SitesBLAST
Comparing BPHYT_RS24035 FitnessBrowser__BFirm:BPHYT_RS24035 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3c4jA Abc protein artp in complex with atp-gamma-s
55% identity, 87% coverage: 24:273/289 of query aligns to 3:242/242 of 3c4jA
3c41J Abc protein artp in complex with amp-pnp/mg2+
55% identity, 87% coverage: 24:273/289 of query aligns to 3:242/242 of 3c41J
2olkA Abc protein artp in complex with adp-beta-s
55% identity, 87% coverage: 24:273/289 of query aligns to 3:242/242 of 2olkA
2oljA Abc protein artp in complex with adp/mg2+
55% identity, 87% coverage: 24:273/289 of query aligns to 3:242/242 of 2oljA
4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
54% identity, 85% coverage: 24:269/289 of query aligns to 1:236/240 of 4ymuJ
- binding adenosine-5'-triphosphate: F11 (= F34), V16 (= V39), S36 (= S59), G37 (= G60), S38 (= S61), G39 (= G62), K40 (= K63), S41 (= S64), T42 (= T65), E162 (= E195), H194 (= H227)
- binding magnesium ion: S41 (= S64), E162 (= E195)
4u00A Crystal structure of ttha1159 in complex with adp (see paper)
55% identity, 87% coverage: 23:273/289 of query aligns to 1:240/241 of 4u00A
1b0uA Atp-binding subunit of the histidine permease from salmonella typhimurium (see paper)
51% identity, 87% coverage: 25:275/289 of query aligns to 3:255/258 of 1b0uA
P02915 Histidine/lysine/arginine/ornithine transport ATP-binding protein HisP; EC 7.4.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
52% identity, 85% coverage: 25:270/289 of query aligns to 7:254/258 of P02915
- S41 (= S59) binding
- G42 (= G60) binding
- G44 (= G62) binding
- K45 (= K63) binding
- S46 (= S64) binding
- T47 (= T65) binding
P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
37% identity, 91% coverage: 24:287/289 of query aligns to 1:254/343 of P30750
- 40:46 (vs. 59:65, 86% identical) binding
- E166 (= E195) mutation to Q: Exhibits little ATPase activity.
Sites not aligning to the query:
- 278:283 binding
- 295 N→A: Reduces the binding of L-methionine to undetectable levels.
- 295:296 binding
3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
36% identity, 91% coverage: 24:287/289 of query aligns to 2:255/344 of 3tuzC
Sites not aligning to the query:
3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
36% identity, 91% coverage: 24:287/289 of query aligns to 2:255/344 of 3tuiC
6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
36% identity, 91% coverage: 24:287/289 of query aligns to 2:255/344 of 6cvlD
- binding phosphothiophosphoric acid-adenylate ester: F12 (= F34), Q14 (≠ E36), I19 (≠ V37), S41 (= S59), G42 (= G60), A43 (≠ S61), G44 (= G62), K45 (= K63), S46 (= S64), T47 (= T65), N141 (≠ K169), S143 (= S171), Q146 (= Q174), H200 (= H227)
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
40% identity, 81% coverage: 25:258/289 of query aligns to 3:222/374 of 2awnB
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
40% identity, 81% coverage: 25:258/289 of query aligns to 3:222/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F34), S37 (= S59), G38 (= G60), C39 (≠ S61), G40 (= G62), K41 (= K63), S42 (= S64), T43 (= T65), Q81 (= Q117), R128 (≠ A165), A132 (≠ K169), S134 (= S171), G136 (= G173), Q137 (= Q174), E158 (= E195), H191 (= H227)
- binding magnesium ion: S42 (= S64), Q81 (= Q117)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
40% identity, 81% coverage: 25:258/289 of query aligns to 3:222/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ F34), G38 (= G60), C39 (≠ S61), G40 (= G62), K41 (= K63), S42 (= S64), T43 (= T65), R128 (≠ A165), S134 (= S171), Q137 (= Q174)
- binding beryllium trifluoride ion: S37 (= S59), G38 (= G60), K41 (= K63), Q81 (= Q117), S134 (= S171), G136 (= G173), H191 (= H227)
- binding magnesium ion: S42 (= S64), Q81 (= Q117)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
40% identity, 81% coverage: 25:258/289 of query aligns to 3:222/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ F34), V17 (= V39), G38 (= G60), C39 (≠ S61), G40 (= G62), K41 (= K63), S42 (= S64), T43 (= T65), R128 (≠ A165), A132 (≠ K169), S134 (= S171), Q137 (= Q174)
- binding tetrafluoroaluminate ion: S37 (= S59), G38 (= G60), K41 (= K63), Q81 (= Q117), S134 (= S171), G135 (= G172), G136 (= G173), E158 (= E195), H191 (= H227)
- binding magnesium ion: S42 (= S64), Q81 (= Q117)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
40% identity, 81% coverage: 25:258/289 of query aligns to 3:222/371 of 3puvA
- binding adenosine-5'-diphosphate: W12 (≠ F34), V17 (= V39), G38 (= G60), C39 (≠ S61), G40 (= G62), K41 (= K63), S42 (= S64), T43 (= T65), R128 (≠ A165), A132 (≠ K169), S134 (= S171), Q137 (= Q174)
- binding magnesium ion: S42 (= S64), Q81 (= Q117)
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
40% identity, 81% coverage: 25:258/289 of query aligns to 4:223/371 of P68187
- A85 (≠ N120) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ S142) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (≠ A150) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ M153) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (= E155) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (= A160) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G173) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D194) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
Sites not aligning to the query:
- 228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 267 W→G: Normal maltose transport but constitutive mal gene expression.
- 278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
40% identity, 81% coverage: 25:258/289 of query aligns to 1:220/367 of 1q12A
- binding adenosine-5'-triphosphate: W10 (≠ F34), S35 (= S59), G36 (= G60), C37 (≠ S61), G38 (= G62), K39 (= K63), S40 (= S64), T41 (= T65), R126 (≠ A165), A130 (≠ K169), S132 (= S171), G134 (= G173), Q135 (= Q174)
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
39% identity, 81% coverage: 25:258/289 of query aligns to 4:223/369 of P19566
- L86 (= L121) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P196) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (= D201) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
Sites not aligning to the query:
- 306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
Query Sequence
>BPHYT_RS24035 FitnessBrowser__BFirm:BPHYT_RS24035
MNAIADMPSSASSATVGSSSGAPLIEVRDLRKRFGEVEVLRGVDLEIARSEVVCIIGPSG
SGKSTLLRCLAALETYDQGDVRIEGELLGYSERNGKRVRASQSEINRVRRNVGMVFQQFN
LWPHMTALGNVMESLLRVRHLSRDEARRRANAMLETVGLAHKGDAYPAKLSGGQQQRVAI
ARALAMEPHIMLFDEPTSALDPELVGEVLQVMKQLARDGMTMAVVTHEMGFAAQVADKVM
FIDQGRIAVQGKPRDVFHDAGQPRLRQFLQNYFDRNAFWTRGPDDAQPL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory