SitesBLAST
Comparing BPHYT_RS31960 FitnessBrowser__BFirm:BPHYT_RS31960 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
41% identity, 95% coverage: 5:305/316 of query aligns to 1:304/304 of 1wwkA
- active site: S96 (≠ N97), R230 (= R231), D254 (= D255), E259 (= E260), H278 (= H279)
- binding nicotinamide-adenine-dinucleotide: V100 (= V101), G146 (= G148), F147 (≠ L149), G148 (= G150), R149 (= R151), I150 (= I152), Y168 (≠ C171), D169 (= D172), P170 (vs. gap), V201 (≠ A202), P202 (= P203), T207 (= T208), T228 (≠ A229), S229 (≠ A230), D254 (= D255), H278 (= H279), G280 (≠ A281)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
40% identity, 89% coverage: 36:316/316 of query aligns to 37:325/334 of 5aovA
- active site: L100 (≠ N97), R241 (= R231), D265 (= D255), E270 (= E260), H288 (= H279)
- binding glyoxylic acid: M52 (≠ R51), L53 (≠ V52), L53 (≠ V52), Y74 (≠ H71), A75 (≠ G72), V76 (= V73), G77 (= G74), R241 (= R231), H288 (= H279)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V73), T104 (≠ V101), F158 (≠ L149), G159 (= G150), R160 (= R151), I161 (= I152), S180 (≠ D172), R181 (≠ N173), A211 (≠ H201), V212 (≠ A202), P213 (= P203), T218 (= T208), I239 (≠ A229), A240 (= A230), R241 (= R231), H288 (= H279), G290 (≠ A281)
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
36% identity, 93% coverage: 21:315/316 of query aligns to 15:319/533 of O43175
- T78 (≠ V73) binding
- R135 (= R131) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (= RI 151:152) binding
- D175 (= D172) binding
- T207 (≠ A202) binding
- CAR 234:236 (≠ AAR 229:231) binding
- D260 (= D255) binding
- V261 (= V256) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (≠ HVAA 279:282) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
37% identity, 90% coverage: 21:305/316 of query aligns to 11:305/305 of 6plfA
7dkmA Phgdh covalently linked to oridonin (see paper)
36% identity, 90% coverage: 21:304/316 of query aligns to 11:304/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (≠ V73), A102 (≠ V101), G148 (= G148), R151 (= R151), I152 (= I152), Y170 (≠ C171), D171 (= D172), P172 (≠ N173), I173 (≠ G174), H202 (= H201), T203 (≠ A202), P204 (= P203), T209 (= T208), C230 (≠ A229), A231 (= A230), R232 (= R231), H279 (= H279), G281 (≠ A281)
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: C14 (≠ S24), K17 (vs. gap), I18 (vs. gap), E293 (≠ T293)
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
36% identity, 90% coverage: 21:304/316 of query aligns to 10:303/303 of 6plgA
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
37% identity, 85% coverage: 21:290/316 of query aligns to 9:288/297 of 6rj3A
6rj2A Crystal structure of phgdh in complex with compound 40 (see paper)
36% identity, 90% coverage: 21:303/316 of query aligns to 7:299/299 of 6rj2A
- binding ~{N}-[(1~{R})-1-[4-(ethanoylsulfamoyl)phenyl]ethyl]-2-methyl-5-phenyl-pyrazole-3-carboxamide: G146 (= G150), I148 (= I152), Y166 (≠ C171), D167 (= D172), P168 (≠ N173), I169 (≠ G174), I170 (vs. gap), H198 (= H201), T199 (≠ A202), L208 (= L211), R228 (= R231)
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
36% identity, 90% coverage: 21:303/316 of query aligns to 10:302/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (≠ V147), G147 (= G148), L148 (= L149), G149 (= G150), R150 (= R151), I151 (= I152), G152 (= G153), D170 (= D172), H201 (= H201), T202 (≠ A202), P203 (= P203)
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
36% identity, 90% coverage: 21:303/316 of query aligns to 10:302/302 of 6rihA
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
37% identity, 85% coverage: 21:290/316 of query aligns to 10:289/301 of 6rj5A
6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
37% identity, 85% coverage: 21:290/316 of query aligns to 9:288/299 of 6cwaA
- binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (= N97), A100 (≠ V101), R149 (= R151), I150 (= I152), Y168 (≠ C171), D169 (= D172), P170 (≠ N173), I171 (≠ G174), H200 (= H201), T201 (≠ A202), P202 (= P203), T207 (= T208), C228 (≠ A229), A229 (= A230), R230 (= R231), H277 (= H279), G279 (≠ A281)
6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
38% identity, 89% coverage: 36:316/316 of query aligns to 36:324/332 of 6biiA
- active site: L99 (≠ N97), R240 (= R231), D264 (= D255), E269 (= E260), H287 (= H279)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (= V73), T103 (≠ V101), G156 (= G148), F157 (≠ L149), G158 (= G150), R159 (= R151), I160 (= I152), A179 (= A175), R180 (= R176), S181 (≠ E177), K183 (≠ L179), V211 (≠ A202), P212 (= P203), E216 (≠ S207), T217 (= T208), V238 (≠ A229), A239 (= A230), R240 (= R231), D264 (= D255), H287 (= H279), G289 (≠ A281)
6plfB Crystal structure of human phgdh complexed with compound 1 (see paper)
37% identity, 85% coverage: 21:290/316 of query aligns to 9:280/292 of 6plfB
- binding 4-{(1S)-1-[(5-chloro-6-{[(5S)-2-oxo-1,3-oxazolidin-5-yl]methoxy}-1H-indole-2-carbonyl)amino]-2-hydroxyethyl}benzoic acid: R141 (= R151), Y160 (≠ C171), D161 (= D172), P162 (≠ N173), I164 (vs. gap), L179 (≠ I188), T193 (≠ A202), P194 (= P203), S198 (= S207), L202 (= L211)
O58320 Glyoxylate reductase; EC 1.1.1.26 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
35% identity, 98% coverage: 6:316/316 of query aligns to 4:325/334 of O58320
2dbqA Crystal structure of glyoxylate reductase (ph0597) from pyrococcus horikoshii ot3, complexed with NADP (i41) (see paper)
35% identity, 98% coverage: 6:316/316 of query aligns to 4:325/333 of 2dbqA
- active site: L100 (≠ N97), R241 (= R231), D265 (= D255), E270 (= E260), H288 (= H279)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V73), T104 (≠ V101), L158 (= L149), G159 (= G150), R160 (≠ I152), I161 (≠ G153), S180 (≠ D172), R181 (≠ N173), T182 (≠ G174), A211 (≠ H201), V212 (≠ A202), P213 (= P203), T218 (= T208), I239 (≠ A229), A240 (= A230), R241 (= R231), D265 (= D255), H288 (= H279), G290 (≠ A281)
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
37% identity, 89% coverage: 36:316/316 of query aligns to 33:321/526 of 3dc2A
Sites not aligning to the query:
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
37% identity, 89% coverage: 36:316/316 of query aligns to 34:322/525 of 3ddnB
3kb6B Crystal structure of d-lactate dehydrogenase from aquifex aeolicus complexed with NAD and lactic acid (see paper)
32% identity, 86% coverage: 35:305/316 of query aligns to 37:320/334 of 3kb6B
- active site: S97 (≠ N97), R231 (= R231), D255 (= D255), E260 (= E260), H294 (= H279)
- binding lactic acid: F49 (≠ I49), S72 (≠ G72), V73 (= V73), G74 (= G74), Y96 (≠ S96), R231 (= R231), H294 (= H279)
- binding nicotinamide-adenine-dinucleotide: V73 (= V73), Y96 (≠ S96), V101 (= V101), G150 (= G150), R151 (= R151), I152 (= I152), D171 (= D172), V172 (≠ N173), P203 (= P203), T229 (≠ A229), A230 (= A230), R231 (= R231), H294 (= H279), A296 (= A281), Y297 (≠ A282)
4e5pA Thermostable phosphite dehydrogenase a176r variant in complex with nad (see paper)
34% identity, 97% coverage: 6:313/316 of query aligns to 4:326/332 of 4e5pA
- active site: L100 (≠ N97), R237 (= R231), D261 (= D255), E266 (= E260), H292 (= H279)
- binding nicotinamide-adenine-dinucleotide: K76 (≠ V73), L100 (≠ N97), T104 (≠ V101), G154 (= G150), A155 (≠ R151), I156 (= I152), A175 (= A175), R176 (= R176), L208 (≠ A202), P209 (= P203), T214 (= T208), P235 (≠ A229), C236 (≠ A230), R237 (= R231), H292 (= H279)
Query Sequence
>BPHYT_RS31960 FitnessBrowser__BFirm:BPHYT_RS31960
MPEKFRVLATSPIHLSALPILEPSCELVVAPDDSEETLRKFVADADALIVRVRLPDDIFD
HAPRLKACVRHGVGLDFIPVERATRADIAVANLPDSNTQAVAEHVVGAILAMARGFDRLP
RAWRNDGWLVRQTFQGIELRDRTVGIVGLGRIGLQVAAALHHGFGMRVLGCDNGAREGLP
SYVDQTSIENVFSGSDFITLHAPLVKSTRHLVNAELLSLAKPGALLVNAARGGLIDDDAL
VAALRSGQIGCAALDVFEPEPLPPDHPYWSIDNVLLTPHVAAFTSEGLVRMSTGAARAVV
EILHGRRPEHLVNEEV
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory