SitesBLAST

Q94JQ4 Reactive Intermediate Deaminase A, chloroplastic; 2-iminobutanoate/2-iminopropanoate deaminase; EC 3.5.99.10 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
38% identity, 83% coverage: 24:134/134 of query aligns to 77:187/187 of Q94JQ4

query
sites
Q94JQ4

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R165 (= R112) mutation to A: Loss of activity.

5hp8A Crystal structures of rida in complex with pyruvate (see paper)
38% identity, 77% coverage: 32:134/134 of query aligns to 6:108/108 of 5hp8A

query
sites
5hp8A

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binding pyruvic acid: R86 (= R112), T88 (≠ V114)

P52759 2-iminobutanoate/2-iminopropanoate deaminase; Liver perchloric acid-soluble protein; L-PSP; Reactive intermediate imine deaminase A homolog; Translation inhibitor L-PSP ribonuclease; UK114 antigen homolog; rp14.5; EC 3.5.99.10 from Rattus norvegicus (Rat) (see paper)
39% identity, 81% coverage: 24:132/134 of query aligns to 19:127/137 of P52759

query
sites
P52759

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A

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

5v4dA Crystal structure of the protein of unknown function of the conserved rid protein family yyfa from yersinia pestis
38% identity, 83% coverage: 24:134/134 of query aligns to 16:127/127 of 5v4dA

query
sites
5v4dA

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binding calcium ion: D91 (≠ E99), D95 (≠ R103)

7cd4A Crystal structure of the s103f mutant of bacillus subtilis (natto) yabj protein. (see paper)
36% identity, 83% coverage: 24:134/134 of query aligns to 14:123/124 of 7cd4A

query
sites
7cd4A

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binding zinc ion: E47 (≠ A58)

Sites not aligning to the query:

binding zinc ion: 5, 8

3k0tC Crystal structure of pspto -psp protein in complex with d-beta-glucose from pseudomonas syringae pv. Tomato str. Dc3000 (see paper)
31% identity, 96% coverage: 7:134/134 of query aligns to 6:123/124 of 3k0tC

query
sites
3k0tC

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binding beta-D-glucopyranose: Y16 (= Y26), G30 (= G40), I32 (≠ L42), F83 (≠ W94), R101 (= R112), R111 (≠ F122), E116 (= E127)

P80601 2-iminobutanoate/2-iminopropanoate deaminase; 14.3 kDa perchloric acid soluble protein; Translation inhibitor L-PSP ribonuclease; UK114 antigen; EC 3.5.99.10; EC 3.1.-.- from Capra hircus (Goat) (see paper)
34% identity, 81% coverage: 24:132/134 of query aligns to 19:127/137 of P80601

query
sites
P80601

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Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

Q7CP78 2-iminobutanoate/2-iminopropanoate deaminase; Enamine/imine deaminase; EC 3.5.99.10 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
38% identity, 90% coverage: 12:132/134 of query aligns to 3:125/128 of Q7CP78

query
sites
Q7CP78

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Y17 (= Y26) mutation to F: Deaminase activity is defective. At pH 8, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.
R105 (= R112) mutation to A: Deaminase activity is defective. At pH 8 and 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.
E120 (= E127) Important for catalytic activity at high pH; mutation to A: Deaminase activity is defective. At pH 8, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.; mutation to K: Deaminase activity is defective. At pH 8, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.

2uynA Crystal structure of e. Coli tdcf with bound 2-ketobutyrate (see paper)
36% identity, 74% coverage: 34:132/134 of query aligns to 24:124/127 of 2uynA

query
sites
2uynA

G

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binding 2-ketobutyric acid: F83 (≠ W94), R104 (= R112), S105 (≠ A113), C106 (≠ V114)

2uykC Crystal structure of e. Coli tdcf with bound serine (see paper)
36% identity, 74% coverage: 34:132/134 of query aligns to 24:124/127 of 2uykC

query
sites
2uykC

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A

A

binding serine: R104 (= R112), S105 (≠ A113), C106 (≠ V114)

3vczB 1.80 angstrom resolution crystal structure of a putative translation initiation inhibitor from vibrio vulnificus cmcp6
35% identity, 82% coverage: 23:132/134 of query aligns to 14:125/127 of 3vczB

query
sites
3vczB

G

G

G

P

H

Y

Y

I

S

Q

H

G

V

V

S

D

I

L

A

G

N

N

G

-

M

M

V

V

F

L

V

T

S

S

G

G

Q

Q

L

I

P

P

I

V

D

N

P

P

-

A

T

T

G

G

R

E

K

V

L

P

T

A

D

D

Q

-

P

-

F

I

E

A

A

A

Q

Q

A

A

E

R

Q

Q

V

S

L

L

A

D

N

N

L

V

A

K

T

A

A

V

L

V

A

E

G

A

A

S

G

G

S

L

D

T

V

V

T

G

K

D

L

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V

V

Q

K

V

M

R

T

V

V

Y

F

I

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V

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D
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D

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L

E
x
N

Y

D

W

F

A

G

T

T

F

V

N

N

E

E

I

V

Y

Y

A

G

R

N

W

F

T

F

G

D

-

E

-

H

-

N

-

V

A

A

A

H

K

Y

P

P

A

A

R

R

A

S

V

C

V

V

P

E

V

V

P

A

A

R

L

L

H

P

F

K

G

D

L

V

K

G

I

I

E

E

V

I

E

E

A

A

V

I

A

A

binding magnesium ion: D79 (= D90), N81 (≠ E92)

A0A1J1DL12 2-iminobutanoate/2-iminopropanoate deaminase; Allergen Der f 34; Enamine/imine deaminase; Allergen Der f 34.0101; EC 3.5.99.10 from Dermatophagoides farinae (American house dust mite) (see paper)
24% identity, 93% coverage: 10:134/134 of query aligns to 2:127/128 of A0A1J1DL12

query
sites
A0A1J1DL12

C

S

P

P

R

K

T

R

I

I

N

I

P

S

E

T

G

P

L

L

A

A

P

P

-

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P

P

G

I

G

G

H

P

Y
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Y

S

S

H

Q

V

A

S

V

I

Q

A

V

N

G

G

N

M

T

V

V

F

Y

V

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S

S

G
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G

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Q

L

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G

I

M

D

N

P

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T

R

G

T

R

N

K

E

L

M

T

V

D

T

Q

G

P

P

F

I

E

R

A

D

Q

E

A

A

E

Q

Q

Q

V

A

L

F

A

T

N
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N

L

M

A

K

T

A

A

V

L

V

A

E

G

A

A

S

G

G

S

A

D

K

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M

T

S

K

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L

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V

V

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K

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V

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Y

F

I

I

V

R

D

N

I

F

E

N

Y

D

W

F

A

P

T

A

F

I

N
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N

E

D

I

V

Y

M

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K

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E

W

F

T

F

G

Q

A

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A

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K

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A

R
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R

A

S

V

T

V

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G

V

V

P

A

A

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L

L

H
x
P

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K

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E
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E

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E

A

S

V

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A

V

C

V

V

I

Y19 (= Y26) mutation to A: Loss of imine deaminase activity; when associated with A-33; A-59; A-91; A-105; A-114 and A-120.
G33 (= G40) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-59; A-91; A-105; A-114 and A-120.
N59 (= N66) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-91; A-105; A-114 and A-120.
N91 (= N98) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-105; A-114 and A-120.
R105 (= R112) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-91; A-114 and A-120.
P114 (≠ H121) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-91; A-105 and A-120.
E120 (= E127) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-91; A-105 and A-114.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P0AFQ5 3-aminoacrylate deaminase RutC; 3-AA deaminase; EC 3.5.-.- from Escherichia coli (strain K12) (see paper)
30% identity, 76% coverage: 14:115/134 of query aligns to 6:107/128 of P0AFQ5

query
sites
P0AFQ5

I

I

N

I

P

P

E

A

G

G

L

S

A

S

P

A

P

P

G

L

G

A

H

P

Y

F

S

V

H

P

V

G

S

T

I

L

A

A

N

D

G

G

M

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V

V

F

Y

V

V

S

S

G

G

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T

L

L

P

A

I

F

D

D

P

Q

T

H

G

N

R

N

K

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L

T

F

D

A

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D

P

D

F

P

E

K

A

A

Q

Q

A

T

E

R

Q

H

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V

L

L

A

E

N

T

L

I

A

R

T

K

A

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L

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A

E

G

T

A

A

G

G

S

G

D

T

V

M

T

A

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D

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V

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F

V

N

R

S

V

I

Y

F

I

I

V

T

D

D

I

W

E

K

Y

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W

Y

A

A

T

A

F

I

N

N

E

E

I

I

Y

Y

A

A

R

E

W

F

T

F

G

P

A

G

A

D

K

K

P

P

A

A

R
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R

A

F

V

C

V

I

R104 (= R112) mutation to A: Loss of activity.

Query Sequence

>BPHYT_RS29300 FitnessBrowser__BFirm:BPHYT_RS29300
MDKRERSEQCPRTINPEGLAPPGGHYSHVSIANGMVFVSGQLPIDPTGRKLTDQPFEAQA
EQVLANLATALAGAGSDVTKLVQVRVYIVDIEYWATFNEIYARWTGAAKPARAVVPVPAL
HFGLKIEVEAVACV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory