A192 (= A171) mutation A->D,S,L: Loss of transport and MalK dissociation from the membrane.
G196 (= G175) mutation to A: No effect.; mutation to P: Loss of transport and MalK dissociation from the membrane.
P209 (= P188) mutation to A: No effect.

4ki0F Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to maltohexaose (see paper)
34% identity, 27% coverage: 141:223/303 of query aligns to 363:440/490 of 4ki0F

query
sites
4ki0F

W

W

I

L

G
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G

H

Y

V

-

M

-

L

-

C

-

V

-

S

P

Y
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Y

V

M

A

M

I

I

I

L

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C

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M

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G

R

L

V

L

K

K

E

A

L

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N

P

R

D

S

D

L

L

E

Y

E

E

A

A

A

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L

A

D

M

L

D

G

G

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A

T

G

P

P

F

F

K

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N

F

F

F

F

L

K

I

I

T

T

L

L

P

P

L

L

I

L

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K

A

P

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G

L

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S

F

F

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x
N

F

F

D

N

D

N

L

F

V

V

L

L

S

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A

Q

F

L

L

L

S

T

G

N

P

G

G

G

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P

T

D

T

R

L

L

binding alpha-D-glucopyranose: G365 (= G143), Y368 (= Y151), S418 (= S201), N422 (≠ S205)

Sites not aligning to the query:

binding alpha-D-glucopyranose: 310, 361

P02916 Maltose/maltodextrin transport system permease protein MalF from Escherichia coli (strain K12) (see 4 papers)
34% identity, 27% coverage: 141:223/303 of query aligns to 378:455/514 of P02916

query
sites
P02916

W

W

I

L

G
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G

H

Y

V

-

M

-

L

-

C

-

V

-

S

P

Y

Y

V

M

A

M

I

I

I

L

V

C

Q

M

S

G

R

L

V

L

K

K

E

A

L

I

N

P

R

D

S

D

L

L

E

Y

E
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E

A

A

A
x
S

L

A

D

M

L

D

G
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G

A

A

T

G

P

P

F

F

K

Q

V

N

F

F

F

F

L

K

I

I

T

T

L

L

P
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P

L

L

I

L

S

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K

A

P

L

L

M

T

S

P

G

L

W

M

L

I

L

A

S

S

F

F

T

A

L

F

S

N

F

F

D

N

D

N

L

F

V

V

L

L

S

I

A

Q

F

L

L

L

S

T

G

N

P

G

G

G

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P

T

D

T

R

L

L

G380 (= G143) mutation G->C,S: No growth on maltose.
E401 (= E169) mutation E->A,C,K,L: Reduction of transport rate.
S403 (≠ A171) mutation S->C,D,K,L: Reduction of transport rate.
G407 (= G175) mutation G->A,P: No effect.
P420 (= P188) mutation to A: No effect.

Sites not aligning to the query:

334 L→W: Ability to transport lactose in a saturable manner.
372 L→W: Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose.
376 mutation N->K,H: No growth on maltose.

Query Sequence

>BPHYT_RS23135 FitnessBrowser__BFirm:BPHYT_RS23135
MIKPNKTLSNSVLTFGFLFLYIPIISLIVYSFNESKLVTVWSGFSLKWYGALLQDGELLN
AAWLSLKIGLLTACASVVIGTWAGFVLARFGRFRGFTLFAGMINAPLVIPEVIQGISLLL
LFVALEQMLGWPKGRGLFTIWIGHVMLCVSYVAIIVQSRVKELNRSLEEAALDLGATPFK
VFFLITLPLISQALMSGWLLSFTLSFDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
ALATIFITTVTIGVIAVNRWMQLRERKRNRDMQMAFALAEAADPLPAAPQQAAARKSLDT
ARA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory