SitesBLAST
Comparing BPHYT_RS28150 BPHYT_RS28150 methylmalonate-semialdehyde dehydrogenase to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4zz7A Crystal structure of methylmalonate-semialdehyde dehydrogenase (dddc) from oceanimonas doudoroffii (see paper)
56% identity, 95% coverage: 12:492/505 of query aligns to 4:484/489 of 4zz7A
- active site: N149 (= N158), K172 (= K181), L246 (= L255), C280 (= C289), E382 (= E390), A462 (= A470)
- binding nicotinamide-adenine-dinucleotide: T146 (= T155), P147 (= P156), F148 (= F157), N149 (= N158), K172 (= K181), E175 (= E184), K205 (= K214), V208 (= V217), F222 (≠ V231), V223 (= V232), G224 (= G233), S225 (= S234), I228 (≠ V237), L246 (= L255), G247 (= G256), C280 (= C289), E382 (= E390), F384 (= F392)
5tjrD X-ray crystal structure of a methylmalonate semialdehyde dehydrogenase from pseudomonas sp. Aac (see paper)
57% identity, 97% coverage: 12:501/505 of query aligns to 3:468/468 of 5tjrD
- active site: N144 (= N158), K167 (= K181), L241 (= L255), C270 (= C289), E356 (= E390), A436 (= A470)
- binding adenosine-5'-diphosphate: I140 (≠ V154), T141 (= T155), F143 (= F157), K167 (= K181), E170 (= E184), K200 (= K214), F217 (≠ V231), S220 (= S234), I223 (≠ V237)
4iymC Crystal structure of putative methylmalonate-semialdehyde dehydrogenase from sinorhizobium meliloti 1021 complexed with NAD, target 011934
54% identity, 97% coverage: 6:493/505 of query aligns to 1:489/491 of 4iymC
- active site: N153 (= N158), K176 (= K181), F250 (≠ L255), C284 (= C289), E386 (= E390), Q466 (≠ A470)
- binding nicotinamide-adenine-dinucleotide: I149 (≠ V154), T150 (= T155), P151 (= P156), F152 (= F157), N153 (= N158), F154 (= F159), K176 (= K181), K209 (= K214), V212 (= V217), F226 (≠ V231), V227 (= V232), G228 (= G233), S229 (= S234), I232 (≠ V237), G251 (= G256), C284 (= C289), E386 (= E390), F388 (= F392)
P42412 Malonate-semialdehyde dehydrogenase; MSA dehydrogenase; Methylmalonate-semialdehyde dehydrogenase; MMSA dehydrogenase; MMSDH; MSDH; EC 1.2.1.27 from Bacillus subtilis (strain 168) (see 3 papers)
46% identity, 96% coverage: 7:490/505 of query aligns to 2:482/487 of P42412
- C36 (≠ A41) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-160; A-287; A-351 and A-413.
- R107 (= R112) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
- A150 (≠ T155) binding
- F152 (= F157) binding
- C160 (= C165) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-287; A-351 and A-413.
- K176 (= K181) binding
- E179 (= E184) binding
- R180 (= R185) binding
- S229 (= S234) binding
- T251 (≠ G256) binding
- R283 (= R288) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
- C287 (≠ T292) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-351 and A-413.
- C351 (≠ V356) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-413.
- E382 (= E390) binding
- C413 (≠ S421) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-351.
1t90A Crystal structure of methylmalonate semialdehyde dehydrogenase from bacillus subtilis
46% identity, 95% coverage: 9:490/505 of query aligns to 2:480/484 of 1t90A
- active site: N151 (= N158), K174 (= K181), L248 (= L255), C282 (= C289), E380 (= E390), A460 (= A470)
- binding nicotinamide-adenine-dinucleotide: I147 (≠ V154), A148 (≠ T155), P149 (= P156), F150 (= F157), N151 (= N158), W159 (= W166), K174 (= K181), E177 (= E184), R178 (= R185), H207 (≠ K214), V225 (= V232), G226 (= G233), S227 (= S234), V230 (= V237), L248 (= L255), T249 (≠ G256), C282 (= C289), E380 (= E390), F382 (= F392)
4o6rA Crystal structure of a putative aldehyde dehydrogenase from burkholderia cenocepacia
35% identity, 93% coverage: 15:485/505 of query aligns to 7:475/489 of 4o6rA
- active site: N150 (= N158), K173 (= K181), E248 (≠ L255), C282 (= C289), E383 (= E390), E460 (≠ A470)
- binding adenosine monophosphate: I146 (≠ V154), V147 (≠ T155), K173 (= K181), G206 (= G213), G210 (≠ V217), Q211 (≠ D218), F224 (≠ V231), G226 (= G233), S227 (= S234), T230 (≠ V237), R233 (≠ Y240)
6wsbA Crystal structure of a betaine aldehyde dehydrogenase from burkholderia pseudomallei bound to cofactor NAD (see paper)
35% identity, 93% coverage: 15:486/505 of query aligns to 9:478/489 of 6wsbA
- active site: N152 (= N158), E250 (≠ L255), C284 (= C289), E462 (≠ H468)
- binding nicotinamide-adenine-dinucleotide: I148 (≠ V154), G149 (≠ T155), A150 (≠ P156), W151 (≠ F157), N152 (= N158), K175 (= K181), E178 (= E184), G208 (≠ K214), G211 (≠ V217), A212 (≠ D218), F225 (≠ V231), T226 (≠ V232), G227 (= G233), G228 (≠ S234), T231 (≠ V237), V235 (≠ I241), E250 (≠ L255), L251 (≠ G256), G252 (≠ S257), C284 (= C289), E385 (= E390), F387 (= F392)
4go4A Crystal structure of pnpe in complex with nicotinamide adenine dinucleotide
35% identity, 95% coverage: 15:495/505 of query aligns to 6:480/487 of 4go4A
- active site: N149 (= N158), K172 (= K181), E247 (≠ L255), C281 (= C289), E381 (= E390), E458 (≠ H468)
- binding nicotinamide-adenine-dinucleotide: I145 (≠ V154), V146 (≠ T155), W148 (≠ F157), N149 (= N158), F154 (≠ V163), K172 (= K181), G205 (= G213), G209 (≠ V217), Q210 (≠ D218), F223 (≠ V231), T224 (≠ V232), G225 (= G233), S226 (= S234), T229 (≠ V237), E247 (≠ L255), G249 (≠ S257), C281 (= C289), E381 (= E390), F383 (= F392)
1bpwA Betaine aldehyde dehydrogenase from cod liver (see paper)
31% identity, 97% coverage: 2:491/505 of query aligns to 11:498/503 of 1bpwA
- active site: N166 (= N158), K189 (= K181), E263 (≠ L255), C297 (= C289), E400 (= E390), E477 (≠ H468)
- binding nicotinamide-adenine-dinucleotide: I162 (≠ V154), L163 (≠ T155), W165 (≠ F157), N166 (= N158), K189 (= K181), G221 (= G213), G225 (≠ V217), T240 (≠ V232), G241 (= G233), S242 (= S234), T245 (≠ V237), E263 (≠ L255), L264 (≠ G256), C297 (= C289), E400 (= E390), F402 (= F392), F466 (= F457)
P56533 4-trimethylaminobutyraldehyde dehydrogenase; TMABA-DH; TMABADH; Aldehyde dehydrogenase family 9 member A1; Betaine aldehyde dehydrogenase; BADH; EC 1.2.1.47; EC 1.2.1.3 from Gadus morhua subsp. callarias (Baltic cod) (Gadus callarias) (see paper)
31% identity, 97% coverage: 2:491/505 of query aligns to 11:498/503 of P56533
8vr1A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (ctp bound)
34% identity, 94% coverage: 11:485/505 of query aligns to 4:477/488 of 8vr1A
8vr0A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (gmp bound)
34% identity, 94% coverage: 11:485/505 of query aligns to 4:477/488 of 8vr0A
8vqzA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (cmp bound)
34% identity, 94% coverage: 11:485/505 of query aligns to 4:477/488 of 8vqzA
8vqwC Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (coa bound)
34% identity, 94% coverage: 11:485/505 of query aligns to 4:477/488 of 8vqwC
- binding coenzyme a: I147 (≠ V154), W150 (≠ F157), K174 (= K181), S176 (= S183), E177 (= E184), G207 (= G213), G211 (≠ V217), F225 (≠ V231), G227 (= G233), G228 (≠ S234), S231 (≠ V237), H331 (= H336), F387 (= F392)
8vj3A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (fad bound)
34% identity, 94% coverage: 11:485/505 of query aligns to 4:477/488 of 8vj3A
8uzoA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (adp bound)
34% identity, 94% coverage: 11:485/505 of query aligns to 4:477/488 of 8uzoA
8uznA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (amp bound)
34% identity, 94% coverage: 11:485/505 of query aligns to 4:477/488 of 8uznA
- binding adenosine monophosphate: I147 (≠ V154), G148 (≠ T155), K174 (= K181), S176 (= S183), E177 (= E184), G207 (= G213), G211 (≠ V217), F225 (≠ V231), G228 (≠ S234), S231 (≠ V237), K234 (≠ Y240)
8uzmA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (NADPH bound)
34% identity, 94% coverage: 11:485/505 of query aligns to 4:477/488 of 8uzmA
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G148 (≠ T155), W150 (≠ F157), K174 (= K181), S176 (= S183), E177 (= E184), G207 (= G213), G211 (≠ V217), F225 (≠ V231), T226 (≠ V232), G227 (= G233), G228 (≠ S234), S231 (≠ V237), E250 (≠ L255), G252 (≠ S257), C284 (= C289), E385 (= E390), F387 (= F392)
8uzkA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (NADP+ bound)
34% identity, 94% coverage: 11:485/505 of query aligns to 4:477/488 of 8uzkA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I147 (≠ V154), G148 (≠ T155), W150 (≠ F157), N151 (= N158), K174 (= K181), S176 (= S183), E177 (= E184), G207 (= G213), G211 (≠ V217), F225 (≠ V231), G227 (= G233), G228 (≠ S234), S231 (≠ V237), E250 (≠ L255), F387 (= F392)
8skfA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (lattice translocation disorder)
34% identity, 94% coverage: 11:485/505 of query aligns to 13:486/497 of 8skfA
- binding calcium ion: T33 (≠ V31), I34 (≠ F32), D100 (= D98), V187 (≠ R185)
- binding nicotinamide-adenine-dinucleotide: I156 (≠ V154), G157 (≠ T155), A158 (≠ P156), W159 (≠ F157), K183 (= K181), E186 (= E184), G216 (= G213), G220 (≠ V217), T235 (≠ V232), G236 (= G233), G237 (≠ S234), S240 (≠ V237), K243 (≠ Y240), E259 (≠ L255), C293 (= C289), F396 (= F392)
Query Sequence
>BPHYT_RS28150 BPHYT_RS28150 methylmalonate-semialdehyde dehydrogenase
MSEGNQAAVRELSHFIDGRRAAGASGRFGDVFDPAQGRVAARVPVANATEVDAAVAAAKA
AFPAWSETAPLKRARLMFRFKELLEAHSDELAELITRDHGKLLSDAKGEVVRGIEIVEFA
CGIPNLLKTDFTDQISGGIDNWNLRQPLGVAAGVTPFNFPMVVPCWMFVMAAACGNTFVL
KPSERTPSASIRLAELFIEAGFPKGVFNVVHGGKAVVDALIEHPDVAAISVVGSTPVAEY
IYSESAKRGKRVQALGSAKNHLVVMPDADLDQAVDALIASSYGSAGERCMATSVAVAVGR
IGDELIERLAPRVRSLRIGGGMEPDLDMGPLISAAHRAKVLGYIDAGVAAGARLVVDGRG
HSVPGHEAGFFLGGSLFDDVKPDMSIYREEIFGPVLSVVRVPDLASAIALVNAHELGNCV
SLFTSDGGAARAFSRQIQIGMVGINVPSPVPPAWHSFGGWKRSLFGDHHAYGEEAVRFYT
HYKSVMQRWPDSIAKGAEFAMPVAH
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory