SitesBLAST
Comparing BPHYT_RS28015 FitnessBrowser__BFirm:BPHYT_RS28015 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3bptA Crystal structure of human beta-hydroxyisobutyryl-coa hydrolase in complex with quercetin
35% identity, 93% coverage: 14:370/382 of query aligns to 2:352/362 of 3bptA
- active site: G67 (= G79), P84 (≠ L96), R88 (≠ V100), G115 (= G127), G118 (= G130), E138 (= E150), D146 (= D158)
- binding (2r)-3-hydroxy-2-methylpropanoic acid: G66 (= G78), G67 (= G79), I69 (≠ V81), E90 (= E102), G114 (= G126), G115 (= G127), E138 (= E150), D146 (= D158), V147 (= V159)
- binding 3,5,7,3',4'-pentahydroxyflavone: F25 (≠ A37), L26 (= L38), A28 (= A40), G66 (= G78), G67 (= G79), I69 (≠ V81), P137 (= P149), I141 (= I153), L319 (= L337)
Q9LKJ1 3-hydroxyisobutyryl-CoA hydrolase 1; CoA-thioester hydrolase CHY1; EC 3.1.2.-; EC 3.1.2.4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
35% identity, 94% coverage: 13:370/382 of query aligns to 5:360/378 of Q9LKJ1
- G70 (= G79) mutation to S: Loss of activity.
- E142 (= E150) mutation to A: Loss of activity.
- D150 (= D158) mutation to G: Reduced activity.
4hdtA Crystal structure of a carnitinyl-coa dehydratase from mycobacterium thermoresistibile (see paper)
36% identity, 91% coverage: 16:364/382 of query aligns to 1:329/340 of 4hdtA
- active site: G64 (= G79), I69 (≠ V84), W84 (≠ F99), Y88 (= Y103), G112 (= G127), G115 (= G130), E135 (= E150), P142 (= P157), D143 (= D158), R283 (= R314)
- binding zinc ion: H28 (= H43), E42 (≠ R57), E57 (≠ A72), E79 (≠ R94), H93 (≠ A108), H185 (≠ A200)
Sites not aligning to the query:
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
31% identity, 48% coverage: 30:213/382 of query aligns to 17:194/259 of 5zaiC
- active site: A65 (≠ G79), F70 (≠ V84), S82 (≠ F99), R86 (≠ Y103), G110 (= G127), E113 (≠ G130), P132 (= P149), E133 (= E150), I138 (≠ F155), P140 (= P157), G141 (≠ D158)
- binding coenzyme a: K24 (≠ A37), L25 (= L38), A63 (= A77), G64 (= G78), A65 (≠ G79), D66 (= D80), I67 (≠ V81), P132 (= P149), R166 (≠ T182)
Sites not aligning to the query:
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
34% identity, 49% coverage: 25:211/382 of query aligns to 15:194/260 of 2hw5C
- active site: A68 (≠ G79), M73 (≠ V84), S83 (≠ R104), L87 (≠ A108), G111 (= G127), E114 (≠ G130), P133 (= P149), E134 (= E150), T139 (≠ F155), P141 (= P157), G142 (≠ D158)
- binding crotonyl coenzyme a: K26 (≠ A36), A27 (= A37), L28 (= L38), A30 (= A40), K62 (= K73), I70 (≠ V81), F109 (≠ M125)
Sites not aligning to the query:
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
33% identity, 48% coverage: 27:211/382 of query aligns to 17:194/260 of 1dubA
- active site: A68 (≠ G79), M73 (≠ V84), S83 (≠ R104), L87 (≠ A108), G111 (= G127), E114 (≠ G130), P133 (= P149), E134 (= E150), T139 (≠ F155), P141 (= P157), G142 (≠ D158)
- binding acetoacetyl-coenzyme a: K26 (≠ A36), A27 (= A37), L28 (= L38), A30 (= A40), A66 (= A77), A68 (≠ G79), D69 (= D80), I70 (≠ V81), Y107 (≠ I123), G110 (= G126), G111 (= G127), E114 (≠ G130), P133 (= P149), E134 (= E150), L137 (≠ I153), G142 (≠ D158)
Sites not aligning to the query:
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
33% identity, 48% coverage: 27:211/382 of query aligns to 47:224/290 of P14604
- E144 (≠ G130) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (= E150) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
33% identity, 49% coverage: 25:211/382 of query aligns to 13:192/258 of 1ey3A
- active site: A66 (≠ G79), M71 (≠ V84), S81 (≠ R104), L85 (≠ A108), G109 (= G127), E112 (≠ G130), P131 (= P149), E132 (= E150), T137 (≠ F155), P139 (= P157), G140 (≠ D158)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ A36), L26 (= L38), A28 (= A40), A64 (= A77), G65 (= G78), A66 (≠ G79), D67 (= D80), I68 (≠ V81), L85 (≠ A108), W88 (vs. gap), G109 (= G127), P131 (= P149), L135 (≠ I153), G140 (≠ D158)
Sites not aligning to the query:
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
34% identity, 48% coverage: 27:211/382 of query aligns to 16:188/254 of 2dubA
- active site: A67 (≠ G79), M72 (≠ V84), S82 (= S93), G105 (= G127), E108 (≠ G130), P127 (= P149), E128 (= E150), T133 (≠ F155), P135 (= P157), G136 (≠ D158)
- binding octanoyl-coenzyme a: K25 (≠ A36), A26 (= A37), L27 (= L38), A29 (= A40), A65 (= A77), A67 (≠ G79), D68 (= D80), I69 (≠ V81), K70 (≠ R82), G105 (= G127), E108 (≠ G130), P127 (= P149), E128 (= E150), G136 (≠ D158), A137 (≠ V159)
Sites not aligning to the query:
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
32% identity, 48% coverage: 19:203/382 of query aligns to 5:184/256 of 3h81A
- active site: A64 (≠ G79), M69 (≠ V84), T79 (≠ R104), F83 (vs. gap), G107 (= G127), E110 (≠ G130), P129 (= P149), E130 (= E150), V135 (≠ F155), P137 (= P157), G138 (≠ D158)
Sites not aligning to the query:
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
32% identity, 48% coverage: 19:203/382 of query aligns to 6:185/255 of 3q0jC
- active site: A65 (≠ G79), M70 (≠ V84), T80 (≠ R104), F84 (vs. gap), G108 (= G127), E111 (≠ G130), P130 (= P149), E131 (= E150), V136 (≠ F155), P138 (= P157), G139 (≠ D158)
- binding acetoacetyl-coenzyme a: Q23 (≠ A36), A24 (= A37), L25 (= L38), A27 (= A40), A63 (= A77), G64 (= G78), A65 (≠ G79), D66 (= D80), I67 (≠ V81), K68 (≠ R82), M70 (≠ V84), F84 (vs. gap), G107 (= G126), G108 (= G127), E111 (≠ G130), P130 (= P149), E131 (= E150), P138 (= P157), G139 (≠ D158), M140 (≠ V159)
Sites not aligning to the query:
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
32% identity, 48% coverage: 19:203/382 of query aligns to 6:185/255 of 3q0gC
- active site: A65 (≠ G79), M70 (≠ V84), T80 (≠ R104), F84 (vs. gap), G108 (= G127), E111 (≠ G130), P130 (= P149), E131 (= E150), V136 (≠ F155), P138 (= P157), G139 (≠ D158)
- binding coenzyme a: L25 (= L38), A63 (= A77), I67 (≠ V81), K68 (≠ R82), Y104 (≠ I123), P130 (= P149), E131 (= E150), L134 (≠ I153)
Sites not aligning to the query:
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
34% identity, 48% coverage: 27:211/382 of query aligns to 17:192/258 of 1mj3A
- active site: A68 (≠ G79), M73 (≠ V84), S83 (≠ R104), L85 (= L105), G109 (= G127), E112 (≠ G130), P131 (= P149), E132 (= E150), T137 (≠ F155), P139 (= P157), G140 (≠ D158)
- binding hexanoyl-coenzyme a: K26 (≠ A36), A27 (= A37), L28 (= L38), A30 (= A40), A66 (= A77), G67 (= G78), A68 (≠ G79), D69 (= D80), I70 (≠ V81), G109 (= G127), P131 (= P149), E132 (= E150), L135 (≠ I153), G140 (≠ D158)
Sites not aligning to the query:
7xwtB Crystal structure of feruoyl-coa hydratase/lyase complexed with coa from sphingomonas paucimobilis (see paper)
30% identity, 50% coverage: 16:205/382 of query aligns to 5:195/277 of 7xwtB
- binding acetyl coenzyme *a: T25 (≠ A36), K26 (≠ A37), R27 (≠ L38), A29 (= A40), A65 (= A77), G66 (= G78), M67 (≠ G79), D68 (= D80), L69 (≠ V81), F73 (≠ Q85), F114 (≠ M125), G116 (= G127), S138 (≠ P149), W142 (≠ I153)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
32% identity, 48% coverage: 19:203/382 of query aligns to 5:180/250 of 3q0gD
- active site: A64 (≠ G79), M69 (≠ V84), T75 (≠ R104), F79 (vs. gap), G103 (= G127), E106 (≠ G130), P125 (= P149), E126 (= E150), V131 (≠ F155), P133 (= P157), G134 (≠ D158)
Sites not aligning to the query:
7xwvA Feruloyl-coa hydratase/lyase complexed with vanillin and coenzyme a (see paper)
30% identity, 49% coverage: 16:201/382 of query aligns to 4:190/244 of 7xwvA
- binding coenzyme a: T24 (≠ A36), K25 (≠ A37), R26 (≠ L38), A64 (= A77), G65 (= G78), M66 (≠ G79), D67 (= D80), L68 (≠ V81), W111 (≠ I123), F113 (≠ M125), G114 (= G126), G115 (= G127), S137 (≠ P149)
- binding 4-hydroxy-3-methoxybenzaldehyde: M66 (≠ G79), Y71 (≠ V84), F72 (≠ Q85), E138 (= E150), G146 (≠ D158), G147 (≠ V159)
Q08426 Peroxisomal bifunctional enzyme; PBE; PBFE; L-bifunctional protein; LBP; Multifunctional enzyme 1; MFE1; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 from Homo sapiens (Human) (see 5 papers)
32% identity, 51% coverage: 22:216/382 of query aligns to 6:194/723 of Q08426
- V40 (≠ R57) to G: in dbSNP:rs1062551
- I41 (≠ T58) to R: in dbSNP:rs1062552
- T75 (≠ W95) to I: in dbSNP:rs1062553
- K165 (≠ R190) modified: N6-acetyllysine; alternate; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-171; Q-346 and Q-584.
- K171 (≠ L196) modified: N6-acetyllysine; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-346 and Q-584.
Sites not aligning to the query:
- 3 E → K: in FRTS3; the mutant is mistargeted to mitochondria; results in impaired mitochondrial oxidative phosphorylation and defects in the transport of fluids across the epithelium of renal proximal tubular cells; dbSNP:rs398124646
- 274 A → T: in dbSNP:rs2302819
- 325 A → G: in dbSNP:rs1062555
- 346 modified: N6-acetyllysine; K→Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-584.
- 584 modified: N6-acetyllysine; alternate; K→Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-346.
- 598 K → T: in dbSNP:rs1042437
- 606 T → P: in dbSNP:rs1042438
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
29% identity, 58% coverage: 21:243/382 of query aligns to 7:210/261 of 5jbxB
- active site: A67 (≠ G79), R72 (≠ V84), L84 (≠ V100), R88 (= R104), G112 (= G127), E115 (≠ G130), T134 (≠ P149), E135 (= E150), I140 (≠ F155), P142 (= P157), G143 (≠ D158)
- binding coenzyme a: S24 (≠ A36), R25 (≠ A37), R26 (≠ L38), A28 (= A40), A65 (= A77), D68 (= D80), L69 (≠ V81), K70 (≠ R82), L110 (≠ M125), G111 (= G126), T134 (≠ P149), E135 (= E150), L138 (≠ I153), R168 (≠ T182)
Sites not aligning to the query:
6eqoA Tri-functional propionyl-coa synthase of erythrobacter sp. Nap1 with bound NADP+ and phosphomethylphosphonic acid adenylate ester (see paper)
30% identity, 46% coverage: 25:201/382 of query aligns to 865:1032/1804 of 6eqoA
Sites not aligning to the query:
- binding phosphomethylphosphonic acid adenylate ester: 456, 458, 535, 536, 537, 538, 558, 559, 560, 561, 562, 688, 714
- binding nadp nicotinamide-adenine-dinucleotide phosphate: 1261, 1265, 1379, 1400, 1403, 1404, 1405, 1424, 1425, 1429, 1444, 1492, 1493, 1497, 1514, 1517, 1713, 1730, 1731, 1774
4izdA Crystal structure of dmdd e121a in complex with mmpa-coa (see paper)
31% identity, 49% coverage: 25:211/382 of query aligns to 18:199/253 of 4izdA
- active site: L70 (≠ G79), H75 (≠ V84), C89 (vs. gap), H93 (≠ L96), G117 (= G127), A120 (≠ G130), E140 (= E150), G148 (≠ D158)
- binding 3-methylmercaptopropionate-CoA (MMPA-CoA): D29 (≠ A36), K30 (≠ A37), R31 (≠ L38), A33 (= A40), A68 (= A77), L70 (≠ G79), D71 (= D80), L72 (≠ V81)
Sites not aligning to the query:
Query Sequence
>BPHYT_RS28015 FitnessBrowser__BFirm:BPHYT_RS28015
MSALQSVAHDTSLEAEREILFRVVNRVAIITLNRPAALNALSHAMVRELAVLVEHCRTDD
GIVALVLKGAGAKGFCAGGDVREVQRLAKNNDSRWLAFFVDEYRLDYALHTFPKPVVALL
DGIAMGGGMGLGQGARLRIVTERSKIAMPETRIGFLPDVGATRFLSVMPAELELYVGLTG
ATLSGADALRLQLADLCVPAEWLASFEERLQRMPLEGDLMAALRGVFEPPCNIIPHAPLA
AFTQLILRHFDRRSSIDRMVATVRHDLLREPPREVKQWLQSTYDALTGHSPTMLYVTREA
LLRGRQMTLAECFRMELGIVKRVIEEGDFCEGVRAQLIDKDRKARWAPATLAEVRPERVR
HFLASPWKSDAHPLAALGAEQG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory