SitesBLAST

Q7CRQ0 Uronate dehydrogenase; D-galacturonate dehydrogenase; D-glucuronate dehydrogenase; Hexuronate dehydrogenase; EC 1.1.1.203 from Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)) (see paper)
46% identity, 92% coverage: 1:256/278 of query aligns to 1:257/265 of Q7CRQ0

query
sites
Q7CRQ0

M

M

K

K

K

R

I

L

A

L

L

V

S

T

G

G

A

A

G

A

G

G

Q

Q

L

L

G

G

S

R

V

V

V

M

R

R

A

E

A

R

L

L

I

A

A

P

R

M

G

A

T

E

P

I

L

L

R

R

S

L

A

A

A

D

G

L

S

S

K

P

A

-

L

L

V

D

P

P

L

A

V

G

D

P

G

N

E

E

D

E

V

C

M

V

H

Q

G

C

D

D

L

L

R

A

D

D

P

A

A

N

V

A

V

V

D

N

R

A

L

M

L

V

E

A

G

G

V

C

D

D

V

G

L

I

I

V

H

H

F

L

A

G

G

G

T

I

S

S

V

V

E

E

R

K

P

P

L

F

P

E

E

Q

I

I

I

L

E

Q

N

G

N

N

L

I

R

I

G

G

L

L

V

Y

E

N

V

L

Y

Y

E

E

G

A

A

A

R

R

R

A

Q

H

G

G

V

Q

K

P

R

R

I

I

V

V

F

F

A

A

S

S

N

N

H

H

A

T

I

I

G

G

M

Y

Y

Y

P

P

V

Q

T

T

E

E

H

R

L

L

S

G

L

P

D

D

C

V

E

P

L

A

R

R

P

P

D

D

G

G

F

L

Y
|
Y

G

G

L

V

S

S

K

K

V

C

W

F

G

G

E

E

A

N

L

L

A

A

R

R

M

M

Y

Y

W

F

D

D

K

K

H

F

G

G

I

Q

E

E

S

T

V

A

C

L

V

V

R

R

I

I

G

G

S

S

C

C

L

T

E

P

R

E

P

P

T

N

E

N

P

Y

R

R

H

M

L

L

S

S

T

T

W

W

F

F

G

S

H

H

R

D

D

D

L

F

L

V

H

S

F

L

L

I

D

E

R

A

C

V

V

F

E

R

A

A

E

P

N

V

V

L

G

G

F

C

L

P

T

V

I

V

W

W

G

G

V

A

S

S

A

A

N

N

T

D

R

A

S

G

W

W

D

D

N

N

S

S

G

H

A

L

E

G

R

F

L

L

G

G

Y

W

Q

K

P

P

T

K

Q

D

D

N

A

A

E

E

V

A

Y

F

-

R

-

H

A

I

E

T

E

E

I

T

L

T

A

P

R

P

P

P

N

D

P

P

L

N

D

D

A

A

L

L

G

-

Q

V

R

R

F

F

Q

Q

G

G

S

T

F

F

V

V

Y134 (= Y135) mutation to A: 0.1% of wild-type activity.

3rfxA Crystal structure of uronate dehydrogenase from agrobacterium tumefaciens, y136a mutant complexed with NAD (see paper)
46% identity, 92% coverage: 1:256/278 of query aligns to 2:258/265 of 3rfxA

query
sites
3rfxA

M

M

K

K

K

R

I

L

A

L

L

V

S

T

G
|
G

A

A

G

A

G
|
G

Q
|
Q

L
|
L

G

G

S

R

V

V

V

M

R

R

A

E

A

R

L

L

I

A

A

P

R

M

G

A

T

E

P

I

L

L

R

R

S

L

A

A

A
x
D

G
x
L

S

S

K

P

A

-

L

L

V

D

P

P

L

A

V

G

D

P

G

N

E

E

D

E

V

C

M

V

H

Q

G

C

D
|
D

L
|
L

R

A

D

D

P

A

A

N

V

A

V

V

D

N

R

A

L

M

L

V

E

A

G

G

V

C

D

D

V

G

L

I

I

V

H

H

F

L

A

G

G
|
G

T

I

S
|
S

V

V

E

E

R

K

P

P

L

F

P

E

E

Q

I

I

I

L

E

Q

N

G

N

N

L

I

R

I

G

G

L

L

V

Y

E

N

V

L

Y

Y

E

E

G

A

A

A

R

R

R

A

Q

H

G

G

V

Q

K

P

R

R

I

I

V

V

F

F

A

A

S

S

N

N

H

H

A

T

I

I

G

G

M

Y

Y

Y

P

P

V

Q

T

T

E

E

H

R

L

L

S

G

L

P

D

D

C

V

E

P

L

A

R

R

P

P

D

D

G

G

F

L

Y

A

G

G

L

V

S

S

K

K

V

C

W

F

G

G

E

E

A

N

L

L

A

A

R

R

M

M

Y

Y

W

F

D

D

K

K

H

F

G

G

I

Q

E

E

S

T

V

A

C

L

V

V

R

R

I

I

G

G

S

S

C

C

L

T

E

P

R

E

P

P

T

N

E

N

P

Y

R

R

H

M

L

L

S

S

T

T

W

W

F

F

G

S

H

H

R

D

D

D

L

F

L

V

H

S

F

L

L

I

D

E

R

A

C

V

V

F

E

R

A

A

E

P

N

V

V

L

G

G

F

C

L

P

T

V

I

V

W

W

G

G

V

A

S

S

A

A

N

N

T

D

R

A

S

G

W

W

D

D

N

N

S

S

G

H

A

L

E

G

R

F

L

L

G

G

Y

W

Q

K

P

P

T

K

Q

D

D

N

A

A

E

E

V

A

Y

F

-

R

-

H

A

I

E

T

E

E

I

T

L

T

A

P

R

P

P

P

N

D

P

P

L

N

D

D

A

A

L

L

G

-

Q

V

R

R

F

F

Q

Q

G

G

S

T

F

F

V

V

binding nicotinamide-adenine-dinucleotide: G9 (= G8), G12 (= G11), Q13 (= Q12), L14 (= L13), D33 (≠ A32), L34 (≠ G33), D50 (= D50), L51 (= L51), G72 (= G72), S74 (= S74)

1r6dA Crystal structure of desiv double mutant (dtdp-glucose 4,6- dehydratase) from streptomyces venezuelae with NAD and dau bound (see paper)
29% identity, 76% coverage: 3:212/278 of query aligns to 2:217/322 of 1r6dA

query
sites
1r6dA

K

R

I

L

A

L

L

V

S

T

G

G

A

G

G

A

G
|
G

Q
x
F

L
x
I

G

G

S

S

V

H

V

F

R

V

A

R

A

Q

L

L

I

L

A

A

R

G

G

A

T

Y

P

P

L

D

R

V

S

P

A

A

-

D

-

E

-

V

-

I

-

V

-

L

-
x
D

-
x
S

-
x
L

-
x
T

-

Y

A
|
A

G
|
G

S

N

K

R

A

A

L

N

V

L

P

A

L

P

V

V

D

D

G

A

E

D

D

P

-

R

-

L

-

R

V

F

M

V

H

H

G

G

D
|
D

L
x
I

R

R

D

D

P

A

A

G

V

L

D

A

R

R

L

E

L

L

E

R

G

G

V

V

D

D

V

A

L

I

I

V

H

H

F
|
F

A
|
A

G
x
A

T

E

S
|
S

-
x
H

V

V

E

D

R

R

P

S

L

I

-

A

-

G

-

A

P

S

E

V

I

F

I

T

E

E

N
x
T

N

N

L

V

R

Q

G

G

L

T

V

Q

E

T

V

L

Y

L

E

Q

G

C

A

A

R

V

R

D

Q

A

G

G

V

V

K

G

R

R

I

V

V

V

F

H

A
x
V

S
|
S

S
x
T

N
|
N

H
x
Q

A

V

I

Y

G

G

M

S

Y

I

P

D

V

-

T

S

E

G

H

S

L

W

S

T

L

E

D

S

C

S

E

P

L

L

R

E

P

P

D

N

G

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F

P

Y
|
Y

G

A

L

A

S

S

K
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K

V

A

W

G

G

S

E

D

A

L

L

V

A

A

R

R

M

A

Y

Y

W

H

D

R

K

T

H

Y

G

G

I

L

E

D

S

-

V

-

C

-

V

V

R

R

I

I

G

T

S

R

C

C

L

C

E
x
N

R
x
N

-

Y

-

G

P

P

T

Y

E

Q

-

H

P

P

R

E

H
x
K

L
|
L

S

I

T

P

W

L

F

F

G

-

H

-

R

-

D

-

L

V

L

T

H

N

F

L

L

L

D

D

R

G

C

-

V

-

E

-

A

-

E

-

N

-

V

-

G

G

F

T

L

L

T
x
P

I

L

W
x
Y

G

G

V

D

S

G

A

A

N

N

T

V

R
|
R

S

E

W

W

active site: T127 (≠ S110), N128 (= N111), Q129 (≠ H112), Y151 (= Y135), K155 (= K139)
binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: S87 (= S74), H88 (vs. gap), T127 (≠ S110), N128 (= N111), Q129 (≠ H112), Y151 (= Y135), N180 (≠ E167), K190 (≠ H174), L191 (= L175), P206 (≠ T201), Y208 (≠ W203), R215 (= R210)
binding nicotinamide-adenine-dinucleotide: G10 (= G11), F11 (≠ Q12), I12 (≠ L13), D37 (vs. gap), S38 (vs. gap), L39 (vs. gap), T40 (vs. gap), A42 (= A32), G43 (= G33), D63 (= D50), I64 (≠ L51), F83 (= F70), A84 (= A71), A85 (≠ G72), S87 (= S74), T102 (≠ N85), V125 (≠ A108), S126 (= S109), Y151 (= Y135), K155 (= K139), N181 (≠ R168)

Sites not aligning to the query:

binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: 250, 274, 277, 281

1r66A Crystal structure of desiv (dtdp-glucose 4,6-dehydratase) from streptomyces venezuelae with NAD and tyd bound (see paper)
28% identity, 76% coverage: 3:212/278 of query aligns to 2:217/322 of 1r66A

query
sites
1r66A

K

R

I

L

A

L

L

V

S

T

G

G

A

G

G

A

G
|
G

Q
x
F

L
x
I

G

G

S

S

V

H

V

F

R

V

A

R

A

Q

L

L

I

L

A

A

R

G

G

A

T

Y

P

P

L

D

R

V

S

P

A

A

-

D

-

E

-

V

-

I

-

V

-

L

-
x
D

-
x
S

-
x
L

-
x
T

-

Y

A

A

G
|
G

S

N

K

R

A

A

L

N

V

L

P

A

L

P

V

V

D

D

G

A

E

D

D

P

-

R

-

L

-

R

V

F

M

V

H

H

G

G

D
|
D

L
x
I

R

R

D

D

P

A

A

G

V

L

D

A

R

R

L

E

L

L

E

R

G

G

V

V

D

D

V

A

L

I

I

V

H

H

F
|
F

A
|
A

G
x
A

T

E

S
|
S

-
x
H

V

V

E

D

R

R

P

S

L

I

-

A

-

G

-

A

P

S

E

V

I

F

I

T

E

E

N
x
T

N

N

L

V

R

Q

G

G

L

T

V

Q

E

T

V

L

Y

L

E

Q

G

C

A

A

R

V

R

D

Q

A

G

G

V

V

K

G

R

R

I

V

V

V

F

H

A
x
V

S
|
S

S
x
T

N
x
D

H
x
E

A

V

I

Y

G

G

M

S

Y

I

P

D

V

-

T

S

E

G

H

S

L

W

S

T

L

E

D

S

C

S

E

P

L

L

R

E

P

P

D

N

G

S

F

P

Y
|
Y

G

A

L

A

S

S

K
|
K

V

A

W

G

G

S

E

D

A

L

L

V

A

A

R

R

M

A

Y

Y

W

H

D

R

K

T

H

Y

G

G

I

L

E

D

S

-

V

-

C

-

V

V

R

R

I

I

G

T

S

R

C

C

L

C

E
x
N

R
x
N

-

Y

-

G

P

P

T

Y

E

Q

-

H

P

P

R

E

H
x
K

L
|
L

S

I

T

P

W

L

F

F

G

-

H

-

R

-

D

-

L

V

L

T

H

N

F

L

L

L

D

D

R

G

C

-

V

-

E

-

A

-

E

-

N

-

V

-

G

G

F

T

L

L

T
x
P

I

L

W
x
Y

G

G

V

D

S

G

A

A

N

N

T

V

R
|
R

S

E

W

W

active site: T127 (≠ S110), D128 (≠ N111), E129 (≠ H112), Y151 (= Y135), K155 (= K139)
binding nicotinamide-adenine-dinucleotide: G10 (= G11), F11 (≠ Q12), I12 (≠ L13), D37 (vs. gap), S38 (vs. gap), L39 (vs. gap), T40 (vs. gap), G43 (= G33), D63 (= D50), I64 (≠ L51), F83 (= F70), A84 (= A71), A85 (≠ G72), S87 (= S74), T102 (≠ N85), V125 (≠ A108), S126 (= S109), Y151 (= Y135), K155 (= K139), N181 (≠ R168)
binding thymidine-5'-diphosphate: H88 (vs. gap), E129 (≠ H112), N180 (≠ E167), K190 (≠ H174), L191 (= L175), P206 (≠ T201), Y208 (≠ W203), R215 (= R210)

Sites not aligning to the query:

binding thymidine-5'-diphosphate: 250, 274, 277

7cgvA Full consensus l-threonine 3-dehydrogenase, fctdh-iiym (NAD+ bound form) (see paper)
28% identity, 62% coverage: 2:172/278 of query aligns to 1:176/310 of 7cgvA

query
sites
7cgvA

K

K

K

R

I

I

A

L

L

V

S

I

G
|
G

A

A

G

N

G
|
G

Q
|
Q

L
x
I

G

G

S

S

V

E

V

L

R

V

A

P

A

A

L

L

I

R

A

K

R

R

G

Y

T

G

P

A

L

D

R

N

S

V

A

I

A

A

G

S

S
x
D

K
x
I

A
x
R

L

P

V

P

P

N

L

L

V

Y

D

E

G

A

E

G

D

P

V

F

M

E

H

Y

G
x
L

D
|
D

L
x
V

R

L

D

D

P

K

A

E

V

A

V

L

D

A

R

E

L

L

L

V

E

K

G

K

V

Y

D

K

V

I

-

T

-

Q

L

I

I

Y

H

H

F

L

A
|
A

G
x
A

-

L

-
x
L

-

S

T

A

S

T

V

G

E

E

R

K

P

N

L

P

P

Q

E

K

I

A

I

W

E

D

N

L

N

N

L

M

R

D

G

G

L

L

V

L

E

N

V

V

Y

L

E

E

G

I

A

A

R

R

R

E

Q

R

G

G

V

P

K

L

R

K

I

V

V

F

F

W

A
x
P

S

S

S
|
S

N

I

H

A

A

A

I

F

G

G

M

P

Y

N

P

T

V

P

T

K

E

D

H

N

L

T

S

P

L

Q

D

D

C

T

E

I

L

M

R

R

P

P

D

T

G

T

F

I

Y
|
Y

G

G

L

I

S

S

K
|
K

V

V

W

A

G

G

E

E

A

L

L

L

A

C

R

E

M

Y

Y

Y

W

H

D

T

K

K

H

Y

G

G

I

V

E

D

S

V

V

R

C

S

V

V

R

R

I
x
Y

G

P

S

G

C
x
I

L

I

E

S

R

Y

P

K

T

T

E

P

P

P

binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), Q11 (= Q12), I12 (≠ L13), D33 (≠ S34), I34 (≠ K35), R35 (≠ A36), L48 (≠ G49), D49 (= D50), V50 (≠ L51), A72 (= A71), A73 (≠ G72), L75 (vs. gap), P112 (≠ A108), S114 (= S110), Y139 (= Y135), K143 (= K139), Y166 (≠ I162), I169 (≠ C165)

4id9B Crystal structure of a short-chain dehydrogenase/reductase superfamily protein from agrobacterium tumefaciens (target efi-506441) with bound NAD, monoclinic form 1
30% identity, 57% coverage: 4:162/278 of query aligns to 3:160/328 of 4id9B

query
sites
4id9B

I

I

A

L

L

V

S

T

G

G

A

S

G

A

G
|
G

Q
x
R

L
x
V

G

G

S

R

V

A

V

V

R

V

A

A

L

L

I

R

A

T

R

Q

G

G

T

-

P

-

L

-

R

R

S

T

A

V

A

R

G

G

S

F

K
x
D

A
x
L

L

R

V

P

P

S

L

G

V

T

D

G

G

G

E

E

D

E

V

V

M

V

H

-

G

G

D
x
S

L
|
L

R

E

D

D

P

G

A

Q

V

A

V

L

D

S

R

D

L

A

L

I

E

M

G

G

V

V

D

S

V

A

L

V

I

L

H

H

F
x
L

A

G

G
x
A

T

F

S

M

V

S

E

W

R

A

P

P

L

A

P

D

E

R

-

D

-

R

I

M

I

F

E

A

N
x
V

N

N

L

V

R

E

G

G

L

T

V

R

E

R

V

L

Y

L

E

D

G

A

A

A

R

S

R

A

Q

A

G

G

V

V

K

R

R

R

I

F

V

V

F

F

A

A

S

S

S
|
S

N

G

H

E

A

V

I

Y

-

P

-

E

-

N

-

R

-

P

G

E

M

F

Y

L

P

P

V

V

T

T

E

E

H

-

L

-

S

-

L

-

D

D

C

H

E

P

L

L

R

C

P

P

D

N

G

S

F

P

Y
|
Y

G

G

L

L

S

T

K
|
K

V

L

W

L

G

G

E

E

A

E

L

L

A

V

R

R

M

F

Y

H

W

Q

D

R

K

S

H

G

G

A

I

M

E

E

S

T

V

V

C

I

V

L

R

R

I
x
F

active site: S107 (= S110), Y133 (= Y135), K137 (= K139)
binding nicotinamide-adenine-dinucleotide: G10 (= G11), R11 (≠ Q12), V12 (≠ L13), D31 (≠ K35), L32 (≠ A36), S45 (≠ D50), L46 (= L51), L65 (≠ F70), A67 (≠ G72), V82 (≠ N85), Y133 (= Y135), K137 (= K139), F160 (≠ I162)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 161, 162, 163

4id9A Crystal structure of a short-chain dehydrogenase/reductase superfamily protein from agrobacterium tumefaciens (target efi-506441) with bound NAD, monoclinic form 1
30% identity, 57% coverage: 4:162/278 of query aligns to 2:159/321 of 4id9A

query
sites
4id9A

I

I

A

L

L

V

S

T

G

G

A

S

G

A

G
|
G

Q
x
R

L
x
V

G

G

S

R

V

A

V

V

R

V

A

A

L

L

I

R

A

T

R

Q

G

G

T

-

P

-

L

-

R

R

S

T

A

V

A

R

G

G

S

F

K
x
D

A
x
L

L
x
R

V

P

P

S

L

G

V

T

D

G

G

G

E

E

D

E

V

V

M

V

H

-

G

G

D
x
S

L
|
L

R

E

D

D

P

G

A

Q

V

A

V

L

D

S

R

D

L

A

L

I

E

M

G

G

V

V

D

S

V

A

L

V

I

L

H

H

F
x
L

A

G

G
x
A

T

F

S

M

V

S

E

W

R

A

P

P

L

A

P

D

E

R

-

D

-

R

I

M

I

F

E

A

N
x
V

N

N

L

V

R

E

G

G

L

T

V

R

E

R

V

L

Y

L

E

D

G

A

A

A

R

S

R

A

Q

A

G

G

V

V

K

R

R

R

I

F

V

V

F

F

A

A

S

S

S
|
S

N

G

H

E

A

V

I

Y

G

P

-

E

-

N

-

R

-

P

-

E

M

F

Y

L

P

P

V

V

T

T

E

E

H

-

L

-

S

-

L

-

D

D

C

H

E

P

L

L

R

C

P

P

D

N

G

S

F

P

Y
|
Y

G

G

L

L

S

T

K
|
K

V

L

W

L

G

G

E

E

A

E

L

L

A

V

R

R

M

F

Y

H

W

Q

D

R

K

S

H

G

G

A

I

M

E

E

S

T

V

V

C

I

V

L

R

R

I
x
F

active site: S106 (= S110), Y132 (= Y135), K136 (= K139)
binding nicotinamide-adenine-dinucleotide: G9 (= G11), R10 (≠ Q12), V11 (≠ L13), D30 (≠ K35), L31 (≠ A36), R32 (≠ L37), S44 (≠ D50), L45 (= L51), L64 (≠ F70), A66 (≠ G72), V81 (≠ N85), S106 (= S110), Y132 (= Y135), K136 (= K139), F159 (≠ I162)

Sites not aligning to the query:

2udpA Udp-galactose 4-epimerase complexed with udp-phenol (see paper)
27% identity, 51% coverage: 3:145/278 of query aligns to 2:159/338 of 2udpA

query
sites
2udpA

K

R

I

V

A

L

L

V

S

T

G
|
G

A

G

G

S

G
|
G

Q
x
Y

L
x
I

G

G

S

S

V

H

V

T

R

C

A

V

A

Q

L

L

I

L

A

Q

R

N

G

G

T

H

P

D

-

V

-

I

L

L

R
x
D

S
x
N

A
x
L

A
x
C

G
x
N

S
|
S

K

K

-

R

A

S

L

V

V

L

P

P

L

V

V

I

D

E

-

R

-

L

-

G

-

K

G

H

E

P

D

T

V

F

M

V

H

E

G

G

D
|
D

L
x
I

R

R

D

N

P

E

A

A

V

L

V

M

D

T

R

E

L

I

L

L

E

H

-

D

-

H

G

A

V

I

D

D

V

T

L

V

I

I

H

H

F
|
F

A
|
A

G
|
G

-

L

-
x
K

-

A

-

V

-

G

T

E

S

S

V

V

E

Q

R

K

P

P

L

L

P

-

E

E

I

Y

E

D

N

N

L

V

R

N

G

G

L

T

V

L

E

R

V

L

Y

I

E

S

G

A

A

M

R

R

R

A

Q

A

G

N

V

V

K

K

R

N

I

F

V

I

F

F

A
x
S

S

S

S
|
S

N
x
A

H
x
T

A

V

I

Y

G

G

M

D

Y

Q

P

P

V

K

T

I

E

P

H

Y

L

V

S

E

L

S

D

F

C

P

E

T

L

G

R

T

P

P

D

Q

G

S

F

P

Y
|
Y

G

G

L

K

S

S

K
|
K

V

L

W

M

G

V

E

E

A

Q

L

I

active site: S124 (= S110), A125 (≠ N111), T126 (≠ H112), Y149 (= Y135), K153 (= K139)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), Y11 (≠ Q12), I12 (≠ L13), D31 (≠ R29), N32 (≠ S30), L33 (≠ A31), C34 (≠ A32), N35 (≠ G33), S36 (= S34), D58 (= D50), I59 (≠ L51), F80 (= F70), A81 (= A71), G82 (= G72), K84 (vs. gap), S122 (≠ A108), S124 (= S110), Y149 (= Y135), K153 (= K139)

Sites not aligning to the query:

active site: 189
binding nicotinamide-adenine-dinucleotide: 177
binding phenyl-uridine-5'-diphosphate: 179, 199, 200, 216, 217, 218, 231, 233, 269, 292, 295

1udcA Structure of udp-galactose-4-epimerase complexed with udp-mannose (see paper)
27% identity, 51% coverage: 3:145/278 of query aligns to 2:159/338 of 1udcA

query
sites
1udcA

K

R

I

V

A

L

L

V

S

T

G
|
G

A

G

G

S

G
|
G

Q
x
Y

L
x
I

G

G

S

S

V

H

V

T

R

C

A

V

A

Q

L

L

I

L

A

Q

R

N

G

G

T

H

P

D

-

V

-

I

L

L

R
x
D

S
x
N

A

L

A
x
C

G
x
N

S
|
S

K

K

-

R

A

S

L

V

V

L

P

P

L

V

V

I

D

E

-

R

-

L

-

G

-

K

G

H

E

P

D

T

V

F

M

V

H

E

G

G

D
|
D

L
x
I

R

R

D

N

P

E

A

A

V

L

V

M

D

T

R

E

L

I

L

L

E

H

-

D

-

H

G

A

V

I

D

D

V

T

L

V

I

I

H

H

F
|
F

A
|
A

G
|
G

-

L

-
x
K

-

A

-

V

-

G

T

E

S

S

V

V

E

Q

R

K

P

P

L

L

P

-

E

E

I

Y

E

D

N

N

L

V

R

N

G

G

L

T

V

L

E

R

V

L

Y

I

E

S

G

A

A

M

R

R

R

A

Q

A

G

N

V

V

K

K

R

N

I

F

V

I

F

F

A
x
S

S

S

S
|
S

N
x
A

H
x
T

A

V

I

Y

G

G

M

D

Y

Q

P

P

V

K

T

I

E

P

H

Y

L

V

S

E

L

S

D

F

C

P

E

T

L

G

R

T

P

P

D

Q

G

S

F

P

Y
|
Y

G

G

L

K

S

S

K
|
K

V

L

W

M

G

V

E

E

A

Q

L

I

active site: S124 (= S110), A125 (≠ N111), T126 (≠ H112), Y149 (= Y135), K153 (= K139)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), Y11 (≠ Q12), I12 (≠ L13), D31 (≠ R29), N32 (≠ S30), C34 (≠ A32), N35 (≠ G33), S36 (= S34), D58 (= D50), I59 (≠ L51), F80 (= F70), A81 (= A71), G82 (= G72), K84 (vs. gap), S122 (≠ A108), Y149 (= Y135), K153 (= K139)
binding uridine-5'-diphosphate-mannose: T126 (≠ H112), Y149 (= Y135)

Sites not aligning to the query:

active site: 189
binding nicotinamide-adenine-dinucleotide: 177, 180
binding uridine-5'-diphosphate-mannose: 179, 199, 200, 215, 216, 217, 218, 231, 233, 269, 292

P09147 UDP-glucose 4-epimerase; Galactowaldenase; UDP-galactose 4-epimerase; EC 5.1.3.2 from Escherichia coli (strain K12) (see 10 papers)
27% identity, 51% coverage: 3:145/278 of query aligns to 2:159/338 of P09147

query
sites
P09147

K

R

I

V

A

L

L

V

S

T

G

G

A

G

G

S

G

G

Q
x
Y

L
x
I

G

G

S

S

V

H

V

T

R

C

A

V

A

Q

L

L

I

L

A

Q

R

N

G

G

T

H

P

D

-

V

-

I

L

L

R
x
D

S
x
N

A
x
L

A
x
C

G
x
N

S
|
S

K

K

-

R

A

S

L

V

V

L

P

P

L

V

V

I

D

E

-

R

-

L

-

G

-

K

G

H

E

P

D

T

V

F

M

V

H

E

G

G

D
|
D

L
x
I

R

R

D

N

P

E

A

A

V

L

V

M

D

T

R

E

L

I

L

L

E

H

-

D

-

H

G

A

V

I

D

D

V

T

L

V

I

I

H

H

F
|
F

A
|
A

G
|
G

-
x
L

-
x
K

-

A

-

V

-

G

T

E

S

S

V

V

E

Q

R

K

P

P

L

L

P

-

E

E

I

Y

E

D

N
|
N

N

N

L

V

R

N

G

G

L

T

V

L

E

R

V

L

Y

I

E

S

G

A

A

M

R

R

R

A

Q

A

G

N

V

V

K

K

R

N

I

F

V

I

F

F

A

S

S

S

S
|
S

N

A

H

T

A

V

I

Y

G

G

M

D

Y

Q

P

P

V

K

T

I

E

P

H

Y

L

V

S

E

L

S

D

F

C

P

E

T

L

G

R

T

P

P

D

Q

G

S

F

P

Y
|
Y

G

G

L

K

S

S

K
|
K

V

L

W

M

G

V

E

E

A

Q

L

I

YI 11:12 (≠ QL 12:13) binding
DNLCNS 31:36 (≠ RSAAGS 29:34) binding
DI 58:59 (≠ DL 50:51) binding
FAGLK 80:84 (≠ FAG-- 70:72) binding
N99 (= N85) binding
S124 (= S110) binding ; mutation to A: No major structural changes. Catalytic efficiency is very low and affinity binding is 21% of the wild-type enzyme.; mutation to T: No major structural changes. Catalytic efficiency is about 30% of that of the wild-type enzyme, and affinity binding is similar to that of the native enzyme.
Y149 (= Y135) active site, Proton acceptor; binding ; mutation to F: No major structural changes. Catalytic efficiency is very low and affinity binding is 12% of the wild-type enzyme.
K153 (= K139) binding ; mutation to A: Decreases the catalytic activity. Not reduced by sugars in the presence or absence of UMP. It contains very little NADH.; mutation to M: Decreases the catalytic activity. Not reduced by sugars in the presence or absence of UMP. It contains very little NADH.

Sites not aligning to the query:

178 binding
299 Y→C: Loss of epimerase activity with UDP-Gal by almost 5-fold, but it results in a gain of epimerase activity with uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) by 230-fold with minimal changes in its three-dimensional structure.

1udaA Structure of udp-galactose-4-epimerase complexed with udp-4-deoxy-4- fluoro-alpha-d-galactose (see paper)
27% identity, 51% coverage: 3:145/278 of query aligns to 2:159/338 of 1udaA

query
sites
1udaA

K

R

I

V

A

L

L

V

S

T

G
|
G

A

G

G

S

G
|
G

Q
x
Y

L
x
I

G

G

S

S

V

H

V

T

R

C

A

V

A

Q

L

L

I

L

A

Q

R

N

G

G

T

H

P

D

-

V

-

I

L

L

R
x
D

S
x
N

A
x
L

A
x
C

G
x
N

S
|
S

K

K

-

R

A

S

L

V

V

L

P

P

L

V

V

I

D

E

-

R

-

L

-

G

-

K

G

H

E

P

D

T

V

F

M

V

H

E

G

G

D
|
D

L
x
I

R

R

D

N

P

E

A

A

V

L

V

M

D

T

R

E

L

I

L

L

E

H

-

D

-

H

G

A

V

I

D

D

V

T

L

V

I

I

H

H

F
|
F

A
|
A

G
|
G

-

L

-
x
K

-

A

-

V

-

G

T

E

S

S

V

V

E

Q

R

K

P

P

L

L

P

-

E

E

I

Y

E

D

N

N

L

V

R

N

G

G

L

T

V

L

E

R

V

L

Y

I

E

S

G

A

A

M

R

R

R

A

Q

A

G

N

V

V

K

K

R

N

I

F

V

I

F

F

A
x
S

S

S

S
|
S

N
x
A

H
x
T

A

V

I

Y

G

G

M

D

Y

N

P

P

V

K

T

I

E

P

H

Y

L

V

S

E

L

S

D

F

C

P

E

T

L

G

R

T

P

P

D

Q

G

S

F

P

Y
|
Y

G

G

L

K

S

S

K
|
K

V

L

W

M

G

V

E

E

A

Q

L

I

active site: S124 (= S110), A125 (≠ N111), T126 (≠ H112), Y149 (= Y135), K153 (= K139)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), Y11 (≠ Q12), I12 (≠ L13), D31 (≠ R29), N32 (≠ S30), L33 (≠ A31), C34 (≠ A32), N35 (≠ G33), S36 (= S34), D58 (= D50), I59 (≠ L51), F80 (= F70), A81 (= A71), G82 (= G72), K84 (vs. gap), S122 (≠ A108), Y149 (= Y135), K153 (= K139)
binding uridine-5'-diphosphate-4-deoxy-4-fluoro-alpha-d-galactose: T126 (≠ H112)

Sites not aligning to the query:

active site: 189
binding nicotinamide-adenine-dinucleotide: 177, 180
binding uridine-5'-diphosphate-4-deoxy-4-fluoro-alpha-d-galactose: 179, 199, 200, 216, 217, 218, 231, 233, 292, 295, 299

1naiA Udp-galactose 4-epimerase from escherichia coli, oxidized (see paper)
27% identity, 51% coverage: 3:145/278 of query aligns to 2:159/338 of 1naiA

query
sites
1naiA

K

R

I

V

A

L

L

V

S

T

G
|
G

A

G

G

S

G
|
G

Q
x
Y

L
x
I

G

G

S

S

V

H

V

T

R

C

A

V

A

Q

L

L

I

L

A

Q

R

N

G

G

T

H

P

D

-

V

-

I

L

L

R
x
D

S
x
N

A
x
L

A
x
C

G
x
N

S
|
S

K

K

-

R

A

S

L

V

V

L

P

P

L

V

V

I

D

E

-

R

-

L

-

G

-

K

G

H

E

P

D

T

V

F

M

V

H

E

G

G

D
|
D

L
x
I

R

R

D

N

P

E

A

A

V

L

V

M

D

T

R

E

L

I

L

L

E

H

-

D

-

H

G

A

V

I

D

D

V

T

L

V

I

I

H

H

F
|
F

A
|
A

G
|
G

-

L

-
x
K

-

A

-

V

-

G

T

E

S

S

V

V

E

Q

R

K

P

P

L

L

P

-

E

E

I

Y

E

D

N

N

L

V

R

N

G

G

L

T

V

L

E

R

V

L

Y

I

E

S

G

A

A

M

R

R

R

A

Q

A

G

N

V

V

K

K

R

N

I

F

V

I

F

F

A

S

S

S

S
|
S

N
x
A

H
x
T

A

V

I

Y

G

G

M

D

Y

N

P

P

V

K

T

I

E

P

H

Y

L

V

S

E

L

S

D

F

C

P

E

T

L

G

R

T

P

P

D

Q

G

S

F

P

Y
|
Y

G

G

L

K

S

S

K
|
K

V

L

W

M

G

V

E

E

A

Q

L

I

active site: S124 (= S110), A125 (≠ N111), T126 (≠ H112), Y149 (= Y135), K153 (= K139)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), Y11 (≠ Q12), I12 (≠ L13), D31 (≠ R29), N32 (≠ S30), L33 (≠ A31), C34 (≠ A32), N35 (≠ G33), S36 (= S34), D58 (= D50), I59 (≠ L51), F80 (= F70), A81 (= A71), G82 (= G72), K84 (vs. gap), Y149 (= Y135), K153 (= K139)
binding 1,3-propandiol: N35 (≠ G33), K84 (vs. gap)

Sites not aligning to the query:

active site: 189
binding nicotinamide-adenine-dinucleotide: 177, 180
binding 1,3-propandiol: 191, 193
binding uridine-5'-diphosphate: 179, 199, 200, 215, 216, 231, 233, 292

1lrjA Crystal structure of e. Coli udp-galactose 4-epimerase complexed with udp-n-acetylglucosamine (see paper)
27% identity, 51% coverage: 3:145/278 of query aligns to 2:159/338 of 1lrjA

query
sites
1lrjA

K

R

I

V

A

L

L

V

S

T

G
|
G

A

G

G

S

G
|
G

Q
x
Y

L
x
I

G

G

S

S

V

H

V

T

R

C

A

V

A

Q

L

L

I

L

A

Q

R

N

G

G

T

H

P

D

-

V

-

I

L

L

R
x
D

S
x
N

A
x
L

A
x
C

G
x
N

S
|
S

K

K

-

R

A

S

L

V

V

L

P

P

L

V

V

I

D

E

-

R

-

L

-

G

-

K

G

H

E

P

D

T

V

F

M

V

H

E

G

G

D
|
D

L
x
I

R

R

D

N

P

E

A

A

V

L

V

M

D

T

R

E

L

I

L

L

E

H

-

D

-

H

G

A

V

I

D

D

V

T

L

V

I

I

H

H

F
|
F

A
|
A

G
|
G

-

L

-
x
K

-

A

-
x
V

-

G

T

E

S

S

V

V

E

Q

R

K

P

P

L

L

P

-

E

E

I

Y

E

D

N

N

L

V

R

N

G

G

L

T

V

L

E

R

V

L

Y

I

E

S

G

A

A

M

R

R

R

A

Q

A

G

N

V

V

K

K

R

N

I

F

V

I

F

F

A

S

S

S

S
|
S

N
x
A

H
x
T

A

V

I

Y

G

G

M

D

Y

N

P

P

V

K

T

I

E

P

H

Y

L

V

S

E

L

S

D

F

C

P

E

T

L

G

R

T

P

P

D

Q

G

S

F

P

Y
|
Y

G

G

L

K

S

S

K
|
K

V

L

W

M

G

V

E

E

A

Q

L

I

active site: S124 (= S110), A125 (≠ N111), T126 (≠ H112), Y149 (= Y135), K153 (= K139)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), Y11 (≠ Q12), I12 (≠ L13), D31 (≠ R29), N32 (≠ S30), L33 (≠ A31), C34 (≠ A32), N35 (≠ G33), S36 (= S34), D58 (= D50), I59 (≠ L51), F80 (= F70), A81 (= A71), G82 (= G72), K84 (vs. gap), Y149 (= Y135), K153 (= K139)
binding uridine-diphosphate-n-acetylglucosamine: V86 (vs. gap), S124 (= S110)

Sites not aligning to the query:

active site: 189
binding nicotinamide-adenine-dinucleotide: 177, 180
binding uridine-diphosphate-n-acetylglucosamine: 178, 179, 200, 215, 216, 218, 231, 233, 269, 292, 295

1kvrA Udp-galactose 4-epimerase complexed with udp-phenol (see paper)
26% identity, 51% coverage: 3:145/278 of query aligns to 2:159/338 of 1kvrA

query
sites
1kvrA

K

R

I

V

A

L

L

V

S

T

G
|
G

A

G

G

S

G
|
G

Q
x
Y

L
x
I

G

G

S

S

V

H

V

T

R

C

A

V

A

Q

L

L

I

L

A

Q

R

N

G

G

T

H

P

D

-

V

-

I

L

L

R
x
D

S
x
N

A

L

A
x
C

G
x
N

S
|
S

K

K

-

R

A

S

L

V

V

L

P

P

L

V

V

I

D

E

-

R

-

L

-

G

-

K

G

H

E

P

D

T

V

F

M

V

H

E

G

G

D
|
D

L
x
I

R

R

D

N

P

E

A

A

V

L

V

M

D

T

R

E

L

I

L

L

E

H

-

D

-

H

G

A

V

I

D

D

V

T

L

V

I

I

H

H

F
|
F

A
|
A

G
|
G

-

L

-
x
K

-

A

-

V

-

G

T

E

S

S

V

V

E

Q

R

K

P

P

L

L

P

-

E

E

I

Y

E

D

N

N

L

V

R

N

G

G

L

T

V

L

E

R

V

L

Y

I

E

S

G

A

A

M

R

R

R

A

Q

A

G

N

V

V

K

K

R

N

I

F

V

I

F

F

A
x
S

S
|
S

S
x
A

N
x
A

H
x
T

A

V

I

Y

G

G

M

D

Y

Q

P

P

V

K

T

I

E

P

H

Y

L

V

S

E

L

S

D

F

C

P

E

T

L

G

R

T

P

P

D

Q

G

S

F

P

Y
|
Y

G

G

L

K

S

S

K
|
K

V

L

W

M

G

V

E

E

A

Q

L

I

active site: A124 (≠ S110), A125 (≠ N111), T126 (≠ H112), Y149 (= Y135), K153 (= K139)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), Y11 (≠ Q12), I12 (≠ L13), D31 (≠ R29), N32 (≠ S30), C34 (≠ A32), N35 (≠ G33), S36 (= S34), D58 (= D50), I59 (≠ L51), F80 (= F70), A81 (= A71), G82 (= G72), K84 (vs. gap), S122 (≠ A108), S123 (= S109), Y149 (= Y135), K153 (= K139)

Sites not aligning to the query:

active site: 189
binding nicotinamide-adenine-dinucleotide: 177, 180
binding uridine-5'-diphosphate: 179, 198, 199, 200, 216, 217, 218, 231, 233, 269, 292, 295

1a9yA Udp-galactose 4-epimerase mutant s124a/y149f complexed with udp- glucose (see paper)
26% identity, 51% coverage: 3:145/278 of query aligns to 2:159/338 of 1a9yA

query
sites
1a9yA

K

R

I

V

A

L

L

V

S

T

G
|
G

A

G

G

S

G
|
G

Q
x
Y

L
x
I

G

G

S

S

V

H

V

T

R

C

A

V

A

Q

L

L

I

L

A

Q

R

N

G

G

T

H

P

D

-

V

-

I

L

L

R
x
D

S
x
N

A

L

A
x
C

G
x
N

S
|
S

K

K

-

R

A

S

L

V

V

L

P

P

L

V

V

I

D

E

-

R

-

L

-

G

-

K

G

H

E

P

D

T

V

F

M

V

H

E

G

G

D
|
D

L
x
I

R

R

D

N

P

E

A

A

V

L

V

M

D

T

R

E

L

I

L

L

E

H

-

D

-

H

G

A

V

I

D

D

V

T

L

V

I

I

H

H

F
|
F

A
|
A

G
|
G

-

L

-
x
K

-

A

-

V

-

G

T

E

S

S

V

V

E

Q

R

K

P

P

L

L

P

-

E

E

I

Y

E

D

N

N

L

V

R

N

G

G

L

T

V

L

E

R

V

L

Y

I

E

S

G

A

A

M

R

R

R

A

Q

A

G

N

V

V

K

K

R

N

I

F

V

I

F

F

A
x
S

S
|
S

S
x
A

N
x
A

H
x
T

A

V

I

Y

G

G

M

D

Y

Q

P

P

V

K

T

I

E

P

H

Y

L

V

S

E

L

S

D

F

C

P

E

T

L

G

R

T

P

P

D

Q

G

S

F

P

Y
x
F

G

G

L

K

S

S

K
|
K

V

L

W

M

G

V

E

E

A

Q

L

I

active site: A124 (≠ S110), A125 (≠ N111), T126 (≠ H112), F149 (≠ Y135), K153 (= K139)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), Y11 (≠ Q12), I12 (≠ L13), D31 (≠ R29), N32 (≠ S30), C34 (≠ A32), N35 (≠ G33), S36 (= S34), D58 (= D50), I59 (≠ L51), F80 (= F70), A81 (= A71), G82 (= G72), K84 (vs. gap), S122 (≠ A108), S123 (= S109), F149 (≠ Y135), K153 (= K139)
binding uridine-5'-diphosphate-glucose: A125 (≠ N111), T126 (≠ H112)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 177, 180
binding uridine-5'-diphosphate-glucose: 179, 199, 200, 216, 217, 218, 231, 233, 292, 295, 299

Q7BJX9 UDP-N-acetylglucosamine 4-epimerase; UDP-GalNAc 4-epimerase; EC 5.1.3.7 from Plesiomonas shigelloides (Aeromonas shigelloides) (see 2 papers)
24% identity, 45% coverage: 47:172/278 of query aligns to 78:206/345 of Q7BJX9

query
sites
Q7BJX9

M

I

H

E

G

G

D
|
D

L
x
I

R

R

D

D

P

L

A

T

V

T

V

C

D

E

R

Q

L

V

L

M

E

K

G

G

V

V

D

D

V

H

L

V

I

L

H

H

F
x
Q

A
|
A

G
x
A

T

L

-

G

S

S

V

V

E

P

R

R

P

S

L

I

P

V

E

D

I

P

I

I

E

T

-

T

-

N

-

A

N
x
T

N

N

L

I

R

T

G

G

L

F

V

L

E

N

V

I

Y

L

E

H

G

A

A

A

R

K

R

N

Q

A

G

Q

V

V

K

Q

R

S

I

F

V

T

F

Y

A

A

S

A

S
|
S

N
x
S

H
x
S

A

T

I

Y

G

G

M

D

Y

H

P

P

V

A

T

L

E

P

H

K

L

V

S

E

L

E

D

N

C

I

E

G

L

-

R

N

P

P

D

L

G

S

F

P

Y
|
Y

G

A

L

V

S

T

K
|
K

V

Y

W

V

G

N

E

E

A

I

L

Y

A

A

R

Q

M

V

Y

Y

W

A

D

R

K

T

H

Y

G

G

I

F

E

K

S

T

V

I

C

G

V

L

R

R

I
x
Y

G
x
F

S
x
N

C
x
V

L

F

E

G

R

R

P

R

T

Q

E

D

P

P

DI 81:82 (≠ DL 50:51) binding
QAA 101:103 (≠ FAG 70:72) binding
T120 (≠ N85) binding
SS 145:146 (≠ SN 110:111) binding
S147 (≠ H112) mutation to T: No effect on epimerase activity.
Y169 (= Y135) binding
K173 (= K139) binding
YFN 196:198 (≠ IGS 162:164) binding
V199 (≠ C165) binding

Sites not aligning to the query:

26:31 binding
50:55 binding
213:216 binding
228:230 binding
236 S→G: No effect on epimerase activity.
237 binding
271 R→G: No effect on epimerase activity.
302:305 binding
307 R→A: No effect on epimerase activity.
308 H→A: No effect on epimerase activity.
309 S→Y: Abolishes epimerase activity.

3ruhA Alternative analogs as viable substrates of udp-hexose 4-epimerases
24% identity, 45% coverage: 47:172/278 of query aligns to 75:203/336 of 3ruhA

query
sites
3ruhA

M

I

H

E

G

G

D
|
D

L
x
I

R

R

D

D

P

L

A

T

V

T

V

C

D

E

R

Q

L

V

L

M

E

K

G

G

V

V

D

D

V

H

L

V

I

L

H

H

F
x
Q

A
|
A

G
x
A

T

L

-
x
G

S
|
S

V

V

E

P

R

R

P

S

L

I

P

V

E

D

I

P

I

I

E

T

-

T

-

N

-

A

N
x
T

N

N

L

I

R

T

G

G

L

F

V

L

E

N

V

I

Y

L

E

H

G

A

A

A

R

K

R

N

Q

A

G

Q

V

V

K

Q

R

S

I

F

V

T

F

Y

A
|
A

S
x
A

S
|
S

N
x
S

H
x
S

A

T

I

Y

G

G

M

D

Y

H

P

P

V

A

T

L

E

P

H

K

L

V

S

E

L

E

D

N

C

I

E

G

L

-

R

N

P

P

D

L

G

S

F

P

Y
|
Y

G

A

L

V

S

T

K
|
K

V

Y

W

V

G

N

E

E

A

I

L

Y

A

A

R

Q

M

V

Y

Y

W

A

D

R

K

T

H

Y

G

G

I

F

E

K

S

T

V

I

C

G

V

L

R

R

I
x
Y

G

F

S
x
N

C
x
V

L

F

E

G

R

R

P

R

T

Q

E

D

P

P

active site: S142 (= S110), S143 (≠ N111), S144 (≠ H112), Y166 (= Y135), K170 (= K139)
binding nicotinamide-adenine-dinucleotide: D78 (= D50), I79 (≠ L51), Q98 (≠ F70), A99 (= A71), A100 (≠ G72), T117 (≠ N85), A140 (= A108), A141 (≠ S109), S142 (= S110), Y166 (= Y135), K170 (= K139), Y193 (≠ I162), V196 (≠ C165)
binding [[(2R,3S,4R,5R)-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl][(2R,3R,4R,5R,6R)-6-(hydroxymethyl)-4,5-bis(oxidanyl)-3-(2-oxidanylidenepropyl)oxan-2-yl] hydrogen phosphate: G102 (vs. gap), S103 (= S74), S142 (= S110), S143 (≠ N111), S144 (≠ H112), Y166 (= Y135), Y193 (≠ I162), N195 (≠ S164)

Sites not aligning to the query:

3rufA Alternative analogs as viable substrates of udp-hexose 4-epimerases
24% identity, 45% coverage: 47:172/278 of query aligns to 75:203/336 of 3rufA

query
sites
3rufA

M

I

H

E

G

G

D
|
D

L
x
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active site: S142 (= S110), S143 (≠ N111), S144 (≠ H112), Y166 (= Y135), K170 (= K139)
binding nicotinamide-adenine-dinucleotide: D78 (= D50), I79 (≠ L51), Q98 (≠ F70), A99 (= A71), A100 (≠ G72), T117 (≠ N85), A140 (= A108), Y166 (= Y135), K170 (= K139), Y193 (≠ I162), V196 (≠ C165)
binding uridine-5'-diphosphate: N195 (≠ S164)

Sites not aligning to the query:

active site: 204
binding nicotinamide-adenine-dinucleotide: 23, 26, 27, 28, 47, 48, 50, 51, 52
binding uridine-5'-diphosphate: 209, 210, 213, 214, 225, 226, 227, 234, 271, 294, 297

3lu1A Crystal structure analysis of wbgu: a udp-galnac 4-epimerase (see paper)
24% identity, 45% coverage: 47:172/278 of query aligns to 75:203/336 of 3lu1A

query
sites
3lu1A

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active site: S142 (= S110), S143 (≠ N111), S144 (≠ H112), Y166 (= Y135), K170 (= K139)
binding glycine: Q135 (≠ K103), K187 (≠ E156)
binding nicotinamide-adenine-dinucleotide: D78 (= D50), I79 (≠ L51), Q98 (≠ F70), A99 (= A71), A100 (≠ G72), A140 (= A108), A141 (≠ S109), S142 (= S110), Y166 (= Y135), K170 (= K139), Y193 (≠ I162), N195 (≠ S164)
binding uridine-diphosphate-n-acetylgalactosamine: S103 (= S74), S142 (= S110), S143 (≠ N111), S144 (≠ H112), Y166 (= Y135), N195 (≠ S164)

Sites not aligning to the query:

active site: 204
binding nicotinamide-adenine-dinucleotide: 23, 26, 27, 28, 47, 48, 50, 51, 52
binding uridine-diphosphate-n-acetylgalactosamine: 210, 214, 225, 226, 227, 234, 271, 294, 297

Query Sequence

>BPHYT_RS24115 FitnessBrowser__BFirm:BPHYT_RS24115
MKKIALSGAGGQLGSVVRAALIARGTPLRSAAGSKALVPLVDGEDVMHGDLRDPAVVDRL
LEGVDVLIHFAGTSVERPLPEIIENNLRGLVEVYEGARRQGVKRIVFASSNHAIGMYPVT
EHLSLDCELRPDGFYGLSKVWGEALARMYWDKHGIESVCVRIGSCLERPTEPRHLSTWFG
HRDLLHFLDRCVEAENVGFLTIWGVSANTRSWWDNSGAERLGYQPTQDAEVYAEEILARP
NPLDALGQRFQGGSFVGIDYSRDDAAADGSAASATRPV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory