SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS24165 FitnessBrowser__BFirm:BPHYT_RS24165 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q7CRQ0 Uronate dehydrogenase; D-galacturonate dehydrogenase; D-glucuronate dehydrogenase; Hexuronate dehydrogenase; EC 1.1.1.203 from Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)) (see paper)
49% identity, 92% coverage: 15:271/280 of query aligns to 1:257/265 of Q7CRQ0

query
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Q7CRQ0

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Y134 (= Y148) mutation to A: 0.1% of wild-type activity.

3rfvA Crystal structure of uronate dehydrogenase from agrobacterium tumefaciens complexed with nadh and product (see paper)
49% identity, 92% coverage: 15:271/280 of query aligns to 2:258/265 of 3rfvA

query
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3rfvA

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binding D-galactaro-1,5-lactone: S74 (= S87), S110 (= S123), N111 (= N124), H112 (= H125), Y135 (= Y148), S164 (= S177), R173 (= R186), F257 (= F270)
binding 1,4-dihydronicotinamide adenine dinucleotide: G9 (= G22), G12 (= G25), Q13 (≠ N26), L14 (= L27), D33 (= D46), L34 (≠ I47), D50 (= D63), L51 (≠ I64), L70 (= L83), G71 (= G84), G72 (= G85), S74 (= S87), A108 (= A121), S110 (= S123), Y135 (= Y148), K139 (= K152), I162 (= I175), S164 (= S177), C165 (≠ S178)

3rfxA Crystal structure of uronate dehydrogenase from agrobacterium tumefaciens, y136a mutant complexed with NAD (see paper)
49% identity, 92% coverage: 15:271/280 of query aligns to 2:258/265 of 3rfxA

query
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3rfxA

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binding nicotinamide-adenine-dinucleotide: G9 (= G22), G12 (= G25), Q13 (≠ N26), L14 (= L27), D33 (= D46), L34 (≠ I47), D50 (= D63), L51 (≠ I64), G72 (= G85), S74 (= S87)

4id9A Crystal structure of a short-chain dehydrogenase/reductase superfamily protein from agrobacterium tumefaciens (target efi-506441) with bound NAD, monoclinic form 1
36% identity, 56% coverage: 18:175/280 of query aligns to 2:159/321 of 4id9A

query
sites
4id9A

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active site: S106 (= S123), Y132 (= Y148), K136 (= K152)
binding nicotinamide-adenine-dinucleotide: G9 (= G25), R10 (≠ N26), V11 (≠ L27), D30 (= D46), L31 (≠ I47), R32 (≠ A48), S44 (≠ D63), L45 (≠ I64), L64 (= L83), A66 (≠ G85), V81 (≠ A98), S106 (= S123), Y132 (= Y148), K136 (= K152), F159 (≠ I175)

Sites not aligning to the query:

4id9B Crystal structure of a short-chain dehydrogenase/reductase superfamily protein from agrobacterium tumefaciens (target efi-506441) with bound NAD, monoclinic form 1
36% identity, 56% coverage: 18:175/280 of query aligns to 3:160/328 of 4id9B

query
sites
4id9B

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active site: S107 (= S123), Y133 (= Y148), K137 (= K152)
binding nicotinamide-adenine-dinucleotide: G10 (= G25), R11 (≠ N26), V12 (≠ L27), D31 (= D46), L32 (≠ I47), S45 (≠ D63), L46 (≠ I64), L65 (= L83), A67 (≠ G85), V82 (≠ A98), Y133 (= Y148), K137 (= K152), F160 (≠ I175)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 161, 162, 163

7cgvA Full consensus l-threonine 3-dehydrogenase, fctdh-iiym (NAD+ bound form) (see paper)
29% identity, 57% coverage: 16:174/280 of query aligns to 1:165/310 of 7cgvA

query
sites
7cgvA

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P

H

F

E

E

E

-

A

-

S

-

I

Y

V
x
L

D
|
D

I
x
V

A

L

D

D

E

K

A

E

A

A

V

L

H

A

A

E

L

L

L

V

E

K

G

K

-

Y

-

K

V

I

D

T

A

Q

V

I

V

Y

H

H

L

L

G
x
A

I

L

-
x
L

-

S

-

A

S

T

V

G

E

E

A

K

P

N

F

P

E

Q

D

K

L

A

L

W

Q

D

A

L

N

N

I

M

R

D

G

G

L

L

Y

L

N

N

L

V

Y

L

S

E

A

I

A

A

Q

R

K

E

Q

R

G

G

V

P

K

L

R

K

I

V

V

F

Y

W

A
x
P

S

S

S
|
S

N

I

H

A

A

A

V

F

G

G

F

P

H

N

P

T

V

P

T

K

E

D

V

N

V

T

D

P

I

Q

D

D

A

T

P

I

L

M

R

R

P

P

D

T

S

T

L

I

Y
|
Y

G

G

V

I

T

S

K
|
K

C

V

F

A

G

G

E

E

S

L

L

L

S

C

R

E

Y

Y

F

H

D

T

R

K

F

Y

G

G

L

V

E

D

T

V

V

R

C

S

L

V

R

R

binding nicotinamide-adenine-dinucleotide: G7 (= G22), G10 (= G25), Q11 (≠ N26), I12 (≠ L27), D33 (= D46), I34 (= I47), R35 (≠ A48), L48 (≠ V62), D49 (= D63), V50 (≠ I64), A72 (≠ G84), A73 (≠ G85), L75 (vs. gap), P112 (≠ A121), S114 (= S123), Y139 (= Y148), K143 (= K152)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 166, 169

3a4vA Crystal structure of pyruvate bound l-threonine dehydrogenase from hyperthermophilic archaeon thermoplasma volcanium (see paper)
28% identity, 56% coverage: 18:174/280 of query aligns to 2:163/311 of 3a4vA

query
sites
3a4vA

L

I

L

L

L

V

T

T

G

G

A

S

A
x
S

G
|
G

N
x
Q

L
x
I

G

G

K

T

Q

E

L

L

R

V

G

P

A

Y

L

L

A

A

A

E

W

K

-

Y

-

G

-

K

-

N

-

V

-

I

-

A

A

S

D
|
D

I
|
I

V

V

R

Q

I

-

T

R

D

D

I

T

A

G

P

G

L

I

G

K

E

F

V

I

A

T

A

-

H

-

E

-

A

-

S

-

I

-

V
x
L

D
|
D

I
x
V

A

S

D

N

E

R

A

D

A

E

V

I

H

D

A

R

L

A

L

V

E

E

-

K

-

Y

G

S

V

I

D

D

A

A

V

I

V

F

H

H

L
|
L

G
x
A

G
|
G

I

I

S
x
L

V
x
S

E

A

A

K

P

G

F

E

E

K

D

D

-

P

-

A

-

L

L

A

L

Y

Q

K

A

V

N

N

I

M

R

N

G

G

L

T

Y

Y

N

N

L

I

Y

L

S

E

A

A

Q

K

K

Q

Q

H

G

R

V

V

K

E

R

K

I

V

V

V

Y

I

A
x
P

S
|
S

S
x
T

N

I

H

G

A

V

V

F

G

G

F

P

H

E

P

T

V

P

T

K

E

N

V

K

V

V

D

P

I

S

D

I

A

T

P

I

L

T

R

R

P

P

D

R

S

T

L

M

Y
|
Y

G

G

V

V

T

T

K
|
K

C

I

F

A

G

A

E

E

S

L

L

L

S

G

R

Q

Y

Y

F

Y

D

E

R

K

F

F

G

G

L

L

E

D

T

V

V

R

C

S

L

L

R

R

active site: T112 (≠ S123), Y137 (= Y148), K141 (= K152)
binding nicotinamide-adenine-dinucleotide: S8 (≠ A24), G9 (= G25), Q10 (≠ N26), I11 (≠ L27), D32 (= D40), I33 (= I41), L46 (≠ V62), D47 (= D63), V48 (≠ I64), L69 (= L83), A70 (≠ G84), G71 (= G85), L73 (≠ S87), P110 (≠ A121), S111 (= S122), T112 (≠ S123), Y137 (= Y148), K141 (= K152)
binding pyruvic acid: S74 (≠ V88), Y137 (= Y148)

Sites not aligning to the query:

3a1nA Crystal structure of l-threonine dehydrogenase from hyperthermophilic archaeon thermoplasma volcanium (see paper)
28% identity, 56% coverage: 18:174/280 of query aligns to 2:163/315 of 3a1nA

query
sites
3a1nA

L

I

L

L

L

V

T

T

G

G

A

S

A
x
S

G
|
G

N
x
Q

L
x
I

G

G

K

T

Q

E

L

L

R

V

G

P

A

Y

L

L

A

A

A

E

W

K

-

Y

-

G

-

K

-

N

-

V

-

I

-

A

A

S

D
|
D

I
|
I

V

V

R

Q

I

-

T

R

D

D

I

T

A

G

P

G

L

I

G

K

E

F

V

I

A

T

A

-

H

-

E

-

A

-

S

-

I

-

V
x
L

D
|
D

I

V

A

S

D

N

E

R

A

D

A

E

V

I

H

D

A

R

L

A

L

V

E

E

-

K

-

Y

G

S

V

I

D

D

A

A

V

I

V

F

H

H

L
|
L

G
x
A

G
|
G

I

I

S
x
L

V

S

E

A

A

K

P

G

F

E

E

K

D

D

-

P

-

A

-

L

L

A

L

Y

Q

K

A

V

N

N

I

M

R

N

G

G

L

T

Y

Y

N

N

L

I

Y

L

S

E

A

A

Q

K

K

Q

Q

H

G

R

V

V

K

E

R

K

I

V

V

V

Y

I

A

P

S
|
S

S
x
T

N

I

H

G

A

V

V

F

G

G

F

P

H

E

P

T

V

P

T

K

E

N

V

K

V

V

D

P

I

S

D

I

A

T

P

I

L

T

R

R

P

P

D

R

S

T

L

M

Y
|
Y

G

G

V

V

T

T

K
|
K

C

I

F

A

G

A

E

E

S

L

L

L

S

G

R

Q

Y

Y

F

Y

D

E

R

K

F

F

G

G

L

L

E

D

T

V

V

R

C

S

L

L

R

R

active site: T112 (≠ S123), Y137 (= Y148), K141 (= K152)
binding nicotinamide-adenine-dinucleotide: S8 (≠ A24), G9 (= G25), Q10 (≠ N26), I11 (≠ L27), D32 (= D40), I33 (= I41), L46 (≠ V62), D47 (= D63), L69 (= L83), A70 (≠ G84), G71 (= G85), L73 (≠ S87), S111 (= S122), Y137 (= Y148), K141 (= K152)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 164

6wjaA Udp-glcnac c4-epimerase mutant s121a/y146f from pseudomonas protegens in complex with udp-galnac (see paper)
32% identity, 60% coverage: 17:185/280 of query aligns to 2:180/307 of 6wjaA

query
sites
6wjaA

R

R

L

I

L

L

L

V

T

T

G
|
G

A

G

A

A

G
|
G

N
x
F

L
x
I

G

G

K

S

Q

H

L

L

R

V

G

D

A

A

L

L

A

L

A

A

W

K

A

G

D

Y

I

A

V

V

R

R

I

V

T

L

D
|
D

I
x
D

A

L

P
x
S

L
x
T

G
|
G

E

K

V

V

A

G

-

N

-

L

-

P

-

M

-

G

-

D

A

A

H

G

E

L

E

E

A

L

S

L

I

V

V

G

D

D

I
x
A

A

A

D

D

E

A

A

A

A

L

V

L

H

A

A

D

L

A

L

V

E

Q

G

G

V

C

D

D

A

A

V

V

H

H

L
|
L

G
x
A

I

V

-

A

S

S

V
|
V

E

Q

A

A

P

S

F

V

E

E

D

D

L

P

L

V

-

A

-

T

-

H

Q

Q

A
x
S

N

N

I

F

R

I

G

A

L

T

Y

L

N

R

L

L

Y

C

S

E

A

A

A

M

Q

T

K

A

Q

A

G

G

V

I

K

R

R

R

I

V

V

V

Y

F

A

A

S

S

S
x
A

N
x
A

H
x
A

A

V

V

Y

G

G

F

N

H

N

P

G

V

E

T

G

E

T

V

P

V

I

D

A

I

E

D

D

A

T

P

P

L

K

R

S

P

P

D

L

S

T

L

P

Y
x
F

G

A

V

A

T

D

K
|
K

C

L

F

A

G

S

E

E

S

Y

L

Y

S

L

R

D

Y

F

Y

Y

F

R

D

R

R

Q

F

H

G

G

L

L

E

E

T

P

V

V

C

I

L

L

R

R

I
x
F

G
x
F

S
x
N

S
x
I

F

F

E

G

Q

P

P

R

K

Q

N

D

P

P

active site: A118 (≠ S123), A119 (≠ N124), A120 (≠ H125), F143 (≠ Y148), K147 (= K152)
binding nicotinamide-adenine-dinucleotide: G7 (= G22), G10 (= G25), F11 (≠ N26), I12 (≠ L27), D31 (= D46), D32 (≠ I47), S34 (≠ P49), T35 (≠ L50), G36 (= G51), A55 (≠ I64), L74 (= L83), A75 (≠ G84), A76 (≠ G85), S93 (≠ A98), F143 (≠ Y148), K147 (= K152), F170 (≠ I175), F171 (≠ G176), I173 (≠ S178)
binding uridine-diphosphate-n-acetylgalactosamine: V80 (= V88), A120 (≠ H125), N172 (≠ S177)

Sites not aligning to the query:

binding uridine-diphosphate-n-acetylgalactosamine: 186, 187, 191, 202, 204, 211, 247, 270, 273

6wj9B Udp-glcnac c4-epimerase mutant s121a/y146f from pseudomonas protegens in complex with udp-glcnac (see paper)
32% identity, 60% coverage: 17:185/280 of query aligns to 3:181/308 of 6wj9B

query
sites
6wj9B

R

R

L

I

L

L

L

V

T

T

G
|
G

A

G

A

A

G
|
G

N
x
F

L
x
I

G

G

K

S

Q

H

L

L

R

V

G

D

A

A

L

L

A

L

A

A

W

K

A

G

D

Y

I

A

V

V

R

R

I

V

T

L

D
|
D

I
x
D

A

L

P
x
S

L
x
T

G
|
G

E

K

V

V

A

G

-

N

-

L

-

P

-

M

-

G

-

D

A

A

H

G

E

L

E

E

A

L

S

L

I

V

V

G

D
|
D

I
x
A

A

A

D

D

E

A

A

A

A

L

V

L

H

A

A

D

L

A

L

V

E

Q

G

G

V

C

D

D

A

A

V

V

H

H

L
|
L

G
x
A

I

V

-

A

S

S

V
|
V

E

Q

A

A

P

S

F

V

E

E

D

D

L

P

L

V

-

A

-

T

-

H

Q

Q

A
x
S

N

N

I

F

R

I

G

A

L

T

Y

L

N

R

L

L

Y

C

S

E

A

A

A

M

Q

T

K

A

Q

A

G

G

V

I

K

R

R

R

I

V

V

V

Y

F

A
|
A

S

S

S
x
A

N
x
A

H
x
A

A

V

V

Y

G

G

F

N

H

N

P

G

V

E

T

G

E

T

V

P

V

I

D

A

I

E

D

D

A

T

P

P

L

K

R

S

P

P

D

L

S

T

L

P

Y
x
F

G

A

V

A

T

D

K
|
K

C

L

F

A

G

S

E

E

S

Y

L

Y

S

L

R

D

Y

F

Y

Y

F

R

D

R

R

Q

F

H

G

G

L

L

E

E

T

P

V

V

C

I

L

L

R

R

I
x
F

G
x
F

S
x
N

S
x
I

F

F

E

G

Q

P

P

R

K

Q

N

D

P

P

active site: A119 (≠ S123), A120 (≠ N124), A121 (≠ H125), F144 (≠ Y148), K148 (= K152)
binding nicotinamide-adenine-dinucleotide: G8 (= G22), G11 (= G25), F12 (≠ N26), I13 (≠ L27), D32 (= D46), D33 (≠ I47), S35 (≠ P49), T36 (≠ L50), G37 (= G51), D55 (= D63), A56 (≠ I64), L75 (= L83), A76 (≠ G84), A77 (≠ G85), S94 (≠ A98), A117 (= A121), A119 (≠ S123), F144 (≠ Y148), K148 (= K152), F171 (≠ I175), F172 (≠ G176), I174 (≠ S178)
binding uridine-diphosphate-n-acetylglucosamine: V81 (= V88), N173 (≠ S177)

Sites not aligning to the query:

binding uridine-diphosphate-n-acetylglucosamine: 187, 188, 192, 203, 204, 205, 212, 248, 271, 274

3ajrA Crystal structure of l-3-hydroxynorvaline bound l-threonine dehydrogenase (y137f) from hyperthermophilic archaeon thermoplasma volcanium (see paper)
28% identity, 56% coverage: 18:174/280 of query aligns to 2:163/314 of 3ajrA

query
sites
3ajrA

L

I

L

L

L

V

T

T

G

G

A

S

A
x
S

G
|
G

N
x
Q

L
x
I

G

G

K

T

Q

E

L

L

R

V

G

P

A

Y

L

L

A

A

A

E

W

K

-

Y

-

G

-

K

-

N

-

V

-

I

-

A

A

S

D
|
D

I
|
I

V

V

R

Q

I

-

T

R

D

D

I

T

A

G

P

G

L

I

G

K

E

F

V

I

A

T

A

-

H

-

E

-

A

-

S

-

I

-

V
x
L

D
|
D

I
x
V

A

S

D

N

E

R

A

D

A

E

V

I

H

D

A

R

L

A

L

V

E

E

-

K

-

Y

G

S

V

I

D

D

A

A

V

I

V

F

H

H

L
|
L

G
x
A

G
|
G

I

I

S
x
L

V
x
S

E

A

A

K

P

G

F

E

E

K

D

D

-

P

-

A

-

L

L

A

L

Y

Q

K

A

V

N

N

I

M

R

N

G

G

L

T

Y

Y

N

N

L

I

Y

L

S

E

A

A

Q

K

K

Q

Q

H

G

R

V

V

K

E

R

K

I

V

V

V

Y

I

A
x
P

S
|
S

S
x
T

N
x
I

H

G

A

V

V

F

G

G

F

P

H

E

P

T

V

P

T

K

E

N

V

K

V

V

D

P

I

S

D

I

A

T

P

I

L

T

R

R

P

P

D

R

S

T

L

M

Y
x
F

G

G

V

V

T

T

K
|
K

C

I

F

A

G

A

E

E

S

L

L

L

S

G

R

Q

Y

Y

F

Y

D

E

R

K

F

F

G

G

L

L

E

D

T

V

V

R

C

S

L

L

R

R

binding nicotinamide-adenine-dinucleotide: S8 (≠ A24), G9 (= G25), Q10 (≠ N26), I11 (≠ L27), D32 (= D40), I33 (= I41), L46 (≠ V62), D47 (= D63), V48 (≠ I64), L69 (= L83), A70 (≠ G84), G71 (= G85), L73 (≠ S87), P110 (≠ A121), S111 (= S122), T112 (≠ S123), F137 (≠ Y148), K141 (= K152)
binding (3R)-3-hydroxy-L-norvaline: S74 (≠ V88), T112 (≠ S123), I113 (≠ N124), F137 (≠ Y148)

Sites not aligning to the query:

3a9wA Crystal structure of l-threonine bound l-threonine dehydrogenase (y137f) from hyperthermophilic archaeon thermoplasma volcanium (see paper)
28% identity, 56% coverage: 18:174/280 of query aligns to 2:163/315 of 3a9wA

query
sites
3a9wA

L

I

L

L

L

V

T

T

G

G

A

S

A
x
S

G
|
G

N
x
Q

L
x
I

G

G

K

T

Q

E

L

L

R

V

G

P

A

Y

L

L

A

A

A

E

W

K

-

Y

-

G

-

K

-

N

-

V

-

I

-

A

A

S

D
|
D

I
|
I

V

V

R

Q

I

-

T

R

D

D

I

T

A

G

P

G

L

I

G

K

E

F

V

I

A

T

A

-

H

-

E

-

A

-

S

-

I

-

V
x
L

D
|
D

I
x
V

A

S

D

N

E

R

A

D

A

E

V

I

H

D

A

R

L

A

L

V

E

E

-

K

-

Y

G

S

V

I

D

D

A

A

V

I

V

F

H

H

L
|
L

G
x
A

G

G

I

I

S
x
L

V
x
S

E

A

A

K

P

G

F

E

E

K

D

D

-

P

-

A

-

L

L

A

L

Y

Q

K

A
x
V

N

N

I

M

R

N

G

G

L

T

Y

Y

N

N

L

I

Y

L

S

E

A

A

Q

K

K

Q

Q

H

G

R

V

V

K

E

R

K

I

V

V

V

Y

I

A
x
P

S
|
S

S
x
T

N

I

H

G

A

V

V

F

G

G

F

P

H

E

P

T

V

P

T

K

E

N

V

K

V

V

D

P

I

S

D

I

A

T

P

I

L

T

R

R

P

P

D

R

S

T

L

M

Y
x
F

G

G

V

V

T

T

K
|
K

C

I

F

A

G

A

E

E

S

L

L

L

S

G

R

Q

Y

Y

F

Y

D

E

R

K

F

F

G

G

L

L

E

D

T

V

V

R

C

S

L

L

R

R

binding nicotinamide-adenine-dinucleotide: S8 (≠ A24), G9 (= G25), Q10 (≠ N26), I11 (≠ L27), D32 (= D40), I33 (= I41), L46 (≠ V62), D47 (= D63), V48 (≠ I64), L69 (= L83), A70 (≠ G84), L73 (≠ S87), V87 (≠ A98), P110 (≠ A121), S111 (= S122), K141 (= K152)
binding threonine: S74 (≠ V88), T112 (≠ S123), F137 (≠ Y148)

Sites not aligning to the query:

3eheA Crystal structure of udp-glucose 4 epimerase (gale-1) from archaeoglobus fulgidus
30% identity, 57% coverage: 18:177/280 of query aligns to 2:164/290 of 3eheA

query
sites
3eheA

L

I

L

V

T

T

G
|
G

A

G

A

A

G
|
G

N
x
F

L
x
I

G

G

K

S

Q

H

L

V

R

V

G

D

A

K

L

L

A

S

A

E

W

S

A

N

D

E

I

I

V

V

R

V

I

I

T
x
D

D
x
N

I
x
L

A
x
S

P
x
S

L
x
G

G

N

E

E

V

E

A

F

A

V

H

N

E

E

E

A

A

A

S

R

I

L

V

V

D

K

I

-

A

A

D
|
D

E
x
L

A

A

-

D

V

I

H

K

A

D

L

Y

L

L

E

K

G

G

V

A

D

E

A

E

V

V

V

W

H

H

L
x
I

G
x
A

I

N

-

P

-

D

-

V

-

R

S

G

V

A

E

E

A

N

P

P

F

-

E

D

D

E

L

I

L

Y

Q

R

A

N

N

N

I

V

R

L

G

A

L

T

Y

Y

N

R

L

L

Y

L

S

E

A

A

A

M

Q

R

K

K

Q

A

G

G

V

V

K

S

R

R

I

I

V

V

Y

F

A
x
T

S

S

S
x
T

N
x
S

H
x
T

A

V

V

Y

G

G

F

E

H

A

P

K

V

V

T

I

E

P

V

T

V

P

D

E

I

-

D

D

A

Y

P

P

L

T

R

H

P

P

D

I

S

S

L

L

Y
|
Y

G

G

V

A

T

S

K
|
K

C

L

F

A

G

C

E

E

S

A

L

L

S

I

R

E

Y

S

Y

Y

F

C

D

H

R

T

F

F

G

D

L

M

E

Q

T

A

V

W

C

I

L

Y

R

R

I
x
F

G
x
A

S
x
N

active site: T111 (≠ S123), S112 (≠ N124), T113 (≠ H125), Y135 (= Y148), K139 (= K152)
binding nicotinamide-adenine-dinucleotide: G6 (= G22), G9 (= G25), F10 (≠ N26), I11 (≠ L27), D29 (≠ T45), N30 (≠ D46), L31 (≠ I47), S32 (≠ A48), S33 (≠ P49), G34 (≠ L50), D49 (= D66), L50 (≠ E67), I68 (≠ L83), A69 (≠ G84), A70 (≠ G85), T109 (≠ A121), Y135 (= Y148), K139 (= K152), F162 (≠ I175), A163 (≠ G176), N164 (≠ S177)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 165

6jygD Crystal structure of l-threonine dehydrogenase from phytophthora infestans (see paper)
31% identity, 56% coverage: 17:174/280 of query aligns to 2:162/307 of 6jygD

query
sites
6jygD

R

R

L

I

L

L

L

V

T

T

G
|
G

A

G

A
x
T

G
|
G

N
x
Q

L
x
I

G

G

K

M

Q

E

L

L

R

V

G

P

A

Y

L

M

A

R

A

Q

W

L

-

F

-

G

A

A

D

D

I

T

V

I

R

V

I

N

T

S

D
|
D

I
|
I

A
x
K

P

A

L

P

G

G

E

R

V

D

A

G

A

N

H

F

E

V

E

Y

A

C

S
x
D

I
x
V

V

Q

D

D

I

-

A

R

D

D

E

S

A

M

A

A

V

R

H

I

A

V

L

T

L

E

E

Q

G

G

V

V

D

D

A

T

V

I

V

V

H

H

L
x
M

G
x
A

G
x
S

I

L

S
x
L

V
x
S

-

A

-

V

-

G

E

E

A

A

P

N

F

P

E

S

D

L

L

A

L

L

Q

S

A
x
V

N

N

I

T

R

R

G

G

L

I

Y

Q

N

N

L

V

Y

L

S

E

A

L

A

A

Q

K

K

Q

Q

Y

G

Q

V

L

K

R

R

-

I

-

V

V

Y

F

A

A

S
x
P

S
|
S

N
x
T

H

I

A

A

V

V

G

-

F

F

H

G

P

P

V

S

T

T

-

P

-

Q

-

D

E

E

V

T

V

P

D

D

I

T

D

T

A

I

P

-

L

M

R

R

P

P

D

T

S

T

L

M

Y
|
Y

G

G

V

L

T

T

K
|
K

C

V

F

H

G

V

E

E

S

L

L

L

S

G

R

E

Y

Y

F

Y

D

N

R

K

F

F

G

G

L

V

E

D

T

F

V

R

C

S

L

V

R

R

binding citrate anion: Q11 (≠ N26), L73 (≠ S87), S74 (≠ V88), Y136 (= Y148)
binding nicotinamide-adenine-dinucleotide: G7 (= G22), T9 (≠ A24), G10 (= G25), Q11 (≠ N26), I12 (≠ L27), D33 (= D46), I34 (= I47), K35 (≠ A48), D47 (≠ S60), V48 (≠ I61), M69 (≠ L83), A70 (≠ G84), S71 (≠ G85), L73 (≠ S87), V87 (≠ A98), P109 (≠ S122), S110 (= S123), T111 (≠ N124), Y136 (= Y148), K140 (= K152)

Sites not aligning to the query:

5u4qB 1.5 angstrom resolution crystal structure of NAD-dependent epimerase from klebsiella pneumoniae in complex with NAD.
27% identity, 61% coverage: 17:187/280 of query aligns to 3:189/304 of 5u4qB

query
sites
5u4qB

R

K

L

F

L

L

L

V

T

T

G
|
G

A

A

G
|
G

N
x
F

L
x
I

G

G

K

F

Q

H

L

I

-

A

R

Q

G

R

A

L

L

L

A

N

A

E

W

G

A

H

D

N

I

V

V

V

R

G

I

I

T
x
D

D
x
N

I

M

A
x
N

P

D

L

Y

G
x
Y

E

D

V

V

A

S

A

L

H
x
K

E

Q

-

A

-

R

-

L

-

D

-

R

-

L

-

A

-

Y

-

P

-

A

-

F

E

H

A

F

S

Q

I

Q

V

L

D
|
D

I
x
L

A

A

D

D

E

R

A

E

A

G

V

M

H

A

A

K

L

L

L

F

-

A

-

T

E

E

G

Q

V

F

D

D

A

R

V

V

V

I

H

H

L
|
L

G
x
A

-
x
A

-

Q

-

A

G

G

I

V

S

R

V

L

E

E

A

N

P

P

F

Y

E

A

D

-

L

Y

L

A

Q

D

A

A

N

N

I

L

R

M

G

G

L

Y

Y

L

N

N

L

I

Y

L

S

E

A

G

A

C

Q

R

K

H

Q

T

G

K

V

V

K

K

R

H

I

L

V

V

Y

Y

A
|
A

S

S

N

S

H

S

A

V

V

Y

G

G

F

L

H

N

P

R

V

K

T

M

E

P

V

F

V

S

D

T

I

E

D

D

A

S

P

V

L

D

R

H

P

P

D

V

S

S

L

L

Y
|
Y

G

A

V

A

T

T

K
|
K

C

K

F

A

G

N

E

E

S

L

L

M

S

A

R

H

Y

T

Y

Y

F

S

D

H

R

L

F

Y

G

G

L

I

E

P

T

T

V

T

C

G

L

L

R

R

I
x
F

G

F

S

T

S
x
V

F

Y

E

G

Q

P

P

W

K

G

N
x
R

P

P

R

D

M
|
M

binding nicotinamide-adenine-dinucleotide: G8 (= G22), G11 (= G25), F12 (≠ N26), I13 (≠ L27), D32 (≠ T45), N33 (≠ D46), N35 (≠ A48), Y38 (≠ G51), K43 (≠ H56), D61 (= D63), L62 (≠ I64), L83 (= L83), A84 (≠ G84), A85 (vs. gap), A123 (= A121), Y150 (= Y148), K154 (= K152), F177 (≠ I175), V180 (≠ S178), R186 (≠ N184), M189 (= M187)

1r6dA Crystal structure of desiv double mutant (dtdp-glucose 4,6- dehydratase) from streptomyces venezuelae with NAD and dau bound (see paper)
29% identity, 62% coverage: 17:189/280 of query aligns to 2:192/322 of 1r6dA

query
sites
1r6dA

R

R

L

L

L

V

T

T

G

G

A

G

A

A

G
|
G

N
x
F

L
x
I

G

G

K

S

Q

H

L

F

R

V

G

R

A

Q

L

L

A

L

A

A

W

G

A

A

-

Y

-

P

-

D

-

V

-

P

-

A

D

D

I

E

V

V

R

I

I

V

T

L

D
|
D

I
x
S

-
x
L

-
x
T

-

Y

-
x
A

-
x
G

-

N

-

R

A

A

P

N

L

L

G

A

E

P

V

V

A

D

A

A

H

D

E

P

E

R

A

L

S

R

I

F

V

V

-

H

-

G

D
|
D

I
|
I

A

R

D

D

E

A

A

G

A

L

V

L

H

A

A

R

L

E

L

L

E

R

G

G

V

V

D

D

A

A

V

I

V

V

H

H

L
x
F

G
x
A

I

E

S
|
S

-
x
H

-

V

-

D

-

R

V

S

E

I

A

A

P

G

F

A

E

S

D

V

L

F

L

T

Q

E

A
x
T

N

N

I

V

R

Q

G

G

L

T

Y

Q

N

T

L

L

Y

L

S

Q

A

C

A

A

Q

V

K

D

Q

A

G

G

V

V

K

G

R

R

I

V

V

V

Y

H

A
x
V

S
|
S

S
x
T

N
|
N

H
x
Q

A

V

V

Y

G

G

F

S

H

I

P

D

V

S

T

G

E

S

V

W

V

T

D

E

I

-

D

S

A

S

P

P

L

L

R

E

P

P

D

N

S

S

L

P

Y
|
Y

G

A

V

A

T

S

K
|
K

C

A

F

G

G

S

E

D

S

L

L

V

S

A

R

R

Y

A

Y

Y

F

H

D

R

R

T

F

Y

G

G

L

L

E

D

T

V

V

R

C

I

L

T

R

R

I

C

G

C

S
x
N

F

Y

E

G

Q

P

P

Y

K

Q

N

H

P

P

R

E

M
x
K

L
|
L

V

I

active site: T127 (≠ S123), N128 (= N124), Q129 (≠ H125), Y151 (= Y148), K155 (= K152)
binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: S87 (= S87), H88 (vs. gap), T127 (≠ S123), N128 (= N124), Q129 (≠ H125), Y151 (= Y148), N180 (≠ S177), K190 (≠ M187), L191 (= L188)
binding nicotinamide-adenine-dinucleotide: G10 (= G25), F11 (≠ N26), I12 (≠ L27), D37 (= D46), S38 (≠ I47), L39 (vs. gap), T40 (vs. gap), A42 (vs. gap), G43 (vs. gap), D63 (= D63), I64 (= I64), F83 (≠ L83), A84 (≠ G84), A85 (≠ G85), S87 (= S87), T102 (≠ A98), V125 (≠ A121), S126 (= S122), Y151 (= Y148), K155 (= K152), N181 (≠ S178)

Sites not aligning to the query:

binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: 206, 208, 215, 250, 274, 277, 281

6pnlA Structure of epimerase mth375 from the thermophilic pseudomurein- containing methanogen methanothermobacter thermautotrophicus
30% identity, 58% coverage: 18:179/280 of query aligns to 18:190/336 of 6pnlA

query
sites
6pnlA

L

V

L

L

L

V

T

T

G
|
G

A

G

A

A

G
|
G

N
x
C

L

V

G

G

K

S

Q

N

L

L

R

T

G

G

A

N

L

L

A

A

-

K

A

A

W

G

A

A

D

N

I

V

V

I

R

I

I

L

T
x
D

D
x
N

I
x
L

A
x
S

P
x
S

L
x
S

G

Y

E

E

-

W

-

N

V

I

A

P

A

E

H

Y

E

E

E

N

A

I

S

E

I

F

V

V

-

K

-

G

D
|
D

I
|
I

A

L

D

D

E

D

A

E

A

V

V

L

H

K

A

R

L

V

L

F

-

K

E

E

G

R

V

P

D

D

A

Y

V

V

V

F

H

H

L
|
L

G

A

G
x
A

-

H

-
x
F

-

A

-

N

-

Q

I

N

S

S

V

V

E

D

A

N

P

P

F

E

E

K

D

D

L

L

Q

-

A
x
V

N

N

I

G

R

L

G

G

L

I

Y

L

N

K

L

V

Y

L

S

E

A

Y

A

A

Q

Q

K

L

Q

V

G

G

V

V

K

E

R

R

I

F

V

V

Y

Y

A
x
S

S

S

S
|
S

N

G

H
x
C

A
x
G

V

V

-

Y

G

G

F

L

H

D

P

S

V

K

T

I

E

P

V

F

V

E

D

E

I

H

D

D

A

I

P

S

L

I

R

S

P

L

D

H

S

T

L

P

Y
|
Y

G

Q

V

V

T

T

K
|
K

C

L

F

L

G

G

E

E

S

L

L

Y

S

T

R

N

Y

Y

Y

F

F

H

D

N

R

L

F

Y

G

E

L

M

E

P

T

I

V

V

C

N

L

A

R

R

I
x
F

G

F

S
x
N

S
x
V

F

F

active site: S133 (= S123), C135 (≠ H125), G136 (≠ A126), Y159 (= Y148), K163 (= K152)
binding nicotinamide-adenine-dinucleotide: G22 (= G22), G25 (= G25), C26 (≠ N26), D46 (≠ T45), N47 (≠ D46), L48 (≠ I47), S49 (≠ A48), S50 (≠ P49), S51 (≠ L50), D68 (= D63), I69 (= I64), L89 (= L83), A91 (≠ G85), F93 (vs. gap), V108 (≠ A98), S131 (≠ A121), S133 (= S123), Y159 (= Y148), K163 (= K152), F186 (≠ I175), N188 (≠ S177), V189 (≠ S178)
binding uridine-5'-diphosphate: N188 (≠ S177)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 200
binding uridine-5'-diphosphate: 201, 202, 206, 217, 218, 219, 226, 262, 285

6pmhA Structure of epimerase mth375 from the thermophilic pseudomurein- containing methanogen methanothermobacter thermautotrophicus
30% identity, 58% coverage: 18:179/280 of query aligns to 12:184/330 of 6pmhA

query
sites
6pmhA

L

V

L

L

L

V

T

T

G
|
G

A

G

A

A

G
|
G

N

C

L

V

G

G

K

S

Q

N

L

L

R

T

G

G

A

N

L

L

A

A

-

K

A

A

W

G

A

A

D

N

I

V

V

I

R

I

I

L

T
x
D

D
x
N

I

L

A
x
S

P
x
S

L
x
S

G

Y

E

E

-

W

-

N

V

I

A

P

A

E

H

Y

E

E

E

N

A

I

S

E

I

F

V

V

-

K

-

G

D
|
D

I
|
I

A

L

D

D

E

D

A

E

A

V

V

L

H

K

A

R

L

V

L

F

-

K

E

E

G

R

V

P

D

D

A

Y

V

V

V

F

H

H

L

L

G
x
A

G

A

-

H

-
x
F

-

A

-

N

-

Q

I

N

S

S

V

V

E

D

A

N

P

P

F

E

E

K

D

D

L

L

Q

-

A

V

N

N

I

G

R

L

G

G

L

I

Y

L

N

K

L

V

Y

L

S

E

A

Y

A

A

Q

Q

K

L

Q

V

G

G

V

V

K

E

R

R

I

F

V

V

Y

Y

A

S

S

S

S
|
S

N

G

H
x
C

A
x
G

V

V

-

Y

G

G

F

L

H

D

P

S

V

K

T

I

E

P

V

F

V

E

D

E

I

H

D

D

A

I

P

S

L

I

R

S

P

L

D

H

S

T

L

P

Y
|
Y

G

Q

V

V

T

T

K
|
K

C

L

F

L

G

G

E

E

S

L

L

Y

S

T

R

N

Y

Y

Y

F

F

H

D

N

R

L

F

Y

G

E

L

M

E

P

T

I

V

V

C

N

L

A

R

R

I

F

G

F

S
x
N

S

V

F

F

active site: S127 (= S123), C129 (≠ H125), G130 (≠ A126), Y153 (= Y148), K157 (= K152)
binding adenosine monophosphate: G16 (= G22), G19 (= G25), D40 (≠ T45), N41 (≠ D46), S43 (≠ A48), S44 (≠ P49), S45 (≠ L50), D62 (= D63), I63 (= I64), A84 (≠ G84), F87 (vs. gap)
binding uridine-5'-diphosphate: C129 (≠ H125), N182 (≠ S177)

Sites not aligning to the query:

binding adenosine monophosphate: 194
binding uridine-5'-diphosphate: 195, 196, 200, 211, 212, 213, 220, 256, 279

2q1wA Crystal structure of the bordetella bronchiseptica enzyme wbmh in complex with NAD+ (see paper)
29% identity, 58% coverage: 15:177/280 of query aligns to 1:168/300 of 2q1wA

query
sites
2q1wA

F

M

R

K

L

V

L

F

L

I

T

T

G
|
G

A

I

A

C

G
|
G

N
x
Q

L
x
I

G

G

K

S

Q

H

L

I

R

A

G

E

A

L

L

L

A

L

A

E

W

R

A

G

D

D

I

K

V

V

R

V

I

G

T

I

D
|
D

I
x
N

A
x
F

P
x
A

L
x
T

G
|
G

-

R

-

E

E

H

V

L

A

K

A

D

H

H

E

P

E

N

A

L

S

T

I

F

V

V

D

E

-

G

-
x
S

I
|
I

A

A

D

D

E

H

A

A

A
x
L

V

V

H

N

A

Q

L

L

L

I

E

G

G

D

V

L

-

Q

-

P

D

D

A

A

V

V

H

H

L
x
T

G
x
A

I

-

S

S

V
x
Y

E

K

A

D

P

P

-

D

-

W

F

Y

E

N

D

D

L

T

L

L

Q

-

A
x
T

N

N

I

C

R

V

G

G

L

G

Y

S

N

N

L

V

Y

V

S

Q

A

A

Q

K

K

K

Q

N

G

N

V

V

K

G

R

R

I

F

V

V

Y

Y

A
x
F

S

Q

S
x
T

N

A

H

L

A

C

V

Y

G

G

F

V

H

K

P

P

V

I

T

Q

E

Q

V

P

V

V

D

R

I

L

D

D

A

H

P

P

L

R

R

N

P

P

-

A

D

N

S

S

L

S

Y
|
Y

G

A

V

I

T

S

K
|
K

C

S

F

A

G

N

E

E

S

D

L

-

S

-

R

-

Y

-

Y

Y

F

L

D

E

R

Y

F

S

G

G

L

L

E

D

T

F

V

V

C

T

L

F

R

R

I

L

G

A

S
x
N

active site: S54 (vs. gap), L60 (≠ A69), T117 (≠ S123), Y143 (= Y148), K147 (= K152)
binding nicotinamide-adenine-dinucleotide: G8 (= G22), G11 (= G25), Q12 (≠ N26), I13 (≠ L27), D32 (= D46), N33 (≠ I47), F34 (≠ A48), A35 (≠ P49), T36 (≠ L50), G37 (= G51), S54 (vs. gap), I55 (= I64), T76 (≠ L83), A77 (≠ G84), A78 (≠ G85), Y80 (≠ V88), T92 (≠ A98), F115 (≠ A121), Y143 (= Y148), K147 (= K152), N168 (≠ S177)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 169

5u4qA 1.5 angstrom resolution crystal structure of NAD-dependent epimerase from klebsiella pneumoniae in complex with NAD.
26% identity, 61% coverage: 17:187/280 of query aligns to 3:184/321 of 5u4qA

query
sites
5u4qA

R

K

L

F

L

L

L

V

T

T

G
|
G

A

A

G
|
G

N
x
F

L
x
I

G

G

K

F

Q

H

L

I

-

A

R

Q

G

R

A

L

L

L

A

N

A

E

W

G

A

H

D

N

I

V

V

V

R

G

I

I

T
x
D

D
x
N

I

M

A
x
N

P

D

L

Y

G
x
Y

E

D

V

V

A

S

A

L

H
x
K

E

Q

-

A

-

R

-

L

-

D

-

R

-

L

-

A

-

Y

-

P

-

A

-

F

E

H

A

F

S

Q

I

Q

V

L

D
|
D

I
x
L

A

A

D

D

E

R

A

E

A

G

V

M

H

A

A

K

L

L

L

F

-

A

-

T

E

E

G

Q

V

F

D

D

A

R

V

V

V

I

H

H

L
|
L

G
x
A

I

Q

S

A

V

N

E

P

A

Y

P

A

F

Y

E

A

D

D

L

-

Q

-

A

A

N

N

I

L

R

M

G

G

L

Y

Y

L

N

N

L

I

Y

L

S

E

A

G

A

C

Q

R

K

H

Q

T

G

K

V

V

K

K

R

H

I

L

V

V

Y

Y

A
|
A

S

S

N

S

H

S

A

V

V

Y

G

G

F

L

H

N

P

R

V

K

T

M

E

P

V

F

V

S

D

T

I

E

D

D

A

S

P

V

L

D

R

H

P

P

D

V

S

S

L

L

Y
|
Y

G

A

V

A

T

T

K
|
K

C

K

F

A

G

N

E

E

S

L

L

M

S

A

R

H

Y

T

Y

Y

F

S

D

H

R

L

F

Y

G

G

L

I

E

P

T

T

V

T

C

G

L

L

R

R

I
x
F

G
x
F

S
x
T

S
x
V

F

Y

E

G

Q

P

P

W

K

G

N
x
R

P

P

R

D

M

M

binding nicotinamide-adenine-dinucleotide: G8 (= G22), G11 (= G25), F12 (≠ N26), I13 (≠ L27), D32 (≠ T45), N33 (≠ D46), N35 (≠ A48), Y38 (≠ G51), K43 (≠ H56), D61 (= D63), L62 (≠ I64), L83 (= L83), A84 (≠ G84), A85 (≠ G85), A118 (= A121), Y145 (= Y148), K149 (= K152), F172 (≠ I175), F173 (≠ G176), T174 (≠ S177), V175 (≠ S178), R181 (≠ N184)

Query Sequence

>BPHYT_RS24165 FitnessBrowser__BFirm:BPHYT_RS24165
MTDSNLDRSAAQKPFRRLLLTGAAGNLGKQLRGALAAWADIVRITDIAPLGEVAAHEEAS
IVDIADEAAVHALLEGVDAVVHLGGISVEAPFEDLLQANIRGLYNLYSAAQKQGVKRIVY
ASSNHAVGFHPVTEVVDIDAPLRPDSLYGVTKCFGESLSRYYFDRFGLETVCLRIGSSFE
QPKNPRMLVTYLSFRDLIELVRCSLFTNRVGHAIVYGVSDNRTKWVDNTKAAFLGFRPQD
SSVEFEHLFPVTAPTADLDDPTQRFQGGPFVLAGPMEPKR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory