SitesBLAST

SAGL 155:158 (≠ TSSF 161:164) mutation Missing: Low pungency phenotype, characterized by low levels of capsaicinoids, and accumulation of high levels of capsinoids, which are non-pungent capsaicinoid analogs.

6io1B Crystal structure of a novel thermostable (s)-enantioselective omega- transaminase from thermomicrobium roseum (see paper)
31% identity, 95% coverage: 23:442/442 of query aligns to 11:443/448 of 6io1B

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active site: L20 (= L32), Y151 (= Y154), D257 (= D255), K286 (= K285)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (≠ T126), G118 (= G127), A119 (≠ S128), N122 (= N131), Y151 (= Y154), H152 (= H155), D257 (= D255), V259 (= V257), I260 (≠ Q258), K286 (= K285)

Q94CE5 Gamma-aminobutyrate transaminase POP2, mitochondrial; AtGABA-T; Gamma-aminobutyric acid aminotransferase 1; Protein HEXENAL RESPONSE 1; Protein POLLEN-PISTIL INCOMPATIBILITY 2; AtPOP2; EC 2.6.1.96 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
28% identity, 90% coverage: 42:441/442 of query aligns to 69:490/504 of Q94CE5

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C98 (= C71) mutation to Y: In her1-1; loss of E2-hexenal responses.
G103 (≠ H74) mutation to N: In her1-2; loss of E2-hexenal responses.
G271 (≠ D228) mutation to E: In pop2-6; Loss of activity and complete sterility.
G312 (= G270) mutation to D: In pop2-7; loss of activity.

O50131 Ornithine aminotransferase; Orn-AT; Ornithine delta-aminotransferase; EC 2.6.1.13 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
32% identity, 87% coverage: 46:428/442 of query aligns to 40:431/454 of O50131

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A

E

N

E

D

E

A

A

T92 (= T94) mutation to V: Slightly increases the specific activity. Increases KM for L-ornithine.
D93 (≠ R95) mutation to L: Reduces the specific activity. Increases KM for L-ornithine.
G124 (= G127) binding
T125 (≠ S128) binding
Q267 (= Q258) binding
K293 (= K285) modified: N6-(pyridoxal phosphate)lysine
T321 (= T315) binding

7vo1A Structure of aminotransferase-substrate complex (see paper)
32% identity, 87% coverage: 46:428/442 of query aligns to 38:429/452 of 7vo1A

query
sites
7vo1A

I

I

V

E

R

R

G

A

E

E

K

G

V

V

Y

Y

L

W

Y

I

D

D

D

V

Q

D

G

G

N

N

D

V

Y

L

L

L

D

D

A

F

Y

S

N

S

N

G

-
x
I

-

G

V

V

V

M

C

N

V

V

G

G

H

L

A

R

N

N

P

P

R

K

I

V

V

I

D

E

A

A

V

I

T

K

R

K

Q

Q

L

L

S

D

T

-

L

L

C

V

T

L

H

H

-

A

-

G

T

T

R

D

Y

Y

M

Y

Q

N

E

P

P

Y

I

Q

L

V

D

E

Y

L

A

A

E

K

D

K

L

L

L

V

S

E

T

I

F

A

N

P

T

G

S

D

I

I

R

E

A

R

G

-

Q

K

M

V

M

F

F

L

T

S

C

N

T
x
S

G
|
G

S
x
T

E

E

A

A

N

N

D

E

L

A

A

A

T

L

R

K

I

I

A

A

M

K

Q

W

Y

S

A

T

G

N

K

R

T

K

G

M

V

F

I

I

V

A

T

F

S

I

E

G

A

A

Y
x
F

H
|
H

G

G

N
x
R

S

T

H

H

L

G

T

T

S

M

S

S

F

L

S

T

P

A

S

S

L

K

-

P

-

V

-

Q

-

R

G

S

R

R

R

M

A

F

L

P

L

T

G

M

P

P

Y

G

V

V

R

V

T

H

V

V

P

P

A

Y

P

P

D

N

S

P

Y

Y

R

R

-

N

-

P

-

W

-

G

-

I

-

D

-

G

-

Y

M

E

T

N

P

P

S

D

E

E

I

L

G

I

Q

N

R

R

M

V

A

I

A

D

Q

Y

V

I

A

E

L

E

Q

Y

I

L

E

F

D

E

I

H

R

Y

R

V

H

P

G

A

G

E

L

V

A

A

A

G

F

I

I

F

A

F

D

E

S

P

F

I

F

Q

S

G

S
x
E

D

G

G

G

V

Y

F

V

A

V

H

P

P

P

T

K

D

N

V

F

L

F

A

K

P

E

V

L

A

K

E

K

V

L

V

A

R

D

R

K

A

H

G

G

G

I

L

L

F

L

I

I

A

D

D
|
D

E

E

V
|
V

Q
|
Q

S

M

G

G

F

M

G

G

R

R

S

T

G

G

T

-

H

R

M

M

W

W

G

A

H

I

E

E

R

H

H

F

G

D

V

I

V

V

P

P

D

D

I

I

V

V

T

T

L

V

G

A

K
|
K

P

A

M

L

G

G

N

G

G

G

Y

I

P

P

V

I

A

G

G

A

L

T

V

I

V

F

R

R

P

A

E

D

V

L

V

-

A

-

G

D

F

F

G

G

Q

V

D

S

M

G

R

V

Y

H

F

S

N
|
N

T
|
T

F

F

G

G

N

N

S

T

V

V

A

A

I

A

A

A

Q

L

A

A

T

V

L

I

D

E

V

E

L

L

R

Q

D

N

E

-

H

G

V

L

L

I

D

E

N

N

A

A

Q

Q

R

K

V

L

G

E

A

P

I

L

L

F

A

R

E

E

G

R

L

L

N

E

A

E

L

M

A

K

R

E

K

K

Y

Y

E

E

C

I

I

I

G

G

D

D

V

V

R

R

G

G

T

L

G

G

L

L

Y

A

F

W

G

G

V

V

E

E

I

F

V

V

R

K

D

D

R

R

A

K

K

T

K

K

D

E

T

Y

D

A

I

T

A

K

T

E

A

R

L

G

K

E

I

I

V

V

N

V

G

E

L

A

R

L

Q

K

R

R

R

G

V

L

L

A

I

L

S

L

A

G

T

C

G

G

P

-

D

-

A

K

S

S

V

A

L

I

K
x
R

I

L

R

I

P

P

L

L

V

I

F

I

G

S

A

E

N

E

D

E

A

A

binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: I61 (vs. gap), S121 (≠ T126), G122 (= G127), T123 (≠ S128), F149 (≠ Y154), H150 (= H155), R152 (≠ N157), E234 (≠ S227), D262 (= D255), V264 (= V257), Q265 (= Q258), K291 (= K285), N318 (= N314), T319 (= T315), R417 (≠ K416)

7vntA Structure of aminotransferase-substrate complex (see paper)
32% identity, 87% coverage: 46:428/442 of query aligns to 38:429/452 of 7vntA

query
sites
7vntA

I

I

V

E

R

R

G

A

E

E

K

G

V

V

Y

Y

L

W

Y

I

D

D

D

V

Q

D

G

G

N

N

D

V

Y

L

L

L

D

D

A

F

Y

S

N

S

N

G

-

I

-

G

V

V

V

M

C

N

V

V

G

G

H

L

A

R

N

N

P

P

R

K

I

V

V

I

D

E

A

A

V

I

T

K

R

K

Q

Q

L

L

S

D

T

-

L

L

C

V

T

L

H

H

-

A

-

G

T

T

R

D

Y

Y

M

Y

Q

N

E

P

P

Y

I

Q

L

V

D

E

Y

L

A

A

E

K

D

K

L

L

L

V

S

E

T

I

F

A

N

P

T

G

S

D

I

I

R

E

A

R

G

-

Q

K

M

V

M

F

F

L

T

S

C

N

T

S

G
|
G

S
x
T

E

E

A

A

N

N

D

E

L

A

A

A

T

L

R

K

I

I

A

A

M

K

Q

W

Y

S

A

T

G

N

K

R

T

K

G

M

V

F

I

I

V

A

T

F

S

I

E

G

A

A

Y
x
F

H
|
H

G

G

N
x
R

S

T

H

H

L

G

T

T

S

M

S

S

F

L

S

T

P

A

S

S

L

K

-

P

-

V

-

Q

-

R

G

S

R

R

R

M

A

F

L

P

L

T

G

M

P

P

Y

G

V

V

R

V

T

H

V

V

P

P

A

Y

P

P

D

N

S

P

Y

Y

R

R

-

N

-

P

-

W

-

G

-

I

-

D

-

G

-

Y

M

E

T

N

P

P

S

D

E

E

I

L

G

I

Q

N

R

R

M

V

A

I

A

D

Q

Y

V

I

A

E

L

E

Q

Y

I

L

E

F

D

E

I

H

R

Y

R

V

H

P

G

A

G

E

L

V

A

A

A

G

F

I

I

F

A

F

D
x
E

S

P

F

I

F

Q

S

G

S
x
E

D

G

G

G

V

Y

F

V

A

V

H

P

P

P

T

K

D

N

V

F

L

F

A

K

P

E

V

L

A

K

E

K

V

L

V

A

R

D

R

K

A

H

G

G

G

I

L

L

F

L

I

I

A

D

D
|
D

E

E

V
|
V

Q
|
Q

S

M

G

G

F

M

G

G

R

R

S

T

G

G

T

-

H

R

M

M

W

W

G

A

H

I

E

E

R

H

H

F

G

D

V

I

V

V

P

P

D

D

I

I

V

V

T

T

L

V

G

A

K
|
K

P

A

M

L

G

G

N

G

G

G

Y

I

P

P

V

I

A

G

G

A

L

T

V

I

V

F

R

R

P

A

E

D

V

L

V

-

A

-

G

D

F

F

G

G

Q

V

D

S

M

G

R

V

Y

H

F

S

N

N

T

T

F

F

G

G

N

N

S

T

V

V

A

A

I

A

A

A

Q

L

A

A

T

V

L

I

D

E

V

E

L

L

R

Q

D

N

E

-

H

G

V

L

L

I

D

E

N

N

A

A

Q

Q

R

K

V

L

G

E

A

P

I

L

L

F

A

R

E

E

G

R

L

L

N

E

A

E

L

M

A

K

R

E

K

K

Y

Y

E

E

C

I

I

I

G

G

D

D

V

V

R

R

G

G

T

L

G

G

L

L

Y

A

F

W

G

G

V

V

E

E

I

F

V

V

R

K

D

D

R

R

A

K

K

T

K

K

D

E

T

Y

D

A

I

T

A

K

T

E

A

R

L

G

K

E

I

I

V

V

N

V

G

E

L

A

R

L

Q

K

R

R

R

G

V

L

L

A

I

L

S

L

A

G

T

C

G

G

P

-

D

-

A

K

S

S

V

A

L

I

K

R

I

L

R

I

P

P

L

L

V

I

F

I

G

S

A

E

N

E

D

E

A

A

binding L-ornithine: F149 (≠ Y154), R152 (≠ N157), E234 (≠ S227), K291 (= K285)
binding pyridoxal-5'-phosphate: G122 (= G127), T123 (≠ S128), F149 (≠ Y154), H150 (= H155), E229 (≠ D222), D262 (= D255), V264 (= V257), Q265 (= Q258), K291 (= K285)

7vnoA Structure of aminotransferase (see paper)
32% identity, 87% coverage: 46:428/442 of query aligns to 38:429/452 of 7vnoA

query
sites
7vnoA

I

I

V

E

R

R

G

A

E

E

K

G

V

V

Y

Y

L

W

Y

I

D

D

D

V

Q

D

G

G

N

N

D

V

Y

L

L

L

D

D

A

F

Y

S

N

S

N

G

-

I

-

G

V

V

V

M

C

N

V

V

G

G

H

L

A

R

N

N

P

P

R

K

I

V

V

I

D

E

A

A

V

I

T

K

R

K

Q

Q

L

L

S

D

T

-

L

L

C

V

T

L

H

H

-

A

-

G

T

T

R

D

Y

Y

M

Y

Q

N

E

P

P

Y

I

Q

L

V

D

E

Y

L

A

A

E

K

D

K

L

L

L

V

S

E

T

I

F

A

N

P

T

G

S

D

I

I

R

E

A

R

G

-

Q

K

M

V

M

F

F

L

T

S

C

N

T

S

G
|
G

S
x
T

E

E

A

A

N
|
N

D

E

L

A

A

A

T

L

R

K

I

I

A

A

M

K

Q

W

Y

S

A

T

G

N

K

R

T

K

G

M

V

F

I

I

V

A

T

F

S

I

E

G

A

A

Y
x
F

H
|
H

G

G

N

R

S

T

H

H

L

G

T

T

S

M

S

S

F

L

S

T

P

A

S

S

L

K

-

P

-

V

-

Q

-

R

G

S

R

R

R

M

A

F

L

P

L

T

G

M

P

P

Y

G

V

V

R

V

T

H

V

V

P

P

A

Y

P

P

D

N

S

P

Y

Y

R

R

-

N

-

P

-

W

-

G

-

I

-

D

-

G

-

Y

M

E

T

N

P

P

S

D

E

E

I

L

G

I

Q

N

R

R

M

V

A

I

A

D

Q

Y

V

I

A

E

L

E

Q

Y

I

L

E

F

D

E

I

H

R

Y

R

V

H

P

G

A

G

E

L

V

A

A

A

G

F

I

I

F

A

F

D
x
E

S

P

F

I

F

Q

S

G

S

E

D

G

G

G

V

Y

F

V

A

V

H

P

P

P

T

K

D

N

V

F

L

F

A

K

P

E

V

L

A

K

E

K

V

L

V

A

R

D

R

K

A

H

G

G

G

I

L

L

F

L

I

I

A

D

D
|
D

E

E

V
|
V

Q
|
Q

S

M

G

G

F

M

G

G

R

R

S

T

G

G

T

-

H

R

M

M

W

W

G

A

H

I

E

E

R

H

H

F

G

D

V

I

V

V

P

P

D

D

I

I

V

V

T

T

L

V

G

A

K
|
K

P

A

M

L

G

G

N

G

G

G

Y

I

P

P

V

I

A

G

G

A

L

T

V

I

V

F

R

R

P

A

E

D

V

L

V

-

A

-

G

D

F

F

G

G

Q

V

D

S

M

G

R

V

Y

H

F

S

N

N

T

T

F

F

G

G

N

N

S

T

V

V

A

A

I

A

A

A

Q

L

A

A

T

V

L

I

D

E

V

E

L

L

R

Q

D

N

E

-

H

G

V

L

L

I

D

E

N

N

A

A

Q

Q

R

K

V

L

G

E

A

P

I

L

L

F

A

R

E

E

G

R

L

L

N

E

A

E

L

M

A

K

R

E

K

K

Y

Y

E

E

C

I

I

I

G

G

D

D

V

V

R

R

G

G

T

L

G

G

L

L

Y

A

F

W

G

G

V

V

E

E

I

F

V

V

R

K

D

D

R

R

A

K

K

T

K

K

D

E

T

Y

D

A

I

T

A

K

T

E

A

R

L

G

K

E

I

I

V

V

N

V

G

E

L

A

R

L

Q

K

R

R

R

G

V

L

L

A

I

L

S

L

A

G

T

C

G

G

P

-

D

-

A

K

S

S

V

A

L

I

K

R

I

L

R

I

P

P

L

L

V

I

F

I

G

S

A

E

N

E

D

E

A

A

binding pyridoxal-5'-phosphate: G122 (= G127), T123 (≠ S128), N126 (= N131), F149 (≠ Y154), H150 (= H155), E229 (≠ D222), D262 (= D255), V264 (= V257), Q265 (= Q258), K291 (= K285)

1zobA Crystal structure of dialkylglycine decarboxylases bound with calcium ion
31% identity, 89% coverage: 42:435/442 of query aligns to 23:423/431 of 1zobA

query
sites
1zobA

E

E

P

P

V

M

K

I

I

I

V

E

R

R

G

A

E

K

K

G

V

S

Y

F

L

V

Y

Y

D

D

D

A

Q

D

G

G

N

R

D

A

Y

I

L

L

D

D

A

F

Y

T

N

S

N

G

V

Q

V

M

-

S

-

A

C

V

V

L

G

G

H

H

A

C

N

H

P

P

R

E

I

I

V

V

D

S

A

V

V

I

T

G

R

E

Q

Y

L

A

S

G

T

K

L

L

-

D

C

H

T
x
L

H

F

T
x
S

R

E

Y

M

M

L

Q

S

E

R

P

P

I

V

L

V

D

D

Y

L

A

A

E

T

D

R

L

L

L

A

S

N

T

I

F

T

N

P

T

P

S

G

I

L

R

D

A

-

G

-

Q

R

M

A

M

L

F

L

T

L

C

S

T
|
T

G

G

S
x
A

E

E

A

S

N
|
N

D

E

L

A

A

A

T

I

R

R

I

M

A

A

M

K

Q

L

Y

V

A

T

G

G

K

K

T

Y

G

E

V

I

I

V

V

G

T

F

S

A

E

Q

A

S

Y
x
W

H
|
H

G

G

N

M

S

T

H

G

L

A

T

A

S

A

S

S

F

A

S

T

P

Y

S

S

L

A

G

G

R

R

R

K

A

G

L

V

L

-

G

G

P

P

Y

A

V

A

R

V

-

G

-

S

-

F

T

A

V

I

P

P

A

A

P

P

D

F

S

T

Y

Y

R

R

M

P

T

R

P

F

S

E

E

R

I

N

G

G

Q

A

R

Y

-

D

M

Y

A

L

A

A

Q

E

V

L

A

D

L

Y

Q

A

I

F

E

D

D

L

I

I

-

D

R

R

R

Q

H

S

G

S

G

G

G

N

L

L

A

A

F

F

I

I

A

A

D
x
E

S

P

F

I

F

L

S

S

D

G

G

G

V

I

F

I

A

E

H

L

P

P

T

D

D

G

V

Y

L

M

A

A

P

A

V

L

A

K

E

R

V

K

V

C

R

E

R

A

A

R

G

G

G

M

L

L

F

L

I

I

A

L

D
|
D

E

E

V
x
A

Q
|
Q

S

T

G

G

F

V

G

G

R

R

S

T

G

G

T

T

H

-

M

M

W

F

G

A

H

C

E

Q

R

R

H

D

G

G

V

V

V

T

P

P

D

D

I

I

V

L

T

T

L

L

G

S

K
|
K

P

T

M

L

G

G

N

A

G

G

Y

L

P

P

V

L

A

A

G

A

L

-

V

I

V

V

R

T

P

S

E

A

V

A

V

I

A

E

G

E

F

R

G

A

Q

H

D

E

M

L

R

G

Y

Y

-

L

-

F

F

Y

N

T

T
|
T

F

H

G
x
V

G
x
S

N
x
D

S

P

V

L

A

P

I

A

A

A

A

V

A

G

Q

L

A

R

T

V

L

L

D

D

V

V

L

V

R

Q

D

R

E

D

H

G

V

L

L

V

D

A

N

R

A

A

Q

N

R

V

V

M

G

G

A

D

I

R

L

L

A

R

E

R

G

G

L

L

N

L

A

D

L

L

A

M

R

E

K

R

Y

F

E

D

C

C

I

I

G

G

D

D

V

V

R

R

G

G

T

R

G

G

L

L

Y

L

F

L

G

G

V

V

E

E

I

I

V

V

R

K

D

D

R

R

A

R

K

T

K

K

D

E

T

P

D

A

I

D

A

G

T

L

A

G

L

A

K

K

I

I

V

T

N

R

-

E

-

C

-

M

-

N

-

L

G

G

L

L

R

S

Q

M

R

N

R

I

V

V

L

Q

I

L

S

P

A

G

T

M

G

G

P

-

D

-

A

-

S

G

V

V

L

F

K
x
R

I

I

R

A

P

P

L

L

V

T

F

V

G

S

A

E

N

D

D

E

A

I

D

D

R

L

L

G

L

L

T

S

E

L

L

L

active site: W136 (≠ Y154), E208 (≠ D222), D241 (= D255), Q244 (= Q258), K270 (= K285), T301 (= T315), R404 (≠ K416)
binding calcium ion: L76 (≠ T92), S78 (≠ T94), V303 (≠ G317), S304 (≠ G318), D305 (≠ N319)
binding pyridoxal-5'-phosphate: T108 (= T126), A110 (≠ S128), N113 (= N131), W136 (≠ Y154), H137 (= H155), E208 (≠ D222), D241 (= D255), A243 (≠ V257), Q244 (= Q258), K270 (= K285)

Sites not aligning to the query:

active site: 19

1zc9A The crystal structure of dialkylglycine decarboxylase complex with pyridoxamine 5-phosphate (see paper)
31% identity, 89% coverage: 42:435/442 of query aligns to 23:423/431 of 1zc9A

query
sites
1zc9A

E

E

P

P

V

M

K

I

I

I

V

E

R

R

G

A

E

K

K

G

V

S

Y

F

L

V

Y

Y

D

D

D

A

Q

D

G

G

N

R

D

A

Y

I

L

L

D

D

A

F

Y

T

N

S

N

G

V

Q

V

M

-

S

-

A

C

V

V

L

G

G

H

H

A

C

N

H

P

P

R

E

I

I

V

V

D

S

A

V

V

I

T

G

R

E

Q

Y

L

A

S

G

T

K

L

L

-

D

C

H

T

L

H

F

T

S

R

E

Y

M

M

L

Q

S

E

R

P

P

I

V

L

V

D

D

Y

L

A

A

E

T

D

R

L

L

L

A

S

N

T

I

F

T

N

P

T

P

S

G

I

L

R

D

A

-

G

-

Q

R

M

A

M

L

F

L

T

L

C

S

T

T

G
|
G

S
x
A

E

E

A

S

N

N

D

E

L

A

A

A

T

I

R

R

I

M

A

A

M

K

Q

L

Y

V

A

T

G

G

K

K

T

Y

G

E

V

I

I

V

V

G

T

F

S

A

E

Q

A

S

Y
x
W

H
|
H

G

G

N

M

S

T

H

G

L

A

T

A

S

A

S

S

F

A

S

T

P

Y

S

S

L

A

G

G

R

R

R

K

A

G

L

V

L

-

G

G

P

P

Y

A

V

A

R

V

-

G

-

S

-

F

T

A

V

I

P

P

A

A

P

P

D

F

S

T

Y

Y

R

R

M

P

T

R

P

F

S

E

E

R

I

N

G

G

Q

A

R

Y

-

D

M

Y

A

L

A

A

Q

E

V

L

A

D

L

Y

Q

A

I

F

E

D

D

L

I

I

-

D

R

R

R

Q

H

S

G

S

G

G

G

N

L

L

A

A

F

F

I

I

A

A

D
x
E

S

P

F

I

F

L

S

S

D

G

G

G

V

I

F

I

A

E

H

L

P

P

T

D

D

G

V

Y

L

M

A

A

P

A

V

L

A

K

E

R

V

K

V

C

R

E

R

A

A

R

G

G

G

M

L

L

F

L

I

I

A

L

D
|
D

E

E

V
x
A

Q
|
Q

S

T

G

G

F

V

G

G

R

R

S

T

G

G

T

T

H

-

M

M

W

F

G

A

H

C

E

Q

R

R

H

D

G

G

V

V

V

T

P

P

D

D

I

I

V

L

T

T

L

L

G

S

K
|
K

P

T

M

L

G

G

N

A

G

G

Y

L

P

P

V

L

A

A

G

A

L

-

V

I

V

V

R

T

P

S

E

A

V

A

V

I

A

E

G

E

F

R

G

A

Q

H

D

E

M

L

R

G

Y

Y

-

L

-

F

F

Y

N

T

T
|
T

F

H

G

V

G

S

N

D

S

P

V

L

A

P

I

A

A

A

A

V

A

G

Q

L

A

R

T

V

L

L

D

D

V

V

L

V

R

Q

D

R

E

D

H

G

V

L

L

V

D

A

N

R

A

A

Q

N

R

V

V

M

G

G

A

D

I

R

L

L

A

R

E

R

G

G

L

L

N

L

A

D

L

L

A

M

R

E

K

R

Y

F

E

D

C

C

I

I

G

G

D

D

V

V

R

R

G

G

T

R

G

G

L

L

Y

L

F

L

G

G

V

V

E

E

I

I

V

V

R

K

D

D

R

R

A

R

K

T

K

K

D

E

T

P

D

A

I

D

A

G

T

L

A

G

L

A

K

K

I

I

V

T

N

R

-

E

-

C

-

M

-

N

-

L

G

G

L

L

R

S

Q

M

R

N

R

I

V

V

L

Q

I

L

S

P

A

G

T

M

G

G

P

-

D

-

A

-

S

G

V

V

L

F

K
x
R

I

I

R

A

P

P

L

L

V

T

F

V

G

S

A

E

N

D

D

E

A

I

D

D

R

L

L

G

L

L

T

S

E

L

L

L

active site: W136 (≠ Y154), E208 (≠ D222), D241 (= D255), Q244 (= Q258), K270 (= K285), T301 (= T315), R404 (≠ K416)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G109 (= G127), A110 (≠ S128), W136 (≠ Y154), H137 (= H155), E208 (≠ D222), D241 (= D255), A243 (≠ V257), Q244 (= Q258), K270 (= K285)

Sites not aligning to the query:

active site: 19

1m0qA Structure of dialkylglycine decarboxylase complexed with s-1- aminoethanephosphonate (see paper)
31% identity, 89% coverage: 42:435/442 of query aligns to 23:423/431 of 1m0qA

query
sites
1m0qA

E

E

P

P

V

M

K

I

I

I

V

E

R

R

G

A

E

K

K

G

V

S

Y

F

L

V

Y

Y

D

D

D

A

Q

D

G

G

N

R

D

A

Y

I

L

L

D

D

A

F

Y

T

N

S

N

G

V
x
Q

V

M

-

S

-

A

C

V

V

L

G

G

H

H

A

C

N

H

P

P

R

E

I

I

V

V

D

S

A

V

V

I

T

G

R

E

Q

Y

L

A

S

G

T

K

L

L

-

D

C

H

T

L

H

F

T

S

R

E

Y

M

M

L

Q

S

E

R

P

P

I

V

L

V

D

D

Y

L

A

A

E

T

D

R

L

L

L

A

S

N

T

I

F

T

N

P

T

P

S

G

I

L

R

D

A

-

G

-

Q

R

M

A

M

L

F

L

T

L

C

S

T
|
T

G

G

S
x
A

E

E

A

S

N

N

D

E

L

A

A

A

T

I

R

R

I

M

A

A

M

K

Q

L

Y

V

A

T

G

G

K

K

T

Y

G

E

V

I

I

V

V

G

T

F

S

A

E

Q

A

S

Y
x
W

H
|
H

G

G

N

M

S

T

H

G

L

A

T

A

S

A

S

S

F

A

S

T

P

Y

S

S

L

A

G

G

R

R

R

K

A

G

L

V

L

-

G

G

P

P

Y

A

V

A

R

V

-

G

-

S

-

F

T

A

V

I

P

P

A

A

P

P

D

F

S

T

Y

Y

R

R

M

P

T

R

P

F

S

E

E

R

I

N

G

G

Q

A

R

Y

-

D

M

Y

A

L

A

A

Q

E

V

L

A

D

L

Y

Q

A

I

F

E

D

D

L

I

I

-

D

R

R

R

Q

H

S

G

S

G

G

G

N

L

L

A

A

F

F

I

I

A

A

D
x
E

S

P

F

I

F

L

S

S

S
|
S

D

G

G

G

V

I

F

I

A

E

H

L

P

P

T

D

D

G

V

Y

L

M

A

A

P

A

V

L

A

K

E

R

V

K

V

C

R

E

R

A

A

R

G

G

G

M

L

L

F

L

I

I

A

L

D
|
D

E

E

V
x
A

Q
|
Q

S

T

G

G

F

V

G

G

R

R

S

T

G

G

T

T

H

-

M

M

W

F

G

A

H

C

E

Q

R

R

H

D

G

G

V

V

V

T

P

P

D

D

I

I

V

L

T

T

L

L

G

S

K
|
K

P

T

M

L

G

G

N

A

G

G

Y

L

P

P

V

L

A

A

G

A

L

-

V

I

V

V

R

T

P

S

E

A

V

A

V

I

A

E

G

E

F

R

G

A

Q

H

D

E

M

L

R

G

Y

Y

-

L

-

F

F

Y

N

T

T
|
T

F

H

G

V

G

S

N

D

S

P

V

L

A

P

I

A

A

A

A

V

A

G

Q

L

A

R

T

V

L

L

D

D

V

V

L

V

R

Q

D

R

E

D

H

G

V

L

L

V

D

A

N

R

A

A

Q

N

R

V

V

M

G

G

A

D

I

R

L

L

A

R

E

R

G

G

L

L

N

L

A

D

L

L

A

M

R

E

K

R

Y

F

E

D

C

C

I

I

G

G

D

D

V

V

R

R

G

G

T

R

G

G

L

L

Y

L

F

L

G

G

V

V

E

E

I

I

V

V

R

K

D

D

R

R

A

R

K

T

K

K

D

E

T

P

D

A

I

D

A

G

T

L

A

G

L

A

K

K

I

I

V

T

N

R

-

E

-

C

-

M

-

N

-

L

G

G

L

L

R

S

Q

M

R

N

R

I

V

V

L

Q

I

L

S

P

A

G

T

M

G

G

P

-

D

-

A

-

S

G

V

V

L

F

K
x
R

I

I

R

A

P

P

L

L

V

T

F

V

G

S

A

E

N

D

D

E

A

I

D

D

R

L

L

G

L

L

T

S

E

L

L

L

active site: W136 (≠ Y154), E208 (≠ D222), D241 (= D255), Q244 (= Q258), K270 (= K285), T301 (= T315), R404 (≠ K416)
binding (1s)-1-[((1e)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylene)amino]ethylphosphonic acid: Q50 (≠ V69), T108 (= T126), A110 (≠ S128), W136 (≠ Y154), H137 (= H155), E208 (≠ D222), S213 (= S227), D241 (= D255), A243 (≠ V257), Q244 (= Q258), K270 (= K285), R404 (≠ K416)

Sites not aligning to the query:

active site: 19

1m0pA Structure of dialkylglycine decarboxylase complexed with 1-amino-1- phenylethanephosphonate (see paper)
31% identity, 89% coverage: 42:435/442 of query aligns to 23:423/431 of 1m0pA

query
sites
1m0pA

E

E

P

P

V

M

K

I

I

I

V

E

R

R

G

A

E

K

K

G

V

S

Y

F

L

V

Y

Y

D

D

D

A

Q

D

G

G

N

R

D

A

Y

I

L

L

D

D

A

F

Y

T

N

S

N

G

V
x
Q

V

M

-

S

-

A

C

V

V

L

G

G

H

H

A

C

N

H

P

P

R

E

I

I

V

V

D

S

A

V

V

I

T

G

R

E

Q

Y

L

A

S

G

T

K

L

L

-

D

C

H

T

L

H

F

T

S

R

E

Y

M

M

L

Q

S

E

R

P

P

I

V

L

V

D

D

Y

L

A

A

E

T

D

R

L

L

L

A

S

N

T

I

F

T

N

P

T

P

S

G

I

L

R

D

A

-

G

-

Q

R

M

A

M

L

F

L

T

L

C

S

T
|
T

G

G

S
x
A

E

E

A

S

N

N

D

E

L

A

A

A

T

I

R

R

I

M

A

A

M

K

Q

L

Y

V

A

T

G

G

K

K

T

Y

G

E

V

I

I

V

V

G

T

F

S

A

E

Q

A

S

Y
x
W

H
|
H

G

G

N

M

S

T

H

G

L

A

T

A

S

A

S

S

F

A

S

T

P

Y

S

S

L

A

G

G

R

R

R

K

A

G

L

V

L

-

G

G

P

P

Y

A

V

A

R

V

-

G

-

S

-

F

T

A

V

I

P

P

A

A

P

P

D

F

S

T

Y

Y

R

R

M

P

T

R

P

F

S

E

E

R

I

N

G

G

Q

A

R

Y

-

D

M

Y

A

L

A

A

Q

E

V

L

A

D

L

Y

Q

A

I

F

E

D

D

L

I

I

-

D

R

R

R

Q

H

S

G

S

G

G

G

N

L

L

A

A

F

F

I

I

A

A

D
x
E

S

P

F

I

F

L

S

S

S
|
S

D

G

G

G

V

I

F

I

A

E

H

L

P

P

T

D

D

G

V

Y

L

M

A

A

P

A

V

L

A

K

E

R

V

K

V

C

R

E

R

A

A

R

G

G

G

M

L

L

F

L

I

I

A

L

D
|
D

E

E

V
x
A

Q
|
Q

S

T

G

G

F

V

G

G

R

R

S

T

G

G

T

T

H

-

M

M

W

F

G

A

H

C

E

Q

R

R

H

D

G

G

V

V

V

T

P

P

D

D

I

I

V

L

T

T

L

L

G

S

K
|
K

P

T

M

L

G

G

N

A

G

G

Y

L

P

P

V

L

A

A

G

A

L

-

V

I

V

V

R

T

P

S

E

A

V

A

V

I

A

E

G

E

F

R

G

A

Q

H

D

E

M

L

R

G

Y

Y

-

L

-

F

F

Y

N

T

T
|
T

F

H

G

V

G

S

N

D

S

P

V

L

A

P

I

A

A

A

A

V

A

G

Q

L

A

R

T

V

L

L

D

D

V

V

L

V

R

Q

D

R

E

D

H

G

V

L

L

V

D

A

N

R

A

A

Q

N

R

V

V

M

G

G

A

D

I

R

L

L

A

R

E

R

G

G

L

L

N

L

A

D

L

L

A

M

R

E

K

R

Y

F

E

D

C

C

I

I

G

G

D

D

V

V

R

R

G

G

T

R

G

G

L

L

Y

L

F

L

G

G

V

V

E

E

I

I

V

V

R

K

D

D

R

R

A

R

K

T

K

K

D

E

T

P

D

A

I

D

A

G

T

L

A

G

L

A

K

K

I

I

V

T

N

R

-

E

-

C

-

M

-

N

-

L

G

G

L

L

R

S

Q

M

R

N

R

I

V

V

L

Q

I

L

S

P

A

G

T

M

G

G

P

-

D

-

A

-

S

G

V

V

L

F

K
x
R

I

I

R

A

P

P

L

L

V

T

F

V

G

S

A

E

N

D

D

E

A

I

D

D

R

L

L

G

L

L

T

S

E

L

L

L

active site: W136 (≠ Y154), E208 (≠ D222), D241 (= D255), Q244 (= Q258), K270 (= K285), T301 (= T315), R404 (≠ K416)
binding (1r)-1-[((1e)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylene)amino]-1-phenylethylphosphonic acid: Q50 (≠ V69), T108 (= T126), A110 (≠ S128), W136 (≠ Y154), H137 (= H155), E208 (≠ D222), S213 (= S227), D241 (= D255), A243 (≠ V257), Q244 (= Q258), K270 (= K285), R404 (≠ K416)

Sites not aligning to the query:

active site: 19

1m0oA Structure of dialkylglycine decarboxylase complexed with 1-amino-1- methylpropanephosphonate (see paper)
31% identity, 89% coverage: 42:435/442 of query aligns to 23:423/431 of 1m0oA

query
sites
1m0oA

E

E

P

P

V

M

K

I

I

I

V

E

R

R

G

A

E

K

K

G

V

S

Y

F

L

V

Y

Y

D

D

D

A

Q

D

G

G

N

R

D

A

Y

I

L

L

D

D

A

F

Y

T

N

S

N

G

V
x
Q

V

M

-

S

-

A

C

V

V

L

G

G

H

H

A

C

N

H

P

P

R

E

I

I

V

V

D

S

A

V

V

I

T

G

R

E

Q

Y

L

A

S

G

T

K

L

L

-

D

C

H

T

L

H

F

T

S

R

E

Y

M

M

L

Q

S

E

R

P

P

I

V

L

V

D

D

Y

L

A

A

E

T

D

R

L

L

L

A

S

N

T

I

F

T

N

P

T

P

S

G

I

L

R

D

A

-

G

-

Q

R

M

A

M

L

F

L

T

L

C

S

T

T

G
|
G

S
x
A

E

E

A

S

N

N

D

E

L

A

A

A

T

I

R

R

I

M

A

A

M

K

Q

L

Y

V

A

T

G

G

K

K

T

Y

G

E

V

I

I

V

V

G

T

F

S

A

E

Q

A

S

Y
x
W

H
|
H

G

G

N

M

S

T

H

G

L

A

T

A

S

A

S

S

F

A

S

T

P

Y

S

S

L

A

G

G

R

R

R

K

A

G

L

V

L

-

G

G

P

P

Y

A

V

A

R

V

-

G

-

S

-

F

T

A

V

I

P

P

A

A

P

P

D

F

S

T

Y

Y

R

R

M

P

T

R

P

F

S

E

E

R

I

N

G

G

Q

A

R

Y

-

D

M

Y

A

L

A

A

Q

E

V

L

A

D

L

Y

Q

A

I

F

E

D

D

L

I

I

-

D

R

R

R

Q

H

S

G

S

G

G

G

N

L

L

A

A

F

F

I

I

A

A

D
x
E

S

P

F

I

F

L

S

S

D

G

G

G

V

I

F

I

A

E

H

L

P

P

T

D

D

G

V

Y

L

M

A

A

P

A

V

L

A

K

E

R

V

K

V

C

R

E

R

A

A

R

G

G

G

M

L

L

F

L

I

I

A

L

D
|
D

E

E

V

A

Q
|
Q

S

T

G

G

F

V

G

G

R

R

S

T

G

G

T

T

H

-

M

M

W

F

G

A

H

C

E

Q

R

R

H

D

G

G

V

V

V

T

P

P

D

D

I

I

V

L

T

T

L

L

G

S

K
|
K

P

T

M

L

G

G

N

A

G

G

Y

L

P

P

V

L

A

A

G

A

L

-

V

I

V

V

R

T

P

S

E

A

V

A

V

I

A

E

G

E

F

R

G

A

Q

H

D

E

M

L

R

G

Y

Y

-

L

-

F

F

Y

N

T

T
|
T

F

H

G

V

G

S

N

D

S

P

V

L

A

P

I

A

A

A

A

V

A

G

Q

L

A

R

T

V

L

L

D

D

V

V

L

V

R

Q

D

R

E

D

H

G

V

L

L

V

D

A

N

R

A

A

Q

N

R

V

V

M

G

G

A

D

I

R

L

L

A

R

E

R

G

G

L

L

N

L

A

D

L

L

A

M

R

E

K

R

Y

F

E

D

C

C

I

I

G

G

D

D

V

V

R

R

G

G

T

R

G

G

L

L

Y

L

F

L

G

G

V

V

E

E

I

I

V

V

R

K

D

D

R

R

A

R

K

T

K

K

D

E

T

P

D

A

I

D

A

G

T

L

A

G

L

A

K

K

I

I

V

T

N

R

-

E

-

C

-

M

-

N

-

L

G

G

L

L

R

S

Q

M

R

N

R

I

V

V

L

Q

I

L

S

P

A

G

T

M

G

G

P

-

D

-

A

-

S

G

V

V

L

F

K
x
R

I

I

R

A

P

P

L

L

V

T

F

V

G

S

A

E

N

D

D

E

A

I

D

D

R

L

L

G

L

L

T

S

E

L

L

L

active site: W136 (≠ Y154), E208 (≠ D222), D241 (= D255), Q244 (= Q258), K270 (= K285), T301 (= T315), R404 (≠ K416)
binding (1r)-1-[((1e)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylene)amino]-1-methylpropylphosphonic acid: Q50 (≠ V69), G109 (= G127), A110 (≠ S128), W136 (≠ Y154), H137 (= H155), E208 (≠ D222), D241 (= D255), Q244 (= Q258), K270 (= K285), R404 (≠ K416)

Sites not aligning to the query:

active site: 19

1m0nA Structure of dialkylglycine decarboxylase complexed with 1- aminocyclopentanephosphonate (see paper)
31% identity, 89% coverage: 42:435/442 of query aligns to 23:423/431 of 1m0nA

query
sites
1m0nA

E

E

P

P

V

M

K

I

I

I

V

E

R

R

G

A

E

K

K

G

V

S

Y

F

L

V

Y

Y

D

D

D

A

Q

D

G

G

N

R

D

A

Y

I

L

L

D

D

A

F

Y

T

N

S

N

G

V
x
Q

V
x
M

-

S

-

A

C

V

V

L

G

G

H

H

A

C

N

H

P

P

R

E

I

I

V

V

D

S

A

V

V

I

T

G

R

E

Q

Y

L

A

S

G

T

K

L

L

-

D

C

H

T

L

H

F

T

S

R

E

Y

M

M

L

Q

S

E

R

P

P

I

V

L

V

D

D

Y

L

A

A

E

T

D

R

L

L

L

A

S

N

T

I

F

T

N

P

T

P

S

G

I

L

R

D

A

-

G

-

Q

R

M

A

M

L

F

L

T

L

C

S

T

T

G

G

S
x
A

E

E

A

S

N
|
N

D

E

L

A

A

A

T

I

R

R

I

M

A

A

M

K

Q

L

Y

V

A

T

G

G

K

K

T

Y

G

E

V

I

I

V

V

G

T

F

S

A

E

Q

A

S

Y
x
W

H
|
H

G

G

N

M

S

T

H

G

L

A

T

A

S

A

S

S

F

A

S

T

P

Y

S

S

L

A

G

G

R

R

R

K

A

G

L

V

L

-

G

G

P

P

Y

A

V

A

R

V

-

G

-

S

-

F

T

A

V

I

P

P

A

A

P

P

D

F

S

T

Y

Y

R

R

M

P

T

R

P

F

S

E

E

R

I

N

G

G

Q

A

R

Y

-

D

M

Y

A

L

A

A

Q

E

V

L

A

D

L

Y

Q

A

I

F

E

D

D

L

I

I

-

D

R

R

R

Q

H

S

G

S

G

G

G

N

L

L

A

A

F

F

I

I

A

A

D
x
E

S

P

F

I

F

L

S

S

S
|
S

D

G

G

G

V

I

F

I

A

E

H

L

P

P

T

D

D

G

V

Y

L

M

A

A

P

A

V

L

A

K

E

R

V

K

V

C

R

E

R

A

A

R

G

G

G

M

L

L

F

L

I

I

A

L

D
|
D

E

E

V
x
A

Q
|
Q

S

T

G

G

F

V

G

G

R

R

S

T

G

G

T

T

H

-

M

M

W

F

G

A

H

C

E

Q

R

R

H

D

G

G

V

V

V

T

P

P

D

D

I

I

V

L

T

T

L

L

G

S

K
|
K

P

T

M

L

G

G

N

A

G

G

Y

L

P

P

V

L

A

A

G

A

L

-

V

I

V

V

R

T

P

S

E

A

V

A

V

I

A

E

G

E

F

R

G

A

Q

H

D

E

M

L

R

G

Y

Y

-

L

-

F

F

Y

N

T

T
|
T

F

H

G

V

G

S

N

D

S

P

V

L

A

P

I

A

A

A

A

V

A

G

Q

L

A

R

T

V

L

L

D

D

V

V

L

V

R

Q

D

R

E

D

H

G

V

L

L

V

D

A

N

R

A

A

Q

N

R

V

V

M

G

G

A

D

I

R

L

L

A

R

E

R

G

G

L

L

N

L

A

D

L

L

A

M

R

E

K

R

Y

F

E

D

C

C

I

I

G

G

D

D

V

V

R

R

G

G

T

R

G

G

L

L

Y

L

F

L

G

G

V

V

E

E

I

I

V

V

R

K

D

D

R

R

A

R

K

T

K

K

D

E

T

P

D

A

I

D

A

G

T

L

A

G

L

A

K

K

I

I

V

T

N

R

-

E

-

C

-

M

-

N

-

L

G

G

L

L

R

S

Q

M

R

N

R

I

V

V

L

Q

I

L

S

P

A

G

T

M

G

G

P

-

D

-

A

-

S

G

V

V

L

F

K
x
R

I

I

R

A

P

P

L

L

V

T

F

V

G

S

A

E

N

D

D

E

A

I

D

D

R

L

L

G

L

L

T

S

E

L

L

L

active site: W136 (≠ Y154), E208 (≠ D222), D241 (= D255), Q244 (= Q258), K270 (= K285), T301 (= T315), R404 (≠ K416)
binding 1-[((1e)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylene)amino]cyclopentylphosphonic acid: Q50 (≠ V69), M51 (≠ V70), A110 (≠ S128), N113 (= N131), W136 (≠ Y154), H137 (= H155), E208 (≠ D222), S213 (= S227), D241 (= D255), A243 (≠ V257), Q244 (= Q258), K270 (= K285), R404 (≠ K416)

Sites not aligning to the query:

active site: 19

1d7vA Crystal structure of the complex of 2,2-dialkylglycine decarboxylase with nma (see paper)
31% identity, 89% coverage: 42:435/442 of query aligns to 23:423/431 of 1d7vA

query
sites
1d7vA

E

E

P

P

V

M

K

I

I

I

V

E

R

R

G

A

E

K

K

G

V

S

Y

F

L

V

Y

Y

D

D

D

A

Q

D

G

G

N

R

D

A

Y

I

L

L

D

D

A

F

Y

T

N

S

N

G

V

Q

V

M

-

S

-

A

C

V

V

L

G

G

H

H

A

C

N

H

P

P

R

E

I

I

V

V

D

S

A

V

V

I

T

G

R

E

Q

Y

L

A

S

G

T

K

L

L

-

D

C
x
H

T
x
L

H
x
F

T
x
S

R

G

Y

M

M

L

Q

S

E

R

P

P

I

V

L

V

D

D

Y

L

A

A

E

T

D

R

L

L

L

A

S

N

T

I

F

T

N

P

T

P

S

G

I

L

R

D

A

-

G

-

Q

R

M

A

M

L

F

L

T

L

C

S

T

T

G

G

S
x
A

E

E

A

S

N

N

D

E

L

A

A

A

T

I

R

R

I

M

A

A

M

K

Q

L

Y

V

A

T

G

G

K

K

T

Y

G

E

V

I

I

V

V

G

T

F

S

A

E

Q

A

S

Y
x
W

H
|
H

G

G

N

M

S

T

H

G

L

A

T

A

S

A

S

S

F

A

S

T

P

Y

S

S

L

A

G

G

R

R

R

K

A

G

L

V

L

-

G

G

P

P

Y

A

V

A

R

V

-

G

-

S

-

F

T

A

V

I

P

P

A

A

P

P

D

F

S

T

Y

Y

R

R

M

P

T

R

P

F

S

E

E

R

I

N

G

G

Q

A

R

Y

-

D

M

Y

A

L

A

A

Q

E

V

L

A

D

L

Y

Q

A

I

F

E

D

D

L

I

I

-

D

R

R

R

Q

H

S

G

S

G

G

G

N

L

L

A

A

F

F

I

I

A

A

D
x
E

S

P

F

I

F

L

S

S

S
|
S

D

G

G

G

V

I

F

I

A

E

H

L

P

P

T

D

D

G

V

Y

L

M

A

A

P

A

V

L

A

K

E

R

V

K

V

C

R

E

R

A

A

R

G

G

G

M

L

L

F

L

I

I

A

L

D
|
D

E

E

V
x
A

Q
|
Q

S

T

G

G

F

V

G

G

R

R

S

T

G

G

T

T

H

-

M

M

W

F

G

A

H

C

E

Q

R

R

H

D

G

G

V

V

V

T

P

P

D

D

I

I

V

L

T

T

L

L

G

S

K
|
K

P

T

M

L

G

G

N

A

G

G

Y

L

P

P

V

L

A

A

G

A

L

-

V

I

V

V

R

T

P

S

E

A

V

A

V

I

A

E

G

E

F

R

G

A

Q

H

D

E

M

L

R

G

Y

Y

-

L

-

F

F

Y

N

T

T
|
T

F
x
H

G
x
V

G
x
S

N
x
D

S

P

V

L

A

P

I

A

A

A

A

V

A

G

Q

L

A

R

T

V

L

L

D

D

V

V

L

V

R

Q

D

R

E

D

H

G

V

L

L

V

D

A

N

R

A

A

Q

N

R

V

V

M

G

G

A

D

I

R

L

L

A

R

E

R

G

G

L

L

N

L

A

D

L

L

A

M

R

E

K

R

Y

F

E

D

C

C

I

I

G

G

D

D

V

V

R

R

G

G

T

R

G

G

L

L

Y

L

F

L

G

G

V

V

E

E

I

I

V

V

R

K

D

D

R

R

A

R

K

T

K

K

D

E

T

P

D

A

I

D

A

G

T

L

A

G

L

A

K

K

I

I

V

T

N

R

-

E

-

C

-

M

-

N

-

L

G

G

L

L

R

S

Q

M

R

N

R

I

V

V

L

Q

I

L

S

P

A

G

T

M

G

G

P

-

D

-

A

-

S

G

V

V

L

F

K
x
R

I

I

R

A

P

P

L

L

V

T

F

V

G

S

A

E

N

D

D

E

A

I

D

D

R

L

L

G

L

L

T

S

E

L

L

L

active site: W136 (≠ Y154), E208 (≠ D222), D241 (= D255), Q244 (= Q258), K270 (= K285), T301 (= T315), R404 (≠ K416)
binding potassium ion: H75 (≠ C91), L76 (≠ T92), F77 (≠ H93), S78 (≠ T94), T301 (= T315), H302 (≠ F316), V303 (≠ G317), S304 (≠ G318), D305 (≠ N319)
binding n-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-2-methylalanine: A110 (≠ S128), W136 (≠ Y154), H137 (= H155), E208 (≠ D222), S213 (= S227), D241 (= D255), A243 (≠ V257), Q244 (= Q258), K270 (= K285), R404 (≠ K416)

Sites not aligning to the query:

active site: 19

1d7uA Crystal structure of the complex of 2,2-dialkylglycine decarboxylase with lcs (see paper)
31% identity, 89% coverage: 42:435/442 of query aligns to 23:423/431 of 1d7uA

query
sites
1d7uA

E

E

P

P

V

M

K

I

I

I

V

E

R

R

G

A

E

K

K

G

V

S

Y

F

L

V

Y

Y

D

D

D

A

Q

D

G

G

N

R

D

A

Y

I

L

L

D

D

A

F

Y

T

N

S

N

G

V

Q

V

M

-

S

-

A

C

V

V

L

G

G

H

H

A

C

N

H

P

P

R

E

I

I

V

V

D

S

A

V

V

I

T

G

R

E

Q

Y

L

A

S

G

T

K

L

L

-

D

C
x
H

T
x
L

H
x
F

T
x
S

R

G

Y

M

M

L

Q

S

E

R

P

P

I

V

L

V

D

D

Y

L

A

A

E

T

D

R

L

L

L

A

S

N

T

I

F

T

N

P

T

P

S

G

I

L

R

D

A

-

G

-

Q

R

M

A

M

L

F

L

T

L

C

S

T

T

G
|
G

S
x
A

E

E

A

S

N

N

D

E

L

A

A

A

T

I

R

R

I

M

A

A

M

K

Q

L

Y

V

A

T

G

G

K

K

T

Y

G

E

V

I

I

V

V

G

T

F

S

A

E

Q

A

S

Y
x
W

H
|
H

G

G

N

M

S

T

H

G

L

A

T

A

S

A

S

S

F

A

S

T

P

Y

S

S

L

A

G

G

R

R

R

K

A

G

L

V

L

-

G

G

P

P

Y

A

V

A

R

V

-

G

-

S

-

F

T

A

V

I

P

P

A

A

P

P

D

F

S

T

Y

Y

R

R

M

P

T

R

P

F

S

E

E

R

I

N

G

G

Q

A

R

Y

-

D

M

Y

A

L

A

A

Q

E

V

L

A

D

L

Y

Q

A

I

F

E

D

D

L

I

I

-

D

R

R

R

Q

H

S

G

S

G

G

G

N

L

L

A

A

F

F

I

I

A

A

D
x
E

S

P

F

I

F

L

S

S

D

G

G

G

V

I

F

I

A

E

H

L

P

P

T

D

D

G

V

Y

L

M

A

A

P

A

V

L

A

K

E

R

V

K

V

C

R

E

R

A

A

R

G

G

G

M

L

L

F

L

I

I

A

L

D
|
D

E

E

V
x
A

Q
|
Q

S

T

G

G

F

V

G

G

R

R

S

T

G

G

T

T

H

-

M

M

W

F

G

A

H

C

E

Q

R

R

H

D

G

G

V

V

V

T

P

P

D

D

I

I

V

L

T

T

L

L

G

S

K
|
K

P

T

M

L

G

G

N

A

G

G

Y

L

P

P

V

L

A

A

G

A

L

-

V

I

V

V

R

T

P

S

E

A

V

A

V

I

A

E

G

E

F

R

G

A

Q

H

D

E

M

L

R

G

Y

Y

-

L

-

F

F

Y

N

T

T
|
T

F
x
H

G
x
V

G
x
S

N
x
D

S

P

V

L

A

P

I

A

A

A

A

V

A

G

Q

L

A

R

T

V

L

L

D

D

V

V

L

V

R

Q

D

R

E

D

H

G

V

L

L

V

D

A

N

R

A

A

Q

N

R

V

V

M

G

G

A

D

I

R

L

L

A

R

E

R

G

G

L

L

N

L

A

D

L

L

A

M

R

E

K

R

Y

F

E

D

C

C

I

I

G

G

D

D

V

V

R

R

G

G

T

R

G

G

L

L

Y

L

F

L

G

G

V

V

E

E

I

I

V

V

R

K

D

D

R

R

A

R

K

T

K

K

D

E

T

P

D

A

I

D

A

G

T

L

A

G

L

A

K

K

I

I

V

T

N

R

-

E

-

C

-

M

-

N

-

L

G

G

L

L

R

S

Q

M

R

N

R

I

V

V

L

Q

I

L

S

P

A

G

T

M

G

G

P

-

D

-

A

-

S

G

V

V

L

F

K
x
R

I

I

R

A

P

P

L

L

V

T

F

V

G

S

A

E

N

D

D

E

A

I

D

D

R

L

L

G

L

L

T

S

E

L

L

L

active site: W136 (≠ Y154), E208 (≠ D222), D241 (= D255), Q244 (= Q258), K270 (= K285), T301 (= T315), R404 (≠ K416)
binding potassium ion: H75 (≠ C91), L76 (≠ T92), F77 (≠ H93), S78 (≠ T94), T301 (= T315), H302 (≠ F316), V303 (≠ G317), S304 (≠ G318), D305 (≠ N319)
binding [5-hydroxy-6-methyl-4-({[(4E)-3-oxo-1,2-oxazolidin-4-ylidene]amino}methyl)pyridin-3-yl]methyl dihydrogen phosphate: G109 (= G127), A110 (≠ S128), W136 (≠ Y154), H137 (= H155), E208 (≠ D222), D241 (= D255), A243 (≠ V257), Q244 (= Q258), K270 (= K285), R404 (≠ K416)

Sites not aligning to the query:

active site: 19

1d7sA Crystal structure of the complex of 2,2-dialkylglycine decarboxylase with dcs (see paper)
31% identity, 89% coverage: 42:435/442 of query aligns to 23:423/431 of 1d7sA

query
sites
1d7sA

E

E

P

P

V

M

K

I

I

I

V

E

R

R

G

A

E

K

K

G

V

S

Y

F

L

V

Y

Y

D

D

D

A

Q

D

G

G

N

R

D

A

Y

I

L

L

D

D

A

F

Y

T

N

S

N

G

V
x
Q

V

M

-

S

-

A

C

V

V

L

G

G

H

H

A

C

N

H

P

P

R

E

I

I

V

V

D

S

A

V

V

I

T

G

R

E

Q

Y

L

A

S

G

T

K

L

L

-

D

C
x
H

T
x
L

H
x
F

T
x
S

R

G

Y

M

M

L

Q

S

E

R

P

P

I

V

L

V

D

D

Y

L

A

A

E

T

D

R

L

L

L

A

S

N

T

I

F

T

N

P

T

P

S

G

I

L

R

D

A

-

G

-

Q

R

M

A

M

L

F

L

T

L

C

S

T

T

G
|
G

S
x
A

E

E

A

S

N

N

D

E

L

A

A

A

T

I

R

R

I

M

A

A

M

K

Q

L

Y

V

A

T

G

G

K

K

T

Y

G

E

V

I

I

V

V

G

T

F

S

A

E

Q

A

S

Y
x
W

H
|
H

G

G

N

M

S

T

H

G

L

A

T

A

S

A

S

S

F

A

S

T

P

Y

S

S

L

A

G

G

R

R

R

K

A

G

L

V

L

-

G

G

P

P

Y

A

V

A

R

V

-

G

-

S

-

F

T

A

V

I

P

P

A

A

P

P

D

F

S

T

Y

Y

R

R

M

P

T

R

P

F

S

E

E

R

I

N

G

G

Q

A

R

Y

-

D

M

Y

A

L

A

A

Q

E

V

L

A

D

L

Y

Q

A

I

F

E

D

D

L

I

I

-

D

R

R

R

Q

H

S

G

S

G

G

G

N

L

L

A

A

F

F

I

I

A

A

D
x
E

S

P

F

I

F

L

S

S

D

G

G

G

V

I

F

I

A

E

H

L

P

P

T

D

D

G

V

Y

L

M

A

A

P

A

V

L

A

K

E

R

V

K

V

C

R

E

R

A

A

R

G

G

G

M

L

L

F

L

I

I

A

L

D
|
D

E

E

V
x
A

Q
|
Q

S

T

G

G

F

V

G

G

R

R

S

T

G

G

T

T

H

-

M

M

W

F

G

A

H

C

E

Q

R

R

H

D

G

G

V

V

V

T

P

P

D

D

I

I

V

L

T

T

L

L

G

S

K
|
K

P

T

M

L

G

G

N

A

G

G

Y

L

P

P

V

L

A

A

G

A

L

-

V

I

V

V

R

T

P

S

E

A

V

A

V

I

A

E

G

E

F

R

G

A

Q

H

D

E

M

L

R

G

Y

Y

-

L

-

F

F

Y

N

T

T
|
T

F
x
H

G
x
V

G
x
S

N
x
D

S

P

V

L

A

P

I

A

A

A

A

V

A

G

Q

L

A

R

T

V

L

L

D

D

V

V

L

V

R

Q

D

R

E

D

H

G

V

L

L

V

D

A

N

R

A

A

Q

N

R

V

V

M

G

G

A

D

I

R

L

L

A

R

E

R

G

G

L

L

N

L

A

D

L

L

A

M

R

E

K

R

Y

F

E

D

C

C

I

I

G

G

D

D

V

V

R

R

G

G

T

R

G

G

L

L

Y

L

F

L

G

G

V

V

E

E

I

I

V

V

R

K

D

D

R

R

A

R

K

T

K

K

D

E

T

P

D

A

I

D

A

G

T

L

A

G

L

A

K

K

I

I

V

T

N

R

-

E

-

C

-

M

-

N

-

L

G

G

L

L

R

S

Q

M

R

N

R

I

V

V

L

Q

I

L

S

P

A

G

T

M

G

G

P

-

D

-

A

-

S

G

V

V

L

F

K
x
R

I

I

R

A

P

P

L

L

V

T

F

V

G

S

A

E

N

D

D

E

A

I

D

D

R

L

L

G

L

L

T

S

E

L

L

L

active site: W136 (≠ Y154), E208 (≠ D222), D241 (= D255), Q244 (= Q258), K270 (= K285), T301 (= T315), R404 (≠ K416)
binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: Q50 (≠ V69), G109 (= G127), A110 (≠ S128), W136 (≠ Y154), H137 (= H155), E208 (≠ D222), D241 (= D255), A243 (≠ V257), Q244 (= Q258), K270 (= K285), R404 (≠ K416)
binding potassium ion: H75 (≠ C91), L76 (≠ T92), F77 (≠ H93), S78 (≠ T94), T301 (= T315), H302 (≠ F316), V303 (≠ G317), S304 (≠ G318), D305 (≠ N319)

Sites not aligning to the query:

active site: 19

1d7rA Crystal structure of the complex of 2,2-dialkylglycine decarboxylase with 5pa (see paper)
31% identity, 89% coverage: 42:435/442 of query aligns to 23:423/431 of 1d7rA

query
sites
1d7rA

E

E

P

P

V

M

K

I

I

I

V

E

R

R

G

A

E

K

K

G

V

S

Y

F

L

V

Y

Y

D

D

D

A

Q

D

G

G

N

R

D

A

Y

I

L

L

D

D

A

F

Y

T

N

S

N

G

V

Q

V

M

-

S

-

A

C

V

V

L

G

G

H

H

A

C

N

H

P

P

R

E

I

I

V

V

D

S

A

V

V

I

T

G

R

E

Q

Y

L

A

S

G

T

K

L

L

-

D

C
x
H

T
x
L

H
x
F

T
x
S

R

G

Y

M

M

L

Q

S

E

R

P

P

I

V

L

V

D

D

Y

L

A

A

E

T

D

R

L

L

L

A

S

N

T

I

F

T

N

P

T

P

S

G

I

L

R

D

A

-

G

-

Q

R

M

A

M

L

F

L

T

L

C

S

T

T

G
|
G

S
x
A

E

E

A

S

N

N

D

E

L

A

A

A

T

I

R

R

I

M

A

A

M

K

Q

L

Y

V

A

T

G

G

K

K

T

Y

G

E

V

I

I

V

V

G

T

F

S

A

E

Q

A

S

Y
x
W

H
|
H

G

G

N

M

S

T

H

G

L

A

T

A

S

A

S

S

F

A

S

T

P

Y

S

S

L

A

G

G

R

R

R

K

A

G

L

V

L

-

G

G

P

P

Y

A

V

A

R

V

-

G

-

S

-

F

T

A

V

I

P

P

A

A

P

P

D

F

S

T

Y

Y

R

R

M

P

T

R

P

F

S

E

E

R

I

N

G

G

Q

A

R

Y

-

D

M

Y

A

L

A

A

Q

E

V

L

A

D

L

Y

Q

A

I

F

E

D

D

L

I

I

-

D

R

R

R

Q

H

S

G

S

G

G

G

N

L

L

A

A

F

F

I

I

A

A

D
x
E

S

P

F

I

F

L

S

S

S
|
S

D

G

G

G

V

I

F

I

A

E

H

L

P

P

T

D

D

G

V

Y

L

M

A

A

P

A

V

L

A

K

E

R

V

K

V

C

R

E

R

A

A

R

G

G

G

M

L

L

F

L

I

I

A

L

D
|
D

E

E

V
x
A

Q
|
Q

S

T

G

G

F

V

G

G

R

R

S

T

G

G

T

T

H

-

M

M

W

F

G

A

H

C

E

Q

R

R

H

D

G

G

V

V

V

T

P

P

D

D

I

I

V

L

T

T

L

L

G

S

K
|
K

P

T

M

L

G

G

N

A

G

G

Y

L

P

P

V

L

A

A

G

A

L

-

V

I

V

V

R

T

P

S

E

A

V

A

V

I

A

E

G

E

F

R

G

A

Q

H

D

E

M

L

R

G

Y

Y

-

L

-

F

F

Y

N

T

T
|
T

F
x
H

G
x
V

G
x
S

N
x
D

S

P

V

L

A

P

I

A

A

A

A

V

A

G

Q

L

A

R

T

V

L

L

D

D

V

V

L

V

R

Q

D

R

E

D

H

G

V

L

L

V

D

A

N

R

A

A

Q

N

R

V

V

M

G

G

A

D

I

R

L

L

A

R

E

R

G

G

L

L

N

L

A

D

L

L

A

M

R

E

K

R

Y

F

E

D

C

C

I

I

G

G

D

D

V

V

R

R

G

G

T

R

G

G

L

L

Y

L

F

L

G

G

V

V

E

E

I

I

V

V

R

K

D

D

R

R

A

R

K

T

K

K

D

E

T

P

D

A

I

D

A

G

T

L

A

G

L

A

K

K

I

I

V

T

N

R

-

E

-

C

-

M

-

N

-

L

G

G

L

L

R

S

Q

M

R

N

R

I

V

V

L

Q

I

L

S

P

A

G

T

M

G

G

P

-

D

-

A

-

S

G

V

V

L

F

K
x
R

I

I

R

A

P

P

L

L

V

T

F

V

G

S

A

E

N

D

D

E

A

I

D

D

R

L

L

G

L

L

T

S

E

L

L

L

active site: W136 (≠ Y154), E208 (≠ D222), D241 (= D255), Q244 (= Q258), K270 (= K285), T301 (= T315), R404 (≠ K416)
binding n-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-y-lmethyl]-1-amino-cyclopropanecarboxylic acid: G109 (= G127), A110 (≠ S128), W136 (≠ Y154), H137 (= H155), E208 (≠ D222), S213 (= S227), D241 (= D255), A243 (≠ V257), Q244 (= Q258), K270 (= K285), R404 (≠ K416)
binding potassium ion: H75 (≠ C91), L76 (≠ T92), F77 (≠ H93), S78 (≠ T94), T301 (= T315), H302 (≠ F316), V303 (≠ G317), S304 (≠ G318), D305 (≠ N319)

Sites not aligning to the query:

active site: 19

Query Sequence

>BPHYT_RS29280 FitnessBrowser__BFirm:BPHYT_RS29280
MRGDMPNGFDPASLDHLDAGTQRHIARRLRLLSPSYRLFYAEPVKIVRGEKVYLYDDQGN
DYLDAYNNVVCVGHANPRIVDAVTRQLSTLCTHTRYMQEPILDYAEDLLSTFNTSIRAGQ
MMFTCTGSEANDLATRIAMQYAGKTGVIVTSEAYHGNSHLTSSFSPSLGRRALLGPYVRT
VPAPDSYRMTPSEIGQRMAAQVALQIEDIRRHGGGLAAFIADSFFSSDGVFAHPTDVLAP
VAEVVRRAGGLFIADEVQSGFGRSGTHMWGHERHGVVPDIVTLGKPMGNGYPVAGLVVRP
EVVAGFGQDMRYFNTFGGNSVAIAAAQATLDVLRDEHVLDNAQRVGAILAEGLNALARKY
ECIGDVRGTGLYFGVEIVRDRAKKDTDIATALKIVNGLRQRRVLISATGPDASVLKIRPP
LVFGANDADRLLTELDTVLAAL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory