SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS31070 FitnessBrowser__BFirm:BPHYT_RS31070 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
34% identity, 62% coverage: 59:191/213 of query aligns to 62:192/203 of P07464

query
sites
P07464

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S71 (= S68) binding
N85 (≠ G82) binding in other chain; binding in other chain
D93 (≠ T90) binding
H115 (= H112) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ E142) binding in other chain
A160 (= A159) binding in other chain
TK 165:166 (≠ TS 164:165) binding
R180 (= R179) binding
R183 (= R182) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
34% identity, 62% coverage: 59:191/213 of query aligns to 61:191/201 of 1krvA

query
sites
1krvA

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binding 4-nitrophenyl beta-D-galactopyranoside: S70 (= S68), Y82 (= Y80), N84 (≠ G82), V90 (≠ I88), D92 (≠ T90), M126 (≠ I127)
binding coenzyme a: A104 (= A102), S110 (≠ A108), T112 (≠ Y110), W138 (= W139), G140 (= G141), S141 (≠ E142), N146 (vs. gap), A159 (= A159), P177 (= P177), R179 (= R179), R182 (= R182)

Sites not aligning to the query:

binding 4-nitrophenyl beta-D-galactopyranoside: 16, 25

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
34% identity, 62% coverage: 59:191/213 of query aligns to 61:191/201 of 1kruA

query
sites
1kruA

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binding coenzyme a: H114 (= H112), W138 (= W139), G140 (= G141), S141 (≠ E142), G158 (= G158), A159 (= A159), P177 (= P177), R182 (= R182)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: W62 (= W60), S70 (= S68), Y82 (= Y80), Y82 (= Y80), N84 (≠ G82), V90 (≠ I88), D92 (≠ T90), L102 (= L100), H114 (= H112), M126 (≠ I127)

Sites not aligning to the query:

binding 1-methylethyl 1-thio-beta-D-galactopyranoside: 16, 22, 25

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
34% identity, 62% coverage: 59:191/213 of query aligns to 61:191/200 of 1krrA

query
sites
1krrA

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binding acetyl coenzyme *a: N84 (≠ G82), I103 (= I101), A104 (= A102), P105 (≠ D103), T112 (≠ Y110), H114 (= H112), G140 (= G141), S141 (≠ E142), N146 (vs. gap), G158 (= G158), A159 (= A159), A174 (≠ V174), P177 (= P177), R182 (= R182)

4isxA The crystal structure of maltose o-acetyltransferase from clostridium difficile 630 in complex with acetyl-coa
31% identity, 75% coverage: 25:183/213 of query aligns to 53:183/186 of 4isxA

query
sites
4isxA

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binding acetyl coenzyme *a: N84 (= N56), A104 (= A102), W138 (= W139), G140 (= G141), G141 (≠ E142), A159 (= A159), V174 (= V174), K182 (≠ R182)

5u2kA Crystal structure of galactoside o-acetyltransferase complex with coa (h3 space group)
28% identity, 71% coverage: 32:182/213 of query aligns to 46:182/190 of 5u2kA

query
sites
5u2kA

R

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K

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binding coenzyme a: M90 (≠ I88), Y110 (≠ A108), A112 (≠ Y110), H114 (= H112), L146 (= L147), T164 (= T164), K165 (≠ S165)

3mqhA Crystal structure of the 3-n-acetyl transferase wlbb from bordetella petrii in complex with coa and udp-3-amino-2-acetamido-2,3-dideoxy glucuronic acid (see paper)
30% identity, 73% coverage: 27:181/213 of query aligns to 15:152/191 of 3mqhA

query
sites
3mqhA

A

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A
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K

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I

binding coenzyme a: F76 (≠ Y106), T84 (≠ E115), N85 (≠ D116), V86 (≠ I117), Y87 (≠ H118), P89 (= P120), G111 (= G141), A112 (≠ E142), A130 (= A159), N135 (≠ T164), V145 (= V174), P148 (= P177), R150 (= R179)
binding (2S,3S,4R,5R,6R)-5-(acetylamino)-4-amino-6-{[(R)-{[(R)-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}-3-hydroxytetrahydro-2H-pyran-2-carboxylic acid: W24 (≠ L36), H28 (≠ R40), F46 (= F58), Q60 (≠ T90), N61 (≠ H91), Y66 (≠ G96), F76 (≠ Y106), N85 (≠ D116), V86 (≠ I117), R95 (≠ D125), Y99 (≠ R129)

Sites not aligning to the query:

binding coenzyme a: 161

3mqgC Crystal structure of the 3-n-acetyl transferase wlbb from bordetella petrii in complex with acetyl-coa (see paper)
29% identity, 73% coverage: 27:181/213 of query aligns to 16:153/192 of 3mqgC

query
sites
3mqgC

A

A

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R

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G

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A

N

E

I

I

A

G

I

E

G

G

T

C

D
x
S

V

L

F

G

V

Q

N

N

N

V

F
|
F

A

-

W

-

I

-

E

-

T

-

I

-

R

-

A

-

F

-

S

-

V

-

Q

-

P

-

R

-

L

-

D

-

I

V

R

G

D

N

R

R

V

V

Y

R

I

I

G

G

N

N

S

-

A

-

H

R

I

V

I

K

V

I

T

Q

H
x
N

S

N

I

V

E

S

I

V

G
x
Y

C

D

D

N

V

V

L

F

I

L

A

E

D

D

R

D

V

V

Y
x
F

V

C

A

G

D
x
P

Y

S

I

M

H

V

G

-

Y

F

E
x
T

D
x
N

I
x
V

H
x
Y

T

N

P
|
P

-

R

V

A

K

A

T

I

Q

E

D

R

L

K

I

S

Q

E

R

Y

R

R

P

D

V

T

N

I

I

V

G

R

D

Q

G

G

S

A

W

T

I

L

G
|
G

E
x
A

N

N

-

C

V

T

V

V

I

V

L

C

G

G

A

A

R

T

I

I

G

G

R

R

H

Y

C

A

V
x
F

I

V

G
|
G

A
|
A

N

G

A

A

V

V

T
x
N

S

K

D

D

I

V

P

P

D

D

F

F

C

A

V

L

A

V

V
|
V

G

G

A

V

P
|
P

A

A

R
|
R

V

Q

V

I

binding acetyl coenzyme *a: Y67 (≠ G96), F77 (≠ Y106), P80 (≠ D109), T85 (≠ E115), N86 (≠ D116), V87 (≠ I117), Y88 (≠ H118), P90 (= P120), G112 (= G141), A113 (≠ E142), F128 (≠ V156), G130 (= G158), A131 (= A159), N136 (≠ T164), V146 (= V174), P149 (= P177), R151 (= R179)
binding uridine-5'-monophosphate: H29 (≠ R40), F47 (= F58)
binding uridine-5'-diphosphate: W25 (≠ L36), S41 (≠ D52), N62 (≠ H91)

Sites not aligning to the query:

binding acetyl coenzyme *a: 162

3igjC Crystal structure of maltose o-acetyltransferase complexed with acetyl coenzyme a from bacillus anthracis
37% identity, 40% coverage: 97:182/213 of query aligns to 95:184/188 of 3igjC

query
sites
3igjC

C

C

D

E

V

V

L

R

I

I

A

G

D

D

R

H

V

C

Y

M

V

F

A
|
A

D

P

Y

G

I

V

H

H

G

I

Y
|
Y

E

T

D
x
A

I

T

H
|
H

T

P

-

L

-

H

P

P

V

V

K

E

T

R

Q

N

D

S

L

G

I

K

Q

E

R

Y

-

G

R

K

P

P

V

V

N

K

I

I

G

G

D

N

G

N

S

V

W

W

I

V

G
|
G

E

G

N

G

V

A

V

I

I

I

-
x
N

L

P

G

G

A

V

R

S

I

I

G

G

R

D

H

N

C

A

V

V

I

I

G
x
A

A
x
S

N

G

A

A

V

V

T
|
T

S

K

D

D

I

V

P

P

D

N

F

N

C

V

V

V

A

V

V

G

G

G

A
x
N

P

P

A

A

R

K

V

V

V

I

R

K

binding acetyl coenzyme *a: A106 (= A108), Y112 (= Y114), A114 (≠ D116), H116 (= H118), G142 (= G141), N148 (vs. gap), A160 (≠ G158), S161 (≠ A159), T166 (= T164), N178 (≠ A176)

Sites not aligning to the query:

binding acetyl coenzyme *a: 84

A1ADJ6 Polysialic acid O-acetyltransferase; Capsule O-acetyl transferase; EC 2.3.1.136 from Escherichia coli O1:K1 / APEC (see paper)
32% identity, 49% coverage: 78:182/213 of query aligns to 174:278/307 of A1ADJ6

query
sites
A1ADJ6

R

R

V

T

Y

T

I

I

G

G

N

A

S

G

A

F

H

E

I

V

V

T

T

D

H

K

-

C

S

N

I

V

E

T

I

I

G

G

C

H

D

D

V

C

L

M

I

I

A

A

-

R

D

D

R

V

V

I

Y

L

V

R

A

A

D

S

Y

D

I

G

H
|
H

G

P

Y

I

E

F

D

D

I

I

H

H

T

S

P

K

V

K

K

R

-

I

-

N

-

W

T

A

Q

K

D

D

L

I

I

I

Q

-

R

-

P

-

V

-

N

-

I

I

G

S

D

S

G

Y

S

V

W
|
W

I

V

G

G

E

R

N

N

V

V

V

S

I

I

L

M

-

K

G

G

A

V

R

S

I

V

G

G

R

S

H

G

C

S

V

V

I

I

G

G

A

Y

N

G

A

S

V

I

V

V

T

T

S

K

D

D

I

V

P

P

D

S

F

M

C

C

V

A

A

A

V

A

G

G

A

N

P

P

A

A

R

K

V

I

R

K

H210 (= H112) mutation to A: Loss of activity.
W234 (= W139) mutation to A: Loss of activity.

G3XD01 UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronate N-acetyltransferase; UDP-D-GlcNAc3NA N-acetyltransferase; UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronic acid 3-N-acetyltransferase; EC 2.3.1.201 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
25% identity, 76% coverage: 45:205/213 of query aligns to 13:175/191 of G3XD01

query
sites
G3XD01

D

D

N

G

I

A

A

Q

I

I

G

G

T

S

D

D

V

S

F

R

V

V

N

W

N

H

F

F

A

V

W

H

I

I

E

-

T

-

I

C

R

A

A

G

F

A

S

R

V

I

Q

G

P

A

R

G

L

V

D

S

I

L

R

G

D

Q

R

N

V

V

Y

F

I

V

G

G

N

N

S

K

A

-

H

-

I

V

V

I

T

G

H

D

S

R

I

C

E

K

I

I

G

Q

C

N

D

N

V

V

L

S

I

V

A

Y

D

D

R

N

V

V

Y

T

V

L

A

E

D

E

Y

G

I

V

H

F

G

C

-

G

-

P

-

S

-

M

-

V

Y

F

E

T

D

N

I

V

H

Y

T

N

P

P

V

-

K

-

T

-

Q

R

D

S

L

L

I

I

Q

E

R

R

-

K

-

D

-

Q

-

Y

R

R

P

N

V

T

N

L

I

V

G

K

D

K

G

G

S

A

W

T

I

L

G

G

E

A

N

N

V

C

V

T

I

I

L

V

-

C

G

G

A

V

R

T

I

I

G

G

R

E

H

Y

C

A

V

F

I

V

G

G

A

A

N

G

A

A

V

V

V

I

T

N

S
x
K

D

N

I

V

P

P

D

S

F

Y

C

A

V

L

A

M

V

V

G

G

A

V

P

P

A

A

R

R

V

Q

V

I

R

-

H

G

W

W

H

M

P

S

D

E

R

F

K

G

Q

E

W

Q

L

L

K

Q

G

L

A

N

P

E

S

Q

A

G

R

E

A

A

A

V

C

C

S

S

A

H

S

S

K136 (≠ S165) mutation to A: Reduction in catalytic activity.; mutation to R: No effect on catalytic activity.

7zw9A Crystal structure of a gamma-carbonic anhydrase from the pathogenic bacterium burkholderia pseudomallei (see paper)
53% identity, 23% coverage: 133:182/213 of query aligns to 90:142/173 of 7zw9A

query
sites
7zw9A

N

T

I

I

G

G

D

E

G

G

S

S

W

L

I

I

G

G

E

I

N

Q

V

A

V

V

I

I

L

L

G

N

-

R

A

A

R

V

I

I

G

G

R

R

H

N

C

C

V

L

I

V

G

G

A

A

N

G

A

A

V

V

V

I

T

T

S

E

D

G

-

K

-

A

I

F

P

P

D

D

F

N

C

S

V

L

A

I

V

L

G

G

A

A

P

P

A

A

R

K

V

V

R

R

Sites not aligning to the query:

binding zinc ion: 65, 87

3nz2J Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
46% identity, 25% coverage: 130:182/213 of query aligns to 129:182/185 of 3nz2J

query
sites
3nz2J

R

K

P

P

V

I

N

V

I

I

G

E

D

D

G

D

S

V

W
|
W

I

I

G
|
G

E

G

N

N

V

V

I

I

-

N

L

Q

G

G

A

V

R

T

I

I

G

G

R

A

H

R

C

S

V

V

I

V

G
x
A

A
|
A

N

N

A

S

V

V

T
x
N

S

Q

D

D

I

V

P

P

D

P

F

D

C

T

V

L

A

V

V
x
G

G

G

A
x
T

P
|
P

A

A

R

R

V

I

V

L

R
|
R

binding acetyl coenzyme *a: W138 (= W139), G140 (= G141), A158 (≠ G158), A159 (= A159), N164 (≠ T164), G174 (≠ V174), T176 (≠ A176), P177 (= P177), R182 (= R182)

Sites not aligning to the query:

binding acetyl coenzyme *a: 104, 110, 114

3nz2C Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
46% identity, 25% coverage: 130:182/213 of query aligns to 126:179/183 of 3nz2C

query
sites
3nz2C

R

K

P

P

V

I

N

V

I

I

G

E

D

D

G

D

S

V

W

W

I

I

G
|
G

E

G

N

N

V

V

I

I

-

N

L

Q

G

G

A

V

R

T

I

I

G

G

R

A

H

R

C

S

V

V

I

V

G
x
A

A
|
A

N
|
N

A

S

V

V

T
x
N

S

Q

D

D

I

V

P

P

D

P

F

D

C

T

V

L

A

V

V
x
G

G

G

A
x
T

P
|
P

A

A

R

R

V

I

V
x
L

R
|
R

binding acetyl coenzyme *a: G137 (= G141), A155 (≠ G158), A156 (= A159), N161 (≠ T164), G171 (≠ V174), T173 (≠ A176), P174 (= P177), L178 (≠ V181), R179 (= R182)
binding magnesium ion: N157 (= N160), T173 (≠ A176)

Sites not aligning to the query:

binding acetyl coenzyme *a: 107, 111

3ectA Crystal structure of the hexapeptide-repeat containing- acetyltransferase vca0836 from vibrio cholerae
46% identity, 25% coverage: 130:182/213 of query aligns to 119:172/176 of 3ectA

query
sites
3ectA

R

K

P

P

V

I

N

V

I

I

G

E

D

D

G

D

S

V

W

W

I

I

G

G

E

G

N

N

V

V

I

I

-

N

L

Q

G
|
G

A

V

R

T

I

I

G

G

R

A

H

R

C

S

V

V

I

V

G

A

A

A

N

N

A

S

V

V

T

N

S

Q

D
|
D

I

V

P

P

D

P

F

D

C

T

V

L

A

V

V

G

G

G

A

T

P

P

A

A

R

R

V

I

V

L

R

R

binding calcium ion: G138 (= G148), D156 (= D166)

6wyeA Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) (see paper)
52% identity, 25% coverage: 129:181/213 of query aligns to 193:245/261 of 6wyeA

query
sites
6wyeA

R
|
R

R
x
H

P

P

V

-

N

K

I

I

G

G

D

D

G

G

S

V

W

M

I

I

G

G

E

A

N

N

V

A

V

S

I

I

L

L

G

G

A

N

-

I

R

R

I

I

G

G

R

S

H

N

C

A

V

K

I

I

G

G

A

A

N

G

A

S

V

V

T

V

S

S

D

D

I

V

P

P

D

P

F

S

C

I

V

T

A

V

V

V

G

G

A

V

P

P

A

A

R

K

V

P

V

V

binding (2S)-2-hydroxybutanedioic acid: R193 (= R129), H194 (≠ R130)

Sites not aligning to the query:

binding (2S)-2-hydroxybutanedioic acid: 93, 94, 158, 159, 185

4mzuB Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
29% identity, 46% coverage: 85:183/213 of query aligns to 39:142/290 of 4mzuB

query
sites
4mzuB

A
|
A

H

N

I

S

I

L

V

I

T

E

H

N

S

D

I

V

E

V

I

I

G

G

C

D

D

N

V

V

L

T

I

I

A

K

D

S

R
x
G

V

V

Y

Q

V

I

A

W

D

D

Y

G

I

I

H

H

G

I

Y

Q

E

D

D

D

I

V

H
x
F

T

I

P

G

V

P

K
x
N

T

V

Q

T

D

F

L
x
T

I
x
N

Q
x
D

R
x
K

R

Q

P
|
P

V

R

N

S

-

L

-

K

-

T

-

I

I

V

G

K

D

K

G

G

S

A

W

S

I

I

G
|
G

E
x
A

N

N

V

S

V

T

I

I

L

L

-

P

G

G

A

I

R

L

I

I

G

G

R

E

H

N

C

A

V

M

I

V

G
|
G

A
|
A

N

G

A

A

V

V

V

I

T
|
T

S
x
K

D

N

I

V

P

P

D

D

F

N

C

A

V

I

A

V

V
x
I

G

G

A

N

P

P

A

G

R
|
R

V

I

V

T

R

G

H

Y

binding coenzyme a: F72 (≠ H118), T80 (≠ L126), N81 (≠ I127), D82 (≠ Q128), K83 (≠ R129), P85 (= P131), G99 (= G141), A100 (≠ E142), G117 (= G158), A118 (= A159), T123 (= T164), K124 (≠ S165), I133 (≠ V174), R138 (= R179)
binding magnesium ion: G58 (≠ R104), N76 (≠ K122)
binding thymine: A39 (= A85)

Sites not aligning to the query:

binding coenzyme a: 199
binding thymine: 20, 21, 38
binding thymidine-5'-diphosphate: 171, 176, 189, 191, 202, 203, 270

2fkoA Structure of ph1591 from pyrococcus horikoshii ot3 (see paper)
31% identity, 30% coverage: 131:193/213 of query aligns to 71:153/173 of 2fkoA

query
sites
2fkoA

P

P

V

T

N

E

I

I

G

G

D

E

G

Y

S

V

W

T

I

I

G

G

E
x
H

N

N

V

A

V

M

I

V

L
x
H

G

G

A

A

R

K

I

V

G

G

R

N

-

Y

-

V

-

I

-

G

-

I

-

S

-

V

-

I

-

L

-

D

-

G

-

A

-

K

-

I

-

G

-

D

H

H

C

V

V

I

I

I

G

G

A

A

N

G

A

A

V

V

-

P

T

N

S

K

D

E

I

I

P

P

D

D

F

Y

C

S

V

L

A

V

V

L

G

G

A

V

P

P

A

G

R

K

V

V

R

R

H

Q

W

L

H

T

P

E

D

E

R

E

K

I

Q

E

W

W

L

T

K

K

active site: H82 (≠ E142), H87 (≠ L147)
binding zinc ion: H87 (≠ L147)

Sites not aligning to the query:

active site: 47, 59, 65, 68, 161
binding zinc ion: 65

1v3wA Structure of ferripyochelin binding protein from pyrococcus horikoshii ot3 (see paper)
31% identity, 30% coverage: 131:193/213 of query aligns to 71:153/173 of 1v3wA

query
sites
1v3wA

P

P

V

T

N

E

I

I

G

G

D

E

G

Y

S

V

W

T

I

I

G

G

E
x
H

N

N

V

A

V

M

I

V

L
x
H

G

G

A

A

R

K

I

V

G

G

R

N

-

Y

-

V

-

I

-

G

-

I

-

S

-

V

-

I

-

L

-

D

-

G

-

A

-

K

-

I

-

G

-

D

H

H

C

V

V

I

I

I

G

G

A

A

N

G

A

A

V

V

-

P

T

N

S

K

D

E

I

I

P

P

D

D

F

Y

C

S

V

L

A

V

V

L

G

G

A

V

P

P

A

G

R

K

V

V

R

R

H

Q

W

L

H

T

P

E

D

E

R

E

K

I

Q

E

W

W

L

T

K

K

active site: H82 (≠ E142), H87 (≠ L147)
binding zinc ion: H87 (≠ L147)

Sites not aligning to the query:

7ra4A Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) in complex with serine (see paper)
52% identity, 25% coverage: 129:181/213 of query aligns to 191:243/243 of 7ra4A

query
sites
7ra4A

R
|
R

R
x
H

P

P

V

-

N

K

I

I

G

G

D

D

G

G

S

V

W

M

I

I

G

G

E

A

N

N

V

A

V

S

I

I

L

L

G

G

A

N

-

I

R

R

I

I

G

G

R

S

H

N

C

A

V

K

I

I

G

G

A

A

N

G

A

S

V

V

T

V

S

S

D

D

I

V

P

P

D

P

F

S

C

I

V

T

A

V

V

V

G

G

A

V

P

P

A

A

R

K

V

P

V

V

binding serine: R191 (= R129), H192 (≠ R130)

Sites not aligning to the query:

binding serine: 91, 92, 156, 157

Query Sequence

>BPHYT_RS31070 FitnessBrowser__BFirm:BPHYT_RS31070
MNIGIFFKVRNRLRTSLQGLIYPFFFARFGARSRILRPIRIDGSDNIAIGTDVFVNNFAW
IETIRAFSVQPRLDIRDRVYIGNSAHIIVTHSIEIGCDVLIADRVYVADYIHGYEDIHTP
VKTQDLIQRRPVNIGDGSWIGENVVILGARIGRHCVIGANAVVTSDIPDFCVAVGAPARV
VRHWHPDRKQWLKGAPSARAACSASVTNEINHA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory