SitesBLAST
Comparing BPHYT_RS25050 FitnessBrowser__BFirm:BPHYT_RS25050 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3flkA Crystal structure of tartrate dehydrogenase from pseudomonas putida in complex with nadh, oxalate and metal ion (see paper)
48% identity, 94% coverage: 3:354/376 of query aligns to 1:357/359 of 3flkA
- active site: Y137 (= Y138), K188 (= K189), D221 (= D222), D245 (= D246), D249 (= D250)
- binding 1,4-dihydronicotinamide adenine dinucleotide: I12 (= I14), A73 (≠ S75), V74 (≠ A76), G75 (= G77), D82 (= D83), L90 (≠ R91), N190 (= N191), I222 (≠ A223), R226 (= R227), I258 (≠ L259), H280 (= H281), G281 (= G282), S282 (= S283), A283 (= A284), I286 (= I287), N293 (= N294)
- binding oxalate ion: R94 (≠ C95), R104 (= R105), R130 (= R131), D245 (= D246)
2g4oA Anomalous substructure of 3-isopropylmalate dehydrogenase (see paper)
42% identity, 85% coverage: 5:323/376 of query aligns to 3:313/337 of 2g4oA
4yb4A Crystal structure of homoisocitrate dehydrogenase from thermus thermophilus in complex with homoisocitrate, magnesium ion (ii) and nadh
39% identity, 94% coverage: 4:355/376 of query aligns to 2:332/333 of 4yb4A
- active site: Y124 (= Y138), K170 (= K189), D203 (= D222), D227 (= D246), D231 (= D250)
- binding (1R,2S)-1-hydroxybutane-1,2,4-tricarboxylic acid: S71 (≠ D78), R84 (≠ L92), R87 (≠ C95), R97 (= R105), R117 (= R131), Y124 (= Y138), D227 (= D246)
- binding 1,4-dihydronicotinamide adenine dinucleotide: I12 (= I14), A69 (= A76), T70 (≠ G77), S71 (≠ D78), I201 (≠ L220), N204 (≠ A223), L240 (= L259), E256 (= E278), H259 (= H281), G260 (= G282), S261 (= S283), A262 (= A284), D264 (= D286), I265 (= I287), N272 (= N294), D312 (= D335)
3asjB Crystal structure of homoisocitrate dehydrogenase in complex with a designed inhibitor (see paper)
39% identity, 94% coverage: 4:355/376 of query aligns to 2:332/333 of 3asjB
- active site: Y124 (= Y138), K170 (= K189), D203 (= D222), D227 (= D246), D231 (= D250)
- binding (2Z)-3-[(carboxymethyl)sulfanyl]-2-hydroxyprop-2-enoic acid: R84 (≠ L92), R97 (= R105), R117 (= R131), Y124 (= Y138), D227 (= D246), D231 (= D250), V258 (≠ I280)
3asjA Crystal structure of homoisocitrate dehydrogenase in complex with a designed inhibitor (see paper)
39% identity, 94% coverage: 4:355/376 of query aligns to 2:332/333 of 3asjA
Q72IW9 Isocitrate/homoisocitrate dehydrogenase; Homoisocitrate dehydrogenase; HICDH; EC 1.1.1.286 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see 4 papers)
39% identity, 94% coverage: 4:355/376 of query aligns to 3:333/334 of Q72IW9
- E57 (≠ D63) mutation to V: Confers enzyme activity with 3-isopropylmalate; when associated with I-72; M-85; A-86; T-208; Y-217; M-238 and M-310.
- ATS 70:72 (≠ AGD 76:78) binding
- S72 (≠ D78) binding in other chain; mutation to I: Confers enzyme activity with 3-isopropylmalate; when associated with V-57; M-85; A-86; T-208; Y-217; M-238 and M-310.
- R85 (≠ L92) binding in other chain; mutation to M: Confers enzyme activity with 3-isopropylmalate; when associated with V-57; I-72; A-86; T-208; Y-217; M-238 and M-310.; mutation to V: Confers low enzyme activity with 3-isopropylmalate. Reduces activity with homoisocitrate. Abolishes activity with isocitrate.
- Y86 (≠ K93) mutation to A: Confers enzyme activity with 3-isopropylmalate; when associated with V-57; I-72; M-85; T-208; Y-217; M-238 and M-310.
- R88 (≠ C95) binding in other chain
- R98 (= R105) binding in other chain
- R118 (= R131) binding in other chain
- Y125 (= Y138) binding in other chain; mutation to A: Reduces catalytic efficiency with isocitrate.
- V135 (= V153) mutation to M: Formation of homodimers instead of homotetramers. Increased affinity for isocitrate. Reduces enzyme activity with isocitrate.
- K171 (= K189) binding
- N173 (= N191) binding ; binding
- D204 (= D222) binding
- M208 (≠ A226) mutation to T: Confers enzyme activity with 3-isopropylmalate; when associated with V-57; I-72; M-85; A-86; T-208; Y-217; M-238 and M-310.
- F217 (≠ L235) mutation to Y: Confers enzyme activity with 3-isopropylmalate; when associated with V-57; I-72; M-85; A-86; T-208; M-238 and M-310.
- D228 (= D246) binding
- D232 (= D250) binding
- V238 (≠ A256) mutation to M: Confers enzyme activity with 3-isopropylmalate; when associated with V-57; I-72; M-85; A-86; T-208; Y-217; and M-310.
- GSAPD 261:265 (≠ GSAFD 282:286) binding
- N273 (= N294) binding
- R310 (≠ H332) mutation to M: Confers enzyme activity with 3-isopropylmalate; when associated with V-57; I-72; M-85; A-86; T-208; Y-217; and M-238.
P40495 Homoisocitrate dehydrogenase, mitochondrial; HIcDH; EC 1.1.1.87 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
36% identity, 94% coverage: 2:353/376 of query aligns to 22:371/371 of P40495
- Y150 (= Y138) mutation to F: Strongly reduced enzyme activity.
- K206 (= K189) mutation to M: Strongly reduced enzyme activity.
6lkyA Crystal structure of isocitrate dehydrogenase from methylococcus capsulatus
36% identity, 89% coverage: 4:336/376 of query aligns to 2:318/339 of 6lkyA
- active site: Y123 (= Y138), K174 (= K189), D207 (= D222), D231 (= D246)
- binding nicotinamide-adenine-dinucleotide: P68 (≠ D78), L69 (≠ P79), T71 (≠ I81), N81 (≠ R91), H263 (= H281), G264 (= G282), S265 (= S283), A266 (= A284), D268 (= D286), I269 (= I287), N276 (= N294)
2d1cA Crystal structure of tt0538 protein from thermus thermophilus hb8
36% identity, 96% coverage: 6:366/376 of query aligns to 21:364/495 of 2d1cA
- active site: Y143 (= Y138), K190 (= K189), D223 (= D222), D247 (= D246), D251 (= D250)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: P86 (≠ S75), L87 (≠ A76), E88 (≠ G77), T89 (≠ D78), N99 (≠ H84), I221 (≠ L220), N224 (≠ A223), Q228 (≠ R227), L260 (= L259), G261 (= G260), H279 (= H281), G280 (= G282), S281 (= S283), A282 (= A284), K284 (≠ D286), Y285 (≠ I287), I291 (≠ A293), N292 (= N294), D333 (= D335)
O14104 Homoisocitrate dehydrogenase; HICDH; EC 1.1.1.87 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
34% identity, 92% coverage: 6:351/376 of query aligns to 9:358/362 of O14104
- S81 (≠ D78) modified: Phosphoserine
- S91 (≠ T86) modified: Phosphoserine
3ty3A Crystal structure of homoisocitrate dehydrogenase from schizosaccharomyces pombe bound to glycyl-glycyl-glycine (see paper)
34% identity, 92% coverage: 6:351/376 of query aligns to 5:354/358 of 3ty3A
- active site: Y129 (= Y138), K192 (= K189), D228 (= D222), D252 (= D246), D256 (= D250)
- binding glycylglycylglycine: A74 (≠ S75), V75 (≠ A76), S77 (≠ D78), R93 (= R91), E281 (= E278), P282 (= P279), H284 (= H281)
6m3sB Dimeric isocitrate dehydrogenase from xanthomonas campestris pv. Campestris 8004
34% identity, 93% coverage: 3:353/376 of query aligns to 5:338/338 of 6m3sB
- active site: Y128 (= Y138), K177 (= K189), D210 (= D222), D234 (= D246)
- binding isocitrate calcium complex: T75 (≠ P79), S83 (≠ L87), N85 (≠ G89), R89 (≠ K93), R99 (= R105), R121 (= R131), Y128 (= Y138), D234 (= D246), D238 (= D250)
- binding nicotinamide-adenine-dinucleotide: P72 (≠ A76), L73 (≠ G77), T75 (≠ P79), N85 (≠ G89), H266 (= H281), G267 (= G282), S268 (= S283), A269 (= A284), D271 (= D286), I272 (= I287), N279 (= N294)
3blwF Yeast isocitrate dehydrogenase with citrate and amp bound in the regulatory subunits (see paper)
35% identity, 92% coverage: 4:349/376 of query aligns to 19:343/347 of 3blwF
2y41A Structure of isopropylmalate dehydrogenase from thermus thermophilus - complex with ipm and mn (see paper)
35% identity, 94% coverage: 3:354/376 of query aligns to 1:346/346 of 2y41A
2y42D Structure of isopropylmalate dehydrogenase from thermus thermophilus - complex with nadh and mn (see paper)
35% identity, 93% coverage: 3:350/376 of query aligns to 1:342/355 of 2y42D
- active site: Y140 (= Y138), K186 (= K189), D218 (= D222), D242 (= D246), D246 (= D250)
- binding manganese (ii) ion: D242 (= D246), D246 (= D250)
- binding nicotinamide-adenine-dinucleotide: I12 (= I14), D79 (vs. gap), H274 (= H281), G275 (= G282), A277 (= A284), D279 (= D286), I280 (= I287), N287 (= N294)
2ztwA Structure of 3-isopropylmalate dehydrogenase in complex with the inhibitor and NAD+ (see paper)
35% identity, 93% coverage: 5:354/376 of query aligns to 2:345/345 of 2ztwA
- active site: Y139 (= Y138), K185 (= K189), D217 (= D222), D241 (= D246), D245 (= D250)
- binding magnesium ion: G203 (≠ S208), Y206 (≠ F211), V209 (= V214)
- binding nicotinamide-adenine-dinucleotide: I11 (= I14), H273 (= H281), G274 (= G282), A276 (= A284), D278 (= D286), I279 (= I287), A285 (= A293), N286 (= N294)
Q5SIY4 3-isopropylmalate dehydrogenase; 3-IPM-DH; Beta-IPM dehydrogenase; IMDH; EC 1.1.1.85 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see 2 papers)
35% identity, 93% coverage: 5:354/376 of query aligns to 2:345/345 of Q5SIY4
- 74:87 (vs. 78:88, 14% identical) binding
- Y139 (= Y138) mutation to F: Large decrease in activity and a small decrease in substrate affinity.
- 274:286 (vs. 282:294, 77% identical) binding
5hn6A Crystal structure of beta-decarboxylating dehydrogenase (tk0280) from thermococcus kodakarensis complexed with mn and 3-isopropylmalate (see paper)
37% identity, 93% coverage: 4:351/376 of query aligns to 2:317/329 of 5hn6A
5hn4A Crystal structure of beta-decarboxylating dehydrogenase (tk0280) from thermococcus kodakarensis complexed with mn and homoisocitrate (see paper)
37% identity, 93% coverage: 4:351/376 of query aligns to 2:317/329 of 5hn4A
- active site: Y118 (= Y138), K163 (= K189), D194 (= D222), D218 (= D246), D222 (= D250)
- binding (1R,2S)-1-hydroxybutane-1,2,4-tricarboxylic acid: T71 (≠ D78), S80 (≠ T86), R86 (≠ C95), R96 (= R105), R111 (= R131), Y118 (= Y138), D218 (= D246)
4y1pB Crystal structure of 3-isopropylmalate dehydrogenase (saci_0600) from sulfolobus acidocaldarius complex with 3-isopropylmalate and mg2+ (see paper)
35% identity, 92% coverage: 4:349/376 of query aligns to 2:331/336 of 4y1pB
Query Sequence
>BPHYT_RS25050 FitnessBrowser__BFirm:BPHYT_RS25050
MKTYRIATIPGDGIGKEVVPAGRRVLEAIAGSGDAFGFEFENFDWGGDYYREHGVMMPAD
GLDAIRHKDAILFGSAGDPDIPDHVTLWGLRLKICQGFDQYANVRPTRILPGIDAPLKRC
GPEDLDWVIVRENSEGEYAGVGGRAHQGHPIEVATDVSMMTRAGVERILRFAFQLAQSRP
RKLLTVITKSNAQRHAMVMWDEIAVQVSKEFPDVRWDKELVDAATARMVNRPATLDTIVA
TNLHADILSDLAAALAGSLGIAPTANLDPERRYPSMFEPIHGSAFDIMGKGLANPVGTFW
SVVMMLEHLGEHAAAQQLMAAIERVTADPSLHTGDLGGTATTARVTDAVCAILAAASPTD
ASTSARAEPVASVKAA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory