SitesBLAST
Comparing BPHYT_RS24535 FitnessBrowser__BFirm:BPHYT_RS24535 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
2vpyF Polysulfide reductase with bound quinone inhibitor, pentachlorophenol (pcp) (see paper)
45% identity, 80% coverage: 1:200/251 of query aligns to 1:178/193 of 2vpyF
- binding pentachlorophenol: I91 (= I107), C93 (= C109)
- binding iron/sulfur cluster: C13 (= C13), G15 (= G15), C16 (= C16), C19 (= C19), C23 (= C23), N27 (= N27), L36 (≠ N51), I38 (≠ V53), P55 (= P70), Q57 (≠ S72), C58 (= C73), L59 (= L74), C61 (= C76), P65 (= P80), C66 (= C81), C70 (= C85), P71 (= P86), V83 (= V99), C90 (= C106), I91 (= I107), A92 (≠ G108), C93 (= C109), G94 (≠ K110), C96 (= C112), C100 (= C116), P101 (= P117), Y102 (= Y118), R105 (= R121), V113 (≠ M130), C116 (= C133), F118 (≠ L135), C119 (= C136), P129 (= P152), A130 (= A153), C131 (= C154), C135 (= C158), C139 (≠ A162), R140 (= R163)
Sites not aligning to the query:
2vpwF Polysulfide reductase with bound menaquinone (see paper)
45% identity, 80% coverage: 1:200/251 of query aligns to 1:178/193 of 2vpwF
- binding iron/sulfur cluster: C13 (= C13), G15 (= G15), C16 (= C16), C19 (= C19), C23 (= C23), N27 (= N27), L36 (≠ N51), I38 (≠ V53), P55 (= P70), Q57 (≠ S72), C58 (= C73), L59 (= L74), H60 (= H75), C61 (= C76), P65 (= P80), C66 (= C81), C70 (= C85), P71 (= P86), V83 (= V99), C90 (= C106), I91 (= I107), A92 (≠ G108), C93 (= C109), G94 (≠ K110), C96 (= C112), C100 (= C116), P101 (= P117), Y102 (= Y118), R105 (= R121), V113 (≠ M130), C116 (= C133), F118 (≠ L135), C119 (= C136), P129 (= P152), A130 (= A153), C131 (= C154), C135 (= C158), C139 (≠ A162), R140 (= R163)
Sites not aligning to the query:
6cz7B The arsenate respiratory reductase (arr) complex from shewanella sp. Ana-3 (see paper)
32% identity, 79% coverage: 3:200/251 of query aligns to 2:225/234 of 6cz7B
- binding iron/sulfur cluster: C12 (= C13), V13 (= V14), G14 (= G15), C15 (= C16), G16 (≠ H17), C18 (= C19), C22 (= C23), N26 (= N27), Y52 (≠ H68), P54 (= P70), C57 (= C73), N58 (≠ L74), H59 (= H75), C60 (= C76), A63 (≠ P79), P64 (= P80), C65 (= C81), C69 (= C85), P70 (= P86), T82 (≠ V99), C89 (= C106), I90 (= I107), G91 (= G108), C92 (= C109), K93 (= K110), C95 (= C112), C99 (= C116), Y101 (= Y118), V103 (≠ A120), I104 (vs. gap), T161 (≠ M130), K163 (= K132), C164 (= C133), F166 (≠ L135), C167 (= C136), C179 (= C154), C183 (= C158), P184 (= P159), R188 (= R163)
Q7WTT9 Arsenate respiratory reductase iron-sulfur subunit ArrB; Arsenate respiratory reductase small subunit; ARR small subunit from Shewanella sp. (strain ANA-3) (see paper)
32% identity, 79% coverage: 3:200/251 of query aligns to 2:225/234 of Q7WTT9
- C12 (= C13) binding
- C15 (= C16) binding
- C18 (= C19) binding
- C22 (= C23) binding
- C57 (= C73) binding
- C60 (= C76) binding
- C65 (= C81) binding
- C69 (= C85) binding
- C89 (= C106) binding
- C92 (= C109) binding
- C95 (= C112) binding
- C99 (= C116) binding
- C164 (= C133) binding
- C167 (= C136) binding
- C179 (= C154) binding
- C183 (= C158) binding
P18776 Anaerobic dimethyl sulfoxide reductase chain B; DMSO reductase iron-sulfur subunit from Escherichia coli (strain K12) (see 2 papers)
39% identity, 67% coverage: 2:168/251 of query aligns to 3:155/205 of P18776
- C102 (= C109) mutation C->F,S,W,Y: Loss of electron transfer from menaquinol to DMSO.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
6f0kB Alternative complex iii (see paper)
30% identity, 80% coverage: 2:203/251 of query aligns to 722:951/961 of 6f0kB
- binding fe3-s4 cluster: C803 (= C85), V805 (≠ T87), M818 (≠ V101), C823 (= C106), I824 (= I107), G825 (= G108), C829 (= C112), M869 (= M130)
- binding heme c: N821 (≠ D104), R822 (= R105), R884 (vs. gap), N887 (= N144)
- binding iron/sulfur cluster: C733 (= C13), T734 (≠ V14), G735 (= G15), C736 (= C16), N737 (≠ H17), A738 (= A18), C739 (= C19), C743 (= C23), W765 (≠ P40), I768 (≠ A43), C791 (= C73), M792 (≠ L74), H793 (= H75), C794 (= C76), P798 (= P80), C799 (= C81), N816 (≠ V99), C833 (= C116), C872 (= C133), Y874 (≠ L135), C875 (= C136), A901 (= A153), C902 (= C154), C906 (= C158)
Sites not aligning to the query:
P0AAJ3 Formate dehydrogenase, nitrate-inducible, iron-sulfur subunit; Anaerobic formate dehydrogenase iron-sulfur subunit; Formate dehydrogenase-N subunit beta; FDH-N subunit beta from Escherichia coli (strain K12) (see paper)
35% identity, 65% coverage: 3:166/251 of query aligns to 29:187/294 of P0AAJ3
- C39 (= C13) binding
- C42 (= C16) binding
- C45 (= C19) binding
- C49 (= C23) binding
- C100 (= C73) binding
- C103 (= C76) binding
- C108 (= C81) binding
- C112 (= C85) binding
- C133 (= C106) binding
- C136 (= C109) binding
- C139 (= C112) binding
- C143 (= C116) binding
- C160 (= C133) binding
- C163 (= C136) binding
- C175 (= C154) binding
- C179 (= C158) binding
1kqfB Formate dehydrogenase n from e. Coli (see paper)
35% identity, 65% coverage: 3:166/251 of query aligns to 28:186/289 of 1kqfB
- binding protoporphyrin ix containing fe: Y137 (= Y111)
- binding iron/sulfur cluster: K31 (≠ L6), C38 (= C13), I39 (≠ V14), G40 (= G15), C41 (= C16), K42 (≠ H17), C44 (= C19), C48 (= C23), N52 (= N27), T77 (≠ N51), M79 (≠ V53), C99 (= C73), M100 (≠ L74), H101 (= H75), C102 (= C76), P105 (= P79), C107 (= C81), C111 (= C85), P112 (= P86), I117 (≠ Y91), V125 (= V99), C132 (= C106), I133 (= I107), G134 (= G108), C135 (= C109), G136 (≠ K110), Y137 (= Y111), C138 (= C112), C142 (= C116), I146 (≠ A120), P147 (≠ R121), V156 (≠ M130), K158 (= K132), C159 (= C133), L161 (= L135), C162 (= C136), P172 (= P152), C174 (= C154), C178 (= C158), P179 (= P159), I183 (≠ R163)
Sites not aligning to the query:
5ch7B Crystal structure of the perchlorate reductase pcrab - phe164 gate switch intermediate - from azospira suillum ps (see paper)
27% identity, 94% coverage: 3:238/251 of query aligns to 7:282/329 of 5ch7B
- binding fe3-s4 cluster: C145 (= C85), I150 (≠ S90), C166 (= C106), K167 (≠ I107), G168 (= G108), A169 (≠ C109), Q170 (≠ K110), A171 (≠ Y111), C172 (= C112), A190 (≠ M130)
- binding iron/sulfur cluster: C17 (= C13), I18 (≠ V14), G19 (= G15), C20 (= C16), Q21 (≠ H17), C23 (= C19), C27 (= C23), W31 (vs. gap), R43 (vs. gap), V45 (vs. gap), P130 (= P70), C133 (= C73), N134 (≠ L74), C136 (= C76), P139 (= P79), C141 (= C81), C176 (= C116), C193 (= C133), G195 (≠ L135), C196 (= C136), C208 (= C154), C212 (= C158), V213 (≠ P159), G214 (≠ T160)
2ivfB Ethylbenzene dehydrogenase from aromatoleum aromaticum (see paper)
36% identity, 65% coverage: 50:211/251 of query aligns to 113:267/337 of 2ivfB
- binding fe3-s4 cluster: C148 (= C85), T150 (= T87), I153 (≠ S90), C169 (= C106), K170 (≠ I107), G171 (= G108), H172 (≠ C109), R173 (≠ K110), H174 (≠ Y111), C175 (= C112), S193 (≠ M130)
- binding protoporphyrin ix containing fe: T150 (= T87), K170 (≠ I107), H172 (≠ C109)
- binding iron/sulfur cluster: M135 (≠ S72), C136 (= C73), N137 (≠ L74), H138 (= H75), C139 (= C76), P142 (= P79), C144 (= C81), V162 (= V99), C179 (= C116), A183 (= A120), I184 (≠ R121), K195 (= K132), C196 (= C133), I197 (≠ T134), L198 (= L135), C199 (= C136), N209 (≠ P152), C211 (= C154), C215 (= C158), V219 (≠ A162), R220 (= R163)
Sites not aligning to the query:
- binding iron/sulfur cluster: 13, 14, 15, 16, 17, 19, 23, 27, 38, 39, 41
6lodB Cryo-em structure of the air-oxidized photosynthetic alternative complex iii from roseiflexus castenholzii (see paper)
28% identity, 80% coverage: 3:203/251 of query aligns to 682:920/929 of 6lodB
- binding fe3-s4 cluster: V758 (= V84), C759 (= C85), P760 (= P86), C779 (= C106), V780 (≠ I107), G781 (= G108), T782 (≠ C109), C785 (= C112), M838 (= M130)
- binding heme c: N777 (≠ D104), R778 (= R105), R852 (≠ N144), I853 (≠ L145), R856 (= R148)
- binding iron/sulfur cluster: C692 (= C13), N693 (≠ V14), S694 (≠ G15), C695 (= C16), N696 (≠ H17), A697 (= A18), C698 (= C19), C702 (= C23), N706 (= N27), W724 (≠ F50), I727 (≠ V53), L746 (≠ S72), C747 (= C73), Q748 (≠ L74), Q749 (≠ H75), C750 (= C76), P754 (= P80), C755 (= C81), N772 (≠ V99), C789 (= C116), Y791 (= Y118), V793 (≠ A120), R794 (= R121), K840 (= K132), C841 (= C133), F843 (≠ L135), C844 (= C136), T869 (≠ P152), C871 (= C154), C875 (= C158), I880 (≠ R163)
4v4cB Pyrogallol hydroxytransferase small subunit (see paper)
30% identity, 79% coverage: 1:199/251 of query aligns to 1:188/274 of 4v4cB
- binding calcium ion: I61 (≠ T66), N62 (≠ I67)
- binding iron/sulfur cluster: C13 (= C13), Q14 (≠ V14), D15 (≠ G15), C16 (= C16), N17 (≠ H17), N18 (≠ A18), C19 (= C19), C23 (= C23), C68 (= C73), M69 (≠ L74), H70 (= H75), C71 (= C76), C76 (= C81), V92 (= V99), C109 (= C116), V113 (≠ A120), C126 (= C133), M128 (≠ L135), C129 (= C136), P143 (= P152), C145 (= C154), C149 (= C158), S151 (≠ T160), V153 (≠ A162), Y154 (≠ R163)
3egwB The crystal structure of the narghi mutant narh - c16a
40% identity, 36% coverage: 50:140/251 of query aligns to 161:251/509 of 3egwB
- binding fe3-s4 cluster: P181 (= P70), C196 (= C85), S198 (≠ T87), I201 (≠ S90), C217 (= C106), R218 (≠ I107), G219 (= G108), W220 (≠ C109), R221 (≠ K110), C223 (= C112), S241 (≠ M130)
- binding protoporphyrin ix containing fe: W220 (≠ C109), R221 (≠ K110)
- binding iron/sulfur cluster: C184 (= C73), E185 (≠ L74), H186 (= H75), C187 (= C76), P190 (= P79), C192 (= C81), C227 (= C116), I232 (≠ R121), K243 (= K132), C244 (= C133), F246 (≠ L135), C247 (= C136)
Sites not aligning to the query:
- binding fe3-s4 cluster: 17, 18, 19, 20, 22, 263, 265, 268
- binding protoporphyrin ix containing fe: 88, 89
- binding iron/sulfur cluster: 26, 30, 41, 42, 257, 259
P11349 Respiratory nitrate reductase 1 beta chain; Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta; EC 1.7.5.1 from Escherichia coli (strain K12) (see 2 papers)
40% identity, 36% coverage: 50:140/251 of query aligns to 161:251/512 of P11349
- C184 (= C73) binding
- C187 (= C76) binding
- C192 (= C81) binding
- C196 (= C85) binding
- C217 (= C106) binding
- C223 (= C112) binding
- C227 (= C116) binding
- C244 (= C133) binding
- C247 (= C136) binding
Sites not aligning to the query:
- 16 binding
- 19 binding
- 22 binding
- 26 binding
- 259 binding
- 263 binding
6btmB Structure of alternative complex iii from flavobacterium johnsoniae (wild type) (see paper)
27% identity, 79% coverage: 6:203/251 of query aligns to 686:946/948 of 6btmB
- binding fe3-s4 cluster: C789 (= C85), C809 (= C106), V810 (≠ I107), T812 (≠ C109), C815 (= C112), M862 (= M130)
- binding iron/sulfur cluster: C777 (= C73), Q778 (≠ L74), C780 (= C76), C785 (= C81), N802 (≠ V99), C819 (= C116), R824 (= R121)
Sites not aligning to the query:
7b04A of Nitrite oxidoreductase (Nxr) from the anammox bacterium Kuenenia stuttgartiensis.
34% identity, 54% coverage: 67:201/251 of query aligns to 168:306/409 of 7b04A
- binding fe3-s4 cluster: C186 (= C85), I191 (≠ S90), C207 (= C106), R208 (≠ I107), G209 (= G108), Y210 (≠ C109), R211 (≠ K110), K212 (≠ Y111), C213 (= C112), S231 (≠ M130)
- binding protoporphyrin ix containing fe: R188 (≠ T87), R208 (≠ I107), Y210 (≠ C109)
- binding iron/sulfur cluster: Q171 (≠ P70), I173 (≠ S72), C174 (= C73), N175 (≠ L74), H176 (= H75), C177 (= C76), P180 (= P79), C182 (= C81), C217 (= C116), P222 (≠ R121), K233 (= K132), C234 (= C133), A236 (≠ L135), C237 (= C136), T255 (≠ P152), R256 (≠ A153), C257 (= C154), C261 (= C158), V262 (≠ P159), I265 (≠ A162), R266 (= R163)
Sites not aligning to the query:
- binding iron/sulfur cluster: 34, 35, 36, 37, 38, 40, 44, 48, 59, 60, 62
8cm6D W-formate dehydrogenase c872a from desulfovibrio vulgaris - with formamide (see paper)
30% identity, 70% coverage: 6:181/251 of query aligns to 4:179/214 of 8cm6D
- binding formamide: R59 (vs. gap), P62 (= P62)
- binding iron/sulfur cluster: F4 (≠ L6), C11 (= C13), T12 (≠ V14), A13 (≠ G15), C14 (= C16), R15 (≠ H17), C17 (= C19), C21 (= C23), K25 (vs. gap), K50 (≠ N51), C73 (= C73), R74 (≠ L74), C76 (= C76), P79 (= P79), P80 (= P80), C81 (= C81), V102 (= V99), C120 (= C116), I124 (≠ A120), P125 (≠ R121), K136 (= K132), C137 (= C133), D138 (≠ T134), M139 (≠ L135), C140 (= C136), C152 (= C154), C156 (= C158), P157 (= P159), T158 (= T160), T160 (≠ A162), M161 (≠ R163)
8bqgB W-formate dehydrogenase from desulfovibrio vulgaris - soaking with formate 1 min (see paper)
30% identity, 70% coverage: 6:181/251 of query aligns to 4:179/214 of 8bqgB
- binding iron/sulfur cluster: F4 (≠ L6), C11 (= C13), T12 (≠ V14), A13 (≠ G15), C14 (= C16), R15 (≠ H17), C17 (= C19), C21 (= C23), K25 (vs. gap), K50 (≠ N51), C73 (= C73), R74 (≠ L74), C76 (= C76), P80 (= P80), C81 (= C81), V102 (= V99), C120 (= C116), I124 (≠ A120), P125 (≠ R121), K136 (= K132), C137 (= C133), M139 (≠ L135), C140 (= C136), C152 (= C154), C156 (= C158), P157 (= P159), T158 (= T160), T160 (≠ A162), M161 (≠ R163)
Q8GC87 Formate dehydrogenase subunit beta; FDH subunit beta; Formate dehydrogenase small subunit from Megalodesulfovibrio gigas (strain ATCC 19364 / DSM 1382 / NCIMB 9332 / VKM B-1759) (Desulfovibrio gigas) (see paper)
28% identity, 70% coverage: 6:181/251 of query aligns to 5:180/215 of Q8GC87
- C12 (= C13) binding
- C15 (= C16) binding
- C18 (= C19) binding
- C22 (= C23) binding
- C73 (= C73) binding
- C76 (= C76) binding
- C81 (= C81) binding
- C121 (= C116) binding
- C138 (= C133) binding
- C141 (= C136) binding
- C153 (= C154) binding
- C157 (= C158) binding
7qv7B Cryo-em structure of hydrogen-dependent co2 reductase. (see paper)
33% identity, 60% coverage: 13:162/251 of query aligns to 14:158/183 of 7qv7B
- binding iron/sulfur cluster: C14 (= C13), G16 (= G15), C17 (= C16), K18 (≠ H17), A19 (= A18), C20 (= C19), C24 (= C23), H28 (≠ W26), R48 (≠ S46), C63 (= C73), R64 (≠ L74), H65 (= H75), C66 (= C76), A69 (≠ P79), C71 (= C81), C75 (= C85), A79 (= A89), I80 (≠ S90), C94 (= C106), I95 (= I107), C97 (= C109), C100 (= C112), C104 (= C116), I109 (≠ R121), C139 (= C133), L141 (= L135), C142 (= C136), P148 (= P152), C150 (= C154), C154 (= C158), P155 (= P159)
Sites not aligning to the query:
Query Sequence
>BPHYT_RS24535 FitnessBrowser__BFirm:BPHYT_RS24535
MTQMALVIDLNVCVGCHACVTSCKEWNTSGESGSLADLNPYDADPSGTFFNRVQSFEAGS
FPNAETIHFPKSCLHCEDPPCVPVCPTGASYKRKEDGLVLVDFDRCIGCKYCAWACPYGA
RELDESRKEMTKCTLCVDRIHDENLSERDRQPACVLACPTSARLFGDIHDPESVVSKAIE
ERGGYQLMPEWNTRPANHYLPRVPTEAAGCGSGSCSCKSSGSLQEPSASPESLDAQFERG
ELHLASMATRI
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory